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KR960034425A - Secretory Expression Vectors for Animal Cells - Google Patents

Secretory Expression Vectors for Animal Cells Download PDF

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Publication number
KR960034425A
KR960034425A KR1019950005423A KR19950005423A KR960034425A KR 960034425 A KR960034425 A KR 960034425A KR 1019950005423 A KR1019950005423 A KR 1019950005423A KR 19950005423 A KR19950005423 A KR 19950005423A KR 960034425 A KR960034425 A KR 960034425A
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South Korea
Prior art keywords
animal cells
foreign protein
secretory expression
expression vectors
restriction enzyme
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KR1019950005423A
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Korean (ko)
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KR0143597B1 (en
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김창환
이태호
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성재갑
주식회사 Lg 화학
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명은 인간 사이토메갈로바이러스(cytomegalovirus, CMV)프로모터, 분비를 유도하는 인간 적혈구 형성 자극인자(erythropotetin, EPO)의 시그널 서열 및 외래 단백질을 클로닝할 수 있는 제한효소 인식 서열을 포함하는, 높은 발현율과 분비율을 갖는 동물 세포용 분비형 발현 벡터에 관한 것이다.The present invention provides a high expression rate including a human cytomegalovirus (CMV) promoter, a signal sequence of human erythropotetin (EPO) that induces secretion, and a restriction enzyme recognition sequence capable of cloning foreign proteins. A secretory expression vector for animal cells having a secretion rate.

Description

동물세포용 분비형 발현 벡터Secretory Expression Vectors for Animal Cells

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 발현벡터인 pMI-003의 제작과정과 그 구조를 나타낸 것이고, 제2도는 pMI-003에 의해 형질전환된 동물세포로부터 생산되어 세포외로 분비된 인간 면역 글로불린 G의 Fc 부분을 웨스턴 분석으로 확인한 결과이다.Figure 1 shows the fabrication process and its structure of the expression vector pMI-003, Figure 2 shows the Fc portion of human immunoglobulin G produced from animal cells transformed by pMI-003 and secreted by Western analysis. The result is confirmed.

Claims (3)

인간 사이토메갈로 바이러스(cytomegalovirus) 프로모터, 인간 적혈구 형성 자극인자(erythropoietin)의 시그널 서열 및 외래 단백질 유전자를 클로닝할 수 있는 제한효소 인식 서열을 포함하는 동물세포용 분비형 발현 벡터.A secretory expression vector for animal cells comprising a human cytomegalovirus promoter, a signal sequence of human erythropoietin, and a restriction enzyme recognition sequence capable of cloning foreign protein genes. 제1항에 있어서, 발현벡터 pMI-003(KCTC 0152BP).The expression vector pMI-003 (KCTC 0152BP) according to claim 1. 제1항 또는 제2항의 벡터에 포함된 제한효소 인식서열을 이용하여 상기 시그널 서열 아래에 상기 외래 단백질 유전자를 해독 프레임 맞게 연결한 후 동물세포에서 발현시켜 분비된 상기 외래 단백질을 회수함을 포함하는, 상기 외래 단백질의 제조 방법.Using the restriction enzyme recognition sequence included in the vector of claim 1 or 2 to connect the foreign protein gene under the signal sequence to fit the frame of translation, and then expressing in the animal cell to recover the secreted foreign protein. , Manufacturing method of the foreign protein. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950005423A 1995-03-16 1995-03-16 Secretory Expression Vectors for Animal Cells Expired - Lifetime KR0143597B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950005423A KR0143597B1 (en) 1995-03-16 1995-03-16 Secretory Expression Vectors for Animal Cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950005423A KR0143597B1 (en) 1995-03-16 1995-03-16 Secretory Expression Vectors for Animal Cells

Publications (2)

Publication Number Publication Date
KR960034425A true KR960034425A (en) 1996-10-22
KR0143597B1 KR0143597B1 (en) 1998-07-01

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KR1019950005423A Expired - Lifetime KR0143597B1 (en) 1995-03-16 1995-03-16 Secretory Expression Vectors for Animal Cells

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KR0143597B1 (en) 1998-07-01

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