KR20210114325A - Prebiotics comprising Pear Extract, Synbiotics Compositions comprising the Same and Uses thereof - Google Patents

Abstract

The present invention relates to a prebiotic composition containing a pear extract as an active ingredient. It is ascertained that a pear extract promotes the growth of Lactobacillus acidophilus and Streptococcus thermophilus strains in a medium for intestinal beneficial bacteria and is substantially equivalent to fructooligosaccharide and galactooligosaccharide, which are representative prebiotics, in terms of functions thereof. Accordingly, a prebiotic composition containing a pear extract as an active ingredient, a synbiotics composition comprising the prebiotic composition, and a functional food composition comprising the synbiotics composition are provided.

Description

Prebiotics comprising pear extract, synbiotic composition comprising same, and uses thereof

The present invention relates to a prebiotic composition comprising a pear extract as an active ingredient.

It is known that various microorganisms inhabit the intestines of mammals including humans, and there are about 300 types of bacteria and about 100 trillion bacteria. In the intestine, beneficial bacteria and harmful bacteria for the human body are balanced in a certain ratio. being the cause

Probiotics are produced from lactic acid bacteria preparations or components of lactic acid bacteria, which are beneficial bacteria, and examples of lactic acid bacteria include Bifidobacterium genus and Lactobacillus genus. The probiotic function inhibits the growth of pathogens in the intestine, produces substances that regulate the immune ability of intestinal epithelial cells or immune cells, inhibits the growth of pathogens by competing with pathogens, and secondaryly prevents antibiotic treatment or changes in the external environment. It is known to maintain the homeostasis of the intestinal flora.

The effect of probiotics has been proven through various experiments based on the idea that if the number of harmful microorganisms decreases by increasing the number of useful microorganisms in the intestine of the genus Bifidobacterium or Lactobacillus, it will be useful in maintaining intestinal homeostasis. However, not all probiotics are always useful, and it is known that even intestinal bacteria of the same genus may have clinical effects depending on the species, or may have no clinical effect, and in some cases worsen the clinical course.

On the other hand, to enhance the function of probiotics, studies on prebiotics, which are food for lactic acid bacteria, are being reported to help beneficial bacteria to settle well in the intestine and to help them grow. In other words, prebiotics are a kind of food for beneficial bacteria to settle and survive in the intestine. Recently, synbiotics, a product that mixes probiotics and prebiotics, have emerged.

The present inventors have completed the present invention by confirming that the pear extract promotes the growth of lactic acid bacteria in view of this point.

The present invention confirms that the pear extract promotes the growth of Lactobacillus acidophilus and Streptococcus thermophilus strains in a medium for beneficial intestinal bacteria, thereby confirming that a free containing pear extract as an active ingredient It is to provide a biotic composition, a synbiotic composition comprising the prebiotic composition, and a functional food composition comprising the synbiotic composition of the above item.

The present invention provides a prebiotic composition comprising a pear extract as an active ingredient.

In addition, the present invention provides a synbiotic composition comprising the prebiotic composition.

In addition, the present invention provides a health functional raw material composition comprising the synbiotic composition.

In addition, the present invention provides a feed composition comprising the synbiotic composition.

According to the present invention, by confirming that the pear extract promotes the growth of Lactobacillus acidophilus and Streptococcus thermophilus strains in the medium for beneficial intestinal bacteria, the pear extract is used as a prebiotic composition can be provided as

1 is a diagram showing a method for preparing a prebiotic composition according to the present invention.
Figure 2 is a graph showing the growth curve over time of the probiotic lactic acid bacteria, Lactobacillus acidophilus ( Lactobacillus acidophilus ) strain when the prebiotic composition according to the present invention is administered.
3 is a graph showing the growth curve over time of a Streptococcus thermophilus strain, a probiotic lactic acid bacterium, when the prebiotic composition according to the present invention is administered.
Figure 4 is a graph comparing the growth curve with time of the Lactobacillus acidophilus strain, a probiotic lactic acid bacterium, with fructooligosaccharide and galactooligosaccharide when the prebiotic composition according to the present invention is administered. am.
5 is a diagram showing the function of a prebiotic composition containing a pear extract.

The terms used in this specification have been selected as currently widely used general terms as possible while considering the functions in the present invention, which may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

Unless defined otherwise, all terms used herein, including technical and scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not

Numerical ranges are inclusive of the values defined in that range. Every maximum numerical limitation given throughout this specification includes all lower numerical limitations as if the lower numerical limitation were expressly written. Every minimum numerical limitation given throughout this specification includes all higher numerical limitations as if the higher numerical limitation were expressly written. Any numerical limitation given throughout this specification shall include all numerical ranges within the broader numerical range, as if the narrower numerical limitation were expressly written.

Hereinafter, the present invention will be described in more detail.

The present invention provides a prebiotic composition comprising a pear extract as an active ingredient.

The pear is a fruit of the pear tree, and contains 10-14% sugar, 140-170 mg of potassium, and 3-6 mg of vitamin C in 100 g of the pulp.

The pear extract is a pear tree ( Pyrus pyrifolia cv. Shingo or Pyrus pyrifolia cv. Hwangkeumbae) may be a fruit containing the peel or a fruit peel extracted with an extraction solvent. The extraction solvent may be 20% to 90% ethanol.

The prebiotics serve as a nutrient source for microorganisms, and the prebiotic composition according to the present invention can promote the growth and proliferation of beneficial intestinal bacteria.

The intestinal beneficial bacteria may be Lactobacillus genus, Streptococcus genus or Bifidos genus strains, specifically, Lactobacillus acidophilus or Streptococcus thermophilus ), but may be limited thereto. no.

In addition, the present invention provides a synbiotic composition comprising the prebiotic composition.

The synbiotic composition may be prepared into capsules, beads, edible solutions, suspensions, powders, granules, tablets, foaming agents, and the like by a conventional method in the art for easy oral administration.

In addition, the present invention provides a functional food composition comprising the synbiotic composition.

The present invention can be generally used as a commonly used food product.

The food composition of the present invention can be used as a health functional food. The term "health functional food" means a food manufactured and processed using raw materials or ingredients useful for the human body in accordance with the Health Functional Food Act, and "functionality" refers to the structure and function of the human body. It refers to ingestion for the purpose of obtaining useful effects for health purposes such as regulating nutrients or physiological effects.

The food composition of the present invention may contain conventional food additives, and whether or not it is suitable as the "food additive" is determined according to the general rules and general test methods of food additives approved by the Ministry of Food and Drug Safety, unless otherwise specified. It is judged according to the standards and standards related to the item.

The items listed in the “Food Additives Code” include, for example, chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid; Mixed preparations such as sodium L-glutamate preparation, noodle-added alkali agent, preservative agent, and tar color agent can be mentioned.

The food composition of the present invention may be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, and the like.

For example, among the health functional foods in the form of capsules, hard capsules can be prepared by mixing and filling a conventional hard capsule with the composition according to the present invention with additives such as excipients, and the soft capsule contains the composition according to the present invention. It can be prepared by mixing with additives such as excipients and filling in capsule bases such as gelatin. The soft capsule formulation may contain a plasticizer such as glycerin or sorbitol, a colorant, a preservative, and the like, if necessary.

The term definitions for the excipients, binders, disintegrants, lubricants, flavoring agents, and the like are described in documents known in the art and include those having the same or similar functions. There is no particular limitation on the type of food, and includes all health functional foods in the ordinary sense.

In addition, the present invention provides a feed composition comprising the synbiotic composition.

The present invention provides a feed composition comprising synbiotics mixed with probiotics as well as prebiotics for promoting the growth of lactic acid bacteria in order to improve the immune function of animals and reduce side effects such as mycosis caused by the administration of antibiotics to the feed.

The feed means any natural or artificial diet, meal meal, etc., or a component of the meal meal, intended for or suitable for ingestion and digestion by livestock. The feed may include a feed additive or a supplementary feed.

The type of feed is not particularly limited, and feed commonly used in the art may be used. Non-limiting examples of the feed include plant feeds such as grains, root fruits, food processing by-products, algae, fibers, pharmaceutical by-products, oils and fats, starches, gourd or grain by-products: proteins, inorganics, and animal feeds such as oils and fats, minerals, oils and fats, unicellular proteins, zooplankton, or food. These may be used alone or may be used in combination of two or more.

Hereinafter, examples and experimental examples will be described in detail to help the understanding of the present invention. However, the following Examples and Experimental Examples are merely illustrative of the content of the present invention, and the scope of the present invention is not limited to the following Examples and Experimental Examples. Examples and experimental examples of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

Example 1: Preparation of prebiotic composition 1 (alcohol immersion extraction method)

As shown in FIG. 1, the prebiotic composition of the present invention was used by extracting Korean golden pears (Pyrus pyrifolia cv. Whanggeumbae). After washing golden pears in a state containing the skins 3 times with purified water, cutting them into 6 to 8 pieces, removing the core, and squeezing them with a green juicer (Hurom Celeb, Hurom Co., Ltd., Gimhae-si, Gyeongnam) to separate the pear skins and pear juice Pear skins, pear juice, and mixtures of pear hulls and pear juice were prepared in liquid or granular form, respectively, as follows.

(1) The pear skin was immersed in 30% ethanol, extracted for 3 days at room temperature, and filtered through a 55 μm bag filter to obtain a filtrate. The obtained filtrate was concentrated with 14.1 brix at 80°C and 1200 rpm (liquid product).

In order to prepare granules, the concentrate solids and dextrin were mixed, but dextrin was added so as not to exceed 70% of the total mixture. After spray-drying the mixture at 180°C - 200°C for 30 seconds, a final granular sample was obtained at 95°C (granular product).

A schematic diagram of the method of Example 1 is shown in FIG. 1 .

Example 2: Preparation of prebiotic composition 2 (alcohol immersion extraction method for mass production)

The preparation method of the prebiotic composition of the present invention provides a method with enhanced concentration in order to facilitate mass production and ingestion in a small amount by modifying method (1) of Example 1.

The prebiotic composition of the present invention was used by extracting Korean pear (Pyrus pyrifolia cv. Shingo). After washing the Shingo pear in a state containing the skin 3 times with purified water, cut it into 6 to 8 pieces, remove the core, and squeeze the pear skin and pear juice with a juicer (fruit and vegetable screw, Dongyang Food Machinery Co., Ltd., Yangsan City, Gyeongnam) separated.

The pear skin was immersed in 30% ethanol, extracted for 3 days at room temperature, and filtered through a 55 μm bag filter to obtain a filtrate. The obtained filtrate was concentrated to 59 brix at 80° C. and 1200 rpm at 9 brix.

Comparative Example 1: Preparation of prebiotic composition 3 (ultra-high pressure extraction)

After washing the golden pear in the state containing the skin 3 times with purified water, cutting it into 6 to 8 pieces, putting it in a plastic bag for vacuum packaging, vacuum packaging it with a vacuum packaging machine (SaengQ, Co., Ltd.), and then vacuum packaging it with an ultra-high pressure machine at 400 MPa After grinding at a pressure of (4000 bar) or 500 MPa (5000 bar), it was separated into pear juice and pear skins with a green juicer (Hurom Celeb, Hurom, Gimhae-si, Gyeongnam).

The pulverized pear skin was immersed in 30% ethanol, extracted for 3 days at room temperature, and filtered through a 55 μm bag filter to obtain a filtrate. After concentration with a rotary evaporator, it was dissolved in 60% ethanol.

Comparative Example 2: Preparation of prebiotic composition 4 (supercritical extraction)

After washing the golden pear in a state containing the skin 3 times with purified water, cut into 6 to 8 pieces, and freeze-dried. The dry solid was extracted with CO2 at 350 bar and 50° C. for 60 minutes, and then 5% ethanol was added and extracted for 120 minutes. After concentration with a rotary evaporator, it was dissolved in 60% ethanol.

Experimental Example 1: Effect of pear extract on growth of probiotic lactic acid bacteria

In order to evaluate the effect of the pear extract of Examples on the proliferation of beneficial intestinal bacteria as a prebiotic, probiotic lactic acid bacteria Lactobacillus acidophilus ( Lactobacillus acidophilus ) and Streptococcus thermophilus ( Streptococcus thermophilus ) On the growth of The effect was confirmed.

Lactobacillus acidophilus ( Lactobacillus acidophilus ) or Streptococcus thermophilus strains were cultured in MRS medium, and 1 mg/mL of pear extract was added to the experimental group medium. In the other control group, only 60% ethanol was added to the MRS medium.

In each medium, the strain was inoculated to have an absorbance (optical Density) of 0.2 at 600 nm, Lactobacillus at 37 ° C, and Streptococcus thermophilus at 42 ° C. OD600 was measured at 5-hour intervals and graphed as a growth curve of each strain ( FIGS. 2 and 3 ).

As shown in Figures 2 and 3, in the medium to which the pear extract of Example 1 was added, both Lactobacillus acidophilus and Streptococcus thermophilus strains showed significant growth curves compared to the control groups. appeared to have grown significantly.

The above results demonstrated that the pear extract of Example 1 has an effect of promoting the growth of probiotic strains, which are beneficial intestinal bacteria, and these results suggest that the pear extract according to the present invention can be used as a prebiotic.

Experimental Example 2: Comparison of the effect of pear extract and prebiotic oligosaccharides on growth of probiotic lactic acid bacteria

In order to compare and evaluate the effect of the pear extract of Example on the proliferation of beneficial intestinal bacteria as a prebiotic with fructooligosaccharide and galactooligosaccharide, which are representative prebiotics, the probiotic lactic acid bacteria Lactobacillus acidophilus ( Lactobacillus acidophilus ) was confirmed to have an effect on the growth of

Lactobacillus acidophilus ( Lactobacillus acidophilus ) The strain was cultured in MRS medium, and 3 mg/mL of pear extract, fructooligosaccharide or galactooligosaccharide was added to the experimental group medium.

In each medium, the strain was inoculated to have an absorbance (optical Density) of 0.2 at 600 nm, and Lactobacillus was stationary cultured at 37°C. OD600 was measured at 5-hour intervals and graphed as a growth curve of each strain (FIG. 4).

As shown in Figure 4, the growth curve of the Lactobacillus acidophilus strain in the medium to which the pear extract of Example 2 was added was almost the same as the growth curve in the medium to which fructooligosaccharide or galactooligosaccharide was added. appeared to grow equally.

The above results show that the pear extract of Example 2 is almost equivalent to fructooligosaccharide and galactooligosaccharide, which are products approved by the Ministry of Food and Drug Safety in terms of intestinal health function as prebiotics that promote the growth of probiotic strains, which are beneficial intestinal bacteria. The pear extract according to the present invention contains various anti-allergic ingredients, hangover detoxification ingredients, etc. including polyphenol antioxidants such as arbutin and chlorogenic acid, which are described below, unlike the above sugars (Fig. 5). It suggests that it can be used as a prebiotic that is superior to oligosaccharides and galactooligosaccharides in health functional areas.

As the specific parts of the present invention have been described in detail above, for those of ordinary skill in the art, it is clear that these specific descriptions are only preferred embodiments, and the scope of the present invention is not limited thereby. do. That is, the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (10)

A prebiotic composition comprising a pear extract as an active ingredient. The prebiotic composition according to claim 1, wherein the pear extract is obtained by extracting the fruit peel of a pear tree (Pyrus pyrifolia ) with an extraction solvent. The prebiotic composition according to claim 1, wherein the prebiotic composition promotes the growth of beneficial intestinal bacteria. The prebiotic composition according to claim 3, wherein the beneficial bacteria in the intestine is a strain of Lactobacillus, Streptococcus, or Bifidobacterium. A synbiotic composition comprising the prebiotic composition of any one of claims 1 to 4. The synbiotic composition according to claim 5, wherein the synbiotic composition contains beneficial intestinal bacteria. The synbiotic composition according to claim 6, wherein the beneficial bacteria in the intestine is a strain of Lactobacillus, Streptococcus, or Bifidobacterium. The synbiotic composition according to claim 5, wherein the synbiotic composition is prepared in the form of granules. A functional food composition comprising the synbiotic composition of any one of claims 5 to 8. A feed composition comprising the synbiotic composition of any one of claims 5 to 8.

Images