KR20220085088A - Food additives to improve immune diseases using herbal medicines from Kamigongjindan - Google Patents

Abstract

The present invention relates to a composition for enhancing immunity, and more particularly, it contains extracts of antler, angelica, cornflower oil, goji berry, turmeric, sanyak, dermis, baechohyang, cheonma, cinnamon, omija, red ginseng, maekmundong, and citrus powder in appropriate ingredient ratios. Thus, it exhibits pharmacological activity similar to that of ginseng ginseng such as immunity enhancement, physical strength improvement and fatigue recovery, and can be used for manufacturing gamigong ginseng from which musk has been removed, or as a pharmaceutical preparation or health functional food for immune enhancement and fatigue recovery. is about In particular, the composition for enhancing immunity according to the present invention can be usefully used as a preparation for growing children or adolescents because the taste is sweet and sour and edible is increased when prepared as a pill.

Description

Food additives to improve immune diseases using herbal medicines from Kamigongjindan}

The present invention relates to an immune-enhancing composition containing herbal medicines as main ingredients, and more particularly, extracts of antler, angelica, cornflower, goji berry, turmeric, sanyak, dermis, baechohyang, cheonma, cinnamon, omija, red ginseng, maekmundong and citrus powder. It contains pharmacological activity similar to Gongjindan such as immunity enhancement, physical strength improvement and fatigue recovery by including it in an appropriate ingredient ratio, so it is used for manufacturing Gamigonginseng from which musk has been removed, or as a pharmaceutical preparation or health functional food for immunity enhancement and fatigue recovery. It relates to a possible composition for enhancing immunity.

In recent years of industrialization and changes in the environment, modern people are exposed to various diseases due to reduced immunity due to various causes, and consume a huge amount of money to treat these diseases. In particular, due to recent changes in polluted air and new lifestyles, that is, excessive consumption of instant food and carbonated drinks, etc., abnormalities in the immune system occur, and allergic diseases are continuously increasing. Allergic diseases are caused by excessive stimulation of the human immune system by several allergens, so that TNF-α (tumor necrosis factor-α), IL-1 (interleukin-1), IL-6 (interleukin) -6), prostaglandin (prostagladin), leukotriene (luecotriene), and nitric oxide (NO) by inducing an excessive amount of inflammatory substances such as nitric oxide (NO), which progresses to an inflammatory reaction allergic rhinitis, atopic dermatitis, chronic asthma and immunity It is known to cause hypersensitivity reactions.

In order to prevent and treat these immune diseases, various drugs have been developed, but most drugs are chemical drugs and have side effects, and there is a limitation in not being able to provide a continuous therapeutic effect.

In oriental medicine, the concept of preventive treatment to prevent disease before it occurs was strong, and conventional herbal medicine was such a concept. Gongjindan, a representative oriental medicine prescription drug for these supplements, is a prescription composed of antler, angelicae, cornflower oil, and musk. If you look at the use of Gongjindan to prevent aging and disease, if your energy is weakened due to surgery or a serious illness, or if you are mentally or physically overworked Low energy, severe chronic fatigue, slow recovery even with rest, congenital weakness, weight and numbness due to decreased blood circulation, dizziness and insomnia due to sensitive nerves, excessive stress and drinking It is known to be useful when the liver function is weakened. According to Donguibogam, “In general, when a man has very weak energy in his adulthood, he is not born weak and vain. There are many drugs in the prescriptions to protect eumhyeol, but the efficacy is very weak, so it is difficult to see the effect. However, if the innate vitality is strengthened and the divine beast and shiver go up and down well, the five organs will harmonize themselves and all kinds of diseases will not occur. In such a case, I use this recipe.” Grind antler (baked in boiled milk), 160g each of Angelica keis, and cornsuis oil, and 20g of musk (separately ground), add alcohol, knead it with Sun wheat paste, and then take a pill the size of a Byeok Paulownia seed. 70-100 tablets at a time are to be taken with boiled alcohol or salted water.

However, musk, which is one of the main ingredients of Gongjindan, has had problems in using it because the supply of raw materials is not smooth, the price is also high, and there is a lot of controversy over its authenticity. In addition, although woodhyang, which has been recently used as a substitute for musk, can be used as a medicine, it has the disadvantage that it cannot be developed as a food product.

In addition, most of the pills such as Gongjingin manufactured with these herbal ingredients as the main ingredient have a bitter taste, making it difficult for children and adolescents to eat them.

Accordingly, the present inventors conducted repeated research to develop a new formulation of a herbal composition having a pharmacological effect similar to that of the existing Gongjinjin, while excluding musk. , cinnamon, omija, red ginseng, maekmundong, and tangerine powder extracts in an appropriate ingredient ratio show effects such as immunity enhancement and fatigue recovery. By finding that there is, the present invention was completed.

Accordingly, an object of the present invention is to provide a composition for enhancing immunity comprising extracts of antler, angelica, cornflower oil, goji berry, turmeric, sanyak, dermis, baechohyang, cheonma, cinnamon, omija, red ginseng, maekmundong and citrus powder as active ingredients will be.

Another object of the present invention is to provide a pharmaceutical preparation for preventing or treating immune diseases, comprising the composition and a pharmaceutically acceptable carrier.

Another object of the present invention is to provide a health functional food for improving immune disease and improving fatigue recovery comprising the composition.

In one aspect, the present invention relates to a composition for enhancing immunity comprising an extract of antler, angelica, cornflower oil, goji berry, turmeric, sanyak, dermis, baechohyang, cheonma, cinnamon, omija, red ginseng, maekmundong and citrus powder as active ingredients will be.

Each herbal material included in the composition of the present invention can be used without limitation, such as cultivated or commercially available, and a conventional extraction method in the art can be used for the preparation of the extract. Specifically, by appropriately shredding dried, frozen or natural materials, purified water (water), lower alcohols having 1 to 4 carbon atoms, or their It can be extracted using a mixed solvent. As the extraction method, hot water extraction, cold extraction, reflux cooling extraction, ultrasonic extraction, or the like may be used, preferably hot water extraction.

In addition, in order to purify and concentrate the extract, it may be used after filtration and freeze-drying.

The deer antler contained in the composition of the present invention is a deer hair of Cervus nippon Temminck, Cervus nippon Temminck, C. elaphus Linne, or Daerok C. canadensis Erxleben belonging to the family Cervidae. Dense, not yet ossified, or slightly ossified young horns are cut and dried. Deer antler contains 16 kinds of amino acids, and among them, proline, lysine, and serine are known to have the highest content. Deer antlers contain a large amount of amines, among which spermine, spermidine, and putrescine are abundant. In the middle and lower antlers, ossification has progressed a lot and the content of spermidine is decreased. In addition, it contains collagen, estrone, proteolipid, etc.

Angelica gigas Nakai is a dried root of Angelica gigas Nakai, a perennial herb belonging to the Umbelliferae family. It consists of essential oil and decucin, which are aromatic ingredients, beta-sitosterol, sucrose, nicotinic acid, and folic acid, which have a calming effect.

Cornus officinalis Sieb, a deciduous small tree belonging to the Cornaceae family, Cornus officinalis Sieb. et. It is the dried fruit of Zucc., and it contains cornin, that is, verbenalin, saponin, tannin, ursolic acid and vitamin A, and the oil of the seed contains palmitin acid, olein acid, linol acid, etc.

Lycium chinense Mill belongs to the Solanaceae family. Or other mature fruits of plants and animals, containing Carotene 3.39mg%, vitamin B1 0.23mg%, vitamin B2 0.33mg%, nicotin acid 1.7mg%, vitamin C 3mg%, β-sitosterol, linoleic acid, etc. has been

Turmeric belongs to the ginger family (生薑科; Zingiberaceae). or 溫鬱金 C. wenyujin Y.H. Chen et C. Ling, 薑黃 C. longa L. 廣西莪朮 C. kwangsiensis S. Lee et C. F. Liang, 我朮 C.aeruginosa Roxb. It is a dried saffron from C. chuanyujin C. K. Hsieh et H. Zhang, and the main component contains 6.1% of mulberry, among which 0.8% of d-camphene, 2.5% of d-camphor, and sesquiterpene (l-α-curcumene, l-β-curcumene) 65.5%, sesqui-terpene alcohol 22%, etc., and curcumin 0.3%, demethoxycurcumine, bisdemethoxycurcumin, turmerone, arturmerone, etc. Its action is similar to that of turmeric. In addition, 澱粉 30-40%, 脂肪油 3%, 黃色色素 and carvone and phellandrene are contained.

Saponin, mucus, cholin, starch (16%), glycoprotein from the rhizomes of the hemp Dioscorea batatas Decne. and free amino acids, abscisin Ⅱ, polyphenol oxidase, vitamin C (5mg%), 3,4-dihydroxy-phenylethylamine (dopamine), and mannan and phytinic acid in the mucus.

The dermis is the rind of the mature fruit of Citrus unshiu Markovich (= Citrus reticulata Blanco) belonging to the Rutaceae family and a related plant. , myoinositol, and vitamin C, and also contains a lot of flavonoide glycosides.

Kwakhyang is the above-ground part of the outpost of Agastache rugosa (Fisch. et Meyer) O. Kuntze belonging to the Lamiaceae family, Labiatae. It is collected between June and July and dried in the sun or shaded. Kwakhyang contains 0.28% of essential oil, and methylchavicol as the main component accounts for more than 80%, anethole, anisaldehydel, α-limonene, ρ-metlioxycinnaldehyde, α-pinene, β-pinene, 3-ocatanone, 3-ocatanol, ρ- It contains cymene, 1-octen-3-ol, linalool, 1-caryophyllene, β-elemen, β-humulene, β-farnesene, γ-cadinene, and calamenene.

Chunma is the dried tuber root of Gastrodia elata Bl. belonging to the Orchidaceae family. contains etc. The gastrodin content, known as the main component, varies depending on the production area, but it is about 0.25 to 0.67%.

Cinnamon is the bark of the broiler Cinnamomum cassia Presl belonging to the Lauraceae family, and contains 1~2% of essential oil, and its main component is 75~90% of cinnamaldehyde. It is hot in nature and has a bitter and sweet taste.

Schisandra chinensis Baill. Schisandra chinensis Baill. or S. sphenanthera Rehd. et Wils. et Wils. contains about 3% of essential oil, and various sesquicarene, β2-bisabclene, β-chamigrene, α-ylangene, etc. are contained in the essential oil.

Dried fruit contains 12% citral, 10% malic acid, and a small amount of tartaric acid, as well as fructose and resin.

Ginseng is a processed ginseng root of Panax ginseng C.A Mey belonging to the Araliaceae family. When the stem and leaves are dry in autumn, it is called white ginseng, and it is called white ginseng, and the fine root is removed and steamed and dried. Dried ginseng after processing with sugar is called sugar ginseng. Ginseng contains about 5.22% of ginsenoside. Ginseng ginsenoside is a mixture of more than 13 kinds of saponin, and among them, the content of ginsenoside Rb1, Rc and Rg1 is relatively high.

There are three types of genin containing saponin: protopanaxadiol, protopanaxatriol, and oleanol acid. In addition, it contains about 0.05% of essential oil, which contains β-elemen, which is the source of a unique fragrance, and panaxynol, which easily turns into resin. In addition, panax acid is a mixture of palmitin acid, stearin acid, olein acid, linol acid, etc., and is produced by hydrolysis of lipids contained in roots. In addition, β-sitosterol, stigmasterol, campesterol, and daucosterol were also isolated, and cholin was contained in 0.1~0.2%, which is a potentiating component of ginseng. It also contains many kinds of amino acids and peptides, vitamins A·Bl·B2·C, and glucose, fructose, maltose, and sucrose.

Liriope platyphylla Wang et Tang or Ophiopogon japonicus Ker-Gawl belonging to the family Liliaceae. It contains sugars such as glucose and fructose, sucrose β-sitosterol, β-sitosterol-β-D-glucose and other mucilage as main components. Recently, steroids, saponins, pohiopogonin A, B, C, D, and oligosaccharides were discovered.

The content of each component contained in the composition for enhancing immunity of the present invention is antler 5-25 mass ratio, angelica 5-25 mass ratio, cornflower oil 5-25 mass ratio, goji berry 5-25 mass ratio, turmeric 5-15 mass ratio, sanyak 5-15 Mass ratio, dermis 5-15 mass ratio, baechohyang 5-15 mass ratio, cheonma 1-5 mass ratio, cinnamon 1-5 mass ratio, omija 1-5 mass ratio, red ginseng 1-5 mass ratio, maekmundong 1-5 mass ratio, and citrus powder 1-5 mass ratio It may include, and preferably, antler 10-15 mass ratio, Angelica 10-15 mass ratio, cornflower oil 10-15 mass ratio, Goji berry 10-15 mass ratio, turmeric 8-12 mass ratio, acid about 8-12 mass ratio, dermis 8-12 mass ratio , Baechohyang 8-12 mass ratio, Chunma 1-4 mass ratio, cinnamon 1-4 mass ratio, Schisandra 1-4 mass ratio, red ginseng 1-4 mass ratio, Maekmundong 1-4 mass ratio and citrus powder 1-4 mass ratio, more preferably is antler 12 mass ratio, angelica 12 mass ratio, cornflower oil 12 mass ratio, goji berry 12 mass ratio, turmeric 10 mass ratio, ginseng 10 mass ratio, dermis 10 mass ratio, baechohyang 10 mass ratio, cheonma 2 mass ratio, cinnamon 2 mass ratio, omija 2 mass ratio, red ginseng 2 mass ratio, maekmundong It contains 2 mass ratio and 2 mass ratio of citrus powder.

When the composition for enhancing immunity of the present invention has the above-described component ratio, it exhibits optimal immunity enhancing efficacy without exhibiting cytotoxicity. In addition, when the composition of the present invention is provided in the form of a pill, when it has the above-mentioned composition ratio, it has excellent texture and high edibility.

In addition, the composition for enhancing immunity of the present invention is characterized in that it has an effect of enhancing immunity, enhancing physical strength, and relieving stress.

The composition for enhancing immunity of the present invention contains deer antler, cornflower oil and angelicae, which are known as major components of Gongjinjin, a representative oriental medicine prescription drug, and other medicinal substances, goji berries, turmeric, ginseng, ginseng, baechohyang, cheonma, cinnamon, omija, red ginseng, By including maekmundong and citrus powder in an appropriate ratio, it exhibits an immune-enhancing action, so it can be used for manufacturing Gamigongdiagnosis excluding musk. Accordingly, it is preferred that the composition of the present invention be processed in particular in the form of pills. In the form of pills, after extracting and concentrating the herbal medicine, each medicinal material is finely milled using a grinder, the extract and powder are mixed, and the powder mixed with the medicinal solution is put into a grinder again to be finely milled and filtered through a sieve of 300 to 500 mesh to fine particles. It can be made with honey and made into Hwan(丸), Dan(丹), Won(元), etc. At this time, for the convenience of taking, it is manufactured in units of 3.75 ~ 6g, and one to two tablets can be consumed per day. In addition, medicines made from pills, sweets, and circles can be packaged again using edible gold leaf (or silver foil). Gold or silver has been used to make pills since ancient times, and has been used as an auxiliary material in prescriptions such as Gongjinjin or Woohwangcheongsimwon because of its effect of stabilizing the mind and preventing insects. In addition, in another form, the process of making the pill is the same, and it can be used by extracting a drug that has been used as a dye from the past and has an insect repellent and antiseptic effect and coating it on the surface. A natural coating may be applied. Pills prepared with the composition for enhancing immunity of the present invention have a sweet and sour taste and thus increase edibility, so they can be used as preparations for children and adolescents as well as adults.

The composition of the present invention may be used as a pharmaceutical preparation or health functional food for enhancing immunity.

Accordingly, in another aspect, the present invention relates to a pharmaceutical preparation for preventing or treating immune diseases, comprising the composition for enhancing immunity and a pharmaceutically acceptable carrier.

In the present invention, "immune disease" includes various inflammatory diseases, allergic diseases and cancer caused by abnormalities in the immune system of a living body, for example, cold, asthma, pneumonia, allergic rhinitis, allergic conjunctivitis, atopic dermatitis, allergy, rheumatoid arthritis, Alzheimer's disease, autoimmune thyroid disease, inflammatory bowel disease, aplastic anemia, lupus erythematosus and psoriasis, etc., but are not limited thereto.

The composition of the present invention may be provided as a pharmaceutical composition for the prevention or treatment of immune diseases, and may be formulated together with a carrier. The pharmaceutical composition of the present invention may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories, and sterile injection solutions according to conventional methods, respectively. In addition, carriers, excipients and diluents that may be included in the composition comprising the extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate , calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, it is prepared using commonly used diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations contain at least one excipient, for example, starch, calcium carbonate, sucrose, lactose, gelatin, etc. in the extract. prepared by mixing In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Liquid formulations for oral use include suspensions, solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients, for example, wetting agents, sweeteners, fragrances, preservatives, etc. may be included. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, Witepsol, macrogol, cacao butter, laurin, glycerogelatin, and the like can be used.

When the composition of the present invention is used as a pharmaceutical composition, the extract may be formulated to be included in an amount of 0.0001 to 50% by weight, preferably 0.1 to 20% by weight, based on dry weight, based on the total weight of the total composition.

The preferred dosage of the composition of the present invention varies depending on the patient's health status and weight, the degree of disease, drug form, administration route and period, but may be appropriately selected by those skilled in the art. However, for a desirable effect, the composition of the present invention may be administered in an amount of 0.0001 to 1000 mg/kg, preferably 0.1 to 100 mg/kg, based on the dry weight of the total extract, once a day or divided into several times a day.

The route of administration of the composition of the present invention may be administered through various routes, either oral or parenteral, as long as it can reach the target tissue, and specifically, oral, rectal, topical, intravenous, intraperitoneal, intramuscular, intraarterial, It may be administered in a conventional manner via transdermal, intranasal, inhalation, intraocular or intradermal routes. Preferably, it is administered orally.

The composition of the present invention is applicable to any animal, and the animal includes not only humans and primates, but also domestic animals such as cattle, pigs, sheep, horses, dogs and cats.

In addition, the composition of the present invention can be used alone for prevention and treatment of immune diseases such as inflammatory diseases and allergic diseases, or in combination with methods using surgery, hormone therapy, drug therapy, and biological response modifiers.

In another aspect, the present invention relates to a health functional food for improving immune disease and improving fatigue recovery comprising the composition for enhancing immunity.

The composition of the present invention may be added to a health functional food for the purpose of preventing or improving immune diseases, such as inflammatory diseases and allergic diseases, or promoting recovery from fatigue. At this time, each material included in the composition may be used in a manner in which the material itself is powdered and additionally added in addition to the extract form.

The health functional food may be manufactured by adding other food materials according to the marketability and consumer preference of the final product.

The effective addition amount of the composition may be appropriately determined depending on the purpose of use (prophylaxis, health or therapeutic treatment), preferably 0.01 to 90% by weight, more preferably 5 to 90% by weight based on the total raw material dry weight of the crude drug 30% by weight may be added.

The composition for enhancing immunity of the present invention can be prepared as a health functional food in the form of a formulation by further including a food additive, which is pharmaceutically acceptable, and can be a tablet, capsule, pill, liquid, etc. do.

In addition, there is no particular limitation on the type of food to which the composition can be added. For example, there are beverages, tea, bread, chocolate, candies, snacks, and vitamin complexes, and includes all functional foods in a conventional sense.

In addition, the health functional food of the present invention includes various flavoring agents, natural carbohydrates, sweeteners, vitamins, electrolytes, colorants, pectic acid, alginic acid, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents and the like may be further contained. In particular, as the natural carbohydrate, monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol can be used. , As the sweetener, natural sweeteners such as taumatine and stevia extract, or synthetic sweeteners such as saccharin and aspartame can be used. The proportion of these additives is not very important, but may be selected in the range of about 0.01 to 10 g per 100 ml of the composition of the present invention.

According to the present invention, the composition for enhancing immunity comprising extracts of antler, angelica, cornflower oil, goji berry, turmeric, ginseng, ginseng, baechohyang, cheonma, cinnamon, omija, red ginseng, maekmundong and citrus powder as active ingredients while exhibiting immune enhancing activity It also has excellent human stability, so it can be usefully used in the manufacture of pharmaceutical preparations or health functional foods for the prevention or treatment of immune diseases.

In addition, the composition for strengthening immunity of the present invention not only exhibits effects such as immunity enhancement, nourishment tonic, physical strength improvement and fatigue recovery improvement for test takers, but also has excellent texture when processed in the form of pills. can

In addition, the pill prepared from the composition of the present invention has a sweet and sour taste, so the edibility is increased, so it can be used as a preparation for not only adults, but especially children and adolescents.

<Examples 1 to 16>

Deer antler, angelica, sansuyu, goji berry, turmeric, sanyak, ginseng, baechohyang, cheonma, cinnamon, omija, red ginseng, maekmundong, clove, peony, sukhukhwang, bokryeong, health, woosul, jujube and aeyeop were dried in the shade and chopped. Citrus powder was used. These were mixed according to Table 1 below in the same mass ratio, respectively. Distilled water 10 times the total weight of each mixture was added to the mixture, boiled for 10 hours, and hot water extraction was performed. The hot water extract was filtered and then concentrated using a vacuum concentrator.

<Experimental Example 1>

Blood from healthy adults is collected and allowed to clot at room temperature for about 15 minutes, then the clotted blood is cut and left at room temperature for about 5 minutes. This blood was left at 4°C for about 20 minutes and then centrifuged (3,000 rpm, 20 minutes, 4°C) to separate the serum, then dispensed 1 mL each into microcentrifuge tubes and stored frozen at -70°C, used in the test.

GVB++ (gelatin veronal buffer, pH 7.4, 0.1% gelatin, 0.15 mM Ca++, 0.5 mM Mg++ containing) and 50 μL of each of the extracts of Examples 1 to 16 were mixed in the serum, followed by a primary reaction at 37° C. for 30 minutes. Then, add 350 μL of GVB++, serially dilute it 10 to 160 times, add 750 μL of GVB++ and 250 μL of sheep sensitized red blood cells (Ig M-sensitized sheeperythrocyte, EA cell, 1×10 ⁸ cells/mL). Secondary reaction was carried out at 37°C for 50 minutes. Then, 2.5 mL of PBS (phosphate buffered saline, pH 7.4) was added to stop the reaction. The reaction solution was centrifuged at 2,000 rpm for 10 minutes, and the residual hemolytic activity was measured by measuring the absorbance of the obtained supernatant at 412 nm.

Anti-complement activity was expressed as inhibition of 50% total complement hemolysis (ITCH ₅₀ , %) against total complement hemolysis (50% total complement hemolysis, TCH ₅₀ , %) of a negative control group in which only normal human serum, GVB++, and distilled water were reacted. it was Polysaccharide K (PSK) was used as a positive control.

<Equation 1>

ITCH ₅₀ (%)=(TCH _{50 of control - TCH 50 of} Example / TCH ₅₀ of Example) X 100

As a result, it was confirmed that the ITCH ₅₀ of Examples 2, 3, 8, 12, 13 and 16 was high (Table 2). Therefore, subsequent experiments were carried out using antler, angelica, cornflower, goji berry, turmeric, sanyak, ginpi, baechohyang, cheonma, cinnamon, omija, red ginseng, maekmundong, and tangerine with high anticomplement activity.

<Examples 17-32>

Deer antler, angelica, sansuyu, goji berry, turmeric, sanyak, ginseng, baechohyang, cheonma, cinnamon, omija, red ginseng, maekmundong and tangerine were mixed in the mass ratio shown in Table 3 below. Distilled water 10 times the total weight of each mixture was added to the mixture, boiled for 10 hours, and hot water extraction was performed. The hot water extract was filtered and then concentrated using a vacuum concentrator.

<Experimental Example 2>

The spleen is a major lymphoid organ that collects antigens from blood, matures B and T lymphocytes, and differentiates lymphocytes after being stimulated by antigens. The proliferation of lymphocytes in the spleen has a very important meaning for the immune system.

6-8 week old female Balb/c mice were acclimatized to 23 °C, 40-60% humidity, and light-dark cycle conditions for 1 week. Then, the extracts of Examples 17-32 were added at a dose of 70 ml/kg, administered together with a general feed (purina) for 1 month, and blood was collected. After staining with fluorescent dyes (FITC, PE), the number of total T lymphocytes and total B lymphocytes in the blood was measured using flow cytometry (Table 4).

*B lymphocytes and T lymphocytes are expressed as the number of cells X 10 ⁵ .

As a result, it was confirmed that the immune enhancing effect was increased when the extracts of Examples 17, 21, 22, 25, 26, 30, 31 and 32 were administered. In summary, antler, angelica, cornflower oil, goji berry, turmeric, sanyak, ginseng, baechohyang, cheonma, cinnamon, omija, red ginseng, maekmundong and citrus powder 5-25: 5-25: 5-25: 5-25 : 10~15: 10~15: 10~15: 10~15: 1~5: 1~5: 1~5: 1~5: 1~5: It is effective to extract hot water by mixing in a mass ratio of 1~5 was good

Claims (6)

Contains extracts of deer antler, angelica, sansuyu, goji berry, turmeric, sanyak, ginseng, baechohyang, cheonma, cinnamon, omija, red ginseng, maekmundong and citrus powder as active ingredients,
The content of each component is: antler 10-15 mass ratio, angelica 10-15 mass ratio, cornflower oil 10-15 mass ratio, goji berry 10-15 mass ratio, turmeric 8-12 mass ratio, sanyak 8-12 mass ratio, dermis 8-12 mass ratio, baechohyang 8 ~12 mass ratio, cheonma 1-4 mass ratio, cinnamon 1-4 mass ratio, omija 1-4 mass ratio, red ginseng 1-4 mass ratio, maekmundong 1-4 mass ratio, and citrus powder 1-4 mass ratio, characterized in that it is included, immunity enhancement for composition. The composition for enhancing immunity according to claim 1, wherein the composition is for the production of Gamigondiagnosis excluding musk. The composition for enhancing immunity according to claim 1, wherein the extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The composition for enhancing immunity according to claim 1, wherein the composition is in the form of a pill. A cold, asthma, pneumonia, allergic rhinitis, allergic conjunctivitis, atopic dermatitis, allergy, rheumatoid arthritis, Alzheimer's disease, autoimmune thyroid disease, inflammatory bowel disease, A pharmaceutical agent for preventing or treating immune diseases selected from the group consisting of aplastic anemia, lupus erythematosus and psoriasis. A health functional food for improving immune disease and promoting recovery from fatigue, comprising the composition of claim 1.