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LTIP237A - Po438, a new calcium-regulated promotor - Google Patents

Po438, a new calcium-regulated promotor

Info

Publication number
LTIP237A
LTIP237A LTIP237A LTIP237A LTIP237A LT IP237 A LTIP237 A LT IP237A LT IP237 A LTIP237 A LT IP237A LT IP237 A LTIP237 A LT IP237A LT IP237 A LTIP237 A LT IP237A
Authority
LT
Lithuania
Prior art keywords
polypeptide
enzyme
new calcium
recombinant vector
operatively linked
Prior art date
Application number
LTIP237A
Inventor
Jose Daza Ortega
Jose Antonio Gil
Tomas Vigal Garcia
Juan Francisco Martin
Original Assignee
Serono Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Serono Lab filed Critical Serono Lab
Publication of LTIP237A publication Critical patent/LTIP237A/en
Publication of LT3133B publication Critical patent/LT3133B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • C12Y113/11001—Catechol 1,2-dioxygenase (1.13.11.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • C12Y113/11002—Catechol 2,3-dioxygenase (1.13.11.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Dental Preparations (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Hydrogenated Pyridines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

There is disclosed a new calcium-regulated promoter to be used for increasing production of extracellular enzymes, or heterologous polypeptides, a recombinant vector that includes the DNA sequence of the promoter operatively linked to a DNA encoding said enzyme or polypeptide and a host organism transformed with the recombinant vector that includes the promoter operatively linked to the DNA encoding said enzyme or polypeptide. The present invention further relates to Streptomyces expression systems and methods for expressing foreign DNA sequences in Streptomyces and for secreting to the surrounding medium polypeptides and proteins coded for by those foreign DNA sequences.
LTIP237A 1991-12-12 1992-12-11 Po438, a new calcium-regulated promotor LT3133B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9102765 1991-12-12

Publications (2)

Publication Number Publication Date
LTIP237A true LTIP237A (en) 1994-07-15
LT3133B LT3133B (en) 1995-01-31

Family

ID=8274462

Family Applications (1)

Application Number Title Priority Date Filing Date
LTIP237A LT3133B (en) 1991-12-12 1992-12-11 Po438, a new calcium-regulated promotor

Country Status (15)

Country Link
US (2) US5385841A (en)
EP (1) EP0546833B1 (en)
JP (1) JP3340164B2 (en)
AT (1) ATE154392T1 (en)
AU (1) AU661073B2 (en)
CA (1) CA2084351C (en)
DE (1) DE69220336T2 (en)
DK (1) DK0546833T3 (en)
ES (1) ES2103902T3 (en)
GR (1) GR3024625T3 (en)
IL (1) IL103975A (en)
LT (1) LT3133B (en)
LV (1) LV10728B (en)
RU (1) RU2112802C1 (en)
ZA (1) ZA929284B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA929284B (en) * 1991-12-12 1993-06-02 Serono Lab P0438, an new calcium-regulated promoter.
US6381353B1 (en) 1996-08-30 2002-04-30 Marvin Weiss System for counting colonies of micro-organisms in petri dishes and other culture media

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8315579D0 (en) * 1983-06-07 1983-07-13 Biogen Nv Streptomyces expression systems
US4766066A (en) * 1984-09-27 1988-08-23 Eli Lilly And Company Method of using bacteriophage lambda p1 promoter to produce a functional polypeptide in streptomyces
US4745056A (en) * 1984-10-23 1988-05-17 Biotechnica International, Inc. Streptomyces secretion vector
DE3536182A1 (en) * 1985-10-10 1987-04-16 Hoechst Ag PROMOTOR ARRANGEMENT FOR STREPTOMYCETE VECTORS
US5200327A (en) * 1985-11-06 1993-04-06 Cangene Corporation Expression system for the secretion of bioactive human granulocyte macrophage colony stimulating factor (GM-CSF) and other heterologous proteins from streptomyces
WO1988007079A1 (en) * 1987-03-09 1988-09-22 Cetus Corporation Expression of heterologous genes in streptomyces species
US5118617A (en) * 1989-05-19 1992-06-02 Applied Research Systems Ars Holding N.V. Saf polypeptide, gene coding therefor, saf promoter and expression methods for expressing foreign dna sequences in streptomyces
ZA929284B (en) * 1991-12-12 1993-06-02 Serono Lab P0438, an new calcium-regulated promoter.

Also Published As

Publication number Publication date
LT3133B (en) 1995-01-31
GR3024625T3 (en) 1997-12-31
DE69220336D1 (en) 1997-07-17
EP0546833A2 (en) 1993-06-16
AU661073B2 (en) 1995-07-13
JP3340164B2 (en) 2002-11-05
RU2112802C1 (en) 1998-06-10
LV10728A (en) 1995-06-20
CA2084351A1 (en) 1993-06-13
ZA929284B (en) 1993-06-02
ATE154392T1 (en) 1997-06-15
US5660999A (en) 1997-08-26
CA2084351C (en) 2007-04-10
ES2103902T3 (en) 1997-10-01
IL103975A0 (en) 1993-05-13
DE69220336T2 (en) 1997-09-25
AU2979192A (en) 1993-06-17
DK0546833T3 (en) 1997-09-15
JPH05336972A (en) 1993-12-21
US5385841A (en) 1995-01-31
LV10728B (en) 1995-12-20
EP0546833A3 (en) 1994-04-06
IL103975A (en) 1998-08-16
EP0546833B1 (en) 1997-06-11

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Legal Events

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MM9A Lapsed patents

Effective date: 20081211