KR20240146812A - Cosmetic composition for skin moisturizing and skin soothing containing fermented plant seeds extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin moisturizing and soothing containing a complex fermented extract of plant seeds, and more particularly, to a cosmetic composition exhibiting excellent skin moisturizing and skin soothing effects containing, as an active ingredient, a complex fermented extract of seeds prepared by fermenting and extracting basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds, and quinoa seeds with lactic acid bacteria.

Description

{Cosmetic composition for skin moisturizing and skin soothing containing fermented plant seeds extract}

The present invention relates to a cosmetic composition for skin moisturizing and soothing containing a complex fermented extract of plant seeds, and more particularly, to a cosmetic composition exhibiting excellent skin moisturizing and skin soothing effects containing, as an active ingredient, a complex fermented extract of seeds prepared by fermenting and extracting basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds, and quinoa seeds with lactic acid bacteria.

The outermost layer of the skin, the stratum corneum, protects the skin from physical and chemical stimuli and microorganisms from the outside and maintains homeostasis. If the moisture balance of this skin maintenance is disrupted, the skin begins to dry out. When human skin becomes dry, the cell recovery ability decreases, and it is exposed to ultraviolet rays and active oxygen, and skin aging progresses. As a result, symptoms of skin atrophy and loss of elasticity appear. Recently, as we enter an aging society, research on preventing skin aging or improving aged skin has been steadily increasing.

Normally, human skin is stimulated for a long time by external factors such as cosmetics and air pollution, and internal factors such as stress, aging, and hormones, which increases skin sensitivity. Skin with increased sensitivity develops symptoms such as skin inflammation, itching, and dry skin. In addition, the skin barrier function is weakened by stimulation, and substances that cause irritation penetrate the stratum corneum with weakened barrier function, causing inflammation and dryness, which further worsens the skin condition.

Mucilage is a plant-derived mucus component similar to mucin, a glycoprotein component secreted by the human digestive system and which has the function of supplying moisture to skin tissue. It is an effective component for maintaining skin moisture by preventing moisture loss from the epidermis and strengthening the skin barrier.

Against this backdrop, the inventors of the present invention searched for raw materials that can help moisturize the skin and relieve inflammation, and conducted research on natural moisturizers by utilizing the fact that the rich polysaccharides and mucilage components contained in plant seeds are effective in promoting skin moisturizing. As a result, the inventors confirmed that excellent skin moisturizing and skin soothing activities were exhibited in a seed complex fermentation extract manufactured by fermenting and extracting a complex composed of a specific ratio of basil seed, chia seed, tamarind seed, psyllium seed, flax seed, and quinoa seed, and thus completed the present invention.

(001) Republic of Korea Patent Publication No. 10-2011-0124916 (2011.11.18.) (002) Republic of Korea Patent Publication No. 10-2015-0061124 (201.06.04.) (003) Republic of Korea Patent Publication No. 10-1440844 (2014.09.05.)

The purpose of the present invention is to provide a cosmetic composition containing a plant seed complex fermentation extract as an effective ingredient, which exhibits excellent skin moisturizing and anti-inflammatory effects.

In addition, another purpose of the present invention is to provide a method for producing the plant seed complex fermentation extract.

In order to achieve the above object, according to the present invention, a cosmetic composition containing a complex fermented extract of basil seed, chia seed, tamarind seed, psyllium seed, flax seed and quinoa seed as an effective ingredient is provided.

The above complex fermented extract is characterized in that it is manufactured by fermenting a mixture of basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds, and quinoa seeds with lactic acid bacteria.

The lactic acid bacteria used for fermentation is at least one selected from the group consisting of Lactobacillus plantarum, Lactococcus lactis, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Pediococcus cereviciae, Lactobacillus breve, Leuconostoc mesenteroides, Bifidobacterium bifidum, Lactobacillus sakei, and Lactobacillus crispatus.

The above mixture is characterized in that it is composed of basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds, and quinoa seeds mixed in a weight ratio of 1 to 2:1 to 2:1 to 2:1 to 2:1 to 2:1 to 2:1 to 2, respectively.

The above complex fermentation extract as an effective ingredient is contained in an amount of 0.1 to 20 wt% based on the total weight of the composition.

The above cosmetic composition may contain other active ingredients together with the above complex fermentation extract as an effective ingredient, and preferably may further contain glutathione and sodium chondroitin sulfate.

The above cosmetic composition is characterized as being for moisturizing or soothing the skin.

According to the present invention, in order to achieve the above other objects,

(A) A step of preparing a seed mixture by mixing washed and dried basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds, and quinoa seeds;

(B) a step of placing the seed mixture in a chamber, inoculating it with lactic acid bacteria, and fermenting it at a temperature of 25 to 35°C for 48 to 72 hours; and

(C) A method for producing a complex fermented extract of basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds, and quinoa seeds is provided, which comprises a step of adding a solvent to a fermented seed mixture and extracting the mixture at 50 to 60°C for 3 to 5 hours.

The solvent is at least one selected from the group consisting of water, glycerin, butylene glycol, pentylene glycol, dipropylene glycol, propylene glycol, propanediol, isopropylene glycol, methylpropanediol, 1,2-hexanediol, ethylhexanediol, and polyethylene glycol.

The plant seed complex fermented extract of basil seed, chia seed, tamarind seed, psyllium seed, flax seed and quinoa seed of the present invention is safe as a natural extract, and exhibits excellent skin moisturizing effect and anti-inflammatory effect due to its synergistic effect, so it can be usefully used as a natural skin moisturizing and soothing cosmetic for people with sensitive and dry skin.

Figure 1 is a graph showing the mucilage content of an extract manufactured in an example of the present invention.
Figure 2 is a graph showing the moisturizing activity of an extract manufactured in an example of the present invention.
Figure 3 is a graph showing the anti-inflammatory factor activity of the extract manufactured in the example of the present invention.

The present invention is described in detail below.

The present invention is characterized in that a complex fermented extract of plant seeds, comprising basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds, and quinoa seeds, exhibits excellent skin moisturizing and soothing effects due to the synergistic effect.

Basil seed (Ocimum basilicum seed) is a seed of the natural plant herb basil, which is a highly hydrophilic seed that absorbs about 30 times its weight in water, and is effective in removing toxins from the body. It has been used traditionally for therapeutic purposes to improve blood, and is rich in antioxidants and dietary fiber, so it has the effect of preventing obesity and relieving skin inflammation.

Chia seeds (Salvia hispanica seeds) have been a staple food in American cultures since ancient times, and have excellent hygroscopic properties, absorbing about 12 times their weight in water. They are effective in preventing constipation due to their rich dietary fiber content, improve blood circulation, promote skin metabolism, and have powerful antioxidant properties that remove harmful oxygen radicals from the body and help skin regeneration.

Tamarind seeds (Tamarindus Indica Seeds) are also called Indian dates and are rich in nutrients such as potassium, iron, magnesium, and protein. Procyanidin, flavonoid, and polyphenol contained in tamarind seeds act as antioxidants and protect skin cells by removing active oxygen in the body, thereby slowing down the aging process.

Psyllium asiatica (Plantago asiatica Seed) is a wild plant with strong vitality, also known as majeoncho, and is the dried seed of the medicinal plant psyllium, which has diuretic, antipyretic, expectorant, and sedative effects. Psyllium asiatica has the effect of suppressing skin fungus, and helps relieve inflammation such as skin ulcers.

Flaxseed (Linumusitatissimum Seed) is the seed of a fiber crop called flax, and is rich in healthy fatty acids, antioxidants, and fiber, and has excellent antioxidant and antibacterial effects. In particular, it is known to contain hundreds of times more lignans, which are rich in phytoestrogens and antioxidants, than other plants.

Quinoa seed (Chenopodium Quinoa Seed) has been cultivated since the ancient Incan civilization and is called the mother of grains. It is rich in selenium, a powerful antioxidant, and helps prevent skin aging by removing active oxygen in the body.

The compound fermented extract of basil seed, chia seed, tamarind seed, psyllium seed, flax seed and quinoa seed, which is an effective ingredient of the present invention, prepared by mixing the seeds in a specific ratio and then fermenting them with lactic acid bacteria, was confirmed to exhibit superior skin moisturizing and soothing activities due to the synergistic effect of mixing and the activity enhancement effect through fermentation.

According to one specific example of the present invention, the composite fermented extract of basil seed, chia seed, tamarind seed, psyllium seed, flax seed and quinoa seed is prepared by the following method.

(A) A step of preparing a seed mixture by mixing washed and dried basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds, and quinoa seeds;

(B) a step of placing the seed mixture in a chamber, inoculating it with lactic acid bacteria, and fermenting it at a temperature of 25 to 35°C for 48 to 72 hours; and

(C) It is manufactured by a method including a step of adding a solvent to a fermented seed mixture and extracting it at 50 to 60°C for 3 to 5 hours.

Preferably, the mixture of basil seeds, chia seeds, tamarind seeds, chamomile seeds, flax seeds and quinoa seeds is mixed in a weight ratio of 1 to 2:1 to 2:1 to 2:1 to 2:1 to 2:1 to 2, respectively, and is more preferable when it is mixed in a weight ratio of 2:2:1:1:1:1, respectively, because it exhibits a more excellent moisturizing and soothing effect.

As the lactic acid bacteria used for fermentation of the seed mixture, at least one selected from the group consisting of Lactobacillus plantarum, Lactococcus lactis, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Pediococcus cereviciae, Lactobacillus breve, Leuconostoc mesenteroides, Bifidobacterium bifidum, Lactobacillus sakei, and Lactobacillus crispatus is used, more preferably Lactobacillus plantarum. plantarum) is used.

In the step (C) above, at least one selected from the group consisting of water, glycerin, butylene glycol, pentylene glycol, dipropylene glycol, propylene glycol, propanediol, isopropylene glycol, methylpropanediol, 1,2-hexanediol, ethylhexanediol, and polyethylene glycol is used as an extraction solvent.

According to another specific example of the present invention, the composite fermented extract of basil seed, chia seed, tamarind seed, psyllium seed, flax seed and quinoa seed as the effective ingredients may be prepared by preparing each fermented extract and then mixing them at the above ratio.

The seed complex fermentation extract manufactured in this manner exhibited a high mucilage content (Test Example 1), excellent moisturizing effects (Test Examples 2, 3, 5), and anti-inflammatory effects (Test Example 4). Therefore, a cosmetic composition containing this as an effective ingredient can be used for moisturizing or soothing the skin.

The seed complex fermentation extract as an effective ingredient is contained in an amount of 0.1 to 20 wt% based on the total weight of the composition.

The above cosmetic composition may contain other active ingredients together with the above complex fermentation extract as an effective ingredient, and preferably may further contain glutathione and sodium chondroitin sulfate.

The cosmetic composition of the present invention may contain, in addition to the seed complex fermentation extract, an excipient commonly used in the field of cosmetics or dermatology, such as a fatty substance, a surfactant, a preservative, a vitamin, a humectant, water, an emulsifier, an essential oil, or any other ingredient commonly used in cosmetics. And the above ingredients may be introduced in an amount commonly used in the field of dermatology.

The formulation of the cosmetic composition of the present invention is not limited, but can be prepared in the form of a general emulsified formulation and a solubilized formulation. Cosmetics in the form of emulsified formulation include lotions, creams, essences, serums, etc., and cosmetics in the form of solubilized formulation include tonics, packs, skins, low-viscosity gels, etc.

[Example]

Hereinafter, the present invention will be described in more detail based on the following examples and test examples. However, the following examples are only intended to illustrate the present invention, and the present invention is not limited by the following examples.

Comparative Examples 1-3: Preparation of seed mixture extract

First, washed and dried basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds, and quinoa seeds were mixed in the ratio shown in Table 1 below to prepare a seed mixture. The mixture was placed in a chamber, purified water corresponding to 10 times the dry weight was added, and extraction was performed at 50°C for 4 hours. After filtering, the mixture was concentrated using a reduced pressure evaporator and freeze-dried using a freeze dryer to prepare an extract powder.

Examples 1-2: Preparation of seed complex fermentation extract

Washed and dried basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds, and quinoa seeds were mixed in the ratio shown in Table 1 below to prepare a seed mixture.

The above mixture was placed in a chamber, purified water corresponding to 10 times the dry weight was added, Lactobacillus plantarum was inoculated, and fermented at a temperature of 25℃ for 72 hours. The fermented seed fermentation mixture was extracted with purified water in a chamber at 50℃ for 4 hours. This was concentrated using a reduced pressure evaporator and then freeze-dried using a freeze dryer to prepare a seed complex fermentation extract.

(Weight ratio) Basil seeds Chia seeds Tamarind seeds Electric car Flaxseed Quinoa seeds Comparative Example 1 2 2 1 1 1 1 Comparative Example 2 1 2 2 1 1 1 Comparative Example 3 1 1 1 2 2 2 Example 1 2 2 1 1 1 1 Example 2 1 2 2 1 1 1

Test Example 1: Comparison of Mucilage Content

The mucilage content of the extracts manufactured in Comparative Examples 1 to 3 and Examples 1 to 2 was measured, and the results are shown in Fig. 1. The mucilage content in Comparative Example 1 was confirmed to be relatively higher than in Comparative Examples 2 and 3, but the mucilage content in Example 1, which was extracted by fermentation with lactic acid bacteria, was confirmed to be the highest.

Test Example 2: Measurement of the increase rate of expression of HAS-1 and HAS-3

The moisturizing activity of the extracts prepared in Comparative Examples 1 to 3 and Examples 1 to 2 was measured using the increase rate of hyaluronic acid synthesis enzyme from keratinocytes.

Keratinocytes (HaCaT) were seeded in a 12-well plate at 5 × 10 ⁵ cells/well using DMEM medium supplemented with 10% FBS and cultured for 18 hours. After culture, the samples were treated and cultured for 24 hours. RNA was extracted from the cultured cells, and the expression increase rate of Hyaluronan Synthase 1 (HAS-1) and Hyaluronan Synthase 3 (HAS-3) was confirmed through Reverse transcription polymerase chain reaction (RT-PCR). The expression increase rate was calculated by the following formula, and the results are shown in Fig. 2. For the negative control group, medium was used instead of the sample, and for the positive control group, hyaluronic acid was used.

Hyaluronic acid synthase increase rate (%) = (Sample HAS expression amount/negative control HAS expression amount) × 100

Compared to the comparative examples above, the examples showed a higher increase rate of hyaluronic acid synthase, and among them, the extract of Example 1, in which basil seed, chia seed, tamarind seed, psyllium seed, flax seed, and quinoa seed were mixed in a weight ratio of 2:2:1:1:1:1, respectively, and then fermented and extracted with Lactobacillus plantarum, showed the highest increase rate.

Test Example 3: Measurement of hyaluronic acid production

The extracts manufactured in Comparative Examples 1 to 3 and Examples 1 to 2 were used as samples to measure the amount of hyaluronic acid produced. The hyaluronic acid produced using HaCaT cells was measured. HaCaT cells were seeded at a concentration of 1×10 ⁶ cells/well in a 6-well plate and cultured, then replaced with a medium not supplemented with FBS, starved for 18 hours, and cultured for 24 hours. The cells were treated with each sample at the same concentration, and after culturing for 24 hours, the culture fluid was harvested and the amount of hyaluronic acid produced was measured using an ELISA kit. The HA production rate (%) is shown in Table 2 below.

division Treatment concentration (%) HA production rate (%) Comparative Example 1 1.0 35.7 Comparative Example 2 1.0 32.3 Comparative Example 3 1.0 31.8 Example 1 1.0 46.6 Example 2 1.0 40.8 Hyaluronic acid 1.0 52.8

As confirmed in Table 2 above, when the comparative example and the example sample were treated at the same concentration, the seed complex fermentation extract of the example of the present invention showed a higher hyaluronic acid production rate.

Test Example 4: Measurement of Inflammation-Related Factor Inhibition Activity

The anti-inflammatory factor inhibitory activity of the extracts prepared in Comparative Examples 1 to 3 and Examples 1 to 2 was measured using the rate of decrease in the expression of inflammatory mediators from keratinocytes.

Keratinocytes (HaCaT) were adjusted to 5× ¹⁰⁵ cells/well using DMEM medium supplemented with 10% FBS, seeded in 12-well plates, and cultured for 18 hours. Then, they were treated with 10 ng/mL LPS and samples and cultured for 24 hours. The cultured cells were examined for mRNA expression levels using RT-PCR. The mRNA expression levels increased by LPS stimulation were used as a control group, and the expression reduction rate was calculated by the following formula, and the results are shown in Figure 3 below.

Expression reduction rate (%) = [1-(sample mRNA expression amount/control mRNA expression amount)] × 100

Compared to the comparative examples above, the sample of the example showed a higher COX-2 expression reduction rate, and the composite fermentation extract of Example 1 showed a more excellent effect.

Examples 3-4: Preparation of serum containing seed complex fermentation extract

A serum containing the seed complex fermentation extract prepared in Example 1 was prepared by a conventional method according to the composition shown in Table 3 below.

Ingredients Content (weight%) Contrast example 1 Example 3 Example 4 Seed complex fermentation extract
(Example 1) - 5.0 5.0 Seed Mix Extract
(Comparative Example 1) 5.0 - - Glutathione - - 0.2 Sodium chondroitin sulfate - - 0.3 Carbomer 0.1 0.1 0.1 Methyl Gluceth-20 2.0 2.0 2.0 Glyceres-26 2.5 2.5 2.5 Jojoba oil 2.0 2.0 2.0 MCT Oil 1.0 1.0 1.0 Dimethicone 2.0 2.0 2.0 Citric acid 0.3 0.3 0.3 Propylene glycol 5.0 5.0 5.0 Butylene glycol 3.0 3.0 3.0 1,2-Hexanediol 2.0 2.0 2.0 Allantoin 0.2 0.2 0.2 Betaine 0.2 0.2 0.2 incense a very small amount a very small amount a very small amount Purified water Remaining amount Remaining amount Remaining amount

Test Example 5: Skin Moisture Improvement Evaluation

In order to confirm the effect of improving skin moisturizing, 10 subjects were divided into groups and the serums of Control Example 1, Example 3, and Example 4 were applied twice a day (morning and evening) for 4 weeks. After 4 weeks, the degree of improvement in moisture content was evaluated using Corneometer CM825, and the results are shown in Table 4.

division Initial Average(A.U) Average (A.U) after 4 weeks Increase in moisture content (%) Contrast example 1 34.77 36.72 3.4 Example 3 32.05 41.74 17.9 Example 4 33.66 43.82 18.7

As confirmed in Table 4 above, the serum of Example 3 including the complex fermented extract of basil seed, chia seed, tamarind seed, psyllium seed, flax seed and quinoa seed (Example 1) of the present invention showed a much higher skin moisturizing improvement effect compared to the serum of Control Example 1 containing the seed mixed extract (Comparative Example 1) mixed and extracted at the same weight ratio. In addition, the serum of Example 4 containing glutathione and sodium chondroitin sulfate together with the seed complex fermented extract of Example 1 showed a higher skin moisturizing improvement effect compared to the serum of Example 3 which did not contain glutathione and sodium chondroitin sulfate.

Claims (10)

A cosmetic composition containing a complex fermented extract of basil seed, chia seed, tamarind seed, psyllium seed, flax seed and quinoa seed as an effective ingredient. A cosmetic composition characterized in that in claim 1, the complex fermented extract is manufactured by extracting a mixture of basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds, and quinoa seeds by fermenting them with lactic acid bacteria. A cosmetic composition in claim 2, characterized in that the lactic acid bacteria is at least one selected from the group consisting of Lactobacillus plantarum, Lactococcus lactis, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Pediococcus cereviciae, Lactobacillus breve, Leuconostoc mesenteroides, Bifidobacterium bifidum, Lactobacillus sakei, and Lactobacillus crispatus. A cosmetic composition characterized in that in the second paragraph, the mixture is formed by mixing basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds, and quinoa seeds in a weight ratio of 1 to 2:1 to 2:1 to 2:1 to 2:1 to 2:1 to 2:1 to 2, respectively. A cosmetic composition, characterized in that in claim 1, the complex fermentation extract is contained in an amount of 0.1 to 20 wt% based on the total weight of the composition. A cosmetic composition according to claim 5, characterized in that the cosmetic composition further contains glutathione and sodium chondroitin sulfate. A cosmetic composition according to claim 1, characterized in that the cosmetic composition is for moisturizing the skin. A cosmetic composition according to claim 1, characterized in that the cosmetic composition is for skin soothing. (A) A step of preparing a seed mixture by mixing washed and dried basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds, and quinoa seeds;
(B) a step of placing the seed mixture in a chamber, inoculating it with lactic acid bacteria, and fermenting it at a temperature of 25 to 35°C for 48 to 72 hours; and
(C) A method for producing a complex fermented extract of basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds and quinoa seeds, comprising the step of adding a solvent to a fermented seed mixture and extracting at 50 to 60°C for 3 to 5 hours. A method for producing a composite fermented extract of basil seeds, chia seeds, tamarind seeds, psyllium seeds, flax seeds and quinoa seeds, characterized in that in claim 9, the solvent is at least one selected from the group consisting of water, glycerin, butylene glycol, pentylene glycol, dipropylene glycol, propylene glycol, propanediol, isopropylene glycol, methylpropanediol, 1,2-hexanediol, ethylhexanediol and polyethylene glycol.