KR970062693A - Aggregation reaction and separation vessel - Google Patents

Abstract

A vessel to perform hemagglutination assay. The barrier retains the reactants in the upper chamber during incubation and, when applied, forces reagents into the lower chamber containing the matrix for flocculation separation.

Description

Aggregation reaction and separation vessel

Since this is an open matter, no full text was included.

1 shows a reaction and separation vessel using an insert having a small aperture placed in the upper reaction chamber.

FIG. 2 shows four vessels without any inlet, one vessel containing the inlet shown in FIG. 1 (second from left), and one vessel (third from left) showing the vessel containing the reactants. Cassette top view of two reaction vessels.

3 is a cross-sectional view of the cassette of the reaction vessel taken along line 3-3 of FIG.

4 is a side view of the cassette of the reaction vessel.

Claims (26)

a) an upper chamber having a hole for receiving a fluid reactant; b) a lower chamber containing a matrix that receives fluid from the upper chamber and separates the aggregates; c) permit passage of fluid from the upper chamber to the lower chamber under pressure above atmospheric pressure, have means for retaining the fluid in the upper chamber under normal gravity and atmospheric conditions, and separate the upper chamber from the lower chamber and have a spiral structure Coagulation assay vessel comprising a barrier. The coagulation analysis container according to claim 1, wherein the helix structure has a column wall and a passage described by a screw thread, which is small enough to retain fluid in the upper chamber under atmospheric pressure at normal gravity. 3. The aggregation assay vessel of claim 2 wherein the helix structure comprises a helix insert. a) an upper reaction chamber having apertures formed in the spiral insert structure and holes for receiving fluid reagents such that the fluid can be retained in the upper reaction chamber under gravity and atmospheric pressure; And b) a lower chamber in communication with the upper chamber through the helical insert and comprising a separation matrix separating the aggregates. 5. Agglutination assay container according to claim 4, wherein the helix insert is polypropylene or flexorite. a) a second chamber for holding and receiving fluid samples and reagents; b) a coagulation analysis vessel comprising a second chamber in communication with the first chamber to receive fluid from the first chamber and having a separation matrix separating the aggregates; c) a coagulation analysis vessel comprising a barrier having a helix insert to separate said first and second chambers under pressure greater than atmospheric pressure, said first and second chambers being capable of preventing fluid from flowing from the first chamber to the second chamber. 7. The coagulation assay container of claim 6, wherein the helix insert includes a small diameter passageway allowing fluid to remain in the first chamber under normal gravity and atmospheric pressure. 8. The aggregation assay vessel of claim 7 wherein the diameter of the helix insert is in the range of about 0.100 to 0.140 inches. The agglutination assay container of claim 8 wherein the diameter of the helix insert axis is between about 0.03 and 0.90 inches. 10. The aggregation assay vessel of claim 9 wherein the helix insert has about 6 to 30 threads per inch. a) an upper chamber having a hole for receiving a fluid reactant; b) a lower chamber comprising a matrix for separating the aggregates and arranged to receive fluid from the upper chamber; c) a coagulation analysis vessel which allows passage of fluid from the upper chamber to the lower chamber under pressure above atmospheric pressure and has a means for retaining the fluid in the upper chamber under normal gravity and atmospheric pressure and including a barrier separating the upper chamber from the lower chamber . 12. The coagulation assay container of claim 11 having a small central hole to retain fluid in the upper chamber under normal gravity and atmospheric pressure. 13. The coagulation assay container of claim 12, wherein the diaphragm is silicone rubber. a) an upper chamber having a hole for receiving a fluid reactant; b) a lower chamber arranged to receive a fluid from the upper chamber and to receive a matrix for separating the aggregates; And c) a barrier separating the upper chamber from the lower chamber with a means for retaining the fluid in the upper chamber under normal gravity and atmospheric pressure under a pressure greater than atmospheric pressure. Coagulation assay vessel. 15. The coagulation assay container of claim 14, wherein the porous stopper has a through passage small enough to retain fluid in the upper chamber under normal gravity and atmospheric pressure. 16. The coagulite container of claim 15, wherein the porous plug is polypropylene. A liner that prevents cross-contamination of reagents or solutions from one column of agglutination assay cassette to another column of the cassette, wherein the liner has at least one hole integrally with the body and the body, and the conical member is one at a narrowed peak. And a conical member having sealing means located at a spaced position from said narrowed vertex. 18. The liner according to claim 17, wherein the conical member comprises a first end comprising a narrowed vertex and a second end spaced adjacent to the body, wherein the sealing means is adjacent or positioned to the second end. 19. The liner of claim 18, wherein the sealing means comprises an O-ring surrounding the conical member. 20. The liner according to claim 19, wherein the sealing means comprises an O-ring integral with the conical member. 21. The liner of claim 20, comprising an O-ring linearly integrated with the body. 22. The liner of claim 21 having a size to fit within the reaction vessel of the cassette. The liner of claim 22 wherein the liner is acrylic. Six conical members integral with one body, each conical member having a aperture at a narrowed peak, having a first end spaced from the body and an O-ring that is integrally wrapped with the conical member; A liner comprising a second end spaced from the first end. A foil sealed cassette comprising six reaction vessels linearly arranged therein and six conical members linearly dependent on the body, wherein the conical member includes narrowed vertices capable of piercing the foil sealing body; And a foil sealed cassette, wherein the liner is frictionally coupled to the cassette to seal the bond between the cassette and the liner upon insertion. 26. The foil sealed cassette of claim 25, wherein the means for sealing the bond is a zero ring that is integral with the respective conical members to enclose the respective conical members. ※ Note: The disclosure is based on the initial application.