KR20200094023A - Cosmetic composition for improving atopic dermatitis - Google Patents
Cosmetic composition for improving atopic dermatitis Download PDFInfo
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- KR20200094023A KR20200094023A KR1020190011484A KR20190011484A KR20200094023A KR 20200094023 A KR20200094023 A KR 20200094023A KR 1020190011484 A KR1020190011484 A KR 1020190011484A KR 20190011484 A KR20190011484 A KR 20190011484A KR 20200094023 A KR20200094023 A KR 20200094023A
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A—HUMAN NECESSITIES
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Abstract
Description
본 발명은 아토피 피부 개선에 효과적인 화장료 조성물에 관한 것으로, 보다 상세하게는 세포증식능과 피부 세포 재생을 촉진하는 아토피 피부 개선용 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition effective for improving atopic skin, and more particularly, to a cosmetic composition for improving atopic skin that promotes cell proliferation and skin cell regeneration.
피부는 인체의 일차 방어막으로서 온도와 습도 변화, 자외선, 공해물질 등 외부환경의 자극으로부터 체내의 기관들을 보호해 주며, 체온조절 등의 생체 항상성 유지에 중요한 역할을 한다. 이런 피부의 맨 바깥에 있는 각질은 피부의 세포가 변화되어 생긴 조직이며 각질층은 완전히 죽은 세포로 구성되어있다. 이러한 각질층은 피부를 견고하게 하면서 피부조직 가장 외부에서 우리 몸의 수분을 지켜주고 밖에서 들어오는 여러 가지 물질들을 방어하는 방어선과 같은 역할을 한다.Skin is the primary protective layer of the human body and protects organs in the body from stimulation of the external environment, such as temperature and humidity changes, ultraviolet rays, pollutants, and plays an important role in maintaining bio homeostasis such as body temperature control. The keratin on the outermost surface of the skin is a tissue formed by changes in the cells of the skin, and the stratum corneum consists of completely dead cells. This stratum corneum acts as a defensive line that protects the body's moisture from the outermost part of the skin tissue and defends various substances from the outside while strengthening the skin.
피부 세포의 생성과 각질화 과정은 피부 건강을 유지하는 중요한 사이클이다. 피부 세포의 재생 능력이 좋을 경우에는 피부 세포가 진피층에서 빨리 생성되어 빨리 각질화되게 되고 외부 자극에 의해 손상되고 노화된 각질이 빨리 새롭게 생성된 각질로 대체되어 건강한 피부를 유지할 수 있도록 도와주게 된다. The process of skin cell production and keratinization is an important cycle in maintaining skin health. When the regenerating ability of skin cells is good, the skin cells are quickly generated in the dermal layer and keratinized quickly, and damaged by external stimuli and aged keratin are quickly replaced by newly generated keratin to help maintain healthy skin.
이러한 피부 재생 능력을 높여 보다 건강하고 탄력있는 피부를 유지하기 위하여 종래 각종 동물, 식물, 미생물 등으로부터 얻은 생리활성물질들이 강화된 화장품을 사용함으로써 피부의 고유기능을 유지시키고 피부세포를 활성화시켜 피부노화를 효과적으로 억제하고 피부재생을 촉진시키기 위한 노력이 계속되고 있다.In order to increase the skin regeneration ability and maintain a healthier and more elastic skin, skin-aging is maintained by maintaining the intrinsic function of the skin and activating skin cells by using cosmetics with enhanced bioactive substances obtained from various animals, plants, and microorganisms. Efforts are being made to effectively suppress and promote skin regeneration.
한편, 환경적, 사회적, 심리적, 유전적 요인 등이 복합적으로 작용하여 피부의 면역기능의 불균형을 야기하는 아토피 피부염은 태아부터 성인까지 많은 사람들이 증산의 완화와 악화가 반복되는 만성 염증성 피부질환을 가진 환자가 꾸준히 증가하고 있다. 한의학에서 흔히 태열이라고 하며, 심한 가려움증을 동반하는 홍반, 발진, 진물 및 가피형성의 피부장애를 특징으로 한다. 아토피 피부염은 전 세계적으로 계속 증가추세에 있으며, 보통 소아나 유년시절에 발생하지만 성인기에 나타나 지속되기도 한다. 심한 가려움으로 인한 집중력 저하나 수면부족으로 학습능력이나 업무능력이 떨어지고, 어릴 때부터 반복되는 증상의 치료를 위해 환자와 가족이 짊어지는 많은 시간적, 경제적 손실은 물론, 외모에 의한 정신적 고통이 신체적 고통 이상으로 큰 질환이다.On the other hand, atopic dermatitis, which causes imbalance of the immune function of the skin due to a combination of environmental, social, psychological, and genetic factors, is a chronic inflammatory skin disease in which many people, from the fetus to the adult, repeat the relief and exacerbation of diarrhea. Patients who have had a steady increase. It is commonly referred to as taeyeol in Oriental Medicine, and is characterized by skin disorders of erythema, rash, swelling and skin formation accompanied by severe itching. Atopic dermatitis continues to increase worldwide, and usually occurs in childhood or childhood, but it also persists in adulthood. Physical and mental pain caused by physical appearance as well as a lot of time and economic loss that patients and their families suffer for the treatment of repeated symptoms from childhood, due to a decrease in concentration due to severe itching or lack of sleep It is an abnormally large disease.
아토피 피부염이 생기는 원인에 대해 한의학에서는 '몸 안에 뭉친 열이 독을 만들었기 때문'으로 보고 있다. 몸속 깊숙이 숨어 있던 열독이 피부층까지 올라와 각종 트러블을 일으키는 것이다. 따라서 열독이 풀리지 않는 한 아토피 피부염은 계속 재발할 수밖에 없다. 특히 임신 중에 맵고 짠 음식이나 과도한 카페인, 알코올, 인스턴트, 기름진 음식 등을 많이 섭취했거나, 자궁 안에 노폐물이 많이 쌓였거나, 자궁이 약한 경우 태내에 열이 쌓여 태아에게 아토피 피부염을 유발한다고 보고 있다. 열독은 피부 이상만을 일으키는 것이 아니라 몸의 면역력을 떨어뜨려 각종 합병증을 유발시키므로 단순히 증상만 치료하는 것이 아니라 아토피 피부염의 원인을 찾아 근본적인 치료를 해야 한다고 주장한다. 즉, 아토피 피부염의 원인이 되는 열독을 풀어 주고, 알레르기에 대항할 수 있는 자생력을 키우는 등 근본치료를 해야 재발을 막을 수 있다고 이야기하고 있다.The cause of atopic dermatitis is considered by Oriental medicine as'because the heat in the body made poison.' The heat poison hidden deep in the body rises to the skin layer and causes various troubles. Therefore, atopic dermatitis will continue to recur unless the heat poison is resolved. In particular, it is reported that during pregnancy, a lot of spicy and salty foods, excessive caffeine, alcohol, instant foods, fatty foods, or waste products in the uterus, or a weak uterus, heat build up in the womb, causing atopic dermatitis in the fetus. It is argued that fever poisoning not only causes skin abnormalities, but also decreases the body's immunity and causes various complications, so it is necessary to find the cause of atopic dermatitis, not just the symptoms. In other words, it is said that it is possible to prevent recurrence through basic treatment, such as releasing the heat poison that causes atopic dermatitis and increasing self-sustaining ability to fight allergies.
이렇게 다양하게 거론되고 있는 아토피 피부염의 발병 원인들 중 최근 중요하게 고려되고 있는 것이 비정상적 체내 항산화 시스템과 과도한 반응성 자유 라디칼의 발생이다. 원래 자유 라디칼은 면역 시스템의 일환으로 체내로 침투한 외인성 병원균을 소멸시키는 방어 기작으로도 작용하나 아토피성 피부염 환자의 체내 및 피부에서는 발생 민감도가 크게 증가하여 과도한 자유 라디칼이 오히려 피부 보호막 혹은 피부 세포를 손상시키는 것으로 알려져 있다.Among the various causes of atopic dermatitis, which have been discussed in various ways, recent important considerations are abnormal body antioxidant systems and generation of excessively reactive free radicals. Originally, free radicals also act as a defense mechanism to destroy exogenous pathogens that have penetrated into the body as part of the immune system, but the sensitivity of development in the body and skin of patients with atopic dermatitis increases significantly, causing excessive free radicals to protect the skin barrier or skin cells. It is known to damage.
이와 같은 면역 과민반응과 활성산소의 과다 발생은 근본적인 발병원인이 명확하지는 않지만 유전적 원인 이외에 주로 환경오염에 따른 다이옥신과 같은 환경호르몬의 인체 내 유입에 따른 내, 외인적 스트레스에 의해 면역체계의 기능악화 및 파괴에 의해서 발병하는 것으로 알려져 있다. 그리고 아토피성 피부염에 수반되는 피부장벽 손상, 이차적인 세균감염, 약물 부작용에 대한 감수성의 증가, 곰팡이류나 바이러스 등의 감염에 대한 저항력 감소 등도 나타나게 된다.The immune hypersensitivity reaction and the excessive occurrence of free radicals have no clear underlying cause, but the function of the immune system by internal and external stress caused by the influx of environmental hormones such as dioxin mainly due to environmental pollution in addition to the genetic cause. It is known to be caused by exacerbation and destruction. In addition, skin barrier damage accompanied by atopic dermatitis, secondary bacterial infection, increased susceptibility to drug side effects, and decreased resistance to infections such as fungi and viruses.
이러한 아토피성 피부염을 치료하는 방법으로 알레르기원을 제거하거나 면역체계를 개선시키는 방법이 고려되고 있는데, 현재 사용되고 있는 치료방법으로는 면역억제제를 사용하여 아토피 피부염에 의해 발생하는 각종 증상을 완화시키거나 또는 아토피 질환자의 피부건조를 방지하기 위하여 보습제를 도포하고 수반되는 염증을 치료하기 위하여 국소 부신피질호르몬과 같은 스테로이드제 등을 사용하고 있다. 그러나 이러한 스테로이드제를 장기간 사용할 경우 스테로이드성 여드름, 피부위축 및 모세혈관확장 등 다양한 피부 부작용을 유발하기 때문에 이러한 부작용을 나타내지 않으면서 항염증 효과를 나타내는 천연소재를 개발이 필요하다.As a method of treating such atopic dermatitis, a method of removing an allergen or improving an immune system is being considered, and currently used treatment methods include immunosuppressants to alleviate various symptoms caused by atopic dermatitis, or Moisturizers are applied to prevent skin dryness in patients with atopic diseases, and steroids such as topical adrenal cortical hormones are used to treat the accompanying inflammation. However, since long-term use of these steroids causes various skin side effects such as steroid acne, skin atrophy, and capillary expansion, it is necessary to develop natural materials that exhibit anti-inflammatory effects without exhibiting these side effects.
한편, 면역반응에 깊이 관여하는 미네랄 성분중 하나인 칼슘(Calcium)은 사람과 동물에게 필수적인 원소로 뼈와 이의 성장과 유지에 꼭 필요하고, 혈액의 응고, 근육의 수축에도 필수적인 성분이다. 또한, 칼슘은 외부의 자극으로부터 피부를 보호하는 측면에서도 중요한 역할을 한다. 표피의 칼슘 함량과 두께는 비례하는데 표피의 두께가 두꺼워지면 외부 자극으로부터의 손상과 세균&바이러스 등이 피부를 통해 침범하는 것을 막아주며, 수분 함량과 피지막을 조절해 피부 건조와 아토피 등의 피부 질환을 치료한다. On the other hand, calcium (Calcium), one of the mineral components deeply involved in the immune response, is an essential element for humans and animals, and is essential for the growth and maintenance of bones and teeth, and is an essential component for blood clotting and muscle contraction. In addition, calcium plays an important role in protecting the skin from external stimuli. Although the calcium content and thickness of the epidermis are proportional, the thickness of the epidermis prevents damage from external stimuli and invasion of bacteria and viruses through the skin, and regulates the moisture content and the sebaceous skin to cause skin diseases such as dry skin and atopy. Treat it.
이러한 측면에서 피부에 칼슘인자를 보충할 수 있는 안정화된 화장품 성분으로서 칼슘성분을 이용하는 방법에 대한 연구가 활발히 진행되고 있다.In this aspect, research into a method of using a calcium component as a stabilized cosmetic component capable of replenishing calcium factors to the skin has been actively conducted.
아울러, 특허문헌1에서는 농작물을 유효성분으로 함유하는 칼슘 보충용 약학적 조성물 및 기능성식품을 개시하고 있으나, 본 발명의 이온화 칼슘을 강화한 천연식물 추출물을 유효성분으로 함유하여 피부재생을 촉진하는 아토피 피부 개선용 화장료 조성물에 대해 아직까지 개시된 바가 없다.In addition,
(특허문헌1)(Patent Document 1)
KR 10-1119272 B1KR 10-1119272 B1
본 발명은 이온화 칼슘을 강화한 천연식물 추출물 및 폴리올을 유효성분으로 포함하여 독성 및 부작용이 거의 없을 뿐만 아니라, 세포 재생 효과가 우수한 아토피 피부 개선용 화장료 조성물을 제공하는 것을 목적으로 한다.An object of the present invention is to provide a cosmetic composition for improving atopic skin, which has little toxicity and side effects, and contains a natural plant extract and polyol having enhanced ionized calcium as an active ingredient, and has excellent cell regeneration effects.
또한, 본 발명은 피부에 도포 시 세포 재생주기를 순화하여 효과적으로 피부장벽기능을 회복하는 아토피 피부 개선용 화장료 조성물을 제공하는 것을 목적으로 한다.In addition, an object of the present invention is to provide a cosmetic composition for improving atopic skin, which effectively restores the skin barrier function by purifying the cell regeneration cycle when applied to the skin.
상기 과제의 해결을 위하여, 본 발명에 따른 이온화 칼슘이 강화된 천연식물 추출물 및 폴리올을 유효성분으로 포함하는 아토피 피부 개선용 화장료 조성물을 제공한다.In order to solve the above problems, there is provided a cosmetic composition for improving atopic skin, which includes the natural plant extract and polyol with enhanced ionized calcium according to the present invention as an active ingredient.
본 발명의 아토피 피부 개선용 화장료 조성물은 이온화 칼슘이 강화된 천연식물 추출물로써 오이, 멜론, 참외, 포도, 상추 및 수박 등에서 선택되는 하나 이상의 추출물을 포함하는 것일 수 있다.The cosmetic composition for improving atopic skin of the present invention may be a natural plant extract enhanced with ionized calcium and include one or more extracts selected from cucumber, melon, melon, grape, lettuce, and watermelon.
본 발명의 일 양태에 따른 아토피 피부 개선용 화장료 조성물에 있어서 상기 유효성분은 화장료 조성물 총 중량에 대하여 이온화 칼슘이 강화된 천연식물 추출물이 0.1 내지 10.0중량% 및 폴리올은 0.1 내지 15.0 중량%로 포함하는 것일 수 있다.In the cosmetic composition for improving atopic skin according to an aspect of the present invention, the active ingredient contains 0.1 to 10.0% by weight of the natural plant extract with enhanced ionized calcium and 0.1 to 15.0% by weight of the polyol with respect to the total weight of the cosmetic composition. May be
또한, 본 발명의 일 양태에 따른 아토피 피부 개선용 화장료 조성물에 있어서, 추가적으로 옻나무 추출물, 약용꽃 추출물 및 발효 추출물 등에서 하나 이상을 선택하여 더 포함할 수 있다.In addition, in the cosmetic composition for improving atopy skin according to an aspect of the present invention, it may additionally include one or more selected from lacquer extract, medicinal flower extract and fermentation extract.
본 발명의 일 양태에 따른 아토피 피부 개선용 화장료 조성물에 있어서, 옻나무 추출물은 우루시올이 제거된 추출물일 수 있다.In the cosmetic composition for improving atopy skin according to an aspect of the present invention, the lacquer extract may be an extract from which urushiol is removed.
본 발명의 일 양태에 따른 아토피 피부 개선용 화장료 조성물에 있어서, 약용꽃 추출물은 작약, 갈화, 국화, 계관화, 관동화, 괴화, 금은화, 도화, 모향화, 모화, 목근화, 밀몽화, 방풍화, 봉선화, 석류화, 선복화, 선화, 송화, 신이, 연화, 원화, 촉규화, 총화, 포황 및 홍람화로 이루어진 군에서 선택된 둘 이상의 꽃 추출물일 수 있다.In the cosmetic composition for improving atopy skin according to an aspect of the present invention, the medicinal flower extract is peony, brown flower, chrysanthemum, gywanhwa, kwanhwa, blackened, gold and silver, flower drawing, scented flower, mother flower, wood root, wheat dream, windproof, It may be two or more flower extracts selected from the group consisting of balsam, pomegranate, bokbok, narcissus, songhwa, shinyi, softening, wonhwa, sukhwahwa, gunhwa, hwanhwa, and honghwahwa.
본 발명의 일 양태에 따른 아토피 피부 개선용 화장료 조성물에 있어서, 발효 추출물은 석류, 매실, 마치현, 인삼 또는 홍삼으로 이루어진 군으로부터 선택되는 하나 이상의 추출물을 미생물을 통해 발효시킨 발효물일 수 있다.In the cosmetic composition for improving atopy skin according to an aspect of the present invention, the fermentation extract may be a fermentation product obtained by fermenting one or more extracts selected from the group consisting of pomegranate, plum, machihyeon, ginseng, or red ginseng through microorganisms.
본 발명의 일 양태에 따른 아토피 피부 개선용 화장료 조성물에 있어서, 이온화 칼슘이 강화된 천연식물 추출물은 이온화 칼슘 함량이 50%이상 강화된 천연식물의 추출물일 수 있다.In the cosmetic composition for improving atopic skin according to an aspect of the present invention, the ionized calcium-enriched natural plant extract may be an extract of natural plant having an ionized calcium content of 50% or more.
본 발명의 일 양태에 따른 아토피 피부 개선용 화장료 조성물에 있어서, 폴리올은 솔비톨, 폴리에틸렌글리콜, 1,3-부틸렌글리콜 또는 글리세린을 포함하는 군으로부터 선택되는 하나 이상일 수 있다.In the cosmetic composition for improving atopy skin according to an aspect of the present invention, the polyol may be one or more selected from the group comprising sorbitol, polyethylene glycol, 1,3-butylene glycol or glycerin.
본 발명의 일 양태에 따른 아토피 피부 개선용 화장료 조성물은 상기 이온화 칼슘이 강화된 추출물 100 중량부에 대하여 상기 옻나무 추출물은 10 내지 50 중량부, 약용꽃 추출물은 10 내지 70 중량부 및 발효 추출물은 10 내지 200 중량부의 비율로 혼합된 추출물을 더 포함하는 것일 수 있다.The cosmetic composition for improving atopic skin according to an aspect of the present invention is 10 to 50 parts by weight of the lacquer extract, 10 to 70 parts by weight of the medicinal flower extract and 10 to 10 parts by weight of the extract of medicinal flowers with respect to 100 parts by weight of the extract enriched with ionized calcium It may be to further include an extract mixed in a proportion of 200 parts by weight.
본 발명의 일 양태에 따른 아토피 피부 개선용 화장료 조성물은 제형이 용액, 현탁액, 유탁액, 페이스트, 젤, 크림, 로션, 파우더, 비누, 클린징, 오일, 파운데이션, 왁스 또는 스프레이 제형인 것일 수 있다.The cosmetic composition for improving atopic skin according to an aspect of the present invention may be a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing, oil, foundation, wax or spray formulation.
본 발명의 아토피 피부 개선용 화장료 조성물은 안전하고, 독성 및 부작용이 거의 없을 뿐만 아니라, 세포증식능이 우수하여 피부재생 및 피부손상 회복에 효과적이다.The cosmetic composition for improving atopy skin of the present invention is safe, has little toxicity and side effects, and is excellent in cell proliferation, and thus is effective in skin regeneration and skin damage recovery.
또한, 본 발명의 아토피 피부 개선용 화장료 조성물은 세포 재생주기를 순화하여 효과적으로 피부장벽기능을 회복하고 피부 수화 증가 및 염증 감소를 유도하여 아토피 피부 개선의 효과가 매우 우수하다.In addition, the cosmetic composition for improving atopic skin of the present invention is excellent in the effect of improving atopic skin by purifying the cell regeneration cycle, effectively restoring skin barrier function, and inducing increased skin hydration and reduced inflammation.
도 1은 사람의 각질세포인 Hacat세포에 본 발명의 화장료 조성물을 24시간 처리한 뒤 MTT assay를 실시하여 세포독성을 평가하여 나타낸 그래프이다.
도 2는 사람의 각질세포인 Hacat세포에 본 발명의 화장료 조성물을 투여하고 세포의 증식능을 BrdU assay로 평가하여 나타낸 그래프이다.
도 3은 사람의 각질세포인 Hacat세포에 스크래치(scratch)를 형성시킨 뒤 본 발명의 화장료 조성물을 48시간 동안 투여하고 손상부위를 회복하는 능력을 현미경을 통해 200배 배율로 나타낸 이미지이다.
도 4는 본 발명의 화장료 조성물이 사람의 각질세포인 Hacat세포에서 피부세포의 세포주기에 미치는 영향을 flowcytometry로 분석한 그래프이다.1 is a graph showing the evaluation of cytotoxicity by treating the cosmetic composition of the present invention with Hacat cells, which are human keratinocytes, for 24 hours, and then conducting MTT assay.
2 is a graph showing the administration of the cosmetic composition of the present invention to human keratinocytes, Hacat cells, and evaluating the proliferative capacity of the cells by BrdU assay.
Figure 3 is an image showing the ability to recover the damaged site at a magnification of 200 times through the microscopic administration of the cosmetic composition of the present invention for 48 hours after forming a scratch on human keratinocytes, Hacat cells.
Figure 4 is a graph analyzing the effect of the cosmetic composition of the present invention on the cell cycle of skin cells in human keratinocytes Hacat cells by flowcytometry.
본 발명에 따른 아토피 피부 개선용 화장료 조성물에 대하여 이하 상술하나, 이때 사용되는 기술 용어 및 과학 용어에 있어서 다른 정의가 없다면, 이 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 통상적으로 이해하고 있는 의미를 가지며, 하기의 설명에서 본 발명의 요지를 불필요하게 흐릴 수 있는 공지 기능 및 구성에 대한 설명은 생략한다.The cosmetic composition for improving atopic skin according to the present invention will be described in detail below, but unless otherwise defined in the technical terms and scientific terms used at this time, those skilled in the art to which the present invention pertains generally understand the meaning In the following description, descriptions of well-known functions and configurations that may unnecessarily obscure the subject matter of the present invention are omitted.
본 명세서에 기재하고 있는 용어, ‘세포 재생’은 손상된 부분의 세포를 다시 만들어내는 현상을 의미하며, ‘피부재생’은 특히 피부 세포를 다시 만들어 내는 것을 의미한다. As used herein, the term “cell regeneration” refers to the phenomenon of regenerating cells in a damaged area, and “skin regeneration” specifically refers to regenerating skin cells.
또한, 본 발명에서 사용되는 용어, ‘추출물’은 식물의 잎, 꽃, 줄기, 가지, 뿌리, 전초, 나무껍질 및 열매 등에서 선택되는 하나 이상에 추출용매를 가하여 당업계에 공지된 통상의 방법에 따라 추출된다. 즉, 통상적인 온도와 압력의 조건하에서 통상적인 용매를 사용하여, 냉침추출, 초음파추출, 환류 냉각추출, 열수추출, 실온추출, 가온추출, 초임계 추출 등의 방법으로 추출되는 것일 수 있으나 이에 한정되는 것은 아니다. 이때, 상기 추출용매는 이에 한정되는 것은 아니나, 물, 탄소수 1~7개의 무수 또는 함수 저급 알코올, 헥산, 클로로포름, 에틸에테르, 아세톤, 에틸아세테이트, 부틸아세테이트, 1,3-부틸렌 글리콜, 1,2-프로필렌 글리콜 및 1,3-프로필렌 글리콜 등으로 구성된 군으로부터 선택되는 하나 이상을 사용할 수 있으며, 바람직하게는 메탄올, 에탄올, 프로판올 및 부탄올에서 선택되는 저급 알코올과 물 등이 좋다. 또한, 상기 추출용매에 의해 추출된 추출물은 분획 또는 정제의 각 단계에서 얻어지는 모든 추출물, 분획물, 정제물, 그들의 희석액, 농축액 또는 건조물을 모두 포함하는 개념으로, 경우에 따라서, 이들을 동결건조, 진공건조, 열풍건조, 분무건조, 감압건조, 포말건조, 고주파건조, 적외선 건조 등의 방법을 사용하여 건조 가능하며, 건조된 추출물을 분쇄하는 과정을 더 가질 수 있다. 따라서, 상기 추출물은 여과 후 경우에 따라 건조 및 분쇄 과정과 같은 추가적인 과정을 가짐에 따라, 액상, 농축된 고상 및 동결 건조된 분말 형태를 갖는 것을 특징으로 한다.In addition, the term used in the present invention,'extract' is a plant, leaves, flowers, stems, branches, roots, outposts, bark and fruit, etc., by adding an extraction solvent to one or more conventional methods known in the art. It is extracted accordingly. That is, it may be extracted by methods such as cold immersion extraction, ultrasonic extraction, reflux cooling extraction, hot water extraction, room temperature extraction, warming extraction, supercritical extraction, etc. using a conventional solvent under conditions of normal temperature and pressure, but is not limited thereto. It does not work. At this time, the extraction solvent is not limited to this, water, anhydrous or hydrous lower alcohol having 1 to 7 carbon atoms, hexane, chloroform, ethyl ether, acetone, ethyl acetate, butyl acetate, 1,3-butylene glycol, 1, One or more selected from the group consisting of 2-propylene glycol and 1,3-propylene glycol and the like can be used. Preferably, lower alcohol and water selected from methanol, ethanol, propanol and butanol are preferred. In addition, the extract extracted by the extraction solvent is a concept that includes all of the extracts, fractions, purified products, their diluents, concentrates or dried products obtained in each step of fractions or tablets, in some cases lyophilized, vacuum dried , Hot air drying, spray drying, drying under reduced pressure, foam drying, high-frequency drying, infrared drying, and the like can be dried, and may further have a process of pulverizing the dried extract. Therefore, the extract is characterized by having a liquid, concentrated solid and freeze-dried powder form after filtering, if necessary, by having additional processes such as drying and grinding.
본 발명에서 사용되는 용어, ‘유효성분으로 포함하는’의 의미는 화장료 조성물로써, 본 발명에 따른 효능·효과를 나타낼 수 있는 정도의 유효량을 포함하는 것을 의미하는 것일 수 있다.The term used in the present invention, the meaning of'comprising as an active ingredient' may refer to a cosmetic composition, which includes an effective amount of a degree capable of exhibiting efficacy and effects according to the present invention.
본 발명에서 사용되는 용어, ‘이온화 칼슘’의 의미는 세포 내 흡수가 용이하도록 수용성으로 이온화되어 있는 칼슘이온(Ca2+) 성분을 의미한다.As used in the present invention, the term'ionized calcium' means a calcium ion (Ca 2+ ) component that is ionized to be water-soluble to facilitate absorption in cells.
본 명세서에서 사용되는 용어, ‘폴리올(Polyol)’은 다가(多價) 알코올, 즉 2개 이상의 수산기(-OH)를 가진 지방족 화합물을 의미할 수 있다. 수산기를 2개 가진 것을 글리콜 또는 디올(diol)이라고 하고, 수산기를 3개 가진 것을 글리세롤이라 하며, 수산기를 4개 가진 것을 펜타에리트리톨이라 한다.The term “polyol” used in the present specification may mean a polyhydric alcohol, that is, an aliphatic compound having two or more hydroxyl groups (-OH). The one having two hydroxyl groups is called glycol or diol, the one having three hydroxyl groups is called glycerol, and the one having four hydroxyl groups is called pentaerythritol.
본 발명의 목적을 달성하기 위하여, 본 발명은 이온화 칼슘을 강화한 천연식물 추출물 및 폴리올을 유효성분으로 함유하는 아토피 피부 개선용 화장료 조성물을 제공한다.In order to achieve the object of the present invention, the present invention provides a cosmetic composition for improving atopic skin, which contains an ionized calcium-enriched natural plant extract and polyol as an active ingredient.
본 발명의 이온화 칼슘이 강화된 천연식물은 KR 10-1119272 B1에 공지된 방법에 따른 농작물의 재배방법을 통해 천연 유래의 칼슘 성분 함량이 증가된 식물로서, 종래의 천연식물에 일반적으로 함유된 칼슘 함유량 대비 체내 흡수율이 높은 형태인 이온화 칼슘 성분이 50 내지 300%, 바람직하게는 50 내지 150% 강화된 천연식물일 수 있다.The ionized calcium-enriched natural plant of the present invention is a plant whose content of calcium derived from nature is increased through a method of cultivating a crop according to a method known in KR 10-1119272 B1, and calcium contained in conventional natural plants It may be a natural plant with 50 to 300%, preferably 50 to 150% reinforced ionized calcium component, which has a high absorption rate in the body relative to the content.
본 발명의 바람직한 양태에 따르면, 이온화 칼슘이 강화된 천연식물 추출물은 오이, 멜론, 참외, 포도, 상추 및 수박 등으로부터 하나 이상 선택될 수 있고, 바람직하게는 멜론 또는 참외일 수 있고, 보다 바람직하게는 멜론 추출물일 수 있다.According to a preferred embodiment of the present invention, the ionized calcium-enriched natural plant extract may be one or more selected from cucumber, melon, melon, grape, lettuce, and watermelon, and preferably may be melon or melon, more preferably May be a melon extract.
또한, 본 발명에서의 상기 이온화 칼슘이 강화된 천연식물 추출물은 다양한 방법, 예컨대, 냉침추출, 초음파추출, 환류 냉각추출, 열수추출, 실온추출, 가온추출, 초임계 추출 등의 방법으로 추출하고, 이를 여과한 후 건조 분쇄 과정을 거친 액상, 농축된 고상 또는 동결 건조된 분말 형태일 수 있다.In addition, the natural plant extract with enhanced ionized calcium in the present invention is extracted by various methods, such as cold extraction, ultrasonic extraction, reflux cooling extraction, hot water extraction, room temperature extraction, warming extraction, supercritical extraction, and the like. After filtering it, it may be in the form of a liquid, concentrated solid or freeze-dried powder that has undergone a dry grinding process.
상기 이온화 칼슘을 강화한 천연식물 추출물은, 이온화 칼슘을 영양성분으로 공급하여 피부진정 및 수렴작용이 뛰어나 피부면역력 증진에 도움을 주어 외부자극으로부터 피부손상을 예방하는 효능이 있다.The natural plant extract enhanced with ionized calcium is effective in preventing skin damage from external stimulation by supplying ionized calcium as a nutritional component, which is excellent in skin soothing and convergence and helps in enhancing skin immunity.
본 발명의 바람직한 양태에 따른 화장료 조성물에서 상기 이온화 칼슘이 강화된 천연식물 추출물의 사용량은 제한되지는 않으나, 상술된 효능·효과를 극대화하기 위한 측면에서, 화장료 조성물 총 중량을 기준으로 0.01 내지 20.0 중량%, 바람직하게는 0.1 내지 10.0 중량%를 범위의 함량으로 화장료 조성물에 포함할 수 있으며, 바고, 상술한 범위를 만족하면 세포재생이 보다 원활히 이루어 질 수 있다.In the cosmetic composition according to the preferred embodiment of the present invention, the amount of the natural plant extract having the enhanced ionized calcium is not limited, but in terms of maximizing the above-described efficacy and effects, 0.01 to 20.0 weight based on the total weight of the cosmetic composition %, preferably 0.1 to 10.0% by weight may be included in the cosmetic composition in an amount in the range, bargo, cell regeneration can be made more smoothly if the above-mentioned range is satisfied.
본 발명의 바람직한 양태에 따르면, 상기 폴리올은 솔비톨(SORBITOL), 폴리에틸렌글리콜(POLYETHYLENE GLYCOL), 폴리프로필렌글리콜(POLYPROPYLENE GLYCOL), 디프로필렌글리콜(DIPROPYLENE GLYCOL), 프로필렌글리콜(PROPYLENE GLYCOL), 1,3-부틸렌글리콜(1,3-BUTYLENE GLYCOL), 글리세린(GLYCERIN), 프로판디올(PROPANEDIOL), 에칠헥산디올(ETHYL HEXANEDIOL), 1,2-헥산디올(1,2-HEXANEDIOL), 피이지/피피지/폴리부틸렌글리콜-8/5/3글리세린(PEG/PPG/POLYBUTYLENE GLYCOL-8/5/3 GLYCERIN) 및 펜틸렌글리콜(PENTYLENE GLYCOL)을 포함하는 군으로부터 선택되고, 보다 바람직하게는 글리세린, 1,3-부틸렌글리콜, 솔비톨 및 폴리에틸렌글리콜을 포함하는 군으로부터 선택되며, 가장 바람직하게는 글리세린 또는 1,3-부틸렌글리콜일 수 있다.According to a preferred embodiment of the present invention, the polyol is sorbitol (SORBITOL), polyethylene glycol (POLYETHYLENE GLYCOL), polypropylene glycol (POLYPROPYLENE GLYCOL), dipropylene glycol (DIPROPYLENE GLYCOL), propylene glycol (PROPYLENE GLYCOL), 1,3- Butylene Glycol (1,3-BUTYLENE GLYCOL), Glycerin, Propanediol, Ethyl Hexanediol, 1,2-hexanediol (1,2-HEXANEDIOL), sebum/fiji /Polybutylene glycol-8/5/3 glycerin (PEG/PPG/POLYBUTYLENE GLYCOL-8/5/3 GLYCERIN) and pentylene glycol (PENTYLENE GLYCOL), more preferably glycerin, 1 ,3-butylene glycol, sorbitol and polyethylene glycol. Most preferably, it may be glycerin or 1,3-butylene glycol.
상기 폴리올은 본 발명의 화장료 조성물 총 중량에 대하여 0.1 내지 20.0 중량% 일 수 있고, 바람직하게는 0.1 내지 15.0 중량%일 수 있다.The polyol may be 0.1 to 20.0% by weight based on the total weight of the cosmetic composition of the present invention, preferably 0.1 to 15.0% by weight.
상기 폴리올 성분은 보습제로 작용하는 것으로서, 상기 폴리올 성분의 함량이 상기 범위 미만인 경우 보습력이 떨어지고, 상기 폴리올 성분의 함량이 상기 범위를 초과하는 경우 사용감이 떨어질 수 있다.The polyol component acts as a moisturizing agent. When the content of the polyol component is less than the above range, the moisturizing power is reduced, and when the content of the polyol component exceeds the above range, the feeling of use may be deteriorated.
본 발명의 바람직한 양태에 따르면, 상기 옻나무 추출물은 알러지 반응을 일으키는 우르시올은 제거되고, 옻의 유용성분으로 알려진 갈산(gallic acid)으로 대표되는 폴리페놀류와 퀘르세틴과 같은 플라보노이드류의 기능성 성분과 약리효능은 제거되지 않은 추출물인 것이 좋다.According to a preferred embodiment of the present invention, the lacquer extract is the uricol causing the allergic reaction is removed, functional components and pharmacological efficacy of flavonoids such as polyphenols and quercetin represented by gallic acid known as useful components of lacquer It is good that the extract is not removed.
이러한 우르시올이 제거된 옻나무 추출물은 상기 이온화 칼슘이 강화된 천연식물 추출물 100 중량부에 대하여 5 내지 60 중량부, 바람직하게는 10 내지 50 중량부의 범위로 더 포함되는 것일 수 있다. 상술한 범위를 만족하면 플라보노이드류의 기능성 성분과 약리효능으로 피부재생과 염증 감소에 도움을 줄 수 있다.The lacquer extract from which urushiol has been removed may be further included in a range of 5 to 60 parts by weight, preferably 10 to 50 parts by weight, based on 100 parts by weight of the natural plant extract enriched with ionized calcium. If the above-described range is satisfied, functional components and pharmacological effects of flavonoids may help skin regeneration and reduction of inflammation.
본 발명의 바람직한 양태에 따르면, 약용꽃 추출물은 작약, 갈화, 국화, 계관화, 관동화, 괴화, 금은화, 도화, 모향화, 모화, 목근화, 밀몽화, 방풍화, 봉선화, 석류화, 선복화, 선화, 송화, 신이, 연화, 원화, 촉규화, 총화, 포황 및 홍람화로 이루어진 군으로부터 둘 이상 선택되는 꽃 추출물로써 약용꽃 식물의 꽃잎, 줄기 및 가지를 수증기로 쪄서 식힌 수용액으로 약용꽃의 활성성분을 함유하여 피부진정 및 피부보습 효능를 나타낼 수 있다.According to a preferred embodiment of the present invention, the medicinal flower extract is peony, brown flower, chrysanthemum, gyehwahwa, kwanhwahwa, hwahwa, gold and silver, hwahwa, scented flowers, cotton flowers, wood root flowers, wheat dream flowers, windproof flowers, balsam flowers, pomegranate flowers, line drawings, line drawings It is a flower extract selected from two or more of the group consisting of, Songhua, Shinyi, Yeonhwa, Wonhwa, Sigyuhwa, Gunhwa, Sulfur, and Honghwahwa. The active ingredient of the medicinal flower is used as an aqueous solution by cooling the petals, stems and branches of the medicinal flower plant with water vapor. It can contain skin soothing and moisturizing effects.
상기 약용꽃 추출물은 상기 이온화 칼슘이 강화된 천연식물 추출물 100중량부에 대하여 5 내지 80 중량부, 바람직하게는 10 내지 70 중량부의 범위로 더 포함되는 것일 수 있으며, 상술한 범위를 만족하면 피부진정 및 피부보습으로 피부손상 개선에 도움을 줄 수 있다.The medicinal flower extract may be further included in the range of 5 to 80 parts by weight, preferably 10 to 70 parts by weight with respect to 100 parts by weight of the natural plant extract with enhanced ionized calcium, and if the above-mentioned range is satisfied, skin soothing And skin moisturizing to help improve skin damage.
본 발명의 바람직한 양태에 따르면, 상기 발효 추출물은 석류, 매실, 마치현, 인삼 및 홍삼으로 이루어진 군으로부터 하나이상 선택된 추출물을 자연적으로, 또는 장치, 구체적으로 회전감압농축기 및 동결건조기를 사용하여 건조시킨 후, 상기 추출물을 용매, 바람직하게는 정제수에 일정 농도로 희석한 후 미생물을 접종하고 발효시켜 제조되는 발효 추출물일 수 있다.According to a preferred embodiment of the present invention, the fermented extract is dried by using one or more extracts selected from the group consisting of pomegranate, plum, Machihyeon, ginseng and red ginseng naturally, or by using a device, specifically a rotary pressure concentrator and a freeze dryer. , It may be a fermentation extract prepared by diluting the extract to a solvent, preferably purified water to a certain concentration, and inoculating and fermenting microorganisms.
상기 미생물은 바실러스균으로 바실러스 서브틸리스, 바실러스 푸밀리스, 바실러스 리케니포미스, 바실러스 메가테리움, 바실러스 폴리퍼멘티커스, 바실러스 메센테리커스 및 이들의 혼합물로 이루어진 군으로부터 선택되는 균주일 수 있으며, 바람직하게는 바실러스 서브틸리스일 수 있다.The microorganism is Bacillus bacteria, which may be a strain selected from the group consisting of Bacillus subtilis, Bacillus fumilis, Bacillus licheniformis, Bacillus megaterium, Bacillus polypermanticus, Bacillus mesenteriaris, and mixtures thereof. And preferably may be Bacillus subtilis.
상기 발효 추출물은 각질 연화촉진, 수분공급, 세포 재생촉진 및 피부영양 공급하여 이온화 칼슘이 강화된 천연식물 추출물과 함께 사용됨으로써 피부재생이 보다 향상되도록 도움을 줄 수 있으므로, 상기 이온화 칼슘이 강화된 천연식물 추출물 100 중량부에 대하여 10 내지 300 중량부, 바람직하게는 10 내지 200 중량부의 범위로 더 포함되는 것일 수 있다.The fermented extract can be used with natural plant extracts with enhanced calcium ionization by promoting keratin softening, hydration, cell regeneration, and skin nutrition, thereby helping skin regeneration to be further improved. 10 to 300 parts by weight based on 100 parts by weight of the plant extract, preferably 10 to 200 parts by weight may be further included.
본 발명의 바람직한 양태에 따른 화장료 조성물은 아토피 피부 개선용일 수 있다. 본 발명에 따르면, 이온화 칼슘이 강화된 천연식물 추출물 및 폴리올이 유효성분으로 포함된 본 발명의 화장료 조성물을 사용하는 경우 보다 현저하게 향상된 피부재생으로 아토피 피부 개선 효능을 나타내며, 상술된 효능·효과를 동시에 제공한다. 즉, 본 발명에 따르면 복합적으로 피부 속 생물학적 매커니즘에 영향을 주어, 아토피 피부 개선 뿐 아니라 피부 세포 재생 및 피부 손상 회복에 효과를 나타낼 수 있다.The cosmetic composition according to the preferred embodiment of the present invention may be for improving atopy skin. According to the present invention, when using the cosmetic composition of the present invention containing the ionized calcium-enriched natural plant extract and polyol as an active ingredient, it exhibits atopic skin improvement efficacy with significantly improved skin regeneration, and the above-described efficacy and effects At the same time. That is, according to the present invention, it is possible to have a complex effect on the biological mechanism in the skin, thereby exhibiting an effect on skin cell regeneration and skin damage recovery as well as atopic skin improvement.
이때, 본 발명의 바람직한 양태에 따른 화장료 조성물은 상기 이온화 칼슘이 강화된 천연식물 추출물 대비 옻나무 추출물, 약용꽃 추출물 및 발효 추출물이 각각 1 : 0.1 내지 0.5 : 0.1 내지 0.7 : 0.1 내지 2.0 비율로 혼합된 추출물이 아토피 피부 개선용 화장료 조성물을 제형 내, 0.01 내지 30 중량%로 포함될 수 있으며, 바람직하게는 0.1 내지 20.0 중량%로 포함될 수 있다.At this time, in the cosmetic composition according to a preferred embodiment of the present invention, the extract of lacquer, medicinal flower extract and fermented extract, respectively, compared to the natural plant extract with enhanced ionized calcium is mixed at a ratio of 1: 0.1 to 0.5: 0.1 to 0.7: 0.1 to 2.0, respectively. The extract contains a cosmetic composition for improving atopic skin, It may be included at 0.01 to 30% by weight, preferably 0.1 to 20.0% by weight.
본 발명의 바람직한 양태에 따른 화장료 조성물은 용액, 외용 연고, 크림, 폼, 영양 화장수, 유연 화장수, 팩, 유연수, 유액, 메이크업 베이스, 에센스, 비누, 액체 세정료, 입욕제, 선 스크린 크림, 선 오일, 현탁액, 유탁액, 페이스트, 겔, 로션, 파우더, 비누, 계면 활성제-함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션, 패취 및 스프레이로 구성된 군으로부터 선택되는 제형으로 제조할 수 있으나, 이에 제한되는 것은 아니다.The cosmetic composition according to a preferred embodiment of the present invention is a solution, external ointment, cream, foam, nutrient lotion, soft lotion, pack, soft water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil , Suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays. It is not limited thereto.
또한, 본 발명에 따른 상기 화장료 조성물은 상술한 기능성 물질에 더하여 일반 피부 화장료에 배합되는 화장품학적으로 허용 가능한 담체를 1 종 이상 추가로 포함할 수 있으며, 통상의 성분으로 예를 들면 유분, 물, 계면활성제, 보습제, 저급 알코올, 증점제, 킬레이트제, 색소, 방부제, 향료 등을 적절히 배합할 수 있으나, 이에 한정되는 것은 아니다.In addition, the cosmetic composition according to the present invention may further include one or more cosmetically acceptable carriers formulated in general skin cosmetics in addition to the above-described functional substances, and for example, oil, water, Surfactants, moisturizing agents, lower alcohols, thickeners, chelating agents, coloring agents, preservatives, fragrances, etc. may be appropriately blended, but are not limited thereto.
이때, 본 발명의 화장료 조성물에 포함되는 상기 화장품학적으로 허용 가능한 담체는 화장료 조성물의 제형에 따라 다양하게 선택될 수 있는데, 일예로서 본 발명의 제형이 연고, 페이스트, 크림 또는 젤인 경우에는, 담체성분으로서 동물성 유, 식물성 유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크, 산화 아연 등이 이용될 수 있으나 이에 한정되는 것은 아니며, 이들은 단독으로 사용되거나 2 종 이상 혼합되어 사용될 수 있다.At this time, the cosmetically acceptable carrier included in the cosmetic composition of the present invention may be variously selected according to the formulation of the cosmetic composition. For example, when the formulation of the present invention is an ointment, paste, cream or gel, the carrier component As animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. may be used, but is not limited thereto, and these may be used alone or 2 It may be used by mixing more than one species.
또한, 본 발명의 제형이 용액 또는 유탁액인 경우에는, 담체 성분으로서 용매, 용해화제 또는 유탁화제 등이 이용될 수 있으며, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌 글리콜, 1,3-부틸글리콜 오일 등이 이용될 수 있고, 특히, 목화씨 오일, 땅콩 오일, 옥수수 배종 오일, 올리브 오일, 피마자 오일 및 참깨 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 이용될 수 있으나 이에 한정되는 것은 아니며, 이들은 단독으로 사용되거나 2 종 이상 혼합되어 사용될 수 있다.Further, when the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzo Eight, propylene glycol, 1,3-butylglycol oil and the like can be used, in particular, cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol or sorbitan Fatty acid esters may be used, but are not limited thereto, and these may be used alone or in combination of two or more.
또한, 본 발명의 제형이 현탁액인 경우에는, 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타하이드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있으나 이에 한정되는 것은 아니며, 이들은 단독으로 사용되거나 2 종 이상 혼합되어 사용될 수 있다.In addition, when the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, suspensions such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or trakant, etc. may be used, but is not limited thereto, and these may be used alone or in combination of two or more.
또한, 본 발명의 제형이 비누인 경우에는, 담체 성분으로서 지방산의 알칼리 금속 염, 지방산 헤미에스테르 염, 지방산 단백질 히드롤리제이트, 이세티오네이트, 라놀린 유도체, 지방족 알코올, 식물성 유, 글리세롤, 당 등이 이용될 수 있으나 이에 한정되는 것은 아니며, 이들은 단독으로 사용되거나 2 종 이상 혼합되어 사용될 수 있다.In addition, when the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars and the like as carrier components This may be used, but is not limited thereto, and these may be used alone or in combination of two or more.
상기와 같은 구성에 의하여 본 발명에 따른 이온화 칼슘이 강화된 천연식물 추출물과 폴리올을 포함하는 화장료 조성물은 종래의 식물 추출물에서 칼슘 성분 함량이 강화된 천연식물 추출물을 포함함으로써 장시간 상용하더라도 피부 안정성을 보장할 수 있을 뿐만 아니라, 피부 세포에 이온화 칼슘을 영양성분으로 공급함으로써 피부재생뿐 아니라 피부가 건조해지는 것을 예방하여 피부 진정 및 수렴작용에도 도움을 주어 아토피 피부 개선에 현저한 효과를 제공할 수 있는 장점이 있다. The cosmetic composition comprising the natural plant extract with enhanced ionized calcium according to the present invention and the polyol according to the above-described composition includes a natural plant extract with enhanced calcium content in the conventional plant extract, thereby ensuring skin stability even when used for a long time. In addition, it has the advantage of providing a remarkable effect on improving atopic skin by providing skin cells with ionized calcium as nutrients, preventing skin regeneration as well as drying and converging skin. have.
또한, 상술한 제형 전체에 있어서 담체 성분과 상술한 추출물의 배합비는 담체 성분 100 중량부에 대하여 추출물이 0.001 내지 50 중량부의 비율로 혼합될 수 있으며, 상기 배합비는 제형의 종류에 따라 달라질 수 있다.In addition, in the whole formulation described above, the mixing ratio of the carrier component and the above-described extract may be mixed in an amount of 0.001 to 50 parts by weight based on 100 parts by weight of the carrier component, and the mixing ratio may vary depending on the type of the formulation.
이하, 본 발명의 이해를 돕기 위하여 바람직한 실시예를 제시한다. 그러나 하기의 실시예는 본 발명을 보다 쉽게 이해하기 위하여 제공되는 것일 뿐, 하기 실시예는 단지 예시적인 것이지 본 발명의 범위를 어떤 식으로든 제한하기 위한 것은 아니다. 또한 본 발명에서 각 성분의 사용량에 있어, 다른 정의가 없다면 중량(g) 단위를 기준으로 한다.Hereinafter, preferred embodiments are provided to help understanding of the present invention. However, the following examples are provided only for easier understanding of the present invention, and the following examples are merely illustrative and are not intended to limit the scope of the present invention in any way. In addition, in the amount of each component in the present invention, unless otherwise defined, it is based on the weight (g) unit.
[실시예][Example]
1-1. 화장료 조성물1-1. Cosmetic composition
본 발명에 따른 아토피 피부 개선효과가 우수한 조성물은 전체 화장료 조성물 중량에 대하여, 하기 표 1에 기재된 비율로 가용화 공정을 통해 혼합한 다음 에탄올로 추출하여 본 발명에 따른 화장료 조성물을 제조하였다.The composition having excellent atopic skin improvement effect according to the present invention was mixed with a solubilization process at a ratio shown in Table 1 with respect to the total cosmetic composition weight, and then extracted with ethanol to prepare a cosmetic composition according to the present invention.
(작약, 신이, 연화, 금은화, 관동화, 감국, 홍람화)Medicinal flower extract
(Peony, Shinyi, Yeonhwa, Gold and Silver, Kwandonghwa, Gamguk, Hongramhwa)
[평가방법][Assessment Methods]
1-1. 시료 준비1-1. Sample preparation
본 발명의 평가시료는 에탄올 추출액 형태로 제조된 실시예1의 화장료 조성물을 평가용도에 적합하도록 제작하고자 rotary evaporator (Buch, Germany)로 50℃에서 감압농축하였다. 농축된 본 발명의 화장료 조성물을 액체질소에 담가 완전히 얼린 뒤 동결건조를 7일간 수행하여 액상상태의 평가시료를 확보하였다. 본 발명에 따른 화장료 조성물의 평가시료는 -20℃에 보관하면서 필요시 phosphate buffered saline에 희석하여 효능평가에 사용하였다. The evaluation sample of the present invention was concentrated under reduced pressure at 50°C with a rotary evaporator (Buch, Germany) to prepare the cosmetic composition of Example 1 prepared in the form of an ethanol extract to be suitable for evaluation purposes. The concentrated cosmetic composition of the present invention was immersed in liquid nitrogen and completely frozen, and then freeze-drying was performed for 7 days to secure an evaluation sample in a liquid state. The evaluation sample of the cosmetic composition according to the present invention was stored in -20°C and diluted in phosphate buffered saline if necessary, and used for efficacy evaluation.
1-2. 세포배양1-2. Cell culture
사람의 각질세포(keratinocyte)인 Hacat 세포는 한국세포주은행 (KCLB, Seoul, Korea)에서 분양받았으며, 10% fetal bovie serum (FBS)과 100 unit/㎖ penicillin/streptomycin이 함유된 Dulbecco’s modified eagle’s medium (DMEM) (Lonza, Walkersville, MD USA) 배지를 사용해 37℃, 5% CO2 incubator에서 배양하였다. Hacat cells, human keratinocytes, were purchased from the Korea Cell Line Bank (KCLB, Seoul, Korea), and Dulbecco's modified eagle's medium (DMEM) containing 10% fetal bovie serum (FBS) and 100 unit/ml penicillin/streptomycin. ) (Lonza, Walkersville, MD USA) cultured at 37 ℃, 5% CO 2 incubator using a medium.
1-3. 세포독성 평가 - MTT assay1-3. Cytotoxicity evaluation-MTT assay
본 발명의 실시예에서 제조된 화장료 조성물의 세포독성을 평가하기 위해 농도별로 MTT assay를 수행하였다. 구체적인 실험방법으로 사람의 각질세포(keratinocyte)인 Hacat 세포를 2×105 cells/㎖로 96-well에 100 ㎕ 씩 분주하고 24시간 동안 안정화한 후, 본 발명의 실시예 1을 70 ppm부터 1/2씩 희석하여 농도별로 처리하고 24시간 동안 CO2 incubator에서 배양하였다. 배양 후 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyl tetrazolium bromide (MTT, 0.5 ㎎/㎖, Sigma Aldrich, St. Louise, MO, USA) 용액을 각 well에 100 ㎕ 씩 처리하였다. 이후 4시간 동안 배양한 후 MTT 용액을 제거해주고, 100 ㎕의 dimethyl sulfoxide (DMSO)를 각 well에 넣어주고 30분 동안 37℃에서 방치한 후에 570 nm 파장에서 micro spectrophotometer (Molecular Device, Sunnyvale, CA, USA)로 흡광도를 측정하였다. 상대적 세포생존율은 시료를 처리하지 않은 대조군의 생존율을 100%로 설정하고 흡광도의 상대적인 값을 비교하여 구하였다.To evaluate the cytotoxicity of the cosmetic composition prepared in Examples of the present invention, MTT assay was performed by concentration. As a specific experimental method, human keratinocytes (keratinocyte) Hacat cells were dispensed 100 µl into 96-wells at 2×10 5 cells/ml and stabilized for 24 hours, and Example 1 of the present invention was from 70 ppm to 1 Diluted by 2, treated by concentration and incubated in CO 2 incubator for 24 hours. After incubation, a solution of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT, 0.5 mg/ml, Sigma Aldrich, St. Louise, MO, USA) was added to each well 100 µl Treatment. After incubation for 4 hours, the MTT solution was removed, 100 µl of dimethyl sulfoxide (DMSO) was added to each well and left at 37°C for 30 minutes, followed by micro spectrophotometer (Molecular Device, Sunnyvale, CA, at 570 nm wavelength) USA) was measured for absorbance. The relative cell viability was determined by setting the survival rate of the control group not treated with the sample to 100% and comparing the relative values of absorbance.
그 결과를 도 1에 나타내었으며, 도 1에서 알 수 있는 바와 같이, 본 발명의 화장료 조성물은 최고농도인 70 ppm 수준에 도달할 때까지 모든 세포의 생존율이 거의 100%에 가까웠다. 오히려 낮은 농도에서 본 발명의 화장료 조성물은 통계적인 유의성을 나타내지는 않았으나 세포의 생장을 촉진하는 것으로 나타났다. 따라서 본 발명의 화장료 조성물은 70 ppm이내의 농도에서는 각질세포에 대한 세포독성이 없음을 확인할 수 있었다. The results are shown in FIG. 1, and as can be seen in FIG. 1, the cosmetic composition of the present invention was nearly 100% viable until all cells reached the highest concentration of 70 ppm. Rather, at low concentrations, the cosmetic composition of the present invention did not show statistical significance, but was shown to promote cell growth. Therefore, it was confirmed that the cosmetic composition of the present invention has no cytotoxicity against keratinocytes at a concentration within 70 ppm.
1-4. 세포증식능 평가 - BrdU assay1-4. Cell Proliferation Evaluation-BrdU assay
BrdU assay는 특정 약물을 처리 시 세포에 끼치는 영향을 측정할 때 이용되는 cell proliferation을 분석하는 방법이며, Deoxyuridine에 Br이 붙어있는 피리미딘 염기 아날로그인 BrdU를 이용하여 새롭게 만들어지는 DNA를 측정하는 방식으로 BrdU specific antibody를 반응시켜 colorimetric을 이용하여 세포의 증식을 측정할 수 있다.The BrdU assay is a method of analyzing cell proliferation used to measure the effect on cells when treating a specific drug, and is a method of measuring newly created DNA using BrdU, a pyrimidine base analog with Br attached to Deoxyuridine. Cell proliferation can be measured using colorimetric by reacting BrdU specific antibody.
이에 본 발명의 실시예를 적용한 세포증식능을 평가하기 위해 농도별로 BrdU assay를 수행하였다. 구체적인 실험방법으로 사람의 각질세포(keratinocyte)인 Hacat 세포를 5×104 cells/㎖로 96-well에 100 ㎕ 씩 분주하고 24시간 동안 안정화한 뒤 본 발명의 화장료 조성물을 0.7, 7, 70 ppm의 농도로 세포에 처리하고 24시간 동안 CO2 incubator에서 배양하였다. 세포의 증식능은 BrdU Cell Proliferation Assay Kit (Cell Signaling Technology) 를 이용하여 제품 제조사의 매뉴얼에 따라 수행하였다. 각 well에 10X의 BrdU solution을 10 ㎕ 씩 넣어준 후, 24시간 동안 배양하였다. 세포 배양액을 제거한 후, Fixing/Denaturing Solution을 100 ㎕ 씩 넣어주고 30분 동안 상온에서 방치하였다. Fixing/Denaturing Solution을 제거한 후, detection antibody solution을 well 당 100 ㎕ 씩 넣어주고 1시간 동안 상온에서 방치하였다. 그 후, Washing Buffer로 3번 세척하고 HRP-conjugated secondary antibody solution을 각 well 당 100 ㎕ 씩 넣고 상온에서 30분 동안 방치하였다. 상등액을 제거한 뒤, 3번 세척을 하고 TMB substrate를 100 ㎕ 씩 넣어주었다. 상온에서 30분 방치한 후 stop solution을 100 ㎕ 씩 넣고 450 nm의 파장에서 micro spectrophotometer (Molecular Device, Sunnyvale, CA, USA)로 흡광도를 측정하여 세포의 증식능을 평가하였다.Thus, BrdU assay was performed by concentration to evaluate the cell proliferation capacity to which the examples of the present invention were applied. As a specific experimental method, the human keratinocytes (keratinocyte) Hacat cells were dispensed 100 µl in 96-well at 5×10 4 cells/ml, stabilized for 24 hours, and then the cosmetic composition of the present invention was 0.7, 7, 70 ppm. The cells were treated with the concentration of and incubated in a CO 2 incubator for 24 hours. Cell proliferation capacity was performed according to the product manufacturer's manual using the BrdU Cell Proliferation Assay Kit (Cell Signaling Technology). After adding 10 µl of 10X BrdU solution to each well, the cells were cultured for 24 hours. After removing the cell culture, 100 µl of Fixing/Denaturing Solution was added and left at room temperature for 30 minutes. After removing the Fixing/Denaturing Solution, 100 µl of detection antibody solution was added per well and left at room temperature for 1 hour. Then, washed 3 times with Washing Buffer, 100 µl of each HRP-conjugated secondary antibody solution was added to each well, and allowed to stand at room temperature for 30 minutes. After removing the supernatant, washing was performed 3 times and 100 μl of the TMB substrate was added. After standing at room temperature for 30 minutes, 100 μl of stop solution was added, and the absorbance was measured with a micro spectrophotometer (Molecular Device, Sunnyvale, CA, USA) at a wavelength of 450 nm to evaluate the cell proliferation capacity.
그 결과를 도 2에 나타내었으며, 도 2에서 알 수 있는 바와 같이, 아무것도 처리하지 않은 음성 대조군(Nor)은 흡광도의 값은 1.87±0.12였으나 본 발명의 화장료 조성물을 0.7, 7, 70 ppm으로 투여한 피부세포에서는 흡광도 값이 각각 2.13±0.13, 2.18±0.14, 2.32±0.18을 나타내어 본 발명의 화장료 조성물에 의하여 세포의 증식이 농도의존적으로 증가함을 확인할 수 있었다. 양성대조군으로 사용한 EGF(RH Human Oligopeptide-1) 투여군은 2.07±0.09를 나타낸 것과 비교하여 볼 때, 본 발명의 화장료 조성물이 피부세포 증식에 매우 효과적임을 확인 할 수 있었다.The results are shown in FIG. 2, and as can be seen in FIG. 2, the negative control (Nor) that did not process anything had an absorbance value of 1.87±0.12, but the cosmetic composition of the present invention was administered at 0.7, 7, 70 ppm. In one skin cell, the absorbance values were 2.13±0.13, 2.18±0.14, and 2.32±0.18, respectively, and thus it was confirmed that the proliferation of cells was concentration-dependently increased by the cosmetic composition of the present invention. Compared with the EGF (RH Human Oligopeptide-1) administration group used as a positive control group, it was confirmed that the cosmetic composition of the present invention was very effective in proliferating skin cells, as compared with those showing 2.07±0.09.
1-5. 피부재생효능 평가 - Wound healing assay1-5. Skin regeneration efficacy evaluation-Wound healing assay
본 발명의 실시예에서 제조된 화장료 조성물의 피부재생효능을 평가하기 위해 농도별로 Wound healing assay를 수행하였다. 구체적인 실험방법으로 사람의 각질세포(keratinocyte)인 Hacat 세포를 5×105 cells/㎖로 24-well에 500 ㎕ 씩 분주하고 24시간 동안 안정화하였다. FBS를 포함하지 않는 배지로 교체한 후 각 well에 200 μl tip을 이용하여 스크래치를 내고 각각 0.7, 7, 70 ppm 농도로 처리한 실시예 1과 ascorbic acid (Sigma Aldrich, USA)를 처리한 양성 대조군을 48시간 동안 CO2 incubator에서 배양하였다. 시료처리 후 0시간, 24시간, 48시간대에 현미경(Nikon, Japan)을 이용하여 세포 사진을 찍고 Image J 프로그램(ver1.46, NIH, USA)을 이용하여 0시간 대비 48시간에 세포가 증식된 면적을 계산하여 closure rate (%)를 구하였다.Wound healing assay was performed by concentration to evaluate the skin regeneration efficacy of the cosmetic composition prepared in the Examples of the present invention. In a specific experimental method, 500 μl of human keratinocytes (keratinocytes), Hacat cells, were dispensed at 5×10 5 cells/ml in 24-wells and stabilized for 24 hours. After replacing with FBS-free medium, each well was scratched with 200 μl tip and treated with Example 1 and ascorbic acid (Sigma Aldrich, USA) treated with concentrations of 0.7, 7, and 70 ppm, respectively. Was incubated in a CO 2 incubator for 48 hours. After sample treatment, cells were photographed using a microscope (Nikon, Japan) at 0, 24, and 48 hours, and cells were proliferated at 48 hours compared to 0 using an Image J program (ver1.46, NIH, USA). The area was calculated to determine the closure rate (%).
그 결과를 도 3에 나타내었으며, 도 3에서 알 수 있는 바와 같이, 대조군의 경우 48시간동안 26.3%의 closure rate를 나타내었다. 양성대조군으로 사용한 Ascorbic acid는 48시간동안 55.1%의 closure rate를 나타내어 대조군에 대비 피부재생효능 여부를 확인하여 본 시험이 적절히 수행되었음을 알 수 있었다. 이에 본 발명의 화장료 조성물을 각질세포에 48시간 동안 투여한 결과, 0.7 ppm에서는 21.8%의 closure rate로 피부재생효능이 거의 나타나지 않았으나, 70 ppm에서는 31.1% 및 7 ppm에서는 43.7%로 closure rate를 나타내어 피부재생효능이 있음을 알 수 있었다. 특히 투여 농도 면에서, 7 ppm일 때 closure rate 43.7%로 가장 강한 피부재생효능을 나타냄을 확인할 수 있었다.The results are shown in Fig. 3, and as can be seen in Fig. 3, the control group exhibited a closure rate of 26.3% for 48 hours. Ascorbic acid used as a positive control showed a closure rate of 55.1% for 48 hours, and it was confirmed that this test was properly performed by confirming whether or not the skin was regenerated against the control group. Thus, as a result of administering the cosmetic composition of the present invention to keratinocytes for 48 hours, the skin regeneration effect was hardly exhibited at a closure rate of 21.8% at 0.7 ppm, but the closure rate was 31.1% at 70 ppm and 43.7% at 7 ppm. It was found that there is a skin regeneration effect. Particularly, in terms of the administration concentration, it was confirmed that at 7 ppm, the closest rate of skin regeneration was exhibited at a closure rate of 43.7%.
1-6. 세포주기 순화에 대한 효능 평가 - Propidium iodide staining assay1-6. Efficacy evaluation for cell cycle purification-Propidium iodide staining assay
‘세포주기’란 세포가 분열과 성장을 통해 증식을 하게 되는 과정을 의미하는데 G1 → S → G2 → M기의 과정을 거치며 분열을 하게 된다. 이중 S기의 과정동안 세포내에 존재하는 DNA의 양이 2배가량 증가하게 되고 M기를 거치며 분열된 세포는 다시 정상적인 상태의 DNA를 포함하게 되는데, 이러한 과정의 반복으로 세포가 증식을 하고 피부재생으로 이어지는 세포주기의 변화를 유도할 수 있는지 여부를 propidium iodide (PI) staining assay를 통해 확인할 수 있다.'Cell cycle' refers to the process by which cells multiply through division and growth, and undergoes the process of G1 → S → G2 → M to divide. During the course of the S phase, the amount of DNA present in the cell doubles, and the cells that have been divided through the M phase again contain normal DNA, and by repeating this process, the cells proliferate and regenerate the skin. Whether it can induce subsequent cell cycle changes can be confirmed by propidium iodide (PI) staining assay.
이에 본 발명의 화장료 조성물 처리에 의한 세포주기 순화에 대한 효능을 평가하기 위해 농도별로 Propidium iodide staining assay를 수행하였다. 구체적인 실험방법으로 사람의 각질세포(keratinocyte)인 Hacat 세포를 1×105 cells/㎖로 60 mm에 3 ml 씩 분주하고 24시간 동안 안정화시켰다. 본 발명의 화장료 조성물을 0.7, 7, 70 ppm의 농도로 처리하고 24시간 동안 CO2 incubator에서 배양한 후, cell scraper를 이용하여 세포를 수거하였다. PBS(Phosphate Buffered Saline)로 세포를 수세한 뒤 차가운 75% ethanol로 세포를 풀어주고 -20℃ 냉동실에 24시간 동안 넣어두어 세포를 고정(fixation)하였다. Ethanol을 제거한 후 PBS로 세포를 2회 수세하여 ethanol을 완전히 제거한 뒤 PI (20 μg/ml)/RNase (100 μg/ml)을 1 ml을 30분 동안 처리하였다. 이후 flow cytometry (Becton Dickinson, USA)로 분석하였다.Accordingly, in order to evaluate the efficacy for purifying the cell cycle by treating the cosmetic composition of the present invention, a Propidium iodide staining assay was performed by concentration. As a specific experimental method, human keratinocytes (Hakat cells), 1×10 5 cells/ml, were dispensed in 3 ml increments at 60 mm and stabilized for 24 hours. After treating the cosmetic composition of the present invention at a concentration of 0.7, 7, 70 ppm and incubating in a CO 2 incubator for 24 hours, cells were collected using a cell scraper. After washing the cells with PBS (Phosphate Buffered Saline), the cells were released with cold 75% ethanol and placed in a freezer at -20°C for 24 hours to fix the cells. After removing thanol, cells were washed twice with PBS to completely remove ethanol, and then 1 ml of PI (20 μg/ml)/RNase (100 μg/ml) was treated for 30 minutes. It was then analyzed by flow cytometry (Becton Dickinson, USA).
그 결과를 도 4에 나타내었으며, 도 4에서 알 수 있는 바와 같이, 아무것도 처리하지 않은 음성 대조군(Nor)의 G1/G0, S, G2/M phase에서는 각각 43.5%, 28.6%, 15.7%의 분포를 나타내고, Ascorbic acid를 처리한 양성 대조군에서는 각각 42.0%, 26.2%, 7.03%의 분포를 나타낸 반면, 본 발명의 화장료 조성물을 0.7 ppm을 처리한 세포는 G1/G0, S, G2/M phase의 비율이 43.5%, 25.1% 3.87%로 나타났으며, 7 ppm를 처리한 세포는 각각 49.4%, 26.0%, 3.40%이었고, 70 ppm를 처리한 세포는 53.8%, 20.6%, 4.85%의 분포를 확인할 수 있었다. 이에 본 발명의 화장료 조성물이 대조군에 비해 G1/G0 phase의 비율은 농도의존적으로 증가하고 S phase의 비율과 G2/M의 비율은 감소하는 것으로 보아, 본 발명의 조성물은 피부세포의 세포주기의 순화에 영향을 주는 것을 확인할 수 있었다. The results are shown in FIG. 4, and as can be seen in FIG. 4, distribution of 43.5%, 28.6%, and 15.7% in the G1/G0, S, and G2/M phases of the negative control (Nor), which did not process anything, respectively. In the positive control group treated with Ascorbic acid, the distribution of 42.0%, 26.2%, and 7.03%, respectively, whereas cells treated with the cosmetic composition of the present invention at 0.7 ppm were G1/G0, S, and G2/M phases. The ratio was 43.5%, 25.1% and 3.87%, and cells treated with 7 ppm were 49.4%, 26.0%, and 3.40%, respectively, and cells treated with 70 ppm had a distribution of 53.8%, 20.6%, and 4.85%. I could confirm. Accordingly, as the cosmetic composition of the present invention, the ratio of the G1/G0 phase increases in a concentration-dependent manner, and the proportion of the S phase and the ratio of G2/M decrease, compared to the control group, the composition of the present invention purifies the cell cycle of skin cells It was confirmed that it affects.
이상으로 본 발명의 특정한 부분을 상세히 기술하였는 바, 당업계의 통상의 지식을 가진 자에게 있어서 이러한 구체적인 기술은 단지 구현예일 뿐이며, 이에 본 발명의 범위가 제한되는 것이 아닌 점은 명백하다. 따라서, 본 발명의 실질적인 범위는 첨부된 청구항과 그의 등가물에 의하여 정하여 진다고 할 것이다.The specific parts of the present invention have been described in detail above, and it is clear that for those skilled in the art, these specific techniques are merely implementations, and the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.
Claims (11)
상기 이온화 칼슘이 강화된 천연식물 추출물은 오이, 멜론, 참외, 포도, 상추 및 수박 중에서 선택되는 하나 이상의 추출물인 아토피 피부 개선용 화장료 조성물.According to claim 1,
The ionized calcium-enriched natural plant extract is a cosmetic composition for improving atopic skin, which is at least one extract selected from cucumber, melon, melon, grape, lettuce, and watermelon.
상기 화장료 조성물은 옻나무 추출물, 약용꽃 추출물 및 발효 추출물 중에서 하나 이상 선택하여 더 포함하는 아토피 피부 개선용 화장료 조성물.According to claim 1,
The cosmetic composition is a cosmetic composition for improving atopic skin further comprising at least one selected from lacquer extract, medicinal flower extract and fermentation extract.
상기 옻나무 추출물은 우루시올이 제거된 추출물인 아토피 피부 개선용 화장료 조성물.According to claim 3,
The lacquer extract is a cosmetic composition for improving atopic skin, which is an extract from which urushiol is removed.
상기 약용꽃 추출물은 작약, 갈화, 국화, 계관화, 관동화, 괴화, 금은화, 도화, 모향화, 모화, 목근화, 밀몽화, 방풍화, 봉선화, 석류화, 선복화, 선화, 송화, 신이, 연화, 원화, 촉규화, 총화, 포황 및 홍람화로 이루어진 군에서 선택된 둘 이상의 꽃 추출물인 아토피 피부 개선용 화장료 조성물.According to claim 3,
The medicinal flower extract is peony, brown flower, chrysanthemum, gyehwahwa, kwandonghwa, hwahwa, gold and silver, drawing, yanghwahwa, hwahwa, myohwahwa, wheat dream, windproof, balsam, pomegranate, bokhwahwa, daffodil, songhwa, shinyi, softening, A cosmetic composition for improving atopic skin, which is an extract of two or more flowers selected from the group consisting of Korean Won, Sukhwahwa, Gunhwa, Sulfur, and Hongramhwa.
상기 발효 추출물은 석류, 매실, 마치현, 인삼 또는 홍삼으로 이루어진 군으로부터 선택되는 하나 이상의 추출물을 미생물을 통해 발효시킨 발효물인 아토피 피부 개선용 화장료 조성물.According to claim 3,
The fermentation extract is a cosmetic composition for improving atopic skin, which is a fermented product fermented with one or more extracts selected from the group consisting of pomegranate, plum, Machihyeon, ginseng, or red ginseng.
상기 이온화 칼슘이 강화된 천연식물 추출물은 이온화 칼슘 함량이 50%이상 강화된 천연식물의 추출물인 아토피 피부 개선용 화장료 조성물.According to claim 1,
The ionized calcium-enriched natural plant extract is a cosmetic composition for improving atopic skin, which is an extract of a natural plant having an ionized calcium content of 50% or more.
상기 폴리올은 솔비톨, 폴리에틸렌글리콜, 1,3-부틸렌글리콜 또는 글리세린을 포함하는 군으로부터 선택되는 하나이상인 아토피 피부 개선용 화장료 조성물.According to claim 1,
The polyol is sorbitol, polyethylene glycol, 1,3-butylene glycol or a cosmetic composition for improving atopy skin, which is at least one selected from the group containing glycerin.
상기 화장료 조성물의 총 중량에 대하여,
상기 이온화 칼슘이 강화된 천연식물 추출물은 0.1 내지 10.0 중량% 및 폴리올은 0.1 내지 15.0 중량%로 포함하는 것을 특징으로 하는 아토피 피부 개선용 화장료 조성물.According to claim 1,
With respect to the total weight of the cosmetic composition,
The cosmetic composition for improving atopic skin, characterized in that the ionized calcium-enriched natural plant extract contains 0.1 to 10.0% by weight and polyol at 0.1 to 15.0% by weight.
상기 이온화 칼슘이 강화된 천연식물 추출물 100 중량부 대비 옻나무 추출물은 10 내지 50 중량부, 약용꽃 추출물은 10 내지 70 중량부 및 발효추출물은 10 내지 200 중량부의 비율로 혼합된 추출물을 포함하는 것인 아토피 피부 개선용 화장료 조성물.According to claim 3,
Compared to 100 parts by weight of the natural plant extract with enhanced ionized calcium, 10 to 50 parts by weight of lacquer extract, 10 to 70 parts by weight of medicinal flower extract and 10 to 200 parts by weight of fermented extract Atopic skin improvement cosmetic composition.
상기 화장료 조성물은 용액, 현탁액, 유탁액, 페이스트, 젤, 크림, 로션, 파우더, 비누, 클린징, 오일, 파운데이션, 왁스 또는 스프레이 제형인 것을 특징으로 하는 아토피 피부 개선용 화장료 조성물.According to claim 1,
The cosmetic composition is a cosmetic composition for improving atopy skin, characterized in that the solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing, oil, foundation, wax or spray formulation.
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