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PE20150336A1 - METHODS AND COMPOSITIONS FOR RNA-DIRECTED MODIFICATION OF TARGET DNA AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION - Google Patents

METHODS AND COMPOSITIONS FOR RNA-DIRECTED MODIFICATION OF TARGET DNA AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION

Info

Publication number
PE20150336A1
PE20150336A1 PE2014002211A PE2014002211A PE20150336A1 PE 20150336 A1 PE20150336 A1 PE 20150336A1 PE 2014002211 A PE2014002211 A PE 2014002211A PE 2014002211 A PE2014002211 A PE 2014002211A PE 20150336 A1 PE20150336 A1 PE 20150336A1
Authority
PE
Peru
Prior art keywords
rna
target
target dna
methods
dna
Prior art date
Application number
PE2014002211A
Other languages
Spanish (es)
Inventor
Emmanuelle Charpentier
Jennifer A Doudna
Martin Jinek
Krzysztof Chylinski
Cate James Harrison Doudna
Wendell Lim
Lei Qi
Original Assignee
Univ California
Univ Vienna
Emmanuelle Charpentier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=49624232&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PE20150336(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ California, Univ Vienna, Emmanuelle Charpentier filed Critical Univ California
Publication of PE20150336A1 publication Critical patent/PE20150336A1/en

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Abstract

La presente descripcion proporciona un ARN dirigido a ADN que comprende una secuencia objetivo y, junto con un polipeptido modificador, proporciona una modificacion especifica del sitio de un ADN objetivo y/o un polipeptido asociado con el ADN objetivo. La presente descripcion proporciona ademas polipeptidos modificadores especificos del sitio. La presente descripcion proporciona ademas metodos para la modificacion especifica del sitio de un ADN objetivo y/o un polipeptido asociado con el ADN objetivo. La presente descripcion proporciona metodos para modular la transcripcion de un acido nucleico objetivo en una celula objetivo, que generalmente involucra poner en contacto el acido nucleico objetivo con un polipeptido Cas9 enzimaticamente inactivo y un ARN dirigido a ADN. Se proporcionan tambien kits y composiciones para llevar a cabo los metodos. La presente descripcion proporciona celulas modificadas geneticamente que producen Cas9; y organismos multicelulares no humanos Cas9 transgenicosThe present disclosure provides a DNA targeting RNA comprising a target sequence and, together with a modifying polypeptide, provides a site specific modification of a target DNA and / or a polypeptide associated with the target DNA. The present description further provides site-specific modifying polypeptides. The present description further provides methods for site-specific modification of a target DNA and / or a polypeptide associated with the target DNA. The present disclosure provides methods for modulating the transcription of a target nucleic acid in a target cell, which generally involves contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA targeting RNA. Kits and compositions are also provided for carrying out the methods. The present description provides genetically modified cells that produce Cas9; and non-human multicellular Cas9 transgenic organisms

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