PL2756098T3 - Sposób wytwarzania biblioteki cząsteczek kwasów nukleinowych - Google Patents
Sposób wytwarzania biblioteki cząsteczek kwasów nukleinowychInfo
- Publication number
- PL2756098T3 PL2756098T3 PL12759455T PL12759455T PL2756098T3 PL 2756098 T3 PL2756098 T3 PL 2756098T3 PL 12759455 T PL12759455 T PL 12759455T PL 12759455 T PL12759455 T PL 12759455T PL 2756098 T3 PL2756098 T3 PL 2756098T3
- Authority
- PL
- Poland
- Prior art keywords
- nucleic acid
- acid molecules
- making library
- library
- making
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP11181546A EP2570487A1 (en) | 2011-09-16 | 2011-09-16 | Nucleic acid transcription method |
EP12177647 | 2012-07-24 | ||
PCT/EP2012/068250 WO2013038010A2 (en) | 2011-09-16 | 2012-09-17 | Nucleic acid transcription method |
EP12759455.4A EP2756098B1 (en) | 2011-09-16 | 2012-09-17 | Method for making library of nucleic acid molecules |
Publications (1)
Publication Number | Publication Date |
---|---|
PL2756098T3 true PL2756098T3 (pl) | 2018-12-31 |
Family
ID=46852009
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PL12759455T PL2756098T3 (pl) | 2011-09-16 | 2012-09-17 | Sposób wytwarzania biblioteki cząsteczek kwasów nukleinowych |
Country Status (11)
Country | Link |
---|---|
US (3) | US10612018B2 (pl) |
EP (2) | EP2756098B1 (pl) |
KR (1) | KR102087062B1 (pl) |
CN (1) | CN103930570B (pl) |
AU (1) | AU2012307282B2 (pl) |
CA (1) | CA2848240C (pl) |
DK (1) | DK2756098T3 (pl) |
ES (1) | ES2686043T3 (pl) |
LT (1) | LT2756098T (pl) |
PL (1) | PL2756098T3 (pl) |
WO (1) | WO2013038010A2 (pl) |
Families Citing this family (48)
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CA2848240C (en) | 2011-09-16 | 2020-09-29 | Lexogen Gmbh | Nucleic acid transcription method |
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EP2877593B1 (en) * | 2012-07-26 | 2018-07-18 | Illumina, Inc. | Compositions and methods for the amplification of nucleic acids |
AU2013359293B2 (en) * | 2012-12-13 | 2017-11-02 | Synthetic Genomics, Inc. | PEG-mediated assembly of nucleic acid molecules |
EP3480321B8 (en) | 2013-08-28 | 2021-03-10 | Becton, Dickinson and Company | Massively parallel single cell analysis |
EP2921556A1 (en) * | 2014-03-21 | 2015-09-23 | Lexogen GmbH | Copy number preserving RNA analysis method |
WO2016058121A1 (zh) * | 2014-10-13 | 2016-04-21 | 深圳华大基因科技有限公司 | 一种核酸片段化方法和序列组合 |
WO2016058173A1 (zh) * | 2014-10-17 | 2016-04-21 | 深圳华大基因研究院 | 一种用于核酸随机片段化的引物及核酸随机片段化方法 |
CN104560974B (zh) * | 2014-12-26 | 2017-08-25 | 光明乳业股份有限公司 | 获得嗜热链球菌特异性序列的方法及其使用的半随机引物 |
US9727810B2 (en) | 2015-02-27 | 2017-08-08 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
ES2934982T3 (es) | 2015-03-30 | 2023-02-28 | Becton Dickinson Co | Métodos para la codificación con códigos de barras combinatorios |
US11390914B2 (en) | 2015-04-23 | 2022-07-19 | Becton, Dickinson And Company | Methods and compositions for whole transcriptome amplification |
US10619186B2 (en) | 2015-09-11 | 2020-04-14 | Cellular Research, Inc. | Methods and compositions for library normalization |
US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
CN109328235A (zh) * | 2016-06-23 | 2019-02-12 | 国立研究开发法人理化学研究所 | 一步逆转录模板转换pcr |
IL265478B2 (en) | 2016-09-26 | 2024-10-01 | Becton Dickinson Co | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
CN108330186A (zh) * | 2017-01-18 | 2018-07-27 | 深圳华大智造科技有限公司 | 一种核酸测序方法、反应体系和试剂盒 |
EP3577232A1 (en) | 2017-02-01 | 2019-12-11 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
CA3052209A1 (en) * | 2017-02-27 | 2018-08-30 | Bioo Scientific Corporation | Methods and kits for depleting undesired nucleic acids |
US11193153B2 (en) | 2017-03-20 | 2021-12-07 | President And Fellows Of Harvard College | Programmable nucleic acid synthesis cascade |
US10676779B2 (en) | 2017-06-05 | 2020-06-09 | Becton, Dickinson And Company | Sample indexing for single cells |
EP3724331A4 (en) * | 2017-12-15 | 2021-09-01 | Baylor College of Medicine | METHODS AND COMPOSITIONS FOR AMPLIFICATION OF RNAM |
US11773441B2 (en) | 2018-05-03 | 2023-10-03 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
JP7358388B2 (ja) | 2018-05-03 | 2023-10-10 | ベクトン・ディキンソン・アンド・カンパニー | 反対側の転写物末端における分子バーコーディング |
JP2022511398A (ja) | 2018-10-01 | 2022-01-31 | ベクトン・ディキンソン・アンド・カンパニー | 5’転写物配列の決定 |
US11932849B2 (en) | 2018-11-08 | 2024-03-19 | Becton, Dickinson And Company | Whole transcriptome analysis of single cells using random priming |
EP3894552A1 (en) | 2018-12-13 | 2021-10-20 | Becton, Dickinson and Company | Selective extension in single cell whole transcriptome analysis |
EP3666904A1 (en) | 2018-12-14 | 2020-06-17 | Lexogen GmbH | Nucleic acid amplification and identification method |
CN113574178B (zh) | 2019-01-23 | 2024-10-29 | 贝克顿迪金森公司 | 与抗体关联的寡核苷酸 |
WO2020167920A1 (en) * | 2019-02-14 | 2020-08-20 | Cellular Research, Inc. | Hybrid targeted and whole transcriptome amplification |
US11939622B2 (en) | 2019-07-22 | 2024-03-26 | Becton, Dickinson And Company | Single cell chromatin immunoprecipitation sequencing assay |
EP4407041A3 (en) | 2019-11-08 | 2024-09-25 | Becton Dickinson and Company | Using random priming to obtain full-length v(d)j information for immune repertoire sequencing |
CN110846724B (zh) * | 2019-12-03 | 2023-04-28 | 江西海普洛斯医学检验实验室有限公司 | 构建mRNA链特异文库的方法及试剂盒 |
EP4085135A4 (en) | 2019-12-31 | 2023-08-02 | Singular Genomics Systems, Inc. | Polynucleotide barcodes for long read sequencing |
EP4090763B1 (en) | 2020-01-13 | 2024-12-04 | Becton Dickinson and Company | Methods and compositions for quantitation of proteins and rna |
CN115335520A (zh) | 2020-01-29 | 2022-11-11 | 贝克顿迪金森公司 | 用于通过测序对单细胞进行空间映射的条形码化的孔 |
EP4111168A1 (en) | 2020-02-25 | 2023-01-04 | Becton Dickinson and Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
EP4150118A1 (en) | 2020-05-14 | 2023-03-22 | Becton Dickinson and Company | Primers for immune repertoire profiling |
WO2021247593A1 (en) | 2020-06-02 | 2021-12-09 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
CN111944886B (zh) * | 2020-08-24 | 2022-09-27 | 北京梓熙生物科技有限公司 | 一种合成寡聚脱氧核糖核苷酸的方法和试剂盒 |
EP4247967A1 (en) | 2020-11-20 | 2023-09-27 | Becton, Dickinson and Company | Profiling of highly expressed and lowly expressed proteins |
CN114606575B (zh) * | 2020-12-03 | 2025-02-14 | 广东菲鹏生物有限公司 | 组合物及文库构建方法 |
US20230002805A1 (en) * | 2021-06-25 | 2023-01-05 | Enzo Biochem, Inc. | Use of organic cationic compounds to accelerate nucleic acid hybridization, synthesis, and amplification |
WO2022272150A2 (en) * | 2021-06-25 | 2022-12-29 | Singular Genomics Systems, Inc. | Linked transcript sequencing |
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CA2848240C (en) | 2011-09-16 | 2020-09-29 | Lexogen Gmbh | Nucleic acid transcription method |
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-
2012
- 2012-09-17 CA CA2848240A patent/CA2848240C/en active Active
- 2012-09-17 DK DK12759455.4T patent/DK2756098T3/en active
- 2012-09-17 PL PL12759455T patent/PL2756098T3/pl unknown
- 2012-09-17 KR KR1020147010187A patent/KR102087062B1/ko active IP Right Grant
- 2012-09-17 CN CN201280056141.4A patent/CN103930570B/zh active Active
- 2012-09-17 EP EP12759455.4A patent/EP2756098B1/en active Active
- 2012-09-17 AU AU2012307282A patent/AU2012307282B2/en active Active
- 2012-09-17 LT LTEP12759455.4T patent/LT2756098T/lt unknown
- 2012-09-17 US US14/241,311 patent/US10612018B2/en active Active
- 2012-09-17 WO PCT/EP2012/068250 patent/WO2013038010A2/en active Application Filing
- 2012-09-17 ES ES12759455.4T patent/ES2686043T3/es active Active
- 2012-09-17 EP EP17184341.0A patent/EP3263718A1/en not_active Ceased
-
2017
- 2017-08-10 US US15/674,472 patent/US11021705B2/en active Active
-
2021
- 2021-04-09 US US17/226,688 patent/US20210332355A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
AU2012307282B2 (en) | 2018-03-15 |
EP2756098A2 (en) | 2014-07-23 |
WO2013038010A2 (en) | 2013-03-21 |
EP2756098B1 (en) | 2018-06-06 |
EP3263718A1 (en) | 2018-01-03 |
CN103930570B (zh) | 2018-01-09 |
KR102087062B1 (ko) | 2020-03-11 |
AU2012307282A1 (en) | 2014-03-20 |
CA2848240A1 (en) | 2013-03-21 |
US10612018B2 (en) | 2020-04-07 |
ES2686043T3 (es) | 2018-10-16 |
US20170342409A1 (en) | 2017-11-30 |
KR20140065448A (ko) | 2014-05-29 |
DK2756098T3 (en) | 2018-09-03 |
CN103930570A (zh) | 2014-07-16 |
US20210332355A1 (en) | 2021-10-28 |
LT2756098T (lt) | 2018-09-10 |
US20150152409A1 (en) | 2015-06-04 |
WO2013038010A3 (en) | 2013-05-30 |
US11021705B2 (en) | 2021-06-01 |
CA2848240C (en) | 2020-09-29 |
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