KR20170137436A - Composition for improving skin condition comprising herb extracts mixture - Google Patents

Abstract

The present invention relates to a composition including a composite herbal extract as effective component, capable of being safe for application to skin and having an excellent skin improvement effect. More specifically, according to the present invention, the composition includes a white sandalwood extract, a frankincense extract, a Lysimachiae Foenum-graeci Herba extract, an elecampane extract, a peach kernel extract, a dried clove bud extract, a Magnoliae Flos extract, an apricot kernel extract, and a honey extract as effective components. As such, the composition is capable of having excellent skin improvement effects including an antidiabetic effect, a skin whitening effect, a skin elasticity and wrinkle improvement effect, an antioxidizing effect, and a skin blemish improvement effect.

Description

[0001] The present invention relates to a composition for improving skin comprising a herbal extract,

The present invention relates to a composition containing as an active ingredient a complex herbal extract which is safe when applied to skin and has excellent skin improving effect.

The skin is an important body that is responsible for various physiological functions such as barrier function, body temperature control function, excretion function, which protects the human body from the external environment and prevents the internal moisture and useful components from flowing out. However, due to the following reasons, the activity of skin cells may deteriorate and the skin condition may deteriorate.

Collagen and elastin present in the dermis of the skin perform functions such as mechanical rigidity of the skin, resistance of the connective tissue, maintenance of the cohesion of the tissue, and support of the cell adhesion, and have a great influence on the constituents of the skin. It is known that such collagen is reduced by photoaging due to stress, aging, or ultraviolet irradiation, while elastin is known to be distorted in its three-dimensional structure by the degrading enzyme elastase, which is activated after exposure to ultraviolet light. As a result, the skin tissue is loosened and the elasticity is lost.

When the cell function of the epidermis is lowered, the metabolism is not smooth and the exfoliation does not occur well, and when the ultraviolet rays are received in a state where the keratin is overly accumulated, the elasticity is lowered and wrinkles are generated. If the skin is not formed on the epidermis, moisture and sebum secretion are reduced, and the skin becomes dry and wrinkles are formed.

When the skin receives ultraviolet rays, melanin is synthesized to protect the skin. The synthesized melanin is transferred to the keratinocytes of the skin through the melanoma. This keratinocyte turns over over a period of 28 days. Therefore, the melanin produced is generally lost by the keratinocyte periodically for 28 days. However, when the skin cell cycle is not properly controlled by the stress, skin aging, etc., keratinocytes do not fall out in the presentation period and the pigment such as spots, freckles, Calm occurs.

In the skin, sebum, sweat and cosmetic ingredients are decomposed into substances that are toxic to the skin by the fungus, which can cause skin irritation and inflammation. In addition, skin irritation caused by ultraviolet rays increases the production of nitrogen monoxide (NO), which is an inflammation mediator, to cause skin troubles. The production of nitrogen monoxide is mostly caused by iNOS, and it is known that iNOS is rapidly induced by stimulation such as LPS and cytokine to produce excessive NO.

A reactive oxygen species (ROS), also known as a toxic oxygen species, is a cell-generated toxic substance produced by physiological actions such as respiration and is constantly produced and extinguished, with 3-5% in normal conditions . These reactive oxygen species are free radicals such as superoxide radicals (O2-) and hydroxyl radicals (HO +), which are chemically bound to atoms or molecules that are not paired with the outermost electron orbits Highly unstable and highly reactive) or in the form of compounds with paired electrons such as hydrogen peroxide (H ₂ O ₂ ) or singlet radicals. Active oxygen species have the advantage of biologically protecting bacteria in the physiological system, but generally cause oxidation in vivo, causing harmful effects that cause disease. It has been reported that these reactive oxygen species attack biological molecules and damage cells and tissues and cause various diseases related to aging and various diseases.

Research has been continued to suppress the skin aging caused by various factors as described above, to improve whitening, wrinkles, elasticity or trouble, and to develop a substance having an antioxidative effect.

Materials having the above-mentioned effects are widely distributed in the natural world, and they have been mainly used as raw materials for foods, cosmetics, medicines and the like using materials derived from plants. However, the substances derived from such natural substances are not effective enough to be used in large quantities in order to obtain a meaningful effect, resulting in toxicity and price increase.

In order to solve the problems of such natural materials, the development of chemically synthesized materials has continued. Although they have a merit that they exert a superior effect even when used in a small amount compared to a natural substance, their use is limited due to a fatal problem that can cause large and small side effects to the human body.

Accordingly, it is inevitable to develop a substance derived from a natural product and having stability, while exhibiting an excellent effect on skin improvement.

A problem to be solved by the present invention is to provide a complex herbal medicine extract which is derived from natural materials and has stability, while exhibiting excellent effects on skin, in particular whitening, wrinkle, elasticity, trouble improvement and antioxidation.

In order to solve the above-mentioned problems, the present invention provides skin whitening, wrinkle, elasticity, and the like, which comprise, as an active ingredient, a sandalwood extract, a oriental extract, a Youngran scent extract, a wood extract, a doin extract, a clove extract, , A trouble, or a composition (for example, a cosmetic composition) for antioxidation.

[extract]

Santalia Albi Lignum is the core of tree trunks of Santalum album Linne (Danalia and Santalaceae). The core of a tree trunk is a cylindrical shape with a flat rod shape and sometimes bent. The length is not constant but it is 10 ~ 20cm in diameter. Outer surface is light yellow to yellowish brown with vertical pattern, vagina is dense and firm and not well broken. The cut side has a sharp oil mark, a ring of neck color, and a straight line extending vertically. The quality is robust and is not easy to cut. A clear scent is produced, and when it is burned, the fragrance becomes stronger. The distinctive smell is thick and the taste is spicy.

Frankincense (乳香, Olibanum, Frankincense) is frankincense tree Boswellia carterii It is a resin obtained by scarring the stem of Birdwood or other relative plants (Olive and Burseraceae). Mildness is mild, poisonous, a little spicy. The efficacy is umbilical (瘀血) or blood circulation disorder caused by limb pain (肢 痛 痛) has excellent analgesic effect.

Lysimachiae Foenum-graeci Herb is the outpipe of Lysimachia foenum - graeci Hance (Primulaceae). The outcrops are cylindrical, 40 ~ 60cm long, with vertical pattern and 3 ~ 5 lines of sharp-winged tissue attached to the ridge. Leaves are wide egg-shaped, shifted, 4 ~ 6cm in diameter, pointed with apical ends, with both sides of the lower part of the leaf extending downward along the petiole, wing-shaped, and green on the outside. Spherical capsules bloom from the axil of leaves, but fruit sacks are relatively thin and fragile and 2 to 3 cm long. It has a strong scent and taste a little sweet and bitter. Toothache, diarrhea, and oil well.

Aucklandiae Radix Aucklandia lappa Decne. (Chrysanthemum and Compositae) roots to remove the rough skin. Some features are circumferential and some are slightly curved, and those with muscular tofu have concave stem marks on the upper side and sometimes vertical cracks. Outer surface is yellowish brown or grayish brown. There are rough vertical wrinkles and wrinkles of fine mesh wrinkles and residues of side roots, and some of them are removed. The vagina is firm, faithful and difficult to break. The cross section is yellowish brown or dark brown. Pharmacological action of the bronchial and intestinal seizures released, blood pressure lowering, antimicrobial action has been reported.

Doen (桃仁) is a peach tree Prunus Persica Batsch or mountain copy Prunus davidiana Franchet (Rosaceae Rosaceae) is a ripe seed. Appearance is flat, oval shape with uneven left and right sides, one with a pointed end and the other with a rounded point. Seed shells are reddish brown or light brown, and there are epidermal cells of stone cells that are easy to fall on the surface and seem to have been sprayed with powder. Many vascular anomalies are distributed in the seed bark from the junction, and the part is depressed to form vertical wrinkles. If you put it in hot water and soften it, the translucent thin coat of seed coat and white easily falls from white cotyledon. Pharmacological actions have been reported such as vasodilatory action, increase in blood flow, stimulation of uterine contraction and uterine hemostasis, defecation, antiinflammatory action, vasodilation, analgesic action, antiallergic action and antitumor action.

Cloves are cloves of cloves Syzygium aromaticum Merrill et Perry (Cloves and Myrtaceae) buds. Appearance is dark brown or dark red, with a flat flourish, with four calyxes with thick calyxes and four petals without blooms. The petals are almost spherical in shape. There are many stamens and one pistil in the petals. It has a strong taste and flavor and paralyzes the tongue. Pharmacological actions have been reported to promote gastric secretion, analgesic, anticonvulsant, anti-inflammatory, antioxidant, anti-thrombotic, antimicrobial, antiparasitic and hypotensive effects.

God (辛夷) is a bud of the network pornographic Magnolia biondii Pampanini, Magnolia Magnolia Denudata denudata Desrousseaux, Magnolia Magnolia kobus De Candolle, and ladybird Magnolia Magnolia sprengeri Pampanini (magnolia and Magnoliaceae). Mengchulhwa is a long-bud-shaped flower bud with a length of 12 ~ 25 mm and a diameter of 8 ~ 15 mm. The lower part is usually short and has a length of about 5 mm. Above the stem there is milky white skin. Calyx is 2 ~ 3 layers with 2 pieces per each layer,

There is a flurry. The outer surface of the calyx is covered with dense white or gray-green villous, and the face is brownish brown with no hairs. The petals are nine and brown. The outer three petals are rod-shaped, about 1/4 of the length of the inner ring, and are calyx-shaped. There are 6 petals on the inner side of 2, 3 petals on each wheel and they are arranged like a wheel. Surgery and pistil are arranged in several spiral shapes. This medicine is lightweight and poor in quality. There is a direction, the taste is spicy, cool and a bit bitter. It is a bud of 2 ~ 4 cm in length and 1 ~ 2 cm in diameter. The stem of the lower part is rough and the skin of the skin is reddish brown. The outer surface of the calyx is covered with pale yellow or pale yellow-green villus, and some of the calyces attached to the calyx of the outermost layer are removed, and they are dark brown. The petals are 10 ~ 12 (15) and there is no distinct difference in the inner and outer rings. Magnolia is a flower bud, having a fuller-like egg shape ~ fusiform shape with a slightly pointed hairline, 1 ~ 4 cm in length and 7 ~ 20 mm in the middle diameter. Outer surface is a soft, glowing 5 mm thick hairs of yellowish white to greenish brown, and dark brownish, roughly scaly scales on the bottom inside. This medicine has three bracts, and when cut vertically, it has nine epiphytes, several light tan operations and a brown pistil, and it is easy to break. There is a distinctive scent, and the taste is spicy, cool and slightly bitter. Pharmacological actions such as convergence, capillary dilation, antiinflammatory, hypotensive, analgesic, sedative, skin fungi and staphylococcal inhibition have been reported.

Armeniacae Semen (杏仁) is an apricot treePrunus armeniaca I have linne. ansu Maximowicz,Prunus mandshurica I have Koehne. glabra Nakai, Siberian apricotPrunus sibirica Linne or Armenian apricotsPrunus armeniaca It is a ripe seed of Linne (Rosaceae Rosaceae). Remove the sputum, stop coughing and asthma, and moisten the intestines.

Honey is a saccharide secreted by bees (Apis mellifera). Honey has honey (natural honey) and molasses (artificial honey). Honey is divided into native honey and bee honey depending on the type of honey bee. Depending on the flowers, they are called acacia honey, sour honey, oil honey, chestnut honey, buckwheat honey, etc., and the color and taste are different according to the kinds of flowers.

The present inventors have found that all of the above extracts have a biological activity beneficial to the skin, and that the extracts of two or more of the above extracts, preferably extracts of sandalwood extract, oriental extract, Younglung aroma extract, mulberry extract, doenan extract, clove extract, And the combined extract of honey extracts have synergistic biological activity.

In the present invention, the term "extract" is intended to mean an extract obtained by the above-mentioned extraction treatment, a diluted or concentrated liquid of the extracted liquid, a dried material obtained by drying the extracted liquid, a controlled preparation or a purified product of the extracted liquid, And extracts of all formulations which can be formed using extracts.

Each of the above-mentioned extracts describes each plant and may include leaves, stems, bark, roots, flowers or flowers, fruit, seeds, sap, and whole plants in addition to the specified parts.

In order to prepare the above extract, one of ordinary skill in the art can use any suitable method known in the art. For example, a solvent extraction method can be used. The entire plant or any portion thereof may be ground (e.g., a blender) and then the extraction solvent may be treated to obtain a solvent extract. It may be subjected to a drying process (for example, drying at 40 to 70 DEG C for 15 to 50 hours) before pulverization and then pulverization. In addition, the solvent extract may be prepared in powder form by an additional process such as vacuum distillation and freeze-drying or spray-drying.

The kind of the extraction solvent used is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or mixtures thereof. Water and lower alcohols may be used alone or in combination of two or more. The solvent extract may be prepared by extracting the extract at least one time using the solvent, and the dry extract obtained by vacuum distillation or spray drying the solvent extract may be prepared.

The amount of the extraction solvent may vary depending on the kind of the extraction solvent used, but may be, for example, 1 to 20 times, or 5 to 20 times the dry weight of the target plant.

In addition, various extraction processes known in the art such as, for example, maceration, infusion, percolation, digestion, decoction, hot continuous extraction, aqueous- (For example, hydrofluoro-carbon solvent), etc.), which may be used alone or in combination with one another, may be used, for example, May be carried out by using two or more methods in combination.

[Composition]

The composition according to the present invention contains as an active ingredient an extract of sandalwood extract, oriental extract, Youngryung fragrant extract, moss extract, doen extract, clove extract, ginseng extract, goat extract and honey extract.

In the present invention, the term "included as an active ingredient" means that the extract is added to the skin composition of the present invention to such an extent that it can exhibit a skin improving effect, and various components are added as a sub ingredient And it is possible to formulate in various forms.

Each plant extract may be included in the composition according to the present invention in the following proportions. For example, the weight ratio of the extracts of sandalwood extract, oriental extract, Youngran scent extract, moss extract, doen extract, clove extract, ginseng extract, passerine extract and honey extract is 1: 0.01-10: 0.01-10: 0.01-10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.01 to 10: 0.01 to 10: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: : 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3.

The composition according to the present invention may contain at least about 0.0001%, 0.0002%, 0.0003%, 0.0004%, 0.0005%, 0.0006%, 0.0007%, 0.0008%, 0.0009%, 0.0010% 0.0021%, 0.002%, 0.002%, 0.002%, 0.0024%, 0.0025%, 0.002%, 0.002% , 0.0028%, 0.0029%, 0.0030%, 0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0037%, 0.0038%, 0.0039%, 0.0040%, 0.0041%, 0.0042% %, 0.0045%, 0.0046%, 0.0047%, 0.0048%, 0.0049%, 0.0050%, 0.0051%, 0.0052%, 0.0053%, 0.0054%, 0.0055%, 0.0056%, 0.0057%, 0.0058%, 0.0059% 0.0071%, 0.007%, 0.007%, 0.007%, 0.007%, 0.005%, 0.006%, 0.007% , 0.0078%, 0.0079%, 0.0080%, 0.0081%, 0.0082%, 0.0083%, 0.0084%, 0.0085%, 0.0086%, 0.0087%, 0.0088%, 0.0089%, 0.0090%, 0.0091%, 0.0092% %, 0.0095%, 0.0096%, 0.0097%, 0.0 0.025%, 0.0350%, 0.0375%, 0.0400%, 0.0425%, 0.0450%, 0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.005%, 0.08%, 0.0099%, 0.0100%, 0.0200%, 0.0250%, 0.0275%, 0.0300% , 0.075%, 0.0600%, 0.0625%, 0.0650%, 0.0675%, 0.0700%, 0.0725%, 0.0750%, 0.0775%, 0.0800%, 0.0825%, 0.0850%, 0.0875%, 0.0900%, 0.0925%, 0.0950% %, 0.1000, 0.1250, 0.1500, 0.1750, 0.2000, 0.2250, 0.2500, 0.2750, 0.3000, 0.3250, 0.3500, 0.3750, 0.4000, 0.4250, 0.4500, 0.4750, 0.5000, 0.5250, 0.550, 0.5750, 0.6000, 0.6250, 0.6500, 0.6750, 0.7000, 0.7250, 0.7500, 0.7750, 0.8000, 0.8250, 0.8500, 0.8750, , 0.9200%, 0.9500%, 0.9750%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2% , 2.4%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7% 4.5%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5% , 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7.0%, 7.1% 8.0%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 7%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.8%, 7.9% , 8.9%, 9.0%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 10%, 11%, 12%, 13%, 14% %, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29% The composition may comprise one or more extracts of the present invention in the range of 40%, 45%, 50%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 99%

The composition according to the present invention may also contain at least about 0.0001% to 90%, 0.001% to 90%, 0.01% to 90%, 0.1% to 90%, 0.1% to 90% %, 0.1 to 80%, 0.1 to 70%, 0.1 to 60%, 0.1 to 50%, 0.1 to 40%, 0.1 to 30%, 0.1 to 20% or 0.1 to 10% . ≪ / RTI >

The% can be calculated on the basis of the total weight of the composition or the volume relative to the total volume, and the concentration can be adjusted according to the desired effect of the composition or the product into which the composition is incorporated.

The compositions of the present invention may be formulated into any type of vehicle. Examples of suitable vehicles include, but are not limited to, emulsions (e.g., oil, water, water in silicone, water in silicone, water in heavy oil, water in oil, water in heavy water, But are not limited to, a hydro-alcoholic solution), a dry basis (e.g., lipstick and powder), a gel, ointment, paste, milk, liquid, aerosol, solid form or eye jelly.

The compositions of the present invention may also be encapsulated for delivery to a target area, such as the skin. Encapsulation techniques include, for example, the use of liposomes, follicles, and / or nanoparticles (e. G., Components that are trapped, encapsulated, and / or absorbed polymeric materials that can be used as a delivery vehicle But are not limited to, the use of biodegradable and non-biodegradable colloidal particles, including, for example, nanospheres and nanocapsules.

The composition according to the present invention can be variously commercialized. For example, cosmetic compositions, food compositions, and the like.

The cosmetic composition may be prepared, for example, in the form of a general emulsified formulation and a solubilized formulation. For example, creams, essences, serums, cosmetic ointments, sprays, oil gels, gels such as lotions such as lotion, facial lotion, body lotion and the like such as flexible lotion or nutrition lotion, nutrition cream, A lotion, a cleansing lotion, a makeup remover such as a cleansing oil, a cleansing foam, a soap, a body wash and the like such as a foundation, a pack, a sunscreen, a makeup base, a liquid type, a solid type or a spray type, But may be formulated into various forms known in the art without limitation.

In addition to the extract according to the present invention, the above-mentioned cosmetic composition may further include optional ingredients commonly known as ingredients of the cosmetic composition in the art, so long as the object of the present invention is not impaired. Emulsifying agents, stabilizers, lubricants, solvents, moisturizers (e.g. emollients, humectants, film forming agents, occlusive agents, emulsifiers, water-repellant, a UV absorber (including physical and chemical absorbents such as para-aminobenzoic acid ("PABA") and corresponding (Eg, PABA derivatives, titanium dioxide, zinc oxide, etc.), essential oils, vitamins such as A, B, C, D, E and K, trace metals such as zinc, calcium and selenium, Antioxidants (such as BHT and tocopherol), chelating agents (such as disodium EDTA and tetrasodium EDTA), antioxidants (for example, antimicrobial agents) , Preservatives (such as methylparaben and propylparaben), pH adjusting agents (such as sodium hydroxide and citric acid) (E.g., aluminum starch octenyl succinate, kaolin, corn starch, oat starch, cyclodextrin, talc, and zeolites), skin bleaching and lightening agents (e.g., Hydroquinone and beta-hydroxynaphthoic acid (e.g., hydroquinone and niacinamide lactate), wetting agents such as glycerin, propylene glycol, butylene glycol, pentylene glycol, sorbitol, urea and mannitol, exfoliants (E.g., magnesium / aluminum hydroxide stearate), skin conditioning agents (e.g., aloe extracts), and the like, , Allantoin, bisabolol, ceramide, dimethicone, hyaluronic acid, and dipotassium glycyrrhizate), and thickening agents (e.g., (E.g., silicone oils and polyorganosiloxanes), and the like, which are capable of increasing viscosity, such as carboxylic acid polymers, crosslinked polyacrylate polymers, polyacrylamide polymers, polysaccharides and gums, .

The food composition may be formulated into, for example, beverages, fortified water, energy drinks, nutritional drinks, solid foods, vitamins, supplements, etc., But is not limited thereto. Such adjuvants may include vitamins, minerals, herbs or other plants, amino acids, enzymes and metabolites. Such adjuvants are suitable for oral consumption and may be administered orally.

May be provided as a kit comprising the composition according to the present invention. The composition according to the invention is contained in a container which can contain a bottle, a metal tube, a laminate tube, a plastic tube, a dispenser, a pressure vessel, a barrier container, a package, a compartment, a lipstick container, a compact container, A cosmetic pan, or other type of container, including, but not limited to, a plastic container that is injected or blow-molded into a suitable bottle, dispenser, or package to be maintained or dispersed, . The kit may include an instruction for using the kit or composition, the instruction sheet may be described on a separate sheet, or may be described on the surface of the container surface, the surface of the container wrapper. Instructions include, but are not limited to, letters, phrases, abbreviations, pictures or symbols. The instructions may include, for example, instructions on how to use, apply and maintain the kit or composition. The container can be dispensed according to a predetermined amount.

The composition according to the present invention may be provided as a topical skin composition.

In addition, a method of topically applying the composition according to the present invention to the skin can be provided.

In the present invention, the term "topical application" means applying or applying the composition on the surface of keratinous tissue, and "topical skin composition" includes compositions suitable for topical application or application on keratinocytes. Such compositions are typically dermatologically acceptable in that they do not have excessive toxicity, incompatibility, instability, allergic response, etc. when applied to the skin. The topical skin care compositions of the present invention may have a selected viscosity to avoid significant dripping or pooling after application to the skin.

[Skin improvement effect]

The composition according to the present invention has biological activity and exerts excellent effects on skin improvement.

More specifically, the composition according to the present invention has a skin whitening effect.

The skin whitening refers to inhibiting melanin formation and inhibiting melanin formation.

As a result of measuring the amount of melanin produced by treating the melanoma cells according to the present invention, the inventors of the present invention confirmed that the melanin production inhibitory effect was enhanced compared with the case of treating each extract, It was found that the effect exerted an effect corresponding to known arbutin. It was confirmed that the composition according to the present invention was excellent in the effect of inhibiting melanin formation and exhibited excellent whitening effect.

In addition, the composition according to the present invention has an effect of improving wrinkles and elasticity.

The skin wrinkles are caused by the skin of the skin and can be caused by the cause of the gene, collagen and elastin present in the skin dermis, and the external environment.

The wrinkle improvement refers to suppressing or inhibiting the generation of wrinkles, or alleviating the already generated wrinkles.

The skin elasticity refers to elastic fibers composed of elastin existing in the dermal layer and collagen fibers called collagen. The elasticity improvement refers to suppressing or inhibiting elasticity reduction or maintaining or improving elasticity .

As a result of treating the complex extract according to the present invention, the present inventors confirmed that they exhibited an enhanced collagen synthesis promoting effect and an elastase activity inhibiting effect, respectively, as compared with the case of treating each extract, And the effect of inhibiting the activity of skin elastase was shown to exert an effect corresponding to the known quercetin. The composition according to the present invention is excellent in collagen synthesis promoting effect and elastase activity inhibiting effect and shows excellent wrinkles and elasticity improving effect.

Further, the composition according to the present invention has an effect of improving the trouble.

The above-mentioned troubles are symptoms such as skin irritation and inflammation. The inflammatory reaction is characterized by pain, fever, redness, swelling and malfunction due to external stimuli. Histologically, the permeability of the arterioles, capillaries, , Complicated symptoms including enlargement accompanied by increase, excretion of plasma containing plasma protein, migration to inflammation site of leukocyte, and skin irritation reaction are characterized by erythema, itching, fever and the like.

The improvement of the trouble refers to inhibiting or inhibiting the skin irritation reaction and the inflammation reaction, or alleviating the skin irritation reaction or the inflammation reaction already in progress.

As a result of measuring the inhibitory effect on NO production by treating the compound extract according to the present invention, the present inventors confirmed that the inhibitory effect on NO production was enhanced compared to the case of treating each extract, and NO inhibitory effect was known And exhibited an effect corresponding to that of L-NMMA. It was confirmed that the composition according to the present invention exhibits excellent skin trouble improving effect through inhibition of NO production.

In addition, the composition according to the present invention has an antioxidative effect.

The antioxidant refers to inhibition of cellular oxidation by free radicals or reactive oxygen species (ROS), which are highly reactive according to oxidative stress caused by intracellular metabolism or ultraviolet rays, and free radicals Or removal of reactive oxygen species, thereby reducing damage to the cells.

As a result of measuring the free radical scavenging ability of the complex extract according to the present invention, the inventors of the present invention confirmed that they exhibited an increased free radical scavenging ability as compared with the case of treating each extract, and found that the free radical scavenging ability of vitamin C And the effect is comparable to that of the conventional method. Thus, it was confirmed that the composition according to the present invention exhibits an excellent antioxidative effect.

In addition, the composition according to the present invention has an antiherating effect.

The glycation refers to a phenomenon in which a sugar and a protein are bound to each other due to the presence of excessive sugars and the protein is destroyed. As glycosylation progresses, it affects collagen and elastin, resulting in skin elasticity deterioration and other skin damage.

As a result of measuring the antagonism effect by treating the complex extract according to the present invention, the inventors of the present invention have confirmed that they exhibit an elevated antihyperglycosylation effect as compared with the case of treating the respective extracts, and the aminoguanidine And the effect is comparable to that of the conventional method. Thus, it was confirmed that the composition according to the present invention exhibits excellent anticarcinogenic effect.

The complex extract according to the present invention is safe for the skin and has an excellent effect for improving the skin including anti-glycation effect, skin whitening effect, skin elasticity and wrinkle improving effect, antioxidative effect and skin trouble improving effect.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, the embodiments and experimental examples according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the above-described embodiments and experiments. The embodiments and experimental examples of the present invention are provided to enable those skilled in the art to more fully understand the present invention.

Example 1: Preparation of a sandalwood extract

The sandalwood was well dried and cut into fine pieces. 100 g of dry weight was placed in a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered through a filter having a pore size of 0.2 mu m to prepare a sandalwood extract.

Example 2 Preparation of Oriental Extracts

After drying well on the frankincense, 100 g of dry weight was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare an oriental extract.

≪ Example 3 > Preparation of Youngran Scent Extract

After the Youngran Scent was well dried and finely cut, 100 g of dry weight was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 탆 to prepare a Youngran Flavor Extract.

Example 4: Preparation of wood extracts

After thoroughly drying the slices, the dried weight of 100 g was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a moss extract.

Example 5: Preparation of Dane extract

After thoroughly drying and finely grinding, 100 g of dry weight was put into a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 탆 to prepare a dried extract.

<Example 6> Preparation of clove extract

The cloves were well dried and then cut into small pieces. 100 g of dry weight was placed in a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a clove extract.

&Lt; Example 7 > Preparation of Shin extract

After the shin is dried well and divided three times, 100 g of dry weight is put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a ginseng extract.

&Lt; Example 8 > Production of Candida extract

The passengers were dried well and cut into three pieces. 100 g of the dry weight was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The cold-frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a goat extract.

Example 9 Preparation of Honey Extract

100 g of honey was placed in a flask, and the mixture was heated and extracted with 1000 g of an extraction solvent (distilled water) for 30 minutes. The extract was filtered with a filter having a pore size of 0.2 mu m to prepare a honey extract.

<Example 10> Preparation of mixed extracts of sandalwood, yuangyoung, youngryungbyeol, mokhyang, doin, clove, shinyi, goin and honey

As in the above-mentioned examples, the same amounts of black algae, ylang ylang, ylang-ylang, yam, doen, clove, shinyi, Dried, and honey extracts were prepared.

&Lt; Experimental Example 1 >

In order to confirm anti-glycation efficacy, L-arginine and glucose were used to measure glycosylation-inhibiting activity.

First, 1M L-arginine and 1M glucose were dissolved by using 1M phosphate buffer (pH 7.4), and prepared by diluting the sample to 50ppm with 1M phosphate buffer solution. 1M L-arginine and 1M phosphate buffer solution were mixed at a ratio of 1: 4, and then 80 [mu] l each was added to a 96-well plate. To each sample, 100 μl of 0.01 M aminoguanidine was added to each sample to be diluted to 50 ppm and used as a positive control. These samples were mixed well and finally glucose diluted with 1M phosphate buffer solution was added to the final concentration of glucose to 0.1 M and reacted at 70 ° C for 4 hours. The degree of saccharification was measured by measuring the absorbance of the 96-well plate at 420 nm using a spectrophotometer.

Glycation group of the following formula was an experimental group in which 1M L-arginine and 1M glucose were added to induce glycation. Absorbance was measured at 420 nm by adding 1M L-arginine and sample alone without glucose to measure the absorbance of the sample itself. The saccharide inhibitory activity can be obtained by the following formula.

 [Equation 1]

('Glycation test group' in the above equation (1) means 'Absorbance of Glycation test group').

As shown in the results of Table 1 above, it can be seen that the mixed extract is superior to the aminoguanidine, which is known as an anticarious substance, in the anticarcinogenic effect.

<Experimental Example 2> Effect of inhibiting melanin formation

In order to confirm the whitening effect through inhibition of melanin formation, a culture solution of B-16 mouse melanoma cells in rat was cultured according to the method described in Lotan R. et al. (Cancer Res. 40: 3345-3350, 1980) The total amount of melanin was measured by adding an extract. In the experiment, first the toxicity of melanoma cells in rats was evaluated and the whitening evaluation was carried out at a concentration that is not toxic. DMSO was used as a negative control group, and albutin was used as a positive control group.

Specifically, the sample was added to the medium to a final concentration of 100 ppm, arbutin was added to the medium to be 100 ppm, and melanoma cells were cultured for 3 days. Cells were then trypsinized, detached from the culture, centrifuged, and then melanin was extracted. The removed cells were incubated with 1 ml of sodium hydroxide solution (1N concentration), boiled for 10 minutes to dissolve melanin, and the absorbance was measured at 400 nm using a spectrophotometer to measure the amount of melanin produced.

The amount of melanin was measured by an absorbance of 1 × 10 ⁶ cells per unit cell, and the total amount of melanin relative to the control group was calculated as the inhibition rate (%). The results are shown in Table 2 below. As shown in FIG.

As can be seen from the results of Table 2 above, the mixed extract showed excellent melanin total amount reduction effect and was found to be useful for whitening purposes.

<Experimental Example 3> Anti-inflammatory effect

In order to confirm the anti-inflammatory effect and the improvement of skin trouble, nitric oxide (NO) production inhibition experiment was performed by GRIESS method using RAW264.7 cell line (ATCC number: CRL-2278).

Specifically, RAW264.7 cells, macrophages of mice, were subcultured several times, placed in 24-well plates so as to enter 3x10 ⁵ wells into each well, and cultured for 24 hours. Subsequently, the cell culture medium containing the sample was replaced with a final concentration of 10 ppm. At this time, L-NMMA (L-NG-Monomethylarginine), which is an inhibitor of NO production, was treated together as a positive control and cultured for 30 minutes. Lipopolysaccharide (LPS) 100 μl of the supernatant was transferred to a 96-well plate, 100 μl of GRIESS solution was added thereto, and the reaction was allowed to proceed at room temperature for 10 minutes. The absorbance at 540 nm was measured to determine the NO inhibitory effect. Calculated using Equation (2) and shown in Table 3 below. Experiments were performed three times each and expressed as average values.

&Quot; (2) &quot;

NO production inhibition rate (%) = {(absorbance of negative control - absorbance of each extract) / absorbance of negative control} x 100

As can be seen from the results of Table 3, it was found that the mixed extracts exhibited excellent activity as a natural substance when compared with the representative anti-inflammatory drug L-NMMA, although the relative activity was somewhat low.

<Experimental Example 4> Promoting collagen synthesis

The sample was added to the culture medium of human - derived fibroblasts to confirm the promoting effect of type I collagen synthesis at the cellular level. The synthesized collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay Kit). In order to measure the amount of collagen synthesis, the sample was added to a fibroblast culture medium (DMEM medium) at a final concentration of 10 ppm and cultured for 48 hours. The culture broth was taken and the degree of type 1 collagen synthesis was measured at each concentration using a PICP EIA kit And measured at 450 nm using a spectrophotometer.

For the comparison of the effects, the degree of collagen synthesis was measured in the same manner for the samples in which the culture medium of the untreated fibroblasts (negative control) and vitamin C (positive control) were added to a final concentration of 52.85 / / ml. The increase rate of collagen production was calculated by the ratio of relative collagen production to the negative control, and the results are shown in Table 4 below.

As can be seen from the results in Table 4, the collagen synthesis was increased when the mixed extract was treated, and the collagen synthesis effect was comparable to that of vitamin C, which is generally known to induce collagen synthesis.

<Experimental Example 5> Elastase activity inhibitory effect

The activity inhibitory effect of Elastase, an enzyme that degrades elastin, was confirmed as follows.

Elastase used Elastase from human leukocyte cells and MeOSuc-Ala-Ala-Pro-Val-pNA as synthetic substrate for Elastase. The buffer solution used was 100 mM Tris (pH 7.5) solution. Finally, 0.2 mU was used for the ELASTASES using a buffer solution. In addition, the synthetic substrate of Elastase was diluted with buffer solution to make a final concentration of 0.5 mM by making a 100 mM solution using DMSO. At this time, the positive control group was set to contain 10 ppm of quercetin, which is known as an inhibitor of Elastinase. Ellazease inhibition candidates were added to give a final concentration of 10 ppm. The reaction was carried out in a 96-well plate and allowed to react at room temperature for 20 minutes. Absorbance was measured at 405 nm using a spectrophotometer at intervals of 1 minute, and the slope of the absorbance versus time was determined as the activity of the enzyme. Ella stasis inhibition rates were calculated as follows.

&Quot; (3) &quot;

The inhibition rate of elastase was calculated using Equation (3) and shown in Table 5 below.

As can be seen from the results in Table 5, when the mixed extract was treated, it was found that it exhibited a better inhibitory effect on elastase activity, though the effect was somewhat weaker than that of the positive control. Therefore, it was found that the mixed extract can be used for skin regeneration and wrinkle improvement.

<Experimental Example 6> Antioxidative effect

The free radical scavenging ability of the mixed extract was measured by the 1,1-diphenyl-2-picryl hydrazyl (DPPH) method (Blois, Nature 181, 1190, 1958). DPPH is a relatively stable free radical, which shows maximum absorption at 517 nm in the presence of radicals and loses its absorbance when radicals are eliminated. DPPH was purchased from Sigma, and dissolved in methyl alcohol at a concentration of 0.15 mM.

First, 100 μl of mixed extract or vitamin C, a positive control, was added to each well of a 96-well plate. 100 μl of DPPH solution was added thereto, and the mixture was allowed to stand at room temperature for 30 minutes, and the absorbance at 517 nm was measured using a microplate reader (BioTek EL-340).

IC ₅₀ represents the concentration of the extract when the absorbance of the sample is half the absorbance of the control group. The results are shown in Table 6 below. This experiment was repeated 3 times.

As shown in Table 6, the mixed extracts showed a strong free radical scavenging ability as compared with vitamin C, confirming that the antioxidative effect was excellent.

Claims (4)

A cosmetic composition for skin whitening, wrinkle, elasticity, trouble, or antioxidation, comprising as an active ingredient an extract of sandalwood extract, oriental extract, Youngran extract, moss extract, doen extract, clove extract, ginseng extract, goat extract and honey extract. The method according to claim 1, wherein the weight ratio of the extracts selected from the group consisting of sandalwood extracts, oriental extracts, Youngran scent extracts, mulberry extracts, cinnamon extracts, clove extracts, cinnamon extracts, passerine extracts and honey extracts is 1: 0.01-10: 0.01-10: 0.01-10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10. The cosmetic composition according to claim 1, wherein the extract is extracted with a solvent selected from the group consisting of water, C1 to C4 lower alcohols, and mixtures thereof. The cosmetic composition according to claim 1, wherein the cosmetic composition is antialceration, inhibition of melanin formation, promotion of collagen synthesis, inhibition of elastase activity or free radical scavenging.