KR20220141377A - A composition for relieving hangovers containing turmeric and the process for the preparation thereof - Google Patents

Abstract

The present invention relates to a composition for relieving hangover comprising turmeric as an active ingredient, which comprises: 30 to 40 wt % of turmeric, 12 to 16 wt % of Japanese raisin tree, 20 to 30 wt % of milk thistle, 10 to 15 wt % of the root of an arrowroot, 5 to 6 wt % of black garlic, 3 to 5 wt % of Injin mugwort, 3 to 4 wt % of the fruit of Chinese matrimony vine, and 1 to 2 wt % of fruit of schisandra chinensis. The hangover relief pills of the present invention show the maximized synergistic effect in relieving hangover. In particular, in the case of pills, the active ingredient is used in the form of a powder to act as a binder, which has an advantage in the formulation. By using turmeric powder sterilized at high temperature as a main ingredient, color, flavor and overall preference can be greatly improved, and the effect of being able to be safely carried anywhere, anytime, and used usefully without any objection can be provided.

Description

A hangover relieving pill containing turmeric as an active ingredient and a manufacturing method thereof {A composition for relieving hangovers containing turmeric and the process for the preparation thereof}

The present invention relates to a hangover relieving pill containing turmeric as an active ingredient and a method for manufacturing the same, and more specifically, to a mixture of turmeric, Heotgae tree fruit, milk seed extract, brown root, black garlic, wormwood, Goji berry and Schisandra schisandra as an active ingredient. It relates to a method for producing a hangover relief ring with improved preference while exhibiting a maximized synergistic effect in relieving a hangover, characterized in that it comprises, and a ring manufactured thereby.

Due to the complexity and diversity of work due to industrial development, drinking is becoming essential for modern people who have frequent dinner parties and entertainment, and who are stressed by various factors, and as a result, drinking opportunities are significantly increasing.

Alcohol consumption in Korea is at the highest level in the world, and the socioeconomic and health effects of drinking are enormous, and the number of deaths from liver cancer or cirrhosis is the main cause of death. It has been reported that excessive alcohol intake affects almost all parts of the body and causes many diseases such as liver disease, gastritis, pancreatitis, high blood pressure, stroke, esophagitis, diabetes and heart disease.

The hangover phenomenon is caused by the toxic action of ethyl alcohol and/or acetaldehyde accumulated in the liver cells. It means a phenomenon that causes symptoms such as bloating and vomiting. A hangover can usually occur over a period of several hours to several days. The main symptoms include headache, muscle pain, nausea, vomiting, dizziness, poor concentration, dry mouth, fatigue, satiety, hyperexcitability, agitation and depression, and sweating. Changes in sleep patterns and discomfort in the gastrointestinal tract may also be accompanied. Weakness and decreased cognitive ability may be important symptoms of alcohol-induced hangovers.

Due to the characteristics of alcohol metabolism described above, the market for hangover remedies is active mainly in Northeast Asian countries such as Korea, Japan, China, and Taiwan, and various products are being developed actively in these countries.

Research on drugs that can remove hangovers and reduce blood alcohol concentration have been actively conducted, but there is still no drug with clear results.

Ethanol, the main component of alcohol, has a very diverse and extensive effect on the human body physically and mentally, and many studies are being conducted on its metabolic process and toxic expression characteristics. Ingested ethanol is absorbed through the digestive tract, reaching peak blood levels between 20 and 120 minutes after ingestion.

Absorbed ethanol is metabolized in all organs including the liver, and some (about 10%) is excreted through respiration or urine and sweat. When a small amount of alcohol is ingested under normal conditions, the ethanol that enters the liver is converted to acetate by the action of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in the cell cytosol. It is excreted out of hepatocytes through the circulatory system.

In particular, acetaldehyde, the first metabolite of ethanol, has been found to be the main cause of alcoholic liver disorder because it has a very high reactivity and strong toxicity compared to ethanol.

Acetaldehyde interferes with the respiration of mitochondria, an energy generating organ within cells, inhibits oxidative phosphory-lation, and also binds to cell-membrane proteins and collagen proteins to produce antibodies. , has been found to exhibit immunological cytotoxicity and inhibit the release mechanism of hepatocyte-secreted proteins. In addition, acetaldehyde promotes the synthesis of collagen in myofibroblast to cause liver fibrosis and degenerative enlargement of hepatocytes, and also forms adducts by reacting with macromolecules in vivo. In addition, triglycerides are also accumulated in the liver by alcohol ingested into the human body. It is known that the inhibition of fatty acid oxidation by alcohol acts as an important factor in the accumulation of triglycerides in the liver, rather than increasing fatty acid synthesis.

The accumulation of triglycerides in the liver reflects the metabolic disorder of the liver, and the continuous accumulation of triglycerides may eventually lead to the proliferation of fibrous tissue and damage to the hepatocytes.

As a result of this alcohol metabolism, a lot of fatty acids (脂肪酸) are made and fat is accumulated in the liver, which is called alcoholic fatty liver. This alcoholic fatty liver often progresses to chronic liver disease. It has been reported that 10-35% of alcoholic hepatitis and 8-20% of cirrhosis are related to alcoholic fatty liver. In the liver, not only acetaldehyde, but also various types of aldehydes known to be extremely harmful to the living body are generated by the metabolic process of various drugs, fat metabolites, lipid peroxide, and oxidative cleavage of C-C bonds. Aldehyde dehydrogenase, the main enzyme involved in the oxidation of aldehydes including acetaldehyde, is widely distributed in many organs and tissues including liver, kidney, heart and stomach, and also exists in the cytosol, mitochondria, and microsomes to show a wide range of substrate specificities, and various aldehydes It has the properties of a drug metabolizing enzyme that can respond to

Therefore, when the aldehyde dehydrogenase activity in the liver is changed by any factor, it is known that it has an important effect on the detoxification and metabolism of the active aldehyde. In this process, alcohol and acetaldehyde damage liver cells and brain cells, cause vomiting and headache, and in severe cases cause chills or abdominal pain. These physiological phenomena are the cause of hangovers.

And in the case of a person lacking alcohol dehydrogenase, etc., it puts more strain on liver function and interferes with normal metabolism, which makes hangovers more severe. In particular, acetaldehyde dehydrogenase has type II, which initiates oxidation even at low concentrations of acetaldehyde, and type I, which does not act unless acetaldehyde is at a high concentration. Due to the slow and therefore non-oxidized toxic action of acetaldehyde and ethanol, normal metabolism is disturbed, and various hangover phenomena are felt.

Therefore, in order to eliminate a hangover caused by alcohol ingestion and to develop a drug that can reduce blood alcohol concentration, a number of drinks made with herbal or artificial ingredients alone or mixed are being developed. It is often used to relieve hangovers.

However, hangover relieving pills or beverages using these herbal preparations may be toxic to the oriental medicine itself, which may put more strain on the liver. In some cases, it may cause side effects such as general malaise, bloating, vomiting or abdominal pain, so it is still a drug that effectively removes the overall hangover symptom or induces the restoration of the physiological function of the body by protecting and treating the damaged liver. development is insignificant.

Herbal materials mainly used for hangover remedies include sagebrush, radish, wormwood, arrowroot, persimmon leaf, sagebrush, sagebrush, nogeun, heartburn, gold and silver flower, upper leaf, goji berry, cheonmundong, longan meat, gardenia, tosaja, Haeseong tea, green tea, licorice, brown flower, lotus green peel, mokhyang, tangerine peel, ginseng, jeoryeong, baekbokryeong, shinguk, taeksa, dried ginger, baekchul, baekduguin, sine, oligosaccharide, and gourmet.

Currently, as a hangover remover, various drinks including herbal medicines are on the market, and these drinks are used alone after drinking or added to alcoholic beverages before drinking.

The ingredients added to the hangover relieving drink inhibit alcohol absorption, promote alcohol metabolism, reduce blood alcohol concentration, protect liver cells from alcohol, and apply gastrointestinal mucosa to prevent alcohol-induced gastrointestinal mucosa damage and protect the stomach. It is known to exhibit the function of In addition, since it has a strong intestinal deodorizing effect, it removes the odor of alcohol and food after drinking, suppresses the generation of harmful components in the intestine, maintains the intestinal flora, and relieves colon symptoms related to drinking. In addition, it is known to exhibit effects such as removal of intestinal decay products such as ammonia and indole, blood purification, kidney protection, and alleviating alcohol-induced hangover effects to prevent deterioration of physical condition.

On the other hand, turmeric refers to the tuber root of turmeric as it is or after removing the bark and steaming it, and is a perennial plant belonging to the order Ginger. use, etc.

Turmeric is widely used as curry powder and is known to have liver detoxification, anticancer effects, and wound healing effects. In oriental medicine, it is prescribed to treat shoulder pain, menstrual pain, and colic. appears in cases such as

In addition, the characteristic yellow color of curry is a natural color from turmeric, one of the main ingredients, and this color is due to a substance called curcumin. Curcumin is a yellow spice with polyphenols that has been widely used in Indian food extracted from the root of Curcuma longa Linn (Zingiberaceae), a plant belonging to the ginger family from East India. It is a curcuminoid of turmeric. In food additives, it is used as a yellow colorant and as a spice.

Curcumin has anti-tumor, antioxidant, anti-amyloid and anti-inflammatory actions, and the anti-inflammatory action is performed by inhibition of eicosanoid biosynthesis. Curcumin inhibits oxidative DNA damage and lipid peroxidation, and acts as an antioxidant and scavenger.

Accordingly, the inventors of the present inventors continued research to develop a hangover reliever that not only maximizes the hangover relieving effect, but also increases the preference, can be carried in the aircraft, and can be conveniently taken anytime, anywhere. And despite hangover relieving effects, in actual commercialization, turmeric, a root crop, has a high soil microbial contamination level, so it was not sterilized sufficiently. The present invention was completed by preparing a pill that anyone can take without any objection due to improved taste while using it.

The technical problem to be solved in the present invention is to provide a hangover relieving pill containing turmeric as an active ingredient that can be taken conveniently anytime, anywhere as well as increasing the preference while maximizing the hangover relieving effect.

In addition, the technical problem to be solved in the present invention is to provide a method for manufacturing the hangover relief ring.

In order to solve the above technical problem, in the present invention, 30 to 40% by weight of turmeric, 12 to 16% by weight of Heotgae tree fruit, 20 to 30% by weight of milk seed, 10 to 15% by weight of ground root, 5 to 6% by weight of black garlic , Injin mugwort 3 to 5% by weight, Goji berry 3 to 4% by weight, and omija 1 to 2% by weight is characterized in that it is a composition for relieving a hangover.

Preferably, the hangover relieving composition may be a functional food composition, more preferably the composition may be a pill formulation.

In order to solve the other technical problems described above, the present invention provides a method for producing a pill formulation for relieving hangover, characterized in that it comprises the following steps:

In particular, the turmeric powder used in the present invention is thinly spread out in a PET pouch to a thickness of 80 mm so that it is sufficiently sterilized, and then the dried turmeric powder is sterilized at a pressure of 2000 psi and a temperature of 121 degrees in a pressure high-temperature sterilizer for 20 minutes. use.

(S1) Prepare turmeric, huskae tree fruit, milk seed extract, black pepper, black garlic, mugwort mugwort, goji berry and omija powder, respectively, but the turmeric powder is a step;

(S2) The powder prepared in the above step (S1) is 30 to 40 wt% of turmeric, 12 to 16 wt% of Heotgae fruit, 20 to 30 wt% of milk seed, 10 to 15 wt% of ground root, 5 to 6 wt% of black garlic , Injin mugwort 3 to 5% by weight, Goji berry 3 to 4% by weight, and the step of mixing 1 to 2% by weight of Schisandra;

(S3) kneading by adding water to the mixture mixed in step (S2), and then rolling with a roll mill;

(S4) ring-closing the mixture rolled in step (S3) in the form of a ring; and

(S5) drying the ring recirculated in step (S4) at 60 to 70° C. for 7 to 9 hours.

The present invention is a hangover-removing pill containing as an active ingredient a mixture of turmeric, sagebrush fruit, milk seed extract, black root, black garlic, wormwood, goji berry and omija powder as an active ingredient. As the color and fragrance are improved, the preference can be increased, and it has the effect that it can be carried and used usefully without any objection, anytime, anywhere in the aircraft.

In particular, in the case of pill formulation, it has a very advantageous formulation by using powders of turmeric, sagebrush, milk seed extract, black pepper, black garlic, ginseng mugwort, goji berry, and omija powder, which is very advantageous in formulation. Due to its use, color, fragrance, and overall preference can be significantly improved.

The following drawings attached to this specification illustrate preferred embodiments of the present invention, and serve to further understand the technical idea of the present invention together with the above-described content of the invention, so the present invention is limited to the matters described in those drawings It should not be construed as being limited.
1 is a photograph of the active ingredient of the pill formulation for relieving a hangover of the present invention.
Figure 2 is a result of measuring the safety against bacteria of the present hangover relieving pill formulation.
Figure 3 shows the results of measuring the alcohol-induced hepatocellular damage protective effect of the hangover relieving pill of the present invention.
Figure 4 shows the results showing the change in liver weight after administration of the hangover relieving pill of the present invention.
Figure 5 is a photo of a pressure sterilizer having an improved process for sterilization of turmeric powder used in the present pill formulation for hangover relief.

Hereinafter, the present invention will be described in more detail.

In the present invention, 30 to 40% by weight of turmeric, 12 to 16% by weight of Heotgae fruit, 20 to 30% by weight of milk seed, 10 to 15% by weight of ground root, 5 to 6% by weight of black garlic, 3 to 5% by weight of wormwood, Goji berry It provides a composition for relieving a hangover, characterized in that it contains 3 to 4% by weight, and 1 to 2% by weight of omija.

A composition comprising a mixture of turmeric, sagebrush fruit, milk seed extract, black pepper, black garlic, wormwood, goji berry and omija powder according to the present invention exhibits a maximized synergistic effect in relieving hangover.

Turmeric, the active ingredient of the composition for relieving hangovers of the present invention, is widely used as curry powder and is known to have effects such as liver detoxification, anticancer action, and wound healing. In oriental medicine, it is prescribed to treat shoulder pain, menstrual pain, and colic. appears in cases such as The unique yellow color of curry is a natural color from turmeric, one of the main ingredients, and this color is due to a substance called curcumin. Curcumin has anti-tumor, antioxidant, anti-amyloid and anti-inflammatory actions, and the anti-inflammatory action is performed by inhibition of eicosanoid biosynthesis. Curcumin inhibits oxidative DNA damage and lipid peroxidation, and acts as an antioxidant and scavenger.

In the composition for relieving a hangover of the present invention, color, fragrance and overall preference can be greatly improved by using turmeric powder as a main component, but since turmeric is a root crop, many microorganisms are contaminated from the soil, so it may cause deterioration or palatability in the manufacturing process. The problem was solved by sterilizing and using turmeric powder under special conditions.

In order to prevent the deterioration of the turmeric powder and the occurrence of off-flavor in manufacturing the hangover relieving pill containing turmeric as an active ingredient of the present invention, the turmeric pressure sterilization device (see FIG. 5) was improved and the manufacturing process was set through an optimization test, After thinly spreading turmeric powder on a PET pouch to make it 80mm thick, it was sterilized at a pressure of 2000psi and a temperature of 121°C for 20 minutes in a pressurized high-temperature sterilizer to prepare and use dried turmeric powder.

The active ingredient of the composition for relieving hangovers of the present invention, Heotgae tree, is a deciduous broad-leaved tree of the Buckwheat family, and the fruit is effective in alcohol poisoning, urinary discomfort, and vomiting, and the fruit tree is effective in health and nourishment. Gwabyeong and stems are sweet and fragrant, and have been used for food, fruit wine, and medicinal purposes to remove poison. Heotgae tree has the effect of rotting alcohol, and raw juice is excellent for removing hangovers and decomposing alcohol because it relieves alcohol poisoning and stops nausea. In addition, it is effective in protecting the liver function such as jaundice, fatty liver, cirrhosis, gastrointestinal disease and colitis, which are side effects of excessive drinking.

Milk thistle (Silybum marianum), an active ingredient of the composition for relieving hangovers of the present invention, is a kind of plant in the Asteraceae family, and it is known that silymarin extracted from seeds helps liver health. Milk thistle is known to have strong antioxidant effects, such as the effect of repairing liver cells caused by alcohol or other toxic substances, and preventing the destruction of new hepatocytes by toxins and reducing inflammation.

The active ingredient of the composition for relieving hangovers of the present invention, galgeun, refers to the tuber of kudzu (Pueraria thunbergiana BENTH), a perennial mangyeong plant belonging to the leguminosae family. Puerarin, puerarin xylose ), daidzein, sitosterol, etc. are contained, and are known to have blood flow increasing action, antispasmodic action, antipyretic action, and the like.

Black garlic, which is the active ingredient of the composition for relieving hangovers of the present invention, is obtained by aging garlic. Allicin contained in garlic is decomposed and does not generate a strong flavor inherent in garlic. By preventing the conversion to allicin by

Artemisia capillaris, an active ingredient of the composition for relieving hangovers of the present invention, is a herbaceous perennial plant of the Compositae family, which is a plant that grows wild in Korea, and is also referred to as scorched wormwood. Injin mugwort mainly uses young shoots and is known to be effective in anti-inflammatory, antibacterial, anti-acne, antioxidant, diuretic, and jaundice. The main components include various components from chlorogenic acid and caffeic acid, which are types of polyphenols, to oleic acid and linoleic acid, which are essential oil components.

Goji berry, the active ingredient of the composition for relieving hangover of the present invention, is a fruit of Goji berry belonging to the family Gajitaceae. It contains betaine, which is effective for the prevention and treatment of fatty liver, and contains vitamin C and rutin to strengthen blood vessels and lower blood pressure. It is also effective in treatment.

Omija, the active ingredient of the composition for relieving hangovers of the present invention, is a fruit that grows on a deciduous deciduous tree of the Omija family. Omija is generally known to have the effects of improving blood flow, preventing hypertension, stroke, cardiovascular disease, improving immunity, preventing diabetes, improving liver function, restoring energy, and improving respiratory diseases.

According to one embodiment of the present invention, the hangover relieving composition may be a functional food composition.

The food composition for relieving a hangover according to the present invention may be prepared in a form in which the active ingredient is incorporated into a foodologically acceptable carrier.

In the case of the formulation, it may be prepared using a diluent or excipient such as a commonly used filler, extender, binder, wetting agent, disintegrant, and surfactant. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and such solid preparations include at least one excipient in the active ingredient, for example, starch, calcium carbonate, sucrose, lactose, gelatin. It can be prepared by mixing, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid formulations for oral administration include suspensions, solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used diluents, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. can

According to one embodiment of the present invention, the hangover relieving agent of the present invention may be formulated in the form of a pill formulation.

The dosage of the hangover relieving agent according to the present invention is selected in consideration of the individual's age, weight, sex, physical condition, and the like.

According to one embodiment of the present invention, the pill formulation for relieving a hangover of the present invention can be conveniently taken before or after drinking.

The present invention provides a method for preparing a pill formulation for relieving hangover, comprising the following steps:

(S1) preparing the powder of turmeric, Heotgae tree fruit, milk seed extract, garlic root, black garlic, wormwood, Goji berries and Schisandra;

(S2) 30 to 40% by weight of turmeric prepared in the step (S1), 12 to 16% by weight of hemp seeds, 20 to 30% by weight of milk seed extract, 10 to 15% by weight of ground root, 5 to 6% by weight of black garlic, Injin mugwort 3 to 5% by weight, 3 to 4% by weight of Goji berry, and 1 to 2% by weight of Schisandra;

(S3) kneading by adding water to the mixture mixed in step (S2), and then rolling with a roll mill;

(S4) ring-closing the mixture rolled in step (S3) in the form of a ring; and

(S5) drying the ring recirculated in step (S4) at 60 to 70° C. for 7 to 9 hours.

According to one embodiment of the present invention, by using each of turmeric, honeysuckle fruit, milk seed extract, black root, black garlic, wormwood, goji berry, and omija in powder form, it serves as a binder, and formulation has a very advantageous advantage.

According to one embodiment of the present invention, the hangover relieving composition may be a functional food composition.

As described above, the composition comprising a mixture of turmeric, honeysuckle tree fruit, milk seed extract, black garlic, ginseng wormwood, goji berry and omija powder according to the present invention exhibits a maximized synergistic effect in relieving a hangover. In particular, in the case of pills, it has a very advantageous formulation by acting as a binder by using powders of turmeric, sagebrush, milk seed extract, black root, black garlic, wormwood, goji berries and omija. Fragrance and overall palatability can be significantly improved.

Therefore, as an active ingredient, it is a pill preparation for hangover that contains a mixture of turmeric, sagebrush fruit, milk seed extract, black pepper, black garlic, wormwood, Goji berry and omija powder of the present invention as an active ingredient. It is expected that it will be able to be used effectively without any repulsion by carrying it anywhere, anytime, even in the dusty air, as the color and fragrance are improved and the preference can be increased.

Hereinafter, examples and the like will be described in detail to help the understanding of the present invention. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

<Example 1> Preparation of pills for hangover relief

As shown in Figure 1, each of the active ingredients of the pill formulation for relieving a hangover of the present invention was obtained. 1 is a photograph of the active ingredient of the pill formulation for relieving a hangover of the present invention.

In order to prevent the deterioration of the turmeric powder and the occurrence of off-flavor in manufacturing the pill for relieving hangover containing turmeric as an active ingredient of the present invention, the turmeric pressure sterilization device was improved and the production process was set through an optimization test, turmeric in a PET 12mm pouch After spreading the powder thinly to a thickness of 80 mm, and then sterilizing the powder at a pressure of 2000 psi and a temperature of 121 ° C for 20 minutes in a pressurized high-temperature sterilizer, dried turmeric powder was prepared and used.

Composition for relieving hangover containing turmeric as an active ingredient and method for preparing the same

As shown in Table 1 below, 35% by weight of turmeric powder, 14% by weight of mulberry fruit powder, 25% by weight of milk thistle powder, 12% by weight of ground root powder, 5.6% by weight of black garlic powder, 4% by weight of wormwood powder, wolfberry After mixing 3.2 wt% of powder, and 1.2 wt% of Schisandra Schisandra, kneaded and rolled with a roll mill, then made into pills and dried at 65° C. for 8 hours to prepare a pill formulation for relieving hangovers.

Composition for relieving hangover containing turmeric as an active ingredient and method for preparing the same

Mixing ratio (wt%) hawthorn fruit 14 craving 12 black garlic 5.6 wormwood 4 goji berries 3.2 Schisandra 1.2 turmeric powder 35 milk thistle 25 total 100

<Test Example 1> Physicochemical experiment of pills for hangover relief

(1) Chromaticity (Lab value)

The chromaticity of the sample was calculated as the average value of L ^* , a ^* , b ^* after three repeated measurements using a spectrophotometer (SPECTROPHOTOMETER CM-5).

L ^* : Represents reflectance (brightness similar to human vision), and can be expressed in units of less than a decimal point in steps from 0 to 100.

a ^* : In the chromaticity diagram, +a ^* indicates the direction of red and -a ^* indicates the direction of green.

b ^* : In the chromaticity diagram, +b ^* indicates yellow and -b ^* indicates blue direction.

(2) moisture content

3 g of the sample was measured by the loss-on-drying method, and arithmetic was performed according to Equation 1 below.

① Refer to Food Codex No. 9 General Test Method 1.1.1.1 Loss on drying method

② Precisely weigh 3~5 g of sample on a weighing dish made with constant weight by heating

③ For each food, put it in a dryer at the prescribed temperature and dry it for 3 to 5 hours

④ Cool for about 30 minutes in a desiccator and weigh

⑤ Dry the weighing dish for 1-2 hours and repeat the same operation until it becomes constant weight

(3) Water activity

Using a water activity measuring instrument (AQS-31, human corporation), half of the sample was placed in a plastic container and the average value was calculated after three repeated measurements.

(4) Measurement of physical properties

Hardness (g) was measured three times using a food property analyzer (Texture Analyzer, TAXTplus, Stable Micro System), and then the average value was calculated.

division result value chromaticity L (brightness) : 40.08
a (red): 11.56
b (yellow): 28.66 moisture (%) 2.98 water activity 0.468 Hardness (g) 11543 ±1840

As shown in Table 2 above, in the case of chromaticity, the b value (yellow) was measured to be 28.66. This is judged to be due to the characteristics of the turmeric powder contained in the hangover relieving pills, and the moisture content was 2.98% and the water activity was 0.468, which was low to match the characteristics of the dried pills. In addition, as a result of physical property measurement, the hardness of the ring was measured to be 11543 g.

<Test Example 2> Bacterial safety measurement of hangover relieving pills

(1) E. coli, coliform group

1 mL of the test solution and 1 mL of each 10-fold dilution solution were inoculated on dry film medium for E. coli and coliform groups, and then cultured at 35-37°C for 24-48 hours to confirm the presence of E. coli and coliform groups.

(2) General bacteria

After inoculating 1 mL of the test solution and 1 mL of each 10-fold dilution on a dry film medium for bacterial count, incubate at 35-37°C for 24-48 hours to calculate the resulting number of red colonies and multiply the average number of colonies by the dilution factor. Calculated.

(3) yeast, mold

Inoculate 1 mL of the test solution and 1 mL of each 10-fold dilution in yeast/fungi dry film medium and incubate at 25~27℃ for 36~60 hours. For yeast, the cells were small and greenish, and for molds, the cells with broad and unclear nuclei and edges were counted and calculated by multiplying the average number of colonies by the dilution factor.

The standard specifications for processed agricultural products for each bacteria are as follows.

- Coliform group: n=5, c=1, m=0, M=10 (limited to sterilized products)

- Number of bacteria: n=5, c=0, m=0 (limited to sterilized products)

above,

n is the number of iterations of the experiment after collecting a random sample from one lot,

c is the maximum allowable number of samples exceeding the microbial count standard in one assay unit,

m is the maximum number of viable cells per g or a specific number of bacteria (minimum),

M is the number of bacteria (Maximum) that is the criterion for determining rejection and condition pass.

Figure 2 is a result of measuring the safety of the pill formulation for hangover relief against bacteria. As shown in FIG. 2, as a result of the microbial test of the hangover relieving product, E. coli, coliform group, yeast, and mold were not detected, and in the case of general bacteria, it can be determined that microbial safety has been secured by detecting a very small amount.

<Test Example 3> Measurement of the hepatocellular damage protective effect of a pill formulation for relieving hangover

Sprague-Dawley rats (male) with an average weight of 170-180 g (obtained from: Samtaco BIO KOREA) were allowed to freely ingest solid samples and tap water, and were used after preliminary breeding for 1 week. The conditions of the animal room were temperature: 22±2°C, humidity: 50±5%, and contrast: 12 hours light/dark cycle.

After excluding individuals with abnormalities during the acclimatization period and individuals who did not gain weight normally, group separation was performed according to Table 3 below so that the average weight and standard deviation between each group were uniform. After group separation, the remaining animals were maintained until the start date of administration, after which they were euthanized. For the control group, the dose concentration (6.2 ml/kg) of Dyeongyeong 808 was converted based on the market dose of Dyeongyeong 808 per serving (140 ml), and the requested test substance was converted based on the assumption that an adult dose of 3g was used. degrees (300 mg).

After a one-week acclimatization period, the test substance was repeatedly administered once a day for a total of 3 days. Complex powder (hangover pill of Example 1) 1, 2 and 3 test groups were administered orally. In order to equalize the dose and stress induced by administration to each test group, the same amount of physiological saline as the control group, negative control group and positive control group, and complex powder 1, 2 and 3 administration groups was orally administered. One hour after oral administration, alcohol was orally administered.

(1) Measurement of alcohol-induced hepatocellular injury protective effect (in vitro)

After seeding the 96-well plate so that the number of cells became 104-106 cell/ml, 24 hours later, samples were treated at various concentrations. 24 hours after sample treatment, MTT (3-[4,5-dimethylthiazole-2-yl] Cell viability was measured by -2,5-diphenyl- tetrazolium bromide (Sigma, USA) assay. MTT solution (5 mg/mL) corresponding to one-tenth of the medium was added, and further cultured for 4 h at 37 °C, 5% CO2 incubator to reduce MTT to form formazan. Then, the medium was removed so that the formazan was not lost, and after drying for 30 min in the dark, DMSO was added, stirred for 10 min, and the absorbance value was measured at 540 nm using an ELISA reader.

① Check the toxicity of the sample to determine the concentration of the sample

For the purpose of the experiment to determine the treatment concentration of the composite powder to measure the alcohol-induced liver damage protective effect, a test for determining the safe concentration range of the composite powder itself was conducted.

The complex powder was treated with primary cultured cells of hepatocytes extracted from rats at concentrations of 0.01, 0.03, 0.1, 0.3, 1, 3, 10, 30, 100 μl/ml, and 24 hours later, as a result of checking by MTT, 1 μl Cytotoxicity was observed in the complex powder at /ml, and high toxicity was observed at concentrations of 30 and 100 μl/ml.

Based on this, the appropriate treatment concentration for the alcohol-induced cytotoxicity test was determined to be 1 μl/ml or less.

② Confirmation of sample toxicity to determine the concentration of the control sample (Dawn 808)

Dyeing 808, which was used as a control group, was treated with hepatocytes extracted from rats at concentrations of 0.02, 0.2, and 2 μl/ml in primary cultured cells, and 24 hours later, as a result of MTT, no cytotoxicity was observed. Based on this, an appropriate treatment concentration for the alcohol-induced cytotoxicity test was determined. The requested complex powder and Dawn808 obtained non-toxic results within a similar concentration range.

③ Measurement of the protective effect of alcohol-induced hepatocellular damage

Hepatocytes extracted from rats were pre-treated with the complex powder for 2 hours, treated with alcohol (200 mM), sealed to prevent evaporation, and cultured for 24 hours. Cell viability was measured by MTT assay. .

Figure 3 is a result of measuring the alcohol-induced hepatocellular damage protective effect of the hangover relieving pill of the present invention.

As shown here, it was confirmed that the MTT value was significantly decreased by the alcohol treatment, thereby induced cytotoxicity by alcohol. Under these conditions, the level of MTT by the pretreatment of the composite powder increased significantly in a concentration-dependent manner. At a low concentration of 0.01 μl/ml, it showed a rather low significant difference (p<0.01) and protected hepatocytes, but as the concentration increased, it was observed that the alcohol-induced liver damage was highly protected. Dawn808, which was used as a control, exhibited about 40% hepatoprotective effect, and it was observed that it exhibited a superior hepatocellular protective effect than the requested test substance, which exhibited about 30% hepatoprotective effect.

The hepatoprotective effect of the complex powder against alcohol was confirmed in vitro, and based on this, a study was conducted to verify the effect in vivo.

(2) Measurement of hangover relieving ability (in vivo)

① How to measure blood alcohol concentration

Ethanol is oxidized by a liver enzyme called alcohol dehydrogenase (ADH) to form acetaldehyde. In this process, NADH is produced with the help of a coenzyme called NAD+, and the amount of the product is measured at absorbance at 340 nm. Blood alcohol concentration was calculated according to Equation 2 below.

(i) Mix 0.1ml of blood with 3ml of reaction mixture (potasium phosphate buffer pH 9 + NAD+ tablet).

(ii) incubation at 20°C for 3 min.

(iii) absorbance measurement at 340 nm (A1)

(iv) Add 0.05ml of ADH (alcohol dehydrogenase).

(v) incubation at 20 °C for 5 min

(vi) absorbance measurement at 340 (A2)

Immediately after oral administration of the sample (0 minutes), after alcohol treatment, blood was collected from the tail for 30 minutes, 1, 2, 4, and 8 hours, and centrifuged at 4,000 rpm for 20 minutes to separate the serum, followed by an Ethanol colorimetric assay kit (Biovision). ) was used to measure blood alcohol concentration (ng).

As a result, the alcohol concentration in the alcohol-administered group showed the highest value at 1 hour after administration, and then showed a tendency to decrease slowly. After that, a rapidly decreasing trend was observed. In the low concentration group, the blood alcohol concentration was slightly lower than in the alcohol-only group, but no statistical significance was observed. In the intermediate concentration treatment group, the highest blood alcohol concentration was observed 2 hours after alcohol administration. In addition, as the time for increasing the alcohol level is delayed, it can be seen that damage to alcohol can be suppressed as the absorption of alcohol into the blood is delayed. It was observed that the level of Dawn 808, which was used as a high dose group and a control group, did not increase significantly after alcohol administration, and also maintained a consistently low level.

After 1 hour after alcohol administration, it was confirmed that the blood alcohol concentration was more significantly lower than that of the Dawn 808 administration group by administration of the high-concentration pill prototype.

As a result, it was found that at the time of the highest blood alcohol concentration, the alcohol concentration of 808 at life 808 decreased by 29.7%, at the low concentration by 13.6%, at the medium concentration by 18.98%, and at the high concentration by 28.86%. After 8 hours, it was found that the alcohol concentration decreased by 17.99% in Dawn 808, 10.98% in low concentration, 22.1% in medium concentration, and 18.37% in high concentration.

② Measurement of enzyme activity in liver or blood

The enzyme source for alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activity is homogenized by adding 4 times the amount of ice-cold 0.25 M sugar solution to a certain amount of liver tissue or blood of the extracted animal and then homogenizing it. The supernatant (postmitochondrial fraction, PMF) and mitochondrial fraction obtained by centrifugation at × for 20 minutes were used. - Alcohol dehydrogenase (ADH) activity was determined by adding ethanol and nicotinic acid amide as a substrate according to Bernet & Bergermeyer's method (Bernet E: Methods of enzymatic analysis. Vch Pub; 3rd edition pp. 1506-1509, 1983). The content of nicotinamide adenine dinucleotide produced during the period was measured at a wavelength of 340 nm, and the activity was expressed in nmole as the amount of nicotinamide adenine dinucleotide produced by 1 mg of protein per minute.

The activity of aldehyde dehydrogenase (ALDH) was determined by Lundquist's method (Bernet E: Methods of enzymatic analysis. Vch Pub; 3rd edition pp. 1, 1983). Nicotine produced during reaction with the addition of acetaldehyde and nicotinic acid amide as substrates The content of amide adenine dinucleotide was measured at a wavelength of 340 nm, and the activity was expressed in nmole as the amount of nicotinamide adenine dinucleotide produced by 1 mg of protein per minute.

As a result, as a result of measuring alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), the activity of the enzyme decreased by alcohol administration, and the enzyme activity was significantly reduced in the medium and high concentration groups of the drug test product. was observed to be maintained, and it was confirmed that the activity was significantly superior to that of 808 in ALDH activity.

The degree of increase in ADH activity was observed to be 2.26-fold in lifespan (YM), 1.9-fold in low concentration, 2.14-fold in medium concentration, and 2.48-fold in high concentration. As for the degree of increase in ALDH activity, it was observed that lifespan (YM) increased by 2.27 times, at the low concentration by 1.35 times, at the middle concentration by 2.26 times, and at the high concentration by 3.76 times.

③ Toxicity evaluation due to the administration of a test product (animal administration toxicity)

Figure 4 is a graph showing the change in liver weight after administration of the hangover relieving pill of the present invention. As a result of administering ethanol to the experimental animals, it was observed that the weight of the liver increased significantly (p<0.05), and it was found that the weight of the liver was also significantly increased in Dawn808, which was used as a control.

From this result, it can be inferred that Dawn 808 was effective in lowering the blood alcohol level, but the protective effect on liver damage was negligible. In addition, although Dawn808 had the effect of reporting alcohol-induced liver damage in vitro, no significant protective effect against liver damage was observed in the results of administration to animals. This is a result that can be caused by a variety of causes, and it is thought that when administered to an animal, the administered substance is metabolized through various routes, and its effect is reduced.

On the other hand, in the treatment group of the hangover relief pill of the present invention, it was observed that the increase in the weight of the liver was inhibited significantly (p <0.05) in the high concentration treatment group.

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (3)

Method for producing a pill formulation for relieving hangover, comprising the following steps:
(S1) preparing each powder of turmeric, sagebrush fruit, milk seed extract, galgeun, black garlic, wormwood, goji berries and omija;
(S2) The powder prepared in step (S1) is 30 to 40% by weight of turmeric, 12 to 16% by weight of hemp fruit, 20 to 30% by weight of milk seed, 10 to 15% by weight of ground root, 5 to 6% by weight of black garlic , Injin mugwort 3 to 5% by weight, Goji berry 3 to 4% by weight, and the step of mixing 1 to 2% by weight of Schisandra;
(S3) kneading by adding water to the mixture mixed in step (S2), and then rolling with a roll mill;
(S4) ring-closing the mixture rolled in step (S3) in the form of a ring; and
(S5) drying the ring recirculated in step (S4) at 60 to 70° C. for 7 to 9 hours. The method of claim 1,
Turmeric powder is a hangover relieving pill, characterized in that it is turmeric powder that has been sufficiently sterilized and dried through a process of sterilizing at a pressure of 2000 psi and a temperature of 121° C. A method for preparing the formulation. A pill formulation for hangover relief prepared by the method of claim 1 or 2

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