KR20230026882A - Hair cosmetic composition comprising small molecules derived from silkworn hemolymph for protecting hair and repairing hair damage - Google Patents

Abstract

The present invention relates to a cosmetic composition for hair protection and restoration containing small molecules derived from silkworm hemolymph and a method for improving hair quality. The combined treatment can protect hair more effectively from hair damage that occurs during iron permanent treatment than single treatment of silkworm hemolymph-derived small molecules and castor oil. Therefore, the complex of silkworm hemolymph-derived small molecule and castor oil can be usefully used to protect hair from hair damage and restore damaged hair during permanent treatment.

Description

Cosmetic composition for hair protection and restoration containing small molecules derived from silkworm hemolymph

The present invention relates to a cosmetic composition for hair protection and restoration containing a small molecule derived from silkworm hemolymph and a method for improving hair quality.

The cuticle layer of hair is made of a protein called keratin, and the elasticity of hair is maintained through disulfide bonds (-S-S-) between component cysteines. Chemical and physical stimuli, such as permanents, weaken or break these disulfide bonds, thereby reducing the strength of the hair. Due to such damage to the hair, the bond of the protein constituting the hair is broken, which not only loses its luster, but also makes it difficult to comb due to tangles, and becomes frizzy hair because it does not moisturize. In particular, permanent treatment after dyeing or bleaching creates an unrecoverable hair environment.

As interest in hair styles increases, demand for products capable of preventing or improving hair damage increases, and research on hair care methods is steadily progressing. In particular, studies on naturally derived materials such as plant extracts, herbal materials, and animal proteins are being actively conducted. However, there is a limit to the products developed so far to be applied according to various hair styling treatment methods, so it is necessary to develop a hair protection substance that can more fundamentally solve the cause of damage and maintain healthy hair in the long term.

Silkworm hemolymph is silkworm hemolymph, a solution that nourishes the silkworm itself. Such hemolymph contains nutrients necessary for the growth of animal cells including insect cells. In addition, silkworm bodily fluid has high utility value in the development of natural materials, such as obtaining approval for stability in items such as endotoxin, and research to utilize it is actively underway. Korean Patent Publication No. 10-1501544 discloses antioxidant, antibacterial, and whitening effects of a 70 KDa protein derived from silkworm hemolymph, and Korean Patent Publication No. 10-2011-0105627 discloses a 30 KDa protein group derived from silkworm hemolymph The anti-obesity effect of was disclosed. However, until now, there is no known hair protection effect of the silkworm hemolymph-derived low-molecular-weight substance, and thus, research on the effect is required.

KR 10-1501554 B1 KR 10-2011-0105627 A

Accordingly, the present inventors studied a method for preventing hair damage that occurs during iron permanent treatment. As a result, it was confirmed that low molecular weight derived from silkworm hemolymph and castor oil protect hair from hair damage that occurs during iron permanent treatment and increase permanent efficiency. completed the present invention.

In order to achieve the above object, one aspect of the present invention provides a cosmetic composition for hair protection and restoration comprising a small molecule derived from silkworm hemolymph as an active ingredient.

Another aspect of the present invention provides a permanent wave composition comprising a low molecule derived from silkworm hemolymph and castor oil.

Another aspect of the present invention provides a permanent wave kit comprising the cosmetic composition for protecting and restoring hair, a first agent as a reducing agent, and a second agent as an oxidizing agent.

Another aspect of the present invention is to treat hair with low molecular weight derived from silkworm hemolymph and castor oil; And it provides a cosmetic method for improving the permanent efficiency and hair damage comprising the step of performing a permanent on the hair.

In the present invention, in order to confirm the improvement effect on hair damage that occurs during the iron permanent treatment, natural hair, bleaching once, bleaching twice, or bleaching three times are performed to castor oil, silkworm hemolymph low molecular weight, or silkworm The hair protection effect of the combined treatment of hemolymph small molecule and castor oil was comparatively analyzed. As a result, the highest hair protection effect was confirmed in the combined treatment of silkworm hemolymph low molecular weight and castor oil in the analysis results of sulfur content, wave efficiency, epidermal adhesion, tensile strength, elongation, and moisturizing power. Next, the hair protection effect was confirmed in the order of silkworm hemolymph small molecule, castor oil, and untreated treatment group. Through the above results, it was confirmed that the combined treatment can more effectively protect hair from hair damage that occurs during the iron permanent treatment than the single treatment of silkworm hemolymph small molecule and castor oil. Therefore, the silkworm hemolymph low molecule and castor oil complex can be usefully used to protect hair from hair damage during permanent treatment and to restore damaged hair.

1 is a diagram showing a hair structure.
2 is a view showing an example of castor oil.
3 is a diagram schematically showing a manufacturing process of a low molecular weight substance derived from silkworm hemolymph.
4 is a diagram showing the results of measuring the molecular weight of small molecules derived from silkworm hemolymph using a liquid chromatograph mass spectrometer.
5 is a schematic diagram showing an experimental process.
6 is a schematic diagram showing an iron permanent treatment model.
7 is an experimental apparatus used in the experiment, which includes an energy dispersive X-ray spectrometer (a), a universal testing machine (b), a dryer (c), an iron (d), and a digital caliper (e). ) is a drawing showing.
8 is a view showing the results of observing the hair condition of the untreated group with a scanning microscope after applying damage to natural hair, bleaching once, bleaching twice, or bleaching three times.
9 is a view showing the results of observation with a scanning microscope of the change in hair condition according to the treatment with a small molecule derived from silkworm hemolymph after damaging the hair by performing bleaching once, twice, or three times on natural hair. .
10 is a view showing the results of observation with a scanning microscope of changes in hair condition according to castor oil treatment after damage to natural hair, bleaching hair once, bleaching twice, or bleaching hair three times.
Figure 11 shows the change in hair condition according to the combined treatment of silkworm hemolymph-derived low-molecular weight and castor oil after damage to the hair by carrying out natural hair, bleaching once, bleaching twice, or bleaching three times It is a drawing showing the results observed with a scanning microscope.
Figure 12 shows the cysteine sulfur (S) component contained in the hair of untreated (a), castor oil (b), silkworm hemolymph-derived small molecule (c), or silkworm hemolymph-derived small molecule and castor oil combined treatment (d) treatment group This is a diagram showing the results of the analysis.
13 shows natural hair, untreated (a), castor oil (b), low molecule derived from silkworm hemolymph (c), or silkworm after damage to hair by bleaching once, twice, or bleaching three times. It is a graph showing the tensile strain (%) and tensile stress (MPa) of the hemolymph-derived small molecule and castor oil combination treatment group (d).
14 is a diagram showing the results of measuring the wave efficiency of untreated, castor oil, silkworm hemolymph-derived small molecule, or silkworm hemolymph-derived small molecule and castor oil treatment groups in natural hair.
15 is a diagram showing the results of measuring the wave efficiency of untreated, castor oil, small molecules derived from silkworm hemolymph, or combined treatment groups with low molecules derived from silkworm hemolymph and castor oil after damaging hair with one bleaching operation. .
16 is a diagram showing the results of measuring the wave efficiency of untreated, castor oil, small molecules derived from silkworm hemolymph, or combined treatment groups with low molecules derived from silkworm hemolymph and castor oil after damaging hair by bleaching twice. .
17 is a diagram showing the results of measuring the wave efficiency of untreated, castor oil, small molecule derived from silkworm hemolymph, or combined treatment group with low molecule derived from silkworm hemolymph and castor oil after damaging the hair by bleaching three times. .

Cosmetic composition for hair protection and restoration

One aspect of the present invention provides a cosmetic composition for hair protection and restoration comprising a small molecule derived from silkworm hemolymph as an active ingredient. In this case, the silkworm hemolymph-derived small molecule may be a low molecular weight material of about 10 KDa or less.

Silkworm refers to the larvae of the silkworm moth belonging to the Lepidoptera Silkworm family. The silkworm grows while eating mulberry leaves and sleeps in the 4th instar, and when it reaches the 5th instar, it grows rapidly to about 8 cm. .

As used herein, the term "silkworn hemolymph" is a mixture of silkworm blood and lymph fluid, and is a solution containing nutrients necessary for the growth of silkworms. Unlike common silkworm powder or silkworm extract, About 70 KDa protein group, which is known to have antioxidant, antibacterial and whitening effects, and about 30 KDa protein group, which is known to have an effect of inhibiting apoptosis of higher cells, are known as low-molecular-weight proteins of silkworm hemolymph.

The term "small molecule" refers to an organic compound having a molecular weight of 900 Daltons (Da) in the fields of molecular biology and pharmacology. Small molecules often act as regulators in biological processes, and in particular, can bind to biopolymers such as proteins or nucleic acids to regulate the functions of biopolymers. For example, it may be a compound, oligonucleotide, oligopeptide, nucleic acid, amino acid, etc., but is not limited thereto.

In the present invention, the small molecule has a molecular weight of about 10 KDa or less, about 9 KDa or less, about 8 KDa or less, about 7 KDa or less, about 6 KDa or less, about 5 KDa or less, about 4 KDa or less, about 3 KDa or less, or about 2 KDa or less. , about 1 KDa or less, about 900 Da or less, about 800 Da or less, about 700 Da or less, about 600 Da or less, about 500 Da or less, about 400 Da or less, about 300 Da or less, about 200 Da or less, or about 100 Da It may be the following organic compounds.

As used herein, the term "small molecule derived from silkworm hemolymph" refers to a small molecule obtained from silkworm hemolymph, and may be used interchangeably with "small molecule from silkworm hemolymph". The small molecule derived from silkworm hemolymph may have a molecular weight of about 10 KDa or less. More specifically, the molecular weight is about 1 KDa or less, about 900 or less, about 800 Da or less, about 700 Da or less, about 600 Da or less, about 500 Da or less, about 400 Da or less, about 300 Da or less, about 200 Da or less, or It may be about 100 Da or less. Preferably, it may be about 258 Da to about 589 Da. The small molecules derived from silkworm hemolymph of the present invention may be flavonoids, polysaccharides, proteins, lipids, glycoproteins, and glycolipids. Specifically, it may be a protein consisting of 2 to 5 amino acids, but is not limited thereto. The silkworm hemolymph-derived small molecule may have the molecular weight pattern shown in the upper part of FIG. 4 .

At this time, the cosmetic composition may contain about 0.001% by weight to about 20% by weight of the small molecule derived from silkworm hemolymph, which is an active ingredient, based on the total weight of the composition. Specifically, it may be about 0.001 wt% to about 0.1 wt%, about 0.01 wt% to about 1 wt%, about 0.1 wt% to about 10 wt%, or about 1 wt% to about 20 wt%. Preferably, it may contain about 0.1% by weight to about 10% by weight.

In addition, the cosmetic composition of the present invention may further include castor oil.

As used herein, the term "castor oil", also known as castor oil, is a fatty oil obtained by pressing castor seeds. Castor oil is characterized by its low freezing point, high boiling point (about 280 ° C) and non-toxicity. In Indian traditional medicine, castor oil is used for pain relief and anti-inflammatory, and its antibacterial effect is also known. In particular, castor oil is known to have high affinity for hair, play a role in removing inflammation or toxicity of the scalp, and have high moisturizing properties. Therefore, in the present invention, castor oil is used for hair protection and restoration that can protect hair from hair damage due to heat generated during permanent treatment.

The cosmetic composition may include about 0.001% by weight to about 20% by weight of the castor oil as an active ingredient, based on the total weight of the composition. Specifically, it may be about 0.001 wt% to about 0.1 wt%, about 0.01 wt% to about 1 wt%, about 0.1 wt% to about 10 wt%, or about 1 wt% to about 20 wt%. Preferably, it may be contained in 0.1% by weight to 10% by weight.

The cosmetic composition may be in the form of a mixture in which an active ingredient, a small molecule derived from silkworm hemolymph, and castor oil are mixed, or may be in the form of a single substance that is not mixed. When the composition is in the form of a mixture, the mixing ratio of the low molecule derived from silkworm hemolymph and castor oil is about 1:10 to about 10:1, about 1:9 to about 9:1, about 1:8 to about 8:1, about 1:7 to about 7:1, about 1:6 to about 6:1, about 1:5 to about 5:1, about 1:4 to about 4:1, about 1:3 to about 3:1, It may be a weight ratio of about 1:2 to about 2:1, or about 1:1. preferably about 2.5:1.

The cosmetic composition of the present invention can improve hair damage caused by permanent. At this time, the hair damage may represent a lifting phenomenon of the hair epidermis, loss of hair internodes, reduction in tensile strength or elongation of hair, reduction in hair moisturizing power, or a combination thereof.

In addition, the cosmetic composition of the present invention can increase the permanent efficiency of damaged hair.

As used herein, the term "permanent" refers to a method for modifying the protein cross-linked structure of natural hair using physical and chemical methods as a means for styling hair. Permanent types are largely divided into cold permanent and heat permanent. Cold permanent is a winding treatment method in which chemicals are applied to the hair and then wrapped around a rod (physical treatment), and heat permanent is applied after softening and chemicals are washed off, and heat treatment and physical treatment are performed using hot tools such as iron, digital, and press. It is a method of treatment in which The shape is fixed using two types of permanent oxidizing agents, and the design is completed with a wave shape appearing according to the shape and thickness of the rod. In the present invention, the permanent may be a cold permanent or a thermal permanent, preferably a thermal permanent.

According to one embodiment of the present invention, the pretreatment of the silkworm hemolymph-derived small molecule, castor oil, or the silkworm hemolymph-derived small molecule and castor oil increases the epidermal adhesion of the hair during permanent treatment using heat treatment, thereby preventing loss of hair parasites and increased the tensile strength and moisturizing power of the hair.

As used herein, the term "protection" refers to any action that inhibits or delays the occurrence of hair damage by treatment with the composition. As used herein, the term "restoration" refers to all activities that improve or beneficially change the condition of hair damage by treatment with the composition. Specifically, it includes healing, alleviation, prevention, prevention, delay, or reduction of hair damage caused by permanent.

Ingredients included in the cosmetic composition of the present invention may include additive components commonly used in hair cosmetic compositions in addition to silkworm hemolymph-derived low molecular weight and castor oil as active ingredients, and may include, for example, adjuvants.

Commonly used auxiliaries include stabilizers, solubilizers, vitamins, pigments, fragrances, surfactants, oil substances, emulsifiers, auxiliary emulsifiers, superfatting agents, pearlescent waxes, thickeners, thickening agents, fats, waxes, Silicone compounds, hydrotropic agents, preservatives, aromatic oils, dyes, stabilizers, pH adjusting agents, protective substances such as panthenol, collagen, vitamins, protein substances, solubilizers, complexing agents, etc. may be used, but Not limited.

The formulation of the composition of the present invention is of a property that can be applied to hair or scalp, etc., and is not particularly limited as long as it shows the effect of the present invention. For example, it may be formulated into solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, sprays, and the like. .

More specifically, hair conditioner, hair tonic, hair cream, hair lotion, hair face, hair gel, hair pack, hair massage, hair liquid, hair spray, hair mousse, treatment, non aerosol mousse, non aerosol spray, aerosol mousse , Aerosol spray, perm agent, bleach, shampoo, rinse, shampoo and rinse mixed type, soap, water dispersion, powder, or may be prepared in the formulation of oil, but is not limited thereto.

Ingredients included in the cosmetic composition of the present invention may include carriers commonly used in cosmetic compositions in addition to low molecules derived from silkworm hemolymph and castor oil as active ingredients.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. are used as carrier components. It may be, but is not limited thereto.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additionally chlorofluorohydrocarbon, propellants such as, but not limited to, propane/butane, or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, fatty acid esters of glycerol, polyethylene glycol, or fatty acid esters of sorbitan, but are not limited thereto.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol, or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracanth may be used, but is not limited thereto.

When the formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether Sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used, but is not limited thereto.

The treatment method of the cosmetic composition according to the present invention is not particularly limited as long as it exhibits the effects of the present invention. Alternatively, it may be treated by a method such as directly applying or spraying to the hair or scalp, but is not limited thereto. The amount of use of the composition of the present invention should be appropriately determined according to individual differences such as hair condition and treatment conditions, or the formulation and form of the composition.

In addition, the cosmetic composition of the present invention can be used alone or in combination with other conventional hair protecting agents, and can be processed sequentially or simultaneously with conventional hair protecting agents.

permanent wave composition

Another aspect of the present invention provides a permanent waving composition comprising a small molecule derived from silkworm hemolymph and castor oil. In addition, the composition may further include a first agent as a reducing agent and/or a second agent as an oxidizing agent. Here, the small molecules derived from silkworm hemolymph and castor oil are the same as those described above.

At this time, based on the total weight of the permanent wave composition, the silkworm hemolymph-derived low molecule may be contained in about 0.1% by weight to about 20% by weight. Specifically, it may be about 0.1% to about 1% by weight or about 1% to about 20% by weight. Preferably, it may contain about 0.1% by weight to about 10% by weight.

In addition, castor oil may be contained in an amount of about 0.1% to about 20% by weight based on the total weight of the permanent wave composition. Specifically, it may be about 0.1% to about 1% by weight or about 1% to about 20% by weight.

The permanent wave composition may be a mixture of low molecular weight or castor oil derived from silkworm hemolymph. In addition, the permanent wave composition may be a mixture of low molecular weight derived from silkworm hemolymph and castor oil. At this time, the weight ratio of the low molecule derived from silkworm hemolymph and castor oil is about 1:10 to about 10:1, about 1:9 to about 9:1, about 1:8 to about 8:1, about 1:7 to about 7 :1, about 1:6 to about 6:1, about 1:5 to about 5:1, about 1:4 to about 4:1, about 1:3 to about 3:1, about 1:2 to about 2 :1, or about a 1:1 weight ratio. preferably about 2.5:1.

As used herein, the term "reducing agent" refers to a substance having a high property of reducing other substances while being oxidized itself. The "first agent" of the present invention is a permanent wave composition containing a reducing agent as a main component, wherein the reducing agent contained in the first agent is cystine [HO ₂ CC (NH ₂ ) HCH ₂ S-SCH ₂ C (NH ₂ ) HCO ₂ H] is reduced to cysteine [HO ₂ CC(NH ₂ )HCH ₂ SH] by cleavage of the disulfide bond to make it possible to modify the appearance of hair. As the reducing agent, thioglycolic acid or its salts (organic salts such as ammonium thioglycolate and ethanolamine thioglycolate or alkali short salts such as sodium salt), thioglycolic acid monoglycerol ester, thiolactic acid or its Salts, or esters thereof, cysteine and cysteine-hydrochloride, cysteine, N-acetyl-L-cysteine, thioacetic acid, and salts and esters thereof, or one or a mixture of two or more thereof may be used. However, it is not particularly limited as long as it is a reducing agent that can cleave disulfide bonds in hair. The amount of the reducing agent may include about 0.1% by weight to about 20% by weight based on the total weight of the permanent wave composition. Specifically, it may be about 0.1% to about 1% by weight or about 1% to about 20% by weight. It may preferably be about 1% by weight to about 10% by weight.

The first agent may be a mixture of low molecular weight derived from silkworm hemolymph or castor oil. In addition, the first agent may be a mixture of a small molecule derived from silkworm hemolymph and castor oil. At this time, the weight ratio of the low molecule derived from silkworm hemolymph and castor oil is about 1:10 to about 10:1, about 1:9 to about 9:1, about 1:8 to about 8:1, about 1:7 to about 7 :1, about 1:6 to about 6:1, about 1:5 to about 5:1, about 1:4 to about 4:1, about 1:3 to about 3:1, about 1:2 to about 2 :1, or about a 1:1 weight ratio. preferably about 2.5:1.

As used herein, the term “oxidizing agent” refers to a substance that oxidizes the other while reducing itself, contrary to the reducing agent. The "second agent" of the present invention is a permanent wave composition containing an oxidizing agent as a main component, and by applying the second agent, the oxidizing agent, the cysteine bond of the hair cut off by the reducing agent is re-formed to fix the hair in a wave shape. The oxidizing agent may be salicylic acid, sorbic acid, hydrogen peroxide, sodium bromate (NaBrO ₃ ), perchlorate, polythionate, etc., but is not particularly limited as long as it can re-form cysteine bonds of hair proteins. The content of the oxidizing agent may include about 0.5% to about 20% by weight based on the total weight of the second agent. Specifically, it may be about 0.5 wt% to about 5 wt% or about 5 wt% to about 20 wt%. It may preferably be about 5% by weight to about 15% by weight.

When the permanent wave composition of the present invention is composed of the first agent and the second agent, the low molecular weight derived from silkworm hemolymph and castor oil may be included in the first agent, the second agent, or both the first agent and the second agent.

When the low molecule derived from silkworm hemolymph and castor oil are included in the first agent, they may be included in about 5% to about 20% by weight of the total composition of the first agent. When the low molecule derived from silkworm hemolymph and castor oil are included in the second agent, they may be included in about 5% to about 20% by weight of the total composition of the second agent.

In addition, the first agent may further include additives commonly used in external preparations for hair within a range that does not impair the effects of the present invention. For example, it may include a base, a hair protectant, an alkali agent, a chelating agent, a surfactant, a pH adjusting agent, a moisturizer, a thickening agent, an antiseptic, an antioxidant, a bactericide, an anti-inflammatory agent, an antimicrobial agent, a solvent, a flavoring agent, a colorant, and the like. .

Bases are propylene glycol, cetearyl alcohol, polyoxyethylene oleyl ether, polyoxyethylene cetyl ether, stearamide MEA, oleic acid, oleyl alcohol, dimethicone, behenyl alcohol, glyceryl stearate rate SE, etc., but is not limited thereto. In this case, the base may be included in about 1% by weight to about 10% by weight based on the total amount of the first pharmaceutical composition.

Hair protectants can be used for the purpose of helping the conditioning effect and preventing damage to the hair. Sodium methyl stearoyl taurate, hydrolyzed keratin, tocopheryl acetate, cetrimonium chloride, glyceryl stearate SE, PEG-11 methyl ether dimethicone, cyclomethicone, ammonium glycyrrhizate, It may be glyceryl linoleate, linoleic acid, ceramide, mineral oil, polyquaternium-10, camellia oil, etc., but is not limited thereto.

The alkali agent helps the action of the reducing agent and is a component that adjusts the strength of the wave, and may be ammonia, ammonium carbonate, ammonium bicarbonate, sodium hydroxide, potassium hydroxide, sodium carbonate, monoethanolamine, propphenolamine, triethanolamine, etc., but is not limited thereto. don't

The chelating agent may be added for stability of thioglycolic acid, cysteine, or hydrogen peroxide, and may be ethylenediaminetetraacetic acid (EDTA) disodium, ethylenediaminetetraacetic acid tetrasodium, etc., but is not limited thereto.

Surfactants may be added to the composition to facilitate penetration of the permanent liquid into the hair, and include anionic surfactants, cationic surfactants, nonionic surfactants, and amphoteric surfactants. Anionic surfactants include ammonium lauryl sulfosuccinate, ammonium lauryl sulfate, sodium cocoyl isethionate, sodium lauryl isethionate, sodium lauryl sulfate, triethanolamine lauryl sulfate, sodium lauryl ether sulfate ( 1-3 ethylene oxide) and the like, but is not limited thereto. The nonionic surfactant may be polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene, polysorbate 80, hydrogenated castor oil derivative, fatty acid diethanolamide, glyceryl stearate, etc., but is not limited thereto. The cationic surfactant may be a tertiary aliphatic amine salt, alkyltrimethylammonium chloride, dialkyldimethylammonium chloride, and the like, but is not limited thereto. Amphoteric surfactants may be betaine, amide betaine type, sulfobetaine type, steartrimonium chloride, etc., but are not limited thereto.

Any of organic acids such as citric acid, succinic acid, tartaric acid, lactic acid, malic acid, fumaric acid, maleic acid, and glycolic acid, and inorganic acids such as hydrochloric acid, sulfuric acid, phosphoric acid, and nitric acid may be used as the pH adjuster, and sodium salts and potassium salts of these acids may be used. Salts or ammonium salts can be used in combination, and triethanolamine, sodium hydroxide, potassium hydroxide, and the like can also be used.

The humectant may be glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, sorbitol, or the like.

The thickener is specifically cetearyl alcohol, stearic acid, methylcellulose, hydroxyethylcellulose, hydroxymethylcellulose, carrageenan, carboxymethylcellulose, carboxymethylhydroxyguanine, polyquaternium-7, polyquaternium-10, etc. It may be a water-soluble polymer compound of, but is not limited thereto.

The preservative may be benzoic acid, paraoxybenzoic acid ester, a mixture of methylchloroisothiazolinone, phenoxyethanol, DMDM hydantoin, and the like.

Antioxidants may be dibutylhydroxytoluene, ascorbic acid and the like.

The disinfectant may be chlorhexidine gluconate, quaternary ammonium salt, pyroctonolamine, zinc pyrithione suspension, iodopropynylbutyl carbamate, salicylic acid, etc., but is not limited thereto.

The anti-inflammatory component may be monoammonium glycyrrhizinate, dipotassium glycyrrhizinate, stearyl glycyrrhizinate, chamomile, alpha bisabolol, allantoin, and mixtures thereof.

Antimicrobial components are specifically phenoxyethanol, chlorhexidine, chlorhexidine gluconate, piroctonolamine, ketoconazole, arnica extract, iodopropynylbutyl carbamate, benzalkonium chloride, benzentonium chloride, benzoic acid and its salts, benzyl alcohol, lavender, rosemary, salicylic acid, triclocarban, zinc pyrithione suspension, and mixtures thereof, but are not limited thereto.

The solvent may be purified water, ethanol, Tween 20, cyclomethicone, mineral oil, dimethicone, or the like.

The solubilizing agent may be isopropyl myristate, polyethylene glycol, medium chain fatty acid triglyceride, hydrocarbons, glycols, etc., but is not limited thereto.

Components used in general formulations for scalp and hair may be used as the flavoring agent and coloring agent.

The treatment method of the cosmetic composition according to the present invention is not particularly limited as long as it exhibits the effects of the present invention. Alternatively, it may be treated by a method such as directly applying or spraying to the hair or scalp, but is not limited thereto.

Another aspect of the present invention provides a permanent wave kit including the cosmetic composition for protecting and restoring hair, a first agent as a reducing agent, and a second agent as an oxidizing agent. At this time, the cosmetic composition for hair protection and restoration, reducing agent, first agent, oxidizing agent, second agent, and permanent agent are the same as described above.

The method of using the permanent wave kit of the present invention is not particularly limited in showing the effect of the present invention, but the cosmetic composition for hair protection and restoration is used before application of the reducing agent as the first agent, immediately before forming waves after application, and permanent wave treatment It can be used by applying it to the hair during the process. Preferably, the first agent may be applied before application and immediately before forming a wave after application.

Beauty method to improve permanent efficiency and hair damage

Another aspect of the present invention comprises the steps of treating hair with low molecules derived from silkworm hemolymph and castor oil; And it provides a cosmetic method for improving the permanent efficiency and hair damage, including the step of performing a permanent on the hair.

At this time, the silkworm hemolymph-derived small molecule, castor oil, permanent, and hair damage are as described above.

At this time, the low molecule derived from silkworm hemolymph and castor oil may be mixed and treated simultaneously or sequentially. When the silkworm hemolymph-derived small molecule and castor oil are mixed and treated, the ratio of the silkworm hemolymph-derived small molecule and castor oil is about 1:10 to about 10:1, about 1:9 to about 9:1, about 1:8 to About 8:1, about 1:7 to about 7:1, about 1:6 to about 6:1, about 1:5 to about 5:1, about 1:4 to about 4:1, about 1:3 to about They may be mixed in a weight ratio of about 3:1, about 1:2 to about 2:1, or about 1:1 . Preferably, they may be mixed in a weight ratio of about 2.5:1.

In addition, when the silkworm hemolymph-derived small molecule and castor oil are sequentially treated, the castor oil can be treated after the silkworm hemolymph-derived small molecule treatment, and the silkworm hemolymph-derived small molecule can be treated after the castor oil treatment. Preferably, castor oil can be treated after treatment with silkworm hemolymph-derived small molecules. At this time, the treatment ratio of the silkworm hemolymph-derived small molecule and castor oil to the hair is about 1:10 to about 10:1, about 1:9 to about 9:1, about 1:8 to about 8:1, about 1: 7 to about 7:1, about 1:6 to about 6:1, about 1:5 to about 5:1, about 1:4 to about 4:1, about 1:3 to about 3:1, about 1: 2 to about 2:1, or about 1:1 by weight. A weight ratio of about 2.5:1 may be preferred.

As used herein, the term "improvement" refers to any action that reduces or beneficially changes hair damage caused by permanent treatment by treatment with the composition.

Another aspect of the present invention provides a use of a small molecule derived from silkworm hemolymph for hair protection and restoration. The silkworm hemolymph-derived small molecule is the same as described above. At this time, castor oil can be used together with silkworm hemolymph-derived small molecules for mimetic protection and restoration purposes. Here, protection, restoration and castor oil are the same as described above.

Another aspect of the present invention provides the use of a cosmetic preparation for hair protection and restoration comprising a small molecule derived from silkworm hemolymph. In this case, the formulation may further include castor oil, and the low molecular weight derived from silkworm hemolymph, protection, restoration, and castor oil are the same as described above.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

I. Sample preparation

In order to confirm the hair protection effect of iron permanent treatment of low molecular weight derived from silkworm hemolymph and castor oil pretreatment according to hair damage, virgin hair, bleach 1 time hair, bleach 2 times ) hair and bleach 3 times hair were used. The treated groups were divided into 4 groups, and were treated with untreated (group A), castor oil (group B), small molecules derived from silkworm hemolymph (group C), and castor oil (group D) after treatment with small molecules derived from silkworm hemolymph. And after the permanent treatment, observation of hair epidermal shape through scanning electron microscopy (SEM), energy dispersive X-ray spectrometer, EDS) was used to confirm sulfur (S) content, tensile strength, elongation, moisturizing power, and wave efficiency (FIG. 5).

Example 1. Production of damaged hair

Experimental hair was purchased from China's dark brown, 20 to 25 cm long, chemical-free raw hair from a Morris wig seller. 160 bundles were made by bonding the cut parts with silicone, each weighing 1.2 g, and 40 bundles per group were separated to form a total of 4 hair groups: natural hair, bleached hair once, bleached hair twice, and bleached hair three times. prepared.

For bleaching, mix the first agent, a mixture of potassium sulfate and ammonium sulfate, and the second agent, 20 hydrogen peroxide (volume 6%) in a ratio of 1:1.3, apply the chemical to the hair, and then wrap the hair with aluminum foil to block external air. It was carried out in a way.

Bleaching 1 time Hair was prepared by leaving it naturally for 20 minutes after bleaching 1 time and washing with neutral shampoo. Bleaching 2 times The hair was bleached once, then additionally bleached, left for 20 minutes naturally, and then washed with neutral shampoo. Bleaching 3 times The hair was bleached 2 times, then additionally bleached once, left for 20 minutes naturally, and then washed with neutral shampoo.

Example 2. Production of small molecules derived from silkworm hemolymph and castor oil

Silkworm hemolymph was provided with silkworm hemolymph that had been exempted from deliberation by the Bioethics Committee of Cha University of Science and Technology (Task management number: 1044305-202012-BR-056-01), and among silkworm hemolymph, silkworm hemolymph with a low molecular weight of 10 KDa or less was found. Experiments were performed using lymph.

Specifically, silkworm hemolymph-derived low-molecular silkworm hemolymph of 10 KDa or less was used among silkworm hemolymph, and was prepared through the following method.

First, 1 kg of silkworm was treated with liquid nitrogen (-196 ° C, 70 hours), and then freeze-dried to prepare dried silkworms. Silk glands were removed from the dried silkworms using a ball mill with a diameter of 1 cm to 5 cm, and hemolymph was separated. After pulverizing the separated hemolymph, 3% of water based on the weight of the pulverized material was added and stirred at 200 rpm for 30 minutes to prepare a hemolymph lysate. The hemolymph lysate was filtered through a 200 μm to 1,000 μm sieve and heat-treated at 60° C. for 30 minutes to inactivate dopaquinone, a toxic substance. Thereafter, the supernatant was recovered by centrifugation at 13,000 rpm for 20 minutes, and the low molecular weight material derived from silkworm hemolymph was filtered and separated using a 10 KDa ultrafiltration membrane. A schematic diagram of the manufacturing process of the silkworm hemolymph-derived small molecule is shown in FIG. 3. The molecular weight of the isolated silkworm hemolymph-derived small molecule was analyzed by liquid chromatography mass spectrometry. As a result, the size of the small molecule material derived from silkworm hemolymph was measured to be 258 Da to 589 Da (FIG. 3).

In addition, castor oil was purchased and used as a commercially available product (Now food, India) and 100% natural castor oil extracted primarily by cold pressing.

Example 3. Iron permanent treatment

After neutral shampooing, healthy hair and bleached hair were classified into groups A, B, C, and D, respectively, and softened by applying thioglycolic acid. Natural hair was left at room temperature for 25 minutes, bleached hair for 10 minutes at room temperature, hair with two bleaches for 7 minutes at room temperature, and hair with three bleaches for 5 minutes at room temperature, and then shampooed with lukewarm water. Prior to treatment of the experimental samples, the moisture content was determined. At this time, the natural hair showed a moisture content of 25%, hair bleached 1 time 20%, hair bleached 2 times 15%, and hair bleached 3 times 10%. In addition, 4 types of hair were pretreated with castor oil (group D) after untreated (control group, group A), castor oil (group B), low molecule derived from silkworm hemolymph (group C), or low molecular weight derived from silkworm hemolymph. . Specifically, the pretreatment was carried out by applying 1 mg of silkworm hemolymph-derived low molecule per bundle of hair before and after softening treatment, and then sequentially applying 0.4 mg of castor oil to the hair after softening treatment.

The iron permanent device was a product of Yuhan Beauty Development Co. (Ezekiel Iron), and a circular device with a diameter of 13 mm, temperature control, and a cover was used (FIG. 5d). The treatment temperature was 140 ° C for natural hair, 130 ° C for 1 bleaching hair, 120 ° C for 2 bleaching hair, and 110 ° C for 3 bleaching hair, respectively.

After the treatment, the hair was naturally left for 10 minutes to cool the residual heat of the hair, and then treated with an oxidizing agent. Oxidizing agent treatment was carried out for a total of 8 minutes by spraying twice for 4 minutes each. After the oxidizing agent treatment was completed, the hair was shampooed and naturally dried (FIG. 6).

II. Confirmation of hair protection effect of castor oil and low molecular weight derived from silkworm hemolymph

Example 4. Observation of hair epidermis using scanning electron microscopy

In order to observe the morphological change of the hair epidermis according to the pretreatment material during the iron permanent treatment, the sample hair was cut to a length of 3 cm and fixed to a copper tape. Sample hair was plated with platinum for 1 minute and 50 seconds to a thickness of 20 nm, and images were taken with a field emission scanning microscope (JSM-6700F, JOEL). The images were magnified at 800X and 1,000X magnification under the condition of 15 kV, and the morphological changes of the hair epidermis were confirmed through the photographed images. As a result, epidermal cuticles were tidied up by the action of the hot air balloon used during the iron permanent treatment, and epidermal adhesion was observed, but morphological changes in epidermal damage were observed depending on the number of times of hair bleaching and pretreatment conditions (FIGS. 8 to 11). ).

In the case of the control group without pretreatment, lifting of the epidermis was the most common, and epidermal tearing and exfoliation were observed in the hair after bleaching twice and bleaching hair three times (FIG. 8).

In the case of the silkworm hemolymph-derived small molecule treatment group, the epidermal adhesion was observed to be rather low in the hair of three bleaching times. This is because the silkworm hemolymph-derived small molecule was mainly absorbed into the cortex of the hair and had little effect on the image taken of the epidermal state of the hair (FIG. 9). In the case of the castor oil treatment group, epidermal adhesion was generally observed. Through the above results, when castor oil was treated, castor oil adhered to the cell membrane complex and acted as an oil film coating, and this effect showed a difference according to the number of decolorization (FIG. 10).

In the case of the small molecule derived from silkworm hemolymph and the castor oil-treated group, a scale form, which is the form showing the highest epidermal adhesion, was observed. The above result is a result of the interaction between hair outer skin protection and hair inner protection reflected internally and externally as the silkworm hemolymph-derived low-molecular protein is absorbed into the cortex of the hair and castor oil acts as an oil film coating on the epidermis of the hair (FIG. 11 ).

Example 5. Surface Elemental Sulfur (S) Content Analysis Using Energy Dispersive X-ray Spectrometry

In order to analyze the cysteine sulfur (S) content of hair, the sulfur component was measured through energy dispersive X-ray spectroscopy (EDS, FIG. 7a) using a field emission scanning microscope (JSM-6700F, JEOL). The measurement magnification was analyzed at 3,000X. At this time, all statistical analysis was performed using Sigma Plot 12.0 Software (Systat Software, San Jose, CA). Significant differences between treatment groups were evaluated by one-way ANOVA followed by post-correction for multiple comparisons. Quantitative results were presented using mean standard deviation, and statistical significance was defined as p<0.05.

Example 5.1. Sulfur content descriptive statistical analysis according to pretreatment

Using energy dispersive X-ray spectroscopy (EDS), the elemental sulfur component of the surface of the hair according to the pretreatment was measured (FIG. 12), and descriptive statistical analysis was performed on the sulfur content (Table 1).

treatment group number minimum max value average Standard Deviation untreated natural hair 10 2.83 4.19 3.7170 0.36344 Bleaching 1 time 10 3.19 3.95 3.4890 0.26215 bleach 2 times 10 2.37 3.44 3.1670 0.33009 bleach 3 times 10 1.73 2.39 2.1050 0.24103 castor oil natural hair 10 2.92 3.87 3.5380 0.32245 Bleaching 1 time 10 2.92 3.87 3.5320 0.32214 bleach 2 times 10 3.17 3.96 3.4710 0.24420 bleach 3 times 10 2.04 3.24 2.8040 0.38193 silkworm hemolymph
small molecule natural hair 10 3.67 3.89 3.7750 0.07605 Bleaching 1 time 10 3.04 3.74 3.4590 0.21079 bleach 2 times 10 3.08 3.81 3.2310 0.21434 bleach 3 times 10 2.72 3.24 3.0200 0.17250 silkworm hemolymph
small molecules and
castor oil natural hair 10 3.70 4.27 4.0320 0.16772 Bleaching 1 time 10 3.21 3.77 3.5260 0.20321 bleach 2 times 10 3.03 4.03 3.4910 0.27339 bleach 3 times 10 3.04 3.63 3.3140 0.16554

In addition, as a result of comparing the sulfur content by natural hair and hair damage condition in each treatment group, the sulfur content was the highest in the natural hair in the untreated group, and the sulfur content decreased as the hair was damaged due to bleaching. In the castor oil treatment group, the sulfur content was the lowest in the bleached hair 3 times, and the sulfur content was similar in the other treatment groups. The group treated with silkworm hemolymph-derived small molecules also showed the lowest sulfur content in hair after 3 bleaching cycles. In addition, in the group treated with silkworm hemolymph and castor oil, the hair with three bleaching times showed the lowest sulfur content.

However, when the sulfur content of each treatment group was compared within the same hair damage condition, the sulfur content of the silkworm hemolymph-derived low molecular weight and castor oil treatment groups was the highest in all hair damage conditions.

Example 5.2. Analysis of variance to measure sulfur content in hair according to pretreatment

Example 5.2.1. Sulfur content measurement and analysis according to pretreatment of natural hair

The sulfur (S) content of natural hair according to untreated, castor oil, silkworm hemolymph-derived small molecule, or silkworm hemolymph-derived small molecule and castor oil treatment was measured, and one-way analysis of variance (Table 2) and post hoc test were performed. was performed (Table 3).

Sulfur (S) content sum of squares df mean square F significance probability between treatment groups 1.252 3 0.417 6.184 ^** 0.002 treatment group-within 2.430 36 0.067 Sum 3.682 39

*p<0.01

treatment group number Subgroup for significance level = 0.05 One 2 Post test results castor oil 10 3.5380 Castor oil and small molecules derived from silkworm hemolymph > Small molecules derived from silkworm hemolymph > Untreated > Castor oil untreated 10 3.7170 3.7170 Small molecule derived from silkworm hemolymph 10 3.7750 3.7750 castor oil and
Small molecule derived from silkworm hemolymph 10 4.0320 significance probability 0.262 0.079

As a result of analyzing the sulfur content according to each treatment of natural hair, the F value between the treatment groups was 6.184, and the significance probability was 0.002, showing a significant difference at the p<0.01 level (Table 2). In the post-hoc test results, castor oil and silkworm hemolymph-derived low-molecular treatment group appeared the highest, followed by silkworm hemolymph-derived small molecule, untreated, and castor oil-treated groups (Table 3).

Example 5.2.2. Sulfur content measurement and analysis according to pretreatment of hair after bleaching once

Sulfur (S) content of untreated, castor oil, silkworm hemolymph-derived small molecule, or silkworm hemolymph-derived small molecule and castor oil treatment after bleaching hair was measured, and one-way analysis of variance was performed on this (Table 4 ).

Sulfur (S) content sum of squares df mean square F significance probability between treatment groups 0.035 3 0.012 0.180 0.909 treatment group-within 2.324 36 0.065 Sum 2.359 39

As a result of analyzing the sulfur content according to each treatment in the bleached hair once, the F value between the treatment groups was 0.180, and the significance probability was 0.909, showing no significant difference.

Example 5.2.3. Sulfur content measurement and analysis according to pre-treatment of hair after bleaching twice

Sulfur (S) content in hair untreated, castor oil, low molecular weight derived from silkworm hemolymph or low molecular weight derived from silkworm hemolymph and castor oil treatment was measured, and one-way analysis of variance (Table 5) and post hoc test was carried out (Table 6).

Sulfur (S) content sum of squares df mean square F significance probability between treatment groups 0.818 3 0.273 3.769 ^* 0.019 treatment group-within 2.603 36 0.072 Sum 3.421 39

*p<0.05

treatment group number Subgroup for significance level = 0.05 One Post test results untreated 10 3.1670 Castor oil and silkworm hemolymph-derived small molecules = silkworm hemolymph-derived small molecules = castor oil = untreated castor oil 10 3.2310 Small molecule derived from silkworm hemolymph 10 3.4710 castor oil and
Small molecule derived from silkworm hemolymph 10 3.4910 significance probability 0.082

As a result of analyzing the sulfur content according to each treatment of the bleached hair twice, the F value between the treatment groups was 3.769 and the significance probability was 0.019, showing a significant difference at the p <0.05 level (Table 5). In the post-hoc test results, castor oil and silkworm hemolymph-derived small molecules treatment group showed the highest, followed by silkworm hemolymph-derived small molecule, castor oil, and untreated treatment group (Table 6).

Example 5.2.4. Sulfur content measurement and analysis according to pre-treatment of hair bleached 3 times

Sulfur (S) content of hair was measured for three times of bleaching according to untreated, castor oil, silkworm hemolymph-derived low-molecular or silkworm hemolymph-derived low-molecular and castor oil treatment, and one-way analysis of variance (Table 7, *** p<0.001) and post hoc tests were performed (Table 8).

Sulfur (S) content sum of squares df mean square F significance probability between treatment groups 7.952 3 2.651 40.602 ^*** 0.000 treatment group-within 2.350 36 0.065 Sum 10.302 39

***p<0.001

treatment group number Subgroup for significance level = 0.05 One 2 3 Post test results untreated 10 2.1050 Castor Oil and Silkworm Hemolymph-derived Small Molecules > Silkworm Hemolymph-derived Small Molecules > Castor Oil > Untreated castor oil 10 2.8040 Small molecule derived from silkworm hemolymph 10 3.0200 3.0200 castor oil and
Small molecule derived from silkworm hemolymph 10 3.3140 significance probability 1.000 0.327 0.104

As a result of analyzing the sulfur content according to each treatment of the bleached hair three times, the F value between the treatment groups was 40.602, and the significance probability was 0.000, showing a significant difference at the p <0.001 level (Table 7). In the post-hoc test results, castor oil and silkworm hemolymph-derived small molecules treatment group showed the highest, followed by silkworm hemolymph-derived small molecule, castor oil, and untreated treatment group (Table 8).

Example 6. Hair tensile strength and elongation analysis according to pretreatment

Example 6.1. Analysis of tensile strength of hair according to pretreatment

Tensile strength is a force representing the strength of a material, and refers to the value obtained by dividing the maximum load that a material can withstand when pulled to be cut by the cross-sectional area of the material. In order to measure the tensile strength of the hair according to the pretreatment, the sample hair was fixed in a universal testing system (universal testing systems up + 50KN, INSTROM, FIG. 7b) at a gauge distance of 20 mm, and 10 times at a speed of 10 mm / min. measured.

As a result, the tensile strength of natural hair in the untreated group was 256.98 (MPa) for natural hair, 210.89 (MPa) for hair with 1 bleaching, 163.45 (MPa) for hair with 2 bleaching, and 160.10 (MPa) with 3 bleaching, compared to damaged hair. appeared high.

In the castor oil treatment group, the tensile strength was high in damaged hair with natural hair 3842.43 (MPa), bleached hair 7014.23 (MPa), bleached hair twice 5532.32 (MPa), and hair bleached 3 times 14865.70 (MPa). .

In the silkworm hemolymph-derived small molecule treatment group, natural hair was 3959.06 (MPa), bleached hair 3941.01 (MPa), bleached hair twice 3543.15 (MPa), and bleached hair 3 times 3314.13 (MPa), showing high tensile strength in damaged hair. appear.

In the group treated with castor oil and small molecules derived from silkworm hemolymph, natural hair was 4154.80 (MPa), bleached hair 6216.42 (MPa), bleached hair twice, 5712.28 (MPa), and bleached hair 3 times 5100.09 (MPa). The intensity was high (FIG. 13).

The tensile strength was low in the damaged hair of the untreated group, but the tensile strength was high in the damaged hair using a combination of castor oil and small molecules derived from silkworm hemolymph.

These results are the result of the amino acid component of silkworm hemolymph replenishing the lost hair cortex interstitial substances, and castor oil adheres to the cell membrane complex to coat the hair epidermis with an oil film to protect the hair.

Example 6.2. Analysis of hair elongation according to pretreatment

Elongation refers to the maximum ratio of elongation without breaking when weight is applied, and is expressed as a percentage by dividing the difference between the maximum elongated length and the original length by the original length. In order to analyze the elongation of the hair according to the pretreatment, it was measured under the same conditions as in Example 6.1 using a universal testing system (up + 50KN, INSTROM) (Table 9).

breaking load
(Load at Break)
(kgf) breaking stress
(Stress at Break)
(MPa) maximum load
(Maximum Load)
(kgf) maximum load strain
(Strain at Maximum Load)
(%) maximum load stress
(Stress at Maximum Load)
(MPa) Young's modulus
(Automatic Young's modulus)
(MPa) breaking point
(cut point) untreated natural hair -0.02 -71.20 0.07 88.36 256.98 3838.94 center Bleaching 1 time -0.04 -99.94 0.06 72.48 210.89 2999.74 center bleach 2 times -0.03 -150.96 0.05 99.97 163.45 2960.60 center bleach 3 times -0.02 -69.13 0.05 134.06 160.10 2536.22 center castor oil natural hair -0.03 -91.46 0.05 76.64 93.73 3842.43 center Bleaching 1 time -0.03 -87.88 0.04 77.01 128.53 7014.23 center bleach 2 times -0.03 -107.01 0.05 99.54 179.22 5532.32 center bleach 3 times -0.01 -48.78 0.03 134.23 183.58 4865.70 center Small molecule derived from silkworm hemolymph natural hair -0.03 -116.81 0.05 73.53 164.65 3959.06 center Bleaching 1 time -0.04 -134.86 0.06 82.34 204.82 3941.01 center bleach 2 times -0.05 -167.93 0.07 108.09 240.09 3543.15 center bleach 3 times -0.03 -107.22 0.03 113.42 262.32 3314.33 center castor oil and
Small molecule derived from silkworm hemolymph natural hair -0.04 -152.02 0.05 83.78 164.44 4154.80 center Bleaching 1 time -0.03 -113.18 0.05 83.31 169.27 6216.42 center bleach 2 times -0.02 -54.14 0.08 105.12 263.08 5712.18 center bleach 3 times -0.01 -20.08 0.09 156.00 328.69 5100.09 center

The reason why the elongation rate of hair is increased is because the oil-moisture balance is established to maintain healthy hair, or when the cysteine bonds that have been swollen after softening are weakened and loosened, the elongation force becomes stronger and the length of breakage becomes longer. On the other hand, when the hair becomes dry, the hair protein hardens (cured state), and when the amount of oil and moisture is insufficient, the hair splits and breaks.

Looking at the above results, the higher the number of times of discoloration in all four groups, the higher the elongation value.

Looking at the elongation by treatment group, the untreated group showed the highest value at 88.36% in natural hair, and the group treated with low molecular weight derived from castor oil and silkworm hemolymph showed the highest value at 83.31% in hair bleached once. In addition, in the hair bleached 2 times, the silkworm hemolymph-derived small molecule treatment group showed the highest rate at 108.09%, and in the hair bleached 3 times, the castor oil and silkworm hemolymph-derived small molecule treatment group showed the highest rate at 156%.

The increase in elongation in the damaged hair of the 4 groups is the result of the weakening of the binding force of the hair tissue loosened during the bleaching process, and the high elongation in the silkworm hemolymph derived small molecule treatment group is absorbed into the cortex of the hair. This is the result of increased elongation due to the binding of the substance to the hair tissue.

Example 7. Analysis of hair moisturizing power measurement according to pretreatment

In order to measure the moisturizing power of the hair after the iron permanent treatment, 0.65 g of each test sample hair was immersed in distilled water for 30 minutes. The weight was measured after pressing and dehydrating the hair on a water removal absorption filter paper. After that, the hair was dried in a dryer (ThermoStable SOF-105, DEIHAN, Fig. 7c) at 35 °C for 20 minutes. After drying, the weight of the hair was measured to compare the moisturizing power before and after drying. At this time, all statistical analysis was performed using Sigma Plot 12.0 Software (Systat Software, San Jose, CA). Significant differences between treatment groups were evaluated by one-way ANOVA followed by post-correction for multiple comparisons. Quantitative results were presented using mean standard deviation, and statistical significance was defined as p<0.05.

Example 7.1. Descriptive statistical analysis of hair moisturizing power according to pretreatment

Moisturizing power of hair according to control, untreated, small molecule derived from silkworm hemolymph, castor oil, low molecular weight derived from silkworm hemolymph, and castor oil treatment was measured, and descriptive statistical analysis was performed (Table 10).

moisturizing power number minimum max value average Standard Deviation untreated natural hair water immersion 10 1.12 1.45 1.2390 0.11609 dry 10 0.66 0.88 0.7790 0.07781 Bleaching 1 time water immersion 10 1.11 1.34 1.2320 0.06408 dry 10 0.63 0.90 0.7670 0.08845 bleach 2 times water immersion 10 1.11 1.60 1.2710 0.14154 dry 10 0.69 1.07 0.7980 0.11574 bleach 3 times water immersion 10 1.15 1.39 1.2560 0.08066 dry 10 0.61 1.01 0.7740 0.11530 castor oil natural hair water immersion 10 1.12 1.70 1.2820 0.16877 dry 10 0.71 1.16 0.8310 0.12333 Bleaching 1 time water immersion 10 1.05 1.28 1.1760 0.08127 dry 10 0.37 0.88 0.7190 0.14866 bleach 2 times water immersion 10 1.15 1.35 1.2520 0.06426 dry 10 0.62 1.09 0.7980 0.12726 bleach 3 times water immersion 10 1.11 1.47 1.2390 0.11210 dry 10 0.65 0.93 0.7810 0.09515 Small molecule derived from silkworm hemolymph natural hair water immersion 10 1.07 1.29 1.1810 0.06967 dry 10 0.64 0.89 0.7620 0.07843 Bleaching 1 time water immersion 10 1.07 1.41 1.2560 0.10233 dry 10 0.65 1.16 0.8280 0.14520 bleach 2 times water immersion 10 1.09 1.32 1.2200 0.07409 dry 10 0.61 0.91 0.7650 0.09204 bleach 3 times water immersion 10 1.09 1.35 1.2320 0.07729 dry 10 0.71 0.93 0.7850 0.06948 castor oil and
Small molecule derived from silkworm hemolymph natural hair water immersion 10 1.03 1.36 1.1780 0.10401 dry 10 0.65 0.97 0.7900 0.10873 Bleaching 1 time water immersion 10 1.02 1.60 1.2130 0.16780 dry 10 0.62 104 0.8060 0.16780 bleach 2 times water immersion 10 1.09 1.50 1.2560 0.11088 dry 10 0.71 1.18 0.8440 0.13558 bleach 3 times water immersion 10 1.06 1.30 1.2140 0.10348 dry 10 0.62 1.07 0.7900 0.3433

In the case of natural hair, the castor oil treatment group showed the highest moisturizing power, and in the case of hair bleached once, the moisturizing power of the small molecule treatment group derived from silkworm hemolymph was highest. In addition, in the case of 2 times bleaching hair and 3 times bleaching hair, the moisturizing power was the highest in the low molecular weight derived from silkworm hemolymph and castor oil treatment groups. Through the above results, it was confirmed that the moisturizing power appeared high according to the use of the protective material in the damaged hair.

Example 7.2. Hair moisturizing power measurement and analysis according to pretreatment

Example 7.2.1. Moisturizing power after pre-treatment of natural hair and after drying treatment Measurement of moisturizing power after untreated natural hair, castor oil, small molecules derived from silkworm hemolymph, or low molecular weight derived from silkworm hemolymph and castor oil treatment, and batched accordingly Analysis of variance was performed (Table 11).

natural hair sum of squares df mean square F significance probability water immersion between treatment groups 0.075 3 0.025 1.733 0.178 treatment group-within 0.519 36 0.014 Sum 0.594 39 dry between treatment groups 0.026 3 0.009 0.878 0.461 treatment group-within 0.353 36 0.010 Sum 0.379 39

In natural hair, looking at the results of moisture measurement analysis when supplying moisture (water immersion) in each treatment group, the F value between treatment groups was 1.733 and the significance probability was 0.178, showing no significant difference. In addition, in the results of the analysis of the moisturizing power when drying the hair of each treatment group, the F value between the treatment groups was 0.878 and the significance probability was 0.461, showing no significant difference.

Example 7.2.2. Moisturizing power measurement and analysis during moisture and drying treatment according to pre-treatment of hair after bleaching once

Moisturizing power was measured according to untreated, castor oil, silkworm hemolymph-derived small molecules, or silkworm hemolymph-derived small molecules and castor oil treatment of hair once bleached, and one-way analysis of variance was performed (Table 12).

Bleaching 1 time sum of squares df mean square F significance probability water immersion between treatment groups 0.034 3 0.011 0.925 0.439 treatment group-within 0.444 36 0.012 Sum 0.478 39 dry between treatment groups 0.069 3 0.023 1.320 0.283 treatment group-within 0.625 36 0.017 Sum 0.693 39

Looking at the results of the analysis of the moisturizing power measurement when supplying moisture (water immersion) in each treatment group in the bleached hair once, the F value between the treatment groups was 0.925 and the significance probability was 0.439, showing no significant difference. In addition, in the results of the analysis of the moisturizing power when drying the hair of each treatment group, the F value between the treatment groups was 1.320 and the significance probability was 0.283, showing no significant difference.

Example 7.2.3. Moisturizing power measurement and analysis during moisture and drying treatment according to pre-treatment of hair after bleaching twice

Moisturizing power was measured according to untreated, castor oil, silkworm hemolymph-derived low-molecule, or silkworm hemolymph-derived low-molecular-weight and castor oil treatment of hair after two bleaching times, and a one-way ANOVA was performed (Table 13).

bleach 2 times sum of squares df mean square F significance probability water immersion between treatment groups 0.014 3 0.005 0.439 0.727 treatment group-within 0.377 36 0.010 Sum 0.391 39 dry between treatment groups 0.032 3 0.011 0.747 0.531 treatment group-within 0.508 36 0.014 Sum 0.540 39

Looking at the results of the analysis of the moisturizing power measurement when supplying moisture (water immersion) in each treatment group in the hair of twice bleached hair, the F value between the treatment groups was 0.439 and the significance probability was 0.727, showing no significant difference. In addition, in the results of the analysis of the moisturizing power when drying the hair of each treatment group, the F value between the treatment groups was 0.747 and the significance probability was 0.531, showing no significant difference.

Example 7.2.4. Moisturizing power measurement and analysis during moisture and drying treatment according to pre-treatment of hair after bleaching 3 times

Moisturizing power was measured according to untreated, castor oil, silkworm hemolymph-derived low-molecule, or silkworm hemolymph-derived low-molecular-weight and castor oil treatment of hair three times after bleaching, and one-way analysis of variance was performed on this (Table 14).

bleach 3 times sum of squares df mean square F significance probability water immersion between treatment groups 0.009 3 0.003 0.337 0.799 treatment group-within 0.323 36 0.009 Sum 0.332 39 dry between treatment groups 0.001 3 0.000 0.040 0.989 treatment group-within 0.407 36 0.011 Sum 0.408 39

Looking at the results of the analysis of the moisturizing power measurement when supplying moisture (water immersion) in each treatment group in the hair of three bleaching times, the F value between the treatment groups was 0.337 and the significance probability was 0.799, showing no significant difference. In addition, in the results of the analysis of the moisturizing power when drying the hair of each treatment group, the F value between the treatment groups was 0.040 and the significance probability was 0.989, showing no significant difference.

Example 8. Measurement of wave efficiency according to pretreatment

In order to measure the wave efficiency, the hair was washed with a neutral shampoo after ironing and dried. At this time, the hair was dried by hanging down in the vertical direction. The dried hair was fixed on A4 paper, and the length was measured using a digital caliper (BD-DC300P 0-300, BLUETEC, Fig. 7d). At this time, the shorter the length, the higher the wave efficiency. At this time, all statistical analysis was performed using Sigma Plot 12.0 Software (Systat Software, San Jose, CA). Significant differences between treatment groups were evaluated by one-way ANOVA followed by post-correction for multiple comparisons. Quantitative results were presented using mean standard deviation, and statistical significance was defined as p<0.05.

Example 8.1. Descriptive statistical analysis of wave efficiency according to pretreatment

The wave efficiency of hair according to control, untreated, small molecule derived from silkworm hemolymph, castor oil, or treated with small molecule derived from silkworm hemolymph and castor oil was confirmed (Table 15 and FIGS. 14 to 17).

wave efficiency number minimum max value average Standard Deviation untreated natural hair 10 12.52 13.50 13.1520 0.34835 Bleaching 1 time 10 13.47 14.80 14.1080 0.39813 bleach 2 times 10 14.79 15.12 15.0180 0.10932 bleach 3 times 10 15.29 16.20 15.7430 0.34270 castor oil natural hair 10 12.44 13.80 13.0560 0.33971 Bleaching 1 time 10 13.52 14.30 13.9160 0.26192 bleach 2 times 10 13.52 15.10 14.2570 0.37898 bleach 3 times 10 14.78 15.90 15.3250 0.42971 Small molecule derived from silkworm hemolymph natural hair 10 12.28 13.40 12.9500 0.40224 Bleaching 1 time 10 13.45 14.20 13.8730 0.25246 bleach 2 times 10 13.59 14.76 14.1660 0.30130 bleach 3 times 10 14.70 15.88 15.1540 0.47313 castor oil
and
Small molecule derived from silkworm hemolymph natural hair 10 12.45 13.20 12.9020 0.28863 Bleaching 1 time 10 13.40 14.30 13.8460 0.28250 bleach 2 times 10 13.45 14.45 14.1220 0.31580 bleach 3 times 10 14.62 15.11 14.9000 0.18439

Looking at the wave efficiency results for each hair damage condition in each treatment group, the wave efficiency of natural hair was the highest in all treatment groups, and the wave efficiency decreased as the hair damage progressed.

In addition, looking at the wave efficiency of each treatment group under each hair damage condition, the wave efficiency of the small molecule derived from silkworm hemolymph and castor oil treatment group was the highest compared to the untreated group.

Through the above results, it was confirmed that pretreatment with silkworm hemolymph-derived small molecule and castor oil increased the efficiency of the wave.

Example 8.2. Variance analysis of wave efficiency according to pretreatment

Example 8.2.1. Analysis of wave efficiency of natural hair according to pretreatment

Wave efficiency was measured according to untreated natural hair, castor oil, silkworm hemolymph-derived small molecule, or silkworm hemolymph-derived small molecule and castor oil treatment, and one-way ANOVA was performed (Table 16).

wave efficiency sum of squares df mean square F significance probability between treatment groups 0.374 3 0.125 1.036 0.388 treatment group-within 4.337 36 0.120 Sum 4.711 39

As a result of analyzing the wave efficiency according to each treatment in natural hair, the F value between treatment groups was 1.036 and the significance probability was 0.388, showing no significant difference.

Example 8.2.2. Analysis of wave efficiency of hair after bleaching once according to pretreatment

The wave efficiency was measured according to untreated, castor oil, silkworm hemolymph-derived small molecule, or silkworm hemolymph-derived small molecule and castor oil treatment of hair once bleached, and one-way ANOVA was performed (Table 17).

wave efficiency sum of squares df mean square F significance probability between treatment groups 0.421 3 0.140 1.513 0.228 treatment group-within 3.336 36 0.093 Sum 3.756 39

As a result of the wave efficiency measurement analysis according to each treatment in the hair bleached once, the F value between the treatment groups was 1.513 and the significance probability was 0.228, showing no significant difference.

Example 8.2.3. Analysis of wave efficiency of hair after bleaching twice according to pretreatment

Wave efficiency was measured according to untreated, castor oil, silkworm hemolymph-derived small molecule, or silkworm hemolymph-derived small molecule and castor oil treatment of hair twice bleached, and for this, one-way analysis of variance (Table 18) and post hoc test (Table 19) was carried out.

wave efficiency sum of squares df mean square F significance probability between treatment groups 5.341 3 1.780 20.576 ^*** 0.000 treatment group-within 3.115 36 0.087 Sum 8.455 39

***p<0.001

treatment group number Subgroup for significance level = 0.05 One 2 Post test result castor oil and
Small molecule derived from silkworm hemolymph 10 14.1220 Castor oil and small molecules derived from silkworm hemolymph > Small molecules derived from silkworm hemolymph > Castor oil > Untreated Small molecule derived from silkworm hemolymph 10 14.1660 castor oil 10 14.2570 untreated 10 15.0180 significance probability 0.789 1.000

As a result of the wave efficiency measurement analysis according to each treatment in the hair twice bleached, the F value between the treatment groups was 20.576, and the significance probability was 0.000, showing a significant difference at the p <0.05 level (Table 18). In the case of the post hoc test results, castor oil and silkworm hemolymph-derived small molecules showed the highest wave efficiencies, followed by silkworm hemolymph-derived small molecules, castor oil, and untreated treatment group (Table 19).

Example 8.2.4. Wave efficiency analysis of hair bleached 3 times according to pretreatment

The wave efficiency was measured according to untreated, castor oil, silkworm hemolymph-derived small molecule, or silkworm hemolymph-derived small molecule and castor oil treatment of the hair three times after bleaching, and a one-way ANOVA was performed (Table 20).

wave efficiency sum of squares df mean square F significance probability between treatment groups 1.074 3 0.358 2.393 0.084 treatment group-within 5.384 36 0.150 Sum 6.458 39

As a result of measuring the wave efficiency according to each treatment in the hair bleached three times, the F value between the treatment groups was 2.393 and the significance probability was 0.084, showing no significant difference.

Claims (15)

A cosmetic composition for hair protection and restoration comprising a small molecule derived from silkworm hemolymph as an active ingredient. According to claim 1,
The silkworm hemolymph-derived small molecule is a low-molecular substance of 10 KDa or less, characterized in that, a cosmetic composition for hair protection and restoration. According to claim 1,
A cosmetic composition for hair protection and restoration, characterized in that the silkworm hemolymph-derived small molecule is included in 0.001% to 20% by weight relative to the total weight of the composition. According to claim 1,
A cosmetic composition for protecting and restoring hair, characterized in that it further comprises castor oil. According to claim 4,
Characterized in that the castor oil is included in 0.001% to 20% by weight relative to the total weight of the composition, hair protection and restoration cosmetic composition. According to claim 1,
A cosmetic composition for protecting and restoring hair, characterized in that it improves hair damage caused by permanent. According to claim 6,
The hair damage is a lifting phenomenon of the hair epidermis, loss of hair interstitial matter, a decrease in hair tensile strength or elongation, a decrease in hair moisturizing power, or a combination thereof, characterized in that, a cosmetic composition for hair protection and restoration. According to claim 1,
A cosmetic composition for protecting and restoring hair, characterized in that it enhances the permanent effect of damaged hair. According to claim 6 or 8,
The permanent is a cosmetic composition for hair protection and restoration, characterized in that the permanent by heat treatment. A permanent waving composition comprising a small molecule derived from silkworm hemolymph and castor oil. According to claim 10,
The permanent wave composition, characterized in that the silkworm hemolymph-derived small molecule is included in 0.1% to 20% by weight relative to the total weight of the permanent wave composition. According to claim 10,
The permanent wave composition, characterized in that the castor oil is included in 0.1% to 20% by weight relative to the total weight of the permanent wave composition. According to claim 10,
The permanent wave composition further comprises a first agent as a reducing agent and/or a second agent as an oxidizing agent. A kit for permanent waving, comprising the cosmetic composition for protecting and restoring hair according to any one of claims 1 to 9, a first agent as a reducing agent, and a second agent as an oxidizing agent. Treating hair with low molecular weight derived from silkworm hemolymph and castor oil; and
A cosmetic method for improving permanent efficiency and hair damage, comprising performing a permanent on hair.

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