KR20200071200A - Compositions for sterilization and disinfection using hydrogen peroxide - Google Patents

Abstract

The present invention relates to a disinfectant composition using hydrogen peroxide and, more specifically, to a disinfectant composition using hydrogen peroxide, which comprises: hydrogen peroxide, citric acid, diethylene triamine penta acetic acid (DTPA) and an antibacterial agent, stabilizes hydrogen peroxide, and further improves a disinfecting effect.

Description

Sterilization and disinfectant composition using hydrogen peroxide{COMPOSITIONS FOR STERILIZATION AND DISINFECTION USING HYDROGEN PEROXIDE}

The present invention relates to a disinfectant disinfectant composition using hydrogen peroxide, and more specifically, comprises hydrogen peroxide and citric acid, diethylene triamine penta acetic acid (DTPA), and an antibacterial agent, to stabilize hydrogen peroxide and It relates to a sterilization disinfectant composition using hydrogen peroxide to further improve the disinfection effect.

Recently, awareness of disinfection is increasing due to epidemics and epidemics such as SARS and H1N1 flu, and social problems have emerged, particularly in cross- infections and infections due to the spread of viruses.

In particular, the spread of disease caused by cross-infection among patients following the outbreak of Middle East Respiratory Syndrome (MERS) in 2015 has caused great social confusion and loss, and as a result, awareness of the importance of disinfection and its management has increased.

In addition, new infectious diseases with high mortality rates such as SARS, avian influenza (AI), and MERS (MERS), which are highly infectious in Korea and abroad, are occurring periodically at intervals of 3 to 4 years.

Hydrogen peroxide is a non-halogenated oxidizing agent that is a colorless transparent viscous liquid, has redox action, decomposes by light, heat, and enzymes to become water and oxygen. Hydrogen peroxide has a strong sterilization and bleaching action and is simple to use, so it is widely used not only for disinfection of ordinary wounds, but also for the manufacture of bleaching agents and oxidizing agent derivatives. Recently, its use continues in printing institutions, semiconductor manufacturing, textile and chemical electronics industries, etc. (Inhibitory effect on Hydrgen Peroxide on the Growth of Escherichia coli.J Basic Science Vol 19: 113~117, 2004.)

The sterilization and disinfection effect by hydrogen peroxide is an oxygen radical generated by the decomposition of hydrogen peroxide, which combines with cell membrane components to show sterilization and disinfection, and manufacturers or medical institutions that mainly handle food and pharmaceuticals often use hydrogen peroxide for surface sterilization. 3% concentration of hydrogen peroxide is mainly used for disinfection with hydrogen peroxide (Microbial Contamination of Contact lens cases in multi-purpose solution care systems. Journal of Korean Ophthalmic Optics Society.Vol. 5 No. 1, 2000. pp.95-99.).

Among the prior art for sterilizing and disinfecting agents using hydrogen peroxide, Korean Patent Publication No. 10-2016-0032015 is an aqueous hydrogen peroxide solution used to reduce the number of bacteria, and is characterized by containing hydrogen peroxide, phosphate and aluminum salt as essential components. Disclosed is a sterilization method characterized in that an aqueous hydrogen peroxide solution can be provided, and air and mist and/or gas of the aqueous hydrogen peroxide solution are mixed and then brought into contact with an object.

Korea Patent Publication No. 10-2012-0028413 call the ionization of the low temperature plasma gas with hydrogen peroxide ^- by heating the ozone gas rapidly and at the same time induces a (OH, O radical) as a set temperature is injected into the sealed chamber is accused poison injected Disclosed is a sterilization sterilization apparatus and method using hydrogen peroxide, ozone, and low-temperature plasma that can greatly shorten the O-radical ionization separation time, thereby maximizing the sterilization treatment efficiency of an object to be poisoned.

However, there are many technologies that mechanically stabilize and spray unstable hydrogen peroxide to show the surface disinfection effect.

Therefore, in the present invention, it is intended to disclose a sterilization disinfectant composition that can stabilize the hydrogen peroxide in an unstable state and further improve the sterilization effect using a natural antibacterial agent having an antibacterial effect.

Korean Patent Publication No. 10-2016-0032015 Korean Patent Publication No. 10-2012-0028413

The present invention has been devised to solve the problems as described above and to provide the necessary technology,

The present invention is a hydrogen peroxide, characterized in that it is composed of a disinfectant disinfectant mixture and an antimicrobial agent is a mixture of hydrogen peroxide, citric acid and diethylene triamine penta acetic acid (DTPA) in order to provide a disinfectant composition using hydrogen peroxide. It is an object to provide a disinfectant composition using a disinfectant.

In addition, as a sterilizing and disinfecting agent composition composed of a mixture of a hydrogen peroxide, citric acid and diethylene triamine pentaacetic acid and a disinfectant disinfectant composition and an antibacterial agent, stabilizes hydrogen peroxide and further improves disinfecting effect. There are other purposes to provide.

As one embodiment of the present invention for achieving the above object,

One embodiment of the present invention is a disinfectant composition using hydrogen peroxide, characterized in that it is composed of a disinfectant disinfectant mixture and an antibacterial agent, which is a mixture of hydrogen peroxide, citric acid and diethylene triamine penta acetic acid (DTPA). to provide.

In the present invention, the disinfectant disinfectant mixture may be characterized in that 5 to 10 parts by weight of citric acid and 0.5 to 1.0 parts by weight of diethylene triamine pentaacetic acid are mixed with respect to 1000 parts by weight of hydrogen peroxide.

In addition, in the present invention, the antimicrobial agent, cypress distillate (Phytoncide), tea tree oil (Tee tree leaf Oil) and eucalyptus oil (Eucalyptus Oil) may be any one of the features.

In addition, in the present invention, the sterilizing and disinfecting agent composition may be characterized in that the composition is mixed in a proportion of 1 part by weight of the antibacterial agent with respect to 5 to 1000 parts by weight of the sterilizing and disinfecting mixture.

In addition, in the present invention, the sterilizing agent composition, sterilizing any one or more bacteria selected from Geobacillus stearothermophilus, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus Or it may be characterized by disinfection.

The sterilizing and disinfecting agent composition using hydrogen peroxide according to an embodiment of the present invention has the advantage of stabilizing hydrogen peroxide in an unstable state and further enhancing the disinfecting effect.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art to which the present invention pertains may easily practice. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art. Therefore, the embodiments of the present invention can be modified in various other forms, and the scope of the present invention is not limited to the embodiments described below.

Throughout the specification of the present invention, when a part “includes” a certain component, it means that the component may further include other components, not to exclude other components, unless otherwise specified.

The terms “about”, “substantially”, and the like used throughout the specification of the present invention are used in the sense or close to the value when manufacturing and material tolerances unique to the stated meaning are presented, and the present invention To help understand, accurate or absolute figures are used to prevent unscrupulous use of unintentional infringers by the disclosure.

The present invention provides a disinfectant composition using hydrogen peroxide.

According to an embodiment of the present invention, the sterilization disinfectant composition using hydrogen peroxide is composed of a disinfectant disinfectant mixture and an antimicrobial agent, which is a mixture of hydrogen peroxide, citric acid and diethylene triamine penta acetic acid (DTPA) It can be characterized by.

The disinfectant disinfectant mixture is characterized in that 5 to 10 parts by weight of citric acid and 0.5 to 1.0 parts by weight of DTPA are mixed with respect to 1000 parts by weight of hydrogen peroxide.

By mixing citric acid and DTPA in unstable hydrogen peroxide, as the stability of hydrogen peroxide is improved through hydrogen bonding between hydrogen peroxide and organic acid, disinfecting power is enhanced, and a safe and strong disinfecting agent can be prepared. At this time, the citric acid and DTPA are preferably used in a mixture of 5 to 10 parts by weight of citric acid and 0.5 to 1.0 parts by weight of DTPA with respect to 1000 parts by weight of hydrogen peroxide, because it is a concentration capable of maintaining hydrogen peroxide in the most stable state.

In addition, the antimicrobial agent is characterized in that any one of Cypress distillate (Phytpncide), tea tree oil (Tee tree leaf Oil) and eucalyptus oil (Eucalyptus Oil).

Cypress distillation, tea tree oil, and eucalyptus oil can be added to the antiseptic to improve the sterilizing power, as well as to counteract the sour odor peculiar to hydrogen peroxide.

In addition, the sterilizing and disinfecting agent composition is characterized in that the composition is mixed in a proportion of 1 part by weight of the antibacterial agent with respect to 5 to 1000 parts by weight of the sterilizing and disinfecting agent mixture.

When a sterilizing agent is prepared by mixing less than 1 part by weight of an antimicrobial agent with respect to 5 to 1000 parts by weight of a disinfectant disinfectant mixture, the difference in disinfecting effect may be little or no when disinfecting using only the disinfectant mixture, and the smell of hydrogen peroxide. If excessive or disinfectant is used, it may cause discomfort. When the sterilizing agent is prepared by mixing more than 1 part by weight of the antibacterial agent with respect to 5 to 1000 parts by weight of the sterilizing agent mixture, the disinfecting effect is reduced or the antibacterial agent is relatively reduced compared to hydrogen peroxide and stabilizer than when sterilizing using only the sterilizing agent mixture. As the cost of the product is high, it can be a factor of cost increase in the manufacture of disinfectants. At this time, the antibacterial agent may be used in the form of a liquid or powder. In addition, various water-soluble antibacterial agents may be mixed in addition to the above-mentioned antibacterial agents.

It is needless to say that the sterilizing and disinfecting composition using hydrogen peroxide of the present invention can be sterilized and sterilized by bacteria, viruses, etc., by applying to all objects in which the existing hydrogen peroxide disinfecting agent was used. Particularly, the sterilizing and disinfecting agent using hydrogen peroxide of the present invention is Geobacillus stearothermophilus. It can be used to sterilize and disinfect pathogenic bacteria such as Staphylococcus aureus.

Hereinafter, the present invention will be described in detail according to specific embodiments. However, the following examples are only intended to illustrate the present invention, and the contents of the present invention are not limited to the following examples.

Disinfectant disinfection

1. Preparation of disinfectant disinfectant mixture

A disinfectant disinfectant mixture is prepared by mixing 6.3 parts by weight of citric acid and 0.56 parts by weight of DTPA with respect to 1000 parts by weight of hydrogen peroxide at a concentration of 3%.

2. Preparation of sterilizing and disinfecting agents

5 to 1000 parts by weight of the above disinfectant disinfectant mixture, Cypress distillate (Phytoncide 100%. Cypress Chic), Tea Tree Oil (Melaleuca Alternifolia TeeTree Leaf Oil 100%. Integria Healthcare Pty Ltd. (Australia)) and Eucalyptus One of the oil (Eucalyptus Oil 100%. LIMS) is mixed with 1 part by weight to prepare a disinfectant. At this time, the antibacterial agent may be used in the form of a liquid or powder. In addition, various water-soluble antibacterial agents may be mixed in addition to the above-mentioned antibacterial agents.

Experiment preparation

1. Experimental strain

The strains used in the experiment are GS bacteria, ECO bacteria, PAE bacteria, and SAU bacteria.

Take out the above-mentioned strains that were kept refrigerated or frozen 24 hours before the experiment and apply them to a solid medium (Blood Agar or Mueller Hinton Agar, hereinafter referred to as MHA. Asan Pharmaceutical Co., Ltd.) using a loop, respectively. ECO bacteria, PAE bacteria, and SAU bacteria were cultured at 37±2°C for 24±2 hours, and the cultured strain was collected using a loop and released in sterile distilled water, and the concentration was adjusted to 10 ⁸ CFU/ml according to Macfaland units. Fit. At this time, the Macfaland unit is measured using EDN-18 (Biosan).

2. Measurement of disinfection effect

After incubating with the prepared sterilizing disinfectant and dissolving in sterile distilled water, the strains with the concentrations are mixed 1:1 to make 1 ml of the strain mixture in the microtube, and then left at room temperature (25±2℃) for 5 minutes, 10 GS bacteria are 55±2℃, ECO bacteria, PAE bacteria and ASU bacteria by extracting 10 µl each from the strain mixture at intervals of 30 minutes, 1 hour, 2 hours, 3 hours, 4 hours and 5 hours and dispensing them in MHA medium. The reduction was observed after incubation for 24±2 hours at 37±2℃. At this time, the Control group was used to make a strain mixture by mixing sterile distilled water and the strain 1:1.

Experiment result

1. Measurement results of disinfecting effect on disinfectant disinfectant mixtures and antibacterial agents

Using GS bacteria as a representative strain, the disinfecting effect on the disinfectant disinfectant mixture and antibacterial agent was measured, and the results are shown in Tables 1 to 3.

The first disinfecting effect of GS bacteria is a bacterium that is more resistant to sterilization than ECO and SAU, and is generally used as a biological indicator (BI) that confirms sterilization through hydrogen peroxide because it is more resistant to hydrogen peroxide than other bacteria. It is because.

As shown in Tables 1 to 3, as a result of first examining the disinfecting effect using GS as a representative strain, cypress distillate, tea tree oil, and eucalyptus in a disinfectant disinfectant mixture of citric acid and DTPA in hydrogen peroxide at a concentration of 3%. It was found that the disinfecting effect of a disinfectant prepared by mixing any one of the antibacterial agents in oil was the best.

2. Measurement result of disinfecting effect on disinfectant

Based on the experimental results in Tables 1 to 3, sterilization prepared by mixing any antibacterial agent of cypress distillate, tea tree oil, and eucalyptus oil in a sterilization disinfectant mixture in which citric acid and DTPA are mixed with hydrogen peroxide at a concentration of 3%. For the disinfectant, the disinfecting effect of GS bacteria, ECO bacteria, PAE bacteria and SAU bacteria was measured, and the results are shown in Tables 4 to 7.

As shown in Tables 4 to 7, the sterilizing and disinfecting agent using hydrogen peroxide of the present invention showed excellent sterilizing and disinfecting ability against GS bacteria, ECO bacteria, PAE bacteria, and SAU bacteria, and the sterilizing disinfectant mixture composed of citric acid and DTPA in hydrogen peroxide. In comparison, it was confirmed that the sterilizing and disinfecting agent prepared by mixing any one of the cypress distillate, tea tree oil, or eucalyptus extract in the sterilizing and disinfecting agent mixture exhibits more excellent sterilizing and disinfecting power.

As described above, the present invention has been described in detail with reference to examples, but the present invention is not limited to the above embodiments, and may be modified in various ways, and having ordinary knowledge in the art within the technical spirit of the present invention. It is clear that many variations are possible by the ruler. In addition, various forms of substitution, modification, and modification will be possible by a person having ordinary skill in the art without departing from the technical spirit of the present invention described in the claims, and this also belongs to the scope of the present invention. something to do.

Claims (5)

Disinfectant disinfectant composition using hydrogen peroxide, characterized in that it consists of a disinfectant disinfectant mixture and an antimicrobial agent, which is a mixture of hydrogen peroxide, citric acid and diethylene triamine penta acetic acid (DTPA). The method according to claim 1,
The antiseptic disinfectant mixture,
A sterilizing and disinfecting composition using hydrogen peroxide, characterized in that 5 to 10 parts by weight of citric acid and 0.5 to 1.0 parts by weight of diethylene triamine pentaacetic acid are mixed with respect to 1000 parts by weight of hydrogen peroxide. The method according to claim 1,
The antibacterial agent,
Hysteride disinfectant composition using hydrogen peroxide, characterized in that it is any one of Phytoncide, Tea tree leaf Oil and Eucalyptus Oil. The method according to claim 1,
The sterilizing agent composition,
Disinfectant disinfectant composition using hydrogen peroxide, characterized in that the composition is mixed in a proportion of 1 part by weight of the antibacterial agent with respect to 5 to 1000 parts by weight of the disinfectant disinfectant mixture. The method according to claim 1,
The sterilizing agent composition,
A bactericidal disinfectant composition using a hydrogen peroxide, characterized by sterilizing or disinfecting any one or more selected from Geobacillus stearothermophilus, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. .