[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

KR960705209A - 녹색 형광 단백의 용도 - Google Patents

녹색 형광 단백의 용도

Info

Publication number
KR960705209A
KR960705209A KR1019960701205A KR19960701205A KR960705209A KR 960705209 A KR960705209 A KR 960705209A KR 1019960701205 A KR1019960701205 A KR 1019960701205A KR 19960701205 A KR19960701205 A KR 19960701205A KR 960705209 A KR960705209 A KR 960705209A
Authority
KR
South Korea
Prior art keywords
cell
protein
green fluorescent
cells
fluorescent protein
Prior art date
Application number
KR1019960701205A
Other languages
English (en)
Inventor
마틴 찰피
Original Assignee
잭 엠. 그랜노위츠
더 트러스티스 오브 콜롬비아 유니버시티 인 더 시티 오브 뉴욕
원본미기재
우드즈 홀 오션오그래픽 인스티튜션
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/119,678 external-priority patent/US5491084A/en
Application filed by 잭 엠. 그랜노위츠, 더 트러스티스 오브 콜롬비아 유니버시티 인 더 시티 오브 뉴욕, 원본미기재, 우드즈 홀 오션오그래픽 인스티튜션 filed Critical 잭 엠. 그랜노위츠
Publication of KR960705209A publication Critical patent/KR960705209A/ko

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Luminescent Compositions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 녹색 형광 단백질을 코딩하는 DNA 서열에 작용가능하게 연결된, 녹색 형광 단백질을 코딩하는 유전자 이외의 유전자로부터의 조절요소를 가진 DNA 분자를 포함하는 세포를 제공한다. 본 발명은 또한 상기 세포를 포함하는 생물체를 제공한다.
본 발명은 관심있는 단백질을 발현하는 세포를 선택하는 방법을 제공한다. 이 방법은 a) 관심있는 단백질을 코딩하는 DNA 서열을 가진 DNA I 분자와 녹색 형광 단백질을 코딩하는 DNA 서열을 가진 DNAⅡ 분자를 세포내에 도입하고; b) 도입된 세포를 관심있는 단백질 및 녹색 형광 단백질의 발현을 허용하는 조건하에서 배양하고; 그리고 c) 녹색 형광 단백질을 발현하는 배양된 세포를 선택함으로써, 관심있는 단백질을 발현하는 세포를 선택하는 것을 포함하는 단계를 포함한다. 마지막으로, 본 발명은 녹색 형광 단백질의 다양한 용도를 제공한다.

Description

녹색 형광 단백의 용도
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (36)

  1. 녹색 형광 단백질을 코딩하는 DNA 서열에 작용가능하게 연결된, 녹색 형광 단백질을 코딩하는 유전자 이외의유전자로부터의 조절요소를 가진 DNA 분자를 포함하는 세포.
  2. 제1항에 있어서, 사기 세포가 실질상 세균세포, 효모세포, 진균세포, 곤충세포, 선충세포, 식물 또는 동물세포로 이루어지는 군으로부터 선택되는 것을 특징으로 하는 세포.
  3. 제1항에 있어서, 상기 조절요소가 프로모터인 것을 특징으로 하는 세포.
  4. 제3항에 있어서, 상기 프로모터가 중금속에 의해 활성화되는 것을 특징으로 하는 세포.
  5. 제3항에 있어서, 상기 프로모터가 P450 유전자로부터 유래하는 것을 특징으로 하는 세포.
  6. 제3항에 있어서, 상기 프로모터가 스트레스 단백질을 코딩하는 유전자로부터 유래함을 특징으로 하는 세포.
  7. 제6항에 있어서, 상기 스트레스 단백질이 열-쇼크 단백질인 것을 특징으로 하는 세포.
  8. 제3항에 있어서, 상기 프로모터가 세포 생존에 필요한 유전자로부터 유래하는 것을 특징으로 하는 세포.
  9. 제1항에 있어서, 상기 조절요소가 인핸서인 것을 특징으로 하는 세포.
  10. 제1항에 있어서, 상기 DNA 서열이 Aequorea Victoria 녹색 형광 단백질을 코딩하는 것을 특징으로 하는 세포.
  11. 제1항에 있어서, 상기 세포가 E. coil 세포인 것을 특징으로 하는 세포.
  12. 제11항에 있어서, 상기 E. coil 세포가 SMC1(ATCC 수탁번호 제69554호)로 명명된 것임을 특징으로 하는 세포.
  13. 제1항의 세포로부터 분리된 녹색 형광 단백질.
  14. 제1항의 세포를 포함하는 생물체.
  15. 제14항에 있어서, 상기 생물체가 Caenorhabditis elegans 인 것을 특징으로 하는 생명체.
  16. 제15항에 있어서, 상기 조절요소가 스트레스 단백질에 대한 것임을 특징으로 하는 생물체.
  17. 제16항에 있어서, 상기 스트레스 단백질이 열-쇼크 단백질인 것을 특징으로 하는 생물체.
  18. a) 제1항의 세포를 배양하고; 그리고 b) 상기 세포로부터 생성된 녹색 형광 단백질을 분리 및 정제하는 것을 포함하는 녹색 형광 단백질을 생산하는 방법.
  19. 제18항에 있어서, 상기 세포가 E. coil 세포인 것을 특징으로 하는 방법.
  20. 제19항에 있어서, 상기 세포가 호기적으로 배양되는 것을 특징으로 하는 방법.
  21. 제18항에 있어서, 상기 세포가 SMC1(ATCC 수탁번호 제69554호로 명명된 것임을 특징으로 하는 세포.
  22. a) 관심있는 단백질을 코딩하는 DNA 서열을 가진 DNA I 분자의 녹색 형광 단백질을 코딩하는 DNA 서열을 가진 DNAⅡ 분자를 세포내에 도입하고; b) 도입된 세포를 관심있는 단백질 및 녹색 형광 단백질의 발현을 허용하는 조건하에서 배양하고; 그리고 c) 녹색 형광 단백질을 발현하는 배양된 세포를 선택함으로써, 관심있는 단백질을 발현하는 세포를 선택하는 것을 포함하는, 관심있는 단백질을 발현하는 세포를 선택하는 방법.
  23. 제22항에 있어서, DNA I과 DNAⅡ가 연결된 것을 특징으로 하는 방법.
  24. 제22항에 있어서, 상기 세포가 실질상 세균세포, 효모세포, 진균세포, 곤충세포, 선충세포, 식물 또는 동물세포로 이루어지는 군으로부터 선택되는 것을 특징으로 하는 방법.
  25. 제22항에 있어서, 상기 DNAⅡ가 Aequorea Victoria 녹색 형광 단백질을 암호화하는 것을 특징으로 하는 방법.
  26. a) 녹색 형광 단백질을 코딩하는 DNA 서열에 연결된 관심있는 단백질을 코딩하는 DNA 서열을 가진 DNA 분자를 이 DNA 분자에 의해 생산되는 단백질이 녹색 형광 단백질에 융합된 관심있는 단백질을 갖도록 세포내로 도입하고; b) 융합된 단백질의 발현을 허용하는 조건하에서 세포를 배양하고; 그리고 c) 상기 세포내에 녹색 형광 단백질로 이루어진 융합된 단백질의 위치를 탐지함으로써, 세포내에 관심있는 단백질의 위치를 확인하는 단계를 포함하는, 세포내에서 관심있는 단백질의 위치 확인 방법.
  27. 제26항에 있어서, 상기 세포가 관심있는 단백질을 정상적으로 발현하는 것을 특징으로 하는 방법.
  28. 제26항에 있어서, 상기 DNA가 Aequorea Victoria 녹색 형광 단백질을 코딩하는 것을 특징으로 하는 방법.
  29. 하기 단계들을 포함하는 세포내에서 우전자의 발현을탐지하는 방법: a) 녹색 형광 단백질을 코딩하는 DNA 서열에 연결된 상기 유전자의 DNA 서열을 가진 DNA 분자를 상기 유전자의 조절요소가 녹색 형광 단백질의 발현을 제어하도록 세포내에 도입하고; b) 상기 세포를 사기 유전자의 발현을 허용하는 조건하에서 배양하고; 그리고 c) 상기 세포내에서 녹색 형광 단백질의 발현을 탐지함으로써, 상기 세포내에서 상기 유전자의 발현을 보이는 단계.
  30. 하기 단계들을 포함하는 피검체에서 유전자의 발현을 탐지하는 방법: a) 녹색 형광 단백질을 코딩하는 DNA 서열에 연결된 상기 유전자의 DNA 서열을 가진 DNA 분자를 상기 우전자의 조절요소가 녹색 형광 단백질의 발현을 제어하도록 DNA 분자를 피검체의 세포내에 도입하고: b) 상기 융합된 단백질의 발현을 허용하는 조건하에서 상기 세포를 배양하고; 그리고 c) 상기 세포내에서 녹색 형광 단백질의 발현을 탐지함으로써, 상기 세포내에서 상기 유전자의 발현을 지시하는 단계.
  31. 제29항에 또는 제30항에 있어서, 상기 DNA가 Aequorea Victoria 녹색 형광 단백질을 코딩하는 것을 특징으로 하는 방법.
  32. 하기 단계들을 포함하는, 피검체에서 DNA 서열의전사의 조직-특이성을 결정하는 방법: a) 녹색 형광 단백질을 코딩하는 DNA 서열에 연결된 DNA 서열을 가진 DNA 분자를 피검체내에서 상기 DNA 서열 유전자가 녹색 형광 단백질의 발현을 제어하도록 세포내에 도입하고; b) 녹색 형광 단백질의 발현을 허용하는 조건하에서 피검체를 배양하고; 그리고 c) 피검체의 상인한 조직들에서 녹색 형광 단백질의 발현을 탐지함으로써, 상기 DNA 서열의 조직-특이성을 결정하는 단계.
  33. a) 용액내에서 제4항 또는 제5항의 세포를 배양하고; 그리고 b) 중금속의 존재를 나타내는 녹색 형광 단백질의 발현을 탐지하는 단계를 포함하는 용액내에서 중금속을 탐지하는 방법.
  34. a) 용액내에서제4,5,6 또는 7항의 세포를 배양하고; 그리고 b) 오염물질의 존재를 나타내는 녹색 단백질의 발현을 탐지하는 단계를 포함하는 용액내에서 오염물질을 탐지하는 방법.
  35. a) 녹색 형광 단백질을 코딩하는 DNA 분자를 같은 해독틀로 기지 아미노산 서열을 암호화하는 DNA 분자와 연결하고; b) 단계 a)의 연결된 DNA 분자에 의해 코딩되는 형광 단백질의 발현을 허용하는 발현 시스템에 연결된 DNA 분자를 도입하고; 그리고 c) 단계 b)의 발현된 형광 단백질의 분량을 결정함으로써, 형광 단백질 분자량 마커를 생산하는 단계.
  36. 제35항에 있어서, 상기의 발현된 단백질의 정체를 추가로 포함하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019960701205A 1993-09-10 1994-09-09 녹색 형광 단백의 용도 KR960705209A (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/119,678 1993-09-10
US08/119,678 US5491084A (en) 1993-09-10 1993-09-10 Uses of green-fluorescent protein
US19227494A 1994-02-04 1994-02-04
US08/192,274 1994-02-04
PCT/US1994/010165 WO1995007463A1 (en) 1993-09-10 1994-09-09 Uses of green fluorescent protein

Publications (1)

Publication Number Publication Date
KR960705209A true KR960705209A (ko) 1996-10-09

Family

ID=26817579

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019960701205A KR960705209A (ko) 1993-09-10 1994-09-09 녹색 형광 단백의 용도

Country Status (9)

Country Link
US (1) US6146826A (ko)
EP (1) EP0759170B1 (ko)
JP (1) JP3810791B2 (ko)
KR (1) KR960705209A (ko)
AT (1) ATE400651T1 (ko)
AU (1) AU694745B2 (ko)
CA (1) CA2169298A1 (ko)
DE (1) DE69435112D1 (ko)
WO (1) WO1995007463A1 (ko)

Families Citing this family (245)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795737A (en) * 1994-09-19 1998-08-18 The General Hospital Corporation High level expression of proteins
US5625048A (en) 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US5777079A (en) * 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
DE69635355T2 (de) * 1995-01-31 2006-08-03 Bioimage A/S Verfahren zum Nachweis von biologisch aktiven Substanzen
US5958713A (en) * 1995-01-31 1999-09-28 Novo Nordisk A/S Method of detecting biologically active substances by using green fluorescent protein
DK0815257T3 (da) * 1995-01-31 2002-04-22 Bioimage As Fremgangsmåde til karakterisering af biologisk aktive stoffer
GB9504446D0 (en) * 1995-03-06 1995-04-26 Medical Res Council Improvements in or relating to gene expression
AU4482996A (en) * 1995-09-22 1997-04-09 Novo Nordisk A/S Novel variants of green fluorescent protein, gfp
AU1283497A (en) 1995-12-08 1997-06-27 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Method and compositions for monitoring dna binding molecules in living cells
US5874304A (en) * 1996-01-18 1999-02-23 University Of Florida Research Foundation, Inc. Humanized green fluorescent protein genes and methods
US6803188B1 (en) 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US6900304B2 (en) 1996-01-31 2005-05-31 The Regents Of The University Of California Emission ratiometric indicators of phosphorylation
US6077697A (en) 1996-04-10 2000-06-20 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
CA2250966A1 (en) * 1996-04-19 1997-10-30 Mycogen Plant Science, Inc. Monitoring of transgenic plants with in vivo markers
AR006928A1 (es) 1996-05-01 1999-09-29 Pioneer Hi Bred Int Una molecula de adn aislada que codifica una proteina fluorescente verde como marcador rastreable para la transformacion de plantas, un metodo para laproduccion de plantas transgenicas, un vector de expresion, una planta transgenica y celulas de dichas plantas.
US6096865A (en) * 1996-05-06 2000-08-01 Amgen Inc. Mutants of the green fluorescent protein having improved fluorescent properties at 37°
US6027881A (en) * 1996-05-08 2000-02-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Mutant Aequorea victoria fluorescent proteins having increased cellular fluorescence
US5989835A (en) 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
US5876711A (en) * 1996-05-31 1999-03-02 Onyx Pharmaceuticals, Inc. Methods and compositions for determining the tumor suppressor status of cells
US5912137A (en) 1996-07-16 1999-06-15 The Regents Of The University Of California Assays for protein kinases using fluorescent
US5925558A (en) * 1996-07-16 1999-07-20 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
US6124128A (en) 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
US6593135B2 (en) 1996-08-16 2003-07-15 The State Of Oregon, Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Long wavelength engineered fluorescent proteins
WO1998021355A1 (en) 1996-11-15 1998-05-22 Life Technologies, Inc. Mutants of green fluorescent protein
US6245564B1 (en) * 1997-01-23 2001-06-12 Cornell Research Foundation, Inc. Method for separating cells
US7117098B1 (en) 1997-02-27 2006-10-03 Cellomics, Inc. Machine-readable storage medium for analyzing distribution of macromolecules between the cell membrane and the cell cytoplasm
US6727071B1 (en) 1997-02-27 2004-04-27 Cellomics, Inc. System for cell-based screening
AU730100B2 (en) 1997-02-27 2001-02-22 Cellomics, Inc. A system for cell-based screening
US5998204A (en) * 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
GB9706414D0 (en) * 1997-03-27 1997-05-14 Univ Manchester Detection of DNA damage
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
AU6820998A (en) * 1997-04-07 1998-10-30 Bioimage A/S A method for extracting quantitative information relating to an influence on a cellular response
US6046925A (en) * 1997-04-14 2000-04-04 The Regents Of The University Of California Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins
DE19715683C2 (de) * 1997-04-15 1999-11-11 Max Planck Gesellschaft Neue Reportervektoren für One-Hybrid und Two-Hybrid Systeme
AU9161998A (en) * 1997-07-29 1999-02-22 Unilever Plc Plasmids
GB2328209A (en) * 1997-08-16 1999-02-17 Stefan Andreas Oehler Vectors which express a coloured protein for detection of integration into a cell
WO1999037142A1 (en) * 1998-01-27 1999-07-29 Novo Nordisk A/S Method for producing transgenic animals
EP1555317B1 (en) 1998-03-20 2011-09-28 Commonwealth Scientific And Industrial Research Organisation Synthetic genes and genetic constructs comprising the same
EP1925320A3 (en) 1998-03-27 2008-09-03 Prolume, Ltd. Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics
US7090976B2 (en) 1999-11-10 2006-08-15 Rigel Pharmaceuticals, Inc. Methods and compositions comprising Renilla GFP
EP1121593A2 (en) * 1998-10-15 2001-08-08 BioImage A/S Method for extracting quantitative information relating to an influence on a cellular response
EP1031630B1 (en) * 1999-02-22 2004-10-20 Matsushita Electric Industrial Co., Ltd. Method for detecting bacteria
US8148110B2 (en) 1999-03-15 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by β-lactamase reporter fragment complementation
WO2001009177A2 (en) * 1999-07-29 2001-02-08 Whitehead Institute For Biomedical Research Affinity fluorescent proteins and uses thereof
US6716614B1 (en) 1999-09-02 2004-04-06 Lexicon Genetics Incorporated Human calcium dependent proteases, polynucleotides encoding the same, and uses thereof
US6541252B1 (en) 2000-05-19 2003-04-01 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
EP1228233B1 (en) * 1999-11-10 2007-01-10 Rigel Pharmaceuticals, Inc. Methods and compositions comprising renilla gfp
US6716588B2 (en) 1999-12-09 2004-04-06 Cellomics, Inc. System for cell-based screening
KR100414641B1 (ko) * 2000-04-07 2004-01-13 동부한농화학 주식회사 형질전환식물체의 생체분석방법 및 그를 이용한 시스템
AU2001279669B2 (en) 2000-06-19 2006-02-02 Bioimage As Fluorescent proteins
KR100393709B1 (ko) * 2000-08-07 2003-08-06 광주과학기술원 재조합 동물 세포주를 이용한 환경 오염 물질의 탐지 방법
JP2004533221A (ja) * 2000-10-27 2004-11-04 レキシコン・ジェネティクス・インコーポレーテッド 新規ヒト７ｔｍタンパクおよびそれをコードするポリヌクレオチド
EP1330524A2 (en) * 2000-10-30 2003-07-30 Lexicon Genetics Incorporated Novel human 7tm proteins and polynucleotides encoding the same
DE10136702B4 (de) 2000-12-27 2004-04-22 Axiogenesis Ag System zur zell- und entwicklungsspezifischen Selektion differenzierender embryonaler Stammzellen, adulter Stammzellen und embryonaler Keimbahnzellen
EP1236738A1 (en) * 2001-02-23 2002-09-04 Jungbauer, Alois, Professor Dr. Internal standard for electrophoretic and chromatographic separation process
JP3929250B2 (ja) * 2001-03-08 2007-06-13 株式会社ルネサステクノロジ 半導体装置
CA2440592C (en) * 2001-03-12 2012-05-15 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Oxidation-reduction sensitive green fluorescent protein variants
US20020188965A1 (en) 2001-04-20 2002-12-12 Zou-Yu Zhao Methods of transforming plants
US20050287647A9 (en) 2001-05-30 2005-12-29 Carl Perez Plant artificial chromosomes, uses thereof and methods of preparing plant artificial chromosomes
JP3829252B2 (ja) 2001-06-08 2006-10-04 独立行政法人理化学研究所 蛍光蛋白質
US6894205B2 (en) * 2001-06-22 2005-05-17 Vanderbilt University Assay for toxin induced neuronal degeneration and viability in C. elegans
US7393656B2 (en) 2001-07-10 2008-07-01 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for risk stratification
US8048644B1 (en) 2001-08-01 2011-11-01 The University Of North Carolina At Charlotte Biological indicator for monitoring the transport of micro-organisms in the environment
AU2002327433A1 (en) 2001-08-10 2003-02-24 Cornell Research Foundation, Inc. Telomerase immortalized human neutral stem cells and phenotypically-restricted progenitor cells
CN1582332A (zh) * 2001-12-29 2005-02-16 Seoul大学校产学协力财团 转染gfp的克隆猪、敲除gt的克隆猪及其生产方法
AU2003210959A1 (en) * 2002-02-12 2003-09-04 Cornell Research Foundation, Inc. Identification and high-yield isolation of human pancreatic islet progenitor and stem cells
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
DE10256083A1 (de) * 2002-11-29 2004-08-12 Boehringer Ingelheim Pharma Gmbh & Co. Kg Expressionsvektor, Verfahren zur Herstellung von heterologen Genprodukten und Selektionsverfahren für hochproduzierende rekombinante Zellen
US7384744B2 (en) * 2002-11-29 2008-06-10 Boehringer Ingelheim Pharma Gmbh & Co., Kg Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products
JP4904153B2 (ja) 2003-06-20 2012-03-28 アキシオジェネシス エージー 胚性幹（ｅｓ）細胞系での組織モデリング
PT2343364T (pt) 2003-08-25 2017-01-02 Monsanto Technology Llc Elementos regulatórios de tubulina para utilização em plantas
US8399618B2 (en) 2004-10-21 2013-03-19 Xencor, Inc. Immunoglobulin insertions, deletions, and substitutions
US8883147B2 (en) 2004-10-21 2014-11-11 Xencor, Inc. Immunoglobulins insertions, deletions, and substitutions
CA2541970A1 (en) 2003-10-09 2005-06-09 E.I. Du Pont De Nemours And Company Gene silencing by using micro-rna molecules
US7279275B2 (en) 2003-11-03 2007-10-09 Washington University Methods and compositions for detection of segmented negative strand RNA viruses
EP1718663B1 (en) 2004-01-20 2011-07-13 Monsanto Technology, LLC Chimeric promoters for use in plants
US7481997B1 (en) 2004-02-12 2009-01-27 Montana State University Snow mountain virus genome sequence, virus-like particles and methods of use
CA2560334A1 (en) 2004-04-07 2005-10-20 Axiogenesis Ag Non-invasive, in vitro functional tissue assay systems
ATE490306T1 (de) 2004-05-11 2010-12-15 Axiogenesis Ag Methoden zur medikamentenaufspürung auf der basis von in vitro differenzierten zellen
US8603824B2 (en) 2004-07-26 2013-12-10 Pfenex, Inc. Process for improved protein expression by strain engineering
WO2006016238A2 (en) * 2004-08-04 2006-02-16 Applied Research Systems Ars Holding N.V. Capsules containing transfected cells, method for preparing the same and uses thereof for immunization and vaccination
US8802820B2 (en) 2004-11-12 2014-08-12 Xencor, Inc. Fc variants with altered binding to FcRn
EP2845865A1 (en) 2004-11-12 2015-03-11 Xencor Inc. Fc variants with altered binding to FcRn
DK1888640T3 (da) 2005-05-18 2012-06-18 Ablynx Nv Forbedrede nanobodies mod tumornekrosefaktor-alfa
RU2433139C2 (ru) 2005-05-20 2011-11-10 Аблинкс Н.В. Nanobodies tm для лечения заболеваний, опосредованных агрегацией
US8362250B2 (en) 2005-05-24 2013-01-29 Enzo Biochem, Inc. Fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest
AU2006292827B2 (en) 2005-08-09 2013-02-14 Revivicor, Inc. Transgenic ungulates expressing CTLA4-IG and uses thereof
US8859846B2 (en) 2005-09-21 2014-10-14 E. I. Du Pont De Nemours And Company Doubling of chromosomes in haploid embryos
WO2007052102A2 (en) 2005-11-04 2007-05-10 Evrogen, Jsc Modified green fluorescent proteins and methods for using same
US8298818B2 (en) 2006-04-28 2012-10-30 University Of Florida Research Foundation, Inc. Self-complementary adeno-associated virus having a truncated CMV-chicken β-actin promoter
AU2007257925B2 (en) 2006-06-06 2012-07-05 Monsanto Technology Llc Method for selection of transformed cells
US7855326B2 (en) * 2006-06-06 2010-12-21 Monsanto Technology Llc Methods for weed control using plants having dicamba-degrading enzymatic activity
US20080004410A1 (en) * 2006-06-30 2008-01-03 Yu-Chin Lai Hydrophilic macromonomers having alpha,beta-conjugated carboxylic terminal group and medical devices incorporating same
EP2442109A1 (en) 2006-07-14 2012-04-18 The Regents of the University of California Cancer biomarkers and methods of use thereof
US7767206B2 (en) 2006-10-02 2010-08-03 Amgen Inc. Neutralizing determinants of IL-17 Receptor A and antibodies that bind thereto
EP2121938A2 (en) 2006-10-05 2009-11-25 E.I. Du Pont De Nemours And Company Maize microrna sequences
CA2672965C (en) 2006-12-19 2018-02-06 Ablynx N.V. Amino acid sequences directed against a metalloproteinase from the adam family and polypeptides comprising the same for the treatment of adam-related diseases and disorders
WO2008074839A2 (en) 2006-12-19 2008-06-26 Ablynx N.V. Amino acid sequences directed against gpcrs and polypeptides comprising the same for the treatment of gpcr-related diseases and disorders
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
KR101530395B1 (ko) 2007-04-27 2015-06-19 피페넥스 인크. 향상된 수율 및(또는) 품질로 이종 단백질을 발현하는 특정 균주를 동정하기 위해 미생물 숙주를 신속히 스크리닝하는 방법
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
ES2650224T3 (es) 2007-08-21 2018-01-17 Amgen, Inc. Proteínas de unión al antígeno C-FMS humano
WO2009025847A2 (en) 2007-08-21 2009-02-26 Nodality, Inc. Methods for diagnosis, prognosis and methods of treatment
US20090081715A1 (en) * 2007-09-07 2009-03-26 Cobalt Technologies, Inc., A Delaware Corporation Engineered Light-Emitting Reporter Genes
TW200918553A (en) 2007-09-18 2009-05-01 Amgen Inc Human GM-CSF antigen binding proteins
CN103323610B (zh) 2007-10-02 2016-12-28 赛拉诺斯股份有限公司 模块化现场护理装置及其应用
US8619257B2 (en) 2007-12-13 2013-12-31 Kimberley-Clark Worldwide, Inc. Recombinant bacteriophage for detection of nosocomial infection
PT2808343T (pt) 2007-12-26 2019-09-04 Xencor Inc Variantes fc com ligação alterada a fcrn
EP2607488B1 (en) 2008-04-07 2016-11-02 Monsanto Technology LLC Plant regulatory elements and uses thereof
EP2260058A2 (en) 2008-04-07 2010-12-15 Ablynx N.V. Single variable domains against the notch pathways
CN102057046A (zh) 2008-04-09 2011-05-11 钴技术有限公司 固定的产物耐受的微生物
US9334281B2 (en) * 2008-09-08 2016-05-10 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
US9250249B2 (en) 2008-09-08 2016-02-02 Enzo Biochem, Inc. Autophagy and phospholipidosis pathway assays
US8309306B2 (en) 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
CN107011434B (zh) 2008-12-19 2021-02-19 埃博灵克斯股份有限公司 用于产生针对细胞相关抗原如p2x7、cxcr7或cxcr4的免疫球蛋白的基因免疫
JO3382B1 (ar) 2008-12-23 2019-03-13 Amgen Inc أجسام مضادة ترتبط مع مستقبل cgrp بشري
US8242248B2 (en) 2009-03-23 2012-08-14 Nodality, Inc. Kits for multiparametric phospho analysis
CN105399828B (zh) 2009-04-10 2021-01-15 埃博灵克斯股份有限公司 用于治疗il-6r相关疾病和病症的改进的针对il-6r的氨基酸序列和包含其的多肽
NZ597314A (en) 2009-06-05 2013-07-26 Ablynx Nv Monovalent, bivalent and trivalent anti human respiratory syncytial virus (hrsv) nanobody constructs for the prevention and/or treatment of respiratory tract infections
BRPI1010056A2 (pt) * 2009-06-26 2017-06-27 Cobalt Tech Inc processo para produção de bioproduto e sistema para a produção de bioproduto
TW201117824A (en) 2009-10-12 2011-06-01 Amgen Inc Use of IL-17 receptor a antigen binding proteins
EP2507262A1 (en) 2009-11-30 2012-10-10 Ablynx N.V. Improved amino acid sequences directed against human respiratory syncytial virus (hrsv) and polypeptides comprising the same for the prevention and/or treatment of respiratory tract infections
UA109888C2 (uk) 2009-12-07 2015-10-26 ІЗОЛЬОВАНЕ АНТИТІЛО АБО ЙОГО ФРАГМЕНТ, ЩО ЗВ'ЯЗУЄТЬСЯ З β-КЛОТО, РЕЦЕПТОРАМИ FGF І ЇХНІМИ КОМПЛЕКСАМИ
WO2011083140A1 (en) 2010-01-08 2011-07-14 Ablynx Nv Immunoglobulin single variable domain directed against human cxcr4
AR079909A1 (es) 2010-01-14 2012-02-29 Monsanto Technology Llc Elementos de regulacion vegetal y sus usos
CA2796055A1 (en) 2010-04-15 2011-10-20 Amgen Inc. Human fgf receptor and .beta.-klotho binding proteins
US20120003639A1 (en) 2010-04-27 2012-01-05 Prelude, Inc. Cancer biomarkers and methods of use thereof
CN103201389B (zh) 2010-08-30 2015-09-23 陶氏益农公司 甘蔗杆状病毒(scbv)增强子及其在植物功能基因组中的用途
WO2012030738A2 (en) 2010-08-30 2012-03-08 Beckman Coulter, Inc. Complex phosphoprotein activation profiles
WO2012098260A1 (en) 2011-01-21 2012-07-26 Axiogenesis Ag A non-viral system for the generation of induced pluripotent stem (ips) cells
EP4024029A3 (en) 2011-01-21 2022-09-14 Labrador Diagnostics LLC Systems and methods for sample use maximization
KR102003175B1 (ko) 2011-03-25 2019-07-24 몬산토 테크놀로지 엘엘씨 식물 조절 요소 및 그의 용도
WO2012155071A1 (en) 2011-05-12 2012-11-15 Gilead Biologics, Inc. Methods of identifying lysyl oxidase - like - 2 (loxl2) binding partners
AR086365A1 (es) 2011-05-13 2013-12-11 Monsanto Technology Llc Elementos reguladores de las plantas y sus usos
US9574002B2 (en) 2011-06-06 2017-02-21 Amgen Inc. Human antigen binding proteins that bind to a complex comprising β-Klotho and an FGF receptor
WO2013016220A1 (en) 2011-07-22 2013-01-31 Amgen Inc. Il-17 receptor a is required for il-17c biology
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US8435738B2 (en) 2011-09-25 2013-05-07 Theranos, Inc. Systems and methods for multi-analysis
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
US8840838B2 (en) 2011-09-25 2014-09-23 Theranos, Inc. Centrifuge configurations
EA201790757A1 (ru) 2011-09-22 2017-07-31 Эмджен Инк. Связывающие антиген cd27l белки
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
CN104220460A (zh) 2011-12-08 2014-12-17 安姆根有限公司 激动人lcat抗原结合蛋白和它们在疗法中的用途
CA2865977A1 (en) 2012-02-29 2013-09-06 Dow Agrosciences Llc Sugarcane bacilliform viral (scbv) enhancer and its use in plant functional genomics
US9663793B2 (en) 2012-04-20 2017-05-30 Monsanto Technology, Llc Plant regulatory elements and uses thereof
JO3623B1 (ar) 2012-05-18 2020-08-27 Amgen Inc البروتينات المرتبطة بمولد المستضاد st2
EP2859331A2 (en) 2012-06-06 2015-04-15 The United States of America, as represented by The Secretary, Department of Health and Human Services Kits for detecting and monitoring endocrine disrupting chemicals (edcs)
US9645151B2 (en) 2012-08-17 2017-05-09 California Institute Of Technology Targeting phosphofructokinase and its glycosylation form for cancer
WO2014036048A1 (en) 2012-08-30 2014-03-06 E. I. Du Pont De Nemours And Company Long intergenic non-coding rnas in maize
TW201425336A (zh) 2012-12-07 2014-07-01 Amgen Inc Ｂｃｍａ抗原結合蛋白質
US9920121B2 (en) 2013-01-25 2018-03-20 Amgen Inc. Antibodies targeting CDH19 for melanoma
JO3519B1 (ar) 2013-01-25 2020-07-05 Amgen Inc تركيبات أجسام مضادة لأجل cdh19 و cd3
US20140271629A1 (en) 2013-03-14 2014-09-18 Amgen Inc. Chrdl-1 antigen binding proteins and methods of treatment
US9850297B2 (en) 2013-03-15 2017-12-26 Amgen Inc. Secreted frizzle-related protein 5 (SFRP5) binding proteins
EP2970449B1 (en) 2013-03-15 2019-09-25 Amgen Research (Munich) GmbH Single chain binding molecules comprising n-terminal abp
TW201902931A (zh) 2013-03-15 2019-01-16 美商安美基公司 人類pac1抗體
EP2970446A1 (en) 2013-03-15 2016-01-20 Amgen Research (Munich) GmbH Antibody constructs for influenza m2 and cd3
CN105555803B (zh) 2013-05-30 2019-09-10 基尼克萨制药有限公司 制瘤素m受体抗原结合蛋白
US10227370B2 (en) 2013-08-02 2019-03-12 California Institute Of Technology Heparan sulfate/heparin mimetics with anti-chemokine and anti-inflammatory activity
US9770461B2 (en) 2013-08-02 2017-09-26 California Institute Of Technology Tailored glycopolymers as anticoagulant heparin mimetics
TW201605896A (zh) 2013-08-30 2016-02-16 安美基股份有限公司 Gitr抗原結合蛋白
TWI688401B (zh) 2013-09-13 2020-03-21 美商安進公司 用於治療骨髓性白血病的表觀遺傳因子與靶向ｃｄ３３及ｃｄ３之雙特異性化合物的組合
AU2015218903B2 (en) 2014-02-20 2020-10-15 Allergan, Inc. Complement component C5 antibodies
RU2673036C2 (ru) 2014-02-27 2018-11-21 Аллерган, Инк. АНТИТЕЛА К ФАКТОРУ КОМПЛЕМЕНТА Bb
NL2013661B1 (en) 2014-10-21 2016-10-05 Ablynx Nv KV1.3 Binding immunoglobulins.
WO2016007886A1 (en) 2014-07-11 2016-01-14 Northwestern University Yeast-based biosensor
AR101669A1 (es) 2014-07-31 2017-01-04 Amgen Res (Munich) Gmbh Constructos de anticuerpos para cdh19 y cd3
AU2015294834B2 (en) 2014-07-31 2021-04-29 Amgen Research (Munich) Gmbh Optimized cross-species specific bispecific single chain antibody constructs
MX2017001403A (es) 2014-07-31 2017-07-07 Amgen Res (Munich) Gmbh Constructo de anticuerpo de cadena individual biespecifico con distribucion mejorada de tejido.
DE102014012130B3 (de) 2014-08-13 2015-03-05 Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung Nachweisverfahren unter Verwendung von rekombinanten lebenden Zellen zum Nachweis von xenobiotischen Stoffen sowie Anordnung und Testkit zur Durchführung des Nachweisverfahrens
WO2016040767A2 (en) 2014-09-12 2016-03-17 Amgen Inc. Chrdl-1 epitopes and antibodies
US11543411B2 (en) 2014-12-05 2023-01-03 Prelude Corporation DCIS recurrence and invasive breast cancer
EP3250595B1 (en) 2015-01-29 2024-04-24 Technophage, Investigação E Desenvolvimento Em Biotecnologia, SA Antibody molecules and peptide delivery systems for use in alzheimer's disease and related disorders
WO2016126990A1 (en) 2015-02-04 2016-08-11 Monsanto Technology Llc Methods for plastid transformation
WO2016154631A1 (en) 2015-03-26 2016-09-29 The Texas A&M University System Conversion of lignin into bioplastics and lipid fuels
PT3283524T (pt) 2015-04-17 2023-06-05 Amgen Res Munich Gmbh Construções de anticorpos biespecíficos para cdh3 e cd3
WO2016189118A1 (en) 2015-05-28 2016-12-01 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods of prognosis and treatment of patients suffering from acute myeloid leukemia
TW202346349A (zh) 2015-07-31 2023-12-01 德商安美基研究（慕尼黑）公司 Dll3及cd3抗體構築體
TWI744242B (zh) 2015-07-31 2021-11-01 德商安美基研究（慕尼黑）公司 Egfrviii及cd3抗體構築體
TWI829617B (zh) 2015-07-31 2024-01-21 德商安美基研究（慕尼黑）公司 Ｆｌｔ３及ｃｄ３抗體構築體
TWI717375B (zh) 2015-07-31 2021-02-01 德商安美基研究（慕尼黑）公司 Ｃｄ７０及ｃｄ３抗體構築體
TWI796283B (zh) 2015-07-31 2023-03-21 德商安美基研究（慕尼黑）公司 Ｍｓｌｎ及ｃｄ３抗體構築體
WO2017112824A2 (en) 2015-12-23 2017-06-29 Amgen Inc. Method of treating or ameliorating metabolic disorders using binding proteins for gastric inhibitory peptide receptor (gipr) in combination with glp-1 agonists
JOP20170017B1 (ar) 2016-01-25 2021-08-17 Amgen Res Munich Gmbh تركيب صيدلي يتضمن تركيبات جسم مضاد ثنائي الاختصاص
EA039859B1 (ru) 2016-02-03 2022-03-21 Эмджен Рисерч (Мюник) Гмбх Биспецифические конструкты антител, связывающие egfrviii и cd3
DK3411402T3 (da) 2016-02-03 2022-02-07 Amgen Res Munich Gmbh Bcma- og cd3-bispecifikke t-celle-engagerende antistofkonstruktioner
CN109311979A (zh) 2016-02-03 2019-02-05 安进研发（慕尼黑）股份有限公司 Psma和cd3双特异性t细胞接合抗体构建体
JOP20170091B1 (ar) 2016-04-19 2021-08-17 Amgen Res Munich Gmbh إعطاء تركيبة ثنائية النوعية ترتبط بـ cd33 وcd3 للاستخدام في طريقة لعلاج اللوكيميا النخاعية
KR101913163B1 (ko) 2016-06-30 2018-11-01 주식회사 바이오이즈 이중가닥 핵산 신호 프로브 및 이를 이용한 표적분자의 검출방법
EP3485020A1 (en) 2016-07-18 2019-05-22 University of Bremen Method of generating polyploid plants
KR20240076829A (ko) 2016-11-16 2024-05-30 아블린쓰 엔.브이. Cd123 및 tcr 알파/베타에 결합할 수 있는 t 세포 동원 폴리펩티드
JOP20190177A1 (ar) 2017-01-17 2019-07-16 Amgen Inc طريقة لعلاج أو تحسين اضطرابات أيضية باستخدام مساعدات مستقبل glp-1 مقترنة بمناهضات لمستقبل ببتيد مثبط معوي (gipr)
JOP20190189A1 (ar) 2017-02-02 2019-08-01 Amgen Res Munich Gmbh تركيبة صيدلانية ذات درجة حموضة منخفضة تتضمن بنيات جسم مضاد يستهدف الخلية t
BR112019017001A2 (pt) 2017-02-16 2020-04-28 Sonnet Bio Therapeutics composição, proteína de fusão, ácido nucleico, célula hospedeira, e, métodos para produção de um domínio de aglutinação de albumina, para preparação de uma variante de il-15, para produção de uma proteína de fusão e para inibição ou redução de um tumor em um indivíduo em necessidade do mesmo.
EP3618864A1 (en) 2017-05-05 2020-03-11 Amgen Inc. Pharmaceutical composition comprising bispecific antibody constructs for improved storage and administration
JOP20190259A1 (ar) 2017-05-31 2019-10-31 Amgen Inc بروتينات ربط مولد ضد مضادة لـ jagged1
AR111842A1 (es) 2017-06-02 2019-08-21 Amgen Inc Antagonistas de péptido pac1
EP3642239A1 (en) 2017-06-20 2020-04-29 Amgen Inc. Method of treating or ameliorating metabolic disorders using binding proteins for gastric inhibitory peptide receptor (gipr) in combination with glp-1 agonists
CA3071852A1 (en) 2017-08-04 2019-02-07 Amgen Inc. Method of conjugation of cys-mabs
JP7344206B2 (ja) 2017-12-11 2023-09-13 アムジェン インコーポレイテッド 二重特異性抗体製品のための連続製造プロセス
TW201940518A (zh) 2017-12-29 2019-10-16 美商安進公司 針對ｍｕｃ１７和ｃｄ３之雙特異性抗體構建體
EP3737747A1 (en) 2018-01-12 2020-11-18 The Texas A&M University System Increasing plant bioproduct yield
WO2019162521A1 (en) 2018-02-26 2019-08-29 Ablynx Nv Improved nucleotide sequences encoding peptide linkers
WO2020025532A1 (en) 2018-07-30 2020-02-06 Amgen Research (Munich) Gmbh Prolonged administration of a bispecific antibody construct binding to cd33 and cd3
US11692031B2 (en) 2018-08-03 2023-07-04 Amgen Research (Munich) Gmbh Antibody constructs for CLDN18.2 and CD3
EP3850368A4 (en) 2018-09-14 2022-11-23 Prelude Corporation PROCEDURE FOR SELECTING THE TREATMENT OF SUBJECTS AT RISK FOR INVASIVE BREAST CANCER
TW202028239A (zh) 2018-09-28 2020-08-01 美商安進公司 針對可溶性bcma之抗體
SG11202103275YA (en) 2018-10-11 2021-04-29 Amgen Inc Downstream processing of bispecific antibody constructs
JP2022512860A (ja) 2018-11-06 2022-02-07 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル 白血病幹細胞を根絶することによる急性骨髄性白血病の治療のための方法および医薬組成物
WO2020142625A2 (en) 2019-01-03 2020-07-09 Invetx Inc. Compositions for increasing half-life of a therapeutic agent in canines and methods of use
CA3137494A1 (en) 2019-06-13 2020-12-17 Amgen Inc. Automated biomass-based perfusion control in the manufacturing of biologics
JP2022547135A (ja) 2019-09-10 2022-11-10 アムジエン・インコーポレーテツド 増強されたプロテインｌ捕捉動的結合容量を有する二重特異性抗原結合ポリペプチドの精製方法
CA3156683A1 (en) 2019-11-13 2021-05-20 Amgen Inc. METHOD FOR REDUCING AGGREGATE FORMATION IN DOWNSTREAM PROCESSING OF BI-SPECIFIC ANTIGEN-BINDING MOLECULES
EP4093771A1 (en) 2020-01-22 2022-11-30 Amgen Research (Munich) GmbH Combinations of antibody constructs and inhibitors of cytokine release syndrome and uses thereof
BR112022018111A2 (pt) 2020-03-19 2022-10-25 Amgen Inc Anticorpos contra mucina 17 e usos dos mesmos
CN115867572A (zh) 2020-05-11 2023-03-28 因外泰克斯公司 用于增加治疗剂在犬中的半衰期的组合物和使用方法
CA3183756A1 (en) 2020-05-19 2021-11-25 Amgen Inc. Mageb2 binding constructs
TW202210101A (zh) 2020-05-29 2022-03-16 美商安進公司 與ｃｄ３３和ｃｄ３結合的雙特異性構建體的減輕不良反應投與
WO2022008488A1 (en) 2020-07-06 2022-01-13 INSERM (Institut National de la Santé et de la Recherche Médicale) Use of slamf1 as a biomarker in colorectal cancer
MX2023000500A (es) 2020-07-10 2023-04-12 Invetx Inc Composiciones para aumentar la semivida de un agente terapeutico en felinos y modos de uso.
WO2022013256A1 (en) 2020-07-15 2022-01-20 INSERM (Institut National de la Santé et de la Recherche Médicale) Use of cd160 as a biomarker in acute myeloid leukemia
EP4240768A2 (en) 2020-11-06 2023-09-13 Amgen Inc. Multitargeting bispecific antigen-binding molecules of increased selectivity
MX2023005323A (es) 2020-11-06 2023-05-19 Amgen Inc Construcciones polipeptidicas que se unen a cd3.
KR20230098334A (ko) 2020-11-06 2023-07-03 암젠 리서치 (뮌헨) 게엠베하 Cldn6 및 cd3에 선택적으로 결합하는 폴리펩디드 작제물
JP2023548345A (ja) 2020-11-06 2023-11-16 アムジエン・インコーポレーテツド クリッピング率の低減した抗原結合ドメイン
US12103961B2 (en) 2020-12-07 2024-10-01 Invetx, Inc. Compositions for increasing half-life of a therapeutic agent in livestock animals and methods of use
US12092642B2 (en) 2021-01-20 2024-09-17 Nautilus Subsidiary, Inc. Systems and methods for biomolecule quantitation
WO2022212831A1 (en) 2021-04-02 2022-10-06 Amgen Inc. Mageb2 binding constructs
IL307672A (en) 2021-05-06 2023-12-01 Amgen Res Munich Gmbh CD20 and CD22 targeting antigen-binding molecules for use in proliferative diseases
CA3232591A1 (en) 2021-09-17 2023-03-23 University Of Freiburg Proteins for regulation of symbiotic infection and associated regulatory elements
WO2023041987A1 (en) 2021-09-20 2023-03-23 University Of Freiburg Pin6 proteins for the formation of nodule-like structures
WO2023218027A1 (en) 2022-05-12 2023-11-16 Amgen Research (Munich) Gmbh Multichain multitargeting bispecific antigen-binding molecules of increased selectivity
WO2024009146A2 (en) 2022-07-05 2024-01-11 University Of Freiburg Plant regulatory elements and uses thereof
US20240093220A1 (en) 2022-09-09 2024-03-21 Friedrich Alexander Universität Erlangen-Nürnberg Plant regulatory elements and uses thereof
WO2024059675A2 (en) 2022-09-14 2024-03-21 Amgen Inc. Bispecific molecule stabilizing composition
WO2024104551A1 (en) 2022-11-14 2024-05-23 INSERM (Institut National de la Santé et de la Recherche Médicale) Use of the population of slamf4+ ccr5+ effector memory cd4+ t cells as a biomarker in rheumatoid arthritis
US20240209096A1 (en) 2022-12-27 2024-06-27 Invetx, Inc. Polypeptides with altered binding to neonatal fc receptor (fcrn) and methods of use
WO2024145280A2 (en) 2022-12-27 2024-07-04 Invetx, Inc. Polypeptides with altered binding to neonatal fc receptor (fcrn) and methods of use
WO2024155982A2 (en) 2023-01-20 2024-07-25 Invetx, Inc. Bispecific binding agents for use in companion animals

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ214563A (en) * 1984-12-31 1991-05-28 Univ Georgia Production of apoaeqorin using recombinant dna
US5268463A (en) * 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
GB8916806D0 (en) * 1989-07-22 1989-09-06 Univ Wales Medicine Modified proteins
AU692434B2 (en) * 1993-01-21 1998-06-11 President And Fellows Of Harvard College Methods and diagnostic kits utilizing mammalian stress promoters to determine toxicity of a compound
WO1994023039A1 (en) * 1993-04-07 1994-10-13 The Institute Of Cancer Research: Royal Cancer Hospital Methods for screening of substances for therapeutic activity and yeast for use therein
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein

Also Published As

Publication number Publication date
ATE400651T1 (de) 2008-07-15
EP0759170A4 (en) 1997-07-16
EP0759170B1 (en) 2008-07-09
AU7795794A (en) 1995-03-27
JP3810791B2 (ja) 2006-08-16
EP0759170A1 (en) 1997-02-26
DE69435112D1 (de) 2008-08-21
CA2169298A1 (en) 1995-03-16
JPH09505981A (ja) 1997-06-17
AU694745B2 (en) 1998-07-30
WO1995007463A1 (en) 1995-03-16
US6146826A (en) 2000-11-14

Similar Documents

Publication Publication Date Title
KR960705209A (ko) 녹색 형광 단백의 용도
EP2938363B1 (en) Methods and compositions relating to crm197
Sanders et al. Polypeptide translocation across the endoplasmic reticulum membrane.
Li et al. Characterization of two homologous yeast genes that encode mitochondrial iron transporters
Takamatsu et al. Assembly and genetics of spore protective structures
US10975376B2 (en) Protein expression method
JPS59501693A (ja) 組換えｄｎａ分子及びその製造方法
WO2002083906A9 (de) Mhc-tetramere
EP4146687A1 (de) Expression von kollagenpeptid-komponenten in prokaryotischen systemen
Bies et al. A Scj1p homolog and folding catalysts present in dog pancreas microsomes
ES2254043T1 (es) Proteinas fluorescentes y cromoproteinas procedentes de especies hidrozoa que no son aequorea y metodos para usarlas.
RU2005123689A (ru) Флуоресцирующие белки из веслоногих ракообразных и способы их применения
DE10247014A1 (de) MHC-Multimere
EP2816355B1 (de) Detektionsverfahren zur Detektion von Bakterien, Verfahren zur Herstellung von Fusionsproteinen und Fusionsprotein
EA199600001A3 (ru) Агент экстракции периплазматических рекомбинантных белков, способ экстракции периплазматического белка и способ культивирования прокариотического микроорганизма
EP1147419B1 (de) Nachweis von zellen mittels spezifischer zellwand-bindender domänen (cbd) von zellwand-bindenden proteinen
CN110577958B (zh) 核酸、重组质粒、转化株、乙酰胆碱酯酶及其制备方法
Zulkifly et al. Optimisation of recombinant TNFα production in Escherichia coli using GFP fusions and flow cytometry
ATE418604T1 (de) Gegen das prothrombin-fragment f1+2 gerichtete antikörper, ihre herstellung und verwendung
CN109843908A (zh) 脂蛋白输出信号及其用途
CA2226287A1 (en) Novel secretion factors for gram-positive microorganisms, genes encoding them and methods of using it
KR102084054B1 (ko) 재조합 단백질의 분비를 증가시키는 방법
Izadi et al. Producing a mammalian GFP expression vector containing neomycin resistance gene
CN118813436A (zh) 展示单mhc等位基因功能蛋白的工程化细胞
CN116769756A (zh) Pg2-lc蛋白作为snap-25的水解酶的应用

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E902 Notification of reason for refusal
E601 Decision to refuse application