RU2287348C2 - Biocidal preparation and method for biocidal treatment of surface - Google Patents

Abstract

FIELD: medicine, veterinary science, textile and construction industry.

SUBSTANCE: the suggested composition consists of the mixture of polyhexamethylene guanidine (PHMG) of foundation, lime and calcium chloride at certain ratios of ingredients applied. The composition should be obtained due to mixing PHMG-hydrochloride with approximately 10-fold excess of lime. Treatment of surfaces deals with pre-treating the surface under treatment with the purpose to remove microorganisms followed by treating it with 1-5%-PHMG-hydrochloride solution with subsequent application of the mixture of PHMG of foundation, lime and calcium chloride upon it. The innovation enables to obtain a stable and highly efficient biocidal covering.

EFFECT: higher efficiency.

4 cl, 1 ex, 2 tbl

Description

The invention relates to the field of biotechnology, and in particular to preparations having biocidal properties and, therefore, promising for the fight against bacterial and fungal infections of wood, woven and other materials, as well as residential and industrial premises, as well as methods for their use, and may be used in medicine, veterinary medicine, textile and construction industries, as well as related industries.

Currently, to combat infection of the surfaces of objects with microorganisms, drugs such as metaflor, formaldehyde, alkamon, chloride hydrocarbons, etc. are used. (English Pat. 1476730, 1977, class B 27 K 3/50, Pat. Germany 2820409, 1981, class B 27 K 3/50). Processing is carried out by washing surfaces with biocide solutions or by spraying.

However, these drugs do not have a prolonged effect, are dangerous for humans and the environment, ineffective in low concentrations.

One of the most promising groups of biocides is various derivatives of guanidine (Eng. Pat. 821113, 1959, class 15 (2) G, A.S. USSR 1184296, 1983, class D 06 M 14/04, P.A. Gembitsky. Synthesis of Metacide.Chem. Prom. 1984, No. 2, pp. 18-19. Pat. USSR 1687261, 1991, class A 61 L 2/16), combining good biocidal properties with relative low toxicity. Among these derivatives, polyhexamethylene guanidine (PHMG) and its salts with acids are most known, in particular hydrochloride (PHMG-X), gluconate (PHMG-G) and phosphate (PHMG-F) (A.S. USSR 247463, 1968, class A 61 L 2/16, A.S. USSR 1698061, 1991, class B 27 K 3/34), proposed for the fight against bacterial contamination.

It should be noted that the use of biocides based on PHMG requires relatively high concentrations of the drug. So, the antifungicidal effect is noted for PHMG-X at concentrations of 1-7% (A.S. USSR 247463, 1968, class A 61 L 2/16). In addition, there is a high corrosivity of PGMG-X. To carry out biocidal treatment, the PGMG-X preparation is applied to the surface with a brush or using a spray, which is the method closest to the claimed composition for use.

It is known to use in construction as a cement mortar a mixture of PGMG-X (up to 4% by weight) with alkylbenzyldimethylammonium chloride (US Pat. RF No. 2111188, class C 04 B 28/02, 1998). However, the resulting concrete does not differ in high strength characteristics and is environmentally unsafe.

The closest in technical essence to the claimed composition is a cement mortar containing, along with calcium salts (silicates and aluminates), an additive of PGMG-X in an amount of 1-2% by weight of cement (RF Pat. No. 2219141, class 04 04/28/04 , 2002).

The disadvantage of the composition is its low biocidal activity, which also decreases sharply with time, as well as the presence of hydrochloric acid in the composition, which makes the mixture corrosive.

The problem solved by the author was the creation of a less toxic and more effective drug and method of its use.

It was found that this problem is solved by creating a composition consisting of a mixture of polyhexamethylene guanidine base (PHMG-OH), lime and calcium chloride in the following ratio of ingredients (% wt.):

PGMG-OH - 7-15 CaCl ₂ - 3-6 Lime - the rest

The drug is prepared by mixing PHMG-X with an approximately ten-fold excess of lime, which leads to the neutralization of hydrochloric acid and the formation of CaCl ₂ . In this case, PHMG in an alkaline medium passes into PHMG-OH.

Introduction to the composition of PHMG-OH at lower concentrations is ineffective, using a concentration of more than 15% is possible, but impractical for economic reasons. The amount of calcium chloride in the mixture is determined by its yield by reaction. At the same time, it can be expected that the presence of calcium cations with their complexing properties near the N = N bond of PHMG is able to further activate the biocidal properties of the guanidine group.

Significant differences of the claimed composition, which received the name "ANTI-MOLD PLUS", from the prototype is the use of water-insoluble PHMG-OH as an active principle, distributed evenly in the mass of calcium compounds, providing good adhesive properties to most building materials used (concrete, brick, plaster, etc.). P.). At the same time, the absence of free acid, as well as the non-volatility and harmlessness of PHMG-OH, allows the use of a biocidal component in higher concentrations (up to 15% by weight of the composition), which provides protection against any types of microorganisms.

The use of water-insoluble compounds as a biocidal principle required a new technology for coating the biocidal principle. In the course of the experiments, a surface treatment method was developed, which includes preliminary cleaning of the treated surface in order to free it from microorganisms, treating it with a 1-5% solution of PHMG-X, followed by applying the composition “ANTIPLESEN PLUS”

During this treatment, at the first stage, the starting material is freed from fungal spores that could develop in the wall, then the surface is impregnated with a solution of PHMG-X, which when applied to the ANTI-MOLD PLUS surface inside the pores of the wall turns into PHMG-OH, providing a more durable fixing the biocide-containing lime layer on the surface.

Pretreatment depending on the surface condition includes the use of at least one of the following operations:

- cleaning the surface mechanically using card brushes, spatulas, grinding skins, etc .;

- treatment with a sponge with a 0.2-5% solution of HMG-X containing 10-14% hydrogen peroxide (if there are black spots or blotches on the surface);

- heat treatment using a hair dryer, gas burner, blowtorch (to ensure its dehydration and cleaning of microcracks).

Coating "ANTI-MOLD PLUS" on a porous, absorbent surface is carried out using a spray; on a non-absorbent surface (tile, paint, metal, etc.) before ANTI-MOLD PLUS it is applied to the previously applied layer of POLYROL with a soft rag followed by polishing of the polymerized layer.

After the operation is completed, the quality of the protective layer is controlled - its surface, when water is applied to it, must remain dry.

During operation during scheduled cleaning, the surface protective layer can be washed with a damp cloth, avoiding mechanical damage to the layer.

Significant differences of the proposed method from analogues is the preliminary release of the surface from microorganisms, as well as the application of the ANTI-MOLD PLUS layer after treatment with the GMPG-X solution, which ensures the formation of a protective layer with biocidal stability during long-term operation (at least 2-3 years) properties.

The properties of the resulting coating are illustrated by the following example.

Example 1. The study of the disinfectant properties of the product was carried out in accordance with the methods set forth in the collection "Test methods for disinfectants to assess their effectiveness and safety", Moscow, 1998, in accordance with the "Normative indicators of safety and efficacy of funds to be monitored during compulsory certification "No. 01-12 / 75-97 and" Methodological recommendations for determining the virucidal activity of drugs No. 1119-73 of 09/06/73. " The following strains were used as test microorganisms: Escherihia coli 1257, Staphylococcus aureus 906, Pseudomonas aeruginosa 15442, Candida albicans 15, Aspergillus niger.

As the composition used the composition "ANTI-MOLD PLUS", consisting of different amounts of PHMG-OH, calcium chloride and lime.

The surface was preliminarily subjected to a three-stage preliminary treatment and washing with a 2% solution of PHMG-X. The results of biocidal treatment are shown in table 1.

Table 1

% Concentration The average number of microorganisms after treatment M ± m (n = 18) The effectiveness of disinfection,% Pretreatment Method Test object PGMG-OH CaCl ₂ Staphylococcus aureus 906 Metlakh tile 7.0 3.0 one 99,99 2 Brick 10.0 4.0 one 99,99 2 + 3 Painted metal 15.0 6.0 0 one hundred 2 Escherihia coli 1257 Metlakh tile, 7.0 3.0 0 one hundred 2 Concrete 10.0 4.0 3 99,99 2 + 3 Painted metal 15.0 6.0 0 one hundred 2 Pseudomonas aeruginosa 15442 Metlakh tile, 7.0 3.0 0 one hundred 2 Concrete 10.0 4.0 one 99,99 2 + 3 Painted metal 15.0 6.0 0 one hundred 2 Candida albicans 15 Unpainted tree 7.0 3.0 one 99,99 1 + 2 Concrete 10.0 4.0 one 99,99 2 + 3 Painted metal 15.0 6.0 0 one hundred 2 Aspergillus niger Unpainted tree 7.0 3.0 2 99,99 one Concrete 10.0 4.0 0 one hundred 1 + 2 + 3 Plaster 15.0 6.0 one one hundred 2

The processing results after 6 months of storage amounted to (see table 2)

table 2

Test object % Concentration The average number of microorganisms after treatment M ± m (n = 18) The effectiveness of disinfection,% Pretreatment Method PGMG-OH CaCl ₂ Staphylococcus aureus 906 Brick 10.0 4.0 25 ± 10 95 2 + 3 Painted metal 10.0 4.0 10 ± 3 98 Escherihia coli 1257 Concrete 10.0 4.0 20 ± 15 96 2 Painted metal 15.0 6.0 15 ± 4 97 2 + 3 Pseudomonas aeruginosa 15442 Concrete 10.0 4.0 80 ± 15 90 2 Painted metal 15.0 6.0 20 + 2 96 2 + 3 Candida albicans 15 Unpainted tree 10.0 4.0 200 ± 25 80 1 + 2 Concrete 10.0 4.0 50 ± 10 94 2 + 3 Aspergillus niger Unpainted tree 7.0 3.0 150 ± 25 70 one Concrete 10.0 4.0 50 ± 10 82 1 + 2 + 3 Plaster 15.0 6.0 60 ± 10 80 2

Claims (4)

1. A biocidal composition based on derivatives of polyhexamethylene guanidine and calcium derivatives, characterized in that it contains polyhexamethylene guanidine derivatives as polyhexamethylene guanidine derivatives, and a mixture of lime and calcium chloride as calcium derivatives in the following ratio of ingredients, wt.%: Polyhexamethylene guanidine base 7-15 Calcium chloride 3-6 Lime Rest 2. A method of biocidal surface treatment, including surface treatment with an aqueous solution of polyhexamethylene guanidine hydrochloride, characterized in that microorganisms are preliminarily removed from the surface, and after treatment with an aqueous solution of polyhexamethylene guanidine hydrochloride, a mixture of polyhexamethylene guanidine base, lime and calcium chloride is applied to the surface. 3. The method according to claim 2, characterized in that the preliminary surface treatment is carried out using at least one of the following operations: cleaning the surface mechanically using card brushes, spatulas or grinding skins; treating with a sponge a 0.2-5% aqueous solution of polyhexamethylene guanidine hydrochloride containing 10-14% hydrogen peroxide; heat treatment with a hair dryer, gas burner or blowtorch. 4. The method according to claim 2, characterized in that the application of the mixture of polyhexamethylene guanidine base, lime and calcium chloride is carried out on a porous, absorbent surface using a spray, and on a non-absorbing surface on a previously applied layer of POLYROL using a soft rag with subsequent polishing of the obtained layer.