KR20220072065A - Composition for improving sleep containing coniferous extract - Google Patents

Abstract

The present invention relates to a sleep improvement composition containing a conifer extract, and more particularly, to a sleep-improving natural material containing a conifer extract containing a large amount of alpha-pinene that helps to stabilize the mind and body. It is a composition for improving sleep that exhibits a sleep improvement effect so as to induce will be.

Description

Composition for improving sleep containing coniferous extract

The present invention relates to a sleep improvement composition containing a conifer extract, and more particularly, to a sleep-improving natural material containing a conifer extract containing a large amount of alpha-pinene, which is helpful for mental and physical stability, and induces a good night's sleep for a comfortable sleep for a long time. It is a composition for improving sleep that exhibits a sleep improvement effect so as to induce .

The present invention is a research carried out with the support of Gyeonggi Provincial Economic Science Promotion Agency with the resources of Gyeonggi Province in 2020 [Research project name: Announcement of additional implementation plan for the 2020 Gyeonggi Province sleep products and services commercialization support project (R&D field), research project name: Development of a source introduction for sleep-inducing forest healing products from ingredients derived from pine trees in Gapyeong, Gyeonggi-do.

It is well known that sleep has a very important effect on health. Sleep is an active state in which important physiological functions are operated by the brain for the brain. It is an adaptive behavior maintained as a high level of physiological function rather than a simple downtime, and is a biological defense technology. In particular, for humans with a developed cerebrum, the quality of sleep has a large influence on the quality of life, so getting a good night's sleep is very important for improving the quality of life.

As it is known that sleep disorders have a very negative effect on health, various types of drugs for the treatment of sleep disorders have been proposed.

As a conventional technique for improving sleep disorders, folding screen extract, octopus calligraphy extract, citrus peel extract, etc. have been developed as compositions for improving or treating sleep disorders. (Korean Patent Registration No. 10-2157248, Korean Patent Publication No. 10- See 2019-0106782, Korean Patent Registration No. 10-2139253, etc.)

In addition, a composition for treating sleep disorder using a chemical (Korean Patent Publication No. 10-2019-0024593) or a composition for treating sleep disorder using polygamma glutamic acid obtained from a fermented product of Bacillus subtilis has also been proposed. ( Korean Patent Registration No. 10-2149404)

However, these existing compositions have a problem in that they are difficult to apply for the treatment of sleep disorders because the manufacturing cost is high or the effect is insignificant.

Therefore, in a situation where the number of people complaining of sleep disorders is increasing day by day due to the increasing number of causes of various stresses, it is urgent to develop a composition that can be widely used in various classes through practical use because it is easy to supply raw materials and has an excellent sleep disorder effect.

Korean Patent Registration No. 10-2157248 Korean Patent Publication No. 10-2019-0106782 Korean Patent Registration No. 10-2139253 Korean Patent Publication No. 10-2019-0024593 Korean Patent Registration No. 10-2149404

In order to improve the problems of the prior art as described above, in the present invention, unlike the existing natural products, it is a problem to discover a natural material that is abundant in nature and is easy to supply and manufacture raw materials and has excellent sleep improvement effect.

Accordingly, an object of the present invention is to provide a composition for improving sleep or improving sleep disorders obtained from a conifer extract widely present in nature.

In addition, another object of the present invention is to provide a composition for improving the quality of sleep or sleep disorders containing pine pine extract as an active ingredient.

In addition, another object of the present invention is a composition for various uses, such as a health food composition for improving sleep or sleep disorder improvement, a composition for external application for skin, or a pharmaceutical composition for preventing or treating diseases caused by sleep disorders, containing a conifer extract as an active ingredient is to provide

In order to solve the above problems, the present invention provides a composition for preventing and improving sleep improvement or sleep disorder-related diseases or symptoms containing a conifer extract containing alpha pinene as an active ingredient.

In addition, the present invention provides a composition for external application for skin for improving sleep or improving sleep disorders, comprising a conifer extract containing alpha pinene as an active ingredient.

In addition, the present invention provides a health functional food composition for preventing or improving sleep improvement or sleep disorder-related diseases or symptoms, comprising a conifer extract containing alpha pinene as an active ingredient.

In addition, the present invention provides a dry functional food composition for enhancing concentration and improving sleep or preventing and improving diseases or symptoms related to sleep disorders, comprising a pine tree extract as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for the prevention and treatment of diseases or symptoms related to a decrease in concentration or sleep disorder comprising a pine tree extract as an active ingredient.

The present invention is a natural material having a sleep improvement effect containing alpha pinene of a conifer extract, and the sleep improvement composition according to the present invention has a sleep improvement effect as well as a sleep disorder-related disease, a concentration improvement effect through improvement of concentration, and a decrease in concentration It is also effective in the treatment of various diseases that may be caused by it.

In addition, the composition made of the natural material of the present invention has an effect of inducing comfortable sleep, such as good sleep and sleep quality improvement, insomnia, and can be applied as a composition for external application to the skin, so it can be applied as beauty and cosmetics, It can be used in various fields such as food, health functional food, and medicine.

In addition, the present invention can be obtained as an extract from a coniferous tree abundantly present in nature, particularly preferably from a cypress tree, so that it is easy to supply and supply raw materials, and there is a commercially very useful advantage in that it can be manufactured economically.

1 is a graph showing the superiority of the effect of shortening the elevation delay time for the pine tree extract of the present invention compared to the control group.
Figure 2 is a graph showing that the sleep efficiency of the pine nut extract of the present invention is superior to that of the control group by comparison.
3 is a graph showing the superiority of the sleep tension for the pine pine extract of the present invention compared to the control group.
4 is a graph showing that the degree of toss and downs during sleep for the Pine pine extract of the present invention is superior to that of the control group.
5 is a graph showing that the overall feeling of sleep for the pine nut extract of the present invention is superior to that of the control group.

Hereinafter, the present invention will be described in more detail as one embodiment as follows.

The present invention relates to a natural material for improving sleep containing a conifer extract containing alpha pinene as an active ingredient.

The natural material of the present invention can be applied as a composition for external application to the skin and can be provided as a very useful bio-cleaning agent as a composition for food or a pharmaceutical composition.

According to a preferred embodiment of the present invention, the conifers used in the extract of conifers may be byproducts of conifers.

According to a preferred embodiment of the present invention, as the conifers for obtaining the conifer extract, for example, pine, metasequoia, blue globular, fir, pine, cypress, Japanese cedar, birch, birch, and arborvitae. , at least one of cypress trees may be used. More preferably, when a cypress tree is used as a conifer, a more excellent effect can be obtained.

Today, modern people suffer from chronic fatigue and stress due to heavy work, and as one of the ways to relieve fatigue and stress, the number of people who enjoy forest bathing is increasing. It is known that one of the reasons why forest bathing is good for fatigue and stress is because of phytoncides that are generated in food to resist pathogens, pests, and fungi, and these phytoncides are composed of terpenes, phenolic compounds, alkaloids, and glycosides. it was known

However, among the beneficial ingredients represented by these natural phytoncides, terpenes may have beneficial effects on human health, but terpenes generated from conifers in particular show very excellent effects.

Therefore, according to a preferred embodiment of the present invention, especially the conifer extract, more preferably the pine nut extract, exhibits a more excellent effect. In particular, according to the present invention, the sleep improvement effect, the sleep disorder improvement effect, the diseases resulting therefrom, and the concentration It was confirmed that the conifer extract, especially the pine pine extract, showed a very good effect on diseases caused by improvement or decreased concentration.

According to a preferred embodiment of the present invention, as a by-product of conifers used in the coniferous extract, one or more of stems, leaves, roots, immature conifers and mature conifers may be used as an extract. More preferably, an extract of the by-product of pine nuts may be used, and preferably, the conifers remaining after removing the pine nuts from the immature conifers or mature conifers or pine nuts may be used.

According to one embodiment of the present invention, it has been found that cypress has no toxicity to the human body, but produces less phytoncide than general conifers. In particular, the phytoncide of Pine pine has the highest content of alpha-pinene, which stimulates the cerebral cortex to increase concentration and exercise, and also contains 3-carene, bonyl-acetate, etc., which are known to give the unique efficacy of forest fragrance.

In particular, according to a preferred embodiment of the present invention, among the conifer extracts, the pine pine extract calms the central nervous system and gives a pleasant feeling, and helps to extend the sleep time and take a comfortable sleep. In addition, pine pine extract has a strong antibacterial action that does not cause resistance in the human body, and the natural substance pine pine extract has almost 90% house dust mite repellent effect, so there are no side effects and it is very effective in preventing allergies.

In the present invention, while conducting continuous research to verify whether various substances have a deep sleep effect, it was found that alpha-pinene in pine nut extract had a sedative effect when administered to experimental mice. However, specifically, it was confirmed that although alpha-pinene has a sedative effect at a low concentration, for example, about 25-80 mg/kg, the effect of improving sleep is significantly superior at a high concentration of about 100 mg/kg or more. did

In particular, according to a preferred embodiment of the present invention, it was confirmed that the sleep improvement effect was very excellent in the conifer extract containing alpha pinene, and more preferably, the pine pine extract was significantly superior.

According to a preferred embodiment of the present invention, the coniferous extract of the present invention may be used as a component obtained by extraction using any one of immature conifers, mature conifers, leaves, branches, roots, and bark. More specifically, it contains various physiologically active substances such as alpha-pinene, beta-pinene, di-limonene, 3-carene, etc. as a component obtained using conifers, and reduces stress from free radicals, eliminates insomnia, or improves sleep quality. There is a function.

According to a preferred embodiment of the present invention, the sleep improvement effect of the conifer extract has the effect of increasing the amount of sleep without impairing sleep intensity by, for example, prolonging the sleep duration, and improving the quality of sleep, etc. and can prevent or treat sleep disorders.

The conifer extract of the present invention has been confirmed to be superior to zolpidem, which is widely known for sleep improvement or treatment of sleep disorders, and unlike conventional drugs, it is a natural material, so it is related to sleep improvement, sleep disorders or unstable life, reduced concentration, etc. without side effects. It is confirmed that it can be very preferably applied to the prevention and treatment of diseases.

According to a preferred embodiment of the present invention, it is possible to provide a sleep improvement natural material containing a conifer extract containing alpha pinene as a composition capable of inducing quality sleep and improving sleep disorders or anxiety.

In addition, according to a preferred embodiment of the present invention, it is possible to provide a food composition or health functional food composition for preventing and improving sleep disorders, including a sleep improvement natural material containing a conifer extract containing alpha pinene.

In addition, according to a preferred embodiment of the present invention, it is possible to provide a pharmaceutical composition for the prevention and treatment of sleep disorders comprising a sleep improvement natural material containing a conifer extract containing alpha pinene.

On the other hand, according to a preferred embodiment of the present invention, it is possible to provide a composition for external application for skin including a sleep improving natural material containing a conifer extract containing alpha pinene.

According to a preferred embodiment of the present invention, the composition for external application for skin is preferably a composition for external application for skin containing in an amount of 80 to 100% by weight based on the total weight of the composition containing the natural material.

According to a preferred embodiment of the present invention, the food composition of the present invention includes processed forms of all natural materials such as food, functional food, nutritional supplement, health food, and food additive. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.

For example, as a health food, pine nut extract can be prepared in the form of tea, juice, and drink for drinking, or it can be consumed by granulation, encapsulation, and powder. In addition, it can be prepared in the form of a composition by mixing the pine nut extract of the present invention with a known active ingredient known to be effective in enhancing concentration, preventing or improving symptoms of sleep disorders.

In addition, as food, non-alcoholic beverages, alcoholic beverages, fruits and their processed foods (eg, canned fruit, bottled, jam, marmalade, etc.), fish, meat and their processed foods (eg, ham, sausage corn beef, etc.) ), breads and noodles (eg udon noodles, soba noodles, ramen, spaghetti, macaroni, etc.), fruit juice, various drinks, cookies, syrup, dairy products (eg butter, cheese, etc.), edible vegetable oils and fats, margarine, vegetable protein, retort Food, frozen food, various seasonings (eg, soybean paste, soy sauce, sauce, etc.) can be prepared by adding pine nut extract. In addition, in order to use the pine pine extract of the present invention in the form of a food additive, it may be prepared and used in the form of a powder or a concentrate.

In the food composition of the present invention, the preferred content of the pine nut extract may be 0.01 to 100% by weight based on the total weight of the food composition.

The pharmaceutical composition according to the present invention may include a pharmaceutically effective amount of pine nut extract alone or may further include one or more pharmaceutically acceptable carriers, excipients or diluents. As used herein, “pharmaceutically acceptable” means a non-toxic composition that is physiologically acceptable and does not inhibit the action of the active ingredient when administered to humans and does not normally cause allergic reactions such as gastrointestinal disorders, dizziness, or similar reactions. say

Examples of the carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, malitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose , polyvinylpyrrolidone, water, methyl hydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil. In addition, the pharmaceutical composition may further include a filler, an anti-aggregating agent, a lubricant, a wetting agent, a fragrance, an emulsifying agent, and a preservative.

As used herein, the term “pharmaceutically effective amount” refers to an amount that exhibits a higher response than that of the negative control group, and preferably refers to an amount sufficient to exhibit the effect of improving concentration and preventing and treating symptoms caused by sleep disorders.

The pharmaceutically effective amount of the pine needle extract according to the present invention is 0.01 to 200 mg/day/kg body weight. However, the pharmaceutically effective amount may be appropriately changed according to various factors such as the type of disease and its severity, the patient's age, weight, health status, sex, administration route, and treatment period.

In addition, the pharmaceutical composition of the present invention can be formulated using methods known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal. The formulation can be powder, granule, It may be in the form of tablets, emulsions, syrups, aerosols, soft or hard gelatin capsules, sterile injectable solutions, or sterile powders.

The route of administration of the composition of the present invention is not limited thereto, but may be administered orally or parenterally. Parenteral routes of administration may include multiple routes, such as, for example, transdermal, nasal, intraperitoneal, intramuscular, subcutaneous, or intravenous.

The pharmaceutical composition of the present invention may be administered in combination with a known compound having the effect of enhancing concentration and/or preventing or treating sleep disorder-related diseases or symptoms.

As described above, the conifer extract of the present invention, more preferably the pine pine extract, can be usefully applied to health foods and medicines, external preparations for skin, etc. for the prevention and treatment of diseases or symptoms related to sleep disorders, regardless of age, for both men and women.

According to a preferred embodiment of the present invention, the sleep improvement composition containing the conifer extract according to the present invention may be formulated in a composition for external application to the skin such as perfume and cosmetics, and may also be formulated as a food composition. The blending amount may be appropriately selected and blended to achieve the desired effect according to conventional techniques in the art, and preferably may be mixed in an amount of 0.01 to 40% by weight based on the total weight of the composition.

According to a preferred embodiment of the present invention, the external preparation for skin is not limited in its formulation and can be formulated in any formulation well known in the art. Examples of dosage forms include packs, ointments, lotions, solubilizers, suspensions, emulsions, creams, gels, sprays, pastes, warning agents, patches or water pastes and the like.

In addition, liquid, solid, gel, etc. may be filled in a container such as a membrane and used.

According to a preferred embodiment of the present invention, in the present invention, the food composition measures EEG, safety, and electrocardiogram during sleep in order to investigate the sleep improvement effect of pine nut extract, polysomnography test and sleep evaluation. did

According to a preferred embodiment of the present invention, as can be seen from the results of the following test examples, by using the pine nut extract sleep improvement composition of the present invention, it can be confirmed from the polysomnography results that the sleep efficiency is increased and the elevation delay time is shortened. . In addition, from the subjective evaluation results conducted after sleep, it can be confirmed that the decrease in tension, the decrease in the degree of tossing and turning, and the overall feeling of satisfaction with sleep are high.

below. The present invention will be described in more detail by way of examples, but the present invention is not limited to these examples.

Test Example 1: Sleep improvement effect experiment using pine pine extract and pine tree extract

In order to confirm the sleep improvement effect of the pine tree extract, the sleep induction time and sleep time of the animal model mice induced with pentobarbital were measured to confirm the significance of the sleep improvement effect of the pine tree extract through a comparative experiment with the pine extract, a species of coniferous tree. measured. Specifically, 45 mg/kg of pentobarbital was administered intraperitoneally, and pine pine extract and pine pine extract were orally administered and tested.

Sleep was defined as the time when the clove reflex disappeared, and the waking time was set as the time the animal returned to the clove.

In order to confirm the sleep improvement effect using pine pine extract and pine tree extract, the results of pentobarbital sleep test after oral administration of 100 mg/kg of each extract to mice are shown in Table 1 below.

control group Sleep induction time (sec) Sleep time (seconds) pine extract 154.22±24.3090 2461.37±246.9644 Pine Tree Extract 129.55±18.2904 5406.00±455.8588

As shown in Table 1 above, the sleep induction time of mice orally administered pine pine extract after intraperitoneal administration of pentobarbital to mice was 129.55 ± 18. sec), a slight decrease was observed.

In addition, the sleep time of the mice administered the pine pine extract to the animal model was 5406.00 ± 455.8588 seconds), which was significantly increased compared to the sleep time of the mice orally administered with the pine extract 2461.37 ± 246.9644 seconds). .

These results show that the pine nut extract has the effect of shortening the sleep induction time and, in particular, significantly increasing the sleep time.

Through these results, it was confirmed that the pine nut extract can shorten the sleep induction time and extend the sleep time for sleep disorders, thereby confirming that the pine nut extract has a sleep improvement effect.

Test Example 2: Measurement of sleep effect of pine pine extract sleep improvement composition material

In order to objectively evaluate the sound sleep effect of the pine pine extract (conifer extract with pine nuts removed) sleep improvement composition material according to the present invention, using the pine pine extract sleep improvement composition material prepared in Table 1 above, EEG, safety level during sleep , an electrocardiogram was measured to conduct polysomnography and subjective evaluation of sleep. The specific method is as follows.

1. Subject

The subjects of the study were 200 women in their 10s to 40s who were healthy, currently not taking drugs, and were not intoxicated.

Subjects were instructed to refrain from excessive exercise or excessive drinking on the day before the experiment, and to refrain from smoking, beverages, drugs, and chewing gum that could affect the central nervous system and sense of smell on the day of the experiment. As an experiment targeting adult women, it was reported that there is a correlation between changes in the sense of smell according to a woman's sexual cycle, and a woman's menstrual cycle and mood.

2. Experimental sample

As a comparative example, a non-scented condition and pine pine extract sleep improvement composition material were used as comparative examples. A constant scent was presented to the sleep laboratory, and polysomnography was performed by measuring brain waves (EEG), safety level (EOG), and electrocardiogram (ECG) during sleep.

3. Experiment site

The experiment was carried out in the sleep laboratory (4.8 m x 3 m x 2.4 m) built by the Institute of Standards and Science, and a soundproof device is installed inside to exclude the influence of the external environment during the experiment. In the sleep laboratory, constant temperature and constant humidity were maintained, and the temperature was 24 ℃ and the humidity was 40%.

4. Measurement method

For physiological signals during sleep, electroencephalogram (EEG), safety level (EOG), and electrocardiogram (ECG) were measured using a polysomnography device. Subjective evaluation of sleep was written after the experiment was completed with respect to the sleep felt by the subjects.

5. Experiment sequence

The polysomnography test was conducted three times under the conditions of the control example and the example.

In order to exclude the effect on sleep, samples were randomly conducted for each subject, and before this experiment, they were asked to sleep in the sleep laboratory 1 to 2 times in order to adapt to the sleep laboratory. The sleep time was 2 hours during the day, and each subject was allowed to sleep at the same time. In addition, subjective evaluation of sleep after sleep was collected through questionnaire.

6. Analysis method

While the subject was sleeping, physiological signals were measured using a polysomnography device, and sleep stages were analyzed using the measured signals. The sleep stage was determined using the international standard method of Rechtschaffen and Kales (1968).

Using the divided sleep stages, sleep latency and sleep efficiency were compared. In addition, after sleeping under the given conditions, subjective feelings were collected through a questionnaire and statistical analysis was performed using the t-test using the SPSS program.

7. Experimental results

go. sleep latency

Condition control group 1 control group 2 control group 3 Experimental group 1 Experimental group 2 Experimental group 3 Elevation delay time average value (minutes) 35.12 34.89 35.77 29.21 29.45 29.33

As can be seen in Table 2 and Figure 1, the average of the elevation delay time was shorter in the experimental group containing the pine tree extract than in the control group not containing the pine pine extract, which is why the experimental group containing the pine nut extract was put to sleep within a short time. means to lift

me. sleep efficiency

Sleep efficiency is the ratio of total sleep time to time in bed, and the ratio of sleep time during the experimental period was compared.

The results for the sleep efficiency are shown in Table 3 and FIG. 2 below.

Condition control group 1 control group 2 control group 3 Experimental group 1 Experimental group 2 Experimental group 3 Sleep efficiency (%) 55.40 56.22 55.20 63.70 63.0 63.2

As can be seen in Table 3 and Figure 2, when looking at the average of sleep efficiency, the experimental group containing the pine tree extract had higher sleep efficiency than the control group, which was longer than the experimental group containing the pine tree extract had no fragrance. means to go to sleep while

All. subjective evaluation

① level of tension

Table 4 and Figure 3 below are charts showing the degree of tension during sleep compared to normal sleep.

Condition control group 1 control group 2 control group 3 Experimental group 1 Experimental group 2 Experimental group 3 Tension level compared to usual (points) 0.92 0.65 0.52 1.12 1.04 1.17

As can be seen in Table 4 and Figure 3, the average sleep efficiency was higher in the experimental group including the pine nut extract sleep improvement composition material than in the control group, which was found in the experimental example containing the pine pine extract sleep improvement composition material. It means going to sleep for a longer period of time than when you are not.

② Degree of tossing

Table 5 and Figure 4 below are charts showing the degree of tossing and turning during sleep compared to usual.

Condition control group 1 control group 2 control group 3 Experimental group 1 Experimental group 2 Experimental group 3 Degree of tossing and turning compared to usual (points) -0.14 -0.20 -0.18 0.85 0.88 0.84

As can be seen in Table 5 and FIG. 4, in the control condition, they answered that they tossed and turned more than usual, and in the experimental group condition, they answered that they tossed and turned less than usual.

③ Overall sleep level

Tables 6 and 5 below are charts showing the overall feeling of sleep compared to usual.

Condition control group 1 control group 2 control group 3 Experimental group 1 Experimental group 2 Experimental group 3 Overall sleep satisfaction (points) 0.32 0.52 0.48 0.96 0.94 0.97

As can be seen in Tables 6 and 5 above, in the control condition, a similar level of sleep was taken compared to usual, but in the experimental group condition, it was answered that they had a good sleep as usual. Overall sleep satisfaction was found to be very good.

According to the following composition, various formulations of health functional food, pharmaceutical composition, and external preparation for skin were prepared.

The pharmaceutical composition or health functional food or formulation example of the composition for external application for skin of the present invention is described below, but the present invention is not intended to be limited thereto, but only to be described in detail.

[Formulation Example 1] Preparation of tablets

120 mg of coniferous extract

100 mg corn starch

Lactose 100 mg

2 mg magnesium stearate

After mixing the above ingredients, tablets are prepared by tableting according to a conventional manufacturing method of tablets.

[Formulation Example 2] Preparation of capsules

120 mg of coniferous extract

3 mg of crystalline cellulose

100 mg corn starch

2 mg magnesium stearate

According to a conventional capsule preparation method, the above ingredients are mixed and filled in a gelatin capsule to prepare a capsule.

[Formulation Example 3] Preparation of injection

120 mg of coniferous extract

mannitol 180 mg

3000 mg of sterile distilled water for injection

26 mg

26 mg

According to a conventional method for preparing injections, the content of the above ingredients per 1 ampoule is prepared.

[Formulation Example 4] External preparation for skin

100% by weight of coniferous extract

The prepared coniferous extract can be used as it is as an external preparation for skin.

Claims (9)

A composition for preventing or improving sleep improvement or sleep disorder-related diseases or symptoms, comprising a conifer extract containing alpha pinene as an active ingredient. A composition for external application to the skin for improving sleep or improving sleep disorders, comprising a conifer extract containing alpha-pinene as an active ingredient. The composition for external application for skin according to claim 2, wherein the coniferous tree is cypress. The composition for external application for skin according to claim 2, wherein the composition for external application for skin contains the conifer extract in an amount of 80 to 100% by weight of the total weight. A cosmetic composition comprising the composition for external application for skin according to any one of claims 2 to 4. A pharmaceutical composition for epidermal use for treating a sleep disorder disease comprising the composition for external application for skin according to any one of claims 2 to 4. A health functional food composition for preventing or improving sleep improvement or sleep disorder-related diseases or symptoms, comprising a conifer extract containing alpha pinene as an active ingredient. A health functional food composition for preventing or improving concentration enhancement, sleep improvement, or sleep disorder-related diseases or symptoms comprising pine pine extract as an active ingredient. A pharmaceutical composition for the prevention or treatment of diseases or symptoms related to reduced concentration or sleep disorders, comprising a pine tree extract as an active ingredient.

Images