KR20240007322A - Ruvc 도메인이 존재하는 효소 - Google Patents

Ruvc 도메인이 존재하는 효소 Download PDF

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Publication number: KR20240007322A
Authority: KR; South Korea
Prior art keywords: sequence; seq; endonuclease; cases; nos
Prior art date: 2019-02-14

Application number

KR1020247000311A

Other languages

English (en)

Korean (ko)

Inventor

브라이언 토마스

크리스토퍼 브라운

로즈 칸터

아우드라 드보토

크리스티나 버터필드

리사 알렉산더

대니엘라 에스 에이 골츠만

제이슨 리우

Original Assignee

메타지노미, 인크.

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2019-02-14

Filing date

2020-02-14

Publication date

2024-01-16

2020-02-14 Application filed by 메타지노미, 인크. filed Critical 메타지노미, 인크.

2024-01-16 Publication of KR20240007322A publication Critical patent/KR20240007322A/ko

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KR1020247000311A 2019-02-14 2020-02-14 Ruvc 도메인이 존재하는 효소 KR20240007322A (ko)

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US201962805899P	2019-02-14	2019-02-14
US201962805878P	2019-02-14	2019-02-14
US201962805868P	2019-02-14	2019-02-14
US62/805,868		2019-02-14
US62/805,878		2019-02-14
US62/805,899		2019-02-14
US201962874414P	2019-07-15	2019-07-15
US62/874,414		2019-07-15
KR1020217028874A KR102623312B1 (ko)	2019-02-14	2020-02-14	Ruvc 도메인이 존재하는 효소
PCT/US2020/018432 WO2020168291A1 (en)	2019-02-14	2020-02-14	Enzymes with ruvc domains

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CN (2)	CN116515797A (zh)
AU (2)	AU2020223370B2 (zh)
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Publication number	Priority date	Publication date	Assignee	Title
CN116515797A (zh) *	2019-02-14	2023-08-01	宏基因组学公司	具有ruvc结构域的酶
US10982200B2 (en)	2019-02-14	2021-04-20	Metagenomi Ip Technologies, Llc	Enzymes with RuvC domains
GB2594339B (en) *	2020-02-14	2023-02-08	Metagenomi Inc	Enzymes with RUVC domains
MX2022011039A (es)	2020-03-06	2022-12-13	Metagenomi Inc	Sistemas crispr clase ii tipo v.
CA3177051A1 (en) *	2020-03-31	2021-10-07	Metagenomi, Inc.	Class ii, type ii crispr systems
WO2021226369A1 (en) *	2020-05-08	2021-11-11	Metagenomi Ip Technologies, Llc	Enzymes with ruvc domains
WO2022056301A1 (en) *	2020-09-11	2022-03-17	Metagenomi Ip Technologies, Llc	Base editing enzymes
WO2022098681A2 (en) *	2020-11-03	2022-05-12	Caspr Biotech Corporation	Novel class 2 crispr-cas rna-guided endonucleases
EP4281554A1 (en) *	2021-01-22	2023-11-29	Metagenomi, Inc.	Novel engineered and chimeric nucleases
WO2022159742A1 (en) *	2021-01-22	2022-07-28	Metagenomi, Inc	Novel engineered and chimeric nucleases
KR20240004618A (ko) *	2021-04-30	2024-01-11	메타지노미, 인크.	Ruvc 도메인을 갖는 효소
WO2023122663A2 (en) *	2021-12-23	2023-06-29	Mammoth Biosciences, Inc.	Effector proteins and methods of use
WO2023194359A1 (en) *	2022-04-04	2023-10-12	Alia Therapeutics Srl	Compositions and methods for treatment of usher syndrome type 2a
WO2023245141A2 (en) *	2022-06-15	2023-12-21	Beam Therapeutics Inc.	Compositions and methods for reducing complement activation
WO2024056880A2 (en) *	2022-09-16	2024-03-21	Alia Therapeutics Srl	Enqp type ii cas proteins and applications thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
KR20150105634A (ko) *	2012-12-12	2015-09-17	더 브로드 인스티튜트, 인코퍼레이티드	서열 조작을 위한 개선된 시스템, 방법 및 효소 조성물의 유전자 조작 및 최적화
WO2016196655A1 (en) *	2015-06-03	2016-12-08	The Regents Of The University Of California	Cas9 variants and methods of use thereof
WO2016205759A1 (en) *	2015-06-18	2016-12-22	The Broad Institute Inc.	Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
US20210166783A1 (en) *	2016-08-17	2021-06-03	The Broad Institute, Inc.	Methods for identifying class 2 crispr-cas systems
KR20200124702A (ko) *	2018-02-23	2020-11-03	파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드	신규한 cas9 오르소로그
CN116515797A (zh)	2019-02-14	2023-08-01	宏基因组学公司	具有ruvc结构域的酶

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MX2023006575A (es)	2023-06-16
US20240117330A1 (en)	2024-04-11
AU2023206079A1 (en)	2023-08-10
CN113728098A (zh)	2021-11-30
JP7502537B2 (ja)	2024-06-18
JP2022520428A (ja)	2022-03-30
CA3241703A1 (en)	2020-08-20
KR102623312B1 (ko)	2024-01-09
JP2023179468A (ja)	2023-12-19
CA3130135A1 (en)	2020-08-20
WO2020168291A1 (en)	2020-08-20
KR20210139254A (ko)	2021-11-22
EP3924482A4 (en)	2023-04-05
JP2024133476A (ja)	2024-10-02
EP3924482A1 (en)	2021-12-22
CN116515797A (zh)	2023-08-01
AU2020223370B2 (en)	2023-04-20
MX2021009886A (es)	2021-10-13
AU2020223370A1 (en)	2021-10-07

Publication	Publication Date	Title
KR102623312B1 (ko)	2024-01-09	Ruvc 도메인이 존재하는 효소
US12024727B2 (en)	2024-07-02	Enzymes with RuvC domains
US20240209332A1 (en)	2024-06-27	Enzymes with ruvc domains
US20220298494A1 (en)	2022-09-22	Enzymes with ruvc domains
WO2021178934A1 (en)	2021-09-10	Class ii, type v crispr systems
US20220220460A1 (en)	2022-07-14	Enzymes with ruvc domains
US20240352433A1 (en)	2024-10-24	Enzymes with hepn domains
WO2021226369A1 (en)	2021-11-11	Enzymes with ruvc domains
WO2023039377A1 (en)	2023-03-16	Class ii, type v crispr systems
US20240110167A1 (en)	2024-04-04	Enzymes with ruvc domains
GB2617659A (en)	2023-10-18	Enzymes with RUVC domains
KR20240110597A (ko)	2024-07-15	엔도뉴클레아제 시스템

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