KR20220112447A - Composition for improving skin, comprising Elaeaqnus macrophylla Extract as effective components, Cosmetic composition and Food functional composition including the same - Google Patents
Composition for improving skin, comprising Elaeaqnus macrophylla Extract as effective components, Cosmetic composition and Food functional composition including the same Download PDFInfo
- Publication number
- KR20220112447A KR20220112447A KR1020210016016A KR20210016016A KR20220112447A KR 20220112447 A KR20220112447 A KR 20220112447A KR 1020210016016 A KR1020210016016 A KR 1020210016016A KR 20210016016 A KR20210016016 A KR 20210016016A KR 20220112447 A KR20220112447 A KR 20220112447A
- Authority
- KR
- South Korea
- Prior art keywords
- composition
- skin
- extract
- barley
- present
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 114
- 239000000284 extract Substances 0.000 title claims abstract description 47
- 239000002537 cosmetic Substances 0.000 title claims description 30
- 230000006872 improvement Effects 0.000 claims abstract description 23
- 230000036542 oxidative stress Effects 0.000 claims abstract description 13
- 210000003491 skin Anatomy 0.000 claims description 53
- 241000209219 Hordeum Species 0.000 claims description 35
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 35
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 29
- 239000003642 reactive oxygen metabolite Substances 0.000 claims description 21
- 238000009472 formulation Methods 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 18
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 18
- 229940069780 barley extract Drugs 0.000 claims description 14
- 239000000843 powder Substances 0.000 claims description 13
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 12
- 239000003963 antioxidant agent Substances 0.000 claims description 11
- 230000003078 antioxidant effect Effects 0.000 claims description 11
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims description 10
- 235000007164 Oryza sativa Nutrition 0.000 claims description 10
- 230000036541 health Effects 0.000 claims description 10
- 239000003960 organic solvent Substances 0.000 claims description 10
- 235000009566 rice Nutrition 0.000 claims description 10
- 239000004480 active ingredient Substances 0.000 claims description 9
- 239000000839 emulsion Substances 0.000 claims description 9
- 235000011187 glycerol Nutrition 0.000 claims description 9
- 210000004927 skin cell Anatomy 0.000 claims description 9
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 8
- 235000013376 functional food Nutrition 0.000 claims description 8
- 230000037303 wrinkles Effects 0.000 claims description 8
- 230000003712 anti-aging effect Effects 0.000 claims description 7
- 235000013361 beverage Nutrition 0.000 claims description 6
- 239000006210 lotion Substances 0.000 claims description 6
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 6
- 239000004094 surface-active agent Substances 0.000 claims description 6
- 230000002633 protecting effect Effects 0.000 claims description 5
- 239000006071 cream Substances 0.000 claims description 4
- 239000012046 mixed solvent Substances 0.000 claims description 4
- 230000003213 activating effect Effects 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 239000002775 capsule Substances 0.000 claims description 3
- 210000004709 eyebrow Anatomy 0.000 claims description 3
- 239000000499 gel Substances 0.000 claims description 3
- 239000008187 granular material Substances 0.000 claims description 3
- 239000006072 paste Substances 0.000 claims description 3
- 239000000344 soap Substances 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 239000003826 tablet Substances 0.000 claims description 3
- 230000004898 mitochondrial function Effects 0.000 claims description 2
- 230000009467 reduction Effects 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 2
- 230000002708 enhancing effect Effects 0.000 claims 1
- 108020005196 Mitochondrial DNA Proteins 0.000 abstract description 20
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 11
- 239000001301 oxygen Substances 0.000 abstract description 11
- 229910052760 oxygen Inorganic materials 0.000 abstract description 11
- 230000006820 DNA synthesis Effects 0.000 abstract description 6
- 241001413797 Elaeagnus macrophylla Species 0.000 abstract 2
- 230000030833 cell death Effects 0.000 abstract 1
- 230000002500 effect on skin Effects 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 abstract 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 52
- 210000002950 fibroblast Anatomy 0.000 description 25
- 210000004027 cell Anatomy 0.000 description 20
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 16
- 238000011282 treatment Methods 0.000 description 14
- 238000004519 manufacturing process Methods 0.000 description 13
- 239000013642 negative control Substances 0.000 description 12
- 238000012360 testing method Methods 0.000 description 11
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 210000003470 mitochondria Anatomy 0.000 description 10
- 235000006708 antioxidants Nutrition 0.000 description 9
- 230000003833 cell viability Effects 0.000 description 9
- 241000209094 Oryza Species 0.000 description 8
- 229960005070 ascorbic acid Drugs 0.000 description 8
- 235000010323 ascorbic acid Nutrition 0.000 description 8
- 239000011668 ascorbic acid Substances 0.000 description 8
- 230000006870 function Effects 0.000 description 8
- 230000002438 mitochondrial effect Effects 0.000 description 8
- 230000002407 ATP formation Effects 0.000 description 7
- -1 cell membranes Proteins 0.000 description 7
- 239000004615 ingredient Substances 0.000 description 7
- 230000002829 reductive effect Effects 0.000 description 7
- 230000032683 aging Effects 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 238000000605 extraction Methods 0.000 description 6
- 239000000796 flavoring agent Substances 0.000 description 6
- 235000013355 food flavoring agent Nutrition 0.000 description 6
- 239000003921 oil Substances 0.000 description 6
- 239000008213 purified water Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 230000004913 activation Effects 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 230000001120 cytoprotective effect Effects 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 230000009759 skin aging Effects 0.000 description 5
- 235000013599 spices Nutrition 0.000 description 5
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 4
- 230000002421 anti-septic effect Effects 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000012790 confirmation Methods 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 4
- 230000001747 exhibiting effect Effects 0.000 description 4
- 239000003205 fragrance Substances 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 4
- 239000012679 serum free medium Substances 0.000 description 4
- PRAKJMSDJKAYCZ-UHFFFAOYSA-N squalane Chemical compound CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 description 4
- 239000003381 stabilizer Substances 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 229940058015 1,3-butylene glycol Drugs 0.000 description 3
- VFNKZQNIXUFLBC-UHFFFAOYSA-N 2',7'-dichlorofluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(Cl)=C(O)C=C1OC1=C2C=C(Cl)C(O)=C1 VFNKZQNIXUFLBC-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 3
- 201000004624 Dermatitis Diseases 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 235000019437 butane-1,3-diol Nutrition 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 239000003086 colorant Substances 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 239000000469 ethanolic extract Substances 0.000 description 3
- 239000003906 humectant Substances 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 239000003755 preservative agent Substances 0.000 description 3
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 3
- 210000004761 scalp Anatomy 0.000 description 3
- 210000001626 skin fibroblast Anatomy 0.000 description 3
- 238000003809 water extraction Methods 0.000 description 3
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 2
- 206010003645 Atopy Diseases 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 231100000002 MTT assay Toxicity 0.000 description 2
- 238000000134 MTT assay Methods 0.000 description 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 2
- 229920001214 Polysorbate 60 Polymers 0.000 description 2
- 206010040954 Skin wrinkling Diseases 0.000 description 2
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 2
- 239000006096 absorbing agent Substances 0.000 description 2
- 230000006907 apoptotic process Effects 0.000 description 2
- 230000032677 cell aging Effects 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 231100000433 cytotoxic Toxicity 0.000 description 2
- 230000001472 cytotoxic effect Effects 0.000 description 2
- 231100000135 cytotoxicity Toxicity 0.000 description 2
- 230000003013 cytotoxicity Effects 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 210000004177 elastic tissue Anatomy 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 235000015203 fruit juice Nutrition 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 2
- JXTPJDDICSTXJX-UHFFFAOYSA-N n-Triacontane Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC JXTPJDDICSTXJX-UHFFFAOYSA-N 0.000 description 2
- 230000001590 oxidative effect Effects 0.000 description 2
- 230000019612 pigmentation Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 229920002545 silicone oil Polymers 0.000 description 2
- 230000037394 skin elasticity Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000000600 sorbitol Substances 0.000 description 2
- 235000010356 sorbitol Nutrition 0.000 description 2
- 229940032094 squalane Drugs 0.000 description 2
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 2
- 239000002562 thickening agent Substances 0.000 description 2
- 239000004034 viscosity adjusting agent Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000000080 wetting agent Substances 0.000 description 2
- OZFAFGSSMRRTDW-UHFFFAOYSA-N (2,4-dichlorophenyl) benzenesulfonate Chemical compound ClC1=CC(Cl)=CC=C1OS(=O)(=O)C1=CC=CC=C1 OZFAFGSSMRRTDW-UHFFFAOYSA-N 0.000 description 1
- UUTKICFRNVKFRG-WDSKDSINSA-N (4R)-3-[oxo-[(2S)-5-oxo-2-pyrrolidinyl]methyl]-4-thiazolidinecarboxylic acid Chemical compound OC(=O)[C@@H]1CSCN1C(=O)[C@H]1NC(=O)CC1 UUTKICFRNVKFRG-WDSKDSINSA-N 0.000 description 1
- FFJCNSLCJOQHKM-CLFAGFIQSA-N (z)-1-[(z)-octadec-9-enoxy]octadec-9-ene Chemical compound CCCCCCCC\C=C/CCCCCCCCOCCCCCCCC\C=C/CCCCCCCC FFJCNSLCJOQHKM-CLFAGFIQSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- PXEZTIWVRVSYOK-UHFFFAOYSA-N 2-(3,6-diacetyloxy-2,7-dichloro-9h-xanthen-9-yl)benzoic acid Chemical compound C1=2C=C(Cl)C(OC(=O)C)=CC=2OC2=CC(OC(C)=O)=C(Cl)C=C2C1C1=CC=CC=C1C(O)=O PXEZTIWVRVSYOK-UHFFFAOYSA-N 0.000 description 1
- AZKSAVLVSZKNRD-UHFFFAOYSA-M 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Chemical compound [Br-].S1C(C)=C(C)N=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=CC=C1 AZKSAVLVSZKNRD-UHFFFAOYSA-M 0.000 description 1
- 231100000582 ATP assay Toxicity 0.000 description 1
- 208000002874 Acne Vulgaris Diseases 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 108010011485 Aspartame Proteins 0.000 description 1
- DKPFZGUDAPQIHT-UHFFFAOYSA-N Butyl acetate Natural products CCCCOC(C)=O DKPFZGUDAPQIHT-UHFFFAOYSA-N 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- 108010072220 Cyclophilin A Proteins 0.000 description 1
- 108010068682 Cyclophilins Proteins 0.000 description 1
- 102000001493 Cyclophilins Human genes 0.000 description 1
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 239000004386 Erythritol Substances 0.000 description 1
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 description 1
- 108090000371 Esterases Proteins 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 239000001512 FEMA 4601 Substances 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000004378 Glycyrrhizin Substances 0.000 description 1
- 101000921810 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) Probable cytochrome oxidase subunit 2 Proteins 0.000 description 1
- 102100039869 Histone H2B type F-S Human genes 0.000 description 1
- 101001035372 Homo sapiens Histone H2B type F-S Proteins 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 108091093105 Nuclear DNA Proteins 0.000 description 1
- 229920002230 Pectic acid Polymers 0.000 description 1
- 102100034539 Peptidyl-prolyl cis-trans isomerase A Human genes 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- HELXLJCILKEWJH-SEAGSNCFSA-N Rebaudioside A Natural products O=C(O[C@H]1[C@@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)[C@@]1(C)[C@@H]2[C@](C)([C@H]3[C@@]4(CC(=C)[C@@](O[C@H]5[C@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@H](O)[C@@H](CO)O5)(C4)CC3)CC2)CCC1 HELXLJCILKEWJH-SEAGSNCFSA-N 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 244000228451 Stevia rebaudiana Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000001153 anti-wrinkle effect Effects 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 239000000605 aspartame Substances 0.000 description 1
- 235000010357 aspartame Nutrition 0.000 description 1
- IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 description 1
- 229960003438 aspartame Drugs 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000002981 blocking agent Substances 0.000 description 1
- 235000014171 carbonated beverage Nutrition 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 239000003974 emollient agent Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- HELXLJCILKEWJH-UHFFFAOYSA-N entered according to Sigma 01432 Natural products C1CC2C3(C)CCCC(C)(C(=O)OC4C(C(O)C(O)C(CO)O4)O)C3CCC2(C2)CC(=C)C21OC(C1OC2C(C(O)C(O)C(CO)O2)O)OC(CO)C(O)C1OC1OC(CO)C(O)C(O)C1O HELXLJCILKEWJH-UHFFFAOYSA-N 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 235000019414 erythritol Nutrition 0.000 description 1
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 description 1
- 229940009714 erythritol Drugs 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000004088 foaming agent Substances 0.000 description 1
- 239000003349 gelling agent Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 229940075529 glyceryl stearate Drugs 0.000 description 1
- LPLVUJXQOOQHMX-UHFFFAOYSA-N glycyrrhetinic acid glycoside Natural products C1CC(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2=O)C(O)=O)C)(C)CC2)(C)C2C(C)(C)C1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O LPLVUJXQOOQHMX-UHFFFAOYSA-N 0.000 description 1
- 229960004949 glycyrrhizic acid Drugs 0.000 description 1
- UYRUBYNTXSDKQT-UHFFFAOYSA-N glycyrrhizic acid Natural products CC1(C)C(CCC2(C)C1CCC3(C)C2C(=O)C=C4C5CC(C)(CCC5(C)CCC34C)C(=O)O)OC6OC(C(O)C(O)C6OC7OC(O)C(O)C(O)C7C(=O)O)C(=O)O UYRUBYNTXSDKQT-UHFFFAOYSA-N 0.000 description 1
- 235000019410 glycyrrhizin Nutrition 0.000 description 1
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 229940093915 gynecological organic acid Drugs 0.000 description 1
- 230000003779 hair growth Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000008810 intracellular oxidative stress Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000008099 melanin synthesis Effects 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 230000004065 mitochondrial dysfunction Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000010627 oxidative phosphorylation Effects 0.000 description 1
- LCLHHZYHLXDRQG-ZNKJPWOQSA-N pectic acid Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)O[C@H](C(O)=O)[C@@H]1OC1[C@H](O)[C@@H](O)[C@@H](OC2[C@@H]([C@@H](O)[C@@H](O)[C@H](O2)C(O)=O)O)[C@@H](C(O)=O)O1 LCLHHZYHLXDRQG-ZNKJPWOQSA-N 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000003169 placental effect Effects 0.000 description 1
- 210000005059 placental tissue Anatomy 0.000 description 1
- 239000010318 polygalacturonic acid Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Chemical class 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 235000019203 rebaudioside A Nutrition 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000008263 repair mechanism Effects 0.000 description 1
- 235000019204 saccharin Nutrition 0.000 description 1
- CVHZOJJKTDOEJC-UHFFFAOYSA-N saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 description 1
- 229940081974 saccharin Drugs 0.000 description 1
- 239000000901 saccharin and its Na,K and Ca salt Substances 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 210000002374 sebum Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000003352 sequestering agent Substances 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 230000008591 skin barrier function Effects 0.000 description 1
- 230000005808 skin problem Effects 0.000 description 1
- 230000036560 skin regeneration Effects 0.000 description 1
- 229960002920 sorbitol Drugs 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
본 발명은 보리밥나무 추출물을 유효성분으로 함유하는 피부개선용 조성물, 이를 포함하는 피부 개선용 화장료 조성물 및 건강기능식품 조성물에 관한 것이다.The present invention relates to a composition for improving skin containing a barley extract as an active ingredient, a cosmetic composition for skin improvement and a health functional food composition comprising the same.
피부는 노화 정도를 가장 먼저 판단할 수 있는 요소이므로 많은 사람들이 다양한 방법으로 피부 노화를 막기 위해 노력하고 있다. 특히 자외선, 스트레스, 환경 오염 물질 등을 통해 생성되는 활성산소 (Reactive Oxygen Species, ROS)는 피부노화에 가장 중요한 원인으로 꼽히며, 주름, 아토피, 피부염 등의 영향을 미친다. 따라서 기존의 화장료 조성물들은 항산화제를 활용하여 자유 라디칼과 병합하거나 중화하여 ROS에 의한 손상을 완화하는데 목적을 두고 있다.Since the skin is the first factor to determine the degree of aging, many people are trying to prevent skin aging in various ways. In particular, Reactive Oxygen Species (ROS) generated through ultraviolet rays, stress, and environmental pollutants is considered the most important cause of skin aging and has an effect on wrinkles, atopy, and dermatitis. Therefore, existing cosmetic compositions aim to alleviate damage caused by ROS by merging or neutralizing free radicals by utilizing antioxidants.
한편, 외부로부터 자극을 받은 피부 세포는 이를 방어하기 위해 높은 에너지를 필요로 한다. 진피의 경우 주로 미토콘드리아에서 산화적 인산화 과정을 통해 에너지를 공급받는 것으로 알려져 있다. 에너지 생성이 감소하면 교원섬유와 탄력섬유의 합성 및 항산화 효소 합성에 필요한 에너지를 충분히 공급받지 못해 교원섬유와 탄력섬유의 발현을 감소시켜 피부 주름을 생성시킬 수 있다.On the other hand, skin cells stimulated from the outside require high energy to defend them. In the case of the dermis, it is known that energy is supplied mainly through oxidative phosphorylation in mitochondria. When energy production is reduced, the energy required for the synthesis of collagen and elastic fibers and the synthesis of antioxidant enzymes is not sufficiently supplied, and the expression of collagen and elastic fibers is reduced, which can cause skin wrinkles.
또한, 미토콘드리아 기능 이상으로 인해 활성산소종이 증가하는 경우, 이들 물질이 멜라닌 생성을 촉진하고, 색소침착을 유발하며 피부 내 단백질, 지방, 세포막, 핵산 등을 산화시켜 피부 노화를 더욱 촉진할 수도 있다.In addition, when reactive oxygen species increase due to abnormal mitochondrial function, these substances promote melanin production, cause pigmentation, and oxidize proteins, fats, cell membranes, nucleic acids, etc. in the skin, thereby further promoting skin aging.
더욱이 미토콘드리아 DNA는 세포의 핵 DNA와는 달리 자체적인 수선 기작(repair mechanism)이 없으며, DNA를 보호하는 역할을 하는 히스톤 단백질이 없기 때문에 상대적으로 손상되기 쉽다. 따라서 노화 및 활성산소로 인해 미토콘드리아 DNA 손상 및 기능 이상이 생기면, 에너지 생성이 감소되고, 동시에 전자 유출이 증가하여 활성 산소를 더 많이 생성하게 된다. 증가한 활성 산소는 다시 미토콘드리아를 표적으로 해 미토콘드리아의 기능 이상을 초래하여 과도한 활성산소 발생으로 이어진다 (Prahl, S., et al., 2008, 32.1-4: 245-255). Moreover, mitochondrial DNA, unlike nuclear DNA of cells, does not have its own repair mechanism and is relatively susceptible to damage because there is no histone protein that protects the DNA. Therefore, when mitochondrial DNA damage and dysfunction occur due to aging and free radicals, energy production is reduced, and at the same time, electron flux is increased to generate more active oxygen. The increased free radicals target the mitochondria again, resulting in mitochondrial dysfunction, leading to excessive generation of reactive oxygen species (Prahl, S., et al., 2008, 32.1-4: 245-255).
이러한 기술적 배경하에서, 미토콘드리아 보호 및 기능 활성화를 통해 미토콘드리아의 에너지 생성을 촉진하는 것이 산화적 스트레스를 저해하고, 피부 노화를 방지할 수 있는 방법으로 제시될 수 있다.Under this technical background, promoting mitochondrial energy generation through mitochondrial protection and functional activation can be suggested as a method to inhibit oxidative stress and prevent skin aging.
이에 본 발명의 목적은 보리밥나무천연 물질을 이용하여 친환경적이면서도, 산화적 스트레스로부터 세포 사멸을 억제하여 피부 세포를 보호하고, 피부 세포 내 미토콘드리아 DNA 합성 증가 및 기능 활성화를 통해 활성산소를 억제함으로써, 피부를 개선하는 조성물을 제공하는 데 있다.Accordingly, an object of the present invention is to protect skin cells by inhibiting apoptosis from oxidative stress while being eco-friendly by using barley natural material, and by inhibiting active oxygen through increased mitochondrial DNA synthesis and functional activation in skin cells. To provide a composition to improve the.
본 발명의 추가적인 목적은 인체에 해가 없는 친환경적인 피부개선용 화장료 조성물을 제공하는 것이다.An additional object of the present invention is to provide an eco-friendly cosmetic composition for skin improvement that is harmless to the human body.
본 발명의 또 다른 목적은 인체에 해가 없는 친환경적인 피부개선용 건강기능성식품 조성물을 제공하는 것이다.Another object of the present invention is to provide an eco-friendly functional health food composition for skin improvement that is harmless to the human body.
상기 목적을 달성하기 위하여, 본 발명은 보리밥나무 추출물을 유효성분으로 포함하는 피부개선용 조성물을 제공한다.In order to achieve the above object, the present invention provides a composition for improving skin comprising a barley rice tree extract as an active ingredient.
본 발명의 일 실시예에 의하면, 상기 피부개선이 항산화 및 항노화인 것이 바람직하다.According to an embodiment of the present invention, it is preferable that the skin improvement is antioxidant and anti-aging.
본 발명의 일 실시예에 따르면, 상기 조성물은 산화스트레스에 대한 피부 세포 보호용인 것이 바람직하다.According to an embodiment of the present invention, the composition is preferably for protecting skin cells against oxidative stress.
본 발명의 일 실시예에 의하면, 상기 조성물은 미토콘드리아의 보호 및 기능 활성화에 의한 피부 개선용인 것이 바람직하다.According to an embodiment of the present invention, the composition is preferably for skin improvement by mitochondrial protection and functional activation.
본 발명의 일 실시예에 의하면, 상기 조성물은 피부 주름 개선용인 것이 바람직하다.According to an embodiment of the present invention, the composition is preferably for improving skin wrinkles.
본 발명의 일 실시예에 의하면, 상기 조성물은 피부 활력 증진용인 것이 바람직하다.According to one embodiment of the present invention, the composition is preferably for promoting skin vitality.
본 발명의 일 실시예에 의하면, 상기 조성물은 활성산소종(ROS)의 감소에 의한 피부 개선용인 것이 바람직하다.According to one embodiment of the present invention, the composition is preferably for skin improvement by reducing reactive oxygen species (ROS).
본 발명의 일 실시예에 의하면, 상기 조성물은 보리밥나무 추출물을 상기 조성물 총 중량에 대하여 0.0001~10% 중량%로 포함하는 것이 바람직하다.According to an embodiment of the present invention, the composition preferably contains the barley rice tree extract in an amount of 0.0001 to 10% by weight based on the total weight of the composition.
본 발명의 일 실시예에 의하면, 상기 보리밥나무 추출물은 물, 유기용매 또는 이들의 혼합 용매로 추출되는 것이 바람직하다.According to an embodiment of the present invention, it is preferable that the extract of barley rice tree is extracted with water, an organic solvent, or a mixed solvent thereof.
본 발명의 일 실시예에 의하면, 상기 유기용매는 메탄올, 에탄올, 프로필알코올, 부틸알코올, 글리세린, 프로필렌글리콜 및 부틸렌글리콜 중에서 선택된 1종 이상인 것이 바람직하다.According to an embodiment of the present invention, the organic solvent is preferably at least one selected from methanol, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol and butylene glycol.
본 발명의 일 실시예에 의하면, 상기 조성물이 화장료 조성물로 사용되는 것이 바람직하다.According to an embodiment of the present invention, the composition is preferably used as a cosmetic composition.
본 발명의 일 실시예에 의하면, 상기 화장료 조성물이 수용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성 함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션 및 스프레이로 구성된 군으로부터 선택되는 어느 하나의 제형인 것이 바람직하다.According to an embodiment of the present invention, the cosmetic composition is an aqueous solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray It is preferable that any one formulation selected from the group consisting of
본 발명의 일 실시예에 의하면, 상기 화장료 조성물은 수중유형 및 유중수형 메이크업베이스, 파운데이션, 스킨커버, 립스틱, 립그로스, 페이스파우더, 투웨이케익, 아이섀도, 치크칼라 및 아이브로우 펜슬류로 이루어진 색조화장료 제형 중에서 선택되는 어느 하나의 제형인 것이 바람직하다.According to one embodiment of the present invention, the cosmetic composition is a color made of oil-in-water and water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color and eyebrow pencils. It is preferable that any one formulation selected from cosmetic formulations.
본 발명의 일 실시예에 의하면, 상기 조성물이 건강기능성식품 조성물인 것이 바람직하다.According to an embodiment of the present invention, it is preferable that the composition is a health functional food composition.
본 발명의 일 실시예에 의하면, 상기 건강기능식품 조성물은 캡슐, 정제, 과립, 분말 또는 음료 형태인 것이 바람직하다. According to an embodiment of the present invention, the health functional food composition is preferably in the form of capsules, tablets, granules, powders or beverages.
본 발명에 따른 조성물은 산화적 스트레스로부터 세포 사멸을 억제하여 세포의 생존율을 증가시켜 세포 보호 효과를 나타내므로, 이에 따라서 피부 탄력, 주름 방지 및 노화 방지 효과를 나타낸다.The composition according to the present invention suppresses apoptosis from oxidative stress to increase cell viability, thereby exhibiting a cytoprotective effect, thus exhibiting skin elasticity, anti-wrinkle and anti-aging effects.
본 발명에 따른 조성물은 산화적 스트레스에 대하여 미토콘드리아 DNA 합성을 증가시키고, 주요 기능인 ATP 생성을 증가시켜 기능을 활성화시켜 미토콘드리아를 보호하고 기능을 활성화시켜 피부를 개선하는 효과가 있다.The composition according to the present invention increases mitochondrial DNA synthesis against oxidative stress and activates the function by increasing ATP production, which is the main function, thereby protecting mitochondria and activating the function to improve the skin.
또한 본 발명에 따른 조성물은 미토콘드리아의 주요 기능인 ATP 생성을 증가시킴으로서 피부 활력을 증가시키는 효과가 있다.In addition, the composition according to the present invention has an effect of increasing skin vitality by increasing ATP production, which is a major function of mitochondria.
또한, 본 발명에 따른 조성물은, ROS 생성량을 감소시켜, 주름, 아토피, 피부염 등의 피부 문제를 개선시키는 효과를 나타낸다.In addition, the composition according to the present invention exhibits an effect of improving skin problems such as wrinkles, atopy, and dermatitis by reducing the amount of ROS production.
본 발명에 따른 조성물은 피부 세포 노화를 방지하는 효과를 나타낸다.The composition according to the present invention exhibits an effect of preventing skin cell aging.
본 발명에 따른 조성물은 피부 세포에 악영향을 미치지 않는 안전성을 가지면서 항산화 효과 및 피부 세포 노화를 방지하므로 피부 외용제, 화장료 조성물 및 약학 조성물에 널리 적용될 수 있다.The composition according to the present invention can be widely applied to external preparations for skin, cosmetic compositions, and pharmaceutical compositions because it has safety that does not adversely affect skin cells and prevents antioxidant effects and skin cell aging.
도 1은 인간 정상 섬유아세포에 보리밥나무의 가지 또는 잎 추출물을 처리하여 세포 독성 여부를 MTT assay를 통해 확인한 결과이다. (대조군으로는 어떠한 처리도 하지 않은 인간 정상 섬유아세포를, 음성대조군으로는 DMSO를 처리한 인간 정상 섬유아세포를 사용하였다.)
도 2는 보리밥나무의 가지 또는 잎 추출물이 처리된 인간 정상 섬유아세포의 과산화수소에 대한 세포보호효과를 MTT assay를 통하여 확인한 결과이다. (대조군으로는 어떠한 처리도 하지 않은 인간 정상 섬유아세포를, 음성대조군으로는 과산화수소만을 처리한 인간 정상 섬유아세포를, 양성대조군으로는 과산화수소와 10μM의 Ascorbic acid를 처리한 인간 정상 섬유아세포를 사용하였다.)
도 3은 보리밥나무의 가지 또는 잎 추출물이 처리된 인간 정상 섬유아세포의 미토콘드리아 DNA Copy 수를 real time PCR를 통하여 측정한 결과이다. (대조군으로는 어떠한 처리도 하지 않은 인간 정상 섬유아세포를, 음성대조군으로는 과산화수소만을 처리한 인간 정상 섬유아세포를, 양성대조군으로는 과산화수소와 10μM의 Ascorbic acid를 처리한 인간 정상 섬유아세포를 사용하였다.)
도 4는 보리밥나무의 가지 또는 잎 추출물이 처리된 인간 정상 섬유아세포의 미토콘드리아 ATP 합성을 측정한 결과이다. (대조군으로는 어떠한 처리도 하지 않은 인간 정상 섬유아세포를, 음성대조군으로는 과산화수소만을 처리한 인간 정상 섬유아세포를, 양성대조군으로는 과산화수소와 10μM의 Ascorbic acid를 처리한 인간 정상 섬유아세포를 사용하였다.)
도 5는 보리밥나무의 가지 또는 잎 추출물이 처리된 인간 정상 섬유아세포의 ROS 생성량을 DCF-DA를 통해 측정한 결과이다. (대조군으로는 어떠한 처리도 하지 않은 인간 정상 섬유아세포를, 음성대조군으로는 과산화수소만을 처리한 인간 정상 섬유아세포를, 양성대조군으로는 과산화수소와 10μM의 Ascorbic acid를 처리한 인간 정상 섬유아세포를 사용하였다.)1 is a result of confirming whether or not cytotoxicity through MTT assay by treating normal human fibroblasts with branch or leaf extracts of barley. (As a control group, normal human fibroblasts without any treatment were used, and as a negative control group, normal human fibroblasts treated with DMSO were used.)
2 is the result of confirming the cytoprotective effect on hydrogen peroxide in normal human fibroblasts treated with branch or leaf extracts of barley tree through MTT assay. (As a control group, normal human fibroblasts without any treatment were used, as a negative control, human normal fibroblasts treated only with hydrogen peroxide, and as a positive control, normal human fibroblasts treated with hydrogen peroxide and 10 μM ascorbic acid were used. )
3 is a result of measuring the number of mitochondrial DNA copies of normal human fibroblasts treated with branch or leaf extracts of barley tree through real-time PCR. (As a control group, normal human fibroblasts without any treatment were used, as a negative control, human normal fibroblasts treated only with hydrogen peroxide, and as a positive control, normal human fibroblasts treated with hydrogen peroxide and 10 μM ascorbic acid were used. )
4 is a result of measuring mitochondrial ATP synthesis in normal human fibroblasts treated with branch or leaf extracts of barley. (As a control group, normal human fibroblasts without any treatment were used, as a negative control, human normal fibroblasts treated only with hydrogen peroxide, and as a positive control, normal human fibroblasts treated with hydrogen peroxide and 10 μM ascorbic acid were used. )
5 is a result of measuring the ROS production amount of normal human fibroblasts treated with a branch or leaf extract of barley tree through DCF-DA. (As a control group, normal human fibroblasts without any treatment were used, as a negative control, human normal fibroblasts treated only with hydrogen peroxide, and as a positive control, normal human fibroblasts treated with hydrogen peroxide and 10 μM ascorbic acid were used. )
이하, 본 발명의 내용을 하기 실시예를 통해 상세히 설명하고자 한다. 이들 실시 예는 오로지 본 발명을 예시하기 위한 것일 뿐, 본 발명의 범위가 이들 실시 예에 의해 한정되는 것은 아니다.Hereinafter, the contents of the present invention will be described in detail through the following examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.
본 발명에 따른 조성물은 보리밥나무 추출물을 유효성분으로 포함하는 피부개선용 조성물이다.The composition according to the present invention is a composition for improving skin comprising a barley extract as an active ingredient.
본 발명에 있어서 용어 "피부 개선용"이란, 피부 보호 및 피부 상태 개선, 피부 미백, 피부 노화 및 주름의 예방 또는 개선, 피부 보습, 피지분비억제, 여드름개선, 모공축소, 피부보호 및 피부의 염증 반응의 완화, 항염, 피부 장벽 기능, 보습 개선, 피부 활력 증진, 자외선으로부터의 피부 보호, 항산화 및 항노화 등을 모두 포함하는 개념이다. In the present invention, the term "for skin improvement" means skin protection and skin condition improvement, skin whitening, prevention or improvement of skin aging and wrinkles, skin moisturizing, sebum secretion suppression, acne improvement, pore reduction, skin protection and inflammation of the skin It is a concept that includes alleviation of reaction, anti-inflammatory, skin barrier function, moisture improvement, skin vitality enhancement, skin protection from UV rays, antioxidant and anti-aging.
본 발명에서 "피부"는 얼굴 뿐만 아니라, 두피, 전신도 포함되는 개념이다.In the present invention, "skin" is a concept that includes not only the face, but also the scalp and the whole body.
여기서 "유효성분으로 함유하는"은 피부 개선 효과를 나타낼 수 있는 정도의 보리밥나무 추출물을 포함한다는 것을 의미한다.Here, "contained as an active ingredient" means to include a barley extract to an extent capable of exhibiting a skin improvement effect.
보리밥나무(Elaeaqnus macrophylla_는 보리수나무과 보릿수나무속에 속하는 상록 관목 또는 덩굴나무로서 한국, 중국, 일본, 타이완에 분포한다. 사람 키 높이 정도로 자라며 약간 덩굴진다. 꽃은 10-11월에 잎겨드랑이에서 백색 또는 연한 황백색의 양성화가 1-3개씩 모여 달린다.Barley rice tree (Elaeaqnus macrophylla_) is an evergreen shrub or vine belonging to the genus Bodice and is distributed in Korea, China, Japan, and Taiwan. It grows to the height of a person and has vines slightly. The flowers are in the leaf axil in October-November. White or light yellowish-white bisexual flowers run in groups of 1-3.
상기 보리밥나무는 잎, 꽃, 줄기, 뿌리 모두 다 사용가능하며, 바람직하게는 가지, 잎이 사용될 수 있고, 특히 건조한 후 사용하는 것이 바람직하다. All of the leaves, flowers, stems, and roots of the barley rice tree can be used, preferably branches and leaves can be used, and in particular, it is preferable to use them after drying.
본 발명의 일 실시예에 의하면 상기 피부개선은 항산화 및 항노화인 것이 바람직하다.According to an embodiment of the present invention, it is preferable that the skin improvement is antioxidant and anti-aging.
본 발명에서 용어 "항산화"란 대사 과정을 통해 생성된 활성 산소가 축적되면 조직 세포의 노화, 퇴행성 질환 등이 유발되는데 이러한 활성 산소의 생성을 억제하는 것을 말한다.In the present invention, the term "antioxidant" refers to inhibiting the generation of active oxygen, which is caused by the accumulation of active oxygen produced through metabolic processes, such as aging of tissue cells, degenerative diseases, and the like.
본 발명에서 용어 "노화"란 피부 내 또는 피부 외 요인으로 인하여 세포 기능이 저하되어 피부 주름 발생, 피부 탄력 저하, 피부 건조, 색소 침착, 또는 피부 재생력 저하 등을 말하며, "항노화"란 노화를 방지하는 것을 말한다.In the present invention, the term "aging" refers to the occurrence of skin wrinkles, decreased skin elasticity, dry skin, pigmentation, or decreased skin regeneration ability due to a decrease in cell function due to factors inside or outside the skin, and "anti-aging" refers to aging say to prevent
본 발명의 일 실시예에 의하면, 상기 조성물은 산화스트레스에 대한 피부 세포 보호용인 것이 바람직하다.According to an embodiment of the present invention, the composition is preferably for protecting skin cells against oxidative stress.
본 발명에서 용어 "산화스트레스"란 체내 활성산소가 많아져 생체 산화 균형이 무너진 상태를 말한다.As used herein, the term "oxidative stress" refers to a state in which the body's oxidative balance is disrupted due to an increase in free radicals in the body.
본 발명의 일 실시예에 의하면, 상기 조성물은 미토콘드리아의 보호 및 기능 활성화에 의한 피부 개선용인 것이 바람직하다.According to an embodiment of the present invention, the composition is preferably for skin improvement by mitochondrial protection and functional activation.
본 발명에서 용어 "미토콘드리아의 보호 및 기능 활성화"란 미토콘드리아의 DNA의 합성 및/또는 미토콘드리아의 ATP 합성을 통해 미토콘드리아를 보호하고 그 기능을 지속적으로 유지 및 활성화시키는 것을 말한다.In the present invention, the term "protection and function activation of mitochondria" refers to protecting mitochondria through the synthesis of mitochondrial DNA and/or mitochondrial ATP synthesis and continuously maintaining and activating its functions.
본 발명의 일 실시예에 의하면, 상기 조성물은 피부 주름 개선용인 것이 바람직하다.According to an embodiment of the present invention, the composition is preferably for improving skin wrinkles.
본 발명의 일 실시예에 의하면, 상기 조성물은 피부 활력 증진용인 것이 바람직하다.According to one embodiment of the present invention, the composition is preferably for promoting skin vitality.
본 발명의 일 실시예에 의하면, 상기 조성물은 활성산소종(ROS)의 감소에 의한 피부 개선용인 것이 바람직하다.According to one embodiment of the present invention, the composition is preferably for skin improvement by reducing reactive oxygen species (ROS).
본 발명에서 용어 "활성산소종(ROS)"란 화학적으로 활성화된, 즉 불안정한 산소를 포함하는 화학물질들을 말하며, 일반적으로 산소 대비 화학적 반응성이 높은 산소를 포함하는 분자들의 총칭으로, 생체 조직을 공격하고 세포를 손상시키는 산화력이 강한 산소이다.In the present invention, the term "reactive oxygen species (ROS)" refers to chemically activated, that is, chemical substances containing unstable oxygen, and is a generic term for molecules containing oxygen with high chemical reactivity compared to oxygen. It is oxygen with strong oxidizing power that damages cells.
본 발명의 일 실시예에 의하면, 상기 조성물은 보리밥나무 추출물을 상기 조성물 총 중량에 대하여 0.0001~10% 중량% 로 포함하는 것이 바람직하다.According to an embodiment of the present invention, the composition preferably contains the barley rice tree extract in an amount of 0.0001 to 10% by weight based on the total weight of the composition.
보리밥나무 추출물이 0.0001중량% 미만으로 포함되면, 항산화 및 항노화 효능의 발휘가 나타나기 어려울 수 있으며, 10중량% 이상 포함되는 경우 변색, 변취 등 제형의 안정성이 저하되어 항산화 및 항노화 효과가 미미해질 수 있으며, 화장료 등으로 제조 시 향이 좋지 않을 수 있다.When the barley extract is included in an amount of less than 0.0001% by weight, it may be difficult to exhibit antioxidant and anti-aging effects. Also, the fragrance may not be good when manufactured with cosmetics.
본 발명의 일 실시예에 의하면, 본 발명에서 추출 방법은 특별히 제한되지 않고, 예를 들어 냉침 추출, 초음파 추출, 환류 추출, 열수 추출 등이 있다. 여기서, 열수추출의 경우, 열탕증류기에서 8~48시간동안, 80~ 100℃ 로 가열하여 열수 추출물을 얻는다. 냉수 추출의 경우, 예컨대, 냉수 (15-25℃)에 상기 태좌조직 배양물 자체 또는 그것의 건조된 분말을 혼합하여 3일동안 추출하여 냉수추출물을 얻는다.According to an embodiment of the present invention, the extraction method in the present invention is not particularly limited, and for example, cold extraction, ultrasonic extraction, reflux extraction, hot water extraction, and the like. Here, in the case of hot water extraction, the hot water extract is obtained by heating at 80 to 100° C. for 8 to 48 hours in a hot water distiller. In the case of cold water extraction, for example, the placental tissue culture itself or its dried powder is mixed with cold water (15-25° C.) and extracted for 3 days to obtain a cold water extract.
또는, 물, 유기 용매, 또는 이의 혼합 용매를 사용하여 추출하는 방법으로 제조될 수 있다. 추출한 액은 바로 사용하거나 또는 농축 및/또는 건조하여 사용할 수 있다. 유기용매를 사용하여 추출하는 경우, 메탄올, 에탄올, 이소프로판올, 부탄올, 에틸렌, 아세톤, 헥산, 에테르, 클로로포름, 에틸아세테이트, 부틸아세테이트, 디클로로메탄, N, N-디메틸포름아미드(DMF), 디메틸설폭사이드(DMSO), 1,3-부틸렌글리콜, 프로필렌글리콜 또는 이들의 혼합용매인 유기용매를 사용하며 생약의 유효 성분이 파괴되지 않거나 최소화된 조건에서 실온 또는 가온하여 추출할 수 있다. Alternatively, it may be prepared by extraction using water, an organic solvent, or a mixed solvent thereof. The extracted liquid may be used directly or concentrated and/or dried. In case of extraction using an organic solvent, methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixture thereof, an organic solvent, is used, and the active ingredient of the herbal medicine is not destroyed or can be extracted by heating at room temperature or under conditions that are minimized.
본 발명의 일 실시예에서, 상기 보리밥나무 추출물은 물, 유기용매 또는 이들의 혼합 용매로 추출되는 것이 바람직하다.In one embodiment of the present invention, it is preferable that the extract of barley rice tree is extracted with water, an organic solvent, or a mixed solvent thereof.
특히, 본 발명에 있어서는 에탄올 추출물, 바람직하게는 20-80% 에탄올 추출물, 일 구체예로써 80% 에탄올 추출물이 바람직하며, 추출 방법은 80% 에탄올에 섞은 후 3일동안 교반하여 얻는다. 본 발명에 있어서, 상기 추출물은 농축, 또는 희석하여 사용할 수 있고, 추출물의 증류액을 사용할 수도 있다.In particular, in the present invention, an ethanol extract, preferably a 20-80% ethanol extract, as an embodiment, an 80% ethanol extract is preferable, and the extraction method is obtained by mixing with 80% ethanol and stirring for 3 days. In the present invention, the extract may be used after concentration or dilution, or a distillate of the extract may be used.
본 발명의 일 실시예에 의하면, 상기 유기용매는 메탄올, 에탄올, 프로필알코올, 부틸알코올, 글리세린, 프로필렌글리콜 및 부틸렌글리콜 중에서 선택된 1종 이상인 것이 바람직하다.According to an embodiment of the present invention, the organic solvent is preferably at least one selected from methanol, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol and butylene glycol.
본 발명의 일 실시예에 의하면, 상기 조성물이 화장료 조성물로 사용되는 것이 바람직하다.According to an embodiment of the present invention, the composition is preferably used as a cosmetic composition.
본 발명의 일 실시예에 의하면, 상기 화장료 조성물이 수용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성 함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션 및 스프레이로 구성된 군으로부터 선택되는 어느 하나의 제형인 것이 바람직하다.According to an embodiment of the present invention, the cosmetic composition is an aqueous solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray It is preferable that any one formulation selected from the group consisting of
본 발명의 일 실시예에 의하면, 상기 화장료 조성물은 수중유형 및 유중수형 메이크업베이스, 파운데이션, 스킨커버, 립스틱, 립그로스, 페이스파우더, 투웨이케익, 아이섀도, 치크칼라 및 아이브로우 펜슬류로 이루어진 색조화장료 제형 중에서 선택되는 어느 하나의 제형인 것이 바람직하다.According to one embodiment of the present invention, the cosmetic composition is a color made of oil-in-water and water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color and eyebrow pencils. It is preferable that any one formulation selected from cosmetic formulations.
본 발명의 일 실시예에 의하면, 상기 조성물이 건강기능성식품 조성물인 것이 바람직하다.According to an embodiment of the present invention, it is preferable that the composition is a health functional food composition.
본 발명의 일 실시예에 의하면, 상기 건강기능식품 조성물은 캡슐, 정제, 과립, 분말 또는 음료 형태인 것이 바람직하다. According to an embodiment of the present invention, the health functional food composition is preferably in the form of capsules, tablets, granules, powders or beverages.
상기 화장료 조성물에 있어서는, 화장품 제제에 있어서 수용가능한 담체를 포함할 수 있다. 여기서, "화장품 제제에 있어서 수용가능한 담체"란 화장품 제제에 포함될 수 있는 이미 공지되어 사용되고 있는 화합물 또는 조성물이거나 앞으로 개발될 화합물 또는 조성물로서 피부와의 접촉시 인체가 적응 가능한 이상의 독성, 불안정성 또는 자극성이 없는 것을 말한다.In the cosmetic composition, it may include a carrier acceptable in the cosmetic formulation. Herein, "acceptable carrier for cosmetic preparations" refers to compounds or compositions that are already known and used that can be included in cosmetic preparations, or compounds or compositions to be developed in the future that have toxicity, instability, or irritation beyond what the human body can adapt when in contact with the skin. say nothing
싱기 담체는 본 발명의 화장료 조성물에 그것의 전체 중량에 대하여 약 1 중량 % 내지 약 99.99 중량 %, 바람직하게는 조성물의 중량의 약 90 중량% 내지 약 99.99 중량 %로 포함될 수 있다. 그러나 상기 비율은 본 발명의 화장료 조성물이 제조되는 후술한 바의 제형에 따라 또 그것의 구체적인 적용 부위(얼굴, 목 등)나 그것의 바람직한 적용량 등에 따라 달라지는 것이기 때문에, 상기 비율은 어떠한 측면으로든 본 발명의 범위를 제한하는 것으로 이해되어서는 안 된다.The Shingi carrier may be included in the cosmetic composition of the present invention in an amount of from about 1% to about 99.99% by weight, preferably from about 90% to about 99.99% by weight of the total weight of the composition. However, since the ratio varies depending on the formulation as described below in which the cosmetic composition of the present invention is prepared, its specific application site (face, neck, etc.) or its desired application amount, the ratio is should not be construed as limiting the scope of
상기 담체로서는 알코올, 오일, 계면활성제, 지방산, 실리콘 오일, 습윤제, 보습제, 점성 변형제, 유제, 안정제, 자외선산란제, 자외선흡수제, 발색제, 향료 등이 예시될 수 있다. 상기 알코올, 오일, 계면활성제, 지방산, 실리콘 오일, 습윤제, 보습제, 점성 변형제, 유제, 안정제, 자외선산란제, 자외선흡수제, 발색제, 향료로 사용될 수 있는 화합물/조성물 등은 이미 당업계에 공지되어 있기 때문에 당업자라면 적절한 해당 물질/조성물을 선택하여 사용할 수 있다.Examples of the carrier include alcohol, oil, surfactant, fatty acid, silicone oil, humectant, humectant, viscosity modifier, emulsion, stabilizer, UV scattering agent, UV absorber, color developer, fragrance, and the like. The alcohol, oil, surfactant, fatty acid, silicone oil, wetting agent, humectant, viscosity modifier, emulsion, stabilizer, UV scattering agent, UV absorber, coloring agent, perfume, etc. are already known in the art. Therefore, those skilled in the art can select and use an appropriate material/composition.
본 발명의 일 구현예로써, 본 발명에 따른 화장료 조성물은 상기 유효성분 이외에 글리세린, 부틸렌글리콜, 프로필렌글키롤, 폴리옥시에틸렌 경화피마자유, 에탄올, 트리에탄올아민 등을 포함할 수 있으며, 방부제, 항료, 착색료, 정제수 등을 필요에 따라 미량 포함할 수 있다.As an embodiment of the present invention, the cosmetic composition according to the present invention may include glycerin, butylene glycol, propylene glycol, polyoxyethylene hydrogenated castor oil, ethanol, triethanolamine, etc. in addition to the active ingredients, and preservatives, fragrances , a colorant, purified water, etc. may be included in a trace amount as needed.
또한, 종래에 잘 알려져 있는 생리활성성분이나 다른 태좌세포 배양물 또는 추출물을 더 포함할 수 있다.In addition, it may further include physiologically active ingredients or other placental cell cultures or extracts known in the prior art.
본 발명에 있어서의 피부는 얼굴 뿐만 아니라, 두피, 전신도 포함되는 개념으로, 이러한 두피에 적용될 수 있는 화장료 조성물로써, 샴푸, 린스, 트리트먼트, 발모제 등이 있고, 전신에 적용될 수 있는 바디클렌져 등의 용도로써 다양한 형태로 제조될 수 있다.The skin in the present invention is a concept that includes not only the face, but also the scalp and the whole body. As a cosmetic composition that can be applied to the scalp, there are shampoos, conditioners, treatments, hair growth agents, etc., and body cleansers that can be applied to the whole body, etc. It can be prepared in various forms for the purpose of
본 발명에 따른 화장료 조성물의 제조방법은 전술한 제조방법에 한정되는 것은 아니며, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자라면 상기 제조방법을 일부 변형시킨 방법으로도 제조할 수 있다.The manufacturing method of the cosmetic composition according to the present invention is not limited to the above-described manufacturing method, and those skilled in the art to which the present invention pertains may also prepare a method by partially modifying the above manufacturing method.
또한, 본 발명의 화장료 조성물은 추가로 지방 물질, 유기 용매, 용해제, 농축제 및 겔화제, 연화제, 항산화제, 현탁화제, 안정화제, 발포제(foaming agent), 방향제, 계면활성제, 물, 이온형 또는 비이온형 유화제, 충전제, 금속이온봉쇄제 및 킬레이트화제, 보존제, 비타민, 차단제, 습윤화제, 필수 오일,염료,안료, 친수성 또는 친유성 활성제, 지질 소낭 또는 화장품에 통상적으로 사용되는 임의의 다른 성분과 같은 화장품학 또는 피부과학 분야에서 통상적으로 사용되는 보조제를 함유할 수 있다. 그리고, 상기의 성분들은 피부과학 분야에서 일반적으로 사용되는 양으로 도입될 수 있다.In addition, the cosmetic composition of the present invention is a fatty substance, an organic solvent, a solubilizer, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic type. or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other conventionally used in cosmetics. It may contain adjuvants commonly used in the field of cosmetology or dermatology, such as ingredients. And, the above ingredients may be introduced in an amount generally used in the field of dermatology.
본 발명의 또 다른 실시예에 따라 본 발명의 건강음료 조성물은 지시된 비율로 필수성분으로서 상기 분획물을 함유하는 외에는 액체성분에는 특별한 제한점은 없으며, 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다. 상기 천연 탄수화물의 예는 모노사카라이드, 예를 들어, 포도당, 과당 등; 디사카라이드, 예를 들어 말토스, 슈크로스 등; 및 폴리사카라이드, 예를 들어 덱스트린, 시클로덱스트린 등과 같은 통상적인 당 및 자일리톨, 소르비톨, 에리스리톨 등의 당알콜이다. 상술한 것 이외의 향미제로서 천연 향미제(타우마틴, 스테비아 추출물(예를 들어 레바우디오시드 A, 글리시르히진 등) 및 합성 향미제(사카린, 아스파탐 등)를 유리하게 사용할 수 있다. 또한, 상기 천연 탄수화물의 비율은 본 발명의 조성물 100 ㎖ 당 일반적으로 약 1 ~ 20 g, 또는 약 5 ~ 12 g이다.According to another embodiment of the present invention, the health beverage composition of the present invention has no particular limitation on the liquid component except for containing the fraction as an essential component in the indicated ratio, and various flavoring agents or natural carbohydrates, etc. may be contained as an additional component. Examples of the natural carbohydrate include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. , the proportion of the natural carbohydrate is generally about 1 to 20 g, or about 5 to 12 g per 100 ml of the composition of the present invention.
상기 외에 본 발명의 조성물은 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 증진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산 음료에 사용되는 탄산화제 등을 함유할 수 있다.In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavoring agents, coloring agents and enhancers (cheese, chocolate, etc.), pectic acid and salts thereof, alginic acid and salts thereof , organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like.
그밖에 본 발명의 조성물들은 천연 과일 쥬스 및 과일 쥬스 음료 및 야채 음료의 제조를 위한 과육을 함유할 수 있다. 이러한 성분은 독립적으로 또는 조합하여 사용할 수 있다. 이러한 첨가제의 비율은 본 발명의 조성물 100중량부당 0 ~ 약 20 중량부의 범위에서 선택되는 것이 일반적이다.In addition, the compositions of the present invention may contain natural fruit juice and pulp for the production of fruit juice beverages and vegetable beverages. These components may be used independently or in combination. The proportion of these additives is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.
이하 본 발명을 하기 실시예에 따라 상세히 설명한다. 단 하기 실시예는 본 발명을 보다 상세히 설명하기 위한 것일 뿐이며, 본 발명의 범위를 제한하는 방법으로 해석되어서는 안된다. Hereinafter, the present invention will be described in detail according to the following examples. However, the following examples are only for illustrating the present invention in more detail, and should not be construed as limiting the scope of the present invention.
제조예 1: 보리밥나무 추출물의 제조Preparation Example 1: Preparation of barley extract
건조된 보리밥나무를 분쇄하여 분말화 한 후, 2 배량의 80% 에탄올에 24시간 이상 침지하여 유효성분을 추출한다. 이렇게 추출된 추출물을 여과지에 거른 다음 회전감압농축기로 용매를 제거하여 추출물을 제조하였다.After pulverizing and powdering the dried barley, the active ingredient is extracted by immersing it in twice the amount of 80% ethanol for more than 24 hours. The extract thus extracted was filtered through a filter paper and the solvent was removed with a rotary vacuum concentrator to prepare an extract.
[시험예 1: 보리밥나무 추출물의 세포 독성 평가][Test Example 1: Evaluation of cytotoxicity of barley extract]
본 시험예에서는 보리밥나무 추출물의 세포 독성 여부를 확인하고자 농도가 다른 보리밥나무의 가지 또는 잎 추출물을 인간 정상 섬유아세포인 CCD986sk에 처리하여 세포생존능력을 측정하였다.In this test example, cell viability was measured by treatment with CCD986sk, a normal human fibroblast, with extracts of branches or leaves of different concentrations in order to check whether the extracts were cytotoxic.
인간 정상 피부 섬유아세포(CCD-986sk)를 1X104 cells/well 농도로 96-well plate에 분주한 후 37℃, 5% CO2 조건에서 24시간 동안 배양하였다. 배양 배지를 모두 제거 후, 무혈청배지에 실시예 1에 따라 제조된 보리밥나무 가지, 잎 추출물을 각각 0, 10, 50, 100, 200 μg/mL로 희석된 시료를 각 웰 당 100 μL씩 세포에 처리하여 24시간 동안 추가 배양하였다. 그 후, 각 well 당 0.5 mg/ml 농도의 MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) 용액 100 ㎕를 처리하여 3시간동안 반응시킨 후 생성된 포마잔(formazan)을 DMSO 200 ㎕로 용해시켜 micro-plate reader를 이용하여 540 nm 파장에서 흡광도를 측정하였다. 아무것도 처리하지 않은 well의 세포 생존율을 100%으로 하여 정상 대조군 대비 시료 처리군의 세포 생존율을 계산하여 결과를 도 1에 나타내었다.Normal human skin fibroblasts (CCD-986sk) were aliquoted in a 96-well plate at a concentration of 1X10 4 cells/well, and then cultured at 37° C. and 5% CO 2 conditions for 24 hours. After removing all of the culture medium, 100 μL of each well of a sample diluted with 0, 10, 50, 100, and 200 μg/mL of the barley branch and leaf extract prepared according to Example 1 in a serum-free medium, respectively, was applied to cells. was treated and further cultured for 24 hours. After that, 100 μl of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) solution at a concentration of 0.5 mg/ml per well was treated and reacted for 3 hours. The formazan was dissolved in 200 μl of DMSO and absorbance was measured at 540 nm wavelength using a micro-plate reader. The cell viability of the untreated well was calculated as 100%, and the cell viability of the sample treated group compared to the normal control group was calculated, and the results are shown in FIG. 1 .
실험결과, 보리밥나무 추출물을 처리한 인간정상섬유아세포 중, 보리밥나무 가지 추출물 100 μg/mL, 보리밥나무 잎 추출물 100 μg/mL에서 각각 유의적인 세포독성효과가 확인되지 않았다 (도 1). 따라서 100 μg/mL 농도의 보리밥나무 가지, 잎 추출물을 선택하여 하기 실험에 사용하였다. As a result of the experiment, among normal human fibroblasts treated with the barley extract, no significant cytotoxic effect was observed in 100 μg/mL of the barley branch extract and 100 μg/mL of the barley leaf extract ( FIG. 1 ). Therefore, 100 μg/mL of barley branch and leaf extracts were selected and used in the following experiment.
[시험예 2: 보리밥나무 추출물의 세포 보호 효과 확인][Test Example 2: Confirmation of cytoprotective effect of barley extract]
본 시험예에서는 보리밥나무 추출물의 산화 스트레스에 의한 세포 손상 보호 효과를 MTT법을 변형하여 측정하였다. 인간 정상 피부 섬유아세포(CCD-986sk)를 1X104 cells/well 농도로 96-well plate에 분주한 후 37℃, 5% CO2 조건에서 24시간 동안 배양하였다. 배양 배지를 제거 후 무혈청배지에 실시예 1에 따라 제조한 추출물을 각각 희석한 보리밥나무의 가지 또는 잎 추출물을 각각 100 μg/mL 농도의 시료를 세포에 처리하여 24시간 동안 추가 배양하였다. 배양이 끝난 후, 세포 내 산화스트레스를 유발하기 위하여 800 μM의 과산화수소 (hydrogen peroxide, H2O2)를 3시간 동안 처리하였다. 3시간 후, 각 well 당 0.5 mg/ml 농도의 MTT용액 100 ㎕를 처리하여 3시간동안 반응시킨 후 생성된 포마잔(formazan)을 DMSO 100 ㎕로 용해시켜 micro-plate reader를 이용하여 540 nm 파장에서 흡광도를 측정하였다. 정상 대조군으로는 시료와 과산화수소를 처리하지 않은 well을 사용하여 정상 대조군의 세포 생존율을 100%으로 하여 정상 대조군 대비 과산화수소만 처리군 (음성대조군)과 시료 및 과산화수소 처리군, Ascorbic acid 10 μM 처리군의 세포 생존율을 계산하여 결과를 도 2에 나타내었다.In this test example, the protective effect of cellular damage caused by oxidative stress of the barley extract was measured by modifying the MTT method. Normal human skin fibroblasts (CCD-986sk) were aliquoted in a 96-well plate at a concentration of 1X10 4 cells/well, and then cultured at 37° C. and 5% CO 2 conditions for 24 hours. After removing the culture medium, cells were treated with a sample of 100 μg/mL concentration of each of the branch or leaf extracts of barley, each diluted with the extract prepared according to Example 1 in a serum-free medium, and further cultured for 24 hours. After incubation, 800 μM of hydrogen peroxide (H 2 O 2 ) was treated for 3 hours to induce intracellular oxidative stress. After 3 hours, 100 μl of MTT solution of 0.5 mg/ml concentration per well was treated and reacted for 3 hours, then the generated formazan was dissolved with 100 μl of DMSO using a micro-plate reader at a wavelength of 540 nm. absorbance was measured. As a normal control, using wells not treated with sample and hydrogen peroxide, the cell viability of the normal control was set to 100%, and compared to the normal control group, only hydrogen peroxide treatment group (negative control group), sample and hydrogen peroxide treatment group, and ascorbic acid 10 μM treatment group Cell viability was calculated and the results are shown in FIG. 2 .
실험결과, 음성대조군의 경우, 과산화수소 (H2O2)에 의한 산화스트레스 증가로 세포 생존율이 64.5%로 감소하였으나, Ascorbic acid 10 μM을 비롯한 보리밥나무 가지, 잎 추출물을 처리한 인간정상섬유아세포의 경우, 과산화수소 (H2O2)에 의해 감소한 세포 생존률이 회복되어 세포 보호 효과를 나타내었다. 도 2는 보리밥나무 가지 추출물, 보리밥나무 잎 추출물이 100 μg/mL 농도로 처리된 인간정상섬유아세포의 세포보호 효과를 확인한 결과이다.As a result of the experiment, in the case of the negative control group, the cell viability decreased to 64.5% due to the increase in oxidative stress caused by hydrogen peroxide (H 2 O 2 ). In this case, the cell viability decreased by hydrogen peroxide (H 2 O 2 ) was restored, thereby exhibiting a cytoprotective effect. FIG. 2 is a result confirming the cytoprotective effect of normal human fibroblasts treated with a barley tree branch extract and barley leaf extract at a concentration of 100 μg/mL.
[시험예 3: 보리밥나무 추출물이 미토콘드리아의 DNA 합성에 미치는 영향 확인][Test Example 3: Confirmation of effect of barley extract on mitochondrial DNA synthesis]
본 시험예에서는 보리밥나무 추출물이 미토콘드리아의 DNA 합성에 어떠한 영향을 미치는지 확인하고자 하였다. 인간 정상 피부 섬유아세포(CCD-986sk)를 1X105 cells/well 농도로 6-well plate에 분주한 후 37℃, 5% CO2 조건에서 24시간 동안 배양하였다. 배양 배지를 제거 후 무혈청배지에 실시예 1에 따라 제조한 추출물을 각각 희석한 보리밥나무의 가지 또는 잎 추출물 100 μg/mL 농도의 시료를 세포에 처리하여 24시간 동안 추가 배양하였다. 배양이 끝난 후, 세포 내 산화스트레스를 유발하기 위하여 100 μM의 과산화수소 (hydrogen peroxide, H2O2)를 1시간 동안 처리하였다. 1시간 후, 1
세포 내 미토콘드리아 수는 미토콘드리아에서 합성되는 COX Ⅱ (Cytochrome oxidase subunit Ⅱ) 양을 핵에서 만들어지는 cyclophilin A의 양으로 나누어 미토콘드리아 DNA 카피 수를 계산함으로써 측정하였다. 정상 대조군으로는 시료와 과산화수소를 처리하지 않은 미토콘드리아 DNA 카피 수를 1로 하여 정상 대조군 대비 과산화수소만 처리군 (음성대조군)과 시료 및 과산화수소 처리군의 미토콘드리아 DNA Copy 수를 계산하여 결과를 도 3에 나타내었다. The number of mitochondria in cells was measured by dividing the amount of cytochrome oxidase subunit II (COX II) synthesized in mitochondria by the amount of cyclophilin A produced in the nucleus to calculate the mitochondrial DNA copy number. As a normal control, the mitochondrial DNA copy number of the sample and the non-hydrogen peroxide-treated group was set to 1, and the number of mitochondrial DNA copies of the hydrogen peroxide-treated group (negative control group) and the sample and the hydrogen peroxide-treated group compared to the normal control group were calculated and the results are shown in FIG. It was.
실험 결과, 미토콘드리아 DNA(mtDNA)의 카피 수가 과산화수소를 처리한 음성대조군의 경우 정상 대조군 대비 0.49-fold 로 감소하였다. 또한, Ascorbic acid 10 μM을 처리한 실험군의 경우, 정상 대조군 대비 0.62-fold로 음성대조군과 비슷한 수준으로 감소하였다. 보리밥나무 가지, 잎 추출물 100 μg/mL 농도의 시료를 각각 처리한 경우, 미토콘드리아 DNA(mtDNA) 카피 수는 정상 대조군 대비과 동일한 수준으로 증가하였다. 미토콘드리아 DNA(mtDNA) 카피 수의 증가는 미토콘드리아 생성이 증가되었음을 직접적으로 보여주는 지표로 10 μM의 Ascorbic acid는 미토콘드리아 DNA 합성을 유의미하게 증가시키지 못하였으나 보리밥나무 가지, 잎 추출물의 경우, 미토콘드리아의 생성을 증가시킨다는 것을 확인하였다.As a result of the experiment, the copy number of mitochondrial DNA (mtDNA) was reduced to 0.49-fold in the case of the negative control group treated with hydrogen peroxide compared to the normal control group. In addition, in the case of the experimental group treated with 10 μM of ascorbic acid, it was reduced to a level similar to that of the negative control group by 0.62-fold compared to the normal control group. When each sample of barley branch and leaf extract at a concentration of 100 μg/mL was treated, the mitochondrial DNA (mtDNA) copy number was increased to the same level as that of the normal control group. The increase in mitochondrial DNA (mtDNA) copy number is a direct indicator of increased mitochondrial production. Ascorbic acid at 10 μM did not significantly increase mitochondrial DNA synthesis. confirmed that it does.
[시험예 4: 보리밥나무 추출물이 미토콘드리아의 ATP 합성에 미치는 영향 확인][Test Example 4: Confirmation of effect of barley extract on mitochondrial ATP synthesis]
본 시험예에서는 미토콘드리아의 주 기능은 ATP합성으로, 보리밥나무 추출물이 미토콘드리아 내의 ATP 합성에 미치는 영향을 확인하고자 하였다. 사람의 정상섬유아세포인 CCD-986sk 세포를 12-웰 플레이트에 5X104 cells/well의 농도로 접종하고, 37℃, 5% CO2 조건에서 24시간 동안 배양하였다. 그 후, 배양 배지를 제거하고, 혈청이 포함되지 않은 배지에 실시예 1에 따라 제조된 보리밥나무 가지, 잎 추출물 각각을 100 μg/mL 의 농도로 희석된 시료를 well 당 1 ml씩 처리하여 24시간 동안 추가 배양하였다. 그 후, 100 μM의 과산화수소를 1시간 동안 처리한 세포를 PBS로 세척하고, 100 ℃의 증류수를 가해 여러 번 섞어주어 세포를 수거하였다. 수거한 세포를 4 ℃에서 5분간 12,000 xg로 원심분리하여 얻은 상층액 10 ㎕와 미리 준비한 ATP 어세이 혼합물 90 ㎕ 넣고 흡광값을 측정하였다. 정상 대조군으로는 시료와 과산화수소를 처리하지 않은 well을 사용하여 정상 대조군의 세포 생존율을 100%으로 하여 정상 대조군 대비 과산화수소만 처리군 (음성 대조군)과 시료 및 과산화수소 처리군의 ATP 양을 계산하여 결과를 도 4에 나타내었다.In this test example, the main function of mitochondria is ATP synthesis. CCD-986sk cells, which are normal human fibroblasts, were inoculated in a 12-well plate at a concentration of 5X10 4 cells/well, and cultured at 37° C., 5% CO 2 condition for 24 hours. Thereafter, the culture medium was removed, and a sample diluted to a concentration of 100 μg/mL of each of the barley branches and leaf extracts prepared according to Example 1 in a serum-free medium was treated with 1 ml per well 24 Further incubation for hours. Thereafter, cells treated with 100 μM hydrogen peroxide for 1 hour were washed with PBS, and distilled water at 100° C. was added and mixed several times to collect cells. The collected cells were centrifuged at 12,000 x g for 5 minutes at 4°C, and 10 μl of the supernatant obtained and 90 μl of the ATP assay mixture prepared in advance were added, and the absorbance value was measured. As a normal control, using a sample and a well not treated with hydrogen peroxide, the cell viability of the normal control was set to 100%, and the amount of ATP of the hydrogen peroxide-treated group (negative control) and the sample and hydrogen peroxide-treated group compared to the normal control group was calculated and the result was calculated. 4 shows.
실험결과, 인간정상섬유아세포에서 무첨가 대비 과산화수소 (H2O2)에 의해 65.8%로 감소되었던 ATP 합성 정도가 보리밥나무 가지, 잎 추출물을 처리한 결과, ATP 생성 정도가 정상 대조군과 비슷한 수준으로 증가한 것을 확인하였다. As a result of the experiment, the level of ATP synthesis, which had been reduced to 65.8% by hydrogen peroxide (H 2 O 2 ) in normal human fibroblasts compared to no addition, was increased to a level similar to that of the normal control group as a result of treatment with barley branches and leaf extracts. confirmed that.
[시험예 5: 보리밥나무 추출물이 ROS 생성에 미치는 영향 확인][Test Example 5: Confirmation of effect of barley extract on ROS production]
세포 내에 생성되는 활성 산소는 2',7'-dichlorodihydrofluorescein diacetate (H2DCF-DA)를 사용하여 측정하였다. H2DCF-DA는 지용성 물질로, 세포 안으로 쉽게 투과하여 esterase에 의해 가수분해되어 형광물질인 DCF (dichlorofluorescein)를 생성한다. 따라서 이를 측정함으로써 활성산소 생성량을 측정할 수 있다. 물질 처리 및 과산화수소(H2O2) 처리를 통해 얻어진 세포를 DPBS로 두 번 세척 후 25 μ M의 농도로 희석한 H2DCF-DA를 100μL 가하고 37℃, 5 % CO2 조건에서 45 min 간 배양 후, 초기에 활성산소종으로 산화된 DCF의 형광 강도를 형광플레이트리더(Ex = 485 nm, Em = 530 nm)로 측정하였다. 과산화수소만 처리한 well (음성 대조군)의 ROS 생성 정도를 100%로 하여 시료와 과산화수소 모두 처리하지 않은 군과 시료 및 과산화수소 처리 군의 ROS 생성 양을 계산하여 도 5에 나타내었다. Active oxygen generated in cells was measured using 2',7'-dichlorodihydrofluorescein diacetate (H2DCF-DA). H2DCF-DA is a fat-soluble substance that easily penetrates into cells and is hydrolyzed by esterase to generate DCF (dichlorofluorescein), a fluorescent substance. Therefore, it is possible to measure the amount of active oxygen production by measuring it. After washing the cells obtained through material treatment and hydrogen peroxide (H 2 O 2 ) treatment twice with DPBS, 100 μL of H2DCF-DA diluted to a concentration of 25 μM was added and incubated for 45 min at 37°C, 5% CO2 condition, The fluorescence intensity of DCF initially oxidized with reactive oxygen species was measured with a fluorescence plate reader (Ex = 485 nm, Em = 530 nm). Assuming that the ROS generation level of the wells treated with only hydrogen peroxide (negative control) was 100%, the ROS generation amount of the sample and the hydrogen peroxide treatment group was calculated and shown in FIG. 5 .
상기 시험에서 시료는 실시예 1에 따라 제조된 추출물을 각각 100 μg/mL 의 농도 희석한 가지와 잎 추출물, 및 아스코르브산을 의미한다.In the test, the samples refer to eggplant and leaf extracts obtained by diluting the extract prepared according to Example 1 to a concentration of 100 μg/mL, respectively, and ascorbic acid.
실험 결과, 인간정상섬유아세포에서 과산화수소 (H2O2)에 의해 증가되었던 ROS 생성 정도가 아무것도 처리하지 않은 무 첨가 대조 군과 비슷한 수준인 62.1 %, 65.7 %로 감소한 것을 확인하였다.As a result of the experiment, it was confirmed that the level of ROS production increased by hydrogen peroxide (H 2 O 2 ) in human normal fibroblasts was reduced to 62.1% and 65.7%, which were similar to the control group that was not treated with anything.
시험예 1, 2, 3, 4 및 5의 실험 결과에 따르면, 본 발명에 따른 보리밥나무 추출물을 처리 시 과산화수소 (H2O2)로부터 세포를 보호, 미토콘드리아 DNA의 생합성 증가, 미토콘드리아의 주된 기능인 ATP 합성, 그리고 ROS 생성을 감소시키는 효과가 있음을 확인하였다. 따라서, 본 발명에 따른 조성물은 피부 내 산화스트레스로 유도되는 피부 항산화 및 노화를 효과적으로 개선시킬 수 있음을 기대할 수 있다.According to the experimental results of Test Examples 1, 2, 3, 4 and 5, when the barley extract according to the present invention is treated, the cells are protected from hydrogen peroxide (H 2 O 2 ), the biosynthesis of mitochondrial DNA is increased, and ATP, which is the main function of mitochondria It was confirmed that there is an effect of reducing synthesis and ROS production. Therefore, it can be expected that the composition according to the present invention can effectively improve skin antioxidant and aging induced by oxidative stress in the skin.
이하, 상기한 실험예의 결과를 근거로 하여, 보리밥나무 추출물을 이용한 여러 제형 예를 조성하여 제시한다. 그러나 이들 제형예는 본 발명을 설명하기 위한 것으로, 본 발명의 제형이 이들 제형예에만 국한되지 않는 다는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명한 것이다.Hereinafter, based on the results of the above-described experimental examples, various formulation examples using the barley rice tree extract are formulated and presented. However, these formulation examples are for illustrating the present invention, and it is obvious to those skilled in the art that the formulations of the present invention are not limited to these formulation examples.
Claims (15)
상기 피부개선이 항산화 및 항노화인 것을 특징으로 하는 조성물.According to claim 1,
The composition, characterized in that the skin improvement is antioxidant and anti-aging.
상기 조성물은 보리밥나무 산화스트레스에 대한 피부 세포 보호용인 것을 특징으로 하는 조성물.The method of claim 1,
The composition is a composition, characterized in that for protecting skin cells against oxidative stress of barley.
상기 조성물은 미토콘드리아 기능 유지 및 기능 활성화에 의한 피부 개선용인 것을 특징으로 하는 조성물.According to claim 1,
The composition is a composition, characterized in that for improving the skin by maintaining and activating mitochondrial function.
상기 조성물은 피부 주름 개선용인 것을 특징으로 하는 조성물.The method of claim 1,
The composition is a composition, characterized in that for improving skin wrinkles.
상기 조성물은 피부 활력 증진용인 것을 특징으로 하는 조성물.The method of claim 1,
The composition is a composition, characterized in that for enhancing skin vitality.
상기 조성물은 활성산소종(ROS)의 감소에 의한 피부 개선용인 것을 특징으로 하는 조성물.The method of claim 1,
The composition is a composition, characterized in that for skin improvement by the reduction of reactive oxygen species (ROS).
상기 조성물은 보리밥나무 추출물을 상기 조성물 총 중량에 대하여 0.0001~10% 중량%로 포함하는 것을 특징으로 하는 조성물.The method of claim 1,
The composition is characterized in that it comprises 0.0001 to 10% by weight of the barley extract based on the total weight of the composition.
상기 보리밥나무 추출물은 물, 유기용매 또는 이들의 혼합 용매로 추출되는 것을 특징으로 하는 조성물.According to claim 1,
The composition, characterized in that the barley rice extract is extracted with water, an organic solvent, or a mixed solvent thereof.
상기 유기용매는 메탄올, 에탄올, 프로필알코올, 부틸알코올, 글리세린, 프로필렌글리콜 및 부틸렌글리콜 중에서 선택된 1종 이상인 것을 특징으로 하는 조성물.10. The method of claim 9,
The organic solvent is a composition, characterized in that at least one selected from methanol, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol and butylene glycol.
상기 화장료 조성물이 수용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성 함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션 및 스프레이로 구성된 군으로부터 선택되는 어느 하나의 제형인 것을 특징으로 하는 피부개선용 화장료 조성물.12. The method of claim 11,
Any one selected from the group consisting of an aqueous solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray A cosmetic composition for skin improvement, characterized in that it is a formulation.
상기 화장료 조성물은 수중유형 및 유중수형 메이크업베이스, 파운데이션, 스킨커버, 립스틱, 립그로스, 페이스파우더, 투웨이케익, 아이섀도, 치크칼라 및 아이브로우 펜슬류로 이루어진 색조화장료 제형 중에서 선택되는 어느 하나의 제형인 것을 특징으로 하는 피부개선용 화장료 조성물.12. The method of claim 11,
The cosmetic composition is any one formulation selected from the color cosmetics formulations consisting of oil-in-water and water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color and eyebrow pencils. A cosmetic composition for skin improvement, characterized in that
상기 건강기능식품 조성물은 캡슐, 정제, 과립, 분말 또는 음료 형태인 것을 특징으로 하는 피부개선용 건강기능식품 조성물.15. The method of claim 14,
The health functional food composition is a health functional food composition for skin improvement, characterized in that it is in the form of a capsule, tablet, granule, powder or beverage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020210016016A KR20220112447A (en) | 2021-02-04 | 2021-02-04 | Composition for improving skin, comprising Elaeaqnus macrophylla Extract as effective components, Cosmetic composition and Food functional composition including the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020210016016A KR20220112447A (en) | 2021-02-04 | 2021-02-04 | Composition for improving skin, comprising Elaeaqnus macrophylla Extract as effective components, Cosmetic composition and Food functional composition including the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20220112447A true KR20220112447A (en) | 2022-08-11 |
Family
ID=82803226
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020210016016A KR20220112447A (en) | 2021-02-04 | 2021-02-04 | Composition for improving skin, comprising Elaeaqnus macrophylla Extract as effective components, Cosmetic composition and Food functional composition including the same |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR20220112447A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102597791B1 (en) * | 2023-06-02 | 2023-11-06 | 대한민국 | Composition for preventing hair loss or promoting hair growth containing the Elaeagnus macrophylla or the fraction thereof |
| KR102634324B1 (en) * | 2023-04-28 | 2024-02-07 | 김기정 | Composition comprising Dokdo Longleaf Elaeagnus oil for external application for skin |
-
2021
- 2021-02-04 KR KR1020210016016A patent/KR20220112447A/en unknown
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102634324B1 (en) * | 2023-04-28 | 2024-02-07 | 김기정 | Composition comprising Dokdo Longleaf Elaeagnus oil for external application for skin |
| KR102597791B1 (en) * | 2023-06-02 | 2023-11-06 | 대한민국 | Composition for preventing hair loss or promoting hair growth containing the Elaeagnus macrophylla or the fraction thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101971837B1 (en) | Cosmetic composition for improving skin whitening and wrinkle comprising adventitious root extract of Centella asiatica as effective component | |
| KR20100043655A (en) | A cosmetic composition for stabilizing vitamin c | |
| CN102970999A (en) | Skin-whitening composition for external application on skin containing extracts from paper mulberry flowers and fruits | |
| KR20150050310A (en) | Composition for improving skin conditions comprising artemisiae annuae herba, neem leaf, centella asiatica and green tea extract or fraction thereof and method for improving skin conditions using the same | |
| KR102164246B1 (en) | Cosmetic Compositions for Anti-oxidation and Protecting Skin from UV Comprising Complex Extracts of Plants | |
| KR20220112447A (en) | Composition for improving skin, comprising Elaeaqnus macrophylla Extract as effective components, Cosmetic composition and Food functional composition including the same | |
| KR20100042181A (en) | Cosmetic compositions containing extract of rheum undulatum and coptidis rhizoma for skin-protection against uv | |
| KR102525467B1 (en) | Skin external application composition for anti-aging containing Rhodotypos scandens extract | |
| KR102632084B1 (en) | Cosmetic composition for anti-oxidation, skin whitening, and improving of skin wrinkle containing Sanguisorba officinalis extract | |
| KR102255580B1 (en) | Cosmetic composition comprising Ilex paraguayensis extract and Tranexamic acid | |
| KR101299024B1 (en) | Cosmetics composition for skin lightening color, antioxidantsand and method for preparing the same | |
| KR101370990B1 (en) | Anti-heat aging effect of fermented Limonia acidissima extract and its cosmetic usage to improve skin problems | |
| CN106413687B (en) | Product and its preparation method and application containing gadol extract and ginkgo biloba extract | |
| KR20220112446A (en) | Composition for improving skin, comprising Rosa wichuraiana Extract as effective components, Cosmetic composition and Food functional composition including the same | |
| KR101700105B1 (en) | A composition for antioxidating, whitening and improving wrinkle comprising extracts of false indigo | |
| KR100789632B1 (en) | Cosmetic for skin whitening containing a herb extract with inhibitory activity of melanin formation | |
| KR100829718B1 (en) | Cosmetic Composition for Whitening the Skin Comprising the Extract of Ilex rotunda as Active Ingredient | |
| KR102168533B1 (en) | Cosmetic composition for whitening or improving the facial color containing herb extracts | |
| KR20220112449A (en) | Composition for improving skin, comprising Rhododendron weyrichii Maxim. Extract as effective components, Cosmetic composition and Food functional composition including the same | |
| KR20220112448A (en) | Composition for improving skin, comprising Phellinus ribis Extract as effective components, Cosmetic composition and Food functional composition including the same | |
| KR20220112445A (en) | Composition for improving skin, comprising Patrinia Saniculaefolia Hemsl. Extract as effective components, Cosmetic composition and Food functional composition including the same | |
| KR102031289B1 (en) | A cosmetic composition comprising flankton extract for blue light interception | |
| KR20100088463A (en) | Cosmetic composition for whitening the skin comprising the extract of aronia melanocarpa as active ingredient | |
| KR101176525B1 (en) | Cosmetic Composition Comprising Acer tegmentosum Maxim as Active Ingredient | |
| KR101083029B1 (en) | A cosmetic composition containing rice germ and Coptis chinensis extracts for absorbing Ultraviolet |