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KR960705929A - 신규한 Fas 단백질 및 그것의 사용방법(NOVEL FAS PROTEIN AND METHODS OF USE THEREOF) - Google Patents

신규한 Fas 단백질 및 그것의 사용방법(NOVEL FAS PROTEIN AND METHODS OF USE THEREOF)

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Publication number
KR960705929A
KR960705929A KR1019960702558A KR19960702558A KR960705929A KR 960705929 A KR960705929 A KR 960705929A KR 1019960702558 A KR1019960702558 A KR 1019960702558A KR 19960702558 A KR19960702558 A KR 19960702558A KR 960705929 A KR960705929 A KR 960705929A
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South Korea
Prior art keywords
fasδtm
protein
recombinant dna
fas
biological sample
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KR1019960702558A
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English (en)
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제이. 바르 필립
피. 샤피로 죤
씨. 키퍼 마이클
Original Assignee
데이비드 엘. 토메이
엘엑스알 바이오테크놀로지 인코포레이티드
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Application filed by 데이비드 엘. 토메이, 엘엑스알 바이오테크놀로지 인코포레이티드 filed Critical 데이비드 엘. 토메이
Publication of KR960705929A publication Critical patent/KR960705929A/ko

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Abstract

본 발명은 신규한 형태의 Fas 단백질, 재조합 DNA를 발현하는 단백질 세포를 암호화하는 DNA 및 그것의 사용방법을 제공한다.

Description

신규한 Fas 단백질 및 그것의 사용방법(NOVEL FAS PROTEIN AND METHODS OF USE THEREOR)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1도는 경막(TM)영역 부근에 Fas게놈 DNA구조를 도시하는 계통도이고, 제 2도는 Fas 및 Fas△TM mRNA를 생성하기 위한 Fas RNA의 다른 스플라이싱을 도시하는 계통도이고, 제 3도는 Fas△TM의 뉴클레오티드 및 아미노산 잔기를 나타내며, 제 5도는 Fas△TM 또는 콘트롤을 발현하는 백터로 형질전환된 여러 가지 세포주, Fas항체에 노출시 비형질전환된 세포주 생존율을 나타내며, 제 7도는 Fas△TM 또는 콘트롤을 발현하는 벡터로 형질전환된 여러 가지 세포주, 비형질 전환된 세포주의 표면상의 Fas량을 나타낸다.

Claims (28)

  1. Fas△TM을 암호화하는 정제된 뉴클레오티드 서열로 이루어지는 것을 특징으로 하는 조성물.
  2. 제 1항에 있어서, 상기 서열이 cDNA인 것을 특징으로 하는 뉴클레오티드 서열.
  3. 제 1항에 있어서, 제 3도에 도시된 뉴클레오티드 서열을 갖는 것을 특징으로 하는 뉴클레오티드 서열.
  4. 제 1항의 뉴클레오티드 서열로 이루어지는 것을 특징으로 하는 재조합 DNA 벡터.
  5. 제 4항에 있어서, Fas△TM을 암호화하는 서열의 발현은 유도성 프로모터의 콘트롤하에 이루어지는 것을 특징으로 하는 재조합 DNA 벡터.
  6. 제 4항에 있어서, pBlue BAC Ⅲ-Fas△TM-3, pBluescript-Fas△TM, Fas△TM-1 및 Fas△TM-7로 구성된 군으로부터 선택되는 것을 특징으로 하는 재조합 DNA 벡터.
  7. 제 4항의 재조합 DNA 벡터로 트랜스펙션되는 것을 특징으로 하는 세포.
  8. 제 4항의 재조합 DNA 벡터로 이루어지는 것을 특징으로 하는 유전자이식 동물.
  9. 제 4도의 아미노산 서열로 이루어지는 것을 특징으로 하는 실질적으로 정제된 단백질.
  10. Fas아미노산 서열로 이루어지지만 막 결합되지 않는 분리된 단백질을 가져오는데 충분한 경막영역의 부분이 결여된 것을 특징으로 하는 실질적으로 정제된 단백질.
  11. 제 10항에 있어서, 단백질이 분비되는 것을 특징으로 하는 단백질.
  12. 제 10항에 있어서, 단백질이 재조합 DNA에 의해 발현되는 것을 특징으로 하는 단백질.
  13. 제 10항에 있어서, 단백질이 천연 단백질인 것을 특징으로 하는 단백질.
  14. Fas△TM은 인식하지남 Fas는 인식하지 않는 것을 특징으로 하는 항체.
  15. 제 14항에 있어서, 단클론성 및 다클론성으로 구성된 군으로부터 선택되는 것을 특징으로 하는 항체.
  16. 실질적으로 정제된 Fas△TM과 생리학적으로 허용되는 완충액으로 이루어지는 것을 특징으로 하는 항체.
  17. a) 생물학적 샘플을 얻는 단계; b) 항-Fas 항체를 생물학적 샘플에 첨가하는 단계; c) 항-Fas 항체가 Fas△TM과 복합체를 형성하도록 하는 조건하에서 생물학적 샘플을 유지하는 단계; 및 d) 형성된 복합체를 검출하는 단계로 이루어지는 것을 특징으로 하는 생물학적 샘플내 Fas△TM의 존재를 검출하는 방법.
  18. 제 17항에 있어서, 생물학적 샘플은 형청인 것을 특징으로 하는 방법.
  19. 제 17항에 있어서, 항-Fas 항체는 Fas△TM에 특이적인 것을 특징으로 하는 방법.
  20. Fas△TM을 암호화하는 mRNA의 생물학적 샘플내 존재를 동정하는 단계로 이루어지는 것윽 특징으로 하는 생물학적 샘플내 Fas△TM을 암호화하는 DNA의 발현을 검출하는 방법.
  21. 제 20항에 있어서, Fas△TM mRNA를 동정하는 방법은 폴리머라제 연쇄방응이고 프라이머는 경막영역을 암호화하는 mRNA에 측면위치되는 것을 특징으로 하는 방법.
  22. 제 20항에 있어서, Fas△TM mRNA를 동정하는 방법은 노던 블로팅인 것을 특징으로 하는 방법.
  23. 치료적인 효과적인 양의 Fas△TM을 환자에게 투여하는 것으로 이루어지는 것을 특징으로 하는 환자의 Fas -매개된 세포사멸을 치료하는 방법.
  24. Fas△TM의 내생적 농도를 증가시키는 단계로 이루어지는 것을 특징으로 하는 Fas-매개된 세포사멸로 부터 세포를 보호하는 방법.
  25. 제 24항에 있어서, Fas△TM은 외생적은 것을 특징으로 하는 방법.
  26. 제 24항에 있어서, Fas△TM은 세포에 의해 발현되는 것을 특징으로 하는 방법.
  27. 제 26항에 있어서, Fas△TM은 재조합 유전자에 의해 발현되는 것을 특징으로 하는 방법.
  28. 제 27항에 있어서, 유전자의 발현은 유도성 프로모터의 콘트롤하에 이루어지는 것을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019960702558A 1993-11-15 1994-11-15 신규한 Fas 단백질 및 그것의 사용방법(NOVEL FAS PROTEIN AND METHODS OF USE THEREOF) KR960705929A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/152,443 1993-11-15
US08/152,443 US5663070A (en) 1993-11-15 1993-11-15 Recombinant production of a soluble splice variant of the Fas (Apo-1) antigen, fas TM
PCT/US1994/013173 WO1995013701A1 (en) 1993-11-15 1994-11-15 Novel fas protein and methods of use thereof

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KR960705929A true KR960705929A (ko) 1996-11-08

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US (2) US5663070A (ko)
EP (1) EP0729300A4 (ko)
JP (1) JPH09510864A (ko)
KR (1) KR960705929A (ko)
AU (1) AU1056995A (ko)
CA (1) CA2176575A1 (ko)
WO (1) WO1995013701A1 (ko)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5830469A (en) * 1993-10-14 1998-11-03 Immunex Corporation Fas antagonists and uses thereof
US5888764A (en) * 1995-01-20 1999-03-30 Uab Research Foundation Human fas gene promoter region
AU3461797A (en) 1996-07-19 1998-02-10 Takeda Chemical Industries Ltd. Fas ligand-like protein, its production and use
PT932678E (pt) * 1996-09-24 2006-07-31 Tanox Inc Familia de genes que codificam peptidos relacionados com apoptose, peptidos por eles codificados e metodos para sua utilizacao
US20070299005A1 (en) 1996-09-24 2007-12-27 Tanox, Inc. Family of genes encoding apoptosis-related peptides, peptides encoded thereby and methods of use thereof
ATE273713T1 (de) * 1996-10-31 2004-09-15 Mochida Pharm Co Ltd Fas-antagonist für die prophylaxe oder therapie von gvhd
US7285267B2 (en) * 1997-01-14 2007-10-23 Human Genome Sciences, Inc. Tumor necrosis factor receptors 6α & 6β
US20040013664A1 (en) * 1997-01-14 2004-01-22 Gentz Reiner L. Tumor necrosis factor receptors 6 alpha & 6 beta
CN1247567A (zh) 1997-01-14 2000-03-15 人体基因组科学有限公司 肿瘤坏死因子受体6α和6β
US7808479B1 (en) 2003-09-02 2010-10-05 Apple Inc. Ambidextrous mouse
WO1999040196A1 (en) * 1998-02-09 1999-08-12 Genentech, Inc. Novel tumor necrosis factor receptor homolog and nucleic acids encoding the same
US6846637B1 (en) 1998-06-18 2005-01-25 Imed Ab Fas peptides and antibodies for modulating apoptosis
US6942981B1 (en) * 1999-05-14 2005-09-13 Arbor Vita Corporation Method of determining interactions with PDZ-domain polypeptides
US20050037969A1 (en) * 1999-05-14 2005-02-17 Arbor Vita Corporation Molecular interactions in hematopoietic cells
CA2389691A1 (en) * 2000-04-07 2001-10-18 Shionogi & Co., Ltd. A pre-incubation method for an assay of an inhibitor to an hcv rna-dependent rna polymerase
US11275405B2 (en) 2005-03-04 2022-03-15 Apple Inc. Multi-functional hand-held device
US7656393B2 (en) 2005-03-04 2010-02-02 Apple Inc. Electronic device having display and surrounding touch sensitive bezel for user interface and control
JP4578792B2 (ja) * 2003-09-26 2010-11-10 富士通セミコンダクター株式会社 固体撮像装置
EP2406636B1 (en) 2008-12-10 2018-11-07 Joslin Diabetes Center, Inc. Methods of diagnosing and predicting renal disease

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DE69233441T2 (de) * 1991-04-26 2005-10-13 Osaka Bioscience Institute, Suita Menschliches Zelloberflächen-Antigen codierende DNA
AU2515992A (en) * 1991-08-20 1993-03-16 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
WO1994008454A1 (en) * 1992-10-14 1994-04-28 Uab Research Foundation Methods, compositions and screening assays relating to autoimmune disease
IL111125A0 (en) * 1994-05-11 1994-12-29 Yeda Res & Dev Soluble oligomeric tnf/ngf super family ligand receptors and their use
JPH0875745A (ja) * 1994-07-06 1996-03-22 Igaku Seibutsugaku Kenkyusho:Kk 可溶性Ｆａｓ抗原の免疫学的測定方法及びその測定用キット

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