KR20210111637A - Composition comprising pearl extract, prepararing method and application thereof - Google Patents

Abstract

The present invention relates to: a method for manufacturing a composition containing an extract of nacre which obtains the extract of nacre and provides a hair cosmetic using the same as an active ingredient to prevent hair damage from the outside and help to improve damaged hair by improving the volume, elasticity, gloss, and smoothness of the hair; and cosmetics for hair comprising the composition comprising the extract of nacre obtained by the method as the active ingredient. For the same, the present invention comprises: (a) preparation step to prepare a pearl powder; (b) an alkali solubilization step; (c) a stabilization step to prevent precipitation of high molecular peptides greater than oligomer; (d) concentration step of solubilization using clathrate hydrate; (e) a filtration and separation step of separating the hydrate and the remaining solubilized product forming the clathrate hydrate; (f) an obtaining step to obtain the pearl protein solubilized product isolated in step (e); (g) a step of dry pulverizing Pachymeniopsis elliptica, a kind of red algae, to prepare a powder; (h) a step of performing hydrolysis for 1 to 3 hours at 1 atm under an acid catalyst in a range of 80℃ or higher and 100℃ or lower; (i) a step of removing unreacted residues remaining in the hydrolyzed product in step (h); (j) a step of adding and mixing the inorganic salt catalyst within the range of 80 to 100% of the amount of the acid catalyst added in step (h) to neutralize the pH within the range of 5 to 9; (k) a step of concentrating the hydrolysis neutralized product neutralized in step (j) within the range of 5 to 30 Brix; and (l) a step of obtaining a Pachymeniopsis elliptica extract after the step (k).

Description

A composition comprising an extract of nacres, and a method and use thereof for manufacturing the same

The present invention relates to a composition comprising an extract of nacres and a method for preparing and using the same. More specifically, by providing a hair cosmetic using the extract of nacre as an active ingredient, it prevents hair damage from the outside and improves the volume, elasticity, gloss and smoothness of the hair to help improve damaged hair. It relates to a method for producing a composition comprising an extract of nacres, and to a cosmetic for hair using a composition comprising an extract of nacre obtained by the method as an active ingredient.

Hair is characterized by being easily damaged by the external environment. Recently, the hair of modern people is severely damaged due to frequent use of dyes, perms, excessive use of dryers, and exposure to ultraviolet rays, and the hair is dry and brittle, reducing its shine, softness, and smoothness, making styling difficult.

Accordingly, hair products that can prevent hair damage, increase gloss, smoothness, ease of combing, volume, or loss of strength in hair cosmetics such as hair serum and water essence are continuously being marketed, and expectations for their effects are high. is increasing

Conventional hair products can enhance the above effects by using a conditioning agent such as a combination of silicone, hydrocarbon oil, fatty ester, and the like, but these ingredients have the disadvantage of causing wetness and stickiness to the hair.

In addition, in order to improve the conditioning of the hair, hydrolyzed conchiolin extract or soybean protein is used to develop hair products that can improve the moisture, gloss, elasticity, and softness of the hair. There is a limit to returning the hair shiny and soft.

KR Registered Patent Publication No. 1337892 (Registered on Dec. 2013)

The present invention has been devised to solve the above problems, and in particular, pearl protein solubles that can help to improve damaged hair by preventing hair damage from the outside and improving hair volume, elasticity, gloss and smoothness An object of the present invention is to provide a method for obtaining a cargo and gambling extract, and a cosmetic for hair using the pearl protein solubilized product and gambling extract obtained by the method as active ingredients.

A method for obtaining pearl protein solubilized product and gambling extract according to the present invention devised to achieve the above object, and a cosmetic for hair containing pearl protein solubilized product and gambling extract obtained by the method as an active ingredient (a) pearl powder preparatory stage of preparation; (b) Complete solubilization by mixing the pearl powder prepared in step (a) with an alkali agent having a mass ratio of 20% or more of the protein content in the pearl powder and heating and stirring at a temperature of 100 or more for 2 hours or more to hydrolyze to the constituent units of peptides and amino acids Alkali solubilization step to perform; (c) adding an acidic aqueous solution containing hydrochloric acid, sulfuric acid, acetic acid, and citric acid to the solubilized product reacted in step (b) to neutralize it to a pH range of 8 or more and 9 or less, stabilizing step to prevent precipitation of oligomeric or higher polymer peptides ; (d) The solubilized material and mineral water carried out in step (c) are put into a high-pressure reactor in an environment in which carbon dioxide is present, and concentrated at 30 atmospheres under the conditions of 0 or more and 4 or less. By maintaining the form in which the gas containing carbon dioxide is captured in the high-pressure reactor, the base nitrogen is removed as the moisture of the solubilized material is removed as much as the degree of hydration of the gas composed of carbon dioxide and volatile basic nitrogen injected into the high-pressure reactor. concentration of the solubilized product; (e) a filtration and separation step of transferring the solubilized product from which the base nitrogen has been removed in step (d) to a separator to separate the hydrate and the remaining solubilized product to form a clathrate hydrate; (f) obtaining a pearl protein solubilized product isolated in step (e); (g) dry pulverizing gambling, which is a kind of red algae, to prepare a powder; (h) performing hydrolysis for 1 to 3 hours at 80-100° C. at 1 atm pressure under an acid catalyst containing 10-30% citric acid based on the weight of the raw material; (i) removing unreacted residues remaining in the product hydrolyzed in step (h); (j) the product hydrolyzed under the acid catalyst of step (h) and the inorganic salt catalyst including sodium carbonate, sodium bicarbonate, sodium hydroxide and potassium hydroxide in the range of 80 to 100% of the amount of the acid catalyst added in step (h) Neutralizing the pH within the range of 5 to 9 by addition and mixing; (k) concentrating the hydrolysis-neutralized product neutralized in step (j) within the range of 5 to 30Brix; and (l) after step (k), obtaining a gambling extract.

In addition, (m) may further include the step of mixing the pearl protein solubilized product resulting from step (f) and the gambling extract resulting from step (l).

In addition, it includes a cosmetic for hair containing the pearl protein solubilized product and gambling extract obtained by the above method as active ingredients, and the cosmetic for hair may be characterized in that it is a shampoo, tonic, or treatment.

According to the present invention, there is provided a method for obtaining a pearl protein solubilized product and a gambling extract, and a hair cosmetic containing the pearl protein solubilized product and a gambling extract obtained by the method, thereby preventing hair damage from the outside and increasing the volume of hair. It is effective in helping to improve damaged hair by improving elasticity, luster and smoothness.

1 is a process flow chart of a method for obtaining pearl protein solubilized product and gambling extract according to a preferred embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. First, in adding reference numerals to the components of each drawing, it should be noted that the same components are given the same reference numerals as much as possible even though they are indicated on different drawings. In addition, in describing the present invention, if it is determined that a detailed description of a related known configuration or function may obscure the gist of the present invention, the detailed description thereof will be omitted. In addition, preferred embodiments of the present invention will be described below, but the technical spirit of the present invention is not limited thereto and may be variously implemented by those skilled in the art without being limited thereto.

1 is a process flow chart of a method for obtaining pearl protein solubilized product and gambling extract according to a preferred embodiment of the present invention.

1, the method for obtaining pearl protein solubilized product and gambling extract according to a preferred embodiment of the present invention includes pearl powder preparation step (S10), alkali solubilization step (S20), stabilization step (S30), clathrate hydrate Concentration step (S40), filtration and separation step (S50), pearl protein solubilization step (S60), gambling powder preparation step (S70), hydrolysis step (S80), filtration step (S90), It is configured to include a neutralization step (S100), a gambling extract obtaining step (S110), and a mixing step (S120).

Hereinafter, the pearl powder preparation step (S10) will be described.

The pearl powder preparation step (S10) is a step of preparing the pearl powder.

Since pearls contain conchiolin components such as amino acids and other organic substances, they have an excellent effect in whitening darkened pigments such as spots. In addition, if you eat and apply pearls, your skin will become lustrous and moist, and it will prevent skin aging and contain ingredients that are good for age spots, freckles, pimples, acne, and skin boils.

These pearls are crushed and prepared into powder.

In the alkali solubilization step (S20), the pearl powder prepared in the pearl powder preparation step (S10) and an alkali agent in a mass ratio of 20% or more of the protein content contained in the pearl powder are mixed and heated and stirred at a temperature of 100 or more for 2 hours or more to form peptides and amino acids Complete solubilization is carried out, hydrolyzed to the unit.

More specifically, the alkali agent mixed in the alkali solubilization step (S20) is characterized by using a weak alkali or strong alkali including calcium carbonate, sodium carbonate, sodium bicarbonate, sodium hydroxide, potassium hydroxide.

In addition, if an alkali agent of 20% or less of the protein content contained in the pearl powder is used in the alkali solubilization step (S20), it leads to incomplete solubilization, and complete solubilization does not occur.

Here, complete solubilization means that the protein is hydrolyzed to the peptide and amino acid constituent units, and incomplete solubilization means that the protein is not hydrolyzed to the peptide and amino acid constituent units.

In addition, in the alkali solubilization step (S20), it is preferable to boil it with carbon dioxide and volatile basic nitrogen injected into the high-pressure reactor by maintaining the form in which the gas under heating and stirring at a temperature of at least 80 ° C., mainly 100 ° C. is collected, The minimum reaction temperature required for complete hydrolysis of do.

In addition, in the alkali solubilization step (S20), it is preferable to heat and stir at a temperature of 100 ° C. or more for 2 hours or more, since this is the minimum reaction time required for complete hydrolysis of protein.

In the stabilization step (S30), an acidic aqueous solution containing hydrochloric acid, sulfuric acid, acetic acid, and citric acid is added to the solubilized product reacted in the alkali solubilization step (S20) to neutralize it to a pH range of 8 or more and 9 or less to precipitate an oligomeric or higher polymer peptide make sure it doesn't happen

At this time, in the stabilization step (S30), neutralizing to a pH of 8 or more and pH 9 or less by adding an acidic aqueous solution In a neutral or acidic range of pH 7 or less, polymeric peptides of oligomeric or more that have not been fully reacted may be precipitated out in the aqueous solution, It cannot be used as a cosmetic material (cosmetics) in the range of pH greater than 9.

In the concentration step (S40) of the solubilization using clathrate hydrate, the solubilized material and the mineral water performed in the stabilization step (S30) are put into a high-pressure reactor in an environment in which carbon dioxide is present, and concentrated under the conditions of 30 atmospheres and 4 or less. , as the water of the solubilized material is removed as much as the degree of hydration for the gas containing carbon dioxide in the lattice structure of the mixture of the solubilized material and the mineral water, the basic nitrogen remaining in the ammonia odor is removed.

Hydrate is the trapping of low-molecular gases in a space where water molecules are formed by hydrogen bonding, in which gas containing carbon dioxide is captured.

In addition, in concentration using clathrate hydrate, the number of hydration required for hydrate crystallization depending on the type of object molecule because water, the main molecule, surrounds the gas, which is the object molecule, to form a hydrate crystal by controlling the composition of a specific gas and liquid. is determined, and at this time, only pure water is consumed and combines with gas molecules to form a hydrate, so it is possible to separate from the solution, including the gaseous substances present in the raw material solution, and as much as the amount of water consumed to form the hydrate The concentration of the solution is increased so that the concentration can be controlled by controlling the amount of water consumed.

In the present invention, clathrate hydrate was used, and the ammonia odor generated during hydrolysis of pearl protein by consuming pure water forms a hydrate by combining residual basic nitrogen with pure water, mineral water. By making it possible to remove and concentrate, it is possible to easily remove ammonia odor of basic nitrogen caused by hydrolysis of pearl protein, which is a conventional problem, and to separate and recover only concentrated high-concentration pearl protein solubilized product, thereby increasing productivity and economy by increasing processing yield. improvement, of course.

In particular, the reaction conditions for performing hydrate so that the gas containing carbon dioxide is captured in the mixture of the solubilized material and mineral water injected into the high-pressure reactor in the concentration step (S40) of the solubilization using clathrate hydrate is 30 atm, 0 or more 4 or less are preferable.

This is a phase equilibrium condition for forming a hydrate, and when it is 30 atm, when it is more than 4, hydrate formation does not occur, and when it is 30 atm, particularly, when it is less than 0, all of the reactants form a hydrate, thereby eliminating volatile base nitrogen and concentrating the solubilized product. This is because it is difficult to separate the interface for

In addition, the reaction time of carbon dioxide hydrate in the concentration step (S40) of the solubilized product using the clathrate hydrate is preferably concentrated for 30 minutes or more and 1 hour or less.

This is the induction time, which is determined by the time to reach phase equilibrium of the gas hydrate. If the reaction time is less than 30 minutes or more than 1 hour, it is not suitable for the time to reach phase equilibrium, so ammonia odor through clathrate hydrate is a residual volatile base. This is because the interfacial separation for the exclusion of nitrogen and concentration of the solubilized material is difficult.

In the filtration and separation step (S50), the solubilized product from which the base nitrogen has been removed in the concentration step (S40) of the solubilized product using clathrate hydrate is passed through a filter including a micro filter in the high-pressure reactor and transferred to the separator to obtain the clathrate hydrate It is a step of separating the hydrate that formed and the residual solubilized product from which the base nitrogen has been removed.

The pearl protein solubilized product obtaining step (S60) is a step of obtaining a solubilized product from which volatile base nitrogen is removed while passing through the filter and separator in the filtration and separation step (S50).

The concentrated pearl protein solubilized product thus obtained is used as a cosmetic material (cosmetics).

Gambling powder preparation step (S70) is a step of preparing for gambling, which is a kind of red algae.

Gambling is a seaweed of the red algae Jinuarit family, 15-40 cm high, leaf-shaped, and divided into several branches. The flesh is reddish-purple, yellow, and green, and turns purple when dried. It is used as a material for making grass together with sagebrush or as a material for making adhesives. It is clustered in crevices of rocks along the coast and is distributed in Korea and Japan.

Gambling, a red type, contains L-methionine, a type of α-amino acid containing sulfur. L-methionine is a sulfur-containing amino acid (amino acid containing sulfur (S) in molecules such as methionine, cystine, and homocysteine) constituting a protein. Although its content in protein is small, it acts as a methyl group donor in vivo. In addition, L-methionine is an essential amino acid absolutely necessary for the growth of animals, and since it is a precursor of cystine, a hair constituent material, it is a substance that can help hair growth.

As described above, gamble, which is effective for hair growth, is prepared by drying and pulverizing it into a powder.

The hydrolysis step (S80) is performed using an acid catalyst of 10 to 30% of the total weight of the gambling powder and gambling prepared in the gambling powder preparation step (S70) at 1 atm, 80 ° C to 100 ° C for 1 hour or more and 3 hours or less hydrolysis is carried out with

If the content of the acid catalyst in the hydrolysis step (S80) is less than 10%, it may accompany a part of the hydration reaction of alginic acid contained in gambling, which may cause clogging in the next step, the filtration step (S90), and the acid catalyst content If this is 30% or more, the effect on the hardening reaction of alginic acid contained in gambling is not significantly different from the concentration below 30%.

Alginic acid is an acidic polysaccharide with excellent water-retaining ability (capacity to hold water). It has a high degree of hydration to water, so it swells after a hydration reaction with a large amount of water, and viscous gelation occurs in the next step, filtration step (S90). Since the pores of the filter can be blocked, the separation process is preferably performed in the hydrolysis step (S80).

In addition, if the reaction temperature in the hydrolysis step (S80) is less than 80 ° C, the rate of diffusion of water into the gambling is slow, the hydrolysis reaction rate is reduced. When it exceeds 100 degrees Celsius, the dielectric constant of water is low, and the ion exchange capacity of the acid catalyst is reduced, thereby reducing the hydrolysis reaction rate.

In addition, the acid catalyst used in the hydrolysis step (S80) may use citric acid or the like, and the type of the acid catalyst is not limited to a specific one.

In addition, if the reaction time in the hydrolysis step (S80) is less than 1 hour, the hydrolysis progress may be low and the hydrolysis yield may be reduced. As it progresses, there is a risk of generation of peroxides or carbides.

The filtering step (S90) is a step of removing unreacted residues remaining in the hydrolyzate. Here, the unreacted residue is mainly ash contained in the raw material that does not show hydrolysis reactivity, and may include other hydrolyzed fibers, proteins, lipids, and the like that have not been completed.

The neutralization step (S100) is a step of adding and mixing an inorganic salt catalyst to the organic acid catalyst hydrolysis reaction product within the range of 80 to 100% of the amount of the acid catalyst added in the hydrolysis step (S80) to neutralize in a range of pH 5 or more and pH 9 or less. am.

In addition, the inorganic salt catalyst used in the neutralization step (S100) may use any one of sodium carbonate, sodium bicarbonate, sodium hydroxide, and potassium hydroxide, wherein the type of the inorganic salt catalyst is not limited to a specific one.

When the amount of the inorganic salt catalyst added is less than 80%, the neutralization value has a hydrogen ion concentration of less than pH 5, so it may cause skin irritation when using cosmetics. Therefore, it may cause percutaneous irritation when using cosmetics.

The concentration step (S110) is a step of controlling the concentration within the range of 5 brix to 30 Brix by concentrating the hydrolysis neutralized product. More preferably, in the concentration step (S110), the hydrolysis-neutralized product is concentrated to 20Brix using a vacuum and a reduced pressure concentrator to obtain 100 ml of a concentrate.

In the concentration step (S110), if the concentration of the hydrolyzed neutralized product is 5Brix, it is the normal concentration level of the initial hydrolyzed neutralized product, and if it exceeds 30Brix, it may cause recrystallization due to supersaturation of the solubility of saccharides in the concentrate, and the present invention Although not mentioned in , the content of solubilizer and ethanol increases in the cosmetic formulation step to be carried out later, which may limit the scope of formulation prescription.

Gambling extract obtaining step (S110) is a step of obtaining a concentrated gambling extract using a vacuum and reduced pressure concentrator in the concentration step (S110).

[Example 1] Gambling extract obtaining method

Gambling powder is prepared by dry grinding 100 g of gambling powder, 30 g of citric acid is dissolved in 1 L of purified water, and hydrolyzed at 100 ° C for 1 hour to separate the hydrolyzate and residue (ash fraction) by filtration to obtain 400 ml of hydrolyzate. was obtained, and the concentration of the hydrolyzate at this time was about 5Brix. Next, 24 g of sodium bicarbonate was mixed and neutralized with the obtained hydrolyzate to obtain 400 ml of a neutralized product. At this time, the hydrogen ion concentration of the hydrolysis-neutralized product is pH 5. Next, the hydrolysis-neutralized product was concentrated to 20 Brix using a vacuum concentrator to obtain 100 ml of a concentrate.

The mixing step (S120) mixes the pearl protein solubilized product resulting from the step S60 and the gambling extract resulting from the step S110.

The mixed pearl protein solubilized product and gambling extract are used as cosmetic ingredients (cosmetics).

The cosmetic for hair containing the pearl protein solubilized product and the gambling extract obtained by the method for obtaining the pearl protein extract and gambling extract according to a preferred embodiment of the present invention as an active ingredient is a shampoo, tonic, or treatment, characterized in that: In the description, the composition of the shampoo, toner, and treatment will be described in detail.

I. Shampoo composition containing pearl protein extract and gambling extract as active ingredients

The shampoo composition of cosmetics for hair is polyquaternium-10, sodium laureth(3)sulfite, decylglucoside, cocaamidopropylbetaine, DL-panthenol, polyquaternium-7, pH adjuster, paraoxybenzoate ester, coca It consists of mide DEA, aroma oil, combination fragrance, pearl protein soluble product, and gambling hydrolyzate.

More specifically, polyquaternium-10 0.3% by weight, sodium laureth (3) sulfite 34% by weight, decylglucoside 5% by weight, cocaamidopropylbetaine 5% by weight, DL-panthenol 0.2% by weight, polyquater nium-7 0.4% by weight, pH adjuster 0.15%, paraoxybenzoic acid ester 0.2% by weight, cocamide DEA 2.5%, aromatic oil 0.5% by weight, combination fragrance 2% by weight, pearl protein solubilizer 35.75% by weight, gambling hydrolyzate 5 It is characterized in that it consists of weight %.

II. Tonic composition comprising pearl protein extract and gambling extract as active ingredients

Among hair cosmetics, the tonic composition consists of l-menthol, salicylic acid, tocopherol acetate, HCO60, DL-panthenol, pH adjuster, ethanol, aroma oil, blended fragrance, pearl protein solubilized product, and gambling hydrolyzate.

More specifically, 1-menthol 0.1% by weight, salicylic acid 0.2%, tocopherol acetate 0.03% by weight, HCO60 0.1% by weight, DL-panthenol 0.2% by weight, pH adjuster 0.15% by weight, ethanol 35% by weight, aroma oil 0.5% by weight, It is characterized in that it is composed of 0.03% by weight of a combination fragrance, 58.69% by weight of pearl protein solubilized product, and 5% by weight of gambling hydrolyzate.

Ⅲ. Treatment composition comprising pearl protein extract and gambling extract as active ingredients

Among hair cosmetics, treatment compositions include glycerin, hydrolyzed keratin, arginine/lysine polypeptide, hyaluronic acid, dipropylene glycol, pH adjuster, phenoxyethanol, aroma oil, HCO60, dimethicone, pearl protein It consists of solubilized cargo and gambling hydrolyzate.

More specifically, glycerin 5% by weight, hydrolyzed keratin 1% by weight, arginine/lysine polypeptide 1% by weight, hyaluronic acid 2% by weight, dipropylene glycol 2% by weight, pH adjuster 0.15% by weight , 0.2% by weight of phenoxyethanol, 0.5% by weight of aroma oil, 0.1% by weight of HCO60, 5% by weight of dimethicone, 79.05% by weight of pearl protein solubilization, and 5% by weight of gambling hydrolyzate.

Among the compositions contained in shampoos, tonics, and treatments using the pearl protein extract and gambling extract as active ingredients, the combined fragrance is a substance administered to increase the lasting power of the fragrance. It can be obtained by mixing spices. Here, the type of the combination fragrance is not limited to a specific one.

The above description is merely illustrative of the technical idea of the present invention, and those of ordinary skill in the art to which the present invention pertains may make various modifications, changes and substitutions within the scope without departing from the essential characteristics of the present invention. will be. Accordingly, the embodiments disclosed in the present invention and the accompanying drawings are intended to explain, not to limit the technical spirit of the present invention, and the scope of the technical spirit of the present invention is not limited by these embodiments and the accompanying drawings. . The protection scope of the present invention should be construed by the following claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

S10 - Pearl Powder Preparation Step
S20 - alkali solubilization step
S30 - stabilization phase
S40 - Concentration step of solubilization using clathrate hydrate
S50 - Filtration and separation steps
S60 - Step of obtaining pearl protein solubilizer
S70 - Gambling Powder Preparation Stage
S80 - hydrolysis step
S90 - Filtration Stage
S100 - Neutralization Phase
S110 - Gambling Extract Obtaining Step
S120 - mixing stage

Claims (2)

(a) a preparation step of preparing pearl powder;
(b) mixing the pearl powder prepared in the preparation step with an alkali agent having a mass ratio of 20% or more of the protein content in the pearl powder and heating and stirring at a temperature of 100 ° C. or higher for 2 hours or more to hydrolyze to the constituent units of peptides and amino acids an alkali solubilization step in which basic nitrogen is generated during the hydrolysis;
(c) adding an acidic aqueous solution containing hydrochloric acid, sulfuric acid, acetic acid, and citric acid to the solubilized product reacted in the alkali solubilization step to neutralize it to a pH range of 8 or more and 9 or less, stabilizing step to prevent precipitation of oligomeric or higher polymer peptides;
(d) In the stabilization step, in order to remove the base nitrogen generated from the solubilized product, the solubilized product and water are put into a high-pressure reactor in an environment with carbon dioxide and concentrated under the conditions of 30 atmospheres and 0°C or higher and 4°C or lower. Solubilizer and water form a lattice structure, hydrate is performed in which gas containing carbon dioxide in the lattice structure is collected, and water surrounds the gas containing carbon dioxide and basic nitrogen to form a hydrate, so that the basic nitrogen contained in the solubilizer is Concentrating the solubilized product using clathrate hydrate to separate;
(e) filtration and separation step of transferring the concentrated solubilizer to a separator in the concentration step of the solubilization using clathrate hydrate to separate the hydrate and the base nitrogen from which the hydrate is formed to form a clathrate hydrate;
(f) obtaining a pearl solubilized product isolated in the filtration and separation steps;
(g) dry pulverizing a type of red algae, grateloupia elliptica, to prepare a powder;
(h) performing hydrolysis for 1 to 3 hours in the range of 1 atm, 80 ℃ or more and 100 ℃ or less under an acid catalyst containing 10 to 30% of citric acid based on the weight of gambling powder;
(i) removing unreacted residues remaining in the hydrolyzed product in step (h);
(j) 80% by weight to 100% by weight of the acid catalyst added in step (h) of the resultant hydrolyzed under the acid catalyst of step (h) and an inorganic salt catalyst comprising sodium carbonate, sodium bicarbonate, sodium hydroxide, and potassium hydroxide Neutralizing the pH within the range of 5 to 9 by addition and mixing within the % range;
(k) concentrating the hydrolysis neutralized product neutralized in step (j) within the range of 5 to 30Brix; and
(l) after step (k), obtaining a gambling extract
A method of obtaining a pearl protein solubilized product and gambling extract, comprising a. According to claim 1,
(m) (f) a method of obtaining pearl protein solubilized product and gambling extract, further comprising the step of mixing the pearl protein solubilized product resulting from step (l) and the gambling extract resulting from step (l).

Images