KR20190081762A - Apparatus for migration of cell aggregates test and method the same - Google Patents

Abstract

The present invention includes an accommodating part in which cell aggregates are accommodated and in which a patterned patterning member can be accommodated; a pressing member located in the accommodating part and comprising a metal; and a magnetic body which has attractive force with a pressing member, and the pressing member is positioned to pressurize a portion of the patterning member by attractive force. The pressing member provides a patterning experiment apparatus which pressurizes the patterning member by contact, but provides uniform pressing force with respect to the contact area through attraction force.

Description

[0001] APPARATUS FOR MIGRATION OF CELL AGGREGATE TEST AND METHOD THE SAME [0002]

The present invention relates to an apparatus and a method for testing cell migration.

Cell migration is important for many physiological processes such as organogenesis or wound healing. In a natural environment, the direction and rate of cell movement is determined by signals that can be chemical (chemokine) or physical (microenvironment). In particular, these phenomena in the human body can be given a direction of movement to cells, for example, by using chemotactic factors, electric fields or by changing the mechanical environment of the cells. Since the chemical signal is difficult to select in a specific field, for example, in a field where an implant implanted in a human body is used, a cell guiding method based on a physical signal can be employed. Because it is very important to configure the optimal conditions that best control the movement of cells in the various conditions of the physical signal, it is required to have these physical conditions. In addition, since the cells inside the living body migrate to the group rather than move individually, an experimental method is required to quantify the movement of the population group in the laboratory.

Korean Patent Laid-Open Publication No. 2014-0109629 (Apr.

In an embodiment of the present invention, the patterning member disposed between the magnetic body and the pressing member is pressed by a uniform pressing force through the attraction force, so that the cells passing through the plurality of patterns are subjected to a patterning experiment And an object of the present invention is to provide a device.

In an embodiment of the present invention, a cell group cultured in a patterning member having a plurality of patterns is arranged so that a cell group can be moved, and a distance and direction in which cells are moved at predetermined time intervals are examined, And to provide a patterning experiment device capable of confirming the mobility of cells in accordance with the present invention.

An object of the present invention is to provide a patterning experiment apparatus which images the mobility of cells on a patterning member including a plurality of different patterns at predetermined time intervals and records the images as image information.

In one embodiment of the present invention, the patterning member having a plurality of patterns is arranged so that the cultured cell group can be moved, and the distance and direction in which the cells are moved at predetermined time intervals are experimentally determined. It is an object of the present invention to provide a patterning experimental method capable of confirming cell mobility.

 An embodiment of the present invention is to provide a patterning experiment method for imaging the mobility of cells on a patterning member including a plurality of different patterns at predetermined time intervals and recording them as image information.

A receiving portion in which the cell group is accommodated; A pressing member positioned within the receiving portion and including a metal; And a magnetic body positioned so that the pressing member is in contact with a pressing member and the pressing member presses one surface of the receiving portion with the attractive force, And a second area that is an area other than the first area, and provides a uniform pressing force to the one surface through an attractive force.

Then, the mobility of the cell group can be monitored through the degree to which the cell group is moved to the second area where the cell group is movable, by removing the pressing member.

Further, the pressing member can be pressed and contacted with the one surface through a polygonal or circular cross section.

Further, the pressing member can provide a pressing force with an area of a quadrangle.

Further, the second area may be located on the patterning member, and the patterning member may include a plurality of patterns in which at least one of the convex portion and the concave portion is different from each other.

The mobility can also be monitored through the distance that the pressing member is spaced from the receiving portion and the cell group moves on the second area for a unit time along each of the plurality of patterns.

Further, the pressing member can provide the pressing force with a circular area.

Also, the second area may be the area on the receiving portion where the pressing force is provided.

Further, mobility can be recorded by monitoring the occupation rate of the cell area occupied by the cell area per unit time, with the pressing member being spaced apart from the receiving portion.

Further, the pressing member may be a polymer-containing polymer (PDMS: Polydimethylsiloxane).

In addition, the cell population can be stained.

The imaging apparatus may further include an imaging section for image-recording the movement of the cell group at predetermined time intervals.

In addition, a pattern having widths of the convex portion and the concave portion of 5 micrometers and 5 micrometers respectively, a pattern having the widths of the convex portions and the concave portions of 5 micrometers and 10 micrometers, A pattern in which the widths of the convex portions and the concave portions are 5 micrometers and 30 micrometers, respectively, and patterns in which the widths of the convex portions and the concave portions are 20 micrometers and 10 micrometers, respectively.

Further, the plurality of patterns may be formed in a direction parallel to each other.

The inside of the accommodation portion is divided into a first area and a second area through a magnetic pressing member and a magnetic body is disposed so as to press the pressing member toward the accommodating portion side, a cell group is arranged in the first area, Thereby monitoring the movement of the base cell group located in the first area to the second area.

And, the monitoring can image the cell group moving on the second area at predetermined time intervals through the image pickup unit.

Also, the second area may be included on the patterning member received in the pressing member.

Further, the second area may be located on a plurality of patterns each including a convex portion and a concave portion.

In addition, a plurality of patterns may be used in the pattern having the widths of the convex portion and the concave portion of 5 micrometers and 5 micrometers respectively, the pattern having the widths of the convex portions and the concave portions of 5 micrometers and 10 micrometers, Meter and 20 micrometer, patterns with convex and concave widths of 5 micrometers and 30 micrometers respectively, and convex and concave widths of 20 micrometers and 10 micrometers, respectively.

The plurality of patterns may be formed in a direction parallel to each other.

The monitoring can record the distance traveled per unit time along a plurality of patterns.

The second area may be an area where the pressing member presses the receiving portion.

Monitoring may be to image the area occupied by the cell group per second time per unit time by spacing the pressure member from the second area.

The predetermined time interval may be 5 minutes or more.

In an embodiment of the present invention, the patterning member disposed between the magnetic body and the pressing member is pressed by a uniform pressing force through the attraction force, so that the cells passing through the plurality of patterns are subjected to a patterning experiment Device can be provided.

In one embodiment of the present invention, the patterning member having a plurality of patterns is arranged so that the cultured cell group can be moved, and the distance and direction in which the cells are moved at predetermined time intervals are experimentally determined. It is possible to provide a patterning experiment device capable of confirming cell mobility.

One embodiment of the present invention can provide a patterning experiment apparatus for imaging the mobility of a cell on a patterning member including a plurality of different patterns at predetermined time intervals and recording the image as image information.

In one embodiment of the present invention, the patterning member having a plurality of patterns is arranged so that the cultured cell group can be moved, and the distance and direction in which the cells are moved at predetermined time intervals are experimentally determined. It is possible to provide a patterning experiment method capable of confirming cell mobility.

 One embodiment of the present invention can provide a patterning experiment method for imaging the mobility of cells on a patterning member including a plurality of different patterns at predetermined time intervals and recording the images as image information.

1 is a view showing a patterning experiment apparatus according to an embodiment of the present invention;
2 shows a patterning member according to an embodiment of the present invention
3 is a diagram illustrating an apparatus for patterning experiment further including an image sensing unit according to another embodiment of the present invention
FIG. 4 is a view showing a cell moving direction and speed through a pattern according to an embodiment of the present invention; FIG.
5 is a diagram illustrating a patterning experiment method according to an embodiment of the present invention.
6 is a diagram illustrating a patterning experiment method according to another embodiment of the present invention.
7 is a diagram showing a patterning experiment apparatus according to another embodiment of the present invention.
8 is a view showing a second area occupation state of a cell group under various conditions according to another embodiment of the present invention

Hereinafter, specific embodiments of the present invention will be described with reference to the drawings. However, this is merely an example and the present invention is not limited thereto.

In the following description, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. The following terms are defined in consideration of the functions of the present invention, and may be changed according to the intention or custom of the user, the operator, and the like. Therefore, the definition should be based on the contents throughout this specification.

The technical idea of the present invention is determined by the claims, and the following embodiments are merely a means for effectively explaining the technical idea of the present invention to a person having ordinary skill in the art to which the present invention belongs.

The embodiment of the present invention described below is an apparatus for testing the activity of a cell. The activity includes mobility for measuring the distance the cells move per unit time and the occupancy occupying the area per unit time. Therefore, as an example for explaining the apparatus of the present invention, examples in which the mobility and the occupancy rate are monitored to confirm the activity of the cell group will be described, respectively, and the mobility of the cell group, including the patterning member, Let's start with the experimental setup.

1 is a view showing an apparatus for patterning according to an embodiment of the present invention.

Referring to FIG. 1, the patterning experiment apparatus may include a receiving unit 100, a patterning member 20, a pressing member 300, a cell group 40, and a magnetic body 500. At least one patterning member 20 having a pattern A is formed in the accommodating portion 100 and the patterning member 20 can be disposed in a state of being pressed by the pressing member 300. The cell group 40 can be disposed in the receiving portion 100, which is a space on the plane opposite to the patterning member 20, with the pressing member 300 as the center.

The cells can be placed after culturing the cultured cells, that is, a sufficiently experimental amount of cells. For example, after the incubation for 5 days. The first area A1 and the area on the patterning member 20 where the cell group 40 is to be moved are referred to as a second area A2 An embodiment will be described below.

In addition, the magnetic body 500 may be disposed on the bottom surface of the accommodating portion 100. Here, the pressing member 300 not only applies pressure by its own weight but also applies uniform pressure to the patterning member 20 (gravitation) by the gravitation acting between the pressing member 300 and the magnetic body 500 by the magnetic force of the magnetic body 500 Or on the bottom surface of the accommodating portion 100. By applying a uniform pressure to the bottom surface of the patterning member 20 or the accommodating portion 100, the same conditions can be satisfied between the respective patterns when the cells move through the plurality of patterns formed on the patterning member 20. [ The patterning member 20 having the pattern is configured to monitor the mobility of the cells moving along the pattern. When a part of the accommodating portion 100 is pressed, the pressing member 300 is removed, The pressing member 300 may function to distinguish the location of the patterning member 20 from the location of the cell group 40 so that the starting point of movement of the cell is the same.

That is, the first area A1 in which the cell group 40 is located and the second area A2 in which the cell group 40 is moved or occupied can be distinguished.

On the other hand, the pattern A may be formed on the patterning member 20. The pattern A may include convex portions (11 and 12 in Fig. 2) and concave portions (21 and 22 in Fig. 2). In this embodiment, two patterning members 20 are provided, and the pattern of the pattern to be formed on each of the patterning members 20 can be formed in different directions. For example, when the movement of the cell is expected to move in the longitudinal direction of the patterning member 20, one of the two patterning members 20 forms a pattern A in a direction parallel to the longitudinal direction, And the other one may form the pattern A in the direction perpendicular to the longitudinal direction, that is, in the transverse direction.

The mobility of the cells can be confirmed by the pattern formed in the longitudinal direction, and the extent to which the cells do not move along the longitudinal direction of the pattern and is transferred to the neighboring another pattern is confirmed by the pattern formed in the lateral direction.

On the other hand, the pattern A may be formed differently from the formed position or size information of the pattern. Here, the size information of the pattern (A) may be information including the sizes of convex portions (11 and 12 in FIG. 2) and recesses (21 and 22 in FIG. 2) included in the pattern (A). Specifically, the size information includes a depth (D1 and D2 in FIG. 2), a width (G1 and G2 in FIG. 2) and a height (D1 and D2 in FIG. 2) And the like. Such size information can be described with reference to FIG. 2 below.

2 is a cross-sectional view of a patterning member 20 according to an embodiment of the present invention.

Referring to FIG. 2, according to the example of FIG. 1, each pattern A formed on each of the neighboring patterning members 20 may be formed in a different shape from each other, Convex portions 11 and 12, and concave portions 21 and 22, respectively. Furthermore, the patterns A may be formed in different shapes between the patterns A formed in one patterning member 20. This is a condition for confirming the characteristics related to the mobility of cells, and at least one or more conditions such as the depth (D) of the recess, the width (R) of the convex portion and the width (G)

For example, the width R of the convex portion of the patterning member 20 included in the present example may be 5 micrometers, and the width G of the concave portion may be 5 to 30 micrometers. The numerical value of the width R may be a result of an experiment of cell mobility under the same conditions through a plurality of patterns A formed with different numerical values.

The experiment can be performed in the direction in which the cells are expected to move, that is, in FIG. 4 (a) in which the pattern is elongated in the longitudinal direction and in FIG. 4 (b) in the perpendicular direction.

FIG. 4A is a diagram showing data of the experiment described above. In the figure, P is a cell group cultured on a plane from the left, V1 to V4 are convex portions 11 and 12 included in the pattern A, (21, 22), respectively. Here, P to V4 in Fig. 4 (a) and P to H4 in Fig. 4 (b) are exemplary diagrams in which the width R of the convex portion is set equal to 5 micrometers and the width D of the concave portion is changed.

When the cell group 40 is positioned on a plane like the P, the cells move in various directions, but they can not move continuously in a specific direction or move away from the original position. In the case of V1, the cells move vigorously in the environment where the widths D1 and D2 of the recesses are 5 micrometers, but they move along the recesses 21 and 22, . In the case of V2 and V3, it was confirmed that in the environment where the widths D1 and D2 of the concave portions were 10 micrometers and 20 micrometers, respectively, the cells moved adjacent to the convex portions 11 and 12 and the mobility was further increased. In the case of V4, the cells are moved adjacent to the convex portions 11 and 12 in the environment where the widths D1 and D2 of the concave portions are 30 micrometers, but the widths of the concave portions 21 and 22 are increased, ) Were not able to move quickly in the lumbar region due to the distance between them.

That is, when the convex widths R 1 and R 2 are fixed at 5 micrometers, the movement of the cells is most mobility and vigorous when the widths G 1 and G 2 of the V 3 are 20 μm, (G1, G2) of 30 micrometers were found to be the least mobile. In the case of P with lower mobility, or when the cells are over the convex portions 11 and 12 and the convex portions 11 and 12 lose their guiding function, The loss of control of the direction of travel). Therefore, explaining the experiment of FIG. 4 (b) with reference to the drawings, it is possible to confirm H1 which is farthest from the convex portions 11 and 12.

It is possible to determine the degree to which the movement and proliferation of the cell can be delayed or blocked from moving from the arbitrary point A to the other point B through the above experimental result, In the opposite case, the cell movement from the point B to the point A may be selectively moved. Therefore, the structure and size information of the pattern (A) in which the differential velocity of the cells appears can be obtained so as to selectively control the movement and direction of the cells through the embodiment of the present invention.

3 is a diagram showing an apparatus for patterning experiment further including an image sensing unit 600 according to another embodiment of the present invention.

Referring to FIG. 3, it can be an experimental apparatus that further includes an image sensing unit 600 from the example of FIG. The imaging unit 600 can photograph an image of the movement of the cell group 40 on the patterning member 20 in the patterning experiment apparatus illustrated above and record the image as an image. The image may be photographed at predetermined time intervals to confirm the mobility including the movement speed of the cells. For example, the image may be recorded at predetermined time intervals for 24 hours, and the predetermined time may be 5 minutes or more. For example, images recorded for 24 hours through image recording every 10 minutes may be accumulated as frames and processed as images.

On the other hand, the cells to be photographed for image recording may be subjected to dyeing treatment to ensure cyanogenicity on the image. The dyeing process can be stained with a color that can secure a cyanide property when an image is recorded on an image pickup device. For example, cells can be stained with fluorescent light.

5 is a diagram illustrating a patterning experiment method according to an embodiment of the present invention.

The patterning experiment method may include a patterning member placement step S1, a pressing member placement step S2, a cell group placement step S3, and an image writing step S4.

First, the patterning member arranging step S1 is a step of disposing one or more patterning members 20 in the accommodating portion 100. [ Of course, as described above, the patterning member 20 may be in a state in which a pattern is formed, and the pattern may be formed in one or more patterns, and may be a pattern having different size information. Here, the pattern includes the convex portions 11 and 12 and the concave portions 21 and 22, and may be a pattern having different size information.

The size information means the size of the pattern including the width G of the concave portion, the depth D of the concave portion, and the width R of the convex portion. It is preferable that at least one of them is a pattern having different size information . For example, in the pattern, the width (R) of the convex portion may be determined to be 5 micrometers, and the width (G) of the concave portion may be determined to be 5 to 30 micrometers. Of course, this is only an example, and another size information pattern may be provided.

In this embodiment, two patterning members 20 are provided, and the pattern of the pattern to be formed on each of the patterning members 20 can be formed in different directions. For example, when the movement of the cell is expected to move in the longitudinal direction of the patterning member 20, one of the two patterning members 20 forms a pattern A in a direction parallel to the longitudinal direction, And the other one may form the pattern A in the direction perpendicular to the longitudinal direction, that is, in the transverse direction.

The mobility of the cells can be confirmed by the pattern formed in the longitudinal direction, and the extent to which the cells do not move along the pattern but shifts to the neighboring pattern can be confirmed by the pattern formed in the lateral direction.

The step S2 of arranging the pressing member means placing the pressing member 300 in the receiving portion 100. [ The pressing member 300 can be positioned to overlap the end side of the patterning member 20 to press the patterning member 20. [ The pressing member 300 may be pressed by its own weight but the pressing member 300 having the magnetic substance 500 positioned on the bottom surface of the receiving portion 100 may be pressed against the magnetic member 500 by magnetic force, The patterning member 20 interposed between the pressing member 300 and the magnetic body 500 can be pressed. This pressing may be provided to apply a uniform pressing force to the contact area between the pressing member 300 and the patterning member 20. [

In addition, the cell group arrangement step (S3) may be a step of disposing the cultured cell group (40) in the accommodating portion (100). Here, the cell group 40 may be a cultured cell for a predetermined period. For example, cataractous cells may be cultured for 5 days and placed in the accommodation section 100. Here, the arrangement can be arranged in the space on the opposite side of the patterning member 20 from the pressing member 300 in the plane.

In addition, the cell group 40 may be subjected to a staining treatment. In the image recording step S4, which is a subsequent step, the cells to be recorded on the image upon recording with the image through imaging may be stained with a predetermined color to ensure cyanogenicity.

Subsequently, in the image recording step S4, the image can be picked up through the image pickup unit 600. [ The imaging unit 600 can photograph an image of the movement of the cell group 40 on the patterning member 20 in the patterning experiment apparatus illustrated above and record the image as an image. The image may be photographed at predetermined time intervals to confirm the mobility including the movement speed of the cells. For example, the image may be recorded at predetermined time intervals for 24 hours, and the predetermined time may be 5 minutes or more. For example, images recorded for 24 hours through image recording every 10 minutes may be accumulated as frames and processed as images.

An example of image processing through photographing may be an example of FIG. The width G of the concave portion included in the pattern A may be 10 micrometers and the width G2 of the concave portion included in the second pattern B may be 30 micrometers. The numerical value of this width may be a result of an experiment of cell mobility under the same condition through a plurality of patterns formed with different numerical widths.

The experiment may be conducted in a direction parallel to the direction in which the cells are expected to move, that is, in FIG. 4 (a) in which the pattern is elongated in the longitudinal direction and in FIG.

FIG. 4A is a diagram showing data of the experiment described above. In the figure, P is a cell group cultured on a plane from the left, V1 to V4 are convex portions 11 and 12 included in the pattern A, (21, 22), respectively. Here, the width R of the convex portion is set equal to 5 micrometers, which means that the width D of the concave portion is changed.

When the cell group is located on a plane like the P, the cell moves in many directions, but does not move continuously in the specific direction or move away from the original position. In the case of V1, the cells move vigorously in the environment where the widths D1 and D2 of the recesses are 5 micrometers, but they move along the recesses 21 and 22, . In the case of V2 and V3, it was confirmed that in the environment where the widths D1 and D2 of the concave portions were 10 micrometers and 20 micrometers, respectively, the cells moved adjacent to the convex portions 11 and 12 and the mobility was further increased. In the case of V4, the cells are moved adjacent to the convex portions 11 and 12 in the environment where the widths D1 and D2 of the concave portions are 30 micrometers, but the widths of the concave portions 21 and 22 are increased, ) Were not able to move quickly in the lumbar region due to the distance between them.

That is, when the convex widths R 1 and R 2 are fixed at 5 micrometers, the movement of the cells is most mobility and vigorous when the widths G 1 and G 2 of the V 3 are 20 μm, (G1, G2) of 30 micrometers were found to be the least mobile.

When applied to an application field based on the results of this experiment, it is basically possible to guide the cell in an intended direction and control the cell migration rate. Further, although the movement or proliferation of the cells is delayed or blocked from moving from an arbitrary point A to another point B through the above experimental result, it is also possible to selectively move from the B point to the A point relatively quickly. Therefore, it is possible to acquire the structure and size information of the pattern (A) in which the differential velocity of the cells appears so that the cell movement can be selectively controlled through the embodiment of the present invention. 5 is a diagram illustrating a patterning experiment method according to an embodiment of the present invention.

The patterning experiment method may include a patterning member placement step S1, a pressing member placement step S2, a cell group placement step S3, and an image writing step S4.

First, the patterning member arranging step S1 is a step of disposing one or more patterning members 20 in the accommodating portion 100. [ Of course, as described above, the patterning member 20 may be in a state in which a pattern is formed, and the pattern may be formed in one or more patterns, and may be a pattern having different size information. Here, the pattern includes the convex portions 11 and 12 and the concave portions 21 and 22, and may be a pattern having different size information.

The size information means the size of the pattern including the width G of the concave portion, the depth D of the concave portion, and the width R of the convex portion. It is preferable that at least one of them is a pattern having different size information . For example, in the pattern, the width (R) of the convex portion may be determined to be 5 micrometers, and the width (G) of the concave portion may be determined to be 5 to 30 micrometers. Of course, this is only an example, and another size information pattern may be provided.

In this embodiment, two patterning members 20 are provided, and the pattern of the pattern to be formed on each of the patterning members 20 can be formed in different directions. For example, when the movement of the cell is expected to move in the longitudinal direction of the patterning member 20, one of the two patterning members 20 forms a pattern A in a direction parallel to the longitudinal direction, And the other one may form the pattern A in the direction perpendicular to the longitudinal direction, that is, in the transverse direction.

The mobility of the cells can be confirmed by the pattern formed in the longitudinal direction, and the extent to which the cells do not move along the pattern but shifts to the neighboring pattern can be confirmed by the pattern formed in the lateral direction.

The step S2 of arranging the pressing member means placing the pressing member 300 in the receiving portion 100. [ The pressing member 300 can be positioned to overlap the end side of the patterning member 20 to press the patterning member 20. [ The pressing member 300 may be pressed by its own weight but the pressing member 300 having the magnetic substance 500 positioned on the bottom surface of the receiving portion 100 may be pressed against the magnetic member 500 by magnetic force, The patterning member 20 interposed between the pressing member 300 and the magnetic body 500 can be pressed. This pressing may be provided to apply a uniform pressing force to the contact area between the pressing member 300 and the patterning member 20. [

In addition, the cell group arrangement step (S3) may be a step of disposing the cultured cell group (40) in the accommodating portion (100). Here, the cell group 40 may be a cultured cell for a predetermined period. For example, cataractous cells may be cultured for 5 days and placed in the accommodation section 100. Here, the arrangement can be arranged in the space on the opposite side of the patterning member 20 from the pressing member 300 in the plane.

In addition, the cell group 40 may be subjected to a staining treatment. In the image recording step S4, which is a subsequent step, the cells to be recorded on the image upon recording with the image through imaging may be stained with a predetermined color to ensure cyanogenicity.

Subsequently, in the image recording step S4, the image can be picked up through the image pickup unit 600. [ The imaging unit 600 can photograph an image of the movement of the cell group 40 on the patterning member 20 in the patterning experiment apparatus illustrated above and record the image as an image. The image may be photographed at predetermined time intervals to confirm the mobility including the movement speed of the cells. For example, the image may be recorded at predetermined time intervals for 24 hours, and the predetermined time may be 5 minutes or more. For example, images recorded for 24 hours through image recording every 10 minutes may be accumulated as frames and processed as images.

An example of image processing through photographing may be an example of FIG. The width G of the concave portion included in the pattern A may be 10 micrometers and the width G2 of the concave portion included in the second pattern B may be 30 micrometers. The numerical value of this width may be a result of an experiment of cell mobility under the same condition through a plurality of patterns formed with different numerical widths.

The experiment may be conducted in a direction parallel to the direction in which the cells are expected to move, that is, in FIG. 4 (a) in which the pattern is elongated in the longitudinal direction and in FIG.

FIG. 4A is a diagram showing data of the experiment described above. In the figure, P is a cell group cultured on a plane from the left, V1 to V4 are convex portions 11 and 12 included in the pattern A, (21, 22), respectively. Here, the width R of the convex portion is set equal to 5 micrometers, which means that the width D of the concave portion is changed.

When the cell group is located on a plane like the P, the cell moves in many directions, but does not move continuously in the specific direction or move away from the original position. In the case of V1, the cells move vigorously in the environment where the widths D1 and D2 of the recesses are 5 micrometers, but they move along the recesses 21 and 22, . In the case of V2 and V3, it was confirmed that in the environment where the widths D1 and D2 of the concave portions were 10 micrometers and 20 micrometers, respectively, the cells moved adjacent to the convex portions 11 and 12 and the mobility was further increased. In the case of V4, the cells are moved adjacent to the convex portions 11 and 12 in the environment where the widths D1 and D2 of the concave portions are 30 micrometers, but the widths of the concave portions 21 and 22 are increased, ) Were not able to move quickly in the lumbar region due to the distance between them.

That is, when the convex widths R 1 and R 2 are fixed at 5 micrometers, the movement of the cells is most mobility and vigorous when the widths G 1 and G 2 of the V 3 are 20 μm, (G1, G2) of 30 micrometers were found to be the least mobile.

When applied to an application field based on the results of this experiment, it is basically possible to guide the cell in an intended direction and control the cell migration rate. Further, although the movement or proliferation of the cells is delayed or blocked from moving from an arbitrary point A to another point B through the above experimental result, it is also possible to selectively move from the B point to the A point relatively quickly. Therefore, it is possible to acquire the structure and size information of the pattern (A) in which the differential velocity of the cells appears so that the cell movement can be selectively controlled through the embodiment of the present invention.

Hereinafter, an embodiment in which the activity of the cell group 400, that is, the occupancy rate, is monitored by another method of distinguishing the first area A1 and the second area A2 will be described.

6 is a view illustrating a patterning experiment method according to another embodiment of the present invention.

Referring to FIG. 6, the pressing member 300 may be positioned within the receiving portion 100 (P1). The pressing member 300 can be divided into the pressing area of the pressing member 300 and the pressing area of the pressing member 300. In this embodiment, (A2). Thereafter, the cell group 40 can be disposed in the receiving portion 100. The cell group 40 is disposed in the receiving portion 100 in which the pressing force is not applied from the pressing member 300, that is, (P2).

Here, the urging member 300 generates an attractive force between the magnetic bodies 500 to press the second area in the accommodating portion 100. The cell group 40 is divided into the first area A1 and the second area A2 in such a state that the pressing member 300 is separated or removed from the receiving part 100 so that the cell group 40 can move with respect to the first area A1 and the second area A2, Can be moved from the area A1 to the second area A2.

The activity of the cell group 40 can be confirmed by image recording (P3) of the cell group 40 moving to the second area A2 through the image pickup unit 600. [ The above-mentioned activity means a share occupying an area, unlike the mobility in the above-described embodiment. The difference of the occupancy rate and the mobility is the activity of the directional cell, the activity of the cell moving with the predetermined pattern along the predetermined pattern is referred to as mobility, and the occupancy of the predetermined area excluding the directionality is the occupancy rate .

Of course, in this embodiment, in order to observe the results through various experimental examples, it is needless to say that in order to observe the results, the surface of the first area A1 of the receptacle 100 in which the cell group 40 is disposed is flat, Or a condition including at least one of the following conditions. An example of such an example is shown in FIG. 8 (a diagram showing the second area occupancy state of the cell group under various conditions according to another embodiment of the present invention). Based on the results, it was found that the cell group 40 had the highest occupancy rate in the second area without any treatment according to the flow of time (72 hours standard), and the cell area (40) (40) occupied the lowest. In the second area (A2) where the micropattern was formed, the drug group was not treated, but the cell group (40) occupied less. Here, the drug used in drug treatment may be, for example, a drug such as Cyto D (Cytochalasin D).

Based on these results, it can be applied to specific fields by controlling the activity of the cell group. For example, in the medical field that manages eye diseases, it is possible to control the activity of the cell group to prevent the recurrence of the disease or to prevent the disease.

7 is a view showing an apparatus for testing cell migration according to another embodiment of the present invention.

Referring to FIG. 7, the apparatus for testing cell migration can include a receiving part 100, a pressing member 300a, and a magnetic body 500. The pressing member 300a can be brought into contact with the inside of the accommodating portion 100 by a predetermined area. The contacted area may be a circular area, and the pressing member 300a may be a magnetic material. The magnetic body 500 is located at one side of the receiving portion 100 to generate a force between the pressing members 300a and apply a uniform pressing force to the contacted area.

Since the cell group 40 is disposed after the pressing force is applied, entry of the cell group into the contacted area can be blocked. Thereby, the first area A1 and the second area A2 can be distinguished.

The first area A1 is an area in which the cell group 40 is disposed and the second area A2 is an area in which the cell group 40 can not enter by the pressing member 300a. In this state, when the pressing member 300a is separated from the receiving portion 100 or removed from the receiving portion 100, the first area A1 and the second area A2 have an area where the cell groups 40 can move relative to each other And the cell group 40 located in the first area A1 can be moved to the second area A2. The second area A2 in which the cell group 40 can not enter due to the movement can be gradually occupied by the cell group 40 due to the entry of the cell group 40. [

The occupancy per unit time can be monitored to confirm the activity of the cell population 40. Further, from the basic conditions, it is possible to carry out the process under a variety of conditions by varying the conditions of the second area A2 of the receiving portion. For example, the second area A2 may be in one or more of the following conditions: chemical treatment, nano or micro-sized pattern generation, or planar. Experiments involving the above-described process under these conditions may show results as shown in FIG. 8 (FIG. 8 is a view showing the second area occupation state of the cell group under various conditions according to another embodiment of the present invention). The cell group 40 had the highest occupancy rate in the second area without any treatment according to the flow of time (based on 72 hours), and the cell group 40 occupied the largest occupation rate on the surface of the second area A2 treated with the drug Respectively. In the second area (A2) where the micropattern was formed, the drug group was not treated, but the cell group (40) occupied less. Here, the drug used in drug treatment may be, for example, a drug such as Cyto D (Cytochalasin D).

The present invention can be applied to various fields through the above-described patterning apparatus and method. For example, it can be employed in the field of processing a pattern through a laser on the surface of an intraocular lens. When the optic part capable of passing light from the intraocular lens and having a circular shape from one side is inserted into the human body, the light is incident through the optic part, so that the object can be designed not to be positioned on the optic part. Here, the foreign body may be an ocular epithelial cell that has not been completely removed after cataract surgery. I can remove cloudy lenses from cataract patients and implant intraocular lenses. After implantation, a small amount of ocular epithelial cells that remain in the human body may cause blurring of the intraocular lens. In order to prevent this, implantation of a patterned intraocular lens to delay or block the movement or proliferation of cells in the intraocular lens . Therefore, the information of the pattern adopted in the pattern can be produced by applying the optimal pattern information according to the embodiment of the present invention, that is, the pattern information capable of selectively determining the mobility of the cells.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, . Therefore, the scope of the present invention should not be limited to the above-described embodiments, but should be determined by equivalents to the appended claims, as well as the appended claims.

11, 12:
20: patterning member
21, 22:
100:
300: pressing member
40: Cells
500: magnetic substance
A: pattern
D: Depth
G: width of lumbar
R: Breadth width
S1: patterning member placement step
S2: Pressurizing member placement step
S3: Cell placement step
S4: Image recording step

Claims (24)

A receiving portion in which the cell group is accommodated;
A pressing member located in the receiving portion and containing a metal; And
And a magnetic body positioned so that a pressing force is applied to the pressing member and the pressing member presses one surface of the receiving portion by the attractive force,
The pressing member
Wherein the inside area of the accommodating portion is divided into a first area where the cell group is located and a second area other than the first area, and a uniform pressing force is applied to the one surface through the attraction force.
The method according to claim 1,
And the mobility of the cell group can be monitored through the extent that the cell group is moved to the second area where the cell group is movable, by removing the pressing member.
The method according to claim 1,
Wherein the pressing member contacts and presses the one face through a polygonal or circular cross section.
The method of claim 3,
Wherein the pressing member provides the pressing force with a square area.
The method of claim 4,
Wherein the second area is located on the patterning member and the patterning member comprises a plurality of patterns wherein at least one of the convex and concave portions are different from each other.
The method of claim 5,
The mobility,
Wherein the pressure member is spaced apart from the receiving portion, and the cell group is monitored through a distance that the cell group moves on the second area for a unit time along each of the plurality of patterns.
The method of claim 3,
Wherein the pressing member provides the pressing force with a circular area.
The method of claim 7,
And the second area is the area on the receiving part where the pressing force is provided.
The method of claim 8,
The mobility,
Wherein the pressing member is spaced apart from the accommodating portion and the cell group is monitored through a occupation rate per unit time occupied by the second area.
The method according to claim 1,
Wherein the pressing member is PDMS (Polydimethylsiloxane) containing the metal.
The method according to claim 1,
The cell group was stained.
The method according to claim 1,
Further comprising an imaging section for image-recording the movement of the cell group at predetermined time intervals.
The method of claim 5,
A pattern in which the widths of the convex portion and the concave portion are 5 micrometers and 5 micrometers respectively, the widths of the convex portion and the concave portion are 5 micrometers and 10 micrometers, respectively, And 20 micrometers, a pattern in which the widths of the convex and concave portions are 5 micrometers and 30 micrometers, respectively, and the widths of the convex and concave portions are 20 micrometers and 10 micrometers, respectively , Cell migration experiment device.
14. The method of claim 13,
Wherein the plurality of patterns are formed in a direction parallel to each other.
The inside of the accommodation portion is divided into a first area and a second area through a magnetic pressing member,
A magnetic body is disposed so as to press the pressing member toward the accommodating portion,
Placing a cell group in the first area,
And monitoring the movement of the cell group located in the first area to the second area by removing the pressing member.
16. The method of claim 15,
Wherein the monitoring records an image of the cell group moving on the second area at a predetermined time interval through the imaging unit as an image.
16. The method of claim 15,
Wherein the second area is included on the patterning member received in the pressing member.
18. The method of claim 17,
Wherein the second area is located on a plurality of patterns each including a convex portion and a concave portion.
19. The method of claim 18,
Wherein the plurality of patterns include:
A pattern in which the widths of the convex portion and the concave portion are 5 micrometers and 5 micrometers respectively, the widths of the convex portion and the concave portion are 5 micrometers and 10 micrometers, respectively, And 20 micrometers, a pattern in which the widths of the convex and concave portions are 5 micrometers and 30 micrometers, respectively, and the widths of the convex and concave portions are 20 micrometers and 10 micrometers, respectively, Experimental method.
19. The method of claim 18,
Wherein the plurality of patterns are formed in a direction parallel to each other.
19. The method of claim 18,
Wherein the monitoring performs recording of a distance traveled per unit time along the plurality of patterns.
16. The method of claim 15,
Wherein the second area is an area where the pressing member presses the accommodating portion.
23. The method of claim 22,
The monitoring may include:
Wherein the pressing member is spaced apart from the second area so that an area occupied by the cell group per unit time of the second area is recorded as an image.
16. The method of claim 15,
Wherein the predetermined time interval is 5 minutes or longer.

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