[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

PL2504421T3 - Sposoby i kompozycje do wytwarzania skwalenu z użyciem drożdży - Google Patents

Sposoby i kompozycje do wytwarzania skwalenu z użyciem drożdży

Info

Publication number
PL2504421T3
PL2504421T3 PL10782791T PL10782791T PL2504421T3 PL 2504421 T3 PL2504421 T3 PL 2504421T3 PL 10782791 T PL10782791 T PL 10782791T PL 10782791 T PL10782791 T PL 10782791T PL 2504421 T3 PL2504421 T3 PL 2504421T3
Authority
PL
Poland
Prior art keywords
yeast
compositions
methods
producing squalene
squalene
Prior art date
Application number
PL10782791T
Other languages
English (en)
Inventor
Keith A. Walker
Mark E. Knuth
Noel M. Fong
Peter R. Beetham
Original Assignee
Nucelis Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nucelis Llc filed Critical Nucelis Llc
Publication of PL2504421T3 publication Critical patent/PL2504421T3/pl

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • C12N1/165Yeast isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/10Plasmid DNA
    • C12N2800/102Plasmid DNA for yeast
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • C12R2001/72Candida
    • C12R2001/73Candida lipolytica

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
PL10782791T 2009-11-23 2010-11-22 Sposoby i kompozycje do wytwarzania skwalenu z użyciem drożdży PL2504421T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26377509P 2009-11-23 2009-11-23
PCT/US2010/057668 WO2011063350A2 (en) 2009-11-23 2010-11-22 Methods and compositions for producing squalene using yeast
EP10782791.7A EP2504421B1 (en) 2009-11-23 2010-11-22 Methods and compositions for producing squalene using yeast

Publications (1)

Publication Number Publication Date
PL2504421T3 true PL2504421T3 (pl) 2017-12-29

Family

ID=43587556

Family Applications (1)

Application Number Title Priority Date Filing Date
PL10782791T PL2504421T3 (pl) 2009-11-23 2010-11-22 Sposoby i kompozycje do wytwarzania skwalenu z użyciem drożdży

Country Status (16)

Country Link
US (3) US8470568B2 (pl)
EP (2) EP2504421B1 (pl)
JP (2) JP5978132B2 (pl)
KR (1) KR101951051B1 (pl)
CN (2) CN107058145A (pl)
AU (2) AU2010321722B2 (pl)
CA (1) CA2781303A1 (pl)
DK (2) DK2504421T3 (pl)
EA (1) EA201290199A1 (pl)
ES (2) ES2625154T3 (pl)
HU (1) HUE034245T2 (pl)
IL (1) IL219962A0 (pl)
PL (1) PL2504421T3 (pl)
PT (1) PT2504421T (pl)
UA (1) UA111813C2 (pl)
WO (1) WO2011063350A2 (pl)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2504421T3 (en) * 2009-11-23 2017-05-22 Nucelis Inc METHODS AND COMPOSITIONS FOR PREPARING SQUAL USING Yeast
WO2011146833A1 (en) * 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
ES2699747T3 (es) * 2011-04-29 2019-02-12 Danisco Us Inc Microorganismos recombinantes para la producción potenciada de mevalonato, isopreno e isoprenoides
US9957515B2 (en) 2013-03-15 2018-05-01 Cibus Us Llc Methods and compositions for targeted gene modification
ES2921207T3 (es) * 2013-03-15 2022-08-19 Cibus Us Llc Procedimientos y composiciones para aumentar la eficiencia de la modificación genética direccionada utilizando la reparación genética mediada por oligonucleótidos
GB2518927A (en) * 2013-05-21 2015-04-08 Univ Swansea The production of biomolecules using yeast
US10280406B2 (en) 2014-04-09 2019-05-07 Adeka Corporation Mutant enzyme and production method for terpenoid using said mutant enzyme
JP6475336B2 (ja) * 2014-08-21 2019-02-27 チュン−アン ユニヴァーシティ インダストリー−アカデミー コーポレーション ファウンデーションChung−Ang University Industry−Academy Cooperation Foundation rDNA NTS基盤の遺伝子多重挿入カセットセット、及びそれを用いたGRAS等級の組換え酵母菌株
CN104560731B (zh) * 2014-12-26 2018-10-09 中国科学院青岛生物能源与过程研究所 一种高产角鲨烯的类酵母菌及其应用
JP6644797B2 (ja) * 2015-09-18 2020-02-12 神戸天然物化学株式会社 形質転換体、およびテルペノイドの製造方法
AU2016344131A1 (en) 2015-10-29 2018-05-10 Senomyx, Inc. High intensity sweeteners
SG11201910136RA (en) 2017-05-03 2019-11-28 Firmenich Incorporated Methods for making high intensity sweeteners
CN110452931A (zh) * 2018-05-08 2019-11-15 中国科学院微生物研究所 一种提高酵母中角鲨烯含量的方法
CN109666683B (zh) * 2019-02-27 2021-10-29 昆明理工大学 乙酰辅酶A乙酰转移酶基因RKAcaT2及其应用
CN110607247B (zh) * 2019-08-15 2021-06-08 宜昌东阳光生化制药有限公司 一种提高酿酒酵母合成角鲨烯能力的方法
CN110628795B (zh) * 2019-09-30 2021-07-16 北京市农林科学院 以失活的筛选剂抗性基因为报告体系的a·g碱基替换的细胞富集技术及其应用
CN113234610B (zh) * 2021-03-05 2023-06-13 江南大学 一种合成角鲨烯的酿酒酵母菌株及其应用
CN113444701B (zh) * 2021-06-30 2024-01-30 江南大学 酿酒酵母内源角鲨烯单加氧酶突变体及应用
CN114058525B (zh) * 2021-10-29 2024-09-20 湖北冠众通科技有限公司 一种产角鲨烯基因工程菌及其构建方法与应用
CN117487681A (zh) * 2023-11-16 2024-02-02 湖南农业大学 一种生产角鲨烯的酵母工程菌及其应用

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US625586A (en) 1899-05-23 Cooking utensil
US411969A (en) 1889-10-01 Apparatus for forming glasses
JPS5834114B2 (ja) * 1975-04-17 1983-07-25 フジセイユ カブシキガイシヤ カカオバタ−ダイヨウシノセイゾウホウ
US4628033A (en) * 1983-10-06 1986-12-09 Pfizer Inc. Novel host strain for transformation of Yarrowia lipolytica
US5100792A (en) * 1984-11-13 1992-03-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues
US4945050A (en) * 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5302523A (en) * 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5204253A (en) * 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
US5460949A (en) * 1990-11-15 1995-10-24 Amoco Corporation Method and composition for increasing the accumulation of squalene and specific sterols in yeast
DE4216134A1 (de) 1991-06-20 1992-12-24 Europ Lab Molekularbiolog Synthetische katalytische oligonukleotidstrukturen
IT230274Y1 (it) 1993-06-11 1999-06-02 Silc Spa Pannolone assorbente sagomato per incontinenza
EP0733059B1 (en) 1993-12-09 2000-09-13 Thomas Jefferson University Compounds and methods for site-directed mutations in eukaryotic cells
ES2203635T3 (es) 1994-04-27 2004-04-16 Novartis Ag Nucleosidos y oligonucleotidos con grupos 2'-eter.
US5780296A (en) 1995-01-17 1998-07-14 Thomas Jefferson University Compositions and methods to promote homologous recombination in eukaryotic cells and organisms
EP0747484A1 (en) 1995-06-08 1996-12-11 Institut National De La Recherche Agronomique (Inra) Upstream activator sequences and recombinant promoter sequences functional in yarrowia and vectors containing them
US5888983A (en) * 1996-05-01 1999-03-30 Thomas Jefferson University Method and oligonucleobase compounds for curing diseases caused by mutations
US5760012A (en) 1996-05-01 1998-06-02 Thomas Jefferson University Methods and compounds for curing diseases caused by mutations
US5731181A (en) 1996-06-17 1998-03-24 Thomas Jefferson University Chimeric mutational vectors having non-natural nucleotides
KR20010020375A (ko) 1997-04-30 2001-03-15 무레 미카엘 에프. 간세포에서 유전적 병소를 교정하기 위한 재조합에 의해 만들어진 올리고뉴클레오염기의 in vivo에서의 용도
US6524613B1 (en) 1997-04-30 2003-02-25 Regents Of The University Of Minnesota Hepatocellular chimeraplasty
NZ502929A (en) 1997-08-05 2001-09-28 Kimeragen Inc The use of mixed duplex oligonucleotides to effect localized genetic changes in plants
US6010907A (en) 1998-05-12 2000-01-04 Kimeragen, Inc. Eukaryotic use of non-chimeric mutational vectors
US6004804A (en) 1998-05-12 1999-12-21 Kimeragen, Inc. Non-chimeric mutational vectors
US6531303B1 (en) * 1998-07-06 2003-03-11 Arkion Life Sciences Llc Method of producing geranylgeraniol
US6271360B1 (en) * 1999-08-27 2001-08-07 Valigen (Us), Inc. Single-stranded oligodeoxynucleotide mutational vectors
US6822142B2 (en) * 2001-01-05 2004-11-23 Monsanto Company Transgenic plants containing altered levels of steroid compounds
CN101023181A (zh) * 2004-05-21 2007-08-22 加利福尼亚大学董事会 提高类异戊二烯化合物的产生的方法
EP2371967B1 (en) * 2005-03-18 2015-06-03 DSM IP Assets B.V. Production of carotenoids in oleaginous yeast and fungi
US20080193937A1 (en) * 2005-03-30 2008-08-14 Jitao Zou Identification of a Sterol Acyltransferase Gene
US8367395B2 (en) * 2006-09-28 2013-02-05 Dsm Ip Assets B.V. Production of sterols in oleaginous yeast and fungi
US9119132B2 (en) * 2007-10-10 2015-08-25 Qualcomm Incorporated Efficient system identification schemes for communication systems
US8425310B2 (en) 2008-04-18 2013-04-23 Konami Gaming, Inc. System and method for tracking patrons non-gaming casino spend
KR20180021928A (ko) * 2008-05-23 2018-03-05 누셀리스 엘엘씨 효모를 사용한 스쿠알렌 생산
CA2735404C (en) 2008-08-28 2013-04-16 Novartis Ag Production of squalene from hyper-producing yeasts
DK2504421T3 (en) * 2009-11-23 2017-05-22 Nucelis Inc METHODS AND COMPOSITIONS FOR PREPARING SQUAL USING Yeast

Also Published As

Publication number Publication date
HUE034245T2 (en) 2018-02-28
JP2013511282A (ja) 2013-04-04
EP3219815B1 (en) 2019-10-02
US9540662B2 (en) 2017-01-10
CA2781303A1 (en) 2011-05-26
JP6479711B2 (ja) 2019-03-06
CN102666837A (zh) 2012-09-12
AU2016204319A1 (en) 2016-07-14
AU2010321722B2 (en) 2016-03-24
ES2625154T3 (es) 2017-07-18
KR101951051B1 (ko) 2019-02-21
US11952577B2 (en) 2024-04-09
US20110124072A1 (en) 2011-05-26
EP2504421A2 (en) 2012-10-03
EP3219815A2 (en) 2017-09-20
WO2011063350A2 (en) 2011-05-26
US20140134696A1 (en) 2014-05-15
AU2010321722A1 (en) 2012-07-12
WO2011063350A3 (en) 2011-12-29
DK2504421T3 (en) 2017-05-22
JP2017006121A (ja) 2017-01-12
EP2504421B1 (en) 2017-02-15
DK3219815T3 (da) 2019-12-16
KR20120086328A (ko) 2012-08-02
US20170145429A1 (en) 2017-05-25
ES2760911T3 (es) 2020-05-18
PT2504421T (pt) 2017-05-25
EA201290199A1 (ru) 2014-05-30
US8470568B2 (en) 2013-06-25
JP5978132B2 (ja) 2016-08-24
CN107058145A (zh) 2017-08-18
UA111813C2 (uk) 2016-06-24
EP3219815A3 (en) 2017-11-22
IL219962A0 (en) 2012-07-31

Similar Documents

Publication Publication Date Title
IL219962A0 (en) Methods and compositions for producing squalene using yeast
PL2290051T3 (pl) Mikroorganizm produkujący O-acetylohomoserynę i sposób wytwarzania O-acetylohomoseryny z użyciem mikroorganizmu
IL247238A0 (en) Methods for preparing vesicles and preparations including them
ZA201006944B (en) Compositions and methods for preparing and using same
HK1164832A1 (en) Piceatannol-containing composition and method of producing piceatannol- containing composition
IL216305A0 (en) Solid forms and process for preparing
PL2292783T3 (pl) Mikroorganizm produkujący O-acetylo-homoserynę i sposób wytwarzania O-acetylohomoseryny z użyciem mikroorganizmu
GB0903299D0 (en) Composition and methods
IL216173A (en) Method of fermentation
EP2399595A4 (en) IMMUNOSTIMULATING COMPOSITION AND METHOD FOR THE PRODUCTION THEREOF
IL233881A0 (en) Production of squalene using yeast
EP2515940A4 (en) IMMUNOGENIC COMPOSITIONS AND CORRESPONDING METHODS
GB0912468D0 (en) Composition and method
ZA201106240B (en) Method and means for producing tissues and tissues obtained
HRP20130525T1 (en) Method and device for producing construction elements
PL392720A1 (pl) Kompozycja piwa oraz sposób wytwarzania piwa
ZA201204133B (en) Health-beneficial preparation and production method
ZA201304127B (en) Oral compositions and method for producing thereof
IL218325A0 (en) Methods and compositions for producing plants
GB0901494D0 (en) Compositions and Methods
ZA201304126B (en) Oral compositions and method for producing thereof
ZA201304088B (en) Oral compositions and method for producing thereof
GB0901667D0 (en) Composition and method
PT2443227T (pt) Levedura, método de preparação, composição, equipamento e utilizações
HUP0900794A2 (en) Process for producing prasurgel and its intermediate