KR20230034674A - Method for preparing leaf tea of Gynostemma pentaphyllum using fresh leaf and method for preparing Gynostemma pentaphyllum extracts using the leaf tea prepared thereby - Google Patents

Abstract

The present invention relates to a method for preparing a Gynostemma pentaphyllum leaf tea which has a high content of a low glycoside saponin and has benzopyrene reduced, and a method for preparing a Gynostemma pentaphyllum leaf tea extract using the Gynostemma pentaphyllum leaf tea prepared thereby. The Gynostemma pentaphyllum leaf tea prepared by the present invention contains a low glycoside saponin which is converted from high glycoside saponins such that Gynostemma pentaphyllum leaves can be used in various methods. In addition, the present invention has a low molecular active saponin with a high content even infusing in a general method without treatment of high pressure/high temperature, thereby being very useful. Moreover, the method for a Gynostemma pentaphyllum leaf tea extract, of the present invention, has an improved extract yield with high content of low-molecular active saponins, such as ginsenoside Rg3, gypenoside L, gypenoside LI, and the like, without treatment of high pressure and/or a high temperature, and can usefully obtain a Gynostemma pentaphyllum leaf tea extract with low benzopyrene content.

Description

Method for preparing leaf tea of Gynostemma pentaphyllum using fresh leaf and method for preparing Gynostemma pentaphyllum extracts using the leaf tea prepared thereby}

The present invention relates to a method for preparing periwinkle leaf tea containing a high content of low glycoside saponin and reduced benzopyrene, and a method for producing an edema leaf tea extract using the edema leaf tea prepared according to the method.

Doloe leaves contain a large amount of saponin glycosides, so they have been widely used as a substitute for ginseng, drinking in the form of tea, or as a raw material for nourishing tonics.

It is well known through previous studies that the saponins, jiphenoside and ginsenosides, contained in ginseng leaf, show the effect of reducing body fat through the activation of AMPK, lowering blood sugar, improving or treating hyperlipidemia, and improving exercise performance. .

By the way, the saponins contained in the leaves are mainly glycosides, and the high glycosides saponins have a high molecular weight and strong polarity, making it difficult to penetrate biological membranes, resulting in low bioavailability. Specifically, when raw leaves are used as they are, bioavailability is very low because the precursors of low-molecular-weight effective saponins present in various ways in the raw leaves are not sufficiently activated. There have been reports of saponins that have not been converted into hypoglycosides converted into hypoglycosidic saponins by intestinal microorganisms. It is emphasized that the process of converting glycoside saponin is necessary.

On the other hand, doloe leaves have been mainly used in the form of doloeip tea prepared by drying after roasting. The roasting process in the process of manufacturing ginguli leaf tea is to separate active ingredients bound to plant tissues from tissues through a high-temperature heat treatment process, and to prevent enzymes present in plant leaves from being activated and decomposing active ingredients after harvesting. . In this regard, Prior Document 1 (Japanese Patent Laid-open Publication No. 1983-170447; processing method of periwinkle) also discloses a method for processing dandelion leaves.

However, since the conventional roasting treatment method is for enzyme inactivation, sterilization of microorganisms, and rapid drying, etc., it is treated for a relatively short time, so there is insufficient time for high glycoside saponin to be sufficiently converted into low molecular weight saponin. In addition, since the main purpose of the roasting process itself is drying, there is a problem in that sufficient desugarization and hydrolysis do not occur due to loss of moisture during the roasting process. Accordingly, there is a limit to the fact that the conventional sedum leaf tea cannot be consumed in a form containing a high content of low-molecular effective saponin.

Meanwhile, in the case of the roasting process, it is well known that benzopyrene may be generated due to high temperature treatment. Since benzopyrene is known as a carcinogen, and 10 ppb or less of benzopyrene is set as an acceptable standard in Europe, a plan to reduce benzopyrene is required in manufacturing tea.

Currently, various methods have been disclosed for increasing the low-molecular effective saponin content of the extract of the ginguli leaf extract, but there is a lack of research on the method for increasing the effective low-molecular-weight saponin content of the leaf tea, which is in greater demand.

Therefore, in the process of manufacturing ginseng leaf tea, the content of dammarin-type low-molecular-weight effective saponins such as ginsenoside Rg3, zipenoside L, zipenoside LI, damulin A, and B, which are major active physiologically active ingredients, is increased while reducing the amount of benzopyrene. A new method of reducing the content is needed.

JP 1983-170447 A KR 10-1986-0000020 A

An object of the present invention is to provide a method for producing ginseng leaf tea in which the high glycoside saponin of ginseng leaf is converted to a low glycoside, and the benzopyrene content is reduced.

Another object of the present invention is to increase the content of effective low-molecular-weight saponins such as ginsenoside Rg3, diphenoside L and diphenoside LI, damulin A and damulin B without high pressure and/or high temperature treatment by using the above-mentioned peony leaf tea. It is an object of the present invention to provide a method for preparing an extract of ginseng leaf tea, in which the extraction yield of low-molecular-weight effective saponin is improved and the content of benzopyrene is low.

In order to achieve the above object, the present invention provides (1) the step of sealing after supplying gynae leaves to a reaction container; (2) heating the inside of the reaction vessel at 112 to 150° C. for 10 minutes to 48 hours; and (3) releasing the pressure in the reaction vessel to dry the periwinkle leaves.

According to one embodiment of the present invention, the outer leaves of the step (1) may be fresh leaves having a moisture content of 80% by weight or more.

According to one embodiment of the present invention, the moisture content of the fresh pediatric leaf in step (1) may be adjusted to 30 to 90% by weight by putting the fresh pediatric leaf in an open container and heating it. In an embodiment of the present invention, According to this, the heating may be performed at 60 to 300 °C.

According to one embodiment of the present invention, (4) drying the leaves so that the moisture content is 2 to 15% by weight; may be additionally included.

According to an embodiment of the present invention, the ginseng leaf tea contains 0.1 to 3.0 mg/g of ginsenoside Rg3, 0.5 to 12 mg/g of zipenoside L, and 0.5 to 8 mg/g of zipenoside LI, based on the weight of solid content. mg/g, 0.5 to 10 mg/g of damulin A and 0.5 to 10 mg/g of damulin B, and 10 ppb or less of benzopyrene.

In addition, the present invention is prepared according to the above method, 0.1 to 3.0 mg/g of ginsenoside Rg3, 0.5 to 12 mg/g of zipenoside L, and 0.5 to 8 mg of zipenoside LI based on the solid weight. / g, 0.5 to 10 mg/g of damulin A and 0.5 to 10 mg/g of damulin B, and 10 ppb or less of benzopyrene.

In addition, the present invention includes (1) supplying gynae leaves to a reaction container and then sealing it; (2) heating the inside of the reaction vessel at 112 to 150° C. for 10 minutes to 48 hours; and (3) performing atmospheric hot water extraction or ethanol extraction of the heated ginseng leaf tea at 40 to 100 ° C.;

According to an embodiment of the present invention, between the steps (2) and (3), (2-1) drying the ginkgo leaves by releasing the pressure in the reaction container may be additionally included.

According to one embodiment of the present invention, between the step (2-1) and the step (3), (2-2) drying the perilla leaf to a moisture content of 2 to 15% by weight; further comprising can do.

According to one embodiment of the present invention, the ginseng leaf tea extract contains 0.5 to 7.0 mg/g of ginsenoside Rg3, 1.5 to 90 mg/g of zipenoside L, 1.5 to 80 mg/g of zipenoside LI, 2.0 to 60 mg/g of damulin A and 2.0 to 60 mg/g of damulin B, and may contain benzopyrene at 10 ppb or less.

In addition, the present invention is prepared according to the above method, 0.5 to 7.0 mg/g of ginsenoside Rg3, 1.5 to 90 mg/g of zipenoside L, 1.5 to 80 mg/g of zipenoside LI, damulin Provided is a peony leaf tea extract comprising 2.0 to 60 mg/g of A and 2.0 to 60 mg/g of damulin B, and 10 ppb or less of benzopyrene.

The leaf tea prepared according to the present invention contains the high glycoside saponin in a state in which it has already been converted into a low glycoside, so that the leaf tea itself can be used in various ways, and can be used in a general way without high pressure and/or high temperature treatment. Even after extraction, a high content of low-molecular-weight effective saponin is extracted, and the content of benzopyrene is low, so it is very useful.

In addition, in the method for preparing an extract of genus japonica leaf tea of the present invention, the extraction yield is also improved while the content of low-molecular effective saponins such as ginsenoside Rg3, zipenoside L, and zipenoside LI is high without high pressure and/or high temperature treatment. It is useful because it is possible to obtain an extract of ginseng leaf tea with a low content of benzopyrene.

1 is a process chart showing a method for manufacturing a peony leaf tea extract according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

Gynostemma pentaphyllum is a perennial vine belonging to the Cucurbitaceae family. It grows naturally in the forests of mountains or fields, and the rhizome extends to the side, has white hairs at the nodes, and grows tangled, but climbs up with tendrils. As a habitat, it grows naturally in the mountains of the southern region, Jeju Island, and Ulleung Island in Korea, and is widely distributed in regions such as China, Japan, and Southeast Asia. Most of them grow in places with high humidity, such as coastal and riversides.

In addition to stones, there are several species depending on the country and region where they grow naturally. Among them, pentaphyllum species are widely distributed, and studies on species, components, and efficacy have been widely conducted. In addition to stones, various saponins are contained, and they are named gynosaponin or gypenoside. When looking at the chemical structure of the diphenoside, only the difference is in which part the OH group is bonded, and the structure has a similar structure to the ginsenoside system of ginseng. Due to the high content of saponin in doloe, doloe leaves have been widely drunk as a nutrient tonic substitute for ginseng since ancient times.

Saponin contained in the outer leaf is known to exhibit effects such as lipid metabolism improving action, cardiovascular disease defense action, blood sugar lowering action, central nervous system action, anticancer action, platelet aggregation inhibitory action, and tonic action. In addition, in addition to gypenoside, which is a saponin, doloes contain primeveoside, sophoroside, bisdesmoside, gentiobioside, rutinoside, etc. It contains glycosides, steroids, sugars and pigments.

Most of the saponin compounds contained in the leaves are in the form of glycosides, including sugars, and are high glycoside saponins with low absorption in vivo. Even saponins that are difficult to absorb can sometimes be converted into effective saponins that can be absorbed with low molecular weight. For example, in the case of red ginseng, which contains saponin similar to that of ginseng leaves, there is a report that the bioavailability is increased by converting the high molecular saponin component into low molecular weight (Korean Patent No. 10-1200571). Therefore, conversion of gypenoside glycosides of ginseng leaves into absorbable and effective gifenosides (non-glycosides or low-molecular-weight saponins with small sugar modifications) is a very important factor in increasing bioavailability and thereby increasing effectiveness.

In the present invention, the effective low molecular weight saponin is also referred to as 'low glycoside saponin' and 'low molecular weight saponin'.

On the other hand, doloe has traditionally been manufactured, stored, distributed, and consumed in the form of dried doloe leaf tea. The purpose of making leaf tea by roasting the leaves is to inactivate the decomposing enzyme remaining in the leaves, to preserve the active ingredients and flavor such as saponin, to sterilize harmful microorganisms remaining in the fresh leaves, and to improve storage stability. It is performed for the purpose of removing water for the purpose of removing moisture and for the purpose of manufacturing it into a tea-shaped lump. In recent years, extracts obtained by extracting this leaf tea and concentrating the saponin content are manufactured, stored, distributed, and consumed as food and health foods.

To explain the general method of manufacturing doloeipcha in more detail, first, doloe leaves are collected, washed in clear water to remove various foreign substances, and dried by hanging on a tray. The moisture content of fresh leaves outside the stone may be 80 to 95%, preferably 85 to 95%, but may vary depending on the place of production and the time of collection. In addition, the dried leaves are naturally dried for about 1 to 2 days, which means that if the moisture content of the leaves is too high, too much steam is generated during the roasting process, leachate is generated, or the roasting time is too long. This is to prevent lengthening. However, during the natural drying process, the decomposition of effective saponin by the decomposing enzyme remaining in the fresh leaves outside the stone may proceed to a certain extent.

On the other hand, the roasting process is traditionally done by putting gypsy leaves in a cauldron heated with wood fire and roasting them while stirring by hand, then taking them out before the outer surface of the gypsy leaves is burned, cooling them for a certain period of time and keeping in mind (making shapes by rubbing them by hand), and then the remaining moisture is evened out again. When it spreads, it proceeds in such a way that it is repeated several times. As a recent automated method, there is also a method of rotating and roasting doloe leaves in a tumbler method using a heated electric heater as a heat source, but this does not form a tea-shaped round shape, so it is It is known to be applied for use as a raw material for leaf tea extract.

Either way, the roasting process takes a short time at high heat and loses moisture, so the conversion to low molecular weight saponin is insignificant due to insufficient moisture required for deglycosylation hydrolysis. In addition, by applying high heat in a low moisture state, excessive formation of benzopyrene cannot be avoided.

As another method for producing doloeip tea, a process of steaming doloeil fresh leaves like red ginseng may be considered. However, the traditional steaming process is aimed at enzyme inactivation, sterilization, and drying of gingulifolia through high-temperature heat treatment, as in the above-mentioned roasting process, and is not aimed at converting high glycoside saponin into low molecular saponin, so low molecular effective saponin It is difficult to expect an increase in content.

Specifically, the process of steaming fresh leaves outside the stone may select a method of boiling and steaming water or a method of providing high-temperature steam of 121 ° C. or higher using a steam generator. However, in steaming by steaming, leachate may occur because the moisture content of the exfoliated leaves is already high, but moisture is supplied again. When leachate is generated, it means that the active ingredients of the leaves are eluted and the loss of the active ingredients may occur. In addition, steaming using a high-temperature steam generator reduces space efficiency due to the large volume of fresh leaves outside the stone and low pile density, and this also cannot prevent the generation of leachate. Therefore, in order to prevent the generation of leachate, steaming must be done very slowly or several times, which is undesirable.

When the present inventors put raw extradollar leaves in a sealed reaction container and heat-treat at high temperature under specific conditions, the extra-glycotic leaves do not burn even after high-temperature heat treatment due to the sufficient moisture contained in the raw extradollar leaves, and rather, the high glycoside saponin is sufficiently converted to low glycoside. The present invention was completed by confirming that the content of low molecular weight effective saponins such as ginsenoside Rg3, zipenoside L, zipenoside LI, damulin A and damulin B increased. In addition, according to the present invention, since benzopyrene formed in the process of high-temperature heat treatment of fresh peritoneum leaves is evaporated together with the water vapor contained in the fresh peritoneum leaves by releasing the pressure (seal) of the reaction vessel, it is possible to manufacture peritoneum leaf tea with reduced benzopyrene content. there is.

The low-molecular effective saponin can be sufficiently extracted from the leaf tea prepared according to the present invention even when extracted with ordinary room temperature water or alcohol, so the yield and low-molecular effective saponin content are significantly higher than that of general leaf tea prepared by roasting or steaming and then drying. high. In addition, as a result of the accelerated stability test of the leaf tea prepared according to an embodiment of the present invention, it was confirmed that the active ingredients were preserved much more stably than the general leaf tea prepared by roasting or steaming and then drying.

Specifically, the present invention includes the steps of (1) sealing after supplying rhizome leaves to a reaction container; (2) heating the inside of the reaction vessel at 112 to 150° C. for 10 minutes to 48 hours; and (3) releasing the pressure in the reaction vessel to dry the periwinkle leaves.

In the manufacturing method of ginguli leaf tea according to the present invention, the above step (1) is performed by supplying the ginguli leaf to the reaction container and then sealing it.

It is preferable that the leaves outside the stone are raw leaves outside the stone.

The fresh leaves outside the stone can be used for a storage period as short as possible after collection, preferably within 5 days, more preferably within 3 days, and most preferably, those that have just been collected are used as active ingredients by enzymes. loss is small.

The fresh leaves outside the stone are prepared by washing after collection and appropriately removing water. Residual moisture due to washing supplements moisture lost due to heat treatment, and the heating time can be shortened. However, if the residual moisture is too large, a large amount of leachate may occur during heat treatment, so care must be taken.

In the present invention, the reason for using the fresh ginguli leaves is that the conversion rate of the high glycoside saponin to the effective low-molecular saponin increases remarkably during the heat treatment process in the subsequent step (2) due to the sufficient moisture contained in the fresh silage leaves. You cannot achieve such an effect with

In one embodiment of the present invention, the fresh leaves outside the stone have a water content of 80% or more, preferably 85 to 95%, more preferably 90 to 95%.

In another embodiment of the present invention, the fresh peony leaves are put in an open container and heated to have a water content of 30 to 90% by weight, preferably 30 to 85% by weight, more preferably 40 to 85% by weight, More preferably, it may be adjusted to 50 to 85% by weight, more preferably 60 to 85% by weight. The reason for controlling the moisture content of the leaves is to inactivate the decomposing enzyme in the leaves, and to dry the excessive moisture remaining in the leaves to the extent that leachate does not occur during the heat treatment in step (2). It is for

If the moisture content of the gingulifolia is less than the lower limit, the conversion rate of the high glycoside saponin to the effective low molecular weight saponin may be lowered because sufficient deglycosylation hydrolysis reaction does not occur in the subsequent heat treatment in step (2). In this case, leachate may occur, and as a result, the effective saponin contained in the periwinkle leaf is eluted, and the content of the low-molecular effective saponin contained in the leaf tea may rather decrease. can

The heating temperature may be 60 to 300 °C, preferably 100 to 300 °C, more preferably 150 to 250 °C, and more preferably 150 to 180 °C.

When the heating temperature is less than the lower limit, enzyme inactivation may not occur and the heating time becomes longer, and when the heating temperature exceeds the upper limit, the amount of benzopyrene produced increases.

The method for the heating is not particularly limited as long as it can be heated, such as a method of heating by directly contacting the leaves on a heating plate or a method of heating using steam or the like.

Next, in step (2), the inside of the sealed reaction vessel is heated to 112 to 150 ° C, preferably 115 to 150 ° C, more preferably 120 to 150 ° C or 115 to 140 ° C, more preferably 120 to 140 ° C. °C or 115 to 130 °C, more preferably 120 to 130 °C for 10 minutes to 48 hours, preferably 10 minutes to 24 hours, more preferably 20 minutes to 12 hours, still more preferably 30 minutes to 8 hours , more preferably for 1 to 4 hours. By the heat treatment, the low-molecular-weight effective saponin precursors in the outer leaves are deglycosylated and hydrolyzed and converted into low-molecular-weight effective saponins. In addition, benzopyrene contained in the leaves generated when the open container is heated evaporates.

When the heating temperature is less than the lower limit, the conversion rate of the low molecular weight effective saponin precursors into the low molecular weight effective saponin decreases and the heating time becomes longer. When the heating temperature exceeds the upper limit, energy efficiency and safety problems may arise. decomposition, may cause an increase in benzopyrene.

When the heating temperature is increased, the heating time can be reduced, but as the heating temperature is increased, the pressure is increased and the amount of benzopyrene generated is also increased.

A specific example of the heat treatment is, when the heating temperature is 120 ° C, the heating time may be 1 to 48 hours, preferably 2 to 24 hours, and when the heating temperature is 130 ° C, the heating time is It may be 30 minutes to 12 hours, preferably 1 to 8 hours, and when the heating temperature is 140 ° C, the heating time may be 20 minutes to 8 hours, preferably 30 minutes to 2 hours, and the heating temperature is 150 In the case of ℃, the heating time may be 10 minutes to 4 hours, preferably 10 minutes to 1 hour.

Next, in step (3), the pressure of the reaction container is released to dry the periwinkle leaves. Since the reaction vessel is in a state where the temperature and pressure in the reaction vessel are significantly increased due to the heating in step (2), when the pressure in the reaction vessel is released, the water vapor in the reaction vessel rapidly evaporates and the leaves are dried under reduced pressure. occurs. At the same time, benzopyrene is also evaporated and discharged.

The outer leaf has a water content of less than 80% by weight, preferably 20 to 75% by weight, more preferably 40 to 75% by weight, more preferably 50 to 75% by weight, more preferably 60 to 70% by weight It becomes semi-dry.

The method for producing ginguli leaf tea of the present invention may be performed by adding (4) additionally drying the dried ginguli leaf tea after the step (3).

The drying is not limited in any way, such as hot air drying, freeze drying, vacuum drying, reduced pressure drying, or natural drying.

The drying may be carried out until the water content of the perilla leaf is less than 30% by weight, preferably 0.1 to 15% by weight, more preferably 2 to 15% by weight for storage stability.

In the above-mentioned method for manufacturing doluli leaf tea of the present invention, firstly, the reduction of active ingredients is minimized by immediately inactivating the decomposing enzyme contained in the fresh dolidone leaf, and second, heat treatment is performed using the moisture contained in the gilt leaf (steaming effect). ), the generation of benzopyrene is remarkably reduced as the leaves do not burn, and third, the leachate that may occur during the manufacturing process of the leaves is absorbed back into the leaves during the drying process, so there is little loss of active ingredients. Fourth, it is used for heat treatment. Energy consumption can be minimized by recycling the generated heat in the drying process, and finally, conversion to low-molecular-weight effective saponin can be maximized.

Ginsenoside leaf tea of the present invention prepared as described above contains 0.1 to 3.0 mg/g of ginsenoside Rg3, 0.5 to 12 mg/g of zipenoside L, and 0.5 to 8 mg/g of zipenoside LI based on the weight of solid content. g, 0.5 to 10 mg/g of damulin A and 0.5 to 10 mg/g of damulin B, and 10 ppb or less of benzopyrene (eg, within a range of 0.01 to 10 ppb).

More specifically, the ginseng leaf tea of the present invention contains 0.1 to 3.0 mg/g, preferably 0.5 to 2.5 mg/g, more preferably 1.0 to 2.0 mg/g of ginsenoside Rg3 based on the weight of the solid content, , 0.5 to 10 mg/g of zipenoside L, preferably 2 to 10 mg/g, more preferably 5 to 10 mg/g of zipenoside L, and 0.5 to 8 mg/g of zipenoside LI, 2 ~ 10 mg/g, more preferably 4 ~ 8 mg/g, and 0.5 ~ 10 mg/g, preferably 2 ~ 10 mg/g, more preferably 4 ~ 8 mg/g of Damulin A g, may contain damulin B at 0.5 to 10 mg/g, preferably 2 to 10 mg/g, more preferably 2 to 7 mg/g, and benzopyrene at 10 ppb or less, preferably is less than or equal to 5 ppb, more preferably 0.01 to 3 ppb, more preferably 0.1 to 2 ppb.

In addition, ginsenoside Rg3, zipenoside L, zipenoside LI, and other It is very useful because low-molecular effective saponins such as Mulin A and Damulin B are steeped in high content, the extraction yield is increased, and the benzopyrene content is low.

In addition, since the sedum leaf tea prepared according to the present invention is obtained by maximally converting the low-molecular-weight effective saponin precursor in the sedum leaf to the low-molecular-weight saponin, in the case of using the same amount of sedum leaf tea, one or more conditions selected from among solvent, pressure, and temperature It was confirmed through specific experiments that there was no significant difference in the content of the extracted low-molecular-weight effective saponin, even if the extraction was performed differently.

In addition, the present invention includes (1) supplying gynae leaves to a reaction container and then sealing it; (2) heating the inside of the reaction vessel at 112 to 150° C. for 10 minutes to 48 hours; and (3) performing atmospheric hot water extraction or ethanol extraction of the heated ginseng leaf tea at 40 to 100 ° C.;

For the “(1) Step of sealing after supplying Ginseng leaf to the reaction vessel” and “(2) Step of heating the inside of the reaction vessel at 112 to 150 ° C. for 10 minutes to 48 hours” of the present invention, Since it has already been described above in the description of the manufacturing method, the description thereof is omitted to avoid excessive duplication.

The present invention may additionally include (2-1) a step of drying the periwinkle leaf by releasing the pressure of the reaction vessel between the steps (2) and (3).

Since the "step of drying the leaves by releasing the pressure of the reaction vessel" used in the present invention has already been described in detail in the description of the method for manufacturing periwinkle leaves, description thereof will be omitted to avoid excessive duplication.

In addition, the present invention, between the step (2-1) and the step (3), (2-2) drying the outer leaf to a moisture content of 2 to 15% by weight; may additionally include .

Since the “step of drying the perilla leaves to have a moisture content of 2 to 15% by weight” used in the present invention has already been described in detail in the description of the manufacturing method of peridot leaf tea, description thereof will be omitted to avoid excessive duplication.

In the step (3), the peony leaf is subjected to atmospheric hot water extraction or ethanol extraction at 40 to 100 ° C, preferably 50 to 100 ° C, more preferably 70 to 100 ° C, and still more preferably 80 to 100 ° C. If the extraction temperature is less than the lower limit, the extraction efficiency of the low molecular weight effective saponin component may decrease, and if it exceeds the upper limit, the extraction efficiency is low compared to the effort and / or cost for increasing the temperature, so it is not economical.

Preferably, the extraction of step (3) is repeated twice, but the first extraction is performed by hot water extraction at 40 to 100 ° C., and the second extraction is performed by 40 to 80% (v / v) ethanol extraction. It is possible to increase the yield and increase the content of the saponin component in the extract of the leaf tea extract. In order to increase the saponin content of the ginseng leaf tea extract, an ethanol aqueous solution may be used as an extraction solvent for both the primary extraction and the secondary extraction, and the number of extractions may exceed two times, but is not preferable in terms of recovery rate and cost.

The content of low-molecular-weight effective saponins such as ginsenoside Rg3, zipenoside L, zipenoside LI, damulin A and damulin B without high-pressure treatment or high-temperature treatment is It is very useful because it has a high extraction yield and a low content of benzopyrene.

In addition, the ginseng leaf tea extract contains 0.5 to 7.0 mg/g of ginsenoside Rg3, 1.5 to 90 mg/g of zipenoside L, 1.5 to 80 mg/g of zipenoside LI, and 2.0 to 60 mg of damulin A. /g and damulin B are included at 2.0 to 60 mg/g, and benzopyrene is included at 10 ppb or less (eg, within the range of 0.01 to 10 ppb).

More specifically, the ginseng leaf tea extract contains ginsenoside Rg3 at 0.5 to 7.0 mg/g, preferably at 0.7 to 5.0 mg/g, more preferably at 0.9 to 4.0 mg/g, and even more preferably at 1.0 to 3.5 mg. You can include it with /g. In addition, 1.5 to 90 mg/g of zipenoside L, preferably 5.0 to 30 mg/g, more preferably 15 to 30 mg/g, and more preferably 20 to 30 mg/g may be included. . In addition, 1.5 to 80 mg/g of zipenoside LI, preferably 1.5 to 30 mg/g, more preferably 5.0 to 20 mg/g, and more preferably 10 to 20 mg/g may be included. . In addition, damulin A may be included in an amount of 2.0 to 60 mg/g, preferably 2.0 to 20 mg/g, more preferably 5.0 to 18 mg/g, and even more preferably 10 to 16 mg/g. In addition, damulin B may be included in an amount of 2.0 to 60 mg/g, preferably 2.0 to 18 mg/g, more preferably 5.0 to 18 mg/g, and even more preferably 8.0 to 16 mg/g.

The extract of the wild leaf tea may be prepared in a concentrated liquid or dry powder state. Specifically, the filtered ginseng leaf tea extract may be used after being concentrated to 60 to 70 brix. Alternatively, the above extract of ginseng leaf tea may be prepared in a powder state that has undergone an additional process such as vacuum drying, freeze drying, or spray drying.

The periwinkle leaf tea or the periwinkle leaf tea extract of the present invention has low benzopyrene content and is less harmful, and has an increased content of low-molecular saponins such as Rg3, zipenoside L, and zipenoside LI, thereby preventing, improving or treating diabetes or obesity. is excellent, and the effect of reducing muscle cell loss and improving exercise performance is excellent.

The present invention also provides a composition comprising the Oedophila leaf tea or the extract of the Oedophila leaf tea of the present invention as an active ingredient. In particular, the composition of the present invention can be used for preventing, improving, or treating obesity, diabetes, or muscle loss, and can be used to improve athletic performance. Depending on the method and purpose of use, the composition of the present invention may be a pharmaceutical composition, food composition, health food composition, quasi-drug composition, etc., but is not limited thereto.

In detail, the pharmaceutical composition of the present invention includes Oesophore leaf tea or an Oedea leaf tea extract as an active ingredient, and may further include a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier is one commonly used in formulation and includes, but is not limited to, saline solution, sterile water, Ringer's solution, buffered saline solution, cyclodextrin, dextrose solution, maltodextrin solution, glycerol, ethanol, liposome, etc. It is not, and if necessary, other conventional additives such as antioxidants and buffers may be further included. In addition, diluents, dispersants, surfactants, binders, lubricants, etc. may be additionally added to suitably form aqueous solutions, suspensions, fluids, and formulations according to each component. The pharmaceutical composition of the present invention is not particularly limited in formulation, but may be formulated as an injection, oral administration, external skin preparation, and the like.

The pharmaceutical composition may be administered orally or parenterally (for example, intravenously, subcutaneously, intraperitoneally or topically applied) depending on the desired method, and the dosage is the condition and weight of the patient, the severity of the disease, the drug It varies depending on the form, administration route and time, but can be appropriately selected by those skilled in the art.

The dosage level selected from the composition will also depend on the activity of the compound, the route of administration, the severity of the condition being treated and the condition and previous medical history of the patient being treated. However, it is within the knowledge of the art to start with a dose of the compound at a level lower than that required to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved, and the preferred dosage is age, sex and age. , body type, and weight can be determined. The composition may be further processed, preferably milled or ground into smaller particles, prior to formulation into pharmaceutically acceptable pharmaceutical preparations. The composition will also vary depending on the condition and the patient being treated, but this can be determined non-exclusively. For the desired effect, the effective dose of the Oxygen leaf tea or the extract of the Oxygen leaf tea of the present invention is 0.001 to 400 mg/kg, preferably 0.01 to 100 mg/kg, and may be administered 1 to 3 times a day. The dosage is not intended to limit the scope of the present invention in any way.

The pharmaceutical composition of the present invention is prepared in unit dosage form by formulation using a pharmaceutically acceptable carrier and/or excipient according to a method that can be easily performed by those skilled in the art. or it may be prepared by incorporating into a multi-dose container. At this time, the formulation can be used in any form suitable for pharmaceutical preparations, including oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols, external preparations such as ointments and creams, suppositories and sterile injection solutions, etc. , a dispersing agent or a stabilizing agent may be additionally included.

The present invention also provides a food composition for preventing or improving obesity or diabetes, or muscle loss, comprising the Oesopheles leaf tea or an extract of Oesophageal leaf tea as an active ingredient. The food composition of the present invention includes all forms such as functional food, health functional food, nutritional supplement, health food and food additives. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.

According to the present invention, in the composition, the content of the outer leaf tea or the outer leaf tea extract may be contained in 0.001 to 100% by weight, preferably 0.05 to 50% by weight, based on the total weight of the entire food composition. When the content of the Oedelaxa leaf tea or the Oedelaxa leaf tea extract is less than the above range, there is a problem in that it is difficult to expect the effect of the Oedelaxa leaf tea or the Oedelaxa leaf tea extract.

As a specific example of the food composition, the outer leaf tea or the outer leaf tea extract of the present invention can be prepared in the form of tea, juice or drink for drinking, or granulated, encapsulated, or powdered to be consumed in the form of a health functional food. . In addition, it can be prepared in the form of a composition by mixing the sedum leaf tea or sedum leaf tea extract of the present invention with a known substance or active ingredient known to have an anti-obesity effect, anti-diabetic effect, or muscle loss inhibitory effect. For example, the food composition of the present invention may further contain trace amounts of minerals, vitamins, saccharides, and components having known anti-obesity or anti-diabetic activity, muscle loss inhibitory effects, etc.

In addition, examples of the food composition include drinks, meat, sausages, bread, biscuits, rice cakes, chocolates, candies, snacks, pizza, ramen, other noodles, gums, dairy products including ice creams, various soups, etc. It includes all functional foods in the meaning.

In addition, the health functional food composition is characterized in that it is formulated with one selected from the group consisting of tablets, pills, powders, granules, powders, capsules and liquid formulations, including at least one of carriers, diluents, excipients, and additives .

The Oedlifolia tea or Oedea leaf tea extract of the present invention may be added to food as it is or used together with other foods or food ingredients, and may be appropriately used according to conventional methods. The mixing amount of the active ingredient can be suitably determined depending on the purpose of its use (for prevention or improvement). Generally, the amount of the food composition in the food may be 0.001 to 100% by weight, preferably 0.01 to 50% by weight, more preferably 0.1 to 30% by weight of the total food weight. However, in the case of long-term intake for the purpose of preventing and improving obesity or for health control, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient can be used in an amount above the above range. can

Hereinafter, an example will be described in detail with respect to the Oedophila leaf tea or the Oedophila leaf tea extract according to the present invention.

<Example>

Example 1: Preparation of doloeip tea by direct heat treatment of raw doloe leaves

(1) After harvesting 10 kg of fresh leaves outside the plant, washing with clear water and removing water.

(2) After that, the ginseng leaves were supplied to a water bath container inside an electric extractor (Kyungseo Machinery Co., Ltd., COSMOS-660) and sealed. After adjusting the temperature inside the electric extractor to 130 ℃, it was heated for 3 hours.

(3) After the heating is finished, slowly open the valve of the electric extractor (equipped with a condenser and pressure reducing pump) to discharge water vapor into the condenser, and when the internal temperature drops below 100 ℃, gradually operate the pressure reducing pump to It was allowed to dry under reduced pressure. In this process, some leachate was generated from the leaves, but all of them were reabsorbed into the tea by evaporation due to the latent heat inside the device. In addition, after the internal temperature of the electric water bath reached 70 ° C., drying was performed under reduced pressure while maintaining 70 ° C. through additional heating. When no more water was recovered from the condenser, 2,523 g of gypsy leaves with a moisture content of 67.5% by weight were recovered.

(4) The recovered Ginkgo biloba leaves were dried with hot air at 65 ° C to obtain 825 g of Ginkgo biloba tea having a moisture content of 7% by weight.

(5) 10 g of the above-mentioned peony leaf tea was finely ground with a blender, 100 ml of 70% (v/v) ethanol was added, extracted three times for 2 hours at 80 ° C., filtered, and dried, and then used as a sample for analysis of peony leaf tea. used

Example 2: Preparation of Oedesa leaf tea after heat drying

(1) After harvesting 10 kg of fresh leaves outside the plant, washing with clear water and removing water.

Thereafter, the fresh leaves (moisture content of the leaves: 91.8% by weight) were placed in an open container and heated at 150 ° C using an electric heater to obtain 4,985 g of leaves with a moisture content of 83.6% by weight without leachate. .

Steps (2) and (3) were carried out in the same manner as in Example 1, and 2,543 g of Ginseng leaf with a moisture content of 69.5% by weight was recovered.

Step (4) was also carried out in the same manner as in Example 1 to obtain 829 g of sedum leaf tea having a water content of 2.9%, and an analysis sample was prepared in the same manner as in (5) of Example 1.

Example 3: Manufacture of Ginseng leaf tea extract

1,200 ml of water was added to 100 g of Ginseng leaf tea obtained in step (4) of Examples 1 and 2, extracted at 90 ° C for 4 hours, and the extract was collected by filtering with a 20um filter, and then 50% v / v alcohol was added to the remaining residue. After adding 1,200 ml, extracting at 80 ° C for 2 hours and recovering through filtration, the two extracts were combined, concentrated under reduced pressure and lyophilized, and then powdered to recover 31.8 g and 30.9 g, respectively. Analysis of the extract Samples were used as samples (3-1 (Example of Oesophore leaf tea according to Example 1) and 3-2 (Extract of Oedea leaf tea according to Example 2), respectively).

<Method for analyzing effective small-molecular-weight saponin>

In order to measure the content of active ingredients in the extracts of the Oesophore leaf tea according to Examples 1 and 2 and Examples 3-1 and 3-2, HPLC analysis was performed under the conditions shown in Table 1 below, and The results are shown in Table 2 below.

(1) Preparation of standard stock solution: About 20 mg of the standard material was precisely weighed and dissolved in a 20 mL constant-use flask with 60% methanol. (Stock solution: 1,000 ppm)

(2) Preparation of standard solution: 0.3, 0.6, 1.2, 2.0, 3.0 mL of each standard stock solution was put into a 10 mL constant-use flask and used as a standard solution by aligning the marked line. (30, 60, 120, 200, 300 ppm)

(3) Preparation of test solution: 500 mg of the sample was weighed and placed in a 50 mL constant-use flask, half filled with 60% methanol, and ultrasonically extracted for 30 minutes. After cooling at room temperature, it was adjusted to the marked line with 60% methanol. The test solution was filtered through a 0.45 μm Nylon syringe filter.

(4) The instrumental analysis conditions are shown in Table 1 below, and the analysis results are shown in Table 2 below.

The standards used in the experiment were Damulin A (99.1% pure) and Damulin B (95.96% pure) from Chengdu Biopurify Phytochemicals (China), and Gypenoside L (99.42% pure) from Shanghai yuanye Bio-Technology (China). , Gypenoside LI (99.33% purity) was purchased from Korea Research Institute of Bioscience and Biotechnology, and Gynsenoside Rg3 (98.24% purity) was purchased from Ambo (Korea).

Analytical instrument HPLC-DAD detection wavelength 204 nm column Agilent ZORBAX Eclipse C18 (4.6*250mm, 5μm particle size) column temperature 35 ℃ injection volume 10 µL flow rate 1.0 mL/min mobile phase Solvent A: DW, Solvent B: Acetonitrile Time (min) Solvent A (%) Solvent B (%) 0 60 40 20 60 40 35 50 50 40 50 50 40.1 0 100 50 0 100 50.1 60 40 60 60 40

Classification (mg/g) Gyphenoside L Gyphenoside LI Rg3 closed A closed B Benzopyrene
(ppb) Example 1
(exophytic leaf) 8.11 5.12 1.01 5.01 4.28 0.5 Example 2
(Heat treatment and drying of exogenous leaves) 8.29 5.23 1.18 5.61 4.32 5.5 Example 3-1
(Example 1 extract) 23.05 13.87 2.59 12.77 11.19 1.8 Example 3-2
(Example 2 extract) 22.75 13.81 2.63 12.98 11.23 6.8

As shown in Table 2, Example 2 of the present invention was found to have a higher content of low molecular weight effective saponin than Example 1. However, Example 2 showed that the benzopyrene content was relatively high due to the heat treatment of the drying process before the heat treatment of the fresh leaves.

On the other hand, in the case of the extracts of Ginkgo biloba of Examples 3-1 and 3-2, a filter size of 20 μm was used, and no separate purification process such as centrifugation, solvent separation, or column separation was performed. Since the content of the saponins can be increased by several to several tens of times when sizing, appropriate centrifugation and solvent separation, column separation, etc. are performed, the content is not limited to the content presented in this example.

Test Example 1. Change in active ingredient content according to heating temperature

Prepared by heating for 6 hours at 100 ℃, 110 ℃, 115 ℃, 121 ℃, 130 ℃, 150 ℃, respectively, in order to confirm the change in the active ingredient content according to the heating temperature in step (2) of Example 3-2 The content of the active ingredient contained in the extract of the leaf tea was measured, and the results are shown in Table 3 below.

Classification (mg/g) Gyphenoside L Gyphenoside LI Rg3 closed A closed B 100℃ 0.00 0.00 0.00 0.01 0.00 110℃ 0.01 0.09 0.00 0.01 0.00 115℃ 6.71 4.78 0.98 4.89 4.21 121℃ 24.98 13.82 2.48 14.91 11.28 130℃ 19.45 12.49 2.13 12.82 10.47 150℃ 17.83 11.21 2.06 8.82 6.43

Looking at Table 3, when the heating temperature was 100 ° C and 110 ° C, there was no significant change in the content of effective low-molecular-weight saponin contained in the leaves, and it was confirmed that a significant change appeared from 115 ° C or higher. Therefore, it is assumed that the critical temperature is between 110 and 115 °C. On the other hand, it can be seen that the content of low-molecular-weight effective saponin peaks when heated to 121 ° C., and then gradually decreases as the temperature increases when the temperature exceeds 121 ° C. Since the temperature and the heating time are inversely proportional, increasing the heating temperature requires a shorter heat treatment, but heating for too long is interpreted as a result of decomposition of the active ingredient.

From the above results, it can be seen that the content of low-molecular-weight effective saponin in the leaf tea prepared according to the present invention was significantly increased compared to the leaf tea prepared by heating at 110 ° C or lower. Particularly, it can be seen that the content of low-molecular-weight effective saponin is high when the sedum leaf tea prepared according to the present invention is prepared by heating at preferably 120 to 150 ° C, more preferably 120 to 130 ° C.

Test Example 2. Change in active ingredient content according to heating time

In order to confirm the change in active ingredient content according to the heating time in step (2) of Example 3-2, heating was performed at 130 ° C. for 0, 1, 2, 3, 4, 6, 8, 12 and 24 hours, respectively. The content of the active ingredient contained in each of the prepared extracts of Spermiana leaf tea was measured, and the results are shown in Table 4 below.

Classification (mg/g) Gyphenoside L Gyphenoside LI Rg3 closed A closed B Benzopyrene
(ppb) 0 hours 0.00 0.00 0.00 0.00 0.00 0.45 1 hours 8.55 5.47 1.21 5.27 4.83 0.49 2 hours 18.65 14.15 2.15 10.53 8.48 0.82 3 hours 25.05 13.92 2.65 15.05 11.42 1.71 4 hours 22.05 12.32 2.35 12.01 9.42 2.74 6 hours 19.45 12.49 2.13 12.82 10.47 2.82 8 hours 18.20 11.16 1.23 10.15 8.14 3.65 12 hours 16.03 9.23 1.09 8.41 6.33 4.83 24 hours 14.35 8.72 1.08 6.31 4.34 9.31

Looking at Table 4, the low-molecular-weight effective saponin content began to increase after 1 hour of heat treatment in step (2), significantly increased after 2 hours, and peaked at 3 to 4 hours. It can be seen that it gradually decreases after 6 hours after taking . The decrease in the content of effective low-molecular-weight saponin as the heat treatment time increases is presumed to be because the effective low-molecular-weight saponin is converted into a more low-molecular-weighted form or the saponin is decomposed by long-term high-temperature treatment.

Therefore, the Ginseng leaf tea containing a high content of effective low-molecular-weight saponin is prepared by heating in step (2) for 2 to 12 hours, preferably 2 to 6 hours, more preferably 3 to 6 hours, and most preferably 3 hours. It can be confirmed that it was prepared by heating for 4 hours.

On the other hand, in the case of benzopyrene, it can be confirmed that the content increases as the heating time increases.

Comparative Example 1: Natural Dried Peony Leaves

Dried leaves were obtained by spreading the raw leaves in the shade and naturally drying them to a moisture content of 7%.

After extracting the above-mentioned ginseng leaf tea twice with 80 ° C. alcohol (50% v/v) corresponding to 15 times the weight, the extract was prepared, concentrated under reduced pressure and lyophilized to pulverize, and then used as an analysis sample in the experiment. did

Comparative Example 2: Dried rhododendron leaves after roasting

After roasting the raw leaves of doloeia using an electric heater at 130 ° C. for 30 minutes, they were dried to a moisture content of 7% to prepare gilded leaf tea.

After extracting the above-mentioned ginseng leaf tea twice with 80 ° C. alcohol (50% v/v) corresponding to 15 times the weight, the extract was prepared, concentrated under reduced pressure and lyophilized to pulverize, and then used as an analysis sample in the experiment. did

Comparative Example 3: Natural Dried Ginkgo Leaf Extract - Heat Treatment

Dried leaves were obtained by spreading the raw leaves in the shade and naturally drying them to a moisture content of 7%.

The dried doloe leaf was extracted twice with 80 ° C. alcohol (50% v / v) corresponding to 15 times the weight to prepare a doloe leaf tea extract.

After concentrating the extract of Ginseng leaf to a solid content of 22% by weight, the mixture was heated at 121 ° C. for 4 hours.

The heated ginseng leaf extract was concentrated under reduced pressure and lyophilized, and then powdered and used as a sample for analysis of the ginseng leaf tea extract in subsequent experiments.

Comparative Example 4: Dried Ginkgo biloba leaf extract after roasting - heat treatment

After roasting the raw leaves of doloeia using an electric heater at 130 ° C. for 30 minutes, they were dried to a moisture content of 7% to prepare gilded leaf tea.

The Oedeol leaf tea was extracted twice with 80 ° C. alcohol (50% v / v) corresponding to 15 times the weight to prepare an Oede leaf tea extract.

After concentrating the extract of Ginseng leaf to a solid content of 22% by weight, the mixture was heated at 121 ° C. for 4 hours.

The heated ginseng leaf extract was concentrated under reduced pressure and lyophilized, and then powdered and used as a sample for analysis of the ginseng leaf tea extract in subsequent experiments.

Test Example 3. Analysis of Active Ingredient Content of Ginseng Leaf Tea According to Manufacturing Method

Using the analysis samples according to Example 3-2 and Comparative Examples 1 to 4, the content of low-molecular-weight effective saponin contained in each sample was measured in the same manner as in Test Example 1, and shown in Table 5 below.

Classification (mg/g) Gyphenoside L Gyphenoside LI Rg3 closed A closed B Benzopyrene
(ppb) Example 3-2 22.75 13.81 2.63 12.98 11.23 1.8 Comparative Example 1 0.00 0.00 0.00 0.00 0.00 1.2 Comparative Example 2 1.3 0.9. 0.1 0.7 0.5 21.5 Comparative Example 3 6.3 4.2 0.3 2.3 1.8 10.5 Comparative Example 4 18.2 14.3 1.6 8.2 6.1 23.5

Referring to Table 5, it was confirmed that the extract of Oesophageal japonica leaf tea according to Example 3-2 of the present invention had a significantly higher content of effective low-molecular-weight saponin and a lower content of benzopyrene than Comparative Examples 1 to 3.

Specifically, in the case of Comparative Example 1, which was naturally dried without heat treatment, the content of low molecular weight effective saponin was below the detection limit or the content was very low. In addition, in the case of Comparative Example 3, in which the extract of naturally dried ginseng leaf tea was heat-treated, it is assumed that the effective saponin component was lost due to the action of enzymes during natural drying, as the low molecular weight effective saponin content was low.

In addition, it can be confirmed that the extract of ginseng leaf tea according to Example 3-2 of the present invention has a higher content of low-molecular saponin and a much lower benzopyrene content than Comparative Example 4.

Test Example 4. Accelerated Stability Test

An accelerated stability test was conducted on each of the Oedophila leaf tea powder and the Oedophila leaf tea extract powder according to the embodiment of the present invention.

First, after pulverizing the peony leaf tea obtained in step (4) of Example 1 to 200 mesh or less, 2 g each was put in an aluminum foil bag and sealed. Each bag containing the sample was stored for 0, 1, 2, 3, 4, 6, 8, and 12 months, respectively, at a temperature of 40 °C and a relative humidity of 70%. Thereafter, each doloeip tea was opened and the content of the active ingredient was analyzed whenever a certain period of time elapsed.

In addition, after storage of the extract powder of ginseng leaf tea according to Example 3-2 in the same manner as above, it was opened and the active ingredient content was measured.

Table 6 shows the results of the accelerated stability test of the Oedlifolia tea powder and the Oedea leaf tea extract powder. Table 6 shows the total content of 5 kinds of low-molecular-weight effective saponins (ginsenoside Rg3, zipenoside L, zipenoside LI, damulin A and damulin B) contained in each of the sedum leaf tea powder or the sedum leaf tea extract powder. was expressed as a relative ratio (%) to the initial value.

Storage period Example 1 (%) Example 3-2 (%) 0 months 100 100 1 month 100.02 99.89 2 months 99.92 99.15 3 months 99.13 98.19 4 months 98.97 97.38 6 months 98.34 96.61 8 months 98.11 94.26 12 months 98.07 91.37

Looking at Table 6, it was confirmed that both the Oesophila leaf tea powder and the Oesophila leaf tea extract powder according to the embodiment of the present invention maintained 90% or more of effective low-molecular-weight saponin content up to 12 months under the accelerated test conditions. In addition, it can be seen that the change in the content of the active ingredient occurs very slowly compared to the extract of the Oedelae leaf tea when stored as the Oedlifolia tea.

<Production Example>

Preparation Example 1: Preparation of tablets

200 mg of the extract powder of the silage leaf tea of Example 1 or the extract powder of the silage leaf tea of Example 2, 9 mg of vitamin E, 9 mg of vitamin C, 200 mg of galactooligosaccharide, 60 mg of lactose, and 140 mg of maltose were mixed and granulated using a fluidized bed dryer. After that, 6 mg of sugar ester is added. Tablets are prepared by compressing 500 mg of these compositions in a conventional manner.

Preparation Example 2: Preparation of capsules

According to the conventional method for preparing soft capsules, 200 mg of the Oesophyte tea powder of Example 1 or the extract powder of the Oedelaxa leaf tea of Example 2, 9 mg of vitamin C, 2 mg of palm oil, 8 mg of hydrogenated vegetable oil, 4 mg of yellow wax, and 9 mg of lecithin were mixed and gelatin capsules were mixed. Filled in to prepare soft capsules.

Preparation Example 3: Preparation of Ginseng leaf tea powder

After finely pulverizing the peony leaf tea of Example 1 using a pin mill, a powder passed through a 300 mesh sieve was obtained.

Preparation Example 4: Preparation of Pills

[0080] The ginguli leaf tea of Example 1 was pulverized to obtain a powder passing through 200 mesh. Pills were prepared after suitably kneading honey, dextrin, starch, microcrystalline cellulose, CMC calcium, etc. with the above-mentioned stone leaf tea powder.

Preparation Example 5: Preparation of concentrated liquid tea

Water was added to the powder of the extract of the extract of Pedophilia leaf tea of Example 3-2 to dilute it into a mixed solution having a solid content of 15%. After heating this at 90 ° C., 10 parts by weight of γ-Cyclodextrin was added to and mixed with respect to 100 parts by weight of the extract powder of Ginseng leaf tea. The mixture was concentrated to 60% to prepare a concentrated liquid tea.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention can be embodied in other specific forms without changing its technical spirit or essential features. In this regard, the embodiments described above should be understood as illustrative in all respects and not limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later and equivalent concepts rather than the detailed description above are included in the scope of the present invention.

Claims (15)

(1) sealing the reaction container after supplying the leaves;
(2) heating the inside of the reaction vessel at 112 to 150° C. for 10 minutes to 48 hours; and
(3) releasing the pressure in the reaction vessel to dry the periwinkle leaves; According to claim 1,
(1) A method for producing gilt leaf tea, characterized in that the gilt leaf is a fresh leaf with a moisture content of 80% by weight or more. According to claim 1,
(1) A method for producing gilt leaf tea, characterized in that the moisture content is adjusted to 30 to 90% by weight by putting the fresh dol leaf in an open container and heating it. According to claim 3,
The heating is a method for producing stone leaf tea, characterized in that carried out at 60 to 300 ℃. According to claim 1,
(4) the step of drying the ginguli leaves to a moisture content of 2 to 15% by weight; According to claim 1,
The ginseng leaf tea contains 0.1 to 3.0 mg/g of ginsenoside Rg3, 0.5 to 12 mg/g of zipenoside L, 0.5 to 8 mg/g of zipenoside LI, and 0.5 mg/g of damulin A, based on solid weight. ~ 10 mg / g and 0.5 ~ 10 mg / g of damulin B, and benzopyrene of 10 ppb or less. It is prepared according to the method of any one of claims 1 to 6, and contains 0.1 to 3.0 mg/g of ginsenoside Rg3, 0.5 to 12 mg/g of zipenoside L, and zipenoside LI based on solid weight. 0.5 to 8 mg/g, 0.5 to 10 mg/g of damulin A and 0.5 to 10 mg/g of damulin B, and benzopyrene at 10 ppb or less. (1) sealing the reaction container after supplying the leaves;
(2) heating the inside of the reaction vessel at 112 to 150° C. for 10 minutes to 48 hours; and
(3) extracting the ginguli leaf with hot water or ethanol under normal pressure at 40 to 100 ° C. According to claim 8,
(1) A method for producing an extract of Oedeol leaf tea, characterized in that the leaves of the step (1) are raw leaves having a moisture content of 80% by weight or more. According to claim 8,
(1) A method for producing an extract of doloe leaf tea, characterized in that the moisture content of the doloe leaf is adjusted to 30 to 90% by weight by putting the fresh dol dol leaf in an open container and heating it. According to claim 10,
The heating is a method for producing a peony leaf tea extract, characterized in that carried out at 60 to 300 ℃. According to claim 8,
Between steps (2) and (3),
(2-1) the step of releasing the pressure in the reaction vessel to dry the periwinkle leaves; a method for producing an extract of periwinkle leaf tea, characterized in that it additionally comprises. According to claim 12,
Between steps (2-1) and (3),
(2-2) drying the periwinkle leaves to a moisture content of 2 to 15% by weight. According to claim 8,
The ginseng leaf tea extract contains 0.5 to 7.0 mg/g of ginsenoside Rg3, 1.5 to 90 mg/g of zipenoside L, 1.5 to 80 mg/g of zipenoside LI, and 2.0 to 60 mg/g of damulin A. g and damulin B in an amount of 2.0 to 60 mg/g, and benzopyrene in an amount of 10 ppb or less. It is prepared by any one method selected from claims 8 to 13,
0.5 to 7.0 mg/g of ginsenoside Rg3, 1.5 to 90 mg/g of zipenoside L, 1.5 to 80 mg/g of zipenoside LI, 2.0 to 60 mg/g of damulin A and damulin B 2.0 to 60 mg/g, and benzopyrene at 10 ppb or less.

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