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KR960029769A - Novel Antimicrobial Protein Isolated from the Larvae of Chinese Cabbage (Pieris rapae) - Google Patents

Novel Antimicrobial Protein Isolated from the Larvae of Chinese Cabbage (Pieris rapae) Download PDF

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Publication number
KR960029769A
KR960029769A KR1019950000234A KR19950000234A KR960029769A KR 960029769 A KR960029769 A KR 960029769A KR 1019950000234 A KR1019950000234 A KR 1019950000234A KR 19950000234 A KR19950000234 A KR 19950000234A KR 960029769 A KR960029769 A KR 960029769A
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KR
South Korea
Prior art keywords
antimicrobial
larvae
antimicrobial protein
present
pieris rapae
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Application number
KR1019950000234A
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Korean (ko)
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KR0139091B1 (en
Inventor
여성문
태건식
방인석
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여성문
태건식
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Priority to KR1019950000234A priority Critical patent/KR0139091B1/en
Publication of KR960029769A publication Critical patent/KR960029769A/en
Application granted granted Critical
Publication of KR0139091B1 publication Critical patent/KR0139091B1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10—Animals; Substances produced thereby or obtained therefrom
    • A01N63/14—Insects

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명은 배추흰나비(Pieris rapae) 유충으로부터 유도하여 분리한 신규한 항균단백질에 관한 것이다. 본 발명의 항균단백질은 박테리아를 용균시킴으로써 그람 음성균과 그람 양성균 모두에 대하여 항균활성을 나타내며 특히, 그람 음성균인 대장균에 대해서 보다 강한 항균작용을 나타내었다. 아울러, 본 발명의 항균단백질은 분자량이 약 4,000달톤이고, 아미노산 분석으로 세크로핀계에 속함을 확인하였다. 본 발명의 항균 단백질은 박테리아에 대하여 항균활성을 나타내므로, 항균제로 이용될 수 있을 것이다.The present invention relates to a novel antimicrobial protein isolated from the cabbage butterfly (Pieris rapae) larvae. The antimicrobial protein of the present invention showed antimicrobial activity against both Gram-negative bacteria and Gram-positive bacteria by lysing bacteria, and particularly, showed stronger antimicrobial activity against Gram-negative bacteria Escherichia coli. In addition, the antimicrobial protein of the present invention has a molecular weight of about 4,000 Daltons, and it was confirmed that it belongs to the secropin system by amino acid analysis. Since the antimicrobial protein of the present invention exhibits antimicrobial activity against bacteria, it may be used as an antimicrobial agent.

Description

배추흰나비(Pieris rapae) 유충으로부터 분리한 신규한 항균단백질Novel Antimicrobial Protein Isolated from the Larvae of Chinese Cabbage (Pieris rapae)

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 배추흰나비 유충의 면역혈림프가 대장균(E. coli)에 대해 항균활성이 있음을 나타내는 그래프이다. 제2도는 배추흰나비 유충의 면역혈림프가 바실러스 서브틸리스(Bacillus subtilis)에 대해 항균활성이 있음을 나타내는그래프이다. 제3도는 배추흰나비 유충의 면역혈림프가 박테리아를 용균시킴을 나타내는 그래프이다. 제4도는 면역혈림프를 트립신으로 처리하여 항균활성을 측정한 결과를 나타내는 사진이다. 제5도는 CM-세파로스 컬럼에서 수득한 용출액을 PepRPC HR 5/5 컬럼을 이용하여 역상 FPLC한 결과를 나타내는 그래프이다.FIG. 1 is a graph showing that the immune hemolymph of cabbage butterfly larvae has antimicrobial activity against E. coli. 2 is a graph showing that the immune hemolymph of Chinese cabbage butterfly larvae has antimicrobial activity against Bacillus subtilis. 3 is a graph showing that the immune hemolymph of cabbage butterfly larva lyses bacteria. Figure 4 is a photograph showing the results of measuring the antimicrobial activity of the treatment of immune hemolymph with trypsin. 5 is a graph showing the results of reverse phase FPLC of the eluate obtained from the CM-Sepharose column using a PepRPC HR 5/5 column.

Claims (1)

다음과 같은 아미노산 서열을 가지는 배추흰나비(Pieris rapae)의 유충으로부터 분리한 항균단백질 Hinnavin I :Antibacterial protein Hinnavin I isolated from the larvae of the cabbage butterfly (Pieris rapae) having the following amino acid sequence: GWKIGKKLEHMGQNIRDGLISAGPAVFAVGQAATIYAAAKGWKIGKKLEHMGQNIRDGLISAGPAVFAVGQAATIYAAAK (상기에서, 아미노산 서열은 IUPAC-IUB의 명명법에 따라 기재하였다.)(In the above, the amino acid sequence is described according to the nomenclature of IUPAC-IUB.) ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950000234A 1995-01-07 1995-01-07 Novel anti-bacterial polypeptide from pieris rapae KR0139091B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950000234A KR0139091B1 (en) 1995-01-07 1995-01-07 Novel anti-bacterial polypeptide from pieris rapae

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950000234A KR0139091B1 (en) 1995-01-07 1995-01-07 Novel anti-bacterial polypeptide from pieris rapae

Publications (2)

Publication Number Publication Date
KR960029769A true KR960029769A (en) 1996-08-17
KR0139091B1 KR0139091B1 (en) 1998-04-30

Family

ID=19406514

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019950000234A KR0139091B1 (en) 1995-01-07 1995-01-07 Novel anti-bacterial polypeptide from pieris rapae

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KR (1) KR0139091B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112375131A (en) * 2020-11-16 2021-02-19 西南大学 Truncation of Seroin protein and application thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112375131A (en) * 2020-11-16 2021-02-19 西南大学 Truncation of Seroin protein and application thereof

Also Published As

Publication number Publication date
KR0139091B1 (en) 1998-04-30

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