KR20190017585A - functional hygienic goods and their preparation method - Google Patents

Abstract

The present invention provides a functional hygienic article containing a functional substance using a needleleaf extract and a manufacturing method thereof. Thus, the present invention is capable of relieving skin trouble.

Description

TECHNICAL FIELD The present invention relates to a functional hygiene article containing a functional material using a softwood extract,

The present invention relates to a functional hygiene article containing a functional material for reducing antibacterial activity, anti-allergy, anti-inflammatory activity, and odor, and a method for producing the functional hygiene article. More particularly, the present invention relates to a functional hygiene article comprising a by- The present invention relates to a sanitary napkin, a mask, a pad for preventing bedsore, an atopic panty liner, a wound dressing, and a wet tissue using the functional sanitary article.

Textile fabrics such as sanitary napkins, diapers, incontinence pads, etc. are a kind of absorbent products that need to be used continuously for a certain period of time by wearing the same products for a certain period of time for personal hygiene and mainly used disposable products do.

Such a fiber fabric usually includes an absorbent body having a liquid such as menstrual blood or urine, and a liquid-impermeable sheet for preventing the liquid from leaking out and smudging the clothes of the user is applied as an essential constitution. The above-mentioned textile fabric is generally used to come in contact with the skin. Since the absorbent body absorbs menstrual blood or urine and retains it for a long period of time, it becomes an environment favorable for propagation of various bacteria. In addition, unpleasant odor may be generated due to menses stored in the absorber or urine itself or due to bacteria that reproduce here.

For this reason, it is necessary to maintain cleanliness when using an absorbent fiber fabric, and it is necessary to prevent the propagation of bacteria that may cause diseases such as eczema and vaginitis, as well as removal of odor. However, if a synthetic synthetic disinfectant or preservative is applied to this, a synthetic bactericide or an antiseptic ingredient may be absorbed into the human body due to the nature of the product being directly contacted with the skin, and all the microorganisms beneficial to the human body may be killed, And side effects such as trouble can be caused by the nature of products that are worn for a long time, this side effect can occur frequently or may be worse.

Various sanitary articles have been developed to solve these problems, and Korean Patent Laid-Open Publication No. 2003-0068066 discloses a method for manufacturing natural antibacterial female sanitary articles coated with a gardenia extract.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems and it is an object of the present invention to provide a sanitary napkin, a mask, a pad for preventing bedsore, and an atopic panty liner to which a functional material including a chip leaf extract is applied. And a functional hygiene article for reducing odor, and a method of manufacturing the functional hygiene article.

The present invention provides a functional hygiene article containing a functional material using a softwood extract and a method of manufacturing the functional hygiene article.

According to the present invention, since the softwood extract used in the present invention is a natural substance, the use of a chemical additive for the addition of fragrance or germicidal bleaching and the like is excluded, so that the hygienic product containing the same is non-irritating And there is an improved effect of eliminating concerns such as skin troubles.

The PHYTONCIDE contained in the softwood extract of the present invention is a substance secreted by plants to resist pathogens, insects and fungi, and has a stress hormone reduction effect, strong antibacterial activity, formaldehyde removal effect, and house dust mite growth inhibition effect. Phytoncide solves the stress, strengthens the bowel and cardiopulmonary function, and sterilization also occurs. In addition, it is effective in removing odor from germs.

In addition, since the functional material contained in the functional hygiene article of the present invention contains a functional ingredient such as terpenes contained in the by-product of the conifers, it has antibacterial activity, anti-allergy, anti-inflammatory activity and deodorizing effect.

Further, since the present invention utilizes coniferous byproducts, it is economically advantageous and can reduce environmental pollution.

Figure 1 is a photograph of the organic carrier used in the present invention.
2 is a photograph of a film using a softwood extract.
3 is a photograph of a microcapsule solution using a softwood extract.
4 is a microcapsulation SEM photograph using a softwood extract.
5 is a front view of the mask of the present invention.
6 is a front and side view of the sanitary napkin of the present invention.
7 is a front and side view of the pantiliner of the present invention.
8 is a photograph of a wet tissue according to the present invention.
FIG. 9 is a photograph showing the comparison of the numbers of bacteria (a) at 0 hour and (b) at 24 hours after inoculation of E. coli into a sample and then dropping the pine tree extract.
FIG. 10 is a photograph showing the comparison of the numbers of bacteria (a) at 0 hour and (b) at 24 hours after inoculation of P. aeruginosa into a sample.
Fig. 11 is a photograph showing the comparison of the numbers of bacteria (a) at 0 hour and (b) at 24 hours after inoculation of pneumococci into a sample and then dropping the pine tree extract.
12 is a photograph of the fungus 28 days after the sample is inoculated.
13 is a standard curve graph using CFU / ml concentration of SA bacteria and OD value of an ELISA reader.
14 is a standard curve graph using CFU / ml concentration of SA bacteria and OD value of a standardphotometer.

Hereinafter, the present invention will be described in more detail with reference to embodiments thereof. Prior to the description of the present invention, the following specific structure or functional description is merely illustrative for the purpose of describing an embodiment according to the concept of the present invention, and embodiments according to the concept of the present invention may be embodied in various forms , And should not be construed as limited to the embodiments described herein.

The embodiments according to the concept of the present invention can be variously modified and can take various forms, so specific embodiments are described in detail herein. However, it should be understood that the embodiments according to the concept of the present invention are not intended to limit the present invention to specific modes of operation, but include all modifications, equivalents and alternatives falling within the spirit and scope of the present invention.

The present invention provides a functional hygiene article containing a functional material using a softwood extract.

The functional hygiene article of the present invention may specifically be a mask, a sanitary napkin, a bed preventing pad, an atopic panty liner, a wet tissue, or the like. The functional hygiene article of the present invention is effective for antibacterial and deodorization when used in diseases such as atopy, wound dressing, candida, and pressure ulcer.

In addition, the functional material of the present invention can be obtained by treating the mature pine needle and the immature pine needle of a conifer with an extract of a softwood and a water-soluble extract, a microcapsule solution of a conifer-extract, an organic carrier containing a conifer-extract, a microcapsule solution of a conifer- A material prepared by processing a film, a organic carrier obtained by pulverizing a conifer-extracted microcapsule solution, or the like.

The softwood extract of the present invention is extracted using coniferous trees such as pine, pine, perilla, and cedar. In order to extract the chip leaf water extract of the present invention, it is preferable to use coniferous by-products and immature conifers of the conifers, in particular, poultry by-products, mature pods and immature pods which are generated after the pods are dislodged.

In Korea, rice husk byproducts are incinerated because they can be left as environmental pollutants by neglecting to nature as waste without special use. Therefore, the use of the above-mentioned zizyparias by-products and immature zizyphi is economical and environment-friendly.

The rice husk by-products used in the present invention may be in a wide variety of forms depending on the breaking-out condition of pine nuts, the pine nut producing area, the storage state after breaking off, and the like. The amount of residual softwood extract components differs depending on the difference in appearance, which affects the deviation of the yield of the chip leaf extract to be extracted under the same distillation processing conditions. Therefore, in order to determine the optimal extraction process conditions that can be reproducible, it is necessary to obtain as many reliable results as possible by extracting as many times as possible a large amount of rice husk by-products as many times as possible. Therefore, in the present invention, the pine nut is disassembled, and then the 28% moisture content of the dried zipper by-products is shaded and adjusted to a size of 2 to 4 cm with a moisture content of 20%. In each distillation condition, The extraction behavior of softwood was investigated by extraction experiment three to twelve times.

The preparation of the conifer extract solution of the present invention is as follows.

After drying in the shade, weigh 1 ~ 2 kg of pomace by-products prepared in 2 ~ 4cm size into 5L round flask. Separate steam generator was installed to maintain the distillation temperature at 100 ± 3 ℃ and steam was generated directly through the lower part of the sample. The extraction time was varied from 10 min to 10 min intervals and distilled for a certain time. The reproducibility was observed by repeatedly proceeding 3 to 12 times per condition.

The solution obtained by distillation is separated from the oil phase and the water phase by the specific gravity difference. The supernatant is pine wood phytoncide oil (essential oil), and the lower phase water phase is hydrosol (hereinafter referred to as 'shredded product') containing a water soluble softwood extract component. )to be.

The conifer extract solution of the present invention may contain the above essential oils and / or water-soluble substances.

The process for preparing the soft-bark extract microcapsules used in the present invention is as follows.

A first stage water retention manufacturing step;

The first step is to prepare an aqueous solution of chitosan. Since the chitosan is not dissolved in an aqueous solution of neutral or basic atmospheres, it must be dissolved in an aqueous solution of an acidic atmosphere. The type of acid used to make the acidic aqueous solution is not particularly limited, It is only necessary to select the type thereof, preferably acetic acid. The acidic aqueous solution preferably has a pH of 2 to 4. The concentration of the chitosan solution is required to form chitosan microcapsules for the sustained release of chitosan by using acetic acid as a factor affecting the capsule thickness in the encapsulation process. Therefore, a thick capsule wall is required, and 2 to 4 wt% desirable. The concentration of chitosan is most preferably 2 to 5%. Chitosan is added to an acidic aqueous solution and stirred to prepare an aqueous chitosan solution. The amount of the chitosan is preferably 10 to 30% by weight, more preferably 20% by weight.

Chitosan (β (1-4) -2amino-2-deoxy-D-glucose is a derivative obtained by deacetylation of chitin (β (1-4) -2-acetamide-2-deoxy-D-glucose). It is insoluble in water and organic solvents but dissolves in acidic pH below 6.5. Chitosan is a non-toxic, biocompatible, biodegradable polymer that is known to improve wound healing.

The second step is to dissolve the surfactant in the chitosan aqueous solution to increase the dispersibility of the softwood extract. The type of the surfactant used in the process is not particularly limited, but Tween 80 or Tween 20 can be used. When the Tween 80 is used alone, the dispersing force may not be sufficient and the liquid phase may be gelled as a whole. Therefore, Span 80 is preferably mixed to maximize the dispersing power. Tween 80 and Span 80 are mixed to obtain a capsule liquid in which a homogeneous phase is retained for 1 month or more. The optimal amount of Tween 80 and Span 80, which are two surfactants, is 1 to 3% by weight, preferably 2 Weight%. The third step is a step of dispersing the softwood extract in the aqueous solution of chitosan in which the surfactant is sufficiently dissolved. The aqueous solution of chitosan is stirred at a rpm of 7,000 to 10,000, preferably at a rpm of 9,000, and a softwood extract is added to the chitosan aqueous solution . The stirring speed shown is an example, and the stirring speed is not particularly limited.

A second stage polyphase emulsion preparation step;

The first step is to add a surfactant to the oil phase to dissolve the oil phase. Preferably, the oil phase and the surfactant are mixed at a weight ratio of 1:10. The liquid phase and the surfactant in the oil phase are not particularly limited, and a kind suitable for the application is used.

 The second step is to stir the liquid phase of the oil in which the surfactant is fully dissolved at a rpm of 7,000 to 10,000, preferably at rpm of 9,000, and add the distilled water prepared in the first step. The stirring speed shown is an example, and the stirring speed is not particularly limited.

The kind of the surfactant is not particularly limited, but Span 80, Span 20 and Mineral Oil can be used, and it is preferable to use Span 80 and mineral oil. Tween-based surfactants used in the manufacture of water-in-oil can not be used. It is preferable to use a Span-type surfactant, which is a surfactant used in a water phase, because the viscosity of the water-soluble oil to be dispersed on the oil is high due to the viscosity of the chitosan solution and the effect of the Tween series surfactant. If the surfactant used in the water-in-oil type is used, the finished capsule liquid is separated from the liquid phase, so that not only the phase stability is poor, but also the cross-linking agent is not properly mixed. Therefore, it is preferable to use a Span series surfactant used for water phase. Considering the viscosity of the vigorous oil, the Span 80 is preferably used in an amount of 10 to 30 g, more preferably 10 g. The amount of mineral oil is preferably 50 to 200 g, more preferably 100 g.

A third step of preparing an aqueous solution containing TPP;

It is preferred that the TPP aqueous solution is a slightly acidic, neutral or weak base. If the acidity of the TPP aqueous solution is strong, it may adversely affect the collected softwood extract, and if it is strongly basic, it may adversely affect encapsulation. The TPP content in the aqueous TPP solution is not particularly limited. The TPP concentration is preferably 3% or more, and more preferably 5% or less. If the concentration of TPP is less than 3%, the crosslinking is not properly performed, which adversely affects the encapsulation. If the concentration exceeds 5%, the crosslinking rate of chitosan becomes too fast to adversely affect encapsulation.

The addition amount of TPP is preferably 10 to 15% by weight, more preferably 15% by weight of TPP because 15% by weight of the TPP solution is added and the liquid phase is uniform and fluidity is increased. In this step, similarly to the above step, when TPP is added, TPP must be dispersed in the liquid before the crosslinking takes place, so TPP should be injected during stirring of the mixed solution. If the stirring speed is too low at this stage, the chitosan ion in the aqueous solution of chitosan is crosslinked before the TPP is dispersed, so that the crosslinking of the capsule does not occur. Therefore, the crosslinking of the capsules can be achieved only by stirring at a stirring speed higher than a certain level. In order to homogenize the TPP concentration, the cross-linking agent is slowly added to the TPP solution in a droplet method for 30 minutes, followed by an additional crosslinking reaction for 30 minutes.

Step 4: contacting the polyphase emulsion prepared in the second step with the TPP aqueous solution prepared in the third step to conduct the reaction;

The polyphase emulsion prepared in the second step is stirred at rpm of 7,000 to 10,000, preferably at rpm of 9,000, and the TPP aqueous solution prepared in the third step is slowly added by a droplet method for 30 minutes to prepare soft-bite extract microcapsules. The stirring speed shown is an example, and the stirring speed is not particularly limited.

The size of the soft-bore extract microcapsules is preferably 10-300 m. If it is more than 10 탆, the capsule wall becomes thick and the release of the core material becomes difficult, so that the initial release amount is small, and the deodorizing and antibacterial effect are insufficient. Also, since the amount of the core material is decreased and the discharge amount is decreased, If it is less than 300 m, the wall of the microcapsule becomes thin, and the capsule is broken easily or the initial release amount is increased. After a long time, the remaining core material is not removed and the clear effect can not be obtained.

Gelatin instead of chitosan may be applied to the microcapsules of the present invention.

In addition, the microcapsule constituting material of the present invention is characterized by being a terephthalate derivative, gum arabic, gelatin, collagen, CMC derivative. By using this, the efficacy of the liquid phase inside the microcapsule can be maintained for a long time.

In addition, the core material of the present invention is characterized by containing terpenes extracted from matured pine nuts and immature pomace buds after being extracted from pine nuts such as coniferous pine, pine, cedar, and Japanese white pine.

In order to apply the microcapsule solution to a sanitary article, for example, when mixed with a textile fabric, 0.1 to 15 parts by mass of a microcapsule solution may be adhered to 100 parts by mass of the fabric.

The sanitary article of the present invention may also contain a binder resin, which is preferably a water-soluble polymeric binder resin. The binder resin mainly serves to fix the microcapsules of the present invention to the textile fabric. The amount of the binder resin to be adhered to the fiber fabric is preferably 0.05 to 30 parts by mass with respect to 100 parts by mass of the fiber fabric. Further, the binder resin is characterized in that it is fixed in a mesh shape to the fiber fabric.

In addition, the microcapsules of the present invention can be applied to fabrics, synthetic fibers, pulp, synthetic resin materials, etc., and made into films to be applied to functional hygiene products.

The film may be specifically a PVA film.

In addition, the present invention can be applied to a functional hygiene article as an organic carrier by powdering the microcapsule solution.

Examples of the organic carrier include dextrin, SAP, aerogels, hydrophobic silicates, silicic acid-based materials such as zeolite and elvan, which are inorganic porous materials, and photocatalysts such as TiO2 and ZnO2.

The organic carrier of the present invention can be prepared by preparing the microcapsule solution of softwood extract by powder working through a fluidized bed granulator or a mixer and adding it to materials such as nonwoven fabric, pulp and woven fabric.

The hydrophobic inorganic porous material (organic carrier) to which the microcapsule of the present invention is adhered may be mixed with the fiber fabric. In this case, it is preferable that 0.1 to 15 parts by mass of the hydrophobic inorganic porous material is attached to 100 parts by mass of the fabric.

In addition, the binder resin may be mixed together. In this case, it is preferable that the binder resin contains 0.05 to 30 parts by mass of the binder resin with respect to 100 parts by mass of the fiber fabric.

Hereinafter, a functional hygiene product including a functional material using the softwood extract of the present invention will be described with reference to examples.

Manufacture of microcapsules of softwood extract

The functional material of the present invention may be a microcapsule containing a softwood extract. The microcapsule production method is as follows.

0.5 g of Span, 100 g of gum arabic and distilled water are added to a 1 L beaker to prepare a first solution. At this time, the solution is made to be 500 g in total. When the above solution is completely dissolved and the diluted solution is completed, 33.3 g of the conifer extract is added and stirred at 8,000 rpm for 10 minutes using a homogenizer.

Separately, 250 g of ethanol was slowly added to 500 g of the castor oil, and the mixture was stirred for 10 minutes at a rpm of 8,000 using a homogenizer to prepare a secondary solution.

The second solution is slowly added to the first solution while rotating the homogenizer at 8,000 rpm. After stirring for 10 minutes, 2 L of anhydrous alcohol is added, and the mixture is stirred at 8,000 rpm for 10 minutes to complete the soft capsule extract microcapsule.

Containers containing softwood extract carrier  Produce

Wanwimol and others were modified to perform Phy encapsulation. After completely dissolving 1 g of 11 CH in a concentration of 0.1% acetic acid, 10 g of MD is added to prepare a solution. 20 g of Phy was added to the mixed solution of CH / MD and 1.5 g of Tween 80 was added thereto and emulsified at 7500 rpm for 20 minutes with a homogenizer (Ika, T25 basic homogenizer). The emulsified solution was dispersed in 0.1 M NaOH solution (1 mL / 100 mL) to solidify the CH. The solidified particles were centrifuged, filtered and washed with distilled water, and then lyophilized for 24 to 48 hours to be pulverized. When the hydrogel was added, the Phy capsules were added at a concentration of 1%, respectively.

Manufacture of films containing conifer extracts

For the production of softwood extracts, frozen and thawed were repeated three times. PVA and PET were put into 100 ml of distilled water at a ratio of 5: 5, mixed with a homogenizer (12500 rpm) for 10 minutes, and then dissolved in an autoclave (121 ° C, 20 minutes). The bubbles were removed by placing them in a measuring cylinder to remove bubbles in the dissolved PVA: PET composition. 15 g of each petridish was added to the PVA: PET solution without bubbles and the control group without the Phy capsules and the Phy capsules were added. The mixture was left at -80 ° C for 4 hours and thawed at room temperature for 8 hours. A total of three treatments were conducted to form a softwood extract film.

Example  One

The thus obtained soft-leaf extract microcapsules were mixed with a conifer-extracted microcapsule at a rpm of 8000 in an agitator for 10 minutes so as to obtain a titanium dioxide powder of 1% by weight, thereby preparing a conifer-extracted extract microcapsule mixed with titanium dioxide. This is sprayed on the fabric of the sanitary napkin in an amount of 5% by weight relative to the entire sanitary napkin and dried.

Comparative Example

Use a sanitary napkin that does not contain spraying or mixing softwood microcapsules.

Experimental Example  One

In order to confirm whether the mixed cloth, non-woven fabric, pulp or the like mixed with softwood extract microcapsules has a deodorizing activity against a packed sanitary napkin, the present invention is applied to a sanitary napkin with a thickness of 50 mm (L) * 300 mm (w) * 250 mm The concentration of ammonia, methyl mercaptan, dimethyl sulfide, and dimethyl disulfide with time was investigated using an acrylic reactor.

The initial concentrations of ammonia, methyl mercaptan, dimethyl sulfide, and dimethyl disulfide as test gases were respectively 1440 ppb, 0.62 ppb, 13.21 ppb, and 1.53 ppb, respectively, in the comparative example and the example 1, To 60%, and the concentration of the test gas was measured at the initial (0 minute) 2 days, 5 days, 7 days and 10 days, respectively. Ammonia as a test gas was measured by PhoCheck VOCMETER, methyl mercaptan and dimethyl sulfide, and dimethyl disulfide by GC-MSD. The results are shown in Tables 1 to 4.

The removal rate of test gas for each hourly period was calculated by the following equation. Test gas removal rate (%) = [{blank concentration- (sample concentration)} / (blank concentration)] * 100.

As a result, the ammonia, methyl mercaptan, dimethyl sulfide, and dimethyl disulfide concentrations were hardly changed in the comparative examples. In the examples, it was found that ammonia and methyl mercaptan concentrations measured after 10 days were removed to an undetectable level. The dimethyl sulfide concentration was decreased by 88% and the dimethyl disulfide concentration was decreased by 84%.

These results show that the odor deodorization efficiency of the functional hygiene article of the present invention is excellent because the odor can be deodorized within a shorter time than the previous invention.

Experimental Example  2

In order to confirm whether the sanitary napkin sprayed with the chip leaf extract microcapsule solution had deodorizing activity, the present invention was carried out as follows.

The sanitary napkin of Example 1 and the nonsprayed sanitary napkin sprayed with a mixture of TiO 2 and softwood extract microcapsules of the present invention were sprayed onto the sanitary napkin of 50 mm (L) * 300 mm (w) * 250 mm And the deodorizing action after 5 days was confirmed by forcibly injecting ammonia, methyl mercaptan, dimethyl sulfide, and dimethyl disulfide as odor causing substances into the respective reaction vessels provided with the sanitary napkin. The results are shown in Table 5 below.

Experimental Example  3

Antibacterial tests were carried out on Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538 and Klebsiella pneumoniae ATCC 4352 according to the method of KCL-FIR-2001: 2011, And the rate of bacterial reduction after the passage of time was observed.

Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, and Klebsiella pneumoniae ATCC 4352) were inoculated on the sample, and then the pine tree extract was dropped in the center and exposed for 24 hours After which the rate of bacterial reduction is measured.

In the case of Escherichia coli, bacterial counts were increased after 24 hours in BLANK, and bacterial counts decreased by 99.8% after 24 hours.

10 and Table 6, the bacterial counts of BLANK were increased after 24 hours, and the pine tree extract solution was decreased by 99.8% after 24 hours.

In the case of pneumococci, bacterial numbers were increased after 24 hours in BLANK and pine bark extract solution was decreased by 99.8% after 24 hours.

(2) Antifungal test

An antifungal test was conducted on the conifer extract. As a test method, ASTM G 21: 2013 was used for testing. Aspergillus niger ATCC 9642, Penicillium pneumophilum ATCC 11797, Chaetomium globosum ATCC 6205, Gliocladiumvirens ATCC 9645 and Aureobasidium pullulans ATCC 15233 were used as the mixed fungi.

First, five molds are prepared at high concentration in each spore suspension, and then poured into a sterile culture dish. The antimicrobially treated test specimen is placed on the surface of the agar agar medium. Spray composite spore suspension onto agar and test specimen surface. The inoculated antimicrobial treatment test sample, positive control (antifungal growth inhibitor) and negative control (non-antimicrobial antimicrobial agar medium) control are incubated for 28 days at 90% relative humidity or higher. The following readings were used to read the results.

0: No growth of mycelium was observed in the inoculated portion of the test specimen.

1: The area of mycelial growth area recognized in the inoculated part of the test specimen is less than 10% of the whole area.

2: The area of mycelial growth area recognized in the inoculated part of test specimen is 10 ~ 30% of the whole area.

3: The area of mycelial growth area recognized in the inoculated part of the test piece is 30 ~ 60% of the whole area.

4: The area of mycelial growth area recognized in the inoculated part of the test specimen is 60% or more of the whole area.

As a result, mycelial growth was not recognized in the inoculated portion of the test piece for 4 weeks during the culture test period.

Experimental Example 4- Atopic effect improvement experiment

(1) Titer change of IgY antibody in the product according to addition level of pine tree extract

0.3%, 0.5%, 0.8%, 1.0%, and 1.3%, respectively, in order to investigate the effect of pine wood extract (Phytoncide, PC) on the activity level of IgY antibody in the product. , 1.5%, 1.8%, and 2.0%, respectively, and the anti-atopic / therapeutic efficacy was evaluated by measuring the IgY titers by the concentration of the additive substances by ELISA (Kim, et al., 1999).

In order to investigate whether or not it occurred.

The binding capacity of IgY antibody to the SEB antigen was determined by preparing a certain concentration of SEB toxin secreted by the SA culture, reacting the mixture at a predetermined level for each level of the control and PC, and then measuring the binding capacity of the SEB antigen Antibody binding ability was examined.

As shown in Table 9, the SEB concentration of the SA medium was (0.240 ± 0.021), and the SEB concentration was 0.195 ± 0.015, which was significantly lower than that of the SA medium (p <0.001). The SEB concentration of PC was 0.169, 0.187, 0.185, and 0.192 at 0.5%, 1.0%, 1.5% and 2.0%, respectively, which were significantly higher than those of SE culture medium (p <0.001). There was no significant difference in SEB concentration of PC (experimental group) by PC addition level, and SEB concentration did not decrease proportionally with increasing level of PC.

On the other hand, the SEB concentration of the control group and the PC group (experimental group) was lower than that of the control group. Although the difference in the concentration between the two groups was not significant, the PC 0.5% was significantly lower than the control group (p <0.05). The efficiency (%) of antigen binding ability to SEB was about 5.1% higher in the PC group (23.6%) than in the control group (18.5%).

The results of this experiment are summarized as follows: The antigen binding ability of PC group (23.6%) was about 5% higher than that of the control group (18.5%). Although the statistical significance was not recognized, the possibility that PC-added products (0.5% -1.0%) would improve the binding ability of antigen antibody is highly likely. However, there was no significant difference in antibody binding capacity of PC to SEB antigen by PC addition level, and there was not a proportional increase of SEB binding ability with increasing level of addition.

(3) SA bacteria  Neutralization reaction test

In order to investigate whether pine needle extract (PC) effectively neutralizes bacterial toxicity due to sterilization (antibacterial action) against intact SA bacteria and secondary neutralization of IgY antibody against SEB antigen in the body, PC The neutralizing (binding) ability of SA microorganisms of PC was measured by indirect ELISA method.

For the test measurement, SA was cultured and the concentration of the bacteria was adjusted to 1 × ^{10 9} CFU / ml. After the heat treatment, the culture was reacted according to the level of the control and the PC, and the OD was measured using the indirect ELISA technique. Regression equations were obtained by using the correlation between the CFU / ml concentration of SA bacteria and the OD value of the spectrophotometer and the ELISA reader, using the Agar plate medium, and then calculating the standard curve (CFU / ml) Respectively. This is shown in Figures 13 and 14.

The standard curves shown in FIGS. 13 and 14 are regression line linear equations that can be used to measure the viable cell count (CFU / ml) of Staphylococcus aureus bacteria using a spectrophotometer or an ELISA reader. 0.98) is very high and can be very useful for measuring the binding ability of an antibody against a specific antigenic bacterium. In particular, this method was used in the experiment using this method as a useful method for predicting the results of animal clinical trials in combination with the indirect ELISA method.

Number of viable cells of the SA culture stock solution as shown in Table 10 is 5.425 x 10 ⁸ was, after the control and the number of viable cells is quite reaction were significantly lower (67.1%) than the SA culture stock solution as ^{x10 8 3.642 (p <0.013)} . Number of viable cells of the PC group are each 2.835 x 10 eseo 0.5% by the addition levels of PC, 1.0%, 1.5%, 2.0% 8, 3.391 x 10 8, 3.375 x 10 8, 3.282 x 10 8 as the number of viable cells of the SA culture stock solution (P <0.001), respectively. There was no significant difference in the number of viable cells of PC by PC addition level, and the proportion of viable cells was not decreased as the level of addition was increased.

On the other hand, the reduction rate (%) due to the neutralization power of IgY antibody against Staphylococcus aureus in the control group and the PC group was higher than that in the control group, and the ability to bind with SA bacteria was found to be good. Although the difference between the two groups was not significant, the number of viable cells in the PC 0.5% group showed a better binding ability (77.8%, p <0.06) than that of the control group alone. The ability of the PC group to reduce the SA bacteria was 52.3% - 62.5%, which was higher than that of the control group (67.1%) and the average level was about 6.9% -22.2%. However, there was no significant difference in the antibody binding capacity of PC to SA bacterium by PC addition level, and there was not a proportional increase in SA binding ability as the level of addition increased.

The results of this experiment are summarized as follows: When pine tree extract (PC) was added to the control group, the neutralization ability due to the binding with Staphylococcus aureus (SA) was enhanced, which was higher than that of the control group alone. Although there was no statistical significance between them, the possibility that the products added with 0.5% -1.0% of PC level would have an improvement effect on the antibody binding ability is very high.

Claims (5)

Characterized in that 0.1 to 15 parts by mass of the conifer-extracted microcapsule solution is mixed with 100 parts by mass of the fabric or film, and 0.05 to 30 parts by mass of the binder is mixed with the functional material. [2] The functional hygiene article according to claim 1, wherein the softwood extract is extracted from at least one selected from the group consisting of Pinus koraiensis, Pinus densiflora, Pinus densiflora, and Cedarwood, and an immature oropharyngeal. The functional hygiene article according to claim 1, wherein the microcapsule solution is powdered and mixed with an organic carrier. Preparing softwood extract microcapsules; The method comprising mixing 0.1 to 15 parts by mass of the soft-bore extract microcapsule solution with 100 parts by mass of the fabric or film, and mixing the binder with 0.05 to 30 parts by mass of the functional material using the softwood extract. Method of manufacturing functional sanitary ware. The method of claim 4, wherein the softwood extract microcapsule comprises the steps of: Preparing a polyphase emulsion by mixing a surfactant and an oil with water; Preparing a TPP aqueous solution; Mixing the polyphase emulsion and the TPP aqueous solution; Wherein the method comprises the steps of: preparing a functional material containing a functional ingredient using a conifer extract;

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