KR20220082673A - Arthrobacter pascens S29-14 strain for controlling fire blight and uses thereof - Google Patents

Abstract

The present invention relates to an Arthrobacter pascens strain for controlling fruit burns and uses thereof, and the Arthrobacter pascens S29-14 strain having excellent antibacterial activity against fruit burns (deposited) No.: KACC 92320P), a composition for controlling fruit burns containing the strain or a culture solution of the strain, and the like, and to a method for controlling fruit burns using the strain.

Description

Arthrobacter pascens S29-14 strain for controlling fire blight and uses thereof for controlling fruit burns and uses thereof

The present invention relates to a strain of Arthrobacter pascens for controlling fire blight and uses thereof.

Fire blight is a disease in which the leaves, flowers, branches, stems, and fruits of more than 180 species of plants belonging to the Rosaceae change as if they were burned by fire, and the plants wither and die. It occurs in common apple, pear, and quince trees. Bacterial plant disease, orchard burns, was first discovered in the United States in 1780, but since no treatment or vaccine has been developed yet, incineration or burial is required to prevent its spread. The problem does not end with burial. After burial, it is not possible to grow plants susceptible to fruit burns on the same land for three years. The cultivation restriction period is three years, but it may take longer for the orchard to actually return to its former appearance.

Worldwide, fire blight has occurred in 30 countries in most parts of the world, including the Americas, Europe, Asia, and Africa. In Korea, the outbreak was reported in Anseong-si, Gyeonggi-do in 2015, Cheonan-si, Chungcheongnam-do, and Jecheon-si, Chungcheongbuk-do. In the same year, cases of burn disease were shared in Cheonan, Chungnam, Iksan, Jeollabuk-do, Anseong, Paju, Icheon, Yeoncheon, Yangju, and Gwangju in addition to the Chungbuk region in the same year. As it is gradually spreading across the country, it is becoming a threat to not only fruit orchard farmers in North Chungcheong this year, but also apple and pear farms across the country.

The technical problem to be achieved by the present invention is to provide a strain effective for controlling fruit burns, a composition for controlling fruit burns using such strains, and a method for controlling fruit burns using the same.

The present invention provides an Arthrobacter pascens S29-14 strain (Accession No.: KACC 92320P) for controlling fruit burns.

The strain is characterized in that it has 16S rRNA of the nucleotide sequence shown in SEQ ID NO: 1.

It provides a composition for controlling fruit burn disease comprising all of the strain, the culture solution of the strain, or the strain, and the culture solution of the strain as an active ingredient.

The target plants of the fruit burn disease control are apple trees, pear trees, quince trees, apricot trees, peach trees, plum trees, plum trees, aronia, cherry trees, raspberries, meadowsweet trees, roses, rowan trees, grasshoppers, myongjae trees, It provides a composition for controlling fruit burns comprising at least one of brier, strawberry, and cherry tree.

The target plant for controlling the fruit burns provides a composition for controlling the fruit burns of an apple tree.

Any one or more selected from the group consisting of Arthrobacter pascens S29-14 strain (Accession No.: KACC 92320P), a culture solution of the strain, a composition comprising the strain, and a composition comprising the culture solution of the strain It provides a method for controlling fruit burn disease comprising the step of treating the plant, the area around the plant, or both.

The plant provides a method for controlling fruit burn disease, which is an apple tree.

At least one of the strain, the culture solution of the strain, the composition comprising the strain, and the composition comprising the culture solution of the strain is sprayed or inoculated to the plant.

The plant provides a method for controlling fruit burn disease comprising at least one of a leaf, a fruit, and a seedling as an area to be inoculated.

The present invention provides a composition for controlling fruit burns, including the Arthrobacter pascens S29-14 strain (Accession No.: KACC 92320P), the strain or a culture solution of the strain, for controlling fruit burns, using the same To provide a method for controlling fruit burns. Fruit burn disease can be controlled by using the strain according to the present invention.

1a is a photograph showing apple leaves treated with TSB (Tryptic Soy Broth), which is a culture medium, as a negative control.
1b is a photograph showing an apple leaf inoculated with a pathogen suspension on petioles after treatment with TSB, which is a culture medium, as a control.
1c is a photograph showing an apple leaf inoculated on petioles with the pathogen suspension after treatment with the antibacterial active strain suspension as an experimental group (S29-14).
2A is a photograph showing apple fruits treated with TSB, a culture medium, as a negative control.
2B is a photograph showing a cross-section of an apple fruit treated with TSB, which is a culture medium.
3A is a photograph showing apple fruits inoculated with a pathogen suspension after treatment with TSB, which is a culture medium, as a control.
3B is a photograph showing a cross-section of an apple fruit inoculated with a pathogen suspension after treatment with TSB, a culture medium.
4A is a photograph showing apple fruits inoculated with the pathogen suspension after treatment with the antibacterial active strain suspension as an experimental group (S29-14).
Figure 4b is a photograph showing a cross-section of an apple fruit inoculated with the pathogen suspension after treatment with the antibacterial active strain suspension.
5a is a photograph showing apple seedlings treated with TSB, a culture medium, as a negative control.
Figure 5b is a photograph showing the front side of the leaf of the apple seedlings treated with the culture medium TSB.
Figure 5c is a photograph showing the reverse side of the leaf of the apple seedlings treated with TSB, a culture medium.
Figure 5d is a photograph showing the cutting of the stem of the apple seedlings treated with the culture medium TSB.
6a is a photograph showing an apple seedling inoculated with a pathogen suspension after treatment with TSB, a culture medium, as a control.
Figure 6b is a photograph showing the front side of the leaf of the apple seedlings inoculated with the pathogen suspension after treatment with the culture medium TSB.
Figure 6c is a photograph showing the reverse side of the leaf of the apple seedlings inoculated with the pathogen suspension after treatment with the culture medium TSB.
Figure 6d is a photograph showing the cutting of the stem of the apple seedlings inoculated with the pathogen suspension after treatment with TSB, a culture medium.
7a is a photograph showing an apple seedling inoculated with a pathogen suspension after treatment with an antibacterial active strain suspension as an experimental group.
Figure 7b is a photograph showing the front side of the leaf of the apple seedlings inoculated with the pathogen suspension after treatment with the antimicrobial active strain suspension.
Figure 7c is a photograph showing the reverse side of the leaves of the apple seedlings inoculated with the pathogen suspension after treatment with the antimicrobial active strain suspension.
Figure 7d is a photograph showing the cutting of the stem of the apple seedlings inoculated with the pathogen suspension after treatment with the antibacterial active strain suspension.
8 is a close-up view of the 16S rRNA nucleotide sequence of S29-14.

The present invention is Asrobacter pascens ( Arthrobacter pascens ) S29-14 strain (Accession No.: KACC 92320P), a composition for controlling fire blight comprising the strain or a culture solution of the strain, the strain or the above It is characterized in that it is a method for controlling an overwater burn disease using a composition or the like.

Hereinafter, the present invention will be described in detail with reference to the drawings.

The present invention provides an Arthrobacter pascens S29-14 strain (Accession No.: KACC 92320P), characterized in that it has 16S rRNA of the nucleotide sequence shown in SEQ ID NO: 1.

The present invention is Arthrobacter pascens S29-14 strain (Accession No.: KACC 92320P), the culture solution of the strain, or the strain, and for controlling fruit burns containing all of the culture solution of the strain as an active ingredient A composition is provided.

As used herein, “plant” refers to any living organism belonging to the plant kingdom (i.e., any genus/species within the plant kingdom), such as, but not limited to, trees, herbs, shrubs, grasses, vines, ferns, moss and green algae. does not Therefore, representative plants to which the composition can be applied include brassica, bulbous vegetables, grains, citrus, cotton, gourds, fruit vegetables, leaf vegetables, legumes, oil seed plants, peanuts, pome fruits, root vegetables, tuber vegetables, caliber vegetables , nuclear fruits, tobacco, strawberries, berries, and various ornamental plants. For example, according to an embodiment of the present invention, it may be applied to apples.

As used herein, the term “culture medium” refers to a result obtained by inoculating a strain on a medium and culturing for a certain period of time, the culture medium itself culturing the strain according to the present invention in a suitable liquid medium, the filtrate obtained by filtering or centrifuging the culture medium to remove the strain (filtration solution or centrifuged supernatant), a cell lysate obtained by sonicating the culture solution or treating the culture solution with a lysozyme, and a concentrate obtained by concentrating the culture solution, filtrate, and lysate, etc. It is not limited. In addition, the composition for probiotic or antibacterial activity may contain a culture such as a solid medium culture or a dried product or extract of the culture medium, but is not limited thereto, and may further include a suitable excipient or carrier.

The composition of the present invention may be prepared in the form of a liquid fertilizer, and may be used in the form of a powder by adding an extender thereto, or may be formulated and granulated. However, it is not particularly limited to the formulation.

The term “fruit burn disease” is an infectious disease affecting some groups of plants belonging to the Rosaceae family. The causative pathogen is Erwinia amylovora , and leaves, flowers, branches, stems, and fruits are burned. It is a disease that causes the tissues to turn black and gradually dry up, as if they were wearing a sweatshirt.

The term “control against waterweed” is used herein in its ordinary meaning to refer to the antibacterial activity of a strain against waterweed. Therefore, "Utilizing the antibacterial activity of the strain corresponding to fruit burn" is usually to control the onset by administering the strain of the present invention or a substance produced by the strain when the plant has an orchard strain. means to be

The composition for controlling fruit burns of the present invention can control fruit burns.

The composition for controlling fruit burns of the present invention is an apple tree, a pear tree, a quince tree, an apricot tree, a peach tree, a plum tree, a plum tree, an aronia tree, a cherry tree, a raspberry, a seaweed tree, a rose, a rowan tree, a grass tree, a myongja tree. Erwinia amylovora ( Erwinia amylovora ) It is possible to control any one or more fruit burn diseases selected from the group corresponding to the host plant, such as brier, strawberry, and cherry tree. For example, according to one embodiment of the present invention, the composition for controlling fruit burns can control fruit burns of apple trees.

The composition for controlling fruit burns of the present invention may further include other optional components used for controlling plant burns in the art to the above-described strain of the present invention, the culture medium of the strain, or a mixture thereof.

The composition for controlling fruit burns of the present invention may include other beneficial microorganisms, microbial active substances, organic fertilizers, and chemical fertilizers.

The present invention is Asrobacter pascens ( Arthrobacter pascens ) S29-14 strain (Accession No.: KACC 32320P), a culture solution of the strain, a composition comprising the strain, and a composition comprising the culture solution of the strain selected from the group consisting of It provides a method for controlling fruit burns, comprising the step of treating any one or more of plants, areas around plants, or both to control fruit burns.

The part of the plant to which the present invention is applied is not limited. Accordingly, it includes the whole plant as well as parts of the plant such as seeds, shoots, stems, roots, leaves, fruits (fruits), and their whole as well as parts thereof. For example, according to an embodiment of the present invention, it can be applied to leaves, fruits, and seedlings.

The present invention can be carried out by immersing or spraying, that is, spraying, in seeds or plants, the strain, the culture medium in which the strain is cultured, and the composition using the strain. In the case of the immersion method, the culture solution and formulation can be sprayed on the soil around the plant or the seeds are soaked in the culture solution and composition and shaken inoculated.

The surrounding area to which the present invention is applied means a range in which the composition of the present invention can exert an effect, and includes both above ground and underground. For example, the area around a plant, plant part, or fruit may be within about 2 m, within about 1 m, within about 70 cm, within about 50 cm, within about 25 cm, within about 10 cm, and within about 5 cm.

The method of the present invention comprises continuously applying the composition at any time during the life cycle of the plant, during one or more stages of the life cycle of the plant, or at regular intervals in the life cycle of the plant, or continuously throughout the life of the plant. include Accordingly, the composition can be applied as needed. The composition may be applied to the plant, for example, during growth, before and/or during flowering, and/or before and/or during seed development. In one example, the composition may be applied before, during and/or immediately after the plant is transplanted from one location to another, such as in a greenhouse or hotbed, into the field. As another example, each composition may be applied to the plant multiple times at a desired interval period.

The fruit burn disease control method of the present invention is an apple tree, a pear tree, a quince tree, an apricot tree, a peach tree, a plum tree, a plum tree, an aronia tree, a cherry tree, a raspberry, a seaweed tree, a rose, a rowan tree, a grass, and a myongja tree. Erwinia amylovora among rosaceae, such as brier, strawberry, and cherry tree, can control fruit burn disease of any one or more plants selected from the group corresponding to the host plant. For example, according to one embodiment of the present invention, it is possible to control the fruit burn disease of apples.

The present invention is Asrobacter pascens ( Arthrobacter pascens ) S29-14 strain (Accession No.: KACC 92320P), a culture solution of the strain, a composition comprising the strain, and a composition comprising the culture solution of the strain selected from the group consisting of It provides a method for controlling fruit burn disease, comprising the step of treating any one or more of the plant, the area around the plant, or both.

Asrobacter pascens S29-14 strain (Accession No.: KACC 92320P) according to an embodiment of the present invention described above, a culture solution of the strain, a composition comprising the strain, and a culture solution of the strain In the case of using a composition to be used, apples and pears, etc. Erwinia amylovora ( Erwinia amylovora ) It is possible to control fruit burns caused by growing on host plants.

Hereinafter, the present invention will be described in more detail through examples. However, these are only for describing the present invention in more detail, and the scope of the present invention is not limited thereto.

[Example 1] Isolation of microorganisms, selection of antibacterial active strain S29-14

In order to select fruit-burning control microorganisms, 1,758 microorganisms were isolated from samples such as soil, shoots, flowers, stems, and fruits using the dilution smear method. Among isolated microorganisms, 10 μl of the isolated strain was inoculated into a solid medium (2.3% NA (Nutrient Agar) + 5% sucrose) to select microorganisms exhibiting antibacterial activity against the fruit tree burn pathogen ( Erwinia amylovora ), and then inoculated at 28°C for 24 hours. cultured. S29-, in which a clear zone is formed by pouring 0.6% soft agar containing pathogens (OD ₆₀₀ = 0.1) into a plate in which the isolate is cultured, and culturing at 28 ° C. for 2 days 14 strains were selected.

[Example 2] Test for apple shoots

A strain showing antibacterial activity against orchard burn pathogens was inoculated into TSB (Tryptic Soy Broth) and cultured at 28° C. for 24 hours, and apple shoots were tested as test subjects. The new shoots of the three-year-old grafted apple tree were collected and prepared by removing foreign substances from the surface with a clean brush. After making a wound on the petiole of the apple shoots with a needle, the wounded apple shoots were immersed in an antibacterial active strain suspension (OD ₆₀₀ = 0.1) for 30 seconds, then placed on filter paper and dried. In the case of a negative control group and a control group, TSB, a culture medium of the selection strain, was treated. The dried apple leaves were placed in a wet chamber-treated square plate and placed in a constant temperature room for 24 hours (26°C, 16 hours Light/8 hours Dark (=16L/8D)). 10 μl of the pathogen suspension (10 ⁶ cfu/mL) was injected and inoculated into the petiole of injured apple leaves of the control group and the experimental group (S29-14) using a needle-free syringe, and the severity of the disease was observed in a constant temperature room for 7 days. .

Figure 1a is a photograph showing the negative control apple leaves treated with TSB (Tryptic Soy Broth), which is a culture medium.

Figure 1b is a photograph showing a control apple leaf inoculated with the pathogen suspension on the petiole after treatment with the culture medium TSB.

Referring to FIG. 1B, in FIG. 1B as a control group, it was found that light brown symptoms were widely spread along the main vein of the leaf from the petiole inoculated with the pathogen.

Referring to FIGS. 1A and 1B , it was possible to estimate that the pathogen migrated, passed the petiole, and expanded to a wide area along the main vein of the leaf during the onset of fruit burn disease.

Figure 1c is a photograph showing the experimental group apple leaves inoculated with the pathogen suspension on the petiole after treatment with the antibacterial active strain suspension.

Referring to Fig. 1c, in Fig. 1c of the experimental group (S29-14), almost no symptoms were observed in the petiole of the shoot inoculated with the pathogen, and accordingly, it was confirmed that the disease did not progress to the main vein of the leaf.

Referring to FIGS. 1B and 1C , it was confirmed that the leaves of the experimental group (S29-14) had significantly fewer light brown symptoms compared to the control group. Fruit burn disease was controlled as much as the lesion extension area was insufficient compared to the control group, and accordingly, the S29-14 strain was judged to have an excellent control effect on fruit burn disease targeting apple leaves.

[Example 3] Test for apple fruits

A strain exhibiting antibacterial activity against orchard burn pathogens was inoculated into TSB (Tryptic Soy Broth) and cultured at 28° C. for 24 hours, and apple fruits were tested as test subjects. Immature apples with surface disinfection were cut, and the cut site was immersed in the antibacterial active strain suspension (OD ₆₀₀ = 0.1) for 5 seconds, then placed on filter paper and dried. In the case of the negative control group and the control group, TSB, which is the culture medium of the selection strain, was treated. The dried apple fruits were placed in a dish treated with wet room and placed in a constant temperature room for 24 hours (26°C, 16L/8D). After inoculating 2 sites with 10 μl of the pathogen suspension (10 ⁶ cfu/mL) into the flesh of apple fruits of the control group and the experimental group (S29-14), the disease was observed in a constant temperature room for 7 days.

2A is a photograph showing apple fruits treated with TSB, a culture medium.

2B is a photograph showing a cross-section of an apple fruit treated with TSB, which is a culture medium.

Referring to FIGS. 2A and 2B , the degree of browning of the negative control apple fruits, which were oxidized by air and were overall light brown, were confirmed. More specifically, it was observed that the browning area was uniformly browned to light brown and the browning area was limited to the surface.

3A is a photograph showing apple fruits inoculated with a pathogen suspension after treatment with TSB, a culture medium.

3B is a photograph showing a cross-section of an apple fruit inoculated with a pathogen suspension after treatment with TSB, a culture medium.

Referring to FIGS. 3A and 3B , the degree of browning of the control apple fruit, which was entirely dark brown or partially turned black due to orchard burns, was confirmed. More specifically, ooze occurred on the surface of the fruit, and in the same way that pathogens are inoculated through a fire in nature and the pathogens spread to the fruit tree along the ovary, it is severely browned from the apple stem along the ovary to the core of the fruit. A change to black was observed.

Referring to FIGS. 2A to 3B , the degree of browning occurring in apple fruits at the onset of water burn disease was observed compared to the degree of browning caused by air. Accordingly, by observing the degree of browning, the extent of the progression of the water burn disease was confirmed.

Figure 4a is a photograph showing the apple fruit inoculated with the pathogen suspension after treatment with the antimicrobial active strain suspension.

Figure 4b is a photograph showing a cross-section of an apple fruit inoculated with the pathogen suspension after treatment with the antibacterial active strain suspension.

Referring to FIGS. 4A and 4B , the degree of browning of some speckled brown apple fruits was confirmed as the fruit burn disease was somewhat controlled by the S29-14 strain. More specifically, brown spots were formed as traces of slight water needles at the site of pathogen inoculation.

3A to 4B, compared to the control group, the degree of browning was much less in the experimental group (S29-14), and there was no trace of the browning part digging into the fruit in the areas other than the inoculation site, so it was confirmed that some fruit burns were controlled.

[Example 4] Assay for seedlings

A strain exhibiting antibacterial activity against orchard burn pathogens was inoculated into TSB (Tryptic Soy Broth) and cultured at 28° C. for 24 hours, and apple rootstock (M9) seedlings were tested as test subjects. The upper part of the apple seedling was cut with pruning shears to give a wound. In the case of the experimental group (S29-14), the antimicrobial active strain suspension (OD ₆₀₀ = 0.1) was sprayed on the wounded seedlings by 10 mL each. In the case of the negative control group and the control group, TSB, which is the culture medium of the selection strain, was treated. The seedlings of the negative control group, the control group, and the experimental group (S29-14) were placed in an acrylic cage and kept at 28° C. and 100% humidity for 24 hours. After 24 hours, the seedlings of the control group and the experimental group were inoculated by spraying a suspension (10 ⁶ cfu/mL) of the fruit burnt fungus. The 3rd week of treatment was investigated whether the disease occurred.

Figure 5a is a photograph showing the apple seedlings treated with TSB, a culture medium.

Figure 5b is a photograph showing the front side of the leaf of the apple seedlings treated with the culture medium TSB.

Figure 5c is a photograph showing the reverse side of the leaf of the apple seedlings treated with TSB, a culture medium.

Figure 5d is a photograph showing the cutting of the stem of the apple seedlings treated with the culture medium TSB.

Referring to FIGS. 5A to 5D , it was possible to confirm the shape of the seedlings, which are negative controls, the front side of the leaf, the back side of the leaf, and the normal shape of the stem.

Figure 6a is a photograph showing an apple seedling inoculated with a pathogen suspension after treatment with TSB, a culture medium.

Figure 6b is a photograph showing the front side of the leaf of the apple seedlings inoculated with the pathogen suspension after treatment with the culture medium TSB.

Figure 6c is a photograph showing the reverse side of the leaf of the apple seedlings inoculated with the pathogen suspension after treatment with the culture medium TSB.

Figure 6d is a photograph showing the cutting of the stem of the apple seedlings inoculated with the pathogen suspension after treatment with TSB, a culture medium.

6A to 6D, the pathogen inoculated on the control seedlings moves through the xylem and the xylem of the stem also shows a brown necrosis. When reaching the leaf along the stem, the front of the leaf along the main vein of the leaf, It was confirmed that the entire apple seedling was infected with fruit burn disease, as brown spots on the back showed symptoms of expanding.

7A is a photograph showing an apple seedling inoculated with a pathogen suspension after treatment with an antimicrobial active strain suspension.

Figure 7b is a photograph showing the front side of the leaf of the apple seedlings inoculated with the pathogen suspension after treatment with the antimicrobial active strain suspension.

7c is a photograph showing the reverse side of the leaf of the apple seedling inoculated with the pathogen suspension after treatment with the antimicrobial active strain suspension.

Figure 7d is a photograph showing the cutting of the stem of the apple seedlings inoculated with the pathogen suspension after treatment with the antibacterial active strain suspension.

7A to 7D , it was confirmed that the experimental group, the seedlings, the front side of the leaf, the back side of the leaf, and the shape of the stem were close to those of the negative control group. The browning area, leaf ulcer symptoms, and stem necrosis, which are the characteristics of fruit burn disease, were not observed, and it was confirmed that the fruit burn disease was controlled.

The results of comparing the leaf rates (%) of the negative control group, the control group, and the experimental group are shown in Table 1 below.

As shown in Table 1, it was confirmed that the experimental group treated with the S29-14 strain had a leaf rate of 0% in the same way as the negative control group.

[Example 5] Identification of S29-14 strain having an effect of controlling fruit burns

The 16S rRNA portion of the S29-14 strain was sequenced, and 16S rRNA information of the S29-14 strain was identified through phylogenetic analysis.

8 is a close-up view of the 16S rRNA nucleotide sequence of S29-14.

Referring to Figure 8, the S29-14 strain Neighbor-joining (Neighbor-joining) in the phylogenetic tree obtained through the Asrobacter pascens ( Arthrobacter pascens ) species and the closest taxonomic position. Therefore, the strain S29-14 strain of the present invention was confirmed to be the most closely related to Asrobacter pascens ( Arthrobacter pascens ).

In addition, the S29-14 strain was deposited and given an accession number KACC 92320P.

Asrobacter pascens according to an embodiment of the present invention as described above ( Arthrobacter pascens ) When using the S29-14 strain (Accession No.: KACC 92320P), the culture of the strain, the composition including the strain, and the composition including the culture solution of the strain, or applying the above-mentioned fruit burn disease control method, to the plant Erwinia amylovora , which infects and causes fruit burns, can be controlled in an environmentally friendly way.

In the above, the applicant has described preferred embodiments of the present invention, but these embodiments are only one embodiment that implements the technical idea of the present invention, and any changes or modifications as long as the technical idea of the present invention is implemented. should be construed as within the scope.

Agricultural Biotechnology Research Institute KACC92320P 20201019

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Claims (9)

Arthrobacter pascens for controlling fruit burns S29-14 strain (Accession No.: KACC 92320P). According to claim 1,
The strain is Asrobacter pascens ( Arthrobacter pascens ) S29-14 strain (Accession No.: KACC 92320P), characterized in that it has 16S rRNA of the nucleotide sequence shown in SEQ ID NO: 1. The strain according to claim 1, the culture solution of the strain, or the strain, and a composition for controlling fruit burn disease comprising all of the culture solution of the strain as an active ingredient. 4. The method of claim 3,
The target plants of the fruit burn disease control are apple trees, pear trees, quince trees, apricot trees, peach trees, plum trees, plum trees, aronia, cherry trees, raspberries, meadowsweet trees, roses, rowan trees, grasshoppers, myongjae trees, A composition for controlling fruit burns comprising at least one of brier, strawberry, and cherry tree. 5. The method of claim 4,
The target plant for controlling the fruit burns is the apple tree, the composition for controlling fruit burns. Any one or more selected from the group consisting of Arthrobacter pascens S29-14 strain (Accession No.: KACC 92320P), a culture solution of the strain, a composition comprising the strain, and a composition comprising the culture solution of the strain A method for controlling fruit burns comprising the step of treating a plant, an area around the plant, or both. 7. The method of claim 6,
The plant is an apple tree fruit burn disease control method. 7. The method of claim 6,
At least one of the strain, the culture solution of the strain, the composition comprising the strain, and the composition comprising the culture solution of the strain is sprayed or inoculated to the plant. 9. The method of claim 8,
The plant is an area to be inoculated, the fruit burn disease control method comprising at least one of leaves, fruits, and seedlings.

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