KR20200097673A - Composition for improving skin troubles comprising mortierella oil as an active ingredient - Google Patents

Abstract

The present invention relates to a composition for alleviating skin troubles containing oil extracted from Mortierella Isabellina culture as an active ingredient. More particularly, the present invention relates to a cosmetic composition, a health functional food composition, or a quasi-drug showing excellent effects in alleviating skin troubles.

Description

Composition for improving skin troubles comprising mortierella oil as an active ingredient}

The present invention relates to a cosmetic composition containing Mortierella oil, and more particularly, containing oil extracted from Mortierella isabellina culture solution, excellent in enhancing skin elasticity, improving wrinkles, or improving skin troubles. It relates to a cosmetic composition having an effect. In addition, the present invention relates to a health functional food composition and a quasi-drug composition comprising an oil extracted from Mortierella Isabelina culture.

Collagen is a major matrix protein produced by fibroblasts in the skin, and exists in the extracellular interstitial. Its important functions include skin mechanical firmness, connective tissue resistance and tissue bonding, cell adhesion support, and cells. The induction of division and differentiation (when the organism grows or heals wounds) is known. Such collagen is reduced by age and photoaging due to ultraviolet irradiation, and collagen reduction is promoted by the activity of collagenase enzymes that degrade collagen. This is known to be closely related to the formation of wrinkles in the skin. In addition, collagen fibers are matrix proteins that play an important role in retaining moisture in the dermal layer, and these matrix proteins adsorb moisture and increase moisture retention in the structures they form, allowing the skin to maintain a state containing adequate moisture. Through this, it is known to be involved in maintaining the elasticity of the skin.

On the other hand, elastin fibers form cross-links with collagen and are an important skin component for wrinkle formation, which is involved in skin elasticity. Deficiency and aggregation of elastin fibers, and a marked increase in the activity of elastase, an elastin-degrading enzyme, have been found to be one of the factors of skin wrinkles. Elastase is the only enzyme that can break down elastin, and its inhibition is known to fundamentally reduce skin wrinkles.

In addition, collagen and elastin fibers are matrix proteins that play an important role in water retention in the dermal layer, and these matrix proteins adsorb moisture and increase moisture retention within the structures that make up them so that the skin can maintain a state containing adequate moisture. It is known to be involved in maintaining the elasticity of the skin through this.

Currently, retinoids, adenosine, animal placenta-derived proteins, and chlorella extracts are known as cosmetics for improving skin elasticity and wrinkles. The best known retinol is a substance that promotes collagen synthesis and inhibits elastase enzymes, but it is unstable. When applied to the skin, the amount of use is limited due to safety issues such as irritation and redness. Chlorella extract, etc., are insignificant and practically It is known that it is difficult to expect the effect of improving elasticity and wrinkles.

The inflammatory response is manifested by five phenomena: feeling of redness, pricking feeling, burning hotness, swelling, and changes in tissue. It is a physiological reaction to protect a living body from invasion of substances and mechanical damage. This inflammatory phenomenon results in a large increase of various types of polymorphonuclear leukocytes (PMNs) and immune substances, and these increased cells secrete various types of proteolytic enzymes and cytokines, which are inflammatory cell products, to provide treatment and defense. Allows you to However, this action may cause harmful damage to adjacent tissue cells and non-cellular components, and oxidative stress such as ultraviolet rays, free radicals, etc. activate inflammatory factors, causing various diseases and skin aging. . Therefore, under appropriate conditions, normal function is restored after the initial state of inflammation has passed, but if the irritant that stimulates inflammation does not disappear or is made continuously, as a result, chronic inflammation occurs, leading to more serious tissue damage.

On the other hand, in order to eliminate inflammation, substances that remove inflammatory sources and reduce biological reactions and symptoms are called anti-inflammatory agents. Substances used for anti-inflammatory purposes to date include flufenamic acid, ibuprofen, benzydamine, and indomethacin as a nonsteroidal type, and prednisolone as a steroid type. , Dexamethasone, and the like. In addition, allantoin, azene, hydrocortisone, etc. are known to have anti-inflammatory effects, but these substances are limited in use due to safety issues on the skin, or the effect is insignificant, so that the effect of substantially reducing inflammation cannot be expected. There is this.

Glycation refers to a reaction in which simple sugars such as glucose or fructose form covalent bonds to proteins or fats that generally occur without the involvement of enzymes. The accumulation of the final glycated product (AGE: Advanced Glycation End Product) changes the protein into a hard and brittle state, and glycated collagen prevents the collagen from forming an appropriate structure in the extracellular matrix of the dermal layer, thereby enhancing the elasticity of the skin. Loss and promotes wrinkle formation (Dyer, DG et al, The Journal of Clinical Investigation., 9, p. 2463, 1993). In addition, the final saccharification product produced by saccharification is a substance that has a brownish color, and is thought to be the causative agent that gradually turns yellow as the skin aging progresses, and as the final saccharification product accumulates, the reflected light from the skin decreases. It is known that it is. (Hiroshi, O. et al, Skin Research and Technology, 15, p. 496, 2009) Aminoguanidine, Pyridoxamine, Aspirin, etc. are known as substances that inhibit glycation. The amount of the substance is limited due to a safety problem for the skin, or there is a problem that the effect cannot be expected because the effect is insignificant.

Under this background, the present inventors have conducted research on substances that are effective in improving skin elasticity, wrinkles, or skin troubles from natural resources, and the oil extracted from Mortierella Isabelina culture is applied to the skin. It was found that it shows collagen synthesis promotion, anti-inflammatory and anti-glycosylation effects without any irritating problem, and it has come to complete the cosmetic composition, health functional food composition and quasi-drug composition using this.

It is an object of the present invention to provide a cosmetic composition comprising the oil extracted from the culture medium of Mortierella Isabelina as an active ingredient.

Specifically, it is an object of the present invention to provide a cosmetic composition for improving skin elasticity or wrinkles, comprising the oil extracted from Mortierella Isabelina culture as an active ingredient.

In addition, it is an object of the present invention to provide a cosmetic composition for improving skin troubles comprising the oil extracted from the culture solution of Mortierella Isabelina as an active ingredient.

Another object of the present invention is to provide a health functional food composition comprising as an active ingredient the oil extracted from Mortierella Isabelina culture.

Another object of the present invention is to provide a quasi-drug composition comprising the oil extracted from the culture medium of Mortierella Isabelina as an active ingredient.

The present invention provides a cosmetic composition comprising, as an active ingredient, oil extracted from Mortierella Isabellina culture solution in order to solve the above problems and achieve the object of the present invention.

Specifically, as an aspect of the present invention, there is provided a cosmetic composition for improving skin elasticity or wrinkles, comprising the oil extracted from Mortierella Isabellina culture as an active ingredient.

Specifically, as another aspect of the present invention, there is provided a cosmetic composition for improving skin troubles comprising the oil extracted from the culture solution of Mortierella Isabelina as an active ingredient.

In addition, as another aspect of the present invention, it provides a health functional food composition comprising the oil extracted from the culture broth of Mortierella Isabelina as an active ingredient.

In addition, as another aspect of the present invention, it provides a quasi-drug composition comprising the oil extracted from Mortierella Isabelina culture as an active ingredient.

Hereinafter, the present invention will be specifically described.

In the present invention, there is provided a cosmetic composition comprising an oil extracted from a culture solution of Mortierella Isabelina.

Mortierella isabellina of the present invention ( Mortierella isabellina ) is a microorganism of a fungus belonging to the Mortierella family Mortierella family. Mortierella species generally correspond to species that are not pathogenic to plants, animals and humans.

In the present invention, " Mortierella oil" is an oil obtained by culturing Mortierella isabellina . More specifically, mortierella oil is an oil derived from microorganisms and is known to be used as a moisture evaporation blocker among components of cosmetics. The method of obtaining the mortierella oil is not particularly limited, and may be prepared by a method known in the art, or a commercially available material may be used.

In one embodiment of the present invention, after adding an herbal mixture, inoculating Mortierella isabellina and incubating for 240 hours, the culture solution is filtered to separate the culture solution and the cells, and extraction consisting of chloroform and methanol The concentrate (oil) was extracted with a solvent. A dewaxing process of physically separating oil and fat into a solid phase and a liquid phase by storing the concentrate at refrigeration and freezing temperatures; A degumming process of removing gum substances and impurities from the oil and fat; After undergoing decolorization and deodorization processes in which residual oil-soluble pigment substances are adsorbed and removed, it was purified, and finally Mortierella oil was obtained.

In the present invention, "culture" means to grow microorganisms under appropriately artificially controlled environmental conditions. In addition, the "culture solution" of the present invention means a material obtained as a result of culturing microorganisms.

In the present invention, the cultivation method may be performed through a method known in the art, but specifically, may be a cultivation method through air bubbles, but is not limited thereto. In an embodiment of the present invention, a herb mixture and a seed culture of Mortierella isabellina are added, and the main culture is carried out through air bubbles to prepare a Mortierella isabelina culture solution. Obtained.

The "fermentation liquid" of the present invention is a material obtained through the fermentation process of Mortierella Isabelina by adding an herbal mixture, and may be used in the same meaning as the culture medium in the present invention. The term "fermentation" above refers to decomposing organic matter using enzymes possessed by microorganisms. More specifically, in a narrow sense, it refers to the process of decomposing sugars in which microorganisms obtain energy without using oxygen, and in a broader sense, it refers to a process in which a substance useful to humans is obtained using microorganisms or fungi.

In the present invention, the "extract" refers to an extract obtained by filtering the culture solution of Mortierella Isabelina to separate the cells, extracting with an extraction solvent, a dilution or concentrate of the extract, a dried product obtained by drying the extract, and It includes the extract of all formulations that can be formed using the extract itself and the extract, such as a crude product, a purified product, or a mixture thereof.

In the extraction of the culture medium of Mortierella Isabelina of the present invention, the method of extracting the extract is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method may include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, and these may be performed alone or in combination of two or more methods.

In the present invention, the type of the extraction solvent is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol, and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Organic solvents such as chloroform; Or a mixture thereof may be used, and preferably, water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in combination of two or more.

In an embodiment of the present invention, a concentrate (oil) of Mortierella Isabelina culture was extracted using an extraction solvent consisting of chloroform and methanol.

"Oil" of the present invention is one of cosmetic compositions containing vegetable, mineral or animal oils, and may specifically mean face oil. The term oil may have the same meaning as "fermented oil" in the present invention.

In the present invention, "improving skin elasticity" is to alleviate the degree of sagging or sagging of the skin, and elastic fibers composed of elastin exist together with collagen fibers called collagen, in which elastin and collagen are sufficiently present It means that the skin elasticity is maintained.

In the present invention, "wrinkle improvement" refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating wrinkles that have already been generated.

In one experimental example of the present invention, in order to confirm the effect of promoting collagen synthesis of Mortierella oil, the collagen synthesis rate was confirmed. As a result, the experimental group to which Mortierella oil was added showed an excellent collagen synthesis rate compared to the control group (Table 1). Through this, it was confirmed that the composition containing the oil extracted from the culture solution of Mortierella Isabelina of the present invention can be used for enhancing skin elasticity or improving wrinkles.

In the present invention, "skin trouble" means a skin disease that may be caused by inflammation caused by excessive nitric oxide production in macrophages. The skin problems are all included regardless of the type of skin diseases related to inflammation, and non-limiting examples of skin diseases related to inflammation include atopic dermatitis, psoriasis, erythematous disease triggered by radiation, chemicals, burns, etc. Inflammatory hair loss such as acid burn, bullous skin disease, lichen shape type disease, itching due to allergies, seborrheic eczema, rose acne, pemphigus vulgaris, polymorphic exudative erythema, nodular erythema, balanitis, vulvitis, alopecia areata, skin T- Cellular lymphoma and the like, and specifically, skin rash, acne, pimples, rosacea (red nose), and the like, but are not limited thereto.

In addition, in the present invention, "improving skin troubles" refers to suppressing or inhibiting the generation of troubles on the skin, or alleviating already generated troubles.

In the present invention, "anti-inflammatory" refers to suppressing inflammation, and the inflammation is one of the defense responses of living tissues against certain stimuli, and refers to a complex lesion that causes three types of tissue deterioration, circulatory disorders and effusion, and tissue proliferation . More specifically, inflammation is part of innate immunity, and as in other animals, innate immunity in humans recognizes patterns on the cell surface that are specific to pathogens. Phagocytes recognize cells with such surfaces as non-magnetic and attack pathogens. If pathogens break through the body's physical barriers, an inflammatory reaction occurs. The inflammatory reaction is a non-specific defense action that creates a hostile environment against microorganisms invading the wound. In the inflammatory reaction, when a wound or an external infectious agent enters the body, white blood cells responsible for the initial stage immune response gather and express cytokines. Therefore, the amount of expression of cytokines in cells becomes an indicator of activation of the inflammatory response. The anti-inflammatory of the present invention may be to suppress inflammation caused by the skin.

In one experimental example of the present invention, in order to confirm the anti-inflammatory effect of Mortierella oil, the inhibitory effect of nitric oxide (NO) production was confirmed. As a result, in the case of the experimental group to which Mortierella oil was added, compared to the control group, the NO production inhibition rate and anti-inflammatory effect were shown (Table 2). Through this, it was confirmed that the composition containing the oil extracted from the culture medium of Mortierella Isabelina of the present invention can be used for improving skin problems.

In addition, in an experimental example of the present invention, in order to confirm the anti-glycosylation effect of Mortierella oil, the saccharification inhibition rate of various oil samples, including a composition containing oil extracted from Mortierella Isabelina culture, was measured. As a result, it was found that the experimental group to which Mortierella oil was added exhibited an excellent anti-glycosylation effect compared to the control group (Table 3). Through this, it was found that the composition containing the oil extracted from the culture broth of Mortierella Isabelina of the present invention serves as an auxiliary role in the activities such as wrinkle improvement and anti-inflammatory, and a synergistic effect can be expected.

The cosmetic composition of the present invention is a solution, external ointment, cream, foam, nutritional lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid cleaning agent, bathing agent, sunscreen cream, sun oil, suspension, emulsion It can be prepared in a formulation selected from the group consisting of liquid, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch, and spray, but is limited thereto. no.

In addition, the cosmetic composition of the present invention may additionally include one or more cosmetically acceptable carriers blended in general skin cosmetics, and as common ingredients, for example, oil, water, surfactant, moisturizer, lower alcohol, A thickener, a chelating agent, a colorant, a preservative, a fragrance, and the like may be appropriately blended, but the present invention is not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation.

When the formulation of the present invention is an ointment, paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures of these can be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or mixtures thereof may be used as a carrier component, and in particular, in the case of a spray, additional chloro Propellants such as fluorohydrocarbon, propane/butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil may be used, and in particular, cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol fatty ester, polyethylene glycol or fatty acid ester of sorbitan may be used. have.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable oil, glycerol, sugar, etc. may be used as a carrier component. I can.

In another aspect of the present invention, it provides a health functional food composition comprising the oil extracted from Mortierella Isabellina culture.

Specifically, it is possible to provide a health functional food composition for enhancing skin elasticity, improving wrinkles, or improving skin troubles, including oil extracted from Mortierella Isabelina culture. "Improve skin elasticity", "Improve wrinkles" and "Improve skin troubles" are as described above.

In the present invention, "health functional food" refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids and pills, using raw materials or ingredients having useful functions for the human body. Here, the term "functionality" means obtaining an effect useful for health purposes such as controlling nutrients or physiological effects on the structure and function of the human body. The health functional food of the present invention can be prepared by a method commonly used in the art, and at the time of the production, it can be prepared by adding raw materials and ingredients commonly added in the art. In addition, the formulation of the health functional food may be prepared without limitation as long as it is a formulation recognized as a health functional food. The food composition of the present invention can be prepared in various forms of formulation, and unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long time using food as a raw material, and is excellent in portability. Health functional foods can be consumed as a supplement to improve skin regeneration, exfoliation, skin elasticity, wrinkles or skin troubles.

There is no limitation on the form that the health functional food of the present invention can take, and may include all foods in a conventional sense, and may be mixed with terms known in the art such as functional foods. In addition, the health functional food of the present invention can be prepared by mixing suitable other auxiliary ingredients and known additives that may be included in the food according to the choice of a person skilled in the art. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and There are vitamin complexes and the like, and can be prepared by adding to juice, tea, jelly, juice, etc. prepared using the compound represented by Formula 1 according to the present invention as a main component. It also includes foods used as feed for animals.

As another aspect of the present invention, it provides a quasi-drug composition comprising an oil extracted from a culture solution of Mortierella Isabelina.

Specifically, it is possible to provide a quasi-drug composition for enhancing skin elasticity, improving wrinkles, or improving skin troubles, including oil extracted from Mortierella Isabelina culture. "Improve skin elasticity", "Improve wrinkles" and "Improve skin troubles" are as described above.

The quasi-drug composition of the present invention may further include a pharmaceutically acceptable carrier, excipient, or diluent as needed in addition to the above ingredients. The pharmaceutically acceptable carrier, excipient, or diluent is not limited as long as it does not impair the effects of the present invention, for example, fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, preservatives, etc. Can include.

Representative examples of the pharmaceutically acceptable carrier, excipient or diluent of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, gum acacia, alginate, calcium phosphate, calcium Carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil , Injectable esters, witepsol, macrogol, Tween 61, cacao butter, laurage, and the like.

In addition, when the composition containing the oil extracted from the culture medium of Mortierella Isabelina of the present invention is used as a quasi-drug, it may further contain one or more active ingredients exhibiting the same or similar function. For example, it may contain known skin elasticity enhancement, wrinkle improvement, moisturizing, or anti-inflammatory ingredients. When additional skin elasticity enhancement, wrinkle improvement, moisturizing, or anti-inflammatory components are included, the effect of improving skin elasticity, wrinkle improvement, moisturizing, or skin trouble improvement of the composition of the present invention may be further increased. When the above ingredients are added, skin safety, ease of formulation, and stability of active ingredients can be considered in combination with use. The quasi-drug composition is known in the art as a skin elasticity enhancement, wrinkle improvement, moisturizing or anti-inflammatory component known in the art, retinoic acid, TGF, protein derived from animal placenta, betulinic acid and chlorella extract; As anti-inflammatory components known in the art, non-steroidal flufenamic acid, ibuprofen, benzidamine, indomethacin, prednisolone, dexamethasone, allantoin, azene, hydrocortisone; And it may further include one or two or more components selected from the group consisting of derivatives thereof and various plant extracts. Additional ingredients may be included in an amount of 0.0001% to 10% by weight based on the total weight of the composition, and the content range includes requirements such as skin safety, ease of formulation of a composition containing oil extracted from Mortierella Isabellina culture solution, etc. Can be adjusted accordingly.

The quasi-drug composition of the present invention may exemplify a disinfectant cleaner, a shower foam, an ointment, a wet tissue, a coating agent, etc., but is not limited thereto, and the method of formulating a quasi-drug, a dosage, a method of use, a component, etc. It can be appropriately selected from the techniques of.

The cosmetic composition comprising the oil extracted from the culture solution of Mortierella Isabelina of the present invention is excellent in enhancing skin elasticity, improving wrinkles, and improving skin troubles. Specifically, the composition of the present invention is highly useful as a cosmetic composition for promoting skin elasticity, improving wrinkles, and improving skin troubles because it has excellent collagen synthesis promotion, anti-inflammatory and anti-glycosylation effects without problems that irritate the skin. In addition, the composition containing the oil extracted from the culture solution of Mortierella Isabelina of the present invention can be used as a health functional food composition and a quasi-drug composition.

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples and experimental examples. These Examples and Experimental Examples are for illustrative purposes only, and the scope of the present invention is not limited thereto.

Example 1 : Preparation of mortierella oil

After adding the herb mixture, inoculating Mortierella isabellina and incubating for 240 hours, the culture solution was filtered to separate the culture solution and the cells, and the concentrate (oil) was prepared with an extraction solvent consisting of chloroform and methanol. Extracted. A dewaxing process of physically separating oil and fat into a solid phase and a liquid phase by storing the concentrate at refrigeration and freezing temperatures; A degumming process of removing gum substances and impurities from the oil and fat; After undergoing decolorization and deodorization processes in which residual oil-soluble pigment substances are adsorbed and removed, it was purified, and finally Mortierella oil was obtained.

Experimental Example 1: Confirmation of the effect of promoting collagen synthesis of mortierella oil

To confirm the collagen synthesis promoting effect of Mortierella oil of Example 1, various oil samples including Mortierella oil were treated in human dermal fibroblast cells by concentration for 24 hours, and procollagen typeC-peptide (PIP) The collagen synthesis effect was evaluated using the EIA kit method, and the results of calculating the collagen synthesis rate using Equation 1 below are shown in Table 1 below. The collagen synthesis rate of each sample corresponds to a relative value assuming that the collagen synthesis rate of the untreated group is 0%, and the positive control group is TGF-β 10 ng/ml, and the untreated group is a blank sample (DMEM).

[Equation 1]

sample Collagen synthesis rate (%) TGF-β (positive control, 10 ppb) 15.064 Mortierella Oil (10ppm) 14.266 Jojoba seed oil (10ppm) 8.387 Camellia oil (10ppm) 1.249 Macadamia Seed Oil (10ppm) 0.978

As can be seen from the results of Table 1, when compared to other plant oils, Mortierella oil showed a better collagen synthesis rate at the same concentration, and thus Mortierella oil exhibited an excellent collagen synthesis effect to improve skin elasticity or A much better effect could be expected in improving wrinkles.

Experimental Example 2: Confirmation of the anti-inflammatory effect of mortierella oil

In order to confirm the anti-inflammatory effect of Mortierella oil of Example 1, the inhibitory effect of nitric oxide (NO) production was confirmed. The inhibition of nitric oxide (NO) production was evaluated through the inhibition rate of production of nitric oxide (NO) released from RAW 264.7 macrophages to the outside of the cell. Various oil samples, including Mortierella oil, are diluted by concentration, treated with LPS, and cultured for 24 hours, and then reacted with the cell culture solution and Griess reagent to measure NO production, and NO generation using Equation 2 below. The results of calculating the inhibition rate are shown in Table 2 below. The NO production inhibition rate of each sample is a relative value assuming that the NO production inhibition rate of the negative control group is 0% and the NO production inhibition rate of the untreated group is 100%, and the positive control group is L-NMMA 20ppm, the negative control group is LPS 1ppm. , The untreated group is a blank sample (DMEM).

[Equation 2]

sample NO production inhibition rate (%) L-NMMA (positive control, 20ppm) 55.278 Mortierella oil (10ppm) 43.307 Jojoba seed oil (10ppm) 31.233 Camellia oil (10ppm) 19.722 Macadamia seed oil (10ppm) 15.278

As can be seen from the results of Table 2, when compared to L-NMMA, which is a representative anti-inflammatory drug, Mortierella oil showed excellent inhibition of NO production similar to L-NMMA even at a lower concentration. Therefore, Mortierella oil exhibited an excellent anti-inflammatory effect, and compared with other vegetable oils, a much better effect could be expected in improving skin troubles.

Experimental Example 3: Confirmation of anti-glycosylation effect of mortierella oil

In order to confirm the anti-glycosylation effect of Mortierella oil of Example 1, anti-glycosylation activity was measured using L-arginine and glucose. First, 1M L-arginine and 1M glucose were dissolved in 1M phosphate buffer solution (pH 7.4), and the sample was diluted to 100 ppm using 1M phosphate buffer solution. 1M L-arginine and 1M phosphate buffer were mixed at a ratio of 1 to 4, and then 80 μl of each was dispensed into a 96-well plate. A sample diluted to 100 ppm and 0.01M aminoguanidin to be used as a positive control were added to each of 100 μl. These samples were mixed well, and finally, glucose diluted with 1M phosphate buffer solution was added so that the final concentration of glucose was 0.1M, and then reacted at 70 for 4 hours. The degree of saccharification was measured by measuring the absorbance at 420 nm in a 96-well plate using a spectrophotometer. The glycation experimental group of Equation 3 below is an experimental group in which 1M L-arginine and 1M glucose were added to induce saccharification.In order to measure the absorbance of the sample itself, 1M L-arginine and only the sample were added without adding glucose to measure the absorbance at 420 nm. Was measured. Glycation inhibitory activity was calculated using Equation 3 below, and the results are shown in Table 3 below.

[Equation 3]

sample Inhibition rate (%) Mortierella oil (100ppm) 13.5 Jojoba seed oil (100ppm) -1.5 Camellia oil (100ppm) 6.2 Macadamia seed oil (100ppm) 10.9

As can be seen from the results of Table 3, when compared to other plant oils, Mortierella oil showed a better inhibition of saccharification. Accordingly, it was found that Mortierella oil exhibited an excellent anti-glycosylation effect, acting as an auxiliary role in anti-wrinkle and anti-inflammatory activities, and a synergistic effect can be expected.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not limiting. The scope of the present invention should be construed that all changes or modifications derived from the meaning and scope of the claims to be described later rather than the above detailed description and equivalent concepts are included in the scope of the present invention.

Claims (12)

Mortierella Isabellina ( Mortierella isabellina ) A cosmetic composition for improving skin troubles, comprising the oil extracted from the culture medium as an active ingredient.
The cosmetic composition of claim 1, wherein the extraction is extracted with one or more solvents selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms, and chloroform.
The cosmetic composition of claim 1, wherein the skin trouble is caused by the accumulation of the final glycosylation product (AGE).
The cosmetic composition of claim 1, wherein the skin trouble is caused by inflammation caused by nitrogen monoxide produced by macrophages.
The cosmetic composition of claim 1, wherein the oil improves skin problems through inhibition of saccharification.
According to claim 1, The skin trouble is skin rash; pimple; Injection (red nose); Atopic dermatitis; psoriasis; Erythematous disease; Acid burns; Bullous skin disease; Lichen shape type skin disease; Itching caused by allergies; Seborrheic eczema; Acne, including rose acne; Pemphigus vulgaris; Erythema polymorphic exudative; Nodular erythema; Balanitis; Vulvitis; Inflammatory hair loss, including alopecia areata; And cutaneous T-cell lymphoma.
The cosmetic composition of claim 6, wherein the skin trouble is selected from the group consisting of skin rash, acne, pimples, and rosacea (red nose).
The method of any one of claims 1 to 7, wherein the cosmetic composition is a solution, external ointment, cream, foam, nutrient lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, Select from the group consisting of bath products, sunscreen creams, sun oils, suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays. It characterized in that it has a formulation that is, cosmetic composition.
Mortierella Isabellina ( Mortierella isabellina ) A health functional food composition for improving skin troubles, comprising the oil extracted from the culture medium as an active ingredient.
The method of claim 9, wherein the skin trouble is skin rash; pimple; Injection (red nose); Atopic dermatitis; psoriasis; Erythematous disease; Acid burns; Bullous skin disease; Lichen shape type skin disease; Itching caused by allergies; Seborrheic eczema; Acne, including rose acne; Pemphigus vulgaris; Erythema polymorphic exudative; Nodular erythema; Balanitis; Vulvitis; Inflammatory hair loss, including alopecia areata; And that is selected from the group consisting of skin T-cell lymphoma, health functional food composition.
Mortierella Isabellina ( Mortierella isabellina ) A quasi-drug composition for improving skin problems, comprising an oil extracted from the culture medium as an active ingredient.
The method of claim 11, wherein the skin trouble is skin rash; pimple; Injection (red nose); Atopic dermatitis; psoriasis; Erythematous disease; Acid burns; Bullous skin disease; Lichen shape type skin disease; Itching caused by allergies; Seborrheic eczema; Acne, including rose acne; Pemphigus vulgaris; Erythema polymorphic exudative; Nodular erythema; Balanitis; Vulvitis; Inflammatory hair loss, including alopecia areata; And that is selected from the group consisting of skin T-cell lymphoma, quasi-drug composition.