KR20180013603A - Alcohol detoxification composition comprising extract of natural herb - Google Patents

Abstract

The present invention relates to a hangover relief composition comprising an extract of natural herbs and, more specifically, to a hangover relief composition comprising an extract of complex herbs consisting of 20 to 30 parts by weight of a hot water extract of green tea seeds, 40 to 60 parts by weight of a hot water extract of Houttuyniae Herba and 20 to 30 parts by weight of a hot water extract of lotus leaves as an active ingredient, thereby having excellent antioxidant activity, promoting alcohol dehydrogenase activity and being effective in preventing or reliving hangover.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for extracting a hangover containing a natural herbal extract. BACKGROUND OF THE INVENTION < RTI ID = 0.0 >

The present invention relates to a composition for hangover decomposition containing a natural herbal medicine extract, and more particularly, to a composition for hangover decay which contains an effective ingredient of green tea seeds, a herbaceous plant extract and a lotus leaf extract as an active ingredient and has an excellent alcohol decomposing action and is easy to take.

Alcohol has been historically produced and consumed in human society for a long time, and by now, except in some religious cultures, it has been universally manufactured and consumed in most human societies. Especially in our country, it is frequent exposure to alcohol as a customary drinking culture in social life, and liver damage and health deterioration resulting from it are becoming a social problem. According to the results of the 2001 National Health and Nutrition Survey conducted by the Korea Health & Social Research Institute under the Ministry of Health and Welfare, 69.8% of adults in Korea are over 20 years old, and 30.6% , And 63.4% of drinkers. Modern people have suffered from hangovers such as thirst, fatigue, depression, memory loss, dizziness, dyspepsia, vomiting, diarrhea, and increased risk of alcoholism due to excessive alcohol consumption.

Normal alcohol metabolism is absorbed in the stomach or small intestine when alcohol is ingested, and 10% is excreted by breathing, urine, sweat, etc. 90% enters the blood vessels and metabolizes in the liver. Acetaldehyde dehydrogenase (ALDH) is decomposed into acetic acid and then transferred to muscle or fat tissue and decomposed with carbonic acid gas and water. The acetaldehyde is converted into acetaldehyde by Alcohol Dehydrogenase (ADH) do. Acetaldehyde is known to be a major cause of people feeling hangover. Acetaldehyde stimulates afferent nerve fibers in the vagus nerve and sympathetic nerves to cause symptoms such as vomiting, flushing, dizziness, Lt; / RTI > In recent research reports, it is known that besides acetaldehyde, inflammation reaction and dehydration which are induced by leukocyte stimulation by toxic alcohol such as methanol act collectively and cause hangover.

Aldehyde degrading enzymes include ALDH1 type and ALDH2 type. Of these, Asian people generally have lower ALDH2 type than Western ones, and it is known that the oxidation of acetaldehyde is slow. Accordingly, it is not uncommon to feel various hangover symptoms after drinking, and in order to reduce or prevent hangover symptoms after such drinking, there is a demand for development of a hangover resolution composition which is less effective in reducing side effects on the human body. A composition containing an extract of a herbal composition or the like is commercially available as a composition for effectively relieving a hangover due to drinking, but a composition containing a herbal medicine may cause boredom, bloating, vomiting, or the like, There is a problem that it is sold at an excessively high price and is difficult to access.

Green tea contains a large amount of antioxidants to inhibit aging, and is known as a herbal ingredient that has excellent effects on skin elasticity and moisturizing. Catechin contained in green tea is known to have anticancer effect and blood sugar suppressing effect, and it is known that it is contained in an especially rich content in green tea seeds.

Houttuyniae Herba ) is a perennial plant of Saururus chinensis, which has been widely used in folk medicine since ancient times in South East Asia, Korea and Japan. Lung abscess, pneumonia, and venereal disease.

Lotus is a perennial aquatic plant. It is known that lotus leaf contains a large amount of antioxidant and is effective for detoxification, antimicrobial action and hypotensive action.

KR 101176590 B1

It is an object of the present invention to provide a food composition which can prevent, relieve or alleviate a hangover symptom caused by drinking, by containing a green tea seed, a perch and a lotus leaf extract in a specific composition ratio.

According to the present invention, there is provided a method for producing a hanging product comprising a complex herbal medicine extract, which is prepared by mixing 20 to 30 parts by weight of a green tea seed hot water extract, 40 to 60 parts by weight of a hot water extract of Horseshoe extract and 20 to 30 parts by weight of a hot water extract of A food composition for relief is provided.

The green tea seed hydrothermal extract, Hodgson's hydrothermal extract, and Yeast Leaf hot-water extract may be prepared by adding a solvent of 10 to 20 times the weight of the extractant and extracting the mixture at 90 to 100 ° C for 2 to 4 hours.

The complex herbal extract may have a flavonoid content of 5 to 7 wt%.

In addition, it may further contain fruit concentrate, hornbill concentrate, plum concentrate, licorice concentrate, amino acid, vitamins and sweetening agent.

The fruit concentrate may be a cherry concentrate.

1 to 3 parts by weight of the complex herb extract, 0.5 to 5 parts by weight of a fruit concentrate, 1 to 5 parts by weight of a Hovenous Fruit concentrate, 0.1 to 1 part by weight of a plum concentrate, 0.01 to 0.1 part by weight of a licorice concentrate, 0.1 to 0.5 parts by weight of amino acids, 0.00001 to 0.001 parts by weight of vitamins and 10 to 15 parts by weight of sweeteners.

The food composition may also be in the form of a powder, granules, tablets, capsules or drinks.

The food composition containing the green tea seeds, the perennial herb extract and the lotus leaf extract according to the present invention exhibits excellent antioxidative activity and promotes the activity of the alcohololytic enzyme, thereby promoting the alcohol degradation metabolism and reducing the inflammatory reaction caused by the alcohol decomposition intermediate product . Through this, excellent efficacy can be exhibited in preventing, resolving or alleviating hangover symptoms.

In addition, since it does not show toxicity to tissues and is safe for human body, it can be used as a safe food composition.

In addition, it can be used as a food composition having a good acidity and containing no synthetic additives such as a synthetic flavoring agent or a preservative, and being highly health-oriented and easy to drink and highly desirable.

FIG. 1 is a graph showing the results of measuring the DPPH radical scavenging activity of the herbal medicine extract,
FIG. 2 is a graph showing the results of measuring the ABTs radical scavenging activity of the herbal medicine extract,
FIG. 3 is a graph showing the results of measuring the effect of the herbal medicine extract of the present invention on ADH activity,
FIG. 4 is a graph showing the results of measuring the effect of the herbal extract of the present invention on ALDH activity,
FIG. 5 is a graph showing the results of measuring the activity of decreasing the ethanol content in the blood after the alcohol treatment by the pretreatment of the herbal extract of the present invention,
FIG. 6 is a graph showing the results of measuring the activity of pretreatment of the herbal extract of the present invention to reduce acetaldehyde content in blood after administration of alcohol,
7 is a graph showing the taste and sensory evaluation results of the food composition containing the herbal extract of the present invention.

The present invention relates to a herbal medicine comprising a herbal extract comprising 20 to 30 parts by weight of a green tea seed hydrothermal extract, 40 to 60 parts by weight of a hydrothermal extract of Horseshoe cherry, and 20 to 30 parts by weight of a hot water extract of Leafyard as an active ingredient. ≪ / RTI >

Hereinafter, the present invention will be described in more detail.

The composition of the present invention contains an extract of a complex herbal medicine prepared by mixing green tea seeds, Rhizoctonia sp.

In the present invention, the separation of the green tea seeds, the oriental herb and the lotus leaf extract contained in the herbal medicine extracts is carried out using a hot water extraction method. Specifically, water in an amount of 10 to 20 times the weight of the extracted raw material is added to each component contained in the herbal medicine extract, and the extract is separated by hot water extraction at 90 to 100 ° C for 2 to 4 hours . When the separation of the green tea seeds, the oriental herb and the lotus leaf extract contained in the herbal medicine extract according to the present invention is carried out under the above-mentioned solvent addition amount, temperature and time condition, the extraction of the extract is optimized with high concentration and high efficiency, Can be increased. In addition, by maximizing the content of the active ingredient contained in the extract of each ingredient, the efficacy of the herbal extract of the present invention can be increased. If the content of the solvent added to each component is less than 10 times the weight of the extracted raw material for hot water extraction, the extraction content of the active ingredient, phenol compound or flavonoid, may be reduced. If the content is more than 20 times the extraction amount, The concentration of the solution is too low, which may cause a long time and cost for post-treatment such as drying. Preferably, for the standardization of the hot-water extract to be produced, it is preferable to add 15 to 20 times of water to each ingredient in a solvent as the weight of the extract. If the hot water extraction temperature is lower than 90 ° C, the extraction rate of the active ingredient lowers and the extraction time increases. If the hot water extraction temperature exceeds 100 ° C, the content of the solvent may be reduced by evaporation, thereby reducing the extraction amount of the active ingredient. The hot water extract containing the active ingredient at a high ratio can be prepared by extracting for 2 to 4 hours under the condition of the solvent addition amount and the extraction temperature as described above. The separated hot-water extract can be filtered, reduced in pressure, concentrated and dried according to a known method to produce a hot-water-extract of solid form. If necessary, the extraction source for each component for hot water extraction can be prepared by cutting, drying and crushing by a conventional method. The green tea seed extract prepared for preparing the herbal extract of the present invention may contain EGC in an amount of 3000 to 4000 μg / ml. The perennial herb extract may contain heparoside in an amount of 150 to 250 μg / ml. The extract of the lotus leaf can contain quercetin in an amount of 8000 to 12000 g / ml.

In the present invention, the complex herbal medicine extract comprises 20 to 30 parts by weight of green tea seed hydrothermal extract, 40 to 60 parts by weight of hydrothermal extract and 20 to 30 parts by weight of lotus leaf extract. When the herbal extract of the present invention contains each component in the above-mentioned weight ratio, it contains 7 wt% or more of a phenolic compound, which is an effective ingredient showing efficacy, and 5 wt% or more of a flavonoid, and exhibits excellent antioxidative activity and promoting activity of an alcoholase To maximize the effect of hangover resolution.

The composition containing the herbal extract of the present invention has an antioxidative activity and can reduce the symptoms of hanging induced by inflammation caused by acetaldehyde and methanol generated in the alcohol body. In addition, it is possible to reduce hangover symptoms by increasing the activity of enzymes involved in the body's degradation of alcohol. The composition containing the herbal extract of the present invention can be administered orally before or after the ingestion of alcohol to prevent, reduce and alleviate the symptoms of hangover, and also have a liver protecting effect for reducing liver damage caused by alcohol. Such hangover symptoms may include thirst after drinking, fatigue, deterioration in condition, decline in memory, dizziness, indigestion, vomiting, diarrhea and the like.

The composition comprising the herbal extract of the invention according to the present invention is prepared from a food composition. The food composition means that the complex herbal extract is added to a food material such as beverage, tea, spice, gum, confectionery, or the like, or is prepared by encapsulation, powdering, suspension or the like for oral administration. The form of the food composition is not particularly limited as long as it is a substance to be orally administered, and may be in the form of, for example, a powder, a granule, a tablet, a capsule or a drink.

If necessary, the composition containing the herbal extract of the present invention may further comprise components that are ordinarily added during the manufacture of food. But are not limited to, for example, amino acids, carbohydrates, fats, vitamins and nutrients. From the viewpoint of further improving the hangover efficacy, the composition comprising the herbal extract of the present invention may further comprise a stock solution or concentrate such as hornbug fruit, plum or the like. The concentrate can be prepared by a known method. For example, the extract can be prepared by concentrating the extract obtained by hot water extraction, sugar-picking, or stock solution from a raw material. The concentration can be carried out by vacuum low-temperature concentration, high-temperature concentration, reduced-pressure concentration or freeze-concentration method, and it is preferable that the concentration of the concentrate to be produced is concentrated so as to be 50% (v / v) And may be from 40 to 200 Bricks, but is not limited thereto. In addition, from the viewpoint of improving the preference at the time of ingestion, the composition comprising the herbal extract of the present invention may further comprise a fruit stock solution or concentrate, a licorice stock solution or concentrate, a sweetening agent and the like. Examples of the sweetening agent include, but are not limited to, honey, agave syrup, crystalline fructose, and citric acid. In order to improve the merchantability and produce a product that meets the well-being-environmentally friendly trend, it is preferable not to include a synthetic flavoring agent or preservative. A composition containing the herbal extract of the present invention can be prepared by mixing the herbal extract with the herb extract and the commonly added components. For example, 1 to 3 parts by weight of a complex herb extract, 0.5 to 5 parts by weight of a fruit concentrate, 1 to 5 parts by weight of a hornbill fruit concentrate, 0.1 to 1 part by weight of a plum concentrate, 0.01 to 1 part by weight of a licorice concentrate 0.1 to 0.5 parts by weight of amino acids, 0.00001 to 0.001 parts by weight of vitamins and 10 to 15 parts by weight of sweeteners, but the present invention is not limited thereto.

In one embodiment of the present invention, the food composition may be a drink. The drink may further contain flavors or natural carbohydrates. The natural carbohydrate may be monosaccharide (e.g., glucose, fructose, etc.); Disaccharides (e.g., maltose, sucrose, etc.); oligosaccharide; Polysaccharides (e.g., dextrin, cyclodextrin and the like); And sugar alcohols (e.g., xylitol, sorbitol, erythritol, etc.). As the flavor, natural flavoring agents (for example, tautatin, stevia extract, etc.) may be used alone or in combination.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail with reference to the following examples. It will be apparent to those skilled in the art that these embodiments are illustrative of the present invention and are not intended to limit the scope of the appended claims and that various changes and modifications may be made to the embodiments within the scope and spirit of the invention , And it is natural that such variations and modifications fall within the scope of the appended claims.

Example

≪ Preparation of hot water extract for each ingredient for preparing the herbal medicine extract >

1 kg of each of the green tea seeds, crustacea, and lotus leaves were crushed, and 20 times (v / w) of purified water was added to each sample weight. Using a Soxhelt heater (CHANGSHIN SCIENCE) For 3 hours to obtain hot water extract. Each of the hot-water extracts was filtered with a vacuum pump (Vision VS-97SM) and concentrated at 55 ° C using a rotary evaporator (Rotavapor R-210, heating bath B491, Vacuum Pump V700) FD8508) for 7 days at -50 < 0 > C to obtain a solid hot-water extract of each component.

<Analysis of Indicative Components of Hot Water Extracts by Ingredient for the Production of Combined Herb Medicine Extract>

As a part of the standardization of raw materials for the preparation of the hangover - relieving food composition, the indicator components were set and the indicator components were analyzed for each component.

EGC (Epicatechin gallete, C ₂₂ H ₁₈ O ₁₀ ) was selected as a component of the green tea seed. Methanol was added to 800 mg of green tea seed hot water extract, sonicated for 30 minutes, stabilized at room temperature, filtered through a 0.45 μm filter, diluted 10 times with diluted solution, and analyzed by liquid chromatography / ultraviolet absorption spectrophotometer And analyzed quantitatively at 280 nm, which is the maximum absorption wavelength. The HPLC conditions were as follows: an injection volume of 5 μl; column temperature of 40 ° C; 0.1% (v / v) phosphoric acid (A) and acetonitrile (B) as mobile phase. The flow rate was 1.0 ml / min, and the time-lapse mobile phase was performed under the conditions shown in Table 1 below. The diluted solution was prepared by mixing mobile phase A and B at a ratio of 2: 8.

Time (minutes) Mobile (%) A B 0
5
25
27
40 5
5
40
5
5 95
95
60
95
95

Analysis of green tea seeds hot water extracts result, standard curve is R ² value of 0.9993 was a precise calibration, the content of the EGC in the tea seed hot water extracts obtained by freeze-drying has been detected 3589.26㎍ / ㎖.

Hyperoside (C ₂₁ H ₂₀ O ₁₂ ) component was selected as a component for the analysis of the surface component of Hwasungcho. 10 g of methanol at 60% concentration was added to 1 g of hot water extract, and the solution was sonicated for 60 minutes, stabilized at room temperature, filtered through a 0.45 μm filter, and analyzed by liquid chromatography / ultraviolet absorption spectrophotometer And quantitatively analyzed at 362 nm. HPLC conditions were 2 ㎕ injection volume, column temperature 40 ℃ and mobile phase 0.1% phosphoric acid (A) and acetonitrile (B). The flow rate was 1.0 ml / min, and the time-lapse mobile phase was performed under the conditions shown in Table 2 below.

Time (minutes) Mobile (%) A B 0
10
40
42
50
50.2
60 15
15
25
100
100
15
15 85
85
75
0
0
85
85

As a result of analysis of the hydrothermal extract of Hwasungcho, the R ² value of the standard curve was precisely calibrated to 0.9998, and the content of hyperferrocide was 208.85 ㎍ / ㎖ in the hydrothermal extract of Hwasungcho obtained by lyophilization.

Quercetin (C ₁₆ H ₁₀ O ₇ H ₂ O) was selected as an ingredient for analyzing the surface component of the lotus leaf. 10 mg of pretreatment solution was added to 300 mg of leafy hot water extract, and hydrolysis was carried out in a heating water bath or block at 90 ° C for 60 minutes. After cooling at room temperature, it was diluted 1: 1 with methanol and analyzed by liquid chromatography / ultraviolet absorption spectrophotometer And quantitatively analyzed at a maximum absorption wavelength of 370 nm. The pretreatment solution was prepared by mixing 100 ml of ethanol, 40 ml of distilled water and 16 ml of hydrochloric acid. The HPLC conditions were as follows: an injection volume of 10 μl, a column temperature of 40 ° C and 0.1% phosphoric acid (A) and methanol (B) as mobile phase. The flow rate was 1.0 ml / min, and the mobile phase by time was performed under the conditions shown in Table 3 below.

Time (minutes) Mobile (%) A B 0
5
25
33
33.2
40 30
30
90
90
30
30 70
70
10
10
70
70

The results of the lotus leaf hot water extract, standard curve is R ² value of 0.9999 was a precise calibration, the content of kwosetin from lotus leaf hot water extract obtained by freeze-drying has been detected 10194.94㎍ / ㎖.

&Lt; Preparation and composition analysis of herbal medicine extracts >

The extracts of green tea seeds, Rhizoctonia spp., And Yeast leaf extracts were mixed in the proportions shown in Table 4 below to prepare a herbal extract, and phenolic compounds and flavonoid contents were measured. The content of phenolic compounds was measured by Folin-ciocalteu's colorimetric method. 1 ml of the crude herb extract dissolved in distilled water was dispensed in 1 ml of 50 ml tube, 9 ml of distilled water was added, and 1 ml of Folin-ciocalteu's reagent was added, mixed well and left at room temperature for 5 minutes. 10 ml of 7% Na ₂ CO ₃ was added and 4 ml of distilled water was added to make a total volume of 25 ml. The mixture was kept at room temperature for 90 minutes and then absorbed at 750 nm within 1 hour. Calculation was performed using standard curve formulas. The results were repeated three times or more and the results were expressed as mean ± standard deviation. The content of flavonoid was measured by Zhishen method. Add 4 ml of distilled water to 1 ml of complex herb extract dissolved in distilled water, add 0.3 ml of 5% NaNO _2, mix well, leave at room temperature for 6 minutes, add 0.3 ml of 10% AlCl _3, mix well 2 ml of M NaOH was added, 2.4 ml of distilled water was added, and the absorbance was measured at 510 nm. The total flavonoid content of the sample was calculated using a standard curved formula prepared with catechin, and the results were repeated three times or more, and the result was expressed as a mean value ± standard deviation.

Content (parts by weight) Phenolic compound
(wt%) Flavonoid
(wt%) Green tea seed extract Horsetail extract Lotus leaf extract Production Example 1 33 33 33 5.84 ± 0.09 4.61 ± 0.14 Production Example 2 20 40 40 9.49 + - 0.12 5.69 ± 0.17 Production Example 3 25 50 25 7.40 + - 0.38 6.49 0.31 Production Example 4 25 25 50 5.51 + - 0.63 4.84 ± 0.29

Table 4 shows that the content of the flavonoid of Preparation Example 3, which was prepared by mixing the green tea seeds, the perch and the lotus leaf extract at a weight ratio of 1: 2: 1, was the highest, followed by the green tea seeds, 1: 2: 2 by weight. The content of the phenolic compound was the highest in Production Example 2, and the following was Production Example 3. In the case of Production Examples 1 and 4, the phenolic compound content was less than 6 wt% and the flavonoid content was less than 5 wt%, which was lower than those of Production Examples 2 and 3.

<Measurement of radical scavenging activity of herbal medicine extract>

The radical scavenging activity was measured for Production Examples 1 to 4 in Table 4 above. The DPPH radical scavenging activity was measured by changing the method of Brand-Williams. After adding distilled water to each of the preparation examples, 0.02 ml of 0.1 mM DPPH was mixed with 0.38 ml of the solution, and the mixture was allowed to stand at room temperature for 30 minutes, and the absorbance was measured at 517 nm. The ABTs radical scavenging activity was measured by modifying the method of Pellegrini et al. First, 7.4 mM ABTs and 2.6 mM potassium persulphate are mixed and allowed to stand in a dark place for 24 hours to form cyan-colored ABTs ⁺ radicals, which are then stored in a refrigerator (stock solution). Stock solution and PBS were diluted to 70: 1, and 10 μl of each preparation dissolved in distilled water was added to 390 μl of this solution, and then absorbance was measured at 734 nm immediately. Distilled water was used as a control group and vitamin C was used as a positive control group. The results were repeated three times or more and the results were expressed as mean ± standard deviation. The DPPH radical scavenging activity was calculated using the following equation (1), and the results are shown in FIG. The ABTs radical scavenging activity was calculated using the following equation (2), and the results are shown in FIG.

1 shows that the activity of Preparation Example 3 is the highest at 61.43 ± 2.94%, that of Production Example 2 is 55.73 ± 0.33%, and that of Production Example 1 is 52.07 ± 0.70%.

2 shows that the activity of Preparation Example 3 was the highest at 63.91 ± 1.34%, that of Production Example 2 was 59.62 ± 2.22%, and that of Production Example 1 was 53.81 ± 1.60%.

<Measurement of alcohol decomposition activity of herbal medicine extract>

Alcohololytic activity of the herbal extracts was determined by measuring the activity of ADH and ALDH in vitro . The ADH activity was obtained by adding the above Preparation Example 3 dissolved in distilled water to a mixed solution of distilled water, tris-HCl buffer (pH 8.0), 20 mM NAD +, and ethanol, reacting with an S9 homogenates enzyme source, preincubating at 30 ° C for 5 minutes, The change in absorbance at 340 nm was measured and confirmed. ALDH activity was measured by adding the above Preparation Example 3 at a concentration of 150 μg / ml dissolved in distilled water, a mixture of tris-HCl buffer (pH 8.8), 20 mM NAD +, 1 M acetaldehyde and 0.33 M 2-mercaptoethanol, , Preincubation at 30 ° C for 5 minutes, and then measuring the change in absorbance at 340 nm for 5 minutes. The ADH and ALDH activities of the samples were shown as relative activity (%) relative to the control group. The results were repeated three times or more and the results were expressed as mean ± standard deviation. ADH activity was measured in FIG. 3, and ALDH activity was measured in FIG.

Referring to FIGS. 3 and 4, the herbal extracts of both herbs showed an increase in both ADH and ALDH activities related to alcohol metabolism.

<Toxicity evaluation of herbal medicine extract>

High content of the active ingredient with respect to preparation 3, the antioxidant effect of excellent composition of the present invention, in vivo Toxicity assays were performed prior to measuring the efficacy of the compounds in the present invention. Experimental animals were purchased with SPF (Specific pathogen free) 4 weeks old ICR male mice (Orient Bio Inc., Gyeonggi Province). Five to six animals were placed in a polycarbonate cage (278 x 420 x 200 mm) in a hood equipped with a constant temperature and humidity system and incubated at room temperature 22 ± 2 ° C, air flow rate 13 to 18 cm / sec, / h, air pressure difference 2 to 10 mm H ₂ O, dark cycle 12 hours; 7:00 to 19:00, and the illuminance was 150 to 300 Lux. The body weight was measured twice a week to observe the appearance on a regular basis, and the bedding was changed periodically to maintain cleanliness. Dietary supplementation of AIN-76 was sufficient and distilled water sterilized for drinking water was used. After a week of preliminary breeding, the animals were grouped so that the average weight of each group was the same, and the animals were divided into groups of 0, 250, 500, 750, 1000, 1500 and 2000 mg / kg body weight (bw) 3 was dissolved in distilled water and orally administered. Oral dosing was performed once a day for 14 days. The anesthesia was fasted for 12 hours and then anesthetized with carbon dioxide. After dissection of the anatomy, kidney, and spleen, the tissue weights were measured and the tissue weights corresponding to body weight were calculated using the following equation (3).

Serum was collected from the abdominal aorta and centrifuged at 3,000 rpm for 15 minutes and stored in a -70 ° C freezer. Serum ALT and AST were measured using a commercial kit (Asan Pharmaceutical, Seoul, Korea) to evaluate toxicity. The unit of activity is given as Karmen / ml and the results are expressed as mean ± standard deviation (mean ± SE). The differences between the groups were analyzed by ANOVA-test and Duncan's multiple range test at p <0.05. The body weight, tissue weight and serum evaluation results of the experimental animals are shown in Tables 5 to 7 below.

division Dosage by Ingredient
(mg / kg · bw / day) Number of experimental animals Initial weight (g) Final weight (g) Survival rate (%) Control group - 5 28.30 ± 0.55 33.30 ± 0.91 100 Production Example 3




250 5 28.96 + 0.44 34.14 + 0.09 100 500 5 28.40 + - 0.54 34.26 ± 1.53 100 750 5 29.30 0.41 32.98 + - 0.43 100 1000 5 28.02 + - 0.97 34.17 + - 0.36 100 1500 5 29.02 + -0.53 31.15 ± 1.05 100 2000 5 29.42 ± 0.82 31.46 ± 0.81 100

division Dosage by Ingredient
(mg / kg · bw / day) Relative tissue weight (%) liver kidney spleen Control group - 5.19 ± 0.15 1.79 + - 0.10 0.31 + 0.03 Production Example 3




250 5.21 ± 0.26 1.71 ± 0.17 0.31 + 0.04 500 5.46 ± 0.54 1.71 ± 0.15 0.34 + 0.03 750 5.09 ± 0.50 1.64 + - 0.12 0.35 + 0.06 1000 4.70 ± 0.34 1.81 0.26 0.29 + 0.04 1500 5.20 ± 0.27 1.41 ± 0.15 0.30 0.08 2000 4.83 ± 0.26 1.53 ± 0.06 0.31 + 0.04

division Dosage by Ingredient
(mg / kg · bw / day) ALT
(Karmen / ml) AST
(Karmen / ml) Control group - 24.95 ± 2.72 95.30 ± 2.77 Production Example 3




250 22.54 + - 0.87 95.69 + - 5.42 500 21.71 + - 0.48 89.74 ± 2.85 750 28.20 ± 0.76 93.79 ± 3.99 1000 23.91 + 1.98 99.06 + - 2.26 1500 23.59 + - 1.23 96.73 + - 4.39 2000 20.95 ± 2.51 90.29 + 2.93

Referring to Tables 5 to 7, it can be seen that all the experimental groups do not show any influence on body weight, tissue weight, serum ALT and AST activity.

<Estimation of hangover physiological activity of herbal extracts>

For Preparation Example 3, which has excellent efficacy, in vivo In order to determine the efficacy of the test. An 8-week-old male Sprague-Dawley rats of SPF (Specific pathogen free) were purchased from the experimental animals (Orient Bio Co., Ltd., Gyeonggi-do). The animals were housed in a polycarbonate cage (278 × 420 × 200 mm) in a hood equipped with a constant-temperature and constant-humidity system and incubated at room temperature 22 ± 2 ° C, air velocity 13 to 18 cm / sec, ventilation frequency 10 to 20 times / h , Air pressure difference 2 to 10 mm H2O, dark cycle 12 hours; 7:00 to 19:00, and the illuminance was 150 to 300 Lux. Periodically, the bedding was changed and kept clean. The diet supplied commercial pellet chow and did not have feed and water restriction during the experiment. After adjusting to the breeding environment for one week, the animals were randomly assigned to 8 animals in the same weight. Ethanol group, and the experimental group of Production Example 3, respectively.

On the day before the hangover experiment, the animals were fasted for 18 hours before the administration of the sample to prevent interference with the ethanol absorption of the gastrointestinal tract due to feed intake. In the normal control group and the ethanol group, sterilized distilled water was orally administered. In the experimental group, 200 mg / kg b.w./day Preparation Example 3 was orally administered. Twenty minutes after the administration of each sample, 25% ethanol (3 g / kg · b.w./day) was orally administered at 10 ml / kg to the ethanol group and the experimental group. Each preparation was dissolved in distilled water and administered. After oral administration of 3 g / kg · b · w · / day ethanol, blood samples were collected from the American artery at 1 hour, 3 hours, and 5 hours. Blood obtained by time was centrifuged at 3,000 rpm at 4 ° C for 10 minutes to obtain serum. At 7 hours, the animals were anesthetized with carbon dioxide, dissected and tissues were extracted.

The ethanol and acetaldehyde contents in blood were measured by using a spectrophotometer (Bio-Tek, Winooski, VT, USA) using the assay kit for measuring ethanol and acetaldehyde (Roche Co., Darmstadt, Germany) , And data of each group were presented as relative activity (%) based on the serum ethanol and acetaldehyde contents of the ethanol group measured for 1 hour. The change in the blood ethanol concentration is shown in Fig. 5, and the change in the acetaldehyde concentration is shown in Fig.

For the extracted liver tissues, when stored at -70 ° C, the cells were thawed and washed three times with D-PBS, and 0.1 M Tris-HCl buffer (pH 7.4) 10 times the tissue weight (g) was added. , And then ADH and ALDH activities were measured using an assay kit (Biovision, Mountain View, Calif., USA). The results were expressed in terms of nmol / min / ml using the equations given in the kit. Serum AST and ALT were also measured to confirm damage of hepatocytes. Since hepatocyte damage leads to changes in transport function and membrane permeability resulting in leakage of enzymes from the cells into the blood, many releases of ALT and AST into the circulatory system cause serious damage to the liver tissue during the alcohol- it means. Blood collected at the time of dissection was centrifuged at 3,000 rpm for 20 min, serum was obtained from the supernatant, and ALT and AST activities were analyzed using a commercial kit (Asan Pharmaceutical, Seoul, Korea) Was given as Karmen / ml. The relative weights of the extracted tissues are shown in Table 8. The results of measuring the activity of AST and ALT of serum are shown in Table 9, and the results of ADH and ALDH activities of liver tissues are shown in Table 10.

The results of all experiments were expressed as mean ± standard deviation (mean ± SE). The differences between the groups were analyzed by ANOVA-test and Duncan's multiple range test at p <0.05.

Experimental group
Relative tissue weight (%) liver kidney spleen Normal control group 2.80 ± 0.11 0.82 ± 0.01 0.20 ± 0.01 Ethanol group 3.01 ± 0.08 0.74 ± 0.01 0.18 ± 0.01 Experimental group 2.84 + 0.04 0.79 + - 0.01 0.19 ± 0.01

Experimental group ALT (Karmen / ml) AST (Karmen / ml) Normal control group 34.99 ± 2.39 134.13 + - 6.70 Ethanol group 30.57 ± 1.49 127.23 7.88 Experimental group 38.47 + - 2.70 135.00 + - 3.42

Experimental group ADH activity
(nmol / min / ml) ALDH activity
(nmol / min / ml) Normal control group 97.46 + 1.44 11.13 ± 1.11 Ethanol group 86.45 + - 4.36 9.44 ± 0.50 Experimental group 97.18 ± 1.43 11.55 + 1.30

5 and 6, in the group administered orally administered with the herbal extract preparation of the present invention, the ethanol concentration and the blood acetaldehyde concentration in the blood were significantly lowered in comparison with the ethanol group at 3 hours and 5 hours after the alcohol administration . The acetaldehyde concentration was decreased to a level similar to that of the normal control group after 3 hours from the administration of Preparation Example 3 in the experimental group. This shows that the composition according to the present invention is effective in preventing, reducing and eliminating acetaldehyde-induced hangover symptoms It can be confirmed that it has an excellent effect.

As shown in Tables 8 and 9, no significant difference in organ weight was observed between the experimental group administered with the acute alcohol and the ALT and AST activity of the serum dissected after 7 hours of acute alcohol administration It is possible to confirm that it does not exceed.

As shown in Table 10, the ADH and ALDH activities of the test group orally administered in Preparation Example 3 were significantly increased in comparison with the ethanol group in which the ADH and ALDH activities were decreased by acute alcohol administration .

&Lt; Preparation of granular composition containing complex herbal extracts >

The food composition comprising the herbal extract preparation example 3 of the present invention was prepared into a granular formulation easy to be orally administered according to the composition ratio shown in Table 11 below. In order to ensure safety, the herbal extracts of the herbal medicines were set to be taken at a dose of 1,000 mg / day in terms of the in vivo efficacy at a level below the usual intake level, and were designed with 500 mg / In order to hide the color of the extract in the present invention, the color of tablets coated with red ginger and gardenia blue was adjusted.

ingredient Compounding ratio (wt%) Content (mg) Production Example 3 71.43 500 Crystalline cellulose 25.582 179.08 Magnesium stearate One 7 Hydroxypropylmethylcellulose One 7 Gardenia Red 0.44 3.08 Gardenia Blue 0.011 0.08 Titanium dioxide 0.387 2.71 glycerin 0.15 1.05 system 100 700

&Lt; Preparation of beverage containing complex herbal medicine extract >

The food composition containing the herbal extract preparation example 3 of the present invention was mixed according to the composition ratios shown in Table 12 below, and was prepared in the form of a drink which can be easily administered orally. The dose was set to be 50 ml per day based on the commercially available hangover-free beverage. In order to improve the functionality, Hovenia dulcis extract was added and the synthetic flavor or preservative was excluded as a health - oriented concept. Honey and citric acid were used to adjust the sweetness 15 Brrix and acidity to pH 3.7. Fruit concentrate was added to improve flavor and palatability. All concentrates were purchased and used on the market.

ingredient Compounding ratio (wt%) Beverage Composition 1 (Cherry flavor) Beverage Composition 2 (boiled) Production Example 3 1.9 1.9 Cherry concentrate 1.0 - Concentrate - 1.0 Houttuyniae extract concentrate 5.0 5.0 Plum concentrate 0.5 0.5 Licorice concentrate 0.01 0.01 honey 6.0 6.0 Crystalline fructose 2.0 2.0 Citric acid 0.1 0.1 Taurine 0.1 0.1 Vitamin B1 hydrochloride 0.00004 0.0004 Vitamin B6 hydrochloride 0.00004 0.0004 Purified water 80.38992 80.38992 system 100 100

The beverage compositions 1 and 2 were subjected to a preference taste test to evaluate the marketability (merchantability). In - house evaluation teams who had received training on sensory test method were selected. All the samples were rinsed with pre - evaluation purified water, and then evaluated. The sensory test items were seven items including taste, preference, and aroma, and 7 - point scale was used. Statistical analysis was performed using SAS statistical program, ANOVA. The results were compared with each other and the significance was tested using Duncan's multiple range test at the 5% significance level. The results are shown in Table 13 and FIG.

division rater
gender Likelihood color Sour sweetness bitter End incense Overall
Likelihood hangover
Relief Beverage composition 1 male 4.2 ± 0.8 5.8 ± 0.8 6.2 ± 0.8 4.6 ± 1.1 4.8 ± 0.8 4.8 ± 0.8 5.8 ± 1.1 4.2 ± 1.1 female 4.4 ± 0.9 6.4 ± 0.5 6.2 ± 0.8 5.6 ± 1.1 6.0 ± 1.0 4.6 ± 1.1 6.2 ± 0.8 5.6 ± 0.9 Beverage composition 2 male 3.6 ± 0.9 3.8 ± 0.4 3.0 ± 1.0 4.8 ± 1.3 5.0 ± 1.0 3.8 ± 1.1 3.6 ± 1.1 4.4 ± 1.1 female 4.2 ± 0.4 3.2 ± 1.5 3.8 ± 1.1 4.2 ± 0.4 5.6 ± 1.1 4.6 ± 0.9 4.4 ± 0.5 4.6 ± 0.9

Referring to Table 13 and FIG. 7, it was found that beverage composition 1 containing cherry concentrate as a fruit concentrate had higher acceptability than beverage composition 2 containing a concentrate. The syrup showed the highest preference for beverage composition 1 containing cherry concentrate, and both men and women showed higher preference in beverage composition 1 in sweet taste. As a result of statistically checking, beverage composition 1 showed significantly higher preference than beverage composition 2 in both sour and sweet taste. In general preference, women who ingested beverage composition 1 had the highest score at 6.2, followed by men at 5.8. This tendency is similar to the preference of Sanseong and Dumyu, and it is understood that the taste of overall taste has an effect on mountains and sugar.

Claims (7)

20 to 30 parts by weight of a green tea seed hydrothermal extract, 40 to 60 parts by weight of a hydrothermal extract of Horseshoe cherry, and 20 to 30 parts by weight of a hot water extract of a leafy leaf as an active ingredient.
[Claim 2] The method according to claim 1, wherein the green tea seed hydrothermal extract, Hodgson chrysanthemum extract and hot water extract of Leaf Leaf are prepared by adding a solvent of 10 to 20 times the weight of the extractant and extracting the mixture at 90 to 100 DEG C for 2 to 4 hours &Lt; / RTI &gt;
[2] The composition according to claim 1, wherein the complex herbal extract has a flavonoid content of 5 to 7 wt%.
The food composition for hangover as claimed in claim 1, further comprising a fruit concentrate, a Hovenia dulken concentrate, a plum concentrate, a licorice concentrate, an amino acid, a vitamin and a sweetener.
The food composition according to claim 4, wherein the fruit concentrate is a cherry concentrate.
[3] The composition according to claim 1, which comprises 1 to 3 parts by weight of the herbal extract, 0.5 to 5 parts by weight of the fruit concentrate, 1 to 5 parts by weight of the fruit concentrate, 0.1 to 1 part by weight of the plum concentrate, 0.01 to 0.1 part by weight, 0.1 to 0.5 part by weight of amino acid, 0.00001 to 0.001 part by weight of vitamin and 10 to 15 parts by weight of sweetener.
The food composition for hangover as claimed in claim 1, wherein the food composition is in the form of powder, granules, tablets, capsules or drinks.

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