KR20190050415A - Cosmetic composition containing extract of fraxinus rhynchophylla for improving skin wrinkle - Google Patents

Abstract

The present invention relates to a cosmetic composition containing, as an active ingredient, a subcritical extract of leaves and roots of Fraxinus rhynchophylla. The cosmetic composition of the present invention has an excellent effect of suppressing the formation of MMP-1 and an effect of synthesizing collagen, thereby reducing the skin wrinkles.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for improving skin wrinkles containing an ash tree extract as an active ingredient. BACKGROUND ART [0002]

The present invention relates to a cosmetic composition containing an extract of Fraxinus rhynchophylla obtained by extraction using a subcritical extraction method as an active ingredient, and more particularly to a cosmetic composition containing a subcritical extract of ash leaves and roots as an active ingredient, And more particularly to a cosmetic composition having excellent effects.

Skin acts as an important barrier to protect our bodies in both directions, preventing excessive loss of moisture from body fluids by evaporating body fluids and preventing the entry of harmful substances (irritances, microorganisms, allergens) from the outside. In addition, it is responsible for various physiological functions such as body temperature control, excretion, skin respiration, and selective absorption for specific substances.

Skin aging can be caused by active oxygen species activated by UV stress, disease state, environmental factors, wounds, and age. When active oxygen species are activated, lipids, proteins, polysaccharides and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging. Particularly, as the lipid composition and the content of the lipid layer constituting the skin barrier are changed, the function thereof is rapidly lowered. When the skin moisture content is decreased, the skin becomes dry, wrinkles are formed, stains, freckles, pigmentation, Lesions are induced.

On the other hand, MMP-1 (Matrix Metalloproteinase-1) plays an important role in skin aging process by ultraviolet rays. In skin exposed to ultraviolet rays, reactive oxygen species (ROS) are generated and the expression of MMP-1 is increased. As a result, components of collagen and elastin of skin tissue Degradation is intensified, which ultimately promotes skin aging.

 Recently, cosmetics using natural products have been developed to reduce the skin irritation caused by various chemical substances and the like, as the interest in appearance has increased. In addition to low adverse effects on skin, natural materials have recently become increasingly appreciated as a cosmetic raw material as consumers' response to cosmetics using natural materials has increased.

On the other hand, an organic solvent extraction method such as ethanol or methanol or a hot water extraction method is generally used as a method for extracting an effective component from a natural product. However, the extraction method using an organic solvent has a limitation in application due to the residual solvent, and the extraction method using hot water has a problem that the effective component is limited to the polar component or the active component is destroyed by the high temperature.

Korean Patent Laid-Open Publication No. 2013-0003171 discloses a skin whitening composition comprising a fermented product of an ash extract. Korean Patent Registration No. 10-1753687 discloses a skin whitening composition for anti-allergy and skin A composition for moisturizing is disclosed.

The present inventors have studied the applicability of various natural products to cosmetics. As a result, the present inventors tried to confirm the effect of improving skin wrinkles in subcritical extracts of ash leaves and roots.

The object of the present invention is to provide a cosmetic composition that exhibits excellent skin wrinkle-improving effects by using the extract obtained by extracting leaves and roots of Fraxinus rhynchophylla by subcritical extraction.

In order to achieve the above object, the present invention relates to an ash tree ( Fraxinus rhynchophylla ). The present invention provides a cosmetic composition for improving skin wrinkles.

The cosmetic composition of the present invention is characterized in that the subcritical extract of the ash tree is extracted for 10 to 50 minutes with water as a solvent at a temperature of 40 to 200 ° C and a subcritical state at a pressure of 1 to 122 atm.

Also preferably, the subcritical extract of the ash tree is an extract obtained from leaves or roots of an ash tree.

Preferably, the subcutaneous extract of the ash tree contains 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.

Also preferably, the subcritical extract of the ash tree is characterized by inhibiting MMP-1 expression and promoting collagen production.

The cosmetic composition may also be in the form of a solution, suspension, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, Lt; / RTI >

The cosmetic composition for improving skin wrinkles according to the present invention is characterized in that it contains leaves and a subcutaneous extract of ash tree having an effect of inhibiting MMP-1 production and collagen synthesis, which are superior to the extracts obtained by the conventional organic solvent extraction method or hot water extraction method, Therefore, it exerts excellent skin wrinkle improving effect.

1 is a graph showing the cell survival rate during treatment of the subcritical extract (Preparation Example 1) of the ash tree of the present invention and the 95% ethanol extract of the ash tree (Preparation Example 2).
FIG. 2 is a graph showing the inhibitory effect of MMP-1 on the subcritical extract (Preparation Example 1) of the ash tree of the present invention and the 95% ethanol extract of the ash tree (Preparation Example 2).
FIG. 3 is a graph showing the collagen biosynthesis effect of the subcritical extract (Preparation Example 1) of the ash tree of the present invention and the 95% ethanol extract of the ash tree (Preparation Example 2).

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. In addition, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention. The contents of all publications referred to herein are incorporated herein by reference. The term " drug " is used herein to refer to a reference quantity, level, value, number, frequency, percentage, dimension, size, quantity, Level, value, number, frequency, percentage, dimension, size, quantity, weight or length of a sample,

Throughout this specification, the words " comprising " and " comprising ", unless the context clearly requires otherwise, include the steps or components, or groups of steps or elements, And that they are not excluded.

The present invention relates to a cosmetic composition containing an extract of Fraxinus rhynchophylla obtained by extraction with a subcritical extraction method as an active ingredient.

The ash tree ( Fraxinus rhynchophylla ) used in the present invention is a deciduous tree of the ash tree growing on the foot of a mountain or valley. It is characterized by blooming and blooming, and it gets green when it puts branches into water, and it gets the name of ash tree. Depending on the locality, it is also called pong, orchid.

 The components of the ash tree include esculin, escoletin, and tannin. These antiinflammatory and antiinflammatory ingredients are known to alleviate the pain and anti-inflammatory action. Therefore, it is known that ash tree has a positive effect on gastrointestinal inflammation, asthma and bronchitis. It is said to be effective for eye diseases such as glaucoma, cataract and ocular hemorrhage because it is known to have a good effect on eye health by controlling the liver. In Oriental medicine, analgesic, anti-inflammatory and antipyretic effects are good, and it is used for the treatment of rheumatism, gout, enteritis and hepatitis.

The subcritical water extraction (SWE) used in the present invention is a method of producing subcritical water using an accelerated solvent extractor (ASE) or a pressurized liquid extraction (PLE) And then extracting it by using it. The present invention is characterized not only by extracting the active ingredient from the ash tree with excellent extraction efficiency by using the subcritical extraction method but also by increasing the content of the physiologically active ingredient contained in the ash tree extract. Water in the subcritical state is water at 40 to 200 ° C, more preferably 100 to 200 ° C and 1 atm (14.696 psi) to 122 atm (1800 psi), preferably 1 to 10 atm. The water in the critical state maintains a liquid state, not a gas. The subcritical extraction method is advantageous in that the extraction speed is faster than that of a general organic solvent extraction method, harmless to the environment, and high in extraction efficiency.

On the other hand, the cosmetic composition of the present invention can be used as an adjuvant commonly used in cosmetics such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, perfumes, fillers, ≪ / RTI > The amount of these various adjuvants is an amount customarily used in the art, for example, from 0.001 to 30% by weight based on the total weight of the composition. However, in any case, the adjuvants and their proportions may be chosen so as not to adversely affect the desired properties of the cosmetic composition according to the invention.

The formulation of the cosmetic composition for improving skin wrinkles of the present invention is not limited to a specific one, and examples thereof include a suspension, solution, emulsion, paste, gel, cream, lotion, powder, soap, A powder foundation, an emulsion foundation, a wax foundation, a pack, a massage cream, and a spray.

On the other hand, the cosmetic composition of the present invention can be used as an adjuvant commonly used in cosmetics such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, perfumes, fillers, ≪ / RTI > The amount of these various adjuvants is an amount customarily used in the art, for example, from 0.001 to 30% by weight based on the total weight of the composition. However, in any case, the adjuvants and their proportions may be chosen so as not to adversely affect the desired properties of the cosmetic composition according to the invention.

The formulation of the cosmetic composition for improving skin wrinkles of the present invention is not limited to a specific one, and examples thereof include a suspension, solution, emulsion, paste, gel, cream, lotion, powder, soap, A powder foundation, an emulsion foundation, a wax foundation, a pack, a massage cream, and a spray.

When the formulation of the cosmetic composition of the present invention is the above paste or gel, an animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide Can be used.

When the formulation of the cosmetic composition of the present invention is the powder or the spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component. Especially, in case of spray, Propellants such as hydrocarbons, propane / butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is the above solution or emulsion, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan can be used.

When the formulation of the cosmetic composition of the present invention is the suspension described above, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, and the like may be used.

When the formulation of the cosmetic composition of the present invention is the above-mentioned surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester.

In addition, the cosmetic composition according to the present invention preferably contains other ingredients such as ash leaves and root prime extract, which can give a synergistic effect to the main effect within a range not impairing the intended main effect of the present invention can do.

The cosmetic composition of the present invention may be used alone or in combination, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and may be used in a different number of times depending on the skin condition or taste of the user.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

Manufacturing example  1: Ash tree Subcritical extract  Produce

Ash tree ( Fraxinus rhynchophylla leaves and roots were washed with purified water, dried and pulverized, and then crushed to a certain size using a 10 mesh sieve. Then, the asyle leaves and the ground pulverized material were extracted with 1 L of purified water for 30 minutes at 150 캜 under a subcritical extraction condition of 2 atm, and then filtered with a filter of whatman # 3. After filtration, the filtrate was concentrated under reduced pressure at 50 DEG C or below, lyophilized, and dissolved in 50% (w / v) butyleneglycol to prepare an extract.

The extracts obtained by extracting the ash leaves and roots in the subcritical extraction condition as described above were referred to as the subcritical extract of the ash tree and then used in the following experiment.

Manufacturing example  2: Preparation of 95% ethanol extract of ash tree

The leaves and roots of the dried ash tree were both pulverized, and then 95% ethanol was added to make 150 g / L. Thereafter, the mixture was allowed to stand at room temperature for 48 hours, filtered through whatman # 3 filter paper, concentrated under reduced pressure at 50 ° C or below and lyophilized.

Experimental Example  1: ash Subcritical extract  Evaluation of cell survival rate

In this Experimental Example, the toxicity of the sub-critical extract of Ashira (Preparation Example 1) of the present invention to cells was measured.

The cytotoxicity was determined by modifying the 'Mosmann' method for measuring cell viability using 3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide (MTT) (HDFn, Human Dermal Fibroblast neonatal) was dispensed into a 96-well plate at a concentration of 2 × 10 ⁴ cells / well and cultured at 37 ° C. and 5% CO ₂ for 24 hours. The culture medium was removed and the extracts obtained in Preparation Example 1 and Preparation Example 2 were cultured for 24 hours in media treated at 10, 30, and 100 占 퐂 / ml, respectively. Then, 30 μL of the MTT reagent dissolved at 5 mg / mL was added and cultured at 37 ° C. and 5% CO ₂ for 2 hours. 100 μL of DMSO was added per well, and the mixture was stirred for 10 minutes and absorbance was measured at 540 nm.

As a result of the experiment, it was confirmed that the cytotoxicity was not observed up to 100 占 퐂 / ml in Production Examples 1 and 2. From this, it was confirmed that the subtilty extract of the present invention is harmless to the human body and excellent in safety (Fig. 1).

Experimental Example  2: ash Subcritical extract MMP -1 generation inhibition evaluation

In this Experimental Example, the effect of inhibiting the production of MMP-1 in the sub-critical extract of Ashira (Preparation Example 1) of the present invention was examined.

Human skin fibroblasts were divided into 24-well plates (Nunc, Denmark) at a concentration of 1 × 10 ⁵ cells / well and cultured at 37 ° C. and 5% CO ₂ for 24 hours. After washing twice with phosphate buffered saline (PBS), the cells were incubated with a physiological saline solution at a rate of 6 J / cm ² Of UV light. The supranuclear mustard extract of Preparation Example 1 and the ash ethanol extract of Preparation Example 2 were cultured in DMEM medium supplemented with 2% FBS and 2% DMEM medium containing no ash extract for 48 hours as a control.

After culturing, the supernatant of each well was collected and the amount (ng / ml) of the newly synthesized MMP-1 was measured using an MMP-1 assay kit (R & D system, USA) The percent inhibition (%) was calculated and the results are shown in FIG. 2 and Table 1.

Name of sample Treatment concentration
(ppm) Inhibition rate of MMP-1 production (%) Production Example 1 100 46.50 30 30.77 10 22.00 Production Example 2 100 29.87 30 18.26 10 14.47 Adenosine 100 44.34

As a result of the experiment, the supranuclear limulus extract (Preparation Example 1) of the present invention was superior to the ethanol extract of ash tree (Preparation Example 2) in inhibiting the production of MMP-1, The subcritical extract of the ash tree of the present invention had higher efficacy at the same concentration (100 ppm) compared to the adenosine of the present invention.

The suppression rate of MMP-1 production at the level of the extract obtained by mixing various components means that the subcritical extract of the ash tree of the present invention exhibits excellent skin wrinkle-reducing effect.

Experimental Example  3: ash Subcritical extract  Evaluation of collagen biosynthesis

In this Experimental Example, collagen biosynthesis effect of the sub-critical extract of Ashira (Preparation Example 1) of the present invention was confirmed.

Human skin fibroblasts were divided into 24-well plates (Nunc, Denmark) at a concentration of 1 × 10 ⁵ cells / well and cultured at 37 ° C. and 5% CO ₂ for 24 hours. The ash supercritical extract of Preparation Example 1 and the ash ethanol extract of Preparation Example 2 were cultured in DMEM medium without FBS and DMEM medium containing no ash extract as a control for 24 hours.

After the incubation, the supernatant of each well was collected and the amount (ng / ml) of synthesized collagen was measured using Procollagen type I C-peptide enzyme immunoassay (EIA) kit (Takara, Japan) The collagen synthesis rate (%) was calculated according to the results, and the results are shown in FIG. 3 and Table 2.

Name of sample Treatment concentration
(ppm) Collagen synthesis rate (%) Production Example 1 100 136.62 30 126.78 10 116.56 Production Example 2 100 124.97 30 119.74 10 111.42 TGF-beta 200 134.28

As a result of the experiment, the supranuclear essential oil extract (Preparation Example 1) of the present invention was superior to the ash tree ethanol extract (Preparation Example 2) in the collagen production efficiency, and at the level of the extract containing various kinds of substances, Collagen synthesis ratio of the present invention means that the sub-criticality extract of the present invention exhibits excellent collagen synthesis effect.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (6)

Ash tree ( Fraxinus rhynchophylla ). < / RTI > [Claim 2] The method according to claim 1, wherein the subcutaneous extract of the ash tree is extracted from the ash tree for 10 to 50 minutes at a temperature of 40 to 200 [deg.] C and a subcritical water at a pressure of 1 to 122 atm. A cosmetic composition for improving wrinkles. The cosmetic composition for improving skin wrinkles according to claim 1, wherein the subcritical extract of the ash tree is extracted from leaves and roots of an ash tree. The cosmetic composition for improving skin wrinkles according to claim 1, wherein the subcutaneous extract of the ash tree is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. The cosmetic composition for improving skin wrinkles according to claim 1, wherein the subcutaneous extract of the ash tree inhibits MMP-1 production and promotes collagen production. The cosmetic composition of claim 1, wherein the cosmetic composition is selected from the group consisting of solutions, suspensions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations and sprays Wherein the cosmetic composition has a formulation.

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