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KR20160119251A - 유인원 아과 ｂ 아데노바이러스 ｓａｄｖ-28,27,-29,-32,-33, 및 -35 및 그것의 사용 - Google Patents

유인원 아과 ｂ 아데노바이러스 ｓａｄｖ-28,27,-29,-32,-33, 및 -35 및 그것의 사용 Download PDF

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KR20160119251A
KR20160119251A KR1020167026644A KR20167026644A KR20160119251A KR 20160119251 A KR20160119251 A KR 20160119251A KR 1020167026644 A KR1020167026644 A KR 1020167026644A KR 20167026644 A KR20167026644 A KR 20167026644A KR 20160119251 A KR20160119251 A KR 20160119251A
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소우미트라 로이
제임스 엠. 윌슨
뤽 에이치. 반덴베르그
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더 트러스티스 오브 더 유니버시티 오브 펜실바니아
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Abstract

재조합 벡터는 조절 서열의 제어 하에서 유인원 아데노바이러스 28, 유인원 아데노바이러스 27, 유인원 아데노바이러스 32, 유인원 아데노바이러스 33, 및/또는 유인원 아데노바이러스 35 서열 및 이종성 유전자를 포함한다. 하나 이상의 유인원 아데노바이러스 -28, -27, -32, -33, 또는 -35 유전자를 발현시키는 셀 라인이 또한 개시된다. 벡터 및 셀 라인을 사용하는 방법이 제공된다.

Description

유인원 아과 Ｂ 아데노바이러스 ＳＡＤＶ-28,27,-29,-32,-33, 및 -35 및 그것의 사용{SIMIAN SUBFAMILY B ADENOVIRUSES SADV-28,27,-29,-32,-33, AND -35 AND USES THEREOF}
CD로 제출된 자료의 참고로써 포함
출원인은 본원에서 제공되는 CD의 서열목록 자료를 참고로써 포함한다. 이 CD는 2개로 공급되며 단지 컴퓨터로 판독가능한 형태로 "서열목록"을 함유한다. 이들 디스크를 각각 "카피 1" 및 "카피 2"의 라벨을 붙인다. 이들 디스크의 파일을 "UPN-U4611 PCT sequence listing.txt"의 라벨을 붙인다.
아데노바이러스는 약 36 킬로베이스(kb)의 게놈 크기를 가지는 이중-나선 DNA 바이러스이며, 이는 다양한 표적 조직에서 고성능 유전자 전이 및 거대 이식 유전자 수용력을 달성하는 그것의 능력에 기인하여 유전자 전달 용도를 위해 널리 사용되었다. 전통적으로 아데노바이러스의 E1 유전자는 결실되고, 선택 프로모터, 관심 유전자의 cDNA 서열 및 폴리 A 시그널로 구성되는 이식 유전자 카세트로 대체되는데, 이는 복제 결함 재조합 바이러스를 초래한다.
아데노바이러스는 3개의 주요 단백질, 헥손(II), 펜톤 염기(III) 및 혹모양 섬유(IV)와 다수의 다른 부수적 단백질, VI, VIII, IX, IIIa 및 IVa2로 구성되는 다면체형의 캡시드를 가지는 특징적인 형태를 가진다[W.C. Russell, J. Gen Virol., 81 :2573-2604 (2000년 11월)]. 바이러스 게놈은 역위 말단 반복(ITR)을 가지는 5' 말단에 공유적으로 부착되는 말단 단백질을 가지는 선형의, 이중-나선 DNA이다. 바이러스 DNA는 고염기성 단백질 VII 및 소펩티드 pX(이전에 뮤로 언급됨)와 상세하게 관련된다. 다른 단백질 V는 이 DNA-단백질 복합체와 함께 패키징되고 단백질 VI를 통해 캡시드에 구조적 연결을 제공한다. 바이러스는 또한 성숙한 감염 바이러스를 만들기 위해 일부 구조적 단백질을 처리하는데 필요한 바이러스-암호화된 프로테아제를 함유한다.
분류체계는 인간, 유인원, 소, 말, 돼지, 양, 개 및 주머니쥐 아데노바이러스를 포함하는 포유류아데노바이러스 과를 위해 개발되었다. 이 분류체계는 적혈구를 교착시키기 위해 과 내의 아데노바이러스 서열의 다른 능력에 기초하여 개발되었다. 결과는 현재 아군 A, B, C, D, E 및 F로서 언급되는 6개의 아군이었다. B.N Fields et al, (Lippincott Raven Publishers, Philadelphia, 1996)에 의해 편집된 FIELD'S VIROLOGY, 6th Ed.의 T. Shenk et al, Adenovihdae : The Viruses and their Replication", Ch. 67, p. 111-2112 참조.
재조합 아데노바이러스는 숙주 세포에 이종성 분자의 전달에 대해 설명되었다. 두 침팬지 아데노바이러스의 게놈을 설명하는 미국 특허 6,083,716 참조. 유인원 아데노바이러스, C5, C6 및 C7은 백신 벡터로서 유용한 미국 특허 7,247,472호에서 설명되었다. 다른 침팬지 아데노바이러스는 아데노바이러스 백신 담체를 제조하는데 유용한 WO 2005/1071093에서 설명되었다.
당업계에서 필요로 되는 것은 표적에 분자를 효과적으로 전달하고 모집단에서 선택된 아데노바이러스 항원형에 기존 면역의 효과를 최소화하는 벡터이다.
분리된 핵산 서열 및 유인원 아데노바이러스 28(SAdV-28), 유인원 아데노바이러스 27 (SAdV-27), 유인원 아데노바이러스 29 (SAdV-29), 유인원 아데노바이러스 32 (SAdV-32), 유인원 아데노바이러스 33 (SAdV-33) 및 유인원 아데노바이러스 35 (SAdV-35)의 아미노산 서열 및 이들 서열을 함유하는 벡터가 본원에서 제공된다. 또한 본 발명의 벡터 및 세포를 사용하는 다양한 방법이 제공된다.
본원에 기술되는 방법은 본 발명의 벡터를 투여함으로써 포유동물 환자에 하나 이상의 선택된 이종성 유전자(들)을 전달하는 단계를 수반한다. 백신접종을 위해 본원에서 기술되는 조성물의 사용은 보호성 면역 반응의 유발을 위한 선택된 항원의 제시를 허용한다. SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및 SAdV-35에 기초한 벡터는 또한 시험관내 이종성 유전자 생성물을 만들기 위해 사용될 수 있다. 이러한 유전자 생성물은 그 자체가 본원에 기술되는 것과 같은 다양한 목적을 위해 유용하다.
본 발명의 이들 및 다른 구체예 및 이점은 하기에서 더욱 상세하게 기술된다.
각각 침팬지 배설물로부터 분리된 유인원 아데노바이러스 28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및 SAdV-35로부터의 신규 핵산 및 아미노산 서열이 제공된다. 또한 재조합 단백질 또는 단편 또는 다른 시약의 시험관 내 생성에서 사용을 위한 이들 벡터를 생성하기 위한 신규 아데노바이러스 벡터 및 팩키징 셀 라인이 제공된다. 더 나아가 치료적 또는 백신 목적을 위한 이종성 분자를 전달하는데 사용을 위한 조성물이 제공된다. 이러한 치료적 또는 백신 조성물은 삽입된 이종성 분자를 전달하는 아데노바이러스 벡터를 함유한다. 게다가, 신규의 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및 SAdV-35 서열은 재조합 아데노-관련 바이러스(AAV) 벡터의 생성을 위해 필요로 되는 필수적인 헬퍼 기능을 제공하는데 유용하다. 따라서, 이러한 생성 방법에서 이들 서열을 사용하는 헬퍼 구조체, 방법 및 셀 라인이 제공된다.
핵산 또는 그것의 단편을 말할 때, 용어 "실질적인 상동성" 또는 "실질적인 유사성"은, 다른 핵산(또는 그것의 상보적 가닥)과 함께 적절한 뉴클레오티드 삽입 또는 결실에 의해 최상으로 배열될 때, 배열된 서열의 적어도 약 95 내지 99%로 뉴클레오티드 서열 동일성이 있음을 나타낸다.
아미노산 또는 그것의 단편을 말할 때, 용어 "실질적인 상동성" 또는 "실질적인 유사성"은, 다른 아미노산(또는 그것의 상보적 가닥)과 함께 적절한 아미노산 삽입 또는 결실에 의해 최상으로 배열될 때, 배열된 서열의 적어도 약 95 내지 99%로 아미노산 서열 동일성이 있음을 나타낸다. 바람직하게는, 상동성은 길이에 있어서 적어도 8개의 아미노산, 또는 더 바람직하게는 적어도 15개의 아미노산인 전장 서열, 또는 그것의 단백질, 또는 그것의 단편에 있다. 적절한 단편의 예는 본원에서 기술된다.
핵산 서열에 있어서 용어 "백분율 서열 동일성" 또는 "동일한"은 최대 대응에 대해 배열될 때 동일한 두 서열의 잔기를 말한다. 한 서열과 다른 서열을 배열하는데 갭이 필요로 될 때, 스코어링의 정도는 갭에 대한 불이익 없이 더 긴 서열에 대해 계산된다. 폴리뉴클레오티드 또는 암호화된 폴리펩티드의 기능성을 보존하는 서열은 이에 의해 더욱 밀접하게 동일하다. 서열 길이 동일성 비교는 게놈의 전장(예를 들어, 약 36 kbp), 유전자, 단백질, 서브유닛, 또는 효소의 오픈리딩프레임의 전장[예를 들어, 아데노바이러스 코딩 영역을 제공하는 표]에 걸쳐 있을 수 있고, 또는 적어도 약 500 내지 5000개의 뉴클레오티드의 단편이 요망된다. 그러나, 예를 들어, 적어도 약 9개의 뉴클레오티드, 보통 적어도 약 20 내지 24개의 뉴클레오티드, 적어도 약 28 내지 32개의 뉴클레오티드, 적어도 약 36개 또는 그 이상의 뉴클레오티드를 가지는 더 작은 단편들 사이의 동일성이 또한 요망될 수 있다. 유사하게, "백분율 서열 동일성"은 단백질, 또는 그것의 단편의 전장에 걸쳐서 아미노산 서열에 대해 용이하게 결정될 수 있다. 적절하게, 단편은 길이에 있어서 적어도 8개의 아미노산이며, 약 700개 까지의 아미노산이 있을 수 있다. 적절한 단편의 예는 본원에서 기술된다.
동일성은 디폴트 세팅에서 정의되는 바와 같은 이러한 알고리즘 및 컴퓨터 프로그램을 사용하여 용이하게 결정된다. 바람직하게는, 이러한 동일성은 단백질, 효소, 서브유닛의 전장에 걸쳐, 또는 길이에 있어서 적어도 약 8개의 단편에 걸쳐서 있다. 그러나, 동일성은 더 짧은 영역에 기초할 수 있으며, 동일성 유전자 생성물이 배치되는 사용에 적합하다.
본원에서 기술되는 바와 같은, 배열은 인터넷의 웹 서버를 통해 접근가능한 "Clustal W"와 같은 다양한 일반 공중에게 또는 상업적으로 이용가능한 Multiple Sequence Alignment 프로그램을 사용하여 수행된다. 또 다르게는, 벡터 NTI® 유틸리티[InVitrogen]가 또한 사용된다. 상기 기술된 프로그램에 함유된 것들을 포함하는 뉴클레오티드 서열 동일성을 측정하는데 사용될 수 있는 당업계에 공지된 다수의 알고리즘이 있다. 다른 예에서, 폴리뉴클레오티드 서열은 Fasta, GCG Version 6.1의 프로그램을 사용하여 비교될 수 있다. Fasta는 질의와 검색 서열 사이의 최상의 중첩 영역의 배열 및 백분율 서열 동일성을 제공한다. 예를 들어, 핵산 서열 사이의 백분율 서열 동일성은 참고로써 본원에 포함되는 GCG Version 6.1에서 제공되는 바와 같은 Fasta와 그것의 디폴트 매개변수(워드 크기 6 및 스코어링 매트릭스에 대한 NOPAM 인자)를 사용하여 결정될 수 있다. 유사하게 프로그램은 아미노산 배열을 수행하기 위해 이용가능하다. 일반적으로, 당업자가 필요하다면 이들 세팅을 변경할 수 있지만, 이들 프로그램은 디폴트 세팅에서 사용된다. 또 다르게는, 당업자는 기준 알고리즘 및 프로그램에 의해 제공되는 동일성 또는 배열의 최소한의 수준을 제공하는 다른 알고리즘 또는 컴퓨터 프로그램을 이용할 수 있다.
폴리뉴클레오티드에 사용되는 "재조합"은, 폴리뉴클레오티드가 클로닝, 제한 또는 연결 단계, 및 천연에서 발견되는 폴리뉴클레오티드와 별개인 구조체를 초래하는 다른 과정의 다양한 조합의 생성물이라는 것을 의미한다. 재조합 바이러스는 재조합 폴리뉴클레오티드를 포함하는 바이러스 입자이다. 용어는 각각 본래의 폴리뉴클레오티드 구조체의 복제물 및 본래의 바이러스 구조체의 자손을 포함한다.
"이종성"은 비교되는 독립체의 나머지로부터 유전자형으로 완전한 독립체에서 유래됨을 의미한다. 예를 들어, 플라스미드에 유전공학 기술에 의해 도입된 폴리뉴클레오티드 또는 다른 종으로부터 유래된 벡터는 이종성 폴리뉴클레오티드이다. 원래의 코딩 서열로부터 제거되고 천연에서는 연결된 것으로 발견되지 않는 코딩 서열에 작동가능하게 연결된 프로모터는 이종성 프로모터이다. 바이러스 또는 바이러스 벡터의 게놈으로 클로닝된 자리-특이적 재조합 자리(바이러스의 게놈은 천연에서는 그것을 함유하지 않는다)는 이종성 재조합 자리이다. 재조합 효소에 대한 서열을 암호화하는 폴리뉴클레오티드가 재조합효소를 정상적으로 발현하지 않는 세포를 유전적으로 변경하기 위해 사용될 때, 폴리뉴클레오티드와 재조합효소는 둘 다 세포에 이종성이다.
본 명세서 및 청구항을 통해 사용되는, 용어 "포함하다" 및 그것의 변형인 "포함하는"은 다른 성분, 요소, 완전체, 단계 등에 포괄적이다. 용어 "구성된다" 또는 "구성되는"은 다른 성분, 구성요소, 정수, 단계 등에 배타적이다.
I. 유인원 아데노바이러스 서열
본 발명은 각각이 천연에서 연관된 다른 물질로부터 분리된 유인원 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및 SAdV-35의 핵산 서열 및 아미노산 서열을 제공한다. 이들 아데노바이러스 각각은 인간 아군 B로서 동일한 아군에 있는 것으로 결정되었다.
A. 핵산 서열
본원에서 제공되는 SAdV-28 핵산 서열은 SEQ 1D NO: 1의 뉴클레오티드 1 내지 35610을 포함한다. 본원에서 제공되는 SAdV-27 핵산 서열은 SEQ ID NO: 39의 뉴클레오티드 1 내지 35592를 포함한다. 본원에 제공되는 SAdV-29 핵산 서열은 SEQ ID NO: 71의 뉴클레오티드 1 내지 35646을 포함한다. SAdV-32 핵산 서열은 SEQ ID NO: 103의 뉴클레오티드 1 내지 35588을 포함한다. 본원에 제공되는 SAdV-33 핵산 서열은 SEQ ID NO: 134의 뉴클레오티드 1 내지 35694를 포함한다. 본원에 제공되는 SAdV-35 핵산 서열은 헥손 단백질의 뉴클레오티드 1 내지 35606을 포함한다.
본원에 참고로써 포함되는 서열목록을 참조. 한 구체예에서, 본 발명의 핵산 서열은 SEQ ID NO: 1, 29, 71, 103, 134, 및 166의 서열에 상보적인 가닥뿐만 아니라 하기 서열의 서열의 대응하는 RNA 및 cDNA 서열 및 그것의 상보적 가닥을 더 포함한다. 다른 구체예에서, 핵산 서열은 서열목록과 98.5% 이상 동일한, 바람직하게는 약 99% 동일한 서열을 더 포함한다. 또한 한 구체예에서, SEQ ID NO: 1, 29, 71, 103, 134, 및 166 및 그것의 상보적 가닥에서 제공된 서열의 천연 변이체 및 공학적 변형이 포함된다. 이러한 변형은, 예를 들어, 당업계에 알려진 표지, 메틸화, 및 하나 이상의 자연적으로 발생하는 뉴클레오티드의 축퇴 뉴클레오티드로의 치환을 포함한다.
[표 1]
Figure pat00001
Figure pat00002
Figure pat00003
Figure pat00004
Figure pat00005
한 구체예에서, SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및 SAdV-35의 서열의 단편, 및 그것의 상보적 가닥, 그것에 상보적인 cDNA 및 RNA가 제공된다. 적당한 단편은 길이에 있어 적어도 15개의 뉴클레오티드이며, 기능적 단편, 즉, 생물학적 관심이 있는 단편을 포함한다. 예를 들어, 기능적 단편은 요망되는 아데노바이러스 생성물을 발현시킬 수 있고 또는 재조합 바이러스 벡터의 생성에 유용할 수 있다. 이러한 단편은 유전자 서열 및 본원의 표에 열거되는 단편을 포함한다. 표는 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및 SAdV-35 서열의 전사체 영역 및 오픈리딩 프레임을 제공한다. 특정 유전자에 대해, 전사체 및 오픈리딩프레임(ORF)은 SEQ ID NO: 1, 29, 71, 103, 134, 및 166에서 존재하는 상보적인 가닥에 위치된다. 예를 들어, E2b, E4 및 E2a 참조. 암호화된 단백질의 계산된 분자량이 또한 나타난다. SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35의 E1a 오픈리딩프레임 및 E2b 오픈리딩프레임은 내부 스플라이스 자리를 함유한다는 것에 주의한다. 이들 스플라이스 자리는 상기 표에서 기록된다.
SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 아데노바이러스 핵산 서열은 치료제로서 및 다양한 벡터 시스템 및 숙주 세포의 구성에서 유용하다. 본원에서 사용되는, 벡터는 네이키드 DNA, 플라스미드, 바이러스, 코스미드 또는 에피솜을 포함하는 어떤 적당한 핵산 분자를 포함한다. 이들 서열 및 생성물은 단독으로 또는 다른 아데노바이러스 서열 또는 분획과 조합하여, 또는 다른 아데노바이러스 또는 비-아데노바이러스 서열로부터의 요소와 조합하여 사용될 수 있다. SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 서열은 안티센스 전달 벡터, 유전자 치료 벡터, 또는 백신 벡터로서 유용하다. 따라서, 추가로 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 서열을 함유하는 핵산 분자, 유전자 전달 벡터, 및 숙주 세포가 제공된다.
예를 들어, 본 발명은 본 발명의 유인원 Ad ITR 서열을 함유하는 핵산 분자를 포함한다. 다른 예에서, 본 발명은 원하는 Ad 유전자 생성물을 암호화하는 본 발명의 유인원 Ad 서열을 함유하는 핵산 분자를 제공한다. 본 발명의 서열을 사용하여 구성되는 또 다른 핵산 분자는 본원에 제공되는 정보의 관점에서 당업자에게 용이하게 명백할 것이다.
한 구체예에서, 본원에서 확인되는 유인원 Ad 유전자 영역은 세포에 이종성 분자의 전달을 위한 다양한 벡터에서 사용될 수 있다. 예를 들어, 벡터는 패키징 숙주 세포에서 바이러스 벡터를 발생시키는 목적을 위해 아데노바이러스 캡시드 단백질(또는 그것의 단편)의 발현에 대해 발생된다. 이러한 벡터는 트랜스 발현을 위해 설계될 수 있다. 또 다르게는, 이러한 벡터는 원하는 아데노바이러스 기능을 발현시키는 서열, 예를 들어, 하나 이상의 E1a, E1b, 말단 반복 서열, E2a, E2b, E4, E4ORF6 영역을 안정하게 함유하는 세포를 제공하기 위해 설계된다.
게다가, 아데노바이러스 유전자 서열 및 그것의 단편은 헬퍼-의존 바이러스(예를 들어, 필수 기능이 결핍된 아데노바이러스 벡터, 또는 아데노-관련 바이러스(AAV))의 생성에 필요한 헬퍼 기능을 제공하는데 유용하다. 이러한 생성 방법에 대해, SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및 SAdV-35 서열은 인간 Ad에 기술된 것과 유사한 방법인 그러한 방법으로 이용될 수 있다. 그러나, SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및 SAdV-35 사이의 서열, 서열과 인간 Ad의 그것들의 차이점 때문에, SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및 SAdV-35 서열의 사용은 rAAV 생성 동안 감염성 아데노바이러스 오염물질을 생성할 수 있는 인간 Ad E1 기능을 전달하는 숙주 세포, 예를 들어, 293 세포에서 헬퍼 기능을 가지는 상동 재조합의 가능성을 크게 최소화하거나 제거한다.
아데노바이러스 헬퍼 기능을 사용하는 rAAV를 생성하는 방법은 인간 아데노바이러스 항원형과 함께 문헌에서 길이로 기술되었다. 예를 들어, 미국 특허 6,258,595 및 그것에 인용된 참고문헌을 참조. 또한, 미국 특허 5,871,982; WO 99/14354; WO 99/15685; WO 99/47691 참조. 이들 방법은 또한 비-인간 영장류 AAV 항원형을 포함하는 비-인간 항원형 AAV의 생성에 사용될 수 있다. 필요한 헬퍼 기능(예를 들어, E1a, E1b, E2a 및/또는 E4ORF6)을 제공하는 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및 SAdV-35 서열은 필요한 아데노바이러스 기능을 제공하는데 특히 유용할 수 있는 한편, 어떤 다른 아데노바이러스와 재조합의 가능성을 최소화 또는 제거하는 것은 전형적으로 인간 기원의 rAAV-패키징 세포에서 존재한다. 따라서, SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 또는 SAdV-35의 선택된 유전자 또는 오픈리딩프레임은 이들 rAAV 생성 방법에 사용될 수 있다.
또 다르게는, SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35의 서열을 기초로 한 재조합 벡터는 이들 방법에 사용될 수 있다. 이러한 재조합 아데노바이러스 유인원 벡터는, 그것의 발현을 제어하는 조절 서열의 제어하에서 예를 들어, 침팬지 Ad 서열이 예를 들어, AAV 3' 및/또는 5' ITRs 및 이식 유전자로 구성되는 rAAV 발현 카세트 옆에 배치되는 하이브리드 침팬지 Ad/AAV를 포함할 수 있다. 당업자는 또 다른 유인원 아데노바이러스 벡터 및/또는 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 유전자 서열이 아데노바이러스 헬퍼에 의존하여 rAAV 및 다른 바이러스의 생성에 유용할 것임을 인식할 것이다.
또 다른 구체예에서, 핵산 분자는 숙주 세포에서 선택된 아데노바이러스 유전자 생성물의 전달 및 발현을 위해 설계되어 원하는 생리적 효과를 이룬다. 예를 들어, SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 E1a 단백질을 암호화하는 서열을 함유하는 핵산 분자는 암 치료제로서 사용을 위해 피험자에게 전달될 수 있다. 선택적으로, 이러한 분자는 지질-계 담체에서 제형화되고, 바람직하게는 암세포를 표적화한다. 이러한 제형은 다른 암 치료제(예를 들어, 시스플라틴, 탁솔 등)와 조합될 수 있다. 본원에 제공되는 아데노바이러스 서열에 대한 또 다른 사용은 당업자에게 용이하게 명백할 것이다.
게다가, 당업자는 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 서열이 치료 및 면역 분자의 시험관 내, 생체 밖 또는 생체 내 전달을 위해 다양한 바이러스 및 비-바이러스 벡터 시스템에 대한 사용에 용이하게 적용될 수 있다는 것을 용이하게 이해할 것이다. 예를 들어, SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 유인원 Ad 서열은 다양한 rAd 및 비-rAd 벡터 시스템에 이용될 수 있다. 이러한 벡터 시스템은, 예를 들어, 플라스미드, 렌티바이러스, 레트로바이러스, 수두바이러스, 우두 바이러스, 및 특히 아데노-연관 바이러스 시스템을 포함할 수 있다. 이러한 벡터 시스템의 선택은 본 발명의 제한이 아니다.
본 발명은 추가로 본 발명의 유인원 및 유인원-유래 단백질의 생성에 유용한 분자를 제공한다. 본 발명의 유인원 Ad DNA 서열을 포함하는 폴리뉴클레오티드를 전달하는 이러한 분자는 네이키드 DNA, 플라스미드, 바이러스 또는 다른 유전적 구성요소의 형태일 수 있다.
B. SAdV -28, SAdV -27, SAdV -29, SAdV -32, SAdV -33 및/또는 SAdV -35 아데노바이러스 단백질
본원에 기술되는 아데노바이러스 핵산에 의해 암호화되는 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 또는 SAdV-35 아데노바이러스의 유전자 생성물, 예컨대, 단백질, 효소 및 그것의 단편이 제공된다. 더 나아가, 다른 방법에 의해 발생되는 이들 핵산 서열에 의해 암호화된 아미노산 서열을 가지는 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 또는 SAdV-35 단백질, 효소, 및 그것의 단편이 포함된다. 이러한 단백질은 상기 표에서 확인되는 오픈리딩프레임에 의해 암호화되는 것, 표 2의 단백질(또한 서열목록에 나타냄) 및 단백질 및 폴리펩티드의 그것의 단편을 포함한다.
[표 2]
Figure pat00006
Figure pat00007
따라서, 한 양태에서, 실질적으로 순수한, 즉, 다른 바이러스 및 단백질성 단백질이 없는 독특한 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 또는 SAdV-35 단백질이 제공된다. 바람직하게는, 이들 단백질은 적어도 10% 상동성, 더 바람직하게는 60% 상동성, 및 가장 바람직하게는 95% 상동성이다.
한 구체예에서, 독특한 유인원-유래 캡시드 단백질이 제공된다. 본원에서 사용된 바와 같은, 유인원-유래 캡시드 단백질은, 제한 없이, 키메라 캡시드 단백질, 융합 단백질, 인공 캡시드 단백질, 합성 캡시드 단백질, 및 재조합 캡시드 단백질을 포함하여, 이들 단백질의 의미에 대한 제한 없이, 상기 정의한 바와 같은 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 또는 SAdV-35 캡시드 단백질 또는 그것의 단편을 함유하는 어떤 아데노바이러스 캡시드 단백질을 포함한다.
적당하게, 이들 유인원-유래 캡시드 단백질은 다른 아데노바이러스 항원형의 캡시드 영역 또는 그것의 단편, 또는 본원에서 기술되는 바와 같은 변형된 유인원 캡시드 단백질 또는 단편과 조합하여 하나 이상의 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 또는 SAdV-35 영역 또는 그것의 단편(예를 들어, 헥손, 펜톤, 섬유 또는 그것의 단편)을 함유한다. 본원에서 사용되는 바와 같은 "변형된 굴성과 연관된 캡시드 단백질의 변형"은 변경된 캡시드 단백질, 즉, 펜톤, 헥손 또는 섬유 단백질 영역, 또는 그것의 단편, 예로써, 섬유 영역의 혹(knob) 도메인, 또는 이를 암호화하는 폴리뉴클레오티드를 포함하는데, 특이성은 변경된다. 유인원-유래 캡시드는 인간 또는 비-인간 기원일 수 있는 하나 이상의 본 발명 또는 다른 Ad 항원형과 함께 구성될 수 있다. 이러한 Ad는 ATCC, 상업적 및 학업적 공급원을 포함하는 다양한 공급원으로부터 획득될 수 있고, 또는 Ad의 서열은 GenBank 또는 다른 적당한 공급원으로부터 획득될 수 있다.
SAdV-28 (SEQ ID NO:6), SAdV-27 (SEQ ID NO: 44), SAdV-29 (SEQ ID NO: 76), SAdV-32 (SEQ ID NO: 108), SAdV-33 (SEQ ID NO: 139) 및 SAdV-35 (SEQ ID NO: 171)의 펜톤 단백질의 아미노산 서열이 제공된다. 적절하게는, 이들 펜톤 단백질, 또는 그것의 독특한 단편은 다양한 목적을 위해 이용될 수 있다. 적절한 단편의 예는 각각 상기 제공된 아미노산 넘버링 및 각각 SEQ ID NO:6, 44, 76, 108, 139, 및 171에 기초한 약 50, 100, 150, 또는 200개의 아미노산의 N-말단 및/또는 C-말단의 절단(truncation)을 가지는 펜톤을 포함한다. 다른 적당한 단편은 더 짧은 내부의, C-말단의 또는 N-말단의 단편을 포함한다. 추가로, 펜톤 단백질은 당업자에게 공지된 다양한 목적을 위해 변형될 수 있다.
또한 SAdV-28 [SEQ ID NO: 11], SAdV-27 (SEQ ID NO: 49), SAdV-29 (SEQ ID NO: 81), SAdV-32 (SEQ ID NO: 113), SAdV-33 (SEQ ID NO: 144) 및 SAdV-35 (SEQ ID NO: 176)의 헥손 단백질의 아미노산 서열이 제공된다. 적절하게는, 이들 헥손 단백질, 또는 그것의 독특한 단편은 다양한 목적을 위해 이용될 수 있다. 적절한 단편의 예는 상기 제공된 아미노산 넘버링 및 각각 SEQ ID NO: 11, 49, 81, 113, 144 또는 176에 기초한 약 50, 100, 150, 200, 300, 400, 또는 500개의 아미노산의 N-말단 및/또는 C-말단의 절단을 가지는 헥손을 포함한다. 다른 적당한 단편은 더 짧은 내부의, C-말단, 또는 N-말단의 단편을 포함한다. 예를 들어, 한 적당한 단편은 헥손 단백질, 지정된 DE1 및 FG1, 또는 그것의 고도가변 영역의 루프 영역(도메인)이다. 이러한 단편은 각각 SEQ ID NO: 11, 49, 81, 113, 144 또는 176에 대하여 유인원 헥손 단백질의 아미노산 잔기 약 125 내지 443; 약 138 내지 441, 또는 더 적은 단편을 걸치는 영역, 예로써, 약 잔기 138 내지 잔기 163; 약 170 내지 약 176; 약 195 내지 약 203; 약 233 내지 약 246; 약 253 내지 약 264; 약 287 내지 약 297; 및 약 404 내지 약 430을 걸치는 것을 포함한다. 다른 적당한 단편은 당업자에 의해 용이하게 확인될 수 있다. 추가로, 헥손 단백질은 당업계에 공지된 다양한 목적을 위해 변형될 수 있다. 헥손 단백질이 아데노바이러스의 항원형에 대한 결정요인이기 때문에, 이러한 인공 헥손 단백질은 인공 항원형을 가지는 아데노바이러스를 초래할 수 있다. 다른 인공 캡시드 단백질은 또한 침팬지 Ad 펜톤 서열 및/또는 본 발명의 섬유 서열 및/또는 그것의 단편을 사용하여 구성될 수 있다.
한 구체예에서, SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 헥손 단백질의 서열을 이용하는 변경된 헥손 단백질을 가지는 아데노바이러스가 발생될 수 있다. 헥손 단백질을 변경하는 한 적절한 방법은 참고로써 포함되는 미국 특허 5,922,315호에서 기술된다. 이 방법에서, 아데노바이러스 헥손의 적어도 하나의 루프 영역은 다른 아데노바이러스 항원형의 적어도 하나의 루프 영역으로 변경된다. 따라서, 이러한 변경된 아데노바이러스 헥손 단백질의 적어도 하나의 루프 영역은 SAdV-28 (또 다르게는, SAdV-27, SAdV-29, SAdV-32, SAdV-33 또는 SAdV-35)의 유인원 Ad 헥손 루프 영역이다. 한 구체예에서, SAdV-28 (또 다르게는, SAdV-27, SAdV-29, SAdV-32, SAdV-33 또는 SAdV-35) 헥손 단백질의 루프 영역은 다른 아데노바이러스 항원형으로부터 루프 영역으로써 대체된다. 다른 구체예에서, SAdV-28 (또 다르게는, SAdV-27, SAdV-29, SAdV- 32, SAdV-33 또는 SAdV-35) 헥손의 루프 영역은 다른 아데노바이러스 항원형의 루프 영역을 대체하기 위해 사용된다. 적절한 아데노바이러스 항원형은 본원에서 기술되는 바와 같은 인간과 비-인간 항원형 중으로부터 용이하게 선택될 수 있다. 적당한 항원형의 선택은 본 발명에서 제한되지 않는다. SAdV-28 (또 다르게는, SAdV- 27, SAdV-29, SAdV-32, SAdV-33 또는 SAdV-35) 헥손 단백질 서열에 대한 또 다른 사용은 당업자에게 용이하게 명백할 것이다.
SAdV-28 [SEQ ID NO:21], SAdV-27[SEQ ID NO: 59], SAdV-29 [SEQ ID NO: 91], SAdV-32 [SEQ ID NO: 123], SAdV-33 [SEQ ID NO: 154] 또는 SAdV-35[SEQ ID NO: 185]의 섬유 단백질의 아미노산 서열. 적절하게는, 이 섬유 단백질, 또는 그것의 독특한 단편은 다양한 목적을 위해 이용될 수 있다. 한 적절한 단편은 SEQ ID NO: 21, 59, 91, 123, 154 또는 185 내에 위치되는 섬유 혹이다. 다른 적절한 단편의 예는 SEQ ID NO: 21, 59, 91, 123, 154 또는 185에서 제공되는 아미노산 넘버링에 기초하여 약 50, 100, 150, 또는 200 아미노산의 N-말단의 및/또는 C-말단의 절단을 가지는 섬유를 포함한다. 또 다른 적절한 단편은 내부 단편을 포함한다. 추가로, 섬유 단백질은 당업자에게 공지된 다양한 기술을 사용하여 변형될 수 있다.
SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35의 단백질의 독특한 단편은 길이에 있어서 적어도 8개의 아미노산이다. 그러나, 다른 원하는 길이의 단편이 용이하게 이용될 수 있다. 게다가, 변형은 SAdV28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 유전자 생성물의 수율 및/또는 발현을 향상시키기 위해 도입될 수 있고, 예를 들어, SAdV28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 유전자 생성물의 모두 또는 단편이 향상을 위해 융합 파트너와 융합되는(직접 또는 링커를 통해) 융합 분자의 구성이 본원에서 제공된다. 다른 적절한 변형은, 제한 없이, 보통 절단되는 전- 또는 후-단백질을 제거하기 위해 및 성숙 단백질 또는 효소 및/또는 비밀 유전자 생성물을 제공하기 위한 코딩 영역의 돌연변이를 제공하기 위해 코딩 영역(예를 들어, 단백질 또는 효소)의 절단을 포함한다. 또 다른 변형은 당업자에게 용이하게 명백할 것이다. 더 나아가 본원에 제공된 SAdV28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 단백질과 적어도 약 99% 동일성을 가지는 단백질이 포함된다.
본원에서 기술되는 바와 같은, SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35의 아데노바이러스 캡시드 단백질을 함유하는 본 발명의 벡터는 중화벡터가 다른 Ad 항원형계 벡터 뿐만 아니라 다른 바이러스 벡터의 유효성을 감소시키는 용도에서의 사용에 특히 적합하다. rAd 벡터는 반복 유전자 치료 또는 부스팅 면역 반응(백신 타이터)을 위한 재투여에서 특히 유리하다.
특정 환경 하에서, 항체를 발생시키기 위한 하나 이상의 SAdV28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 유전자 생성물(예를 들어, 캡시드 단백질 또는 그것의 단편)을 사용하는 것이 바람직할 수 있다. 본원에서 사용되는 용어 "항체"는 에피토프에 특이적으로 결합할 수 있는 면역글로불린 분자를 말한다. 항체는, 예를 들어, 고친화도 폴리클로날 항체, 모노클로날 항체, 합성 항체, 키메라 항체, 재조합 항체 및 인간화된 항체를 포함하는 다양한 형태로 존재할 수 있다. 이러한 항체는 면역글로불린 분류 IgG, IgM, IgA, IgD 및 IgE로부터 기원한다.
이러한 항체는 당업계에 알려진 어떤 다수의 방법을 사용하여 발생될 수 있다. 적절한 항체는 잘-알려진 전통적인 기술, 예를 들어, Kohler 및 Milstein, 및 그것의 많은 공지된 변형에 의해 발생될 수 있다. 유사하게, 바람직한 고역가 항체는 이들 항원에서 개발된 모노클로날 또는 폴리클로날 항체에 대한 공지된 재조합 기술을 적용함으로써 발생될 수 있다[예를 들어, PCT 특허 출원 No. PCT/GB85/00392; 영국 특허 출원 공개 번호 GB2188638A; Amit et al., 1986 Science, 233:747-753; Queen et al., 1989 Proc . Nat'l . Acad . Sci . USA, 86: 10029-10033; PCT 특허 출원 번호 PCT/WO9007861; 및 Riechmann et al., Nature, 332:323-327 (1988); Huse et al, 1988a Science, 246: 1275-1281 참조]. 또 다르게는, 항체는 본 발명의 항원에 동물 또는 인간 항체의 상보성 결정 영역을 조작함으로써 생성될 수 있다. 예를 들어, E. Mark and Padlin, "Humanization of Monoclonal Antibodies", Chapter 4, The Handbook of Experimental Pharmacology, Vol. 113, The Pharmacology of Monoclonal Antibodies, Springer-Verlag (June, 1994); Harlow et al., 1999, Using Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY; Harlow et al., 1989, Antibodies: A Laboratory Manual, Cold Spring Harbor, New York; Houston et al., 1988, Proc . Natl. Acad . Sci USA 85:5879-5883; 및 Bird et al., 1988, Science 242:423-426 참조. 추가로 본 발명에 의해 항-유전자형 항체(Ab2) 및 항-항-유전자형 항체(Ab3)가 제공된다. 예를 들어, M. Wettendorff et al., "Modulation of anti-tumor immunity by anti-idiotypic antibodies." In Idiotypic Network and Diseases, ed. by J. Cerny and J. Hiernaux, 1990 J. Am. Soc . Microbiol ., Washington DC: pp. 203-229]. 이들 항-유전자형 및 항-항-유전자형 항체는 당업계에 공지된 기술을 사용하여 생성된다. 이들 항체는 진단적 및 임상적 방법 및 키트를 포함하는 다양한 목적을 위해 사용될 수 있다.
특정 환경 하에서, SAdV28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 유전자 생성물, 항체 또는 본 발명의 다른 구조체에 검출가능한 표지 또는 태그를 도입하는 것이 바람직할 수 있다. 본원에서 사용되는 바와 같은, 검출가능한 표지는 단독으로 또는 다른 분자와 상호작용하여, 검출가능한 신호를 제공할 수 있는 분자이다. 가장 바람직하게는, 표지는, 예를 들어, 면역 조직 화학 분석 또는 면역 형광 현미경검사에 의해 시각적으로 검출가능하다. 예를 들어, 적당한 표지는 플루오르세인 이소티오시아네이트 (FITC), 피코에리트린 (PE), 알로피코시아닌(APC), 코리포스핀-O (CPO) 또는 탠덤 염료, PE-시아닌-5 (PC5), 및 PE-텍사스 레드(ECD)를 포함한다. 모든 이들 형광 염료는 상업적으로 이용가능하고, 그것들의 사용은 당업계에 공지되어 있다. 다른 유용한 표지는 콜로이드 골드 표지를 포함한다. 또 다른 유용한 표지는 방사성 화합물 또는 원소를 포함한다. 추가적으로, 표지는 분석에서 측색 신호를 나타내기 위해 작동하는 다양한 효소 시스템을 포함하며, 예를 들어, 글루코오스 옥시다아제(기질로서 글루코오스를 사용)는 페록시다아제 및 테트라메틸 벤지딘(TMB)과 같은 수소 도너의 존재하에서 푸른색으로서 보이는 산화된 TMB를 생성하는 생성물로서 과산화물을 방출한다. 다른 예는 ATP, 글루코오스, 및 NAD+와 반응하는 글루코오스-6-포스페이트 탈수소효소와 함께 양고추냉이과산화효소 (HRP), 알칼리 포스파타아제 (AP), 및 헥소키나아제를 포함하여, 특히 340 nm 파장에서 증가된 흡광도로서 검출되는 NADH를 얻는다.
본원에서 기술되는 방법에서 이용되는 다른 표지 시스템은 다른 수단, 예를 들어, 주입된 염료가 적용가능한 분석에서 결과 복합체의 존재하에서 시각적 신호 표시를 제공하기 위한 표적 서열과 콘쥬게이트를 형성하는 효소 대신에 사용되는 착색 라텍스 마이크로입자[Bangs Laboratories, Indiana]에 의해 검출가능하다.
원하는 분자와 표지를 커플링 또는 결합하는 방법은 마찬가지로 통상적이며 당업자에게 공지되어 있다. 표지 부착의 공지된 방법이 기술된다[예를 들어, Handbook of Fluorescent probes and Research Chemicals, 6th Ed., R.P.M. Haugland, Molecular Probes, Inc., Eugene, OR, 1996; Pierce Catalog and Handbook, Life Science and Analytical Research Products, Pierce Chemical Company, Rockford, IL, 1994/1995 참조]. 따라서, 표지 및 커플링 방법의 선택은 본 발명을 제한하지 않는다.
SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35의 서열, 단백질, 및 단편은 재조합 생성물, 화학적 합성, 또는 다른 합성 수단을 포함하는 임의의 적절한 수단에 의해 생성될 수 있다. 적절한 생성 기술은 당업자에게 잘 공지되어 있다. 예를 들어, Sambrook et al, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press (Cold Spring Harbor, NY) 참조. 또 다르게는, 펩티드는 또한 잘 공지된 고체 상 펩티드 합성 방법(Merrifield, J. Am. Chem . Soc, 85:2149 (1962); Stewart and Young, Solid Phase Peptide Synthesis (Freeman, San Francisco, 1969) pp. 27-62)에 의해 합성될 수 있다. 이들 및 다른 적절한 생성 방법은 당업자의 지식 내이며, 본 발명의 범위를 제한하지 않는다.
게다가, 당업자는 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 서열이 치료 및 면역 분자의 시험관 내, 생체 밖 또는 생체 내 전달을 위한 다양한 바이러스 및 비-바이러스 벡터 시스템을 위한 사용에 용이하게 적용될 수 있다는 것을 용이하게 이해할 것이다. 예를 들어, 한 구체예에서, 유인원 Ad 캡시드 단백질 및 본원에서 기술되는 다른 유인원 아데노바이러스 단백질은 비-바이러스, 유전자의 단백질계 전달, 단백질 및 기타 바람직한 진단적, 치료적 및 면역적 분자에 대해 사용된다. 한 이러한 구체예에서, 본 발명의 단백질은 아데노바이러스에 대한 수용체와 함께 세포를 표적화하기 위한 분자에 직접 또는 간접적으로 연결된다. 바람직하게는, 헥손, 펜톤, 섬유 또는 세포 표면 수용체를 위한 리간드를 가지는 그것의 단편과 같은 캡시드 단백질이 이러한 표적을 위해 선택된다. 전달에 적당한 분자는 본원에서 기술되는 치료적 분자와 그것의 유전자 생성물 중에서 선택된다. 지질, 폴리Lys 등을 포함하는 다양한 링커가 링커로서 이용될 수 있다. 예를 들어, 유인원 펜톤 단백질은 Medina-Kauwe LK, et al, Gene Ther . 2001년 5월; 8(10):795-803 및 Medina-Kauwe LK, et al, Gene Ther . 2001년 12월; 8(23): 1753-1761에서 기술되는 것과 유사한 방법으로 유인원 펜톤 서열을 사용하여 융합 단백질의 생성에 의한 목적을 위해 용이하게 이용될 수 있다. 또 다르게는, 유인원 Ad 단백질 IX의 아미노산 서열은 미국 특허 출원 20010047081에 기술되는 바와 같은 세포 표면 수용체에 벡터를 표적화하기 위해 이용될 수 있다. 적당한 리간드는 CD40 항원, RGD-함유 또는 폴리리신-함유 서열 등을 포함한다. 예를 들어, 헥손 단백질 및/또는 섬유 단백질을 포함하는 또 다른 유인원 Ad 단백질은 이들 및 유사한 목적을 위해 사용될 수 있다.
또 다른 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 아데노바이러스 단백질은 당업자에게 용이하게 명백할 다양한 목적을 위하여 단독으로, 또는 다른 아데노바이러스 단백질과 조합하여 사용될 수 있다. 게다가, SAdV-28 아데노바이러스 단백질의 또 다른 사용은 당업자에게 용이하게 명백할 것이다.
II. 재조합 아데노바이러스 벡터
본원에 기술되는 조성물은 치료 또는 백신 목적을 위해 세포에 이종성 분자를 전달하는 벡터를 포함한다. 본원에서 사용되는, 벡터는 제한 없이, 네이키드 DNA, 파지, 트랜스포존, 코스미드, 에피솜, 플라스미드, 또는 바이러스를 포함하는 어떤 유전적 요소를 포함할 수 있다. 이러한 벡터는 SAdV28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 및 미니유전자의 유인원 아데노바이러스를 함유한다. "미니유전자" 또는 "발현 카세트"는 숙주 세포에서 유전자 생성물의 번역, 전사 및/또는 발현을 작동하는데 필요한 선택된 이종성 유전자 및 다른 조절 요소의 조합을 의미한다.
전형적으로, SAdV-유래된 아데노바이러스 벡터는 설계되어 선택된 아데노바이러스 유전자에 고유한 영역에서 미니유전자는 다른 아데노바이러스 서열을 함유하는 핵산 분자에 위치된다. 미니유전자는 원한다면, 영역의 기능을 방해하기 위해 존재하는 유전자 영역에 도입될 수 있다. 또 다르게는, 미니유전자는 부분적으로 또는 완전히 결실된 아데노바이러스 유전자의 자리에 삽입될 수 있다. 예를 들어, 미니유전자는 특히 선택될 수 있는 기능적 E1 결실 또는 기능적 E3 결실의 자리와 같은 자리에서 위치될 수 있다. 용어 "기능적으로 결실된" 또는 "기능적 결실"은 유전자 영역의 충분한 양이 예를 들어, 돌연변이 또는 변형에 의해 제거 또는 다르게는 손상되어, 유전자 영역은 유전자 발현의 기능적 생성물을 더 이상 생성할 수 없음을 의미한다. 원한다면, 전체 유전자 영역이 제거될 수도 있다. 유전자 파괴 또는 결실을 위한 다른 적절한 자리는 본 출원의 어디에서나 논의된다.
예를 들어, 재조합 바이러스의 발생에 유용한 생성 벡터에 대해, 벡터는 미니유전자 및 아데노바이러스 게놈의 5' 말단 또는 아데노바이러스 게놈의 3' 말단 중 하나, 또는 아데노바이러스 게놈의 5'과 3' 둘 다를 함유할 수 있다. 아데노바이러스 게놈의 5' 말단은 패키징 및 복제에 필요한 5' 시스-구성요소; 즉, 5' 역위 말단 반복 (ITR) 서열(복제의 기원으로서 작용) 및 본래의 5' 패키징 인핸서 도메인(E1 프로모터를 위한 패키징 선형 Ad 게놈 및 인핸서 요소에 필요한 서열을 함유)을 함유한다. 아데노바이러스 게놈의 3' 말단은 패키징 및 단백질 막화(encapsidation)에 필요한 3' 시스-구성요소(ITR을 포함)를 포함한다. 적절하게는, 재조합 아데노바이러스는 5' 및 3' 아데노바이러스 시스-구성요소를 함유하며, 미니유전자는 5' 및 3' 아데노바이러스 서열 사이에 위치된다. SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 기초 아데노바이러스 벡터는 또한 추가 아데노바이러스 서열을 함유할 수 있다.
적절하게는, 이들 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 기초 아데노바이러스 벡터는 본 발명의 아데노바이러스 게놈으로부터 유래된 하나 이상의 아데노바이러스 구성요소를 함유할 수 있다. 한 구체예에서, 벡터는 SAdV28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-로부터의 아데노바이러스 ITR 및 동일한 아데노바이러스 항원형으로부터의 추가 아데노바이러스 서열을 함유한다. 다른 구체예에서, 벡터는 ITR을 제공하는 것보다 다른 아데노바이러스 항원형으로부터 유래되는 아데노바이러스 서열을 함유한다.
본원에서 정의되는 바와 같이, 슈도타입화된(pseudotyped) 아데노바이러스는 아데노바이러스의 캡시드 단백질이 ITR을 제공하는 아데노바이러스 보다 다른 아데노바이러스로부터 오는 아데노바이러스를 말한다.
추가로, 키메라 또는 하이브리드 아데노바이러스는 당업자에게 공지된 기술을 사용하여 본원에 기술된 아데노바이러스를 사용하여 구성될 수 있다. 예를 들어, 미국 특허 US 7,291,498호 참조.
ITR의 아데노바이러스 공급원 및 벡터에 존재하는 어떤 다른 아데노바이러스 서열의 공급원은 본 발명을 제한하지 않는다. 다양한 아데노바이러스 균주가 American Type Culture Collection, Manassas, Virginia로부터 이용가능하고, 또는 다양한 상업적 및 기관의 공급원으로부터 이용가능하다. 추가로, 많은 이러한 균주의 서열은 예를 들어, PubMed 및 GenBank를 포함하는 다양한 데이터베이스로부터 이용가능하다. 다른 유인원 또는 인간 아데노바이러스로부터 제조된 상동 아데노바이러스 벡터는 공개된 문헌에서 기술된다[예를 들어, 미국 특허 5,240,846호 참조]. 다수의 아데노바이러스 종류의 DNA 서열은 타입 Ad5[GenBank 등록 번호 M73260]를 포함하여, GenBank로부터 이용가능하다. 아데노바이러스 서열은 항원형 2, 3, 4, 7, 12 및 40과 같은 어떤 공지된 아데노바이러스 항원형으로부터 얻을 수 있고, 또한 어떤 본원에서 확인되는 인간형을 포함한다. 유사하게 비-인간 동물(예를 들어, 유인원)을 감염시키는 것으로 알려진 아데노바이러스는 또한 본 발명의 벡터 구조체에서 사용될 수 있다. 예를 들어, 미국 특허 6,083,716호 참조.
바이러스 서열, 헬퍼 바이러스(필요하다면), 및 재조합 바이러스 입자, 및 다른 벡터 성분 및 본원에 기술되는 벡터의 구조체에서 사용되는 서열은 상기 기술된 바와 같이 획득된다. 본 발명의 SAdV28 유인원 아데노바이러스 서열의 DNA 서열은 벡터 및 이러한 벡터의 제조에 유용한 셀 라인을 구성하기 위해 사용된다.
서열 결실, 삽입, 및 다른 돌연변이를 포함하는 본 발명의 벡터를 형성하는 핵산 서열의 변형은 표준 분자 생물학적 기술을 사용하여 발생될 수 있고, 본 구체예의 범주 내이다.
A. "미니유전자"
이식 유전자의 선택, "미니유전자"의 클로닝 및 구성 및 바이러스 벡터에 그것의 삽입을 위해 사용되는 방법은 본원에서 제공되는 교시가 주어지는 당업계의 기술 내이다.
1. 이식 유전자
이식 유전자는 관심의 폴리펩티드, 단백질, 또는 다른 생성물을 암호화하는 이식 유전자 옆에 위치하는 벡터 서열에 이종성인 핵산 서열이다. 핵산 코딩 서열은 숙주 세포에서 이식 유전자 전사, 번역 및/또는 발현을 허용하는 방식으로 조절 성분에 작동가능하게 연결된다.
이식 유전자 서열의 조성은 결과 벡터가 위치될 곳에서 사용에 의존할 것이다. 예를 들어, 한 종류의 이식 유전자 서열은 발현이 검출가능한 신호를 생성할 때 리포터 서열을 포함한다. 이러한 리포터 서열은, 제한 없이, DNA 서열 암호화 β-락타마아제, β-갈락토시다아제(LacZ), 알칼린 포스파타아제, 티미딘 키나아제, 녹색 형광 단백질(GFP), 클로람페니콜 아세틸트랜스페라아제(CAT), 루시페라아제, 예를들어, CD2, CD4, CD8를 포함하는 막 결합 단백질, 인플루엔자 헤마그글루티닌 단백질, 및 당업계에 잘 공지된 다른 것을 그것과 관련된 고친화도 항체에서 포함하며, 또는 통상적인 수단, 및 특히 헤마그글루티닌 또는 Myc로부터 항원 태그 도메인에 적절하게 융합된 막 결합 단백질을 포함하는 융합 단백질에 의해 생성될 수 있다. 이들 코딩 서열은, 그것의 발현을 작동시키는 조절 요소와 결합될 때, 효소, 방사선 촬영, 측색, 형광 또는 다른 분광기 분석, 형광 활성화 세포 정렬 분석 및 효소면역분석(ELISA), 방사면역측정법(RIA) 및 면역 조직 화학을 포함하는 면역 분석을 포함하는 통상적인 수단에 의해 검출가능한 신호를 제공한다. 예를 들어, 마커 서열은 LacZ 유전자이며, 신호를 전달하는 벡터의 존재는 베타-갈락토시다아제 활성에 대한 분석에 의해 검출된다. 이식 유전자가 GFP 또는 루시페라아제인 경우, 신호를 전달하는 벡터는 광도계에서 색 또는 광 생성에 의해 시각적으로 측정될 수있다.
한 구체예에서, 이식 유전자는 단백질, 펩티드, RNA, 효소, 또는 촉매적 RNA와 같은 생물 및 의학에서 유용한 생성물을 암호화하는 비-마커 서열이다. 바람직한 RNA 분자는 tRNA, dsRNA, 리보솜 RNA, 촉매적 RNA, 및 안티센스 RNA를 포함한다. 유용한 RNA 서열의 한 예는 처치 동물에서 표적 핵산 서열의 발현을 끝내는 서열이다.
이식 유전자는 암 치료제 또는 백신으로서, 면역 반응의 유발, 및/또는 예방 백신 목적을 위한 예를 들어, 유전적 결함의 치료에 사용될 수 있다. 본원에서 사용되는 바와 같은, 면역 반응의 유발은 분자에서 T 세포 및/또는 체액성 면역반응을 유발하는 분자의 능력(예를 들어, 유전자 생성물)을 말한다. 본 발명은 추가로 예를 들어, 멀티-서브유닛 단백질에 의해 야기되는 질환을 고치거나 또는 완화하기 위해 다양한 이식 유전자를 사용하는 것을 포함한다. 특정 상황에서, 다른 이식 유전자는 단백질의 각 서브유닛을 암호화하고, 또는 다른 펩티드 또는 단백질을 암호화하기 위해 사용될 수 있다. 이는 단백질 서브유닛을 암호화하는 DNA의 크기가 클 때, 예를 들어, 면역글로불린, 혈소판-유래 성장인자, 또는 디스트로핀 단백질에 대해 바람직하다. 멀티-서브유닛 단백질을 생성하기 위한 세포를 위해, 세포는 각각의 다른 서브유닛을 함유하는 재조합 바이러스로 감염된다. 또 다르게는, 단백질의 다른 서브유닛은 동일한 이식 유전자에 의해 암호화될 수 있다. 이 경우에, 단일 이식 유전자는 내부 리보자임 유입 자리(IRES)에 의해 분리된 각 서브유닛에 대한 DNA와 함께, 각각의 서브유닛을 암호화하는 DNA를 포함한다. 이는 각각의 서브유닛을 암호화하는 DNA의 자리가 작을 때, 예를 들어, 서브유닛 및 IRES를 암호화하는 DNA의 전체 크기가 5 킬로베이스 미만일 때, 바람직하다. IRES에 대한 대안으로서, DNA는 번역-후 사건에서 자기-절단하는 2A 펩티드를 암호화하는 서열에 의해 분리될 수 있다. 예를 들어, ML. Donnelly, et al., J. Gen. Virol ., 78(Pt 1): 13-21 (1997년 1월); Furler, S., et al, Gene Ther ., 8(11):864-873 (2001년 6월); Klump H., et al, Gene Ther ., 8(10):811-817(2001년 5월) 참조. 이 2A 펩티드는 IRES보다 상당히 더 작으며, 공간이 제한 인자일 때 사용에 적합하도록 만든다. 그러나, 선택된 이식 유전자가 어떤 생물학적으로 활성인 생성물 또는 다른 생성물, 예를 들어, 연구에 바람직한 생성물을 암호화할 수도 있다.
적당한 이식 유전자는 당업자에 의해 용이하게 선택될 수 있다. 이식 유전자의 선택은 이 구체예를 제한하는 것으로 고려되지 않는다.
2. 조절 요소
미니유전자에 대해 상기 확인된 주요 요소에 더하여, 벡터는 또한 플라스미드 벡터로 트랜스펙팅된 또는 본 발명에 의해 생성되는 바이러스로 감염된 세포에서 그것의 전사, 번역 및/또는 발현을 허용하는 방식으로 이식 유전자에 작동가능하게 연결되는 필요한 통상적인 조절 요소를 포함한다. 본원에 사용되는 바와 같은, "작동가능하게 연결된" 서열은 관심의 유전자와 인접하는 발현 조절 서열 및 트랜스에서 또는 관심의 유전자를 조절하기 위한 거리에서 작용하는 발현 조절 서열을 둘 다 포함한다.
발현 조절 서열은 적절한 전사, 개시, 종결, 프로모터 및 인핸서 서열; 스플라이싱 및 폴리아데닐화(폴리A) 신호와 같은 효율적인 RNA 처리 신호; 세포질 mRNA를 안정화하는 서열; 번역 효율을 향상시키는 서열(즉, Kozak 일치 서열); 단백질 안정성을 향상시키는 서열; 및 필요하다면, 암호화된 생성물의 분비를 향상시키는 서열을 포함한다.
원래의, 구성의, 유도의 및/또는 조직-특이적인 프로모터를 포함하는 매우 다수의 발현 조절 서열은 당업계에 공지되어 있으며 이용될 수 있다. 구성 프로모터의 예는, 제한없이 로우스육종바이러스 (RSV) LTR 프로모터(선택적으로 RSV 인핸서와 함께), 시토메갈로 바이러스(CMV) 프로모터(선택적으로 CMV 인핸서와 함께)[예를 들어, Boshart et al, Cell, 41:521-530 (1985) 참조], SV40 프로모터, 디히드로폴레이트 환원효소 프로모터, β-액틴 프로모터, 포스포글리세롤 키나아제(PGK) 프로모터, 및 EF1α 프로모터[Invitrogen]를 포함한다.
유도성 프로모터는 유전자 발현의 조절을 허용하고 외인성으로 공급된 화합물, 온도와 같은 환경적 인자, 또는 특이적인 생리적 상태의 존재, 예를 들어, 급성 병기, 세포의 특정 분화 상태, 또는 단지 세포를 복제하는 것에 의해 조절될 수 있다. 유도성 프로모터 및 유도성 시스템은, 제한 없이, Invitrogen, Clontech 및 Ariad를 포함하는 다양한 상업적 공급원으로부터 이용가능하다. 많은 다른 시스템이 기술되었고 당업자에 의해 용이하게 선택될 수 있다. 예를 들어, 유도성 프로모터는 아연-유도성 양 메탈로티오닌(MT) 프로모터 및 덱사메타손(Dex)-유도성 마우스 유방 종양 바이러스 (MMTV) 프로모터를 포함한다. 다른 유도성 시스템은 T7 폴리머라아제 프로모터 시스템[WO 98/10088]; 엑디손 곤충 프로모터 [No et al, Proc. Natl . Acad . Sci . USA, 93:3346-3351 (1996)], 테트라사이클린-억제성 시스템[Gossen et al, Proc . Natl . Acad . Sci . USA, 89:5547-5551 (1992)], 테트라사이클린-유도성 시스템[Gossen et al, Science, 268:1766-1769 (1995), 또한 Harvey et al, Curr . Opin . Chem . Biol ., 2:512-518 (1998) 참조]을 포함한다. 다른 시스템은 카스트라디올(castradiol), 디페놀 무리슬레론(diphenol murislerone)을 사용하는 FK506 다이머, VP16 또는 p65, RU486-유도성 시스템[Wang et al, Nat. Biotech., 15:239-243 (1997) 및 Wang et al, Gene Ther ., 4:432-441 (1997)] 및 라파마이신-유도성 시스템[Magari et al, J. Clin . Invest., 100:2865-2872 (1997)]을 포함한다. 일부 유도성 프로모터의 유효성은 시간에 따라 증가한다. 이러한 경우에, 탠덤에서 다양한 억제물질을 삽입함으로써 이러한 시스템, 예를 들어, IRES에 의해 TetR에 연결된 TetR의 효율성을 향상시킬 수 있다. 또 다르게는, 원하는 기능에 대한 스크리닝 전에 적어도 3일을 기다릴 수 있다. 이 시스템의 효율성을 향상시키기 위해 공지된 수단에 의해 원하는 단백질의 발현을 향상시킬 수 있다. 예를 들어, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)를 사용한다.
다른 구체예에서, 이식 유전자에 대한 원래의 프로모터가 사용될 것이다. 본래 프로모터는 이식 유전자의 발현이 본래 발현을 모방하는 것으로 소망될 때 바람직할 수 있다. 원래 프로모터는 이식 유전자의 발현이 일시적으로 또는 발달적으로, 또는 조직-특이적 방법으로, 또는 특이적 전사 자극에 대한 반응으로 조절되어야 할 때, 사용될 수 있다. 추가 구체예에서, 인핸서 요소, 폴리아데닐화 자리 또는 Kozak 일치 서열과 같은 다른 본래 발현 조절 요소는 또한 본래 발현을 모방하도록 사용될 수 있다.
이식 유전자의 다른 구체예는 조직-특이적 프로모터에 작동가능하게 연결된 이식 유전자를 포함한다. 예를 들어, 골격근에서 발현이 소망된다면, 근육에서 활성인 프로모터가 사용되어야 한다. 이들은 골격의 β-액틴, 미오신 경사슬 2A, 디스트로핀, 근육 크레아틴 키나아제를 암호화하는 유전자로부터의 프로모터뿐만 아니라 자연적으로 발생하는 프로모터보다 더 높은 활성을 가지는 합성 근육 프로모터를 포함한다(Li et al., Nat. Biotech., 17:241-245 (1999)). 조직-특이적인 프로모터의 예는 간(알부민, Miyatake et al, J. Virol , 71 :5124-32 (1997); B형 간염바이러스 코어 프로모터, Sandig et al, Gene Ther ., 3: 1002-9 (1996); 알파-태아 단백질(AFP), Arbuthnot et al., Hum. Gene Ther ., 7: 1503-14 (1996)), 뼈 오스테오칼신(Stein et al, Mol . Biol . Rep., 24:185-96 (1997)); 뼈 시알로단백질(Chen et al, J. Bone Miner. Res., 11:654-64 (1996)), 림프구 (CD2, Hansal et al, J. Immunol, 161:1063-8 (1998); 면역글로불린 중사슬; T 세포 수용체 사슬), 뉴런-특이적 에놀라아제(NSE) 프로모터와 같은 신경세포(Andersen et al, Cell. Mol. Neurobiol, 13:503-15 (1993)), 신경미세섬유 경-사슬 유전자(Piccioli et al, Proc . Natl . Acad . Sci USA, 88:561 1-5 (1991)), 및 특히 뉴런-특이적 vgf 유전자(Piccioli et al, Neuron, 15:373-84 (1995))에 대해 알려져 있다.
선택적으로, 치료적으로 유용한 또는 면역성 생성물을 암호화하는 이식 유전자를 전달하는 벡터는 또한 선택가능한 마커를 포함할 수 있고, 또는 리포터 유전자는 특히 제네티신, 하이그로미신 또는 퓨리마이신 저항을 암호화하는 서열을 포함할 수 있다. 이러한 선택가능한 리포터 또는 마커 유전자(바람직하게는 바이러스 입자안으로 패키징되는 바이러스 게놈 밖에 위치됨)는 암피실린 저항과 같은 박테리아 세포에서 플라스미드의 존재를 표시하는데 사용될 수 있다. 벡터의 다른 성분은 복제의 기원을 포함할 수 있다. 이들 및 다른 프로모터 및 벡터 요소의 선택은 통상적이며 많은 이러한 서열이 이용가능하다[예를 들어, Sambrook et al, 및 그것에 인용된 참고문헌 참조].
이들 벡터는 당업자에게 공지된 기술과 함께, 본원에 제공된 기술 및 서열을 사용하여 발생된다. 이러한 기술은 문헌[Sambrook et al, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press, Cold Spring Harbor, NY]에서 기술되는 것과 같은 cDNA의 통상적인 클로닝 기술, 아데노바이러스 게놈의 중복 올리고뉴클레오티드 서열의 사용, 폴리머라아제 연쇄 반응, 및 원하는 뉴클레오티드 서열을 제공하는 어떤 적당한 방법을 포함한다.
III. 바이러스 벡터의 생성
한 구체예에서, 유인원 아데노바이러스 플라스미드(또는 다른 벡터)는 아데노바이러스 벡터를 만드는데 사용된다. 한 구체예에서, 아데노바이러스 벡터는 복제-결함의 아데노바이러스 입자이다. 한 구체예에서, 아데노바이러스 입자는 E1a 및/또는 E1b 유전자에서 결실에 의한 복제-결함이 제공된다. 또 다르게는, 아데노바이러스는, 선택적으로 E1a 및/또는 E1b 유전자를 보유하는 동안 다른 수단에 의한 복제-결함이 제공된다. 아데노바이러스 벡터는 또한 아데노바이러스 게놈에서 다른 돌연변이, 예를 들어, 다른 유전자에서 온도-민감 돌연변이 또는 결실을 함유할 수 있다. 다른 구체예에서, 아데노바이러스 벡터에서 무결함 E1a 및/또는 E1b 영역을 보유하는 것이 바람직하다. 이러한 무결함 E1 영역은 아데노바이러스 게놈에서 그것의 본래 위치에서 위치될 수도 있고 또는 본래 아데노바이러스 게놈에서 결실 자리(예를 들어, E3 영역)에 위치될 수도 있다.
인간(또는 다른 포유동물) 세포에 유전자의 전달을 위해 유용한 유인원 아데노바이러스 벡터의 구성에서, 아데노바이러스 핵산 서열의 범위는 벡터에서 사용될 수 있다. 예를 들어, 모든 또는 일부의 아데노바이러스 지연 초기 유전자 E3은 재조합 바이러스의 부분을 형성하는 유인원 아데노바이러스 서열로부터 제거될 수 있다. 유인원 E3의 기능은 재조합 바이러스 입자의 기능 및 생성에 무관한 것으로 믿어진다. 유인원 아데노바이러스 벡터는 또한 E4 유전자의 적어도 ORF6 영역의 결실을 가지도록, 더 바람직하게는 이 영역, 전체 E4 영역 기능의 불필요한 중복 때문에 구성될 수 있다. 본 발명의 또 다른 벡터는 지연된 초기 유전자 E2a에서 결실을 함유한다. 결실은 또한 유인원 아데노바이러스 게놈의 L5를 통해 어떤 말기 유전자 L1에서 만들어질 수 있다. 유사하게, 중간 유전자 IX 및 IVa2의 결실은 일부 목적에 유용할 수 있다. 다른 결실은 다른 구조적 또는 비-구조적 아데노바이러스 유전자에서 만들어질 수 있다. 상기 논의된 결실은 개개로 사용될 수 있고, 즉, 본원에 기술되는 바와 같은 사용을 위한 아데노바이러스 서열은 단지 단일 영역에서 결실을 함유할 수 있다. 또 다르게는, 전체 유전자 또는 그것의 생물학적 활성을 파괴하는데 효과적인 그것의 부분의 결실은 어떤 조합으로 사용될 수 있다. 예를 들어, 한 예시적인 벡터에서, 아데노바이러스 서열은 E1 유전자 및 E4 유전자, 또는 E1, E2a 및 E3 유전자, 또는 E1 및 E3 유전자, 또는 E3 등의 결실과 함께 또는 결실 없이, E1, E2a 및 E4 유전자의 결실을 가질 수 있다. 상기 논의한 바와 같이, 이러한 결실은 원하는 결과를 이루기 위해 온도-민감 돌연변이와 같은 다른 돌연변이와 조합하여 사용될 수 있다.
어떤 필수 아데노바이러스 서열을 결핍하는 아데노바이러스 벡터(예를 들어, E1a, E1b, E2a, E2b, E4 ORF6, L1, L2, L3, L4 및 L5)는 아데노바이러스 입자의 바이러스 전염력 및 증식에 필요로 되는 비교대상 외 아데노바이러스 유전자 생성물의 존재하에서 배양될 수 있다. 이들 헬퍼 기능은 하나 이상의 헬퍼 구조체(예를 들어, 플라스미드 또는 바이러스) 또는 패키징 숙주 세포의 존재하에서 아데노바이러스 벡터를 배양함으로써 제공될 수 있다. 예를 들어, 1996년 5월 9일 공개되고, 본원에 참고로써 포함된 국제 특허 출원 WO96/13597의 "최소의" 인간 Ad 벡터의 제조에 대해 설명된 기술을 참조.
1. 헬퍼 바이러스
따라서, 미니유전자을 전달하는데 사용되는 바이러스 벡터의 유인원 아데노바이러스 유전자 함량에 의존하여, 헬퍼 아데노바이러스 또는 비-복제 바이러스 단편이 미니유전자를 함유하는 감염 재조합 바이러스 입자를 생성하는데 필요한 충분한 유인원 아데노바이러스 유전자 서열을 제공하기 위해 필요할 수 있다. 유용한 헬퍼 바이러스는 아데노바이러스 벡터 구조체에서 존재하지 않는 및/또는 벡터가 트랜스펙팅되는 패키징 셀 라인에 의해 발현되지 않는 선택된 아데노바이러스 유전자 서열을 함유한다. 한 구체예에서, 헬퍼 바이러스는 복제-결함이며, 상기 기술된 서열에 더하여 다양한 아데노바이러스 유전자를 함유한다. 이러한 헬퍼 바이러스는 E1-발현 셀 라인과 조합하여 바람직하게 사용된다.
헬퍼 바이러스는 또한 Wu et al, J. Biol . Chem ., 264:16985-16987 (1989); K. J. Fisher 및 J. M. Wilson, Biochem. J., 299:49 (1994년 4월 1일)에서 기술된 바와 같은 폴리-양이온 콘쥬게이트로 형성될 수 있다. 헬퍼 바이러스는 선택적으로 제 2 리포터 미니유전자를 함유할 수 있다. 다수의 이러한 리포터 유전자는 당업계에 공지되어 있다. 아데노바이러스 벡터에서 이식 유전자와 다른 헬퍼 바이러스 상의 리포터 유전자의 존재는 독립적으로 모니터링되는 Ad 벡터와 헬퍼 바이러스 둘 다를 허용한다. 이런 제 2 리포터는 정제 시 결과 재조합 바이러스와 헬퍼 바이러스 사이의 분리를 가능하게 하는데 사용된다.
2. 상보성 셀 라인
상기 기술된 어떤 유전자에서 결실된 재조합 유인원 아데노바이러스(Ad)를 발생시키기 위해, 바이러스의 복제 및 전염력에 필수적이라면, 결실된 유전자 영역의 기능은 헬퍼 바이러스 또는 셀 라인, 즉, 상보성 또는 패키징 셀 라인에 의해 재조합 바이러스에 공급되어야 한다. 많은 환경에서, 인간 E1을 발현시키는 셀 라인은 침팬지 Ad 벡터를 서로 보완하기 위해 사용될 수 있다. 본 발명의 침팬지 Ad 서열과 현재 이용가능한 패키징 세포에서 발견되는 인간 AdE1 서열 사이의 다양성에 기인하여, 현재 인간 E1-함유 세포의 사용이 복제 및 생성 과정 동안 복제-가능 아데노바이러스의 생성을 방지하기 때문에 이는 특히 유리하다. 그러나, 특정 환경에서, E1 유전자 생성물을 발현시키고 E1-결핍 유인원 아데노바이러스의 생성에 이용될 수 있는 셀 라인을 이용하는 것이 바람직할 것이다. 이러한 셀 라인은 기술되었다. 예를 들어, 미국 특허 6,083,716호 참조.
원한다면, 선택된 모 셀 라인에서 발현을 위한 프로모터의 전사 조절 하에서 SAdV28로부터 아데노바이러스 E1 유전자를 최소한으로 발현시키는 패키징 세포 또는 셀 라인을 발생시키기 위해 본원에 제공되는 서열을 이용할 수 있다. 유도성 또는 구성적 프로모터는 이 목적을 위해 사용될 수 있다. 이러한 프로모터의 예는 본 명세서의 어디에서나 상세하게 설명된다. 모 세포는 어떤 요망되는 SAdV28 유전자를 발현시키는 신규 셀 라인의 생성을 위해 선택된다. 제한 없이, 이러한 모 셀 라인은 특히 HeLa [ATCC Accession No. CCL 2], A549 [ATCC Accession No. CCL 185], HEK 293, KB [CCL 17], Detroit [예를 들어, Detroit 510, CCL 72] 및 WI-38 [CCL 75] 세포일 수 있다. 이들 셀 라인은 모두 American Type Culture Collection, 10801 University Boulevard, Manassas, Virginia 20110-2209로부터 이용가능하다. 다른 적당한 모 셀 라인은 다른 공급원으로부터 획득될 수 있다.
이러한 E1-발현 셀 라인은 재조합 유인원 아데노바이러스 E1 결실 벡터의 생성에서 유용하다. 추가적으로, 또 다르게는, 하나 이상의 유인원 아데노바이러스 유전자 생성물, 예를 들어, E1a, E1b, E2a, 및/또는 E4 ORF6을 발현시키는 셀 라인은 재조합 유인원 바이러스 벡터의 생성에서 사용되는 바와 같은 본질적으로 동일한 과정을 사용하여 구성될 수 있다. 이러한 셀 라인은 그런 생성물을 암호화하는 필수적 유전자에서 결실된 아데노바이러스를 서로 보완하기 위해, 또는 헬퍼-의존 바이러스(예를 들어, 아데노-관련 바이러스)의 패키징에 필요한 헬퍼 기능을 제공하기 위해 이용될 수 있다. 숙주 세포의 제조는 선택된 DNA 서열의 조합과 같은 기술을 수반한다. 이 조합은 통상적인 기술을 이용하여 수행될 수 있다. 이러한 기술은 폴리머라아제 연쇄 반응, 합성 방법, 및 원하는 뉴클레오티드 서열을 제공하는 어떤 다른 적당한 방법과 조합된, 잘 공지되어 있고 상기 인용한 Sambrook et al.에서 기술되는 cDNA 및 게놈 클로닝, 아데노바이러스 게놈의 중복 올리고뉴클레오티드 서열의 사용을 포함한다.
또 다른 대안으로, 필수적인 아데노바이러스 유전자 생성물이 아데노바이러스 벡터 및/또는 헬퍼 바이러스에 의해 트랜스에서 제공된다. 이러한 예에서, 적절한 숙주 세포는 원핵(예를 들어, 박테리아) 세포를 포함하는 어떤 생물학적 유기체, 및 곤충 세포, 효모 세포 및 포유동물 세포를 포함하는 진핵세포로부터 선택될 수 있다. 특히 바람직한 숙주 세포는, 제한 없이, A549, WEHI, 3T3, 10T1/2, HEK 293 세포 또는 PERC6 (이들 둘 다 기능적 아데노바이러스 E1을 발현시킨다) [Fallaux, FJ et al, (1998), Hum Gene Ther, 9:1909-1917], Saos, C2C12, L 세포, HT1080, HepG2 및 일차 섬유아세포, 인간, 원숭이, 마우스, 래트, 토끼 및 햄스터를 포함하는 포유동물로부터 유래된 간세포 및 근원세포와 같은 세포를 포함하는 어떤 포유동물 종 중에서 선택된다. 세포를 제공하는 포유동물 종의 선택은 본 발명을 제한하지 않으며; 포유동물 세포, 즉, 섬유아세포, 간세포, 종양 세포 등의 종류도 아니다.
3. 셀 라인의 바이러스 입자 및 트랜스펙션의 조합
일반적으로, 트랜스펙션에 의해 미니유전자를 포함하는 벡터를 전달할 때, 벡터는 약 1 x 104 세포 내지 약 1 x 1013 세포, 및 바람직하게는 약 105 세포에서 약 5 μg 내지 약 100 μg DNA, 및 바람직하게는 약 10 내지 약 50 μg DNA의 양으로 전달된다. 그러나, 선택된 벡터, 전달 방법 및 선택된 숙주 세포로서 고려하여, 숙주 세포에서 벡터 DNA의 상대적 양은 조절될 수 있다.
벡터는 네이키드 DNA, 플라스미드, 파지, 트랜스포존, 코스미드, 에피솜, 바이러스 등을 포함하여 당업계에 알려진 또는 상기 기재된 어떤 벡터일 수 있다. 벡터의 숙주 세포에 도입은 트랜스펙션, 및 감염을 포함하는 당업계에 공지된 또는 상기 기재된 바와 같은 어떤 수단에 의해 달성될 수 있다. 하나 이상의 아데노바이러스 유전자는 숙주 세포의 게놈에 안정적으로 통합되고, 에피솜으로서 안정적으로 발현되고, 또는 일시적으로 발현될 수 있다. 유전자 생성물은 모두 에피솜에서 일시적으로 발현되거나 안정적으로 통합될 수 있고, 또는 유전자 생성물의 일부는 안정적으로 발현되는 반면, 나머지는 일시적으로 발현될 수도 있다. 추가로, 각각의 아데노바이러스 유전자의 프로모터는 구성적 프로모터, 유도성 프로모터 또는 본래 아데노바이러스 프로모터로부터 독립적으로 선택될 수 있다. 프로모터는 예를 들어, 유기체 또는 세포의 특이적 생리학적 상태에 의해(즉, 분화상태에 의해 또는 복제 또는 정지 세포(quiescent cell)에서) 또는 외인성으로-첨가된 인자에 의해 조절될 수 있다.
숙주 세포에 분자(플라스미드 또는 바이러스)의 도입은 또한 당업자에게 공지되고, 본 명세서를 통해 논의되는 바와 같은 기술을 사용하여 수행될 수 있다. 바람직한 구체예에서, 표준 트랜스펙션 기술, 예를 들어, CaPO4 트랜스펙션 또는 전기천공법이 사용된다.
재조합 바이러스 입자를 생성하기 위해 아데노바이러스의 선택된 DNA 서열뿐만 아니라 이식 유전자 및 다른 벡터 요소의 다양한 중간체 플라스미드에의 조합, 및 플라스미드 및 벡터의 사용은 통상적인 기술을 사용하여 모두 달성된다. 이러한 기술은 문헌[Sambrook et al, 상기 인용]에서 기술되는 것과 같은 cDNA의 통상적인 클로닝 기술, 아데노바이러스 게놈의 중복 올리고뉴클레오티드 서열의 사용, 폴리머라아제 연쇄 반응, 및 원하는 뉴클레오티드 서열을 제공하는 어떤 적당한 방법을 포함한다. 표준 트랜스펙션 및 공동-트랜스펙션 기술, 예를 들어, CaPO4 침지 기법이 사용된다. 사용되는 다른 통상적인 방법은 바이러스 게놈의 상동 재조합, 한천중층에서 바이러스의 플라크, 신호 생성을 측정하는 방법 등을 포함한다.
예를 들어, 원하는 미니유전자-함유 바이러스 벡터의 구성 및 조합에 따라서, 벡터는 헬퍼 바이러스의 존재하에서 패키징 셀 라인 안으로 시험관 내에서 트랜스펙팅된다. 상동 재조합은 헬퍼와 벡터 서열 사이에서 발생하며, 이는 비리온 캡시드로 복제되고 패키징되는 벡터에서 아데노바이러스-이식 유전자 서열을 허용하여, 재조합 바이러스 벡터 입자를 초래한다. 이러한 바이러스 입자를 생성하는 현재의 방법은 트랜스펙션에 기초한다. 그러나, 본 발명은 이러한 방법에 제한되지 않는다.
결과 재조합 유인원 아데노바이러스는 선택된 이식 유전자가 선택된 세포로 이동하는데 유용하다. 패키징 셀 라인에서 성장한 재조합 바이러스에 의한 생체내 실험에서, 본 발명의 E1-결실 재조합 유인원 아데노바이러스 벡터는 이식 유전자를 비-유인원, 바람직하게는 인간, 세포에 이동시키는데 유용함을 증명한다.
IV. 재조합 아데노바이러스 벡터의 사용
재조합 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 기초 벡터는 시험관내, 생체 밖, 및 생체 내 인간 또는 비-유인원 수의과 환자에서 유전자 전달에 유용하다.
본원에 기술되는 재조합 아데노바이러스 벡터는 시험관내 이종성 유전자에 의해 암호화되는 생성물의 생성을 위한 발현 벡터로서 사용될 수 있다. 예를 들어, E1 결실의 위치로 삽입되는 유전자를 함유하는 재조합 아데노바이러스는 상기 기술한 바와 같은 E1-발현 셀 라인에 트랜스펙팅될 수 있다. 또 다르게는, 복제-가능 아데노바이러스는 다른 선택된 셀 라인에서 사용될 수 있다. 트랜스펙팅된 세포는 그 후 통상적인 방법으로 배양되고, 프로모터로부터 유전자 생성물을 발현시키기 위한 재조합 아데노바이러스를 허용한다. 유전자 생성물은 그 후 배양물로부터 단백질 분리 및 회수의 공지된 통상적인 방법에 의해 배양물 배지로부터 회수될 수 있다.
SAdV28 SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35-유래 재조합 유인원 아데노바이러스 벡터는 생체내 또는 생체밖에서 조차 선택되는 숙주 세포에 선택된 이식 유전자를 전달할 수 있는 효율적인 유전자 전달 비히클을 제공하며, 유기체는 하나 이상의 AAV 항원형에서 중화 항체를 가진다. 한 구체예에서, rAAV 및 세포는 생체밖에서 혼합되고; 감염 세포는 통상적인 방법을 사용하여 배양되며; 형질도입된 세포는 환자에 재주입된다. 이들 조성물은 치료적 목적 및 보호 면역을 유발하는 것을 포함하는 면역을 위한 유전자 전달에 특히 적합하다.
더 흔하게는, SAdV28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 재조합 아데노바이러스 벡터는 하기 기술되는 바와 같은 치료 또는 면역 분자의 전달을 위해 이용될 것이다. 본 발명의 재조합 아데노바이러스 벡터는 재조합 아데노바이러스 벡터의 반복 전달을 수반하는 요법에서 사용에 특히 적합하다는 것이 두 용도에 대해 용이하게 이해될 것이다. 이러한 요법은 전형적으로 바이러스 캡시드가 변형되는 일련의 바이러스 벡터의 전달을 수반한다. 바이러스 캡시드는 각각의 이후의 투여를 위해, 또는 특정 항원형 캡시드의 미리-선택된 수(예를 들어, 1, 2, 3, 4 또는 그 이상)의 투여 후 변형될 수 있다. 따라서, 요법은 제 1 유인원 캡시드와 함께 rAd의 전달, 제 2 유인원 캡시드와 함께 rAd의 전달, 및 제 3 유인원 캡시드와 함께 전달을 수반할 수 있다. 본 발명의 Ad 캡시드를 단독으로, 다른 것과 조합하여, 또는 다른 아데노바이러스와 조합하여(바람직하게는 면역적으로 비-교차반응임) 사용하는 다양한 다른 요법은 당업자에게 명백할 것이다. 선택적으로, 이러한 요법은 다른 비-인간 영장류 아데노바이러스, 인간 아데노바이러스, 또는 본원에 기술되는 것과 같은 인공 서열의 캡시드와 함께 rAd의 투여를 수반할 수 있다. 요법의 각 단계는 단일 Ad 캡시드로 일련의 주입(또는 다른 전달 경로) 후 다른 Ad 공급원으로부터 일련의 다른 캡시드의 투여를 수반할 수 있다. 또 다르게는, SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 벡터는 다른 바이러스 시스템, 비-바이러스 전달 시스템, 단백질, 펩티드, 및 다른 생물학적으로 활성인 분자를 포함하는 다른 비-아데노바이러스-매개 전달 시스템을 수반하는 요법에서 이용될 수 있다.
하기의 섹션은 본 발명의 아데노바이러스 벡터를 통해 전달될 수 있는 예시적인 분자에 초점을 맞출 것이다.
A. 치료 분자의 Ad-매개 전달
한 구체예에서, 상기-기술된 재조합 벡터는 유전자 치료를 위해 공개된 방법에 따라서 인간에 투여된다. 선택된 이식 유전자를 함유하는 유인원 바이러스 벡터는 환자에 투여될 수 있으며, 바람직하게는 생물학적으로 양립가능한 용액 또는 약학적으로 허용가능한 전달 비히클에서 현탁된다. 적당한 비히클은 멸균 식염수를 포함한다. 약학적으로 허용가능한 담체로 공지되고 당업자에게 잘 알려진 다른 수성 및 비-수성 등장 멸균 주사 용액 및 수성 및 비-수성 멸균 현탁액은 본 목적을 위해 사용될 수 있다.
유인원 아데노바이러스 벡터는 표적 세포를 형질도입하고 유전자 전달 및 발현의 충분한 수준을 제공하는데 충분한 양으로 투여되어, 지나친 불리함 없이 또는 의학적으로 허용가능한 생리적인 효과와 함께 치료적 이점을 제공하며, 이는 의학 분야의 당업자에 의해 결정될 수 있다. 투여의 통상적인 및 약학적으로 허용가능한 경로는, 제한되는 것은 아니지만, 망막에 직접적인 전달 및 다른 안구 전달 방법, 간에 직접적인 전달, 흡입, 비강내, 정맥내, 근육내, 기관내, 피하, 피내, 직장, 경구 및 다른 비경구 투여 경로를 포함한다. 투여 경로는, 원한다면, 이식 유전자 또는 질환에 따라서 조합 또는 조절될 수 있다. 투여 경로는 주로 치료되는 질환의 특성에 의존할 것이다.
바이러스 벡터의 투약은 치료되는 질환, 환자의 연령, 체중 및 건강상태와 같은 요인에 주로 의존할 것이고, 따라서 환자들 사이에서 다양할 수 있다. 예를 들어, 바이러스 벡터의 치료적으로 유효한 성인 인간 또는 수의과 투약량은 일반적으로 약 1 x 106 내지 약 1 x 1015 입자, 약 1 x 1011 내지 1 x 1013 입자, 또는 약 1 x 109 내지 1 x 1012 입자 바이러스의 농도를 함유하는 담체의 약 100 μL 내지 약 100 mL의 범위에 있다. 투약량은 동물의 크기 및 투여 경로에 의존하는 범위에 있을 것이다. 예를 들어, 근육내 주사에 대해 적당한 인간 또는 수의적 투약량(약 80 kg 동물)은 단일 자리에 대해 mL 당, 약 1 x 109 내지 약 5 x 1012 입자의 범위에 있다. 선택적으로, 투여의 다양한 자리는 전달될 수 있다. 다른 예에서, 적당한 인간 또는 수의적 투여는 경구 제형에 대해 약 1 x 1011 내지 약 1 x 1015 입자의 범위에 있을 수 있다. 당업자는 투여 경로, 및 재조합 벡터가 사용되기 위한 치료 또는 백신 용도에 따라서 이들 용량을 조절할 수 있다. 이식 유전자의 발현 수준, 또는 면역원, 순환 항체의 수준은 투약량 투여의 빈도를 결정하기 위해 모니터링 될 수 있다. 투여 빈도의 시간을 결정하기 위한 또 다른 방법은 당업자에게 용이하게 명백할 것이다.
선택적 방법 단계는 바이러스 벡터의 투여와 동시에, 또는 전 또는 후에 적당한 양의 짧은 작동 면역 조절자의 환자에서 공동-투여를 수반한다. 선택된 면역 조절자는 본 발명의 재조합 벡터에 대해 관련된 중화 항체의 형성을 억제할 수 있는 또는 벡터의 T 림프구 (CTL) 제거를 억제할 수 있는 약제로서 본원에 정의된다. 면역 조절자는 T 헬퍼 서브셋(TH1 또는 TH2)과 B 세포 사이에서 상호작용을 방해하여 중화 항체 형성을 억제할 수 있다. 또 다르게는, 면역 조절자는 TH1 세포와 CTL 사이의 상호작용을 억제하여 벡터의 CTL 제거의 발생을 감소시킬 수 있다. 다양한 유용한 면역 조절자 및 그것의 사용을 위한 투약량은, 예를 들어, Yang et al., J. Virol., 70(9) (Sept., 1996); 1996년 5월 2일 공개된 국제 특허 출원 번호 WO96/12406; 및 본원에 모두 참고로써 포함되는 국제 특허출원 번호 PCT/US96/03035에서 개시된다.
1. 치료 이식 유전자
이식 유전자에 의해 암호화되는 유용한 치료적 생성물은, 제한 없이, 인슐린, 글루카곤, 성장 호르몬(GH), 파라티로이드 호르몬(PTH), 성장 호르몬 방출 인자(GRF), 여포 자극 호르몬(FSH), 황체 형성 호르몬(LH), 인간 융모성 고나도트로핀(hCG), 혈관내피성장인자(VEGF), 엔지오포이에틴, 엔지오스태틴, 백혈구조혈성장인자 (GCSF), 에리스로포이에틴(EPO), 결합조직 성장인자(CTGF), 염기성 섬유아세포 성장인자 (bFGF), 산성 섬유아세포 성장인자(aFGF), 상피세포성장인자(EGF), 형질전환 성장인자 (TGF), 혈소판 유래 성장인자 (PDGF), 인슐린 성장 인자 1 및 II (IGF-I 및 IGF-II), TGF, 액티빈, 인히빈을 포함하는 형질전환 성장 인자 수퍼패밀리의 어떤 하나, 또는 어떤 뼈 형성 단백질(BMP) BMPs 1-15, 성장 인자의 헤레귤인/뉴레귤린/ARIA/neu 분화 인자(NDF) 패밀리 중 어떤 하나, 신경 성장인자(NGF), 뇌-유래 신경 친화성 인자(BDNF), 뉴로트로핀 NT-3 및 NT-4/5, 섬모 향신경성 인자(CNTF), 신경아교세포계 유래 신경영양 인자(GDNF), 뉴투린, 애그린, 세마포린/콜랩신의 패밀리 중 어떤 하나, 네트린-1 및 네트린-2, 간세포성장인자(HGF), 에프린, 노긴, 소닉 헤지호그 및 티로신 히드록실라아제를 포함하는 호르몬 및 성장 및 분화 인자를 포함한다.
다른 유용한 이식 유전자 생성물은, 제한 없이, 사이토카인 및 림포카인, 예로써, 트롬보포이에틴(TPO), IL-25를 통한 인터류킨(IL) IL-1(예를 들어, IL-2, IL-4, IL-12 및 IL-18을 포함), 단핵세포 화학유인물질 단백질, 백혈병 억제 인자, 과립성 백혈구 - 대식세포 집락 자극인자, 파스(Fas) 리간드, 종양 괴사 인자 및, 인터페론, 및 줄기 세포 인자, flk-2/flt3 리간드를 포함하는 면역 체계를 조절하는 단백질을 포함한다. 면역 체계에 의해 생성되는 유전자 생성물은 또한 본 발명에 유용하다. 이들은, 제한 없이, 면역글로불린 IgG, IgM, IgA, IgD 및 IgE, 키메라 면역글로불린, 인간화된 항체, 단일쇄 항체, T 세포 수용체, 키메라 T 세포 수용체, 단일쇄 T 세포 수용체, 클래스 I 및 클래스 II MHC 분자, 및 공학변형된 면역글로불린 및 MHC 분자를 포함한다. 유용한 유전자 생성물은 또한 상보적 조절 단백질, 막 보조 단백질(MCP), 붕괴 촉진인자(DAF), CR1, CF2 및 CD59를 포함한다.
또 다른 유용한 유전자 생성물은 호르몬, 성장 인자, 사이토카인, 림포카인, 조절 단백질 및 면역 체계 단백질에 대한 수용체 중 어떤 하나를 포함한다. 본 발명은 저밀도 리포단백질(LDL) 수용체, 고밀도 리포단백질(HDL) 수용체, 매우 낮은 밀도 리포단백질(VLDL) 수용체, 및 스캐빈저 수용체를 포함하는 콜레스테롤 조절을 위한 수용체를 포함한다. 본 발명은 또한 글루코코르티코이드 수용체 및 에스트로겐 수용체, 비타민 D 수용체 및 다른 핵 수용체를 포함하는 스테로이드 호르몬 수용체 수퍼패밀리의 멤버와 같은 유전자 생성물을 포함한다. 게다가, 유용한 유전자 생성물은 전사 인자, 예컨대, jun , fos, max, mad, 혈청반응인자(SRF), AP-1, AP2, myb, MyoD 및 마이오제닌, ETS-박스 함유 단백질, TFE3, E2F, ATF1, ATF2, ATF3, ATF4, ZF5, NFAT, CREB, HNF-4, C/EBP, SP1, CCAAT-박스 결합 단백질, 인터페론 조절 인자 (IRF-1), 윌름 종양 단백질, ETS-결합 단백질, STAT, GATA-박스 결합 단백질, 예컨대, GATA-3, 및 날개달린(winged) 나선형 단백질의 포크헤드(forkhead) 패밀리를 포함한다.
다른 유용한 생성물은, 카르바모일 합성효소 I, 오르니틴 트랜스카르바밀라아제, 아르기노숙시네이트 합성효소, 아르기노숙시네이트 리아제, 아르기나아제, 푸마릴아세트아세테이트 가수분해효소, 페닐알라닌 가수분해효소, 알파-1 안티트립신, 글루코오스-6-포스페이트, 포르포빌리노겐 디아미나아제, 인자 VIII, 인자 IX, 시스타티온 베타-합성효소, 가지사슬 케토산 데카르복실라아제, 알부민, 이소발레릴-coA 탈수소효소, 프로피오닐 CoA 카르복실라아제, 메틸 말로닐 CoA 무타아제, 글루타릴 CoA 탈수소효소, 인슐린, 베타-글루코시다아제, 파이루베이트 카르복실레이트, 간 포스포릴라아제, 포스포릴라아제 키나아제, 글리신 데카르복실라아제, H-단백질, T-단백질, 낭포성 섬유증 막단백질 조절자(CFTR) 서열, 및 디스트로핀 cDNA 서열을 포함한다.
다른 유용한 유전자 생성물은 비-천연적으로 발생하는 폴리펩티드, 예컨대, 삽입, 결실 또는 아미노산 치환을 함유하는 비-천연적으로 발생하는 아미노산 서열을 가지는 키메라 또는 하이브리드 폴리펩티드를 포함한다. 예를 들어, 단일-사슬 공학변형된 면역글로불린은 특정 면역타협 환자에서 유용할 수 있다. 비-천연적으로 발생하는 유전자 서열의 다른 종류는 표적의 과발현을 감소시키는데 사용될 수 있는 안티센스 분자 및 촉매적 핵산, 예를 들어 리보자임을 포함한다.
유전자 발현의 감소 및/또는 조절은 암 및 건선과 같이 이상증식 세포를 특징으로 하는 이상증식 질환의 치료에 특히 바람직하다. 표적 폴리펩티드는 정상 세포와 비교하여 이상증식 세포에서 배타적으로 또는 더 높은 수준으로 생성되는 폴리펩티드를 포함한다. 표적 항원은 myb, myc, fyn, 및 전좌 유전자 bcr/abl, ras, src, P53, neu, trk 및 EGRF과 같은 종양유전자에 의해 암호화되는 폴리펩티드를 포함한다. 표적 항원으로서 종양유전자 생성물에 더하여, 항-암 치료를 위한 표적 폴리펩티드 및 보호 요법은 B 세포 림프종에 의해 만들어지는 항체의 가변 영역 및 T 세포 림프종의 T 세포 수용체의 가변 영역을 포함하며, 일부 구체예에서, 이는 또한 자가면역 질병에 대한 표적 항원으로서 사용된다. 다른 종양-관련 폴리펩티드는 모노클로날 항체 17-1A 및 폴레이트 결합 폴리펩티드에 의해 인식되는 폴리펩티드를 포함하는 종양 세포에서 더 높은 수준으로 발견되는 폴리펩티드와 같은 표적 폴리펩티드로서 사용될 수 있다.
다른 적당한 치료 폴리펩티드 및 단백질은 세포 수용체 및 자기-관련 항체를 생성하는 세포를 포함하는 자가면역과 관련된 표적에 대하여 광범위 기초 보호 면역 반응을 부여함으로써 자가면역 질병 및 장애를 겪고 있는 개체를 치료하는데 유용할 수 있는 것들을 포함한다. T 세포 매개 자가면역 질병은 류마티스 관절염(RA), 다발성경화증(MS), 쇼그렌증후군, 유육종증, 인슐린 의존성 당뇨병(IDDM), 자가면역성 갑상샘염, 반응성 관절염, 강직성 척추염, 경피증, 다발성 근염, 건선, 혈관염, 베게너 육아종증, 크론병 및 궤양성 대장염을 포함한다. 각각의 이들 질병은 내인성 항원에 결합하고 자가면역 질병에 관련되는 면역 캐스캐이드를 시작하는 T 세포 수용체(TCR)를 특징으로 한다.
본 발명의 유인원 아데노바이러스 벡터는 특히 이식 유전자의 다양한 아데노바이러스-매개 전달이 요망되는 치료 요법에서, 예를 들어, 동일한 이식 유전자의 회복을 수반하는 요법에서 또는 다른 이식 유전자의 전달을 수반하는 요법과 조합하여 적합하다. 이러한 요법은 SAdV28 유인원 아데노바이러스 벡터의 투여 후, 동일한 항원형 아데노바이러스로부터의 벡터와 함께 재-투여를 수반할 수 있다. 특히 바람직한 요법은 SAdV28 유인원 아데노바이러스 벡터의 투여를 수반하며, 제 1 투여로 전달되는 벡터의 아데노바이러스 캡시드 서열의 공급원은 하나 이상의 이후의 투여에서 이용되는 바이러스 벡터의 아데노바이러스 캡시드 서열의 공급원과 다르다. 예를 들어, 치료 요법은 SAdV28 벡터의 투여 및 동일 또는 다른 항원형의 하나 이상의 아데노바이러스 벡터에 의한 반복 투여를 수반한다. 다른 예에서, 치료 요법은 아데노바이러스 벡터의 투여 후 제 1 전달 아데노바이러스 벡터에서 캡시드의 공급원과 다른 캡시드를 가지는 SAdV28 벡터에 의한 반복 투여, 및 선택적으로 투여 단계 전 벡터의 아데노바이러스 캡시드의 공급원과 동일한 또는, 바람직하게는 다른, 벡터에 의한 투여를 수반한다. 이들 요법은 SAdV28 유인원 서열을 사용하여 구성되는 아데노바이러스 벡터의 전달에 제한되지 않는다. 오히려, 이들 요법은, 제한 없이, 하나 이상의 SAdV28 벡터와 조합하여, 다른 유인원 아데노바이러스 서열(예를 들어, Pan9 또는 C68, C1, 등), 다른 비-인간 영장류 아데노바이러스 서열, 또는 인간 아데노바이러스 서열을 포함하는 다른 아데노바이러스 서열을 용이하게 이용할 수 있다. 이러한 유인원, 다른 비-인간 영장류 및 인간 아데노바이러스 항원형의 예는 본 문서에서 어디에서나 논의된다. 추가로, 이 치료 요법은 비-아데노바이러스 벡터, 비-바이러스 벡터, 및/또는 다양한 다른 치료적으로 유용한 화합물 또는 분자와 조합하여 SAdV28 아데노바이러스 벡터의 자발적 또는 순차적 전달을 수반할 수 있다. 본 발명은 이들 치료 요법에 제한되지 않으며, 다양한 것들이 당업자에게 용이하게 명확할 것이다.
B. 면역성 이식 유전자의 Ad-매개 전달
재조합 SAdV-28 벡터는 또한 면역성 조성물로서 사용될 수 있다. 본원에 사용된 바와 같이, 면역성 조성물은 체액(예를 들어, 항체) 또는 세포(예를 들어, 세포독성 T 세포) 반응이 포유동물, 및 바람직하게는 영장류에 전달 후 면역성 조성물에 의해 전달되는 이식 유전자 생성물에 고정되는 조성물이다. 재조합 유인원 Ad는 원하는 면역원을 암호화하는 그것의 아데노바이러스 서열 결실 유전자 중 어떤 것을 함유할 수 있다. 유인원 아데노바이러스는 인간 기원의 아데노바이러스와 비교하여 다른 동물 종에서 살아있는 재조합 바이러스 백신으로서 사용에 더 적합할 가능성이 있지만, 이러한 사용에 제한되는 것은 아니다. 재조합 아데노바이러스는 면역 반응의 유발에 결정적이고 병원체의 확산을 제한할 수 있다는 것이 확인된 항원(들)에 대한 어떤 병원체 및 cDNA에 대해 이용가능한 어떤 병원체에 대하여 예방 또는 치료 백신으로서 사용될 수 있다.
이러한 백신(또는 다른 면역원) 조성물은 상기 기술한 바와 간은 적당한 전달 비히클에서 제형화된다. 일반적으로 면역성 조성물에 대한 용량은 치료 조성물에 대해 상기 정의한 범위에 있다. 선택 유전자의 면역의 수준은, 만약에 있다면, 부스터에 대한 필요를 결정하기 위해 모니터링될 수 있다. 혈청에서 항체 타이터의 평가에 따라서, 선택적인 부스터 면역이 요망될 수 있다.
선택적으로, 본 발명의 백신 조성물은, 예를 들어, 보조제, 안정화제, pH 조절제, 보존제 등을 포함하는 다른 성분을 함유하기 위해 제형화될 수 있다. 이러한 성분은 백신 업계에서 당업자에게 잘 공지되어 있다. 적당한 보조제의 예는, 제한없이, 리포좀, 알륨, 모노포스포릴 지질 A, 및 어떤 생물학적으로 활성인 인자, 예로써, 사이토카인, 인터류킨, 케모킨, 리간드 및 최상으로는 그것의 조합을 포함한다. 특정의 이들 생물학적으로 활성인 인자는 생체내, 예를 들어, 플라스미드 또는 바이러스 벡터를 통해 발현될 수 있다. 예를 들어, 이러한 보조제는 항원만을 암호화하는 DNA 백신과 함께 프라이밍 시 발생되는 면역 반응과 비교하여, 항원-특이적 면역 반응을 향상시키기 위해 항원을 암호화하는 프라이밍 DNA 백신과 함께 투여될 수 있다.
재조합 아데노바이러스는 "면역원 양", 즉, 원하는 세포를 트랜스펙팅하기 위해 투여되는 경로에서 효과적이고, 면역 반응을 유발하기 위해 선택되는 유전자의 발현의 충분한 수준을 제공하는 양으로 투여된다. 보호 면역이 제공되는 경우, 재조합 아데노바이러스는 감염 및/또는 재발을 예방하는데 유용한 백신 조성물이 되는 것으로 고려된다.
또 다르게는, 또는 추가로, 본 발명의 벡터는 선택된 면역원에 대한 면역 반응을 유발하는 펩티드, 폴리펩티드 또는 단백질을 암호화하는 이식 유전자를 함유한다. 재조합 SAdV-28, AdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 벡터는 벡터에 의해 발현되는 삽입된 이종성 항원 단백질에서 세포 용해 T 세포 및 항체를 유발할 때 매우 효율적인 것으로 기대된다.
예를 들어, 면역원은 다양한 바이러스 과로부터 선택될 수 있다. 면역 반응이 바람직한 바이러스 패밀리의 예는, 보통 감기의 약 50%의 경우를 초래하는 리노바이러스 속; 폴리오바이러스, 콕사키 바이러스, 에코바이러스 및 A형 간염 바이러스와 같은 인간 엔테로바이러스를 포함하는 엔테로바이러스 속; 및 주로 비-인간 동물에서 발 및 구강 질병을 초래하는 압소바이러스 속을 포함하는 피코르나바이러스 과를 포함한다. 바이러스의 피코르나바이러스 과 내에서, 표적 항원은 VP1, VP2, VP3, VP4, 및 VPG를 포함한다. 다른 바이러스 과는 바이러스의 노워크(Norwalk) 군을 포함하고, 유행성 위장염의 중요한 감염인자인 칼시바이러스 과를 포함한다. 인간 및 비-인간 동물에서 면역 반응을 유발하기 위한 표적 항원에서 사용에 바람직한 또 다른 바이러스 과는 토가바이러스 과이며, 이는 신드비스 바이러스, 로스리버 바이러스 및 베네수엘라, 동부형&서부형 마뇌염, 및 루벨라 바이러스를 포함하는 루비바이러스를 포함하는 알파바이러스 속을 포함한다. 플라비비리다에과는 뎅기열, 황열, 일본 뇌염, 세인트루이스 뇌막염 및 진드기매개 바이러스를 포함한다. 다른 표적 항원은 C형 간염 또는 코로나바이러스과로부터 발생될 수 있으며, 이는 다수의 비-인간 바이러스, 예컨대, 전염성 기관지염(가금류), 돼지 전염성 위장염 바이러스(돼지), 돼지 혈구응집성 뇌척수염 바이러스(돼지), 고양이 전염성 복막염 바이러스(고양이), 고양이 장 코노나바이러스(고양이), 개 코로나바이러스(개), 및 인간 호흡 코로나바이러스를 포함하며, 이는 보통의 감기 및/또는 비-A, B 또는 C형 간염을 야기할 수 있다. 코로나바이러스과 내에서, 표적 항원은 E1 (또한 M 또는 매트릭스 단백질로 불림), E2 (또한 S 또는 스파이크 단백질로 불림), E3 (또한 HE 또는 헤마그글루틴-엘터로오스로 불림) 글리코단백질(모든 코로나바이러스에 존재하지 않음) 또는 N(뉴클레오캡시드)을 포함한다. 또 다른 항원은 베시큘로바이러스속(예를 들어, 소수포형 구내염 바이러스), 및 리사 바이러스속(예를 들어, 광견병)을 포함하는 랩도 바이러스과를 표적으로 할 수 있다.
랩도바이러스 과 내에서, 적당한 항원은 G 단백질 또는 N 단백질로부터 유래될 수 있다. 마르부르크 및 에볼라 바이러스와 같은 출혈열 바이러스를 포함하는 필로바이러스 과는 항원의 적당한 공급원일 수 있다. 파라믹소바이러스 과는 파라인플루엔자 바이러스 타입 1, 파라인플루엔자 바이러스 타입 3, 소 파라인플루엔자 바이러스 타입 3, 루불라바이러스(멈프스 바이러스), 파라인플루엔자 바이러스 타입 2, 파라인플루엔자 바이러스 타입 4, 뉴캐슬병 바이러스(닭), 우역, 홍역 및 개디스템퍼를 포함하는 모르비리바이러스, 호흡기 세포융합 바이러스를 포함하는 뉴모바이러스를 포함한다. 인플루엔자 바이러스는 오소믹소바이러스 과 내로 분류되며 적당한 항원(예를 들어, HA 단백질, N1 단백질)의 공급원이다. 분야바이러스 과는 분야바이러스 속(캘리포니아뇌염, La Crosse), 플레보바이러스(리프트 밸리열), 한타바이러스 (퓨어말라(puremala)는 헤마하긴(hemahagin) 열 바이러스이다), 나이로바이러스(진드기증(Nairobi sheep disease)) 및 다양한 미지정 분야바이러스를 포함한다. 아레나바이러스 과는 LCM 및 라사열 바이러스에 대한 항원의 공급원을 제공한다. 레오 바이러스 과는 레오바이러스, 로타바이러스(어린이에게서 급성위장염을 야기한다), 오르비바이러스, 및 컬티바이러스(콜로라도진드기열, 레봄보(Lebombo) (인간), 말 뇌증, 청설병) 속을 포함한다.
레트로바이러스 과는 고양이 백혈병 바이러스, HTLVI 및 HTLVII, 렌티바이러스(인간 면역결핍 바이러스(HIV), 유인원 면역결핍 바이러스(SIV), 고양이 면역부전 바이러스(FIV), 말 전염성 빈혈 바이러스 및 스푸마바이러스를 포함)로서 인간 및 수의과 질병을 포함하는 옹코리비리날(oncorivirinal) 아과를 포함한다. 렌티바이러스 중에서, 많은 적당한 항원이 기술되었고 용이하게 선택될 수 있다. 적당한 HIV 및 SIV 항원의 예는, 제한 없이, gag, pol, Vif, Vpx, VPR, Env, Tat, Nef, 및 Rev 단백질뿐만 아니라 그것의 다양한 단편을 포함한다. 예를 들어, Env 단백질의 적당한 단편은 gp120, gp160, gp41과 같은 어떤 그것의 서브유닛, 또는 그것의 더 작은 단편, 예를 들어, 길이에 있어 적어도 약 8개의 아미노산을 포함할 수 있다. 유사하게, tat 단백질의 단편이 선택될 수 있다. [미국 특허 5,891,994호 및 미국 특허 6,193,981호 참조] 또한, D.H. Barouch et al, J. Virol., 75(5):2462-2467 (March 2001년 3월), 및 R.R. Amara, et al, Science, 292:69-74 (2001년 4월 6일)에서 기술되는 HIV 및 SIV 단백질 참조. 다른 예에서, HIV 및/또는 SIV 면역성 단백질 또는 펩티드는 융합 단백질 또는 다른 면역성 분자를 형성하기 위해 사용될 수 있다. 예를 들어, 2001년 8월 2일 공개된 WO 01/54719, 및 1999년 4월 8일 공개된 WO 99/16884에서 기술되는 HIV-1 Tat 및/또는 Nef 융합 단백질 및 면역 요법을 참조. 본 발명은 HIV 및/또는 SIV 면역원성 단백질 또는 본원에 기술되는 펩디드로 제한되지 않는다. 게다가, 이들 단백질에서 다양한 변형이 기술되었고, 또는 당업자에 의해 용이하게 만들어질 수 있었다. 예를 들어, 미국 특허 5,972,596에서 기술되는 변형된 구역 단백질(gag protein)을 참조. 추가로, 어떤 요망되는 HIV 및/또는 SIV 면역원은 단독으로 또는 조합하여 전달될 수 있다. 이러한 조합은 단일 벡터 또는 다중 벡터로부터 발현을 포함할 수 있다. 선택적으로, 다른 조합은 단백질 형태에서 하나 이상의 면역원의 전달과 함께 하나 이상의 발현된 면역원의 전달을 수반할 수 있다. 이러한 조합은 하기에서 더욱 상세하게 논의된다.
파포바바이러스 과는 폴리오마바이러스 아과(BKU 및 JCU 바이러스) 및 파필로마바이러스 아과(암 또는 유두종의 악성 진행과 관련)를 포함한다. 아데노바이러스 과는 호흡기 질병 및/또는 장염을 야기하는 바이러스(EX, AD7, ARD, O. B.)를 포함한다. 파보바이러스 과 고양이 파보 바이러스(고양이 장염), 고양이 범백혈구감소증 바이러스, 개 파보바이러스 및 돼지 파보바이러스. 헤르페스바이러스 과는 심플렉스바이러스 속 (HSVI, HSVII), 바리셀로바이러스 (가성광견병, 바리셀라-조스터 바이러스)를 포함하는 알파헤르페스바이러스 아과 및 시토메갈로 바이러스(HCMV, 무로메갈로바이러스)를 포함하는 베타헤르페스바이러스 아과 및 림포크립토바이러스 속, EBV (버킷 임파종(Burkitts lymphoma)), 전염성비기관염, 마렉병 바이러스, 및 라디노바이러스를 포함하는 감마헤르페스바이러스 아과를 포함한다. 수두 바이러스과는 오르토폭스바이러스(바리올라 (두창) 및 백시니아 (우두)), 파라폭스바이러스, 아비폭스바이러스, 카프리폭스바이러스, 레포리폭스바이러스, 수이폭스바이러스 속을 포함하는 초르도폭스바이러스아과, 및 엔토모폭스바이러스 아과를 포함한다. 헤파드나바이러스 과는 B형 간염 바이러스를 포함한다. 적당한 항원의 공급원일 수 있는 한 미분류 바이러스는 델타감염 바이러스이다. 또 다른 바이러스 공급원은 조류 전염성 훼브리셔스낭병 바이러스 및 돼지 호흡기 생식기 증후군 바이러스를 포함할 수 있다. 알파바이러스 과는 말동맥염바이러스 및 다양한 뇌염바이러스를 포함한다.
다른 병원체에 대한 인간 또는 비-인간 동물을 면역화하는데 유용한 면역원은, 예를 들어, 인간 및 비-인간 척추동물을 감염시키는 박테리아, 진균, 기생충미생물 또는 다세포 기생충, 또는 암 세포 또는 종양 세포를 포함한다. 박테리아 병원체의 예는 폐렴쌍구균; 포도상구균; 및 연쇄상구균을 포함하는 병원체의 그램양성 구균을 포함한다. 병원체의 그램-음성 구균은 뇌척수막염균; 임균을 포함한다. 병원체의 장 그램-음성 간균은 장내세균(enterobacteriaceae); 슈도모나스, 아시네토박테리아 및 에이케넬라; 멜리오이도시스; 살모넬라; 시겔라; 헤모필루스; 모락셀라; H. 듀크레이(무른 궤양을 야기함); 브루셀라균; 프란시셀라 툴라렌시스균(툴라레미아를 야기); 예르시니아(파스튜렐라); 모닐리포르미스사슬막대균 및 나선균을 포함하고; 그램-양성 간균은 리스테리아모노사이토제네스; 돈단독균(erysipelothrix rhusiopathiae); 코리네박테리움 디프테리아(디프테리아); 콜레라; 탄저균 (탄저병); 도노반증(서혜육아종); 및 바르토넬라증을 포함한다. 병원성 혐기성 세균에 의해 야기되는 질병은 파상풍; 보툴리즘; 다른 클로스트리디아; 결핵; 나병; 및 다른 마이코박테리아를 포함한다. 병원성 스피로헤타병은 매독; 트레포네마병: 매종, 핀타 및 풍토병성 매독; 및 렙토스피라병을 포함한다. 더 고등의 병원체 박테리아 및 병원성 진균에 의해 야기되는 다른 감염은 방선균증; 노카르디아증; 효모균증, 분아진균증, 히스토플라스마증 및 콕시디오이데스 진균증; 칸디다증, 아스페르길루스증, 및 뮤코르 진균증; 스포로트릭스증; 파라콕시디오이드마이세스증, 페트리엘리듐증, 토룰롭시스증, 균종 및 색소진균증; 및 피부사상균증을 포함한다. 리케차감염은 발진티푸스, 로키산 홍반열, Q열, 및 리켓치아폭스를 포함한다. 마이코플라스마 및 클라미디아 감염의 예는: 마이코플라즈마 뉴모니아; 서혜 림프 육아종; 앵무새병; 및 주산기 클라미디아 감염을 포함한다. 병원성 진핵생물은 병원성 원생동물 및 장내 기생충을 포함하고, 이에 의해 생성되는 감염은: 아메바성 감염; 말라리아; 리슈만편모충증; 트리파노소마증; 톡소플라스마증; 폐포자충(Pneumocystis carinii); 트리칸스(Trichans); 톡소포자충(Toxoplasma gondii) ; 바베스열원충증; 지알디아증; 선모충병; 필라리아병; 주혈흡충병; 선충; 흡충 또는 요행; 및 촌충류(촌충) 감염을 포함한다.
다수의 이들 유기체 및/또는 이에 의해 생성되는 독소는 생물학적 공격에서 사용을 위한 가능성을 가지는 약제로서 질병 대책 센터(Centers for Disease Control)[(CDC), Department of Heath and Human Services, USA]에 의해 확인되었다. 예를 들어, 일부의 이들 생물학적 약제는 탄저균 (탄저병), 클로스트리디움 보툴리늄 및 그것의 독소(보툴리즘), 페스트균(Yersinia pestis)(흑사병), 대두창(두창), 프란키셀라 툴라렌시스(Francisella tularensis)(툴라레미아), 및 바이러스성 출혈열[필로바이러스(예를 들어, Ebola, Marburg], 및 아레나바이러스[예를 들어, Lassa, Machupo])를 포함하며, 이들 모두는 현재 카테고리 A 약제로서 분류되며; 콕시엘라 부르네티(Q 열); 브루셀라 종(브루셀라병), 비저균(Burkholderia mallei)(마비저), 부르코홀데리아 슈도 말레이(Burkholderia pseudomallei)(유비저), 피마자 및 그것의 독소(리신 독소), 클로스트리듐 균(clostridium perfringen) 및 그것의 독소(엡실론 독소), 포도상구균 종 및 그것의 독소(엔테로톡시 B), 클라미디아 시타시(앵무새병), 물의 안전성 위협(예를 들어, 비브리오콜레라, 크립토스포리듐 파르붐), 발진티푸스(리케챠 포와제키(Rickettsia powazekii)), 및 바이러스성뇌염(알파바이러스, 예를 들어, 베네수엘라마뇌염; 동부형마 뇌막염; 서부형 마뇌염)를 포함하고; 이들 모두는 카테고리 B 약제로서 분류되고; 니판 바이러스 및 한타바이러스를 포함하고, 이것은 카테고리 C 약제로서 분류된다. 게다가, 이렇게 분류 또는 다르게 분류되는 다른 유기체는 장래의 목적을 위해 확인 및/또는 사용될 수 있다. 본원에서 기술되는 바이러스 벡터 및 다른 구조체는 이들 유기체, 바이러스, 그것의 독소 또는 다른 부산물로부터, 이들 생물학적 약제에 의한 감염 또는 다른 역반응을 예방 및/또는 치료할 항원을 전달하는데 유용하다는 것이 이해될 것이다.
T 세포의 가변 영역에 대해 면역원을 전달하는 SAdV-28 벡터의 투여는 이들 T 세포를 제거하기 위해 CTL을 포함하는 면역반응을 일으키는 것으로 예상된다. RA에서, 질병에 수반되는 TCR의 몇몇의 특정 가변 영역은 특성이 기술되었다. 이들 TCR은 V-3, V-14, V-17 및 Vα-17을 포함한다. 따라서, 적어도 하나의 이들 폴리펩티드를 암호화하는 핵산 서열의 전달은 RA에 수반된 T 세포를 표적화할 면역반응을 유발할 것이다. MS에서, 질병에 수반된 TCR의 몇몇 특정 가변 영역은 특성이 기술되었다. 이들 TCR은 V-7 및 Vα-10을 포함한다. 따라서, 적어도 하나의 이들 폴리펩티드를 암호화하는 핵산 서열의 전달은 MS에 수반되는 T세포를 표적화할 면역반응을 유발할 것이다. 경피증에서, 질병에 수반된 TCR의 몇몇 특정 가변 영역은 특성이 기술되었다. 이들 TCR은 V-6, V-8, V-14 및 Vα-16, Vα-3C, Vα-7, Vα-14, Vα-15, Vα-16, Vα-28 및 Vα-12을 포함한다. 따라서, 적어도 하나의 이들 폴리펩티드를 암호화하는 재조합 유인원 아데노바이러스의 전달은 경피증에 수반된 T 세포를 표적화할 면역 반응을 일으킬 것이다.
C. Ad-매개 전달 방법
선택된 유전자의 치료 수준, 또는 면역의 수준은, 만약에 있다면, 부스터에 대한 필요를 결정하기 위해 모니터링될 수 있다. 혈청에서 CD8+ T 세포 반응, 또는 선택적으로 항체 타이터의 평가에 따라서, 선택적인 부스터 면역화가 요망될 수 있다. 선택적으로, 재조합 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 벡터는 단일 투여에서 또는 예를 들어, 다른 활성 성분을 수반하는 요법 또는 치료의 과정과 조합하는 다양한 요법 또는 프라임-부스트 요법에서 전달될 수 있다. 다양한 이러한 요법은 당업계에서 기술되었고 용이하게 선택될 수 있다.
예를 들어, 프라임-부스트 요법은 일차 면역 체계로 DNA(예를 들어, 플라스미드) 기초 벡터를, 이차의 부스터로 이러한 항원을 암호화하는 서열을 전달하는 단백질 또는 재조합 바이러스와 같은 일반적인 항원의 투여를 수반할 수 있다. 예를 들어, 참고로써 포함되는 2000년 3월 2일 공개된 WO 00/11140 참조. 또 다르게는, 면역 요법은 항원, 또는 단백질을 전달하는 벡터(바이러스 또는 DNA-기초)에 대한 면역 반응을 촉진하기 위해 재조합 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 벡터의 투여를 수반할 수 있다. 또 다른 대안으로, 면역 요법은 단백질의 투여 후 항원을 암호화하는 벡터와 함께 부스터를 수반한다.
한 구체예에서, 상기 항원을 전달하는 플라스미드 DNA 벡터를 전달한 후 재조합 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 벡터로 부스팅함으로써 선택된 항원에서 면역반응을 프라이밍하고 부스팅하는 방법이 기술된다. 한 구체예에서, 프라임-부스트 요법은 프라임 및/또는 부스트 비히클로부터 멀티단백질의 발현을 수반한다. 예를 들어, HIV 및 SIV에 대한 면역 반응을 발생시키는데 유용한 단백질 서브유닛의 발현에 대한 멀티단백질 요법을 기술하는 R. R. Amara, Science, 292:69-74 (2001년 4월 6일) 참조. 예를 들어, DNA 프라임은 단일 전사로부터 Gag, Pol, Vif, VPX 및 Vpr 및 Env, Tat, 및 Rev를 전달할 수 있다. 또 다르게는, SIV Gag, Pol 및 HIV-1 Env는 재조합 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 아데노바이러스 구조체에서 전달된다. 또 다른 요법은 WO 99/16884 및 WO 01/54719에서 기술된다.
그러나, 프라임-부스트 요법은 HIV에 대한 면역 또는 이들 항원의 전달에 제한되지 않는다. 예를 들어, 프라이밍은 제 1 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 벡터에 의한 전달단계 후 제 2 Ad 벡터로, 또는 단백질 형태에서 항원 그 자체를 함유하는 조성물과 함께 부스팅하는 단계를 수반할 수 있다. 한 예에서, 프라임-부스트 요법은 항원이 유래된 바이러스, 박테리아 또는 다른 유기체에 대한 보호 면역 반응을 제공할 수 있다. 다른 구체예에서, 프라임-부스트 요법은 치료제가 투여되는 질환의 존재의 검출을 위한 통상적인 분석을 사용하여 측정될 수 있는 치료효과를 제공한다.
프라이밍 조성물은 요망되는 면역 반응이 표적화되는 항원에 따라서 용량 의존적 방법으로 다양한 자리에 투여될 수 있다. 주사(들)의 양 또는 위치 또는 약학적 담체는 제한되지 않는다. 오히려, 요법은 이들 각각이 매 시간마다, 매일, 주마다 또는 매월 또는 매년마다 투여되는 단일 용량 또는 투약량을 포함할 수 있는 프라이밍 및/또는 부스팅 단계를 수반할 수 있다. 예로서, 포유동물은 담체에서 약 10 μg 내지 약 50 μg의 플라스미드를 함유하는 하나 이상의 용량을 수용할 수 있다. DNA 조성물의 바람직한 양은 약 1 μg 내지 약 10,000 μg의 DNA 벡터의 범위에 있다. 투약량은 피험자 체중 당 1 μg 내지 1000 μg DNA로 다양할 것이다. 전달의 양 또는 자리는 포유동물의 동일성 및 질환에 기초하여 바람직하게 선택된다.
포유동물에 대한 항원의 전달에 적당한 벡터의 투약 단위는 본원에서 기술된다. 벡터는 등장 식염수; 등장 염 용액 또는 이러한 투여에서 당업자에게 명백할 다른 제형과 같은 약학적으로 또는 생리학적으로 허용가능한 담체로 현탁 또는 용해됨으로써 투여를 위해 제조된다. 적절한 담체는 당업자에게 명백할 것이고 투여 경로의 상당 부분에 의존할 것이다. 본원에 기술되는 조성물은 상기 기술된 경로에 따라서, 서방성 제형으로 생체분해가능한 생체적합성 폴리머를 사용하여, 또는 미셀, 겔 및 리포좀을 사용하는 현장 전달에 의해 포유동물에 투여될 수 있다. 선택적으로, 프라이밍 단계는 또한 본원에 기술되는 바와 같은 프라이밍 조성물, 적당한 양의 보조제와 함께 투여하는 단계를 포함한다.
바람직하게는, 부스팅 조성물은 포유동물 피험자에 대해 프라이밍 조성물을 투여 후 약 2 내지 약 27주에 투여된다. 부스팅 조성물의 투여는 프라이밍 DNA 백신에 의해 투여되는 동일한 항원을 함유하는 또는 전달할 수 있는 부스팅 조성물의 유효량을 사용하여 수행된다. 부스팅 조성물은 동일한 바이러스 공급원(예를 들어, 본 발명의 아데노바이러스 서열) 또는 다른 공급원으로부터 유래된 재조합 바이러스 벡터로 구성될 수 있다. 또 다르게는, "부스팅 조성물"은 프라이밍 DNA 백신에서, 그러나 조성물이 숙주에서 면역 반응을 유발하는 단백질 또는 펩티드의 형태로 암호화되는 바와 같은 동일한 항원을 함유하는 조성물일 수 있다. 다른 구체예에서, 부스팅 조성물은 포유동물 세포에서 그것의 발현을 지시하는 조절 서열, 예를 들어, 잘-공지된 박테리아 또는 바이러스 벡터와 같은 벡터의 제어하에서 항원을 암호화하는 DNA 서열을 함유한다. 부스팅 조성물의 일차적 요건은 조성물의 항원이 프라이밍 조성물에 의해 암호화되는 동일 항원, 또는 교차-반응 항원이다.
다른 구체예에서, SAdV-28 벡터는 또한 다양한 다른 면역 및 치료 요법에서 사용을 위해 적합하게 된다. 이러한 요법은 다른 항원형 캡시드의 Ad 벡터와 함께 동시에 또는 순차적으로 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 벡터의 전달, SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 벡터가 동시에 또는 순차적으로 비-Ad 벡터와 함께 전달되는 요법, SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 벡터가 동시에 또는 순차적으로 단백질, 펩티드 및/또는 다른 생물학적으로 유용한 치료 또는 면역원성 화합물과 함께 전달되는 요법을 수반할 수 있다. 이러한 사용은 당업자에게 용이하게 명백할 것이다.
하기 실시예는 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 및/또는 SAdV-35 및 대표적인 재조합 SAdV-28 벡터의 구성의 클로닝을 예시한다. 이들 실시예는 단지 예시적이며, 본 발명의 범주를 제한하지 않는다.
실시예 1 - 유인원 아데노바이러스의 분리.
University of Louisiana New Iberia Research Center, 4401 W. Admiral Doyle Drive, New Iberia, Louisiana, USA에서 침팬지 집단, 및 Michael E. Keeling Center for Comparative Medicine and Research, University of Texas M. D. Anderson Cancer Center, Bastrop, Texas, USA에서 침팬지 집단으로부터 채변 샘플을 얻었다. 행크스 평형화 염 용액의 현탁액에서 침팬지 채변 샘플로부터의 상청액을 0.2 미크론 실린지 필터를 통해 멸균 여과하였다. 100 μl의 각각의 여과된 샘플을 인간 셀 라인 A549 배양물에 접종하였다. 이들 세포를 10% FBS, 1% Penn-Strep 및 50μg/ml 겐타마이신과 함께 Ham's F12에서 성장시켰다. 배양물에서 약 1 내지 2주 후, 시각적 세포변성 효과(CPE)는 몇몇의 접종물과 함께 세포 배양물에서 명백하였다. 아데노바이러스를 아데노바이러스 정제를 위한 표준 공개된 염화세슘 기울기 기술을 사용하여 A549 세포에서 배양물로부터 정제하였다. 정제한 아데노바이러스로부터 DNA를 분리하였고 Qiagen Genomic services, Hilden, Germany에 의해 완전히 서열화하였다.
바이러스 DNA 서열의 계통발생적 분석에 기초하여, 아데노바이러스 지정된 유인원 아데노바이러스 27 (SAdV-27), 유인원 아데노바이러스 28 (SAdV-28), 유인원 아데노바이러스 29 (SAdV-29), 유인원 아데노바이러스 32 (SAdV-32), 유인원 아데노바이러스 33, (SAdV-33) 및 유인원 아데노바이러스 35 (SAdV-35)를 인간 아군 B로서 동일 아군 내가 되도록 결정하였다.
벡터를 만들기 위해 사용되는 방법은 우선 전체 E1-결실 아데노바이러스 벡터의 박테리아 플라스미드 분자 클론을 만든 후 E1 보완 셀 라인 HEK 293에 플라스미드 DNA의 트랜스펙션을 하여 바이러스 벡터를 구제한다.
E1-결실 아데노바이러스 벡터의 분자 클론을 만들기 위해, 희소-절단(rare-cutting) 제한 효소 I-CeuI 및 PI-SceI에 대한 인식 자리가 E1 결실 대신에 삽입된다면, E1-결실 아데노바이러스의 플라스미드 분자 클론을 우선 만들었다. I-CeuI 및 PI-SceI에 의해 측면에 위치되고, 이들 제한 효소를 사용하여 절단되는 발현 카세트는 E1-결실 아데노바이러스 플라스미드 클론에 연결된다. E1 결실 대신에 요망되는 발현 카세트를 포함하는 플라스미드 아데노바이러스 분자 클론을 재조합 아데노바이러스 벡터를 구제하기 위해 HEK 293 세포에 트랜스펙팅하였다. 트랜스펙션 후 구제는 제한효소 분해에 의해 플라스미드로부터 선형 아데노바이러스 게놈을 우선 방출함으로써 가능하게 되는 것이 발견되었다.
실시예 2 - E1-결핍 재조합 아데노바이러스 벡터의 유도를 가능하게 하기 위한 유인원 아군 B 아데노바이러스 27, -28, -29, -32, -33, 및 -35에 기초한 E1 결핍 플라스미드 분자 클론의 구성
AdC1(SAdV-21)을 경험하는 발명자 뿐만 아니라 두 개의 공개된 기록은 아군 B 아데노바이러스에서 E1 결핍이 HEK 293 세포에서 Ad5 E1 유전자에 의해 보완되지 않는다는 것을 나타내기 때문에, 종 B 아데노바이러스 SAdV-27, SAdV-28, SAdV-29, SAdV-32, 및 SAdV-35를 기초로 하는 벡터를 AdC1을 사용하는 하이브리드 아데노바이러스 벡터를 구성하는 이전의 기술된 전략을 기초로 구성하였고[Roy et al., J Virol. Methods. (2007) 141, 14-21 ; Roy et al., J Gen Virol. (2006) 87, 2477-2485], 키메라 구조체의 왼쪽 및 오른쪽 말단은 침팬지 아데노바이러스 Pan 5로부터 유래된다(a.k.a. 유인원 아데노바이러스 22).
아군 B 아데노바이러스 벡터의 구성을 위한 출발 플라스미드는 2005년 1월 6일 공개된 WO 2005/001103 A3에서 기술되는 바와 같은 AdC1 키메라 벡터-pPan5CldelRI의 구조체에서 중간체로서 구성한 것이었다. 플라스미드 pPan5C1delRI는 EcoRI 제한 자리 사이에서 내부로 결핍된 E1 -결핍 키메라 Pan 5 (SAdV-22) 및 Ad C1 (SAdV-21) 아데노바이러스 게놈을 포함한다. 추가적으로, 희소-절단 제한 효소 I-CeuI 및 PI-Seel에 대한 인식 자리는 이식 유전자 카세트의 용이한 삽입을 가능하게 하기 위해 E1 결핍 대신에 존재한다.
A. 표준 분자 생물학 기술을 사용하는 SAdV -27에 기초한 E1-결핍 플라스미드 분자 클론의 구성
두 개의 공개된 기록 및 AdC1을 경험한 발명자가 아군 B 아데노바이러스에서 E1 결핍이 HEK 293 세포에서 Ad5 El 유전자에 의해 보완되지 않음이 나타냈기 때문에, 하이브리드 아데노바이러스는 AdC1을 사용하는 전략을 기초로 발생되었고[Roy et al., J Virol. Methods. (2007) 141, 14-21 ; Roy et al., J Gen Virol. (2006) 87, 2477-2485], 키메라 구조체의 왼쪽 및 오른쪽 끝은 침팬지 아데노바이러스 Pan 5로부터 유래된다(a.k.a. 유인원 아데노바이러스 22).
1. 링커의 삽입
pPan5CldelRI (ClaI과 EcoRI 사이)로부터 Ad C1 부분을 올리고머 SAD27 top 및 SAD27 bot를 어닐링함으로써 만든 DNA 링커로 대체하였다. SEQ ID NO: 198: SAD27 top - CGCGCCGAGCATTCATGCTTGTACGTA- CCCACGCACAGCTTTAAACATTTG 및 SEQ ID NO: 199: SAD27 bot - AATTCAAATGTTTAAAGCTGTGCGTGGGTACGTACAAGCATGAATGCTCGG. 이 플라스미드는 pC5endsSAD27 올리고이다.
2. PCR
플라스미드 pC5endsSAD27 올리고를 AscI 및 EcoRI로 분해하였고, PCR-발생된 3873 bp 단편(또는 AscI 및 EcoRI로 분해함)을 pC5endsSAD27PCR을 만들기 위해 클로닝하였다. PCR 단편을 제조업자의 설명서에 따라서 주형으로서 SAdV-27 바이러스 DNA를 사용하고, 프라이머 SAD27 Asc 및 SAD27 RI를 사용하고, NEB Phusion PCR 키트를 사용하여 발생시켰다. PCR 프라이머는 AscI 및 EcoRI 자리를 각각 함유하였다.
SEQ ID NO: 200: SAD27 Asc - TACCACCAGCGGCGCGCCAGACATCAAG
SEQ ID NO: 201: SAD27 RI - AAATGGAATTCAAATGTTTAAAGCTGTG
3. SAdV-27로부터 AscI (7951 - 17458) 단편의 삽입.
플라스미드 pC5endsSAD27PCR를 AscI로 분해하였고, SAdV-27 바이러스 DNA AscI (7951 - 17458) 단편 9507 bp 단편을 클로닝하였다. 정확한 기원을 가지는 클론을 pC5SAD27 PCR Asc로 불렀다.
4. PacI (18409 - 29019) 단편의 삽입.
플라스미드 pC5SAD27 PCR Asc를 Pad로 분해하였고 SAdV-27 바이러스 DNA Pad (18409 - 29019) 10610 bp 단편을 클로닝하였다. 정확한 기원을 가지는 클론을 pC5C27delAsc-Pac로 불렀다.
5. MluI (16026) - SbfI (23007) 단편의 삽입.
플라스미드 pC5C27delAsc-Pac를 MluI + SbfI로 분해하였고 SAdV-27 바이러스 DNA MluI (16026) - SbfI (23007) 6981 bp 단편을 pC5/C27 IP을 만들기 위해 클로닝하였다.
플라스미드 pC5/C27 IP는 E1-결핍 Ad Pan 5 (SAdV-22) 바이러스 DNA를 포함하며, 내부 25603 bp 부분(bp#7955-bp #33557)은 SAdV-27로부터 기능적으로 유사한 24753 bp (bp #7951-bp #32,703) 단편에 의해 대체되었고, 즉, 이것은 말단 전 단백질, 52/55K단백질, 펜톤 염기, pVII, Mu, 헥손, 엔도프로테아제, DNA-결합 단백질 1OOK 스캐폴딩 단백질, 33K 단백질, pVIII, E3 영역, 및 섬유소에 대한 SAdV-22 유전자의 SAdV-27로부터의 그것으로 대체를 초래한다. SAdV-27 단편의 왼쪽 말단에서 AscI 자리는 DNA 폴리머라아제 오픈리딩프레임(1192 잔기 단백질의 잔기 236)의 처음에 있으며, 키메라 단백질을 초래한다. SAdV-27 단편의 오른쪽 말단을 구성하는 EcoRI 자리는 E4 orf 6/7에 대한 오픈리딩프레임 내에 있다. 오른쪽 말단은 Ad Pan 5로부터 PCR 생성된 오른쪽 말단 분획에 연결되어, 재생성된 E4 orf 6/7 번역 생성물은 Ad Pan 5와 SAdV-27 사이의 키메라이다.
B. 표준 분자 생물학 기술을 사용하는 SAdV -28에 기초한 E1-결핍 플라스미드 분자 클론의 구성
두 개의 공개된 기록 및 AdC1을 경험한 발명자가 아군 B 아데노바이러스에서 E1 결핍이 HEK 293 세포에서 Ad5 E1 유전자에 의해 보완되지 않음이 나타냈기 때문에, 하이브리드 아데노바이러스는 AdC1을 사용하는 전략을 기초로 발생되었고[Roy et al., J Virol. Methods. (2007) 141, 14-21 ; Roy et al., J Gen Virol . (2006) 87, 2477-2485], 키메라 구조체의 왼쪽 및 오른쪽 말단은 침팬지 아데노바이러스 Pan 5로부터 유래된다(a.k.a. 유인원 아데노바이러스 22).
출발 플라스미드는 2005년 1월 6일 공개된 WO 2005/001103 A3에서 기술되는 바와 같은 AdC1 키메라 벡터 - pPan5CldelRI의 구성에서 중간체로서 구성되었다.
1. PCR
주형으로서 SAdV-28 바이러스 DNA를 사용하여 pPan5CldelRI (ClaI와 EcoRI 사이)을 PCR-발생 단편으로 대체하였다. PCR 프라이머는 각각 ClaI 및 EcoRI를 함유한다. 프라이머 CCATCTATCGATGCATAATCAGCAAACC [SEQ ID NO: 33, (W33 fwd, Tm - 64.5°)] 및 CTCAAATGGAATTCAAATGTTTAAAG [SEQ ID NO: 34,]를 사용하였다. 프라이머 'W33 fwd'는 ClaI 자리 (밑줄친 곳)를 함유한다. ClaI 자리 다음의 2개의 염기는 야생형 서열에서 CA이지만, ClaI 자리가 박테리아에서 메틸화되지 않는 것으로 변환하기 위해 프라이머에서 GC로 변형되었다. 이 변형은 Ad4 추정 11K 단백질 CR1 델타와 상동인 106개의 아미노산 E3 단백질에서 하나의 아미노산 - S94C -으로 변경된다. 프라이머 'W33 rev'는 EcoRI 자리(밑줄친 곳)를 함유한다. 대응하는 야생형 Ad 서열은 GAATCC이다. 변경된 염기는 이 변경에 의해 바뀌지 않은 이소류신에 대한 코돈(E4 orf 6/7 단백질의 잔기 123)에 있다. ClaI 자리는 SAdV-28의 30273에서 하나와 동일하며; EcoRI 자리는 프라이머에서 만들어져서 Ad Pan 5로부터 E4 영역에서 그것을 스플라이싱하는 것은 키메라 E4 orf 6/7을 만든다(pPan5CldelRI의 EcoRI 자리는 Ad Pan 5 E4 orf 6/7 단백질의 오픈리딩프레임 내이다).
PCR을 NEB Phusion 폴리머라아제를 사용하여 수행하였고, 완충제는 제조업자에 의해 공급되었다.
2474 bp 생성물을 EcoRI 및 ClaI로 분해하여 동일한 두 자리 사이에서 pBluescript II SK+로 클로닝하여 pBS W33 PCR을 수득하였다. 플라스미드 pBS W33 PCR을 ClaI 및 EcoRI 로 분해하였고, 2453 bp PCR-유래(원래) 단편을 동일한 두 자리 사이의 pPan5CldelR1로 클로닝하여 pPan5CldelR1- W33 PCR을 수득하였다.
2. ClaI (18577) 단편( pol 유전자에서 존재하는 AscI 자리와 단지 ClaI 자리 사이)에 AscI (11065)의 삽입.
플라스미드 pPan5CldelR1-W33 PCR을 AscI 및 ClaI로 분해하였고, ClaI (18577) 단편 (pol 유전자에서 존재하는 AscI 자리와 단지 ClaI 자리 사이)에서 SAdV-28 바이러스 DNA AscI (11065)를 클로닝하여, pPan5-W33 delAsc delCla를 수득하였다.
3. AscI (11065) 단편에 AscI ( 7941)의 삽입( 키메라 폴리머라아제 유전자를 만들기 위함).
플라스미드 pPan5-W33 delAsc delCla를 AscI로 분해하였고, AscI (11065)으로 SAdV-28 바이러스 DNA AscI (7941) 단편을 클로닝하였다(키메라 폴리머라아제 유전자를 만든다). 정확한 기원을 가지는 클론을 pPan5-W33 del Cla로 불렀다.
4. ClaI (30273) 단편에 ClaI (18577)의 삽입.
플라스미드 pPan5-W33 del Cla를 ClaI로 분해하였고, ClaI (30273) 단편에서 SAdV-28 바이러스 DNA ClaI (18577)을 클로닝하였다. 정확한 기원을 가지는 클론을 pC5/C28 IP로 불렀다.
플라스미드 pC5/C28 IP는 E1-결핍 Ad Pan 5 (SAdV-22) 바이러스 DNA를 포함하며, 내부 25603 bp 부분(bp#7955-bp #33557)을 SAdV-28로부터 기능적으로 유사한 24779 bp (bp #7946-bp #32657) 부분으로 대체하였고, 즉, 이는 전-말단의 단백질, 52/55K 단백질, 펜톤 염기, pVII, Mu, 헥손, 엔도프로테아제, DNA-결합 단백질, 1OOK 스캐폴딩 단백질, 33K 단백질, pVIII, E3 영역, 및 섬유소에 대한 SAdV-22 유전자를 SAdV-28로부터의 그것들로 대체를 초래한다. SAdV-28 단편의 왼쪽 말단에서 AscI 자리는 DNA 폴리머라아제 오픈리딩프레임(1192 잔기 단백질의 잔기 236)의 시작에 있고, 이는 키메라 단백질을 초래한다. SAdV-28 단편의 오른쪽 말단을 구성하는 EcoRI 자리는 E4 orf 6/7에 대한 오픈리딩프레임 내에 있다. 오른쪽 말단을 Ad Pan 5로부터 PCR 발생 오른쪽 말단 단편에 연결해서, 재발생된 E4 orf 6/7 번역 생성물은 Ad Pan 5 및 SAdV-28 사이의 키메라이다.
C. 표준 분자 생물학 기술을 사용하는 SAdV -29에 기초한 플라스미드 분자 클론의 구성
두 개의 공개된 기록 및 AdC1을 경험한 발명자가 아군 B 아데노바이러스에서 E1 결핍이 HEK 293 세포에서 Ad5 E1 유전자에 의해 보완되지 않음이 나타냈기 때문에, 하이브리드 아데노바이러스는 AdC1을 사용하는 전략을 기초로 발생되었고[Roy et al., J Virol . Methods. (2007) 141, 14-21 ; Roy et al., J Gen Virol . (2006) 87, 2477-2485], 키메라 구조체의 왼쪽 및 오른쪽 말단은 침팬지 아데노바이러스 Pan 5로부터 유래된다(a.k.a. 유인원 아데노바이러스 22).
출발 플라스미드는 2005년 1월 6일 공개된 WO 2005/001103 A3에서 기술되는 바와 같은 AdC1 키메라 벡터 - pPan5CldelRI의 구성에서 중간체와 같이 구성된 것이었다.
1. PCR
주형으로서 SAdV-29 바이러스 DNA를 사용하여 pPan5CldelRI (ClaI 및 EcoRI 사이)로부터의 AdC1 단편을 PCR-발생 단편으로 대체하였다. PCR 프라이머는 각각 ClaI 및 EcoRI 자리를 함유한다. 프라이머 CCATCTATCGATGCATAATCAGCAAACC [SEQ ID NO: 33] & CTCAAATGGAATTCAAATGTTTAAAG [SEQ ID NO: 34]를 사용하였다. 프라이머 'W33 fwd'는 ClaI 자리(밑줄친 곳)를 함유한다. ClaI 자리 다음의 2개의 염기는 야생형 서열에서 CA이지만, ClaI 자리가 박테리아에서 메틸화되지 않는 것으로 변환하기 위해 프라이머에서 GC로 변형되었다. 이 변형은 Ad4 추정 11K 단백질 CR1 델타와 상동인 106개의 아미노산 E3 단백질에서 하나의 아미노산 - S94C -으로 변경된다. 프라이머 'W33 rev'는 EcoRI 자리(밑줄친 곳)를 함유한다. 대응하는 야생형 Ad 서열은 GAATCC이다. 변경된 염기는 이 변경에 의해 바뀌지 않은 이소류신에 대한 코돈(E4 orf 6/7 단백질의 잔기 123)에 있다.
ClaI 자리는 SAdV-29의 30303에서 하나와 동일하며; EcoRI 자리는 프라이머에서 만들어져서 Ad Pan 5로부터 E4 영역에서 그것을 스플라이싱하는 것은 키메라 E4 orf 6/7을 만든다(pPan5CldelR1의 EcoRI 자리는 Ad Pan 5 E4 orf 6/7 단백질의 오픈리딩프레임 내이다).
PCR을 NEB Phusion 폴리머라아제를 사용하여 수행하였고 완충제는 제조업자에 의해 공급되었다.
2480 bp 생성물을 EcoRI 및 ClaI로 분해하여 동일한 두 자리 사이에서 pPan5CldelRI로 클로닝하여 pPan5CldelR1-C29 PCR을 수득하였다.
2. ClaI (18583) 단편( pol 유전자에서 존재하는 AscI 자리와 단지 ClaI 자리 사이)에 AscI (11065)의 삽입.
플라스미드 pPan5C1delR1-C29 PCR을 AscI 및 ClaI으로 분해하였고, ClaI (18583) 단편 (pol 유전자에서 존재하는 AscI 자리와 단지 ClaI 자리 사이)에서 SAdV-28 바이러스 DNA AscI (11065)를 클로닝하여, pPan5-C29 delAsc delCla를 수득하였다.
3. AscI (11094) 단편에 AscI ( 7941)의 삽입( 키메라 폴리머라아제 유전자를 만들기 위함).
플라스미드 pPan5-C29 delAsc delCla를 AscI로 분해하였고, AscI (11094) 단편에서 SAdV-29 바이러스 DNA AscI (7945)를 클로닝하였다(키메라 폴리머라아제 유전자를 만든다). 정확한 기원을 가지는 클론을 pPan5-C29 del Cla로 불렀다.
4. ClaI (30303) 단편에 ClaI (18583)의 삽입.
플라스미드 pPan5-W33 del Cla를 ClaI로 분해하였고, ClaI (30303)에 SAdV-29 바이러스 DNA ClaI (18583) 단편을 클로닝하였다. 정확한 기원을 가지는 클론을 pC5/C29 IP로 불렀다. 플라스미드 pC5/C29 IP는 E1-결핍 Ad Pan 5 (SAdV-22) 바이러스 DNA를 포함하며, 내부 25603 bp 부분(bp#7955-bp #33557)을 SAdV-29로부터 기능적으로 유사한 24817 bp (bp #7945-bp #32761) 부분으로 대체하였고, 즉, 이는 전-말단의 단백질, 52/55K 단백질, 펜톤 염기, pVII, Mu, 헥손, 엔도프로테아제, DNA-결합 단백질, 1OOK 스캐폴딩 단백질, 33K 단백질, pVIII, E3 영역, 및 섬유소에 대한 SAdV-22 유전자를 SAdV-29로부터의 그것들로 대체를 초래한다. SAdV-29 단편의 왼쪽 말단에서 AscI 자리는 DNA 폴리머라아제 오픈리딩프레임(1192 잔기 단백질의 잔기 236)의 시작에 있고, 이는 키메라 단백질을 초래한다. SAdV-29 단편의 오른쪽 말단을 구성하는 EcoRI 자리는 E4 orf 6/7에 대한 오픈리딩프레임 내에 있다. 오른쪽 말단을 Ad Pan 5로부터 PCR 발생 오른쪽 말단 단편에 연결해서, 재발생된 E4 orf 6/7 번역 생성물은 Ad Pan 5 및 SAdV-29 사이의 키메라이다.
D. 표준 분자 생물학 기술을 사용하는 SAdV -32에 기초한 플라스미드 분자 클론의 구성
두 개의 공개된 기록 및 AdC1을 경험한 발명자가 아군 B 아데노바이러스에서 E1 결핍이 HEK 293 세포에서 Ad5 E1 유전자에 의해 보완되지 않음이 나타냈기 때문에, 하이브리드 아데노바이러스는 AdC1을 사용하는 전략을 기초로 발생되었고[Roy et al., J Virol . Methods. (2007) 141, 14-21 ; Roy et al., J Gen Virol . (2006) 87, 2477-2485], 키메라 구조체의 왼쪽 및 오른쪽 말단은 침팬지 아데노바이러스 Pan 5로부터 유래된다(a.k.a. 유인원 아데노바이러스 22).
출발 플라스미드는 2005년 1월 6일 공개된 WO 2005/001103 A3에서 기술되는 바와 같은 AdC1 키메라 벡터 - pPan5C1delRI의 구성에서 중간체와 같이 구성된 것이었다.
1. PCR
pPan5C1delRI (ClaI 및 EcoRI 사이)로부터의 AdC1 부분을 주형으로서 SAdV-32 바이러스 DNA를 사용하여 PCR-발생 단편으로 대체하였다.
프라이머 SEQ ID NO: 202: TTGTAGCATAGTTTGCCTGG[C32 fwd] 및 SEQ ID NO: CTCAAATGGAATTCAAATGTTTAAAG [SEQ ID NO: 34, (W33 rev)]를 사용하였다. 프라이머 'W33 rev'는 EcoRI 자리(밑줄친 곳)를 함유한다.
대응하는 야생형 Ad 서열은 GAATCC이다. 변경된 염기는 이 변경에 의해 바뀌지 않은 E4 orf 6/7 단백질의 이소류신에 대한 코돈에 있다. EcoRI 자리는 프라이머에서 만들어져서 Ad Pan 5로부터 E4 영역에서 그것을 스플라이싱하는 것은 키메라 E4 orf 6/7를 만든다(pPan5CldelRI의 EcoRI 자리는 Ad Pan 5 E4 orf 6/7 단백질의 오픈리딩프레임 내이다).
PCR을 NEB Phusion 폴리머라아제를 사용하여 수행하였고 완충제는 제조업자에 의해 공급되었다.
2259 bp 생성물을 MluI 및 EcoRI로 분해하여 동일한 두 자리 사이에서 pPan5C1delRI로 클로닝하여 pPan5Cl C32 PCR을 수득하였다.
2. SAdV -32의 MluI (16058) 단편에 AscI ( 7945)의 삽입( pol 유전자에서 존재하는 AscI 자리 및 MluI 자리 사이).
플라스미드 pPan5C1 C32 PCR을 AscI 및 MIuI로 분해하였고, Mlul (16058) 단편에서 SAdV-32 바이러스 DNA AscI (7945)를 클로닝하여 pPan5/C32 del Mlu를 수득하였다.
3. MluI (16058 내지 30510) 단편의 삽입.
플라스미드 pPan5/C32 del Mlu를 MIuI로 분해하였고 SAdV-32 바이러스 DNA MluI (16058 내지 30510) 단편을 클로닝하였다. 정확한 기원을 가지는 클론을 pC5/C32 IP로 불렀다.
플라스미드 pC5/C32 IP는 E1-결핍 Ad Pan 5 (SAdV-22) 바이러스 DNA를 포함하며, 내부 25603 bp 부분(bp#7955-bp #33557)을 SAdV-32로부터 기능적으로 유사한 24817 bp (bp #7945-bp #32761) 부분으로 대체하였고, 즉, 이는 전-말단의 단백질, 52/55K단백질, 펜톤 염기, pVII, Mu, 헥손, 엔도프로테아제, DNA-결합 단백질, 1OOK 스캐폴딩 단백질, 33K 단백질, pVIII, E3 영역, 및 섬유소에 대한 SAdV-22 유전자를 SAdV-32로부터의 그것들로 대체를 초래한다. SAdV-32 단편의 왼쪽 말단에서 AscI 자리는 DNA 폴리머라아제 오픈리딩프레임(1192 잔기 단백질의 잔기 236)의 시작에 있고, 이는 키메라 단백질을 초래한다. SAdV-32 단편의 오른쪽 말단을 구성하는 EcoRI 자리는 E4 orf 6/7에 대한 오픈리딩프레임 내에 있다. 오른쪽 말단을 Ad Pan 5로부터 PCR 발생 오른쪽 말단 단편에 연결하여, 재발생된 E4 orf 6/7 번역 생성물은 Ad Pan 5 및 SAdV-32 사이의 키메라이다.
E. 표준 분자 생물학 기술을 사용하는 SAdV -35에 기초한 E1-결핍 플라스미드 분자 클론의 구성
두 개의 공개된 기록 및 AdC1을 경험한 발명자가 아군 B 아데노바이러스에서 E1 결핍이 HEK 293 세포에서 Ad5 E1 유전자에 의해 보완되지 않음이 나타냈기 때문에, 하이브리드 아데노바이러스는 AdC1을 사용하는 전략을 기초로 발생되었고[Roy et al., J Virol. Methods. (2007) 141, 14-21 ; Roy et al., J Gen Virol . (2006) 87, 2477-2485], 키메라 구조체의 왼쪽 및 오른쪽 말단은 침팬지 아데노바이러스 Pan 5로부터 유래된다(a.k.a. 유인원 아데노바이러스 22).
출발 플라스미드는 2005년 1월 6일 공개된 WO 2005/001103 A3에서 기술되는 바와 같은 AdC1 키메라 벡터 - pPan5C1delRI의 구성에서 중간체와 같이 구성된 것이었다.
1. SAdV-35의 EcoRI (22198) 단편으로 AscI (11058)의 삽입.
플라스미드 pPan5C1delRI를 AscI 및 EcoRI로 분해하였고, EcoRI (22198) 단편에서 SAdV-35 바이러스 DNA AscI (11058)를 클로닝하여 pPan5CI C35 Asc-RI를 수득하였다.
2. AscI (7928 내지 11058) 단편의 삽입.
플라스미드 pPan5CI C35 Asc-RI를 AscI로 분해하였고, SAdV-35 바이러스 DNA AscI 단편(7928 내지 11058)을 클로닝하였다. 정확한 방향성(orientation)을 가지는 클론을 pPan5CI C35 Asc-RI + Asc로 불렀다.
3. EcoRI (22198 내지 32738) 단편의 삽입.
플라스미드 pPan5CI C35 Asc-RI + Asc를 EcoRI로 분해하였고, SAdV-35 바이러스 DNA EcoRI 단편(22198 내지 32738)을 클로닝하였다. 정확한 방향성을 가지는 클론을 pC5/C35 IP로 불렀다.
플라스미드 pC5/C35 IP는 E1-결핍 Ad Pan 5 (SAdV-22) 바이러스 DNA를 포함하며, 내부 25603 bp 부분(bp#7955-bp #33557)을 SAdV-35로부터 기능적으로 유사한 24817 bp (bp #7945-bp #32761) 부분으로 대체하였고, 즉, 이는 전-말단의 단백질, 52/55K 단백질, 펜톤 염기, pVII, Mu, 헥손, 엔도프로테아제, DNA-결합 단백질, 1OOK 스캐폴딩 단백질, 33K 단백질, pVIII, E3 영역, 및 섬유소에 대한 SAdV-22 유전자를 SAdV-35로부터의 그것들로 대체를 초래한다. SAdV-35 단편의 왼쪽 말단에서 AscI 자리는 DNA 폴리머라아제 오픈리딩프레임(1192 잔기 단백질의 잔기 236)의 시작에 있고, 이는 키메라 단백질을 초래한다. SAdV-35 단편의 오른쪽 말단을 구성하는 EcoRI 자리는 E4 orf 6/7에 대한 오픈리딩프레임 내에 있다. 오른쪽 말단을 Ad Pan 5로부터 PCR 발생 오른쪽 말단 단편에 연결해서, 재발생된 E4 orf 6/7 번역 생성물은 Ad Pan 5 및 SAdV-35 사이의 키메라이다.
F. E1-결핍 아데노바이러스 벡터
인플루엔자 바이러스 뉴클레오단백질을 발현하는 E1-결핍 아데노바이러스 벡터를 구성하기 위해, H1N1 인플루엔자 A 바이러스 NP를 암호화하는 뉴클레오티드 서열(A/Puerto Rico/8/34/Mount Sinai, GenBank 등록 번호 AF3891 19.1)는 최적화된 코돈이었고 완전히 합성되었다(Celtek Genes, Nashville, TN). 인간 시토메갈로바이러스 초기 프로모터로 구성되는 발현 카세트, 합성 인트론(플라스미드 pCI로부터 획득(Promega, Madison, 위스콘신)), 코돈 최적화 인플루엔자 A NP 코딩 서열 및 소 성장 호르몬 폴리아데닐화 신호를 구성하였다. 플라스미드 pShuttle CMV PI FIuA NP는 상기 기술된 발현 카세트를 포함하며, 이는 각각 희소-절단 제한 효소 I-CeuI 및 PI-SceI (New England Biolabs)에 대한 인식 자리에 의해 측면에 위치된다. E1-결핍 아데노바이러스 벡터의 분자 클론을 만들기 위해, E1-결핍 아데노바이러스의 플라스미드 분자 클론을 우선 만들었고, 희소 절단 제한 효소 I-CeuI 및 PI-Scel에 대한 인식 자리를 E1 결핍 대신 삽입하였다. E1-결핍 아데노바이러스 플라스미드를 그 후 I-CeuI 및 PI-Scel로 분해하였고, 발현 카세트(동일 효소에 의해 분해)를 연결하였다. 결과 아데노바이러스 플라스미드 분자 클론을 HEK 293 세포로 트랜스펙팅하여 재조합 아데노바이러스 벡터를 구제하였다. 트랜스펙션 후 구제를 제한 효소 분해에 의해 플라스미드로부터 선형 아데노바이러스 게놈을 우선 방출함으로써 가능하게 하는 것으로 발견하였다.
실시예 3 - 교차-중화 평가
야생형 SAdV-27, SAdV-28, SAdV-29, SAdV-32, 및 SAdV-35을 직접 면역 형광법에 의해 모니터링되는 감염 억제 중화 항체 분석을 사용하여 인간 아데노바이러스 5(아종 C) 및 침팬지 아데노바이러스 7(SAdV-24), 및 인간 풀링된 IgG와 비교하여 교차-중화 활성에 대해 평가하였다. 일반적 인간 집단이 노출되는 다수의 항원에 대한 항체를 함유하기 때문에, 인간 풀링된 IgG[Hu Pooled IgG]를 상업적으로 구입하고, 면역타협 환자에서 투여를 위해 승인한다. 인간 풀링된 IgG에 대한 유인원 아데노바이러스에서 중화 항체의 존재 또는 부존재는 일반적 모집단에서 이들 아데노바이러스에 대한 항체의 보급의 반영이다.
분석을 하기와 같이 수행하였다. HAdV-5 또는 SAdV-24로 주사한 토끼로부터의 혈청 샘플을 35분 동안 56℃에서 가열하여 불활성화하였다. 야생형 아데노바이러스(108 입자/웰)을 무혈청 둘베코 변형 이글 배지(DMEM)에서 희석하였고, 37℃에서 1시간 동안 DMEM에서 가열-불활성화된 혈청의 2-배 연속 희석으로 배양하였다. 이후에, 혈청-아데노바이러스 혼합물을 105 단일층 A549 세포와 함께 웰 내의 슬라이드에 첨가하였다. 1시간 후, 각 웰의 세포를 100 μl의 20% 소 태아혈청(FBS)-DMEM으로 보충하였고, 5% CO2로 37℃에서 22시간 동안 배양하였다. 다음에, 세포를 PBS로 2회 헹구고 DAPI로 염색하였고, 염소에 FITC로 표지된, 광범위하게 교차 반응성인 항체(Virostat)를 파라포름알데히드(4%, 30 분)에서 고정 및 0.2% Triton (4℃, 20 분)에서 침투 후 HAdV-5에 대해 길렀다. 감염의 수준을 현미경관찰 하에서 FITC 양성 세포의 수를 카운팅함으로써 결정하였다. NAB 타이터를 나이브(naive) 혈청 대조군과 비교하여 50% 이상으로써 아데노바이러스 감염을 억제한 가장 높은 혈청 희석으로서 기록한다.
< 1/20의 타이터 값이 나타나면, 중화 항체 농도는 검출의 제한, 즉 1/20 이하이다.
Figure pat00008
이들 데이터는 일반 모집단에서 SAdV-27, SAdV-29, 및 SAdV-32에 대한 검출가능한 미리 존재하는 면역이 없고; SAdV-28 및 SAdV-35에 대한 최소한의 면역반응성을 나타낸다. 이들 데이터는 HAdV-5 및 SAdV-24와 교차-반응하지 않는 앞의 표에 있는 유인원 아데노바이러스가 아데노바이러스의 순차적 전달을 수반하는 요법, 예를 들어, 프라임-부스트 또는 암 치료법에 유용할 수 있음을 추가로 나타낸다.
실시예 4 - 사이토카인 유도
형질세포양 수지상세포를 인간 말초혈액 단핵구(PBMCs)로부터 분리하였고, 96웰 플레이트로 배지에서 배양하였고 아데노바이러스로 감염시켰다. 48시간 후 세포를 스핀다운하고 상청액을 수집하였고 인터페론 α의 존재하에서 분석하였다.
더 구체적으로, PBMC를 펜실베니아 유니버시티에서 CFAR(Center For AIDS Research) 면역학 코어로부터 획득하였다. 3억개의 이들 세포를 그 후 키트와 함께 제공된 설명서에 따라서 Miltenyi Biotec제의 "인간 형질세포양 수지상세포 분리 키트"를 사용하여 형질세포양 수지상세포(pDCs)를 분리하기 위해 사용하였다. 이 키트를 사용하는 분리는 모든 다른 세포 종류를 제거하는 것을, 그러나 pDC는 PBMC로부터 제거하는 것을 기초로 하였다.
최종 세포 수는 보통 도너로부터 도너까지 다양하지만, 4십만 내지 7십만개의 세포의 범위에 있다. 따라서 발생된 데이터(하기 논의)는 다중 도너로부터의 세포의 분석에서 비롯된다. 그렇지만 놀랍게도, 인터페론 또는 다른 사이토카인 방출에 기초한 아군의 분리는 다양한 도너로부터 세포를 분리할 때조차 유지된다.
세포를 L-글루타민, 10% 소 태아 혈청(Mediatech), 1OmM 헤페스 완충제 용액(Invitrogen), 항생물질(페니실린, 스트렙토마이신 및 겐타마이신-Mediatech 제) 및 인간-인터류킨 3 (20ng/mL - R&D)으로 보충한 RPMI-1640 배지(Mediatech)에서 배양하였다. 야생형 아데노바이러스를 10,000 (세포 당 10,000개의 바이러스 입자, 106 세포/ml의 농도로)의 감염다중도(MOI)에서 세포에 직접 첨가하였다. 48 시간 후, 세포를 스핀 다운하였고, 상청액을 인터페론의 존재하에서 분석하였다. 사이토카인을 제조업자로부터 추천된 프로토콜을 사용하여 PBL 생물의학 연구소로부터 효소-결합면역흡착분석법(ELISA) 키트를 사용하여 분석하였다.
본 연구는 아군 C 아데노바이러스가 IFNα의 검출가능하지 않은 양을 만든다는 것을 나타내었다(본 분석은 1250 pg/mL의 검출 제한을 가진다). 반대로, 아군 E 아데노바이러스의 모든 시험 멤버는 IFNα를 생성하였고, 일반적으로 아군 B 아데노바이러스와 비교하여 상당히 우수한 IFNα를 생성하였다.
다양한 다른 사이토카인을 또한 아데노바이러스의 스크리닝에서 검출하였다. 그러나, 일반적으로, 아군 E 아데노바이러스는 아군 C 아데노바이러스보다 상당히 더 높은 수준의 IL-6, RANTES, MIP-1α, TNF-α, IL-8, 및 IP-10을 생성하였다. 아군 B 아데노바이러스는 또한 IFNα, IL-6, RANTES, 및 MIP1α의 유도에서 아군 C 아데노바이러스를 능가하였다.
상당한 세포 용혈이 이 연구에서 관찰되지 않았기 때문에, 이는 감염과 상관없이, 바이러스 복제의 어떤 상당한 양의 존재하에서 사이토카인이 아군 E 아데노바이러스와 세포를 접촉함으로써 생성된다는 것을 제안한다.
다른 연구에서(제시하지 않음), 세포를 중공 C7 캡시드 단백질(Ad 아군 E) 또는 UV-불활성 아데노바이러스 C7 바이러스 벡터(UV 불활성화는 교차-연결을 야기하며, 아데노바이러스 유전자 발현을 제거한다) 중 하나와 함께 상기 기술된 바와 같이 배양하였다. 이들 연구에서, 동일 또는 더 높은 수준의 IFNα가 무결함 C7과 비교하여 중공 캡시드와 불활성 바이러스 벡터 둘 다에 대해 관찰되었다.
상기 인용된 모든 문헌은 참고로써 본원에 포함된다. 수많은 변형 및 변경이 상기 확인된 설명의 범주에 포함되며 당업자에게 명백한 것으로 기대된다. 이러한 조성물 및 공정에 대한 변형 및 변경, 예로써 다른 미니유전자의 선택 또는 벡터 또는 면역 조절자의 선택 또는 투약량은 첨부되는 청구항의 범주 내인 것으로 믿어진다.
SEQUENCE LISTING <110> The Trustees of the University of Pennsylvania Roy, Soumitra Wilson, James Vandenberghe, Luc <120> Simian Subfamily B Adenoviruses SAdV-28, -27, -29, -32, -33, and -35 and Uses Thereof <130> UPN-U4611PCT <150> US 61/004,531 <151> 2007-11-28 <150> US 61/004,567 <151> 2007-11-28 <150> US 61/004,534 <151> 2007-11-28 <150> US 61/004,466 <151> 2007-11-28 <150> US 61/004,542 <151> 2007-11-28 <150> US 61/004,533 <151> 2007-11-28 <160> 202 <170> PatentIn version 3.5 <210> 1 <211> 35610 <212> DNA <213> Simian adenovirus 28 <220> <221> repeat_region <222> (1)..(132) <223> label=ITR <220> <221> CDS <222> (1917)..(3401) <223> label=Elb\55K <220> <221> CDS <222> (3496)..(3909) <223> label=pIX <220> <221> misc_feature <222> (3978)..(8899) <223> complement (3978..5308, 5587..5599) label=IVa2 <220> <221> misc_feature <222> (5081)..(13890) <223> complement (5081..8653, 13882..13890) label=pol <220> <221> misc_feature <222> (8455)..(13890) <223> complement (8455..10410, 13882..13890) label=pTP <220> <221> CDS <222> (10893)..(12059) <223> label=52K <220> <221> CDS <222> (12087)..(13847) <223> label=pIIIa <220> <221> CDS <222> (13935)..(15680) <223> label=penton <220> <221> CDS <222> (15692)..(16267) <223> label=pVII <220> <221> CDS <222> (16313)..(17362) <223> label=V <220> <221> CDS <222> (17394)..(17618) <223> label=pX <220> <221> CDS <222> (17696)..(18445) <223> label=pVI <220> <221> CDS <222> (18564)..(21395) <223> label=hexon <220> <221> CDS <222> (21430)..(22056) <223> label=protease <220> <221> misc_feature <222> (22152)..(23708) <223> complement label=DBP <220> <221> CDS <222> (23739)..(26222) <223> label=100K <220> <221> CDS <222> (26882)..(27562) <223> label=pVIII <220> <221> CDS <222> (27565)..(27879) <223> label=E3\12.5K <220> <221> CDS <222> (28267)..(28800) <223> label=E3\gp19K <220> <221> CDS <222> (29415)..(29978) <223> label=E3\CR1-gamma <220> <221> CDS <222> (29997)..(30314) <223> label=E3\CR1-delta <220> <221> CDS <222> (30347)..(30619) <223> label=E3\RID-alpha <220> <221> CDS <222> (31006)..(31401) <223> label=E3\14.7K <220> <221> CDS <222> (31630)..(32598) <223> label=fiber <220> <221> misc_feature <222> (32642)..(33816) <223> complement (32642..32890, 33613..33816) label= E4\orf6/7 <220> <221> misc_feature <222> (32890)..(33816) <223> complement label=E4\orf6 <220> <221> misc_feature <222> (33692)..(34072) <223> complement label=E4\orf4 <220> <221> misc_feature <222> (34085)..(34435) <223> complement label=E4\orf3 <220> <221> misc_feature <222> (34435)..(34821) <223> complement label=E4\orf2 <220> <221> misc_feature <222> (34862)..(35233) <223> complement label=E4\orf1 <220> <221> repeat_region <222> (35479)..(35610) <223> complement label=ITR <400> 1 catcatcaat aatatacctt ataaatggaa cggtgccaac atgcaaatga gcttttgaaa 60 atggagggcg gaaggggatt ggccagcggg ttcaacggtc aaaaggggcg ggccggcgcg 120 gggaggtgac gtatttcgtg tgggaggagt tttgttgcaa gttatcgcgg caaaagtgac 180 gtaaaacgag gtgtggtttg aacacggaag tagacagttt tcccgcgctg actgacagga 240 tatgaggtag ttttgggcgg atgcaagtga aaattcacca ttttcgcgcg aaaactgaat 300 gaggaagtga atttctgagt aatttcgagt ttatgacagg gtggagtatt taccgagggc 360 cgagtagact ttgaccgatt acgtggaggt ttcgattacc gtgtttttca cctaaatttc 420 cgcgtacggt gtcaaagtcc tgtgttttta cgtaggcgtc agctgatcgc tagggtattt 480 aaacctgacg agttccgtca agaggccact cttgagtgcc agcgagaaga gatttctcct 540 ccgcgccgcg agtcagatct ccactttgaa aaaatgagac acctgcgatt cctgcctcag 600 gaaatctcca ttgagaccgg gaatgaaata ctacagcttg tggtaaatgc cctgatggga 660 gacgatccgg agccgcctgc gcatccgttc gatcctccta cgcttcatga actgtatgat 720 ttagaggtag atgggccgga ggatcctaac gaggaagctg tgaatggttt ttttagcgaa 780 tctatgctat tggctgctaa tgaaggagtg gacatagacc caccttctga gacccttgat 840 accccagggg tgattgtgga gagcggcaga ggtgggaaaa aattgcctga acttggtgct 900 gctgaaatgg acttgcactg ttatgaagag ggttttcctc cgagtgatga agaggaggaa 960 aatgtgcagt cgatccagac cgcagcgggt gagggaatga aagctgccaa tgatggtttt 1020 aagttggact gcccggagct gcctggacat ggctgtaagt cttgtgaatt tcacaggaat 1080 agtactggac taaaagaact gttgtgctcg ctttgctata tgagaacgca ctgccatttt 1140 atttacagta agtgtgttta acttaaattt aaagggacag tgtagcagtt taatgtctgt 1200 tgaatgtggg atttatgtct ttgtgatttt tataggtcct gtgtcagatg ctgatgaatc 1260 gccttctcct gattcaacta cctcacctcc tgaaattcag gcgccagtcc ctgcaaacgt 1320 atgcaagccc attcctgtga aggctaagcc tgggaaacgc cctgctgtgg ataagctgga 1380 ggacttgctt gagggtgggg atggaccttt ggacttgagt acccggaaac tgccaaggca 1440 atgagtgccc tgcacctgtg tttatttaat gtgacgtcag tatttatgtg agagtgccat 1500 gtaataaaat tatgtcagct gctgagtatt ttattgcttc ttgggtgggg acttggatat 1560 ataagtagga gcagacctgt gtggttagct cacagcagct tgctgccatc catggaggtt 1620 tgggctatct tggaagatct caggcagact aggcaactgc tagaaaacgc ctcggacgga 1680 gtctctagtc tttggagatt ctggttcggt ggtgatctag ctaggctagt ctttagggta 1740 aaacgggagt atagtgaaga atttgaaaag ttattggaag acagtccagg actttttgaa 1800 gcccttaact tgggccacca ggctcatttt aaggagaagg ttttatcagt tttagatttt 1860 tctacccctg gtagaactgc tgctgctgta gctttcctta cttttatatt ggataa atg 1919 Met 1 gat ccc aca aac cca ctt cag caa ggg ata cgt ctt gga ttt cat agc 1967 Asp Pro Thr Asn Pro Leu Gln Gln Gly Ile Arg Leu Gly Phe His Ser 5 10 15 agc agc ttt gtg gag aac atg gaa ggc ccg cag gct gag gat aat ctt 2015 Ser Ser Phe Val Glu Asn Met Glu Gly Pro Gln Ala Glu Asp Asn Leu 20 25 30 aga tta ctg gcc agt gca gcc tct ggg cgt agc ggc gat cct gag aca 2063 Arg Leu Leu Ala Ser Ala Ala Ser Gly Arg Ser Gly Asp Pro Glu Thr 35 40 45 ccc acc ggc cat gcc agc ggt ttt gga gga gga gca gca gga gga caa 2111 Pro Thr Gly His Ala Ser Gly Phe Gly Gly Gly Ala Ala Gly Gly Gln 50 55 60 65 ccc gag agc cgg cct gga ccc tcc ggt gga gga ggc gga gga gta gct 2159 Pro Glu Ser Arg Pro Gly Pro Ser Gly Gly Gly Gly Gly Gly Val Ala 70 75 80 gac ctg ttt cct gaa ctg cga cgg gtg ctt act agg tct acg tcc agt 2207 Asp Leu Phe Pro Glu Leu Arg Arg Val Leu Thr Arg Ser Thr Ser Ser 85 90 95 gga cag gac agg ggc att aag agg gag agg aat gct agt ggt cat aat 2255 Gly Gln Asp Arg Gly Ile Lys Arg Glu Arg Asn Ala Ser Gly His Asn 100 105 110 tca aga act gag ttg gct tta agt tta atg agt cgc agc cgc cct gaa 2303 Ser Arg Thr Glu Leu Ala Leu Ser Leu Met Ser Arg Ser Arg Pro Glu 115 120 125 act atc tgg tgg cat gag gtt cag agc gag ggc agg gat gaa gtt tca 2351 Thr Ile Trp Trp His Glu Val Gln Ser Glu Gly Arg Asp Glu Val Ser 130 135 140 145 ata ttg cag gag aaa tat tct cta gaa caa att aaa acc tgt tgg ttg 2399 Ile Leu Gln Glu Lys Tyr Ser Leu Glu Gln Ile Lys Thr Cys Trp Leu 150 155 160 gaa cct gag gat gat tgg gag gtg gcc att agg aat tat gct aag ata 2447 Glu Pro Glu Asp Asp Trp Glu Val Ala Ile Arg Asn Tyr Ala Lys Ile 165 170 175 tct ctg agg cct gat aag cag tat aga att acc aag aag att aat atc 2495 Ser Leu Arg Pro Asp Lys Gln Tyr Arg Ile Thr Lys Lys Ile Asn Ile 180 185 190 aga aat gca tgc tac ata tca ggg aat ggg gca gag gtt ata ata gat 2543 Arg Asn Ala Cys Tyr Ile Ser Gly Asn Gly Ala Glu Val Ile Ile Asp 195 200 205 aca cca gat aaa aca gct ttt agg tgt tgt atg atg ggt atg tgg cca 2591 Thr Pro Asp Lys Thr Ala Phe Arg Cys Cys Met Met Gly Met Trp Pro 210 215 220 225 ggg gtg gct ggt atg gag gca gta acc ctt atg aat ata agg ttt agg 2639 Gly Val Ala Gly Met Glu Ala Val Thr Leu Met Asn Ile Arg Phe Arg 230 235 240 gga gat ggg tat aat ggg att gtc ttt atg gct aac act aaa tta att 2687 Gly Asp Gly Tyr Asn Gly Ile Val Phe Met Ala Asn Thr Lys Leu Ile 245 250 255 ctg cac ggt tgt agc ttt ttt ggg ttt aat aat act tgt gtg gaa gct 2735 Leu His Gly Cys Ser Phe Phe Gly Phe Asn Asn Thr Cys Val Glu Ala 260 265 270 tgg ggg cag gtc agt gta aga ggc tgt agt ttt tat gca tgc tgg att 2783 Trp Gly Gln Val Ser Val Arg Gly Cys Ser Phe Tyr Ala Cys Trp Ile 275 280 285 gca cta tca ggc agg acc aaa agt cag ttg tct gtg aag aaa tgc atg 2831 Ala Leu Ser Gly Arg Thr Lys Ser Gln Leu Ser Val Lys Lys Cys Met 290 295 300 305 ttt gag aga tgt aac ctg ggc ata ctg aat gaa ggt gaa gca agg gtc 2879 Phe Glu Arg Cys Asn Leu Gly Ile Leu Asn Glu Gly Glu Ala Arg Val 310 315 320 cgc cac tgc gct gct aca gaa act ggc tgc ttc att cta ata aag gga 2927 Arg His Cys Ala Ala Thr Glu Thr Gly Cys Phe Ile Leu Ile Lys Gly 325 330 335 aat gcc agt gtg aag cat aac atg atc tgt gga ccc tcg gat gag agg 2975 Asn Ala Ser Val Lys His Asn Met Ile Cys Gly Pro Ser Asp Glu Arg 340 345 350 cct tat cag atg ctg acc tgt gct gga gga cat tgc aat atg ctg gct 3023 Pro Tyr Gln Met Leu Thr Cys Ala Gly Gly His Cys Asn Met Leu Ala 355 360 365 act gtg cat att gtt tct cat gca cgc aag aaa tgg cct gtg ttt gag 3071 Thr Val His Ile Val Ser His Ala Arg Lys Lys Trp Pro Val Phe Glu 370 375 380 385 cat aat gtg atg acc aag tgc acc atg cac ata ggt ggt cgc agg gga 3119 His Asn Val Met Thr Lys Cys Thr Met His Ile Gly Gly Arg Arg Gly 390 395 400 atg ttt atg cct tac cag tgt aac atg aat cat gtg aag gtg atg ttg 3167 Met Phe Met Pro Tyr Gln Cys Asn Met Asn His Val Lys Val Met Leu 405 410 415 gaa cca gat gcc ttt tcc aga atg agc tta aca gga atc ttt gat atg 3215 Glu Pro Asp Ala Phe Ser Arg Met Ser Leu Thr Gly Ile Phe Asp Met 420 425 430 aat gtg caa cta tgg aag atc ctg aga tat gat gag acc aaa tcg agg 3263 Asn Val Gln Leu Trp Lys Ile Leu Arg Tyr Asp Glu Thr Lys Ser Arg 435 440 445 gtg cgc gca tgc gaa tgc ggg ggc aag cat gcc agg ttc cag ccg gtg 3311 Val Arg Ala Cys Glu Cys Gly Gly Lys His Ala Arg Phe Gln Pro Val 450 455 460 465 tgt gtg gat gtg acg gaa gac ctg aga ccc gat cat ttg gtg ctt gcc 3359 Cys Val Asp Val Thr Glu Asp Leu Arg Pro Asp His Leu Val Leu Ala 470 475 480 tgc act gga gcg gag ttc ggt tct agt ggg gaa gaa act gac 3401 Cys Thr Gly Ala Glu Phe Gly Ser Ser Gly Glu Glu Thr Asp 485 490 495 taaagtgagt agtggggaat gctttggagg ggattccagg cgggtaaggt gggcagattg 3461 ggtaaattct gtttgtttct gtcttgcagc tgcc atg agt gga agc gct tct ttt 3516 Met Ser Gly Ser Ala Ser Phe 500 gag ggg gga gtc ttt agc cct tat ctg acg ggg cga ctc cca ccc tgg 3564 Glu Gly Gly Val Phe Ser Pro Tyr Leu Thr Gly Arg Leu Pro Pro Trp 505 510 515 gca gga gtt cgt cag aat gtc atg gga tcc act gtg gat ggg aga ccc 3612 Ala Gly Val Arg Gln Asn Val Met Gly Ser Thr Val Asp Gly Arg Pro 520 525 530 gtc cag ccc gcc aat tcc tca acg ctg acc tat gcc act ttg agc tct 3660 Val Gln Pro Ala Asn Ser Ser Thr Leu Thr Tyr Ala Thr Leu Ser Ser 535 540 545 550 tca tcc ttg gat gca gcc gca gcc gct gcc gcc tct gct gcc gcc aac 3708 Ser Ser Leu Asp Ala Ala Ala Ala Ala Ala Ala Ser Ala Ala Ala Asn 555 560 565 act gtc ctt gga atg ggc tat tat gga agc atc gtt gcc aat tcc agt 3756 Thr Val Leu Gly Met Gly Tyr Tyr Gly Ser Ile Val Ala Asn Ser Ser 570 575 580 tcc tct aat aac cct tcg acc ctg gct gag gac aag cta ctt gtc ctc 3804 Ser Ser Asn Asn Pro Ser Thr Leu Ala Glu Asp Lys Leu Leu Val Leu 585 590 595 ttg gct cag ctc gag gca ttg acc cag cgc cta ggc gaa ctg tct cag 3852 Leu Ala Gln Leu Glu Ala Leu Thr Gln Arg Leu Gly Glu Leu Ser Gln 600 605 610 cag gtg gcc cag ttg cgc gag caa act gag tct gct gtt gcc aca gca 3900 Gln Val Ala Gln Leu Arg Glu Gln Thr Glu Ser Ala Val Ala Thr Ala 615 620 625 630 aag tct aaa taaagattcc caaatcaata aataaaggag atccttgttg 3949 Lys Ser Lys attgtaaaac aagtgtaatg aatctttatt tgatttttcg cgcgcggtat gccctggacc 4009 accggtctcg atcattgaga actcggtgga tcttttccag gaccctgtag aggtgggatt 4069 gaatgtttag atacatgggc attaggccgt ctcgggggtg gagatagctc cattgaagag 4129 cctcatgctc cggggtagtg ttataaatca cccagtcata acaaggtcgg agtgcatggt 4189 gttgcacaat atcttttagg agcaggctaa ttgcaacggg gaggccctta gtgtaggtgt 4249 ttacaaatct gttgagctgg gacgggtgca tccggggtga aattatatgc attttggact 4309 ggatcttgag gttggcaatg ttgccgccta gatcccgtct cgggttcata ttgtgcagga 4369 ccaccaagac agtgtatccg gtgcacttgg gaaatttatc atgcagctta gagggaaaag 4429 catgaaaaaa tttggagacg cctttgtgtc cgcccagatt ctccatgcac tcatccataa 4489 tgatagcgat gggaccgtgg gcggcggcgc gggcaaacac gttccggggg tctgacacat 4549 catagttatg ctcctgagac aggtcatcat aagccatttt aataaacttg gggcggaggg 4609 tgccagattg ggggatgaaa gttccctcgg gccccggagc atagtttccc tcacatattt 4669 gcatttccca agctttcagt tcagaggggg ggatcatgtc cacctgcggg gctataaaaa 4729 ataccgtttc tggagccggg gtgattaact gggatgagag cagattcctg agcagctgag 4789 acttgccgca cccagtggga ccgtaaatga ccccgattac gggttgcaga tggtagttta 4849 gggagcggca gctgccgtcc tcccggagca ggggggccac ttcgttcatc atttccctta 4909 catggatatt ttcccgcacc aagtccgtta ggaggcgctc tccccccagg gatagaagct 4969 cctggagcga ggagaagttt ttcagcggct tcagcccgtc agccatgggc attttggaga 5029 gagtctgttg caagagctct agtcggtccc agagctcggt gatgtgttct atggcatctc 5089 gatccagcag acctcctcgt ttcgcgggtt gggacgactc ctggagtatg gtatcagacg 5149 atgggcgtcc agcgctgcca gggtccgatc tttccagggt cgcagcgtcc gagtcagggt 5209 tgtttccgtc acggtgaagg ggtgcgcgcc tggttgggcg cttgcgaggg tgcgtttcag 5269 gctcatcctg ctggtcgaga accgctgccg atcggcgccc tgcatgtcgg ccaggtagca 5329 gtttaccatg agttcgtagt tgagcgcctc ggccgcgtgg cctttggcgc ggagcttacc 5389 tttggaagtt ttctggcagg cggggcagta cagacacttg agggcataca gtttgggagc 5449 gaggaagatg gattcggggg agtatgcgtc cgcaccgcag gaggcgcaga cggtttcgca 5509 ttccacgagc caggtcagat ccggctcatc ggggtcaaaa acaagttttc ccccatgttt 5569 tttgatgcgt ttcttacctt tggtctccat gagttcgtgt ccccgctggg tgacaaagag 5629 gctgtccgtg tccccgtaga ccgattttat gggcctgtcc tcgagcggag tgcctcggtc 5689 ctcttcgtag aggaactcgg accactctga tacaaaggcg cgcgtccagg ccagcacaaa 5749 agaggccacg tgggaggggt agcggtcgtt gtcaaccagg gggtccacct tctccacggt 5809 atgtaaacac atgtccccct cctccacatc caagaatgtg attggcttgt aagtgtatgc 5869 cacgtgacca ggggtccccg ccgggggggt ataaaagggg gcgggtctct gctcgtcctc 5929 actgtcttcc ggatcgctgt ccaggagcgc cagctgttgg ggtaggtatt ccctctcaaa 5989 ggcgggcata acctctgcac tcaggttgtc agtttctagg aacgaggagg atttgatatt 6049 gacagtgcca gccgagatgc ctttcataag actctcgtcc atttggtcag aaaatacaat 6109 ctttttgttg tccagcttgg tggcaaagga tccatagagg gcattggata agagcttggc 6169 tatggagcgc atggtttggt tcttttcctt gtcagcgcgc tccttggcag caatgttgag 6229 ctggacatac tcgcgcgcca gacacttcca ttcagggaag atggttgtca gttcatctgg 6289 cacgattctg actcgccagc ccctgttatg cagggtgatc agatccacac tggtggtcac 6349 ttcgcctctg aggggctcgt tggtccagca gagtcgaccc ccttttctcg aacagaaagg 6409 tgggaggggg tctagcatga gttcatcagg ggggtctgca tccatagtga agattcctgg 6469 gagcagatcc ttgtcaaaat agctgatggg tgtggggtca tccaaagcca tctgccattc 6529 tcgagctgcc agcgcgcgct cataggggtt gagaggggtg ccccatggca tggggtgggt 6589 gagtgcagag gcatacatgc cacagatgtc atagacatag aggggctctt cgaggatgcc 6649 aatgtaggtg ggataacagc gcccccctct gatgcttgct cgcacatagt catagagttc 6709 atgcgagggg gcgagcagac ccgagcccaa attagtgcga ttgggttttt cagccctgta 6769 gacgatctgg cgaaagatgg catgtgaatt tgaagagatg gtgggtctct gaaagatgtt 6829 aaaatgggca tgaggtagac ctacagagtc cctgatgaag tgggcatatg actcttgcag 6889 cttggccacc agctctgcag tgacaaggac atccagggcg cagtagtcaa gggtctcttg 6949 gatgatgtca taacctagtt ggtttttttt ttcccacagc tcgcggttga gaaggtattc 7009 ttcgcgatcc ttccagtact cttcgagggg aaacccgtct ttgtctgcac ggtaagagcc 7069 cagcatgtag aactgattaa ctgctttgta gggacagcat cccttctcca cggggagaga 7129 gtatgcttgg gctgccttgc gcagtgaggt atgagtgagg gcgaaggtgt ccctgaccat 7189 gactttgagg aactggtact tgaaatcgat gtcatcacag gccccctgtt cccagagttg 7249 gaagtccacc cgcttcttgt aggcggggtt gggcaaagcg aaagtaacat cattgaagag 7309 aatcttgccg gccctgggca tgaaattgcg ggtgatgcgg aaaggctggg gcacctctgc 7369 ccggttattg atgacctgag cggctaggac gatctcgtca aagccattga tgttgtgtcc 7429 cacaatgtaa agttctatga atcgcggggt gcccctgaca tgaggcagct tcttgagttc 7489 ttcaaaagtg aggtctgtag ggtcagatag agcatagtgt tcgagggccc attcgtgcag 7549 gtgagggttt gcattgagga aggaggacca gagatccact gccagtgctg tttgtaactg 7609 gtctcggtac tggcgaaaat gctggccgac tgccatcttt tctggggtga tacagtagaa 7669 ggttttgggg tcttgctgcc agcgatccca cttgagtttc atggcgaggt cgtaggcgat 7729 gttgacgagc cgctcgtccc ccgaaagttt catgaccagc atgaagggga ttagctgctt 7789 gccaaaggac cccatccagg tgtaggtttc cacatcgtag gtgaggaaga gcctttctgt 7849 gcgaggatga gagccgatcg ggaagaactg gatctcctgc caccagttgg aggaatggct 7909 gttgatgtga tggaagtaga actccctgcg gcgcgccgag cattcatgct tgtgcttata 7969 cagacggccg cagtactcgc agcgcttcac gggatgcacc tcatgaatga gttgtacctg 8029 gcttcctttg acgagaaatt tcagtgggaa gttgaggcct ggcgcttgta cctcgcgctc 8089 tactatgtta tctgcatcgg cctggccatc ttctgtctcg atggtggtca tgctaacgag 8149 cccccgcggg aggcaagtcc agacctcggc gcgggagggg cggagctcga ggacgagagc 8209 gcgcaggccg gagctgtcca gggtcctgag tcgctgcgga gtcaggttag taggtagcgt 8269 caggagatta acttgcatga tcttttcgag ggcatgcggg aggttcagat ggtacttgat 8329 ctccacgggt ccgttggagg agatgtcgat ggcttgcagg gtcccgtgcc ccttgggcgc 8389 caccaccgtg cccttgtttt tccttttggg cggaggaggc ggctctgttg cttcttgcat 8449 gttcagaagc ggtggcgagg gcgcgcgccg ggcggtaggg gcggctctgg ccccggcggc 8509 atggctggca gaggcacgtc ggcgccgcgc gcgggtaggt tctggtactg cgccctgaga 8569 agacttgcgt gcgcgacgac gcggcggttg acgtcctgga tctgacgcct ctgggtgaaa 8629 gctaccggac ccgtgagctt gaacctgaaa gagagttcaa cagaatcaat ttcggtatcg 8689 ttgacggcgg cttgcctcag gatctcttgc acgtcgcccg agttgtcctg gtaggcgatc 8749 tcggccatga actgctcgat ttcttcctcc tgaagatctc cgcggcccgc tctctcgacg 8809 gtggcagcaa ggtcgttgga gatgcgaccc atgagttgag agaatgcatt catgcccgcc 8869 tcgttccaga cgcggctgta gaccacggcc ccctcgggat ctctcgcgcg catgaccacc 8929 tgggcgaggt tgagctccac gtggcgggtg aagaccgcat agttgcatag gcgctggaag 8989 aggtagttga gtgtggtggc gatgtgctcg gtgacgaaga aatacatgat ccatcgtctc 9049 agcggcattt cgctgacatc gcccagggct tccaagcgct ccatggcctc gtagaagtcc 9109 acagcgaagt tgaaaaactg ggagttgcgc gcggacacgg tcaactcctc ctccagaaga 9169 cggatgagat cggcgatggt ggcgcgcacc tcgcgctcga aagcccccgg gatttcttcc 9229 tcctcctctt ctatctcttc ttccactaac atctcttctt cctcttcagg cgggggcgga 9289 ggaggagggg gcgcgcggcg acgccggcgg cgcacgggca gacggtcgat gaatctttca 9349 atgacctctc cgcggcggcg gcgcatggtc tcggtgacgg cgcggccgtt ctccctgggt 9409 ctcagagtga agacgcctcc gcgcatctcc ctgaagtggt gactgggtgg ctctccgttg 9469 ggcagggaca gggcgctgat gatgcatttt atcaattgtc ccgtagggac tccgcgcaag 9529 gacctgatcg tctgaagatc cacgggatct gaaaaccttt cgacgaaagc gtctaaccag 9589 tcgcaatcgc aaggtaggct gagcaccgtt tcttgcgggc gggggttctc tcttccttct 9649 ccttcctcat catctcggga gggtgagacg atgctgctgg tgatgaaatt aaaataggca 9709 gttctgagac ggcggatggt ggcgaggagc accaggtctt tgggtccggc ttgctggatg 9769 cgcaggcgat cggccattcc ccaagcattg tcctggcatc tggccagatc tttatagtag 9829 tcttgcatga gtcgctccac gggcacttct tcttcgcccg ctctgccatg catgcgcgtg 9889 agtccgaacc cgcgcatggg ctggacaagt gccaggtccg ctacgaccct ttcggcgagg 9949 atggcttgct gcacctgggt gagggtggct tggaagtcgt caaagtccac gaagcgatgg 10009 taggccccgg tgttgatggt gtaggagcag ttggccatga ctgaccagtt gactgtctgg 10069 tgccccgggc gcacgagctc ggtgtacttg agtcgcgagt aggcgcgggt atcaaagatg 10129 taatcgttgc aggtgcgcac caggtactgg tagccgatga gaaagtgcgg cggtggctgg 10189 cggtagaggg gccatcgctc tgtagccggg gctccggggg cgaggtcttc cagcatgagg 10249 cggtggtatc cgtagatgta cctggacatc caggtgatcc cggaggcggt ggtggacgcc 10309 cgagggaact cgcgcactcg gttccagatg ttgcgcagcg gcatgaagta gttcatggta 10369 ggcacggtct ggccagtaag gcgggcgcag tcattgatgc tctatagaca cggagaaaac 10429 gaaagcgatg agcggctcgc ctccgtggcc tggaggaacg tgaacgggtt gggtcgcggt 10489 gtaccccggt tcgagacaca agccaagcga gcacaactcg ggccggccgg agccgtggct 10549 aacgtggtat tggcgatccc gtctcgaccc agccgacgaa tatccaggat acggagtcga 10609 gtcgttttgc tgcttgttgc tttttcctgg acgggagcca gtgccgcgtc aagctttaga 10669 acgctcagtt cacggggccg ggagtggctc gcgcccgtag tctggagaat caatcgccag 10729 ggttgcgttg cggtgtgccc cggttcgagt cttagcgtgg cccggatcgg ccggtttccg 10789 cggcaagcga gggtttggca gccccgtcat ttctaagacc ccgccagccg acttctccag 10849 tttacgggag cgagccctct ttttttttgt ttttgtcgcc cag atg cat ccc gtg 10904 Met His Pro Val 635 ctg cga cag atg cgc ccc cag caa cag gtc cct tct cag caa cag cag 10952 Leu Arg Gln Met Arg Pro Gln Gln Gln Val Pro Ser Gln Gln Gln Gln 640 645 650 cag cca caa aag gct ctt cct gct cct gct cct gca act act gca gtc 11000 Gln Pro Gln Lys Ala Leu Pro Ala Pro Ala Pro Ala Thr Thr Ala Val 655 660 665 gca gcc gtg tgc ggc gcg gga cag ccc gcc tat gat ctg gac ttg gaa 11048 Ala Ala Val Cys Gly Ala Gly Gln Pro Ala Tyr Asp Leu Asp Leu Glu 670 675 680 685 gag ggc gag gga ctg gcg cgc ctg ggt gca cca tcg ccc gag cga cac 11096 Glu Gly Glu Gly Leu Ala Arg Leu Gly Ala Pro Ser Pro Glu Arg His 690 695 700 ccg cgg gtg caa ctg aaa aag gac tct cgc gag gca tac gtg ccc cag 11144 Pro Arg Val Gln Leu Lys Lys Asp Ser Arg Glu Ala Tyr Val Pro Gln 705 710 715 cat aac ctg ttc agg gac agg agc ggc gag gag ccc gag gag atg cga 11192 His Asn Leu Phe Arg Asp Arg Ser Gly Glu Glu Pro Glu Glu Met Arg 720 725 730 gcc tcc cgc ttt aac gcg ggt cgc gag ctg cgc cac ggt ctg gac cga 11240 Ala Ser Arg Phe Asn Ala Gly Arg Glu Leu Arg His Gly Leu Asp Arg 735 740 745 aga cgg gtg ctg cgg gac gag gat ttc gag gtc gat gaa gtg aca ggg 11288 Arg Arg Val Leu Arg Asp Glu Asp Phe Glu Val Asp Glu Val Thr Gly 750 755 760 765 atc agc ccc gct agg gca cat gtg gcc gcg gcc aac ctg gtc tcg gcc 11336 Ile Ser Pro Ala Arg Ala His Val Ala Ala Ala Asn Leu Val Ser Ala 770 775 780 tac gag cag acc gtg aag gag gag cgc aac ttc caa aaa tca ttt aac 11384 Tyr Glu Gln Thr Val Lys Glu Glu Arg Asn Phe Gln Lys Ser Phe Asn 785 790 795 aac cat gtg cgc acc ctg atc gcc cgt gag gaa gtg act ctg ggt ctg 11432 Asn His Val Arg Thr Leu Ile Ala Arg Glu Glu Val Thr Leu Gly Leu 800 805 810 atg cac ctg tgg gac ctg atg gaa gct atc acc cag aac ccc act agc 11480 Met His Leu Trp Asp Leu Met Glu Ala Ile Thr Gln Asn Pro Thr Ser 815 820 825 aaa ccc ctg acc gct cag ctg ttt ctg gta gtg caa cat agc agg gac 11528 Lys Pro Leu Thr Ala Gln Leu Phe Leu Val Val Gln His Ser Arg Asp 830 835 840 845 aat gag gca ttc agg gag gcg ctg ctg aac atc acc gag ccc gag ggg 11576 Asn Glu Ala Phe Arg Glu Ala Leu Leu Asn Ile Thr Glu Pro Glu Gly 850 855 860 aga tgg ttg tat gat ctg atc aat atc ctg cag agt att ata gtg cag 11624 Arg Trp Leu Tyr Asp Leu Ile Asn Ile Leu Gln Ser Ile Ile Val Gln 865 870 875 gaa cgt agc ctg ggt ctg gct gag aaa gtg gca gcc atc aac tac tcg 11672 Glu Arg Ser Leu Gly Leu Ala Glu Lys Val Ala Ala Ile Asn Tyr Ser 880 885 890 gtc ttg agc ctg ggc aag tac tac gct cgc aag atc tac aag acc ccc 11720 Val Leu Ser Leu Gly Lys Tyr Tyr Ala Arg Lys Ile Tyr Lys Thr Pro 895 900 905 tac gtg ccc ata gac aag gag gtg aag ata gat ggg ttt tac atg cgc 11768 Tyr Val Pro Ile Asp Lys Glu Val Lys Ile Asp Gly Phe Tyr Met Arg 910 915 920 925 atg act ctt aaa gtg ctg act ctc agc gac gat ttg ggg gtg tac cgc 11816 Met Thr Leu Lys Val Leu Thr Leu Ser Asp Asp Leu Gly Val Tyr Arg 930 935 940 aac gac agg atg cac cgc gcg gtg agc gcc agc agg agg cgc gag ctg 11864 Asn Asp Arg Met His Arg Ala Val Ser Ala Ser Arg Arg Arg Glu Leu 945 950 955 agc gac aga gaa ctt atg cac agc ttg caa aga gct ctg acg ggg gca 11912 Ser Asp Arg Glu Leu Met His Ser Leu Gln Arg Ala Leu Thr Gly Ala 960 965 970 ggg acc gat ggg gag aac tac ttt gac atg ggg gca gac ttg cag tgg 11960 Gly Thr Asp Gly Glu Asn Tyr Phe Asp Met Gly Ala Asp Leu Gln Trp 975 980 985 caa cct agc cgc agg acc ctg gac gcg gca ggg tgt gag ctt cct tac 12008 Gln Pro Ser Arg Arg Thr Leu Asp Ala Ala Gly Cys Glu Leu Pro Tyr 990 995 1000 1005 gtg gaa gag gtg gat gaa ggc gag gag gag gag ggc gag tac ctg 12053 Val Glu Glu Val Asp Glu Gly Glu Glu Glu Glu Gly Glu Tyr Leu 1010 1015 1020 gaa gac tgatggcgcg acccgtattt ttgctag atg gaa cag cag gca ccg 12104 Glu Asp Met Glu Gln Gln Ala Pro 1025 gac ccc gca atg cgg gcg gcg ctg cag agc cag ccg tcc ggc att 12149 Asp Pro Ala Met Arg Ala Ala Leu Gln Ser Gln Pro Ser Gly Ile 1030 1035 1040 aac tcc tcg gac gat tgg acc cag gcc atg caa cgc atc atg gcg 12194 Asn Ser Ser Asp Asp Trp Thr Gln Ala Met Gln Arg Ile Met Ala 1045 1050 1055 ctg acg acc cgc aac ccc gaa gcc ttt aga cag caa ccc cag gcc 12239 Leu Thr Thr Arg Asn Pro Glu Ala Phe Arg Gln Gln Pro Gln Ala 1060 1065 1070 aac cgc ctt tcg gcc atc ctg gag gcc gta gtt cct tcc cgc tcc 12284 Asn Arg Leu Ser Ala Ile Leu Glu Ala Val Val Pro Ser Arg Ser 1075 1080 1085 aac ccc acc cac gag aag gtc ctg gcc atc gtg aac gcg ctg gtg 12329 Asn Pro Thr His Glu Lys Val Leu Ala Ile Val Asn Ala Leu Val 1090 1095 1100 gag aac aag gcc atc cgt ccc gat gag gcc ggg ctg gta tac aat 12374 Glu Asn Lys Ala Ile Arg Pro Asp Glu Ala Gly Leu Val Tyr Asn 1105 1110 1115 gcc cta ttg gag cgc gtg gcc cgc tac aac agc agc aac gtg cag 12419 Ala Leu Leu Glu Arg Val Ala Arg Tyr Asn Ser Ser Asn Val Gln 1120 1125 1130 acc aac ctg gac cgg atg gtg acc gat gtg cgc gag gcc gtg tct 12464 Thr Asn Leu Asp Arg Met Val Thr Asp Val Arg Glu Ala Val Ser 1135 1140 1145 cag cgc gag cgg ttc cag cgc gat gcc aac ttg ggg tcg ctg gtg 12509 Gln Arg Glu Arg Phe Gln Arg Asp Ala Asn Leu Gly Ser Leu Val 1150 1155 1160 gcg ctg aac gcc ttt ctc agc acc cag cct gcc aac gtg ccc cgc 12554 Ala Leu Asn Ala Phe Leu Ser Thr Gln Pro Ala Asn Val Pro Arg 1165 1170 1175 ggc cag caa gac tat aca aac ttt cta agt gca ctg aga ctc atg 12599 Gly Gln Gln Asp Tyr Thr Asn Phe Leu Ser Ala Leu Arg Leu Met 1180 1185 1190 gta acc gaa gtc cct cag agc gag gtg tac cag tcc gga cca gac 12644 Val Thr Glu Val Pro Gln Ser Glu Val Tyr Gln Ser Gly Pro Asp 1195 1200 1205 tac ttc ttc cag acc agc aga cag ggc ttg cag aca gta aac ctg 12689 Tyr Phe Phe Gln Thr Ser Arg Gln Gly Leu Gln Thr Val Asn Leu 1210 1215 1220 agc cag gct ttc aag aac cta aag ggg ctg tgg gga gtg cat gcc 12734 Ser Gln Ala Phe Lys Asn Leu Lys Gly Leu Trp Gly Val His Ala 1225 1230 1235 cca gta gga gat cgc gcg acc gtg tct agc ttg ctg acc ccc aac 12779 Pro Val Gly Asp Arg Ala Thr Val Ser Ser Leu Leu Thr Pro Asn 1240 1245 1250 tcc cgc cta ctg ctg ctg ctg gtt gcc ccc ttc act gat agc ggt 12824 Ser Arg Leu Leu Leu Leu Leu Val Ala Pro Phe Thr Asp Ser Gly 1255 1260 1265 agc atc gac cgc aac tcc tac ttg ggc tac ctg ctg aac ttg tat 12869 Ser Ile Asp Arg Asn Ser Tyr Leu Gly Tyr Leu Leu Asn Leu Tyr 1270 1275 1280 cgc gag gcc ata gga cag agc cag gtg gac gag cag acc tac caa 12914 Arg Glu Ala Ile Gly Gln Ser Gln Val Asp Glu Gln Thr Tyr Gln 1285 1290 1295 gaa atc acc caa gtg agc cgc gcc ctt ggt cag gaa gat acg ggc 12959 Glu Ile Thr Gln Val Ser Arg Ala Leu Gly Gln Glu Asp Thr Gly 1300 1305 1310 agt ttg gaa gcc acc ttg aac ttc ttg ctg acc aac cgg tcg cag 13004 Ser Leu Glu Ala Thr Leu Asn Phe Leu Leu Thr Asn Arg Ser Gln 1315 1320 1325 aag atc cct cct cag tat gcg ctt acc gcg gag gag gag cgg atc 13049 Lys Ile Pro Pro Gln Tyr Ala Leu Thr Ala Glu Glu Glu Arg Ile 1330 1335 1340 ctg aga tat gtc cag cag agc gtg gga ctg ttc ctg atg caa gag 13094 Leu Arg Tyr Val Gln Gln Ser Val Gly Leu Phe Leu Met Gln Glu 1345 1350 1355 ggg gcg acc cct agt gcc gcg ctg gac atg aca gcc cga aac atg 13139 Gly Ala Thr Pro Ser Ala Ala Leu Asp Met Thr Ala Arg Asn Met 1360 1365 1370 gag ccc agc atg tat gcc agt aac cgg cct ttc atc aac aaa ctg 13184 Glu Pro Ser Met Tyr Ala Ser Asn Arg Pro Phe Ile Asn Lys Leu 1375 1380 1385 ctg gat tac ctg cac agg gcg gcc gcc atg aac tct gat tat ttc 13229 Leu Asp Tyr Leu His Arg Ala Ala Ala Met Asn Ser Asp Tyr Phe 1390 1395 1400 acc aat gct atc ctc aac ccc cac tgg ctg ccc ccg cct gga ttt 13274 Thr Asn Ala Ile Leu Asn Pro His Trp Leu Pro Pro Pro Gly Phe 1405 1410 1415 tac acg ggc gag tac gac atg ccc gac ccc aat gac ggg ttt ctg 13319 Tyr Thr Gly Glu Tyr Asp Met Pro Asp Pro Asn Asp Gly Phe Leu 1420 1425 1430 tgg gac gat gtg gac agc agc ata ttc tcc ccg cct cct ggt tat 13364 Trp Asp Asp Val Asp Ser Ser Ile Phe Ser Pro Pro Pro Gly Tyr 1435 1440 1445 aac act tgg aag aag gaa ggg ggc gat aga aga cac tct tcc gtg 13409 Asn Thr Trp Lys Lys Glu Gly Gly Asp Arg Arg His Ser Ser Val 1450 1455 1460 tcg ctg tcc ggg tcg agg ggt gct gcc gct gcg gtg ccc gag gct 13454 Ser Leu Ser Gly Ser Arg Gly Ala Ala Ala Ala Val Pro Glu Ala 1465 1470 1475 gca agt cct ttc cct agc ctg ccc ttt tct ctg aac agc gtg cgc 13499 Ala Ser Pro Phe Pro Ser Leu Pro Phe Ser Leu Asn Ser Val Arg 1480 1485 1490 agc agt gaa ctg ggg aga ata acc cgc ccg cgc ttg atg ggc gag 13544 Ser Ser Glu Leu Gly Arg Ile Thr Arg Pro Arg Leu Met Gly Glu 1495 1500 1505 gat gag tac ttg aac gac tcc ttg ctt aga ccc gag agg gaa aag 13589 Asp Glu Tyr Leu Asn Asp Ser Leu Leu Arg Pro Glu Arg Glu Lys 1510 1515 1520 aac ttc ccc aac aat ggt ata gag agc ctg gtg gac aag atg agt 13634 Asn Phe Pro Asn Asn Gly Ile Glu Ser Leu Val Asp Lys Met Ser 1525 1530 1535 aga tgg aag act tat gca cag gat cac aaa gat gag cct agg atc 13679 Arg Trp Lys Thr Tyr Ala Gln Asp His Lys Asp Glu Pro Arg Ile 1540 1545 1550 ttg ggg gct gca agc ggg act acc cgt aga cgc cag cgc cat gac 13724 Leu Gly Ala Ala Ser Gly Thr Thr Arg Arg Arg Gln Arg His Asp 1555 1560 1565 aga cag agg ggt ctt gtg tgg gac gat gag gac tcg gcc gat gac 13769 Arg Gln Arg Gly Leu Val Trp Asp Asp Glu Asp Ser Ala Asp Asp 1570 1575 1580 agc agc gtg ttg gac ttg ggt ggg aga gga ggg ggc aac cca ttc 13814 Ser Ser Val Leu Asp Leu Gly Gly Arg Gly Gly Gly Asn Pro Phe 1585 1590 1595 gct cat ctg cgc ccg cac ttt ggg cgc atg ttg taaaagtgaa 13857 Ala His Leu Arg Pro His Phe Gly Arg Met Leu 1600 1605 agtaaaataa aaaaggcaac tcaccaaggc catggcgacg agcgtgcgtt cgttcttttc 13917 tgttatctgt gtctagt atg atg agg cga gcc gtg cta ggc gga gcg gtg 13967 Met Met Arg Arg Ala Val Leu Gly Gly Ala Val 1610 1615 1620 gtg tat ccg gag ggt cct cct cct tcg tac gag agc gtg atg cag 14012 Val Tyr Pro Glu Gly Pro Pro Pro Ser Tyr Glu Ser Val Met Gln 1625 1630 1635 cag cag gcg gcg gcg gtg atg cag ccc tcg ctg gag gct ccc ttt 14057 Gln Gln Ala Ala Ala Val Met Gln Pro Ser Leu Glu Ala Pro Phe 1640 1645 1650 gta ccc ccg cgg tac ctg gcg cct aca gag ggg aga aac agc att 14102 Val Pro Pro Arg Tyr Leu Ala Pro Thr Glu Gly Arg Asn Ser Ile 1655 1660 1665 cgt tac tcg gag ctg gca ccc cag tac gat acc acc agg ttg tat 14147 Arg Tyr Ser Glu Leu Ala Pro Gln Tyr Asp Thr Thr Arg Leu Tyr 1670 1675 1680 ctg gtg gac aac aag tcg gcg gac atc gcc tca ttg aac tat cag 14192 Leu Val Asp Asn Lys Ser Ala Asp Ile Ala Ser Leu Asn Tyr Gln 1685 1690 1695 aac gac cac agc aac ttc ctg acc acg gtg gtg cag aac aat gac 14237 Asn Asp His Ser Asn Phe Leu Thr Thr Val Val Gln Asn Asn Asp 1700 1705 1710 ttt acc ccc acg gag gcc agc acc cag acc atc aac ttt gac gag 14282 Phe Thr Pro Thr Glu Ala Ser Thr Gln Thr Ile Asn Phe Asp Glu 1715 1720 1725 cgg tcg cgg tgg ggc ggt cag ctg aag acc atc atg cac acc aac 14327 Arg Ser Arg Trp Gly Gly Gln Leu Lys Thr Ile Met His Thr Asn 1730 1735 1740 atg ccc aac gtg aac gag tac atg ttc agc aac aag ttc aag gcg 14372 Met Pro Asn Val Asn Glu Tyr Met Phe Ser Asn Lys Phe Lys Ala 1745 1750 1755 cgg gtg atg gtg tca cgc aag aaa cct gaa ggc tat aca ggg gat 14417 Arg Val Met Val Ser Arg Lys Lys Pro Glu Gly Tyr Thr Gly Asp 1760 1765 1770 aaa aat gat aca agt cag gat att ctg gag tat gag tgg ttt gag 14462 Lys Asn Asp Thr Ser Gln Asp Ile Leu Glu Tyr Glu Trp Phe Glu 1775 1780 1785 ttc act tta cca gaa ggc aac ttc tca gcc acc atg acc atc gac 14507 Phe Thr Leu Pro Glu Gly Asn Phe Ser Ala Thr Met Thr Ile Asp 1790 1795 1800 ctg atg aac aat gcc atc att gac aac tac ctg gca gtg ggc aga 14552 Leu Met Asn Asn Ala Ile Ile Asp Asn Tyr Leu Ala Val Gly Arg 1805 1810 1815 cag aat gga gtg ttg gaa agc gac atc ggt gtc aag ttt gat acc 14597 Gln Asn Gly Val Leu Glu Ser Asp Ile Gly Val Lys Phe Asp Thr 1820 1825 1830 agg aac ttc agg ctg ggc tgg gac ccc ata act aaa ctt gtt atg 14642 Arg Asn Phe Arg Leu Gly Trp Asp Pro Ile Thr Lys Leu Val Met 1835 1840 1845 cca gga gtc tac act tat gaa gcc ttc cat cct gat att gtg cta 14687 Pro Gly Val Tyr Thr Tyr Glu Ala Phe His Pro Asp Ile Val Leu 1850 1855 1860 cta cct ggc tgt ggg gtg gac ttt act gag agc cgc ctt agc aac 14732 Leu Pro Gly Cys Gly Val Asp Phe Thr Glu Ser Arg Leu Ser Asn 1865 1870 1875 ttg ctt ggt att agg aag aga cac cca ttc cag gaa ggt ttt aaa 14777 Leu Leu Gly Ile Arg Lys Arg His Pro Phe Gln Glu Gly Phe Lys 1880 1885 1890 att atg tat gag gat ctt gag ggg ggt aat atc ccc gcc ctt ttg 14822 Ile Met Tyr Glu Asp Leu Glu Gly Gly Asn Ile Pro Ala Leu Leu 1895 1900 1905 gat gta gat gcc tat gaa aaa agc aaa aag gaa aac aca gac acc 14867 Asp Val Asp Ala Tyr Glu Lys Ser Lys Lys Glu Asn Thr Asp Thr 1910 1915 1920 acc acc act acc act gtt act act act gaa gta gca act gtt gca 14912 Thr Thr Thr Thr Thr Val Thr Thr Thr Glu Val Ala Thr Val Ala 1925 1930 1935 aga cac gtt gct gaa gta act act gaa gca gca acg gtt gtt gca 14957 Arg His Val Ala Glu Val Thr Thr Glu Ala Ala Thr Val Val Ala 1940 1945 1950 gtg gat cct att gtt gaa gag aac aat aat act gtt aga gga gat 15002 Val Asp Pro Ile Val Glu Glu Asn Asn Asn Thr Val Arg Gly Asp 1955 1960 1965 aat atc cat act gca aat gag atg aaa gca gca gct gat gat aca 15047 Asn Ile His Thr Ala Asn Glu Met Lys Ala Ala Ala Asp Asp Thr 1970 1975 1980 aca gtt gta gtt gtg cct ggc gct gta gtg act gaa acc aaa acc 15092 Thr Val Val Val Val Pro Gly Ala Val Val Thr Glu Thr Lys Thr 1985 1990 1995 aag aca ctc acc att caa cct cta gaa aag gat acc aag gag cgc 15137 Lys Thr Leu Thr Ile Gln Pro Leu Glu Lys Asp Thr Lys Glu Arg 2000 2005 2010 agt tac aat gtc atc tct ggc acc aat gat act gcc tat cgt agt 15182 Ser Tyr Asn Val Ile Ser Gly Thr Asn Asp Thr Ala Tyr Arg Ser 2015 2020 2025 tgg tac cta gca tac aac tat ggc gac cct gaa aaa gga gtc cgc 15227 Trp Tyr Leu Ala Tyr Asn Tyr Gly Asp Pro Glu Lys Gly Val Arg 2030 2035 2040 tcc tgg acg ctg ctc acc act tca gat gtc acc tgc gga gcg gag 15272 Ser Trp Thr Leu Leu Thr Thr Ser Asp Val Thr Cys Gly Ala Glu 2045 2050 2055 caa gta tat tgg tcg ctc cct gac atg atg cag gac ccc gtc acc 15317 Gln Val Tyr Trp Ser Leu Pro Asp Met Met Gln Asp Pro Val Thr 2060 2065 2070 ttc cga tcc acg aga caa gtc agc aac tac ccc gtg gtg ggt gca 15362 Phe Arg Ser Thr Arg Gln Val Ser Asn Tyr Pro Val Val Gly Ala 2075 2080 2085 gag ctc atg ccc gtc ttc tca aag agt ttc tac aac gag caa gcc 15407 Glu Leu Met Pro Val Phe Ser Lys Ser Phe Tyr Asn Glu Gln Ala 2090 2095 2100 gtg tac tcc cag cag ctc cgc cag acc acc tcg ctt acg cac atc 15452 Val Tyr Ser Gln Gln Leu Arg Gln Thr Thr Ser Leu Thr His Ile 2105 2110 2115 ttc gat cgc ttc cct gag aat cag atc ctc atc cgc ccg ccg gcg 15497 Phe Asp Arg Phe Pro Glu Asn Gln Ile Leu Ile Arg Pro Pro Ala 2120 2125 2130 ccc acc att acc acc gtt agt gaa aac gtt cct gct ctc aca gat 15542 Pro Thr Ile Thr Thr Val Ser Glu Asn Val Pro Ala Leu Thr Asp 2135 2140 2145 cac ggg acc ctg ccg ttg cgc agc agt atc cgg gga gtc cag cgc 15587 His Gly Thr Leu Pro Leu Arg Ser Ser Ile Arg Gly Val Gln Arg 2150 2155 2160 gtg acc gtt act gac gcc aga cgc cgc acc tgc ccc tac gtc tac 15632 Val Thr Val Thr Asp Ala Arg Arg Arg Thr Cys Pro Tyr Val Tyr 2165 2170 2175 aag gcc ctg ggc ata gtc gcg ccg cgc gtc ctt tca agc cgc act 15677 Lys Ala Leu Gly Ile Val Ala Pro Arg Val Leu Ser Ser Arg Thr 2180 2185 2190 ttc taaaaaaaaa a atg tcc att ctc atc tca ccc agt aat aac acc 15724 Phe Met Ser Ile Leu Ile Ser Pro Ser Asn Asn Thr 2195 2200 ggt tgg ggg ctg cgc aca ccc acc agg atg tac gga ggc gct cgc 15769 Gly Trp Gly Leu Arg Thr Pro Thr Arg Met Tyr Gly Gly Ala Arg 2205 2210 2215 aaa cgg tct acc cag cac cct gtg cgt gtg cgc ggg cat ttc cgc 15814 Lys Arg Ser Thr Gln His Pro Val Arg Val Arg Gly His Phe Arg 2220 2225 2230 gct ccc tgg ggc gcc ctc aag ggc cgt act cgc act cgg acc acc 15859 Ala Pro Trp Gly Ala Leu Lys Gly Arg Thr Arg Thr Arg Thr Thr 2235 2240 2245 gtc gat gat gtg atc gac cag gtg gtt gca gat gct cgt aat tat 15904 Val Asp Asp Val Ile Asp Gln Val Val Ala Asp Ala Arg Asn Tyr 2250 2255 2260 act cct gct gca cct gca tct act gtg gat gca gtt att gac agc 15949 Thr Pro Ala Ala Pro Ala Ser Thr Val Asp Ala Val Ile Asp Ser 2265 2270 2275 gtg gtg gct gac gct cgc gag tat gct cgc cgg aag agc agg cga 15994 Val Val Ala Asp Ala Arg Glu Tyr Ala Arg Arg Lys Ser Arg Arg 2280 2285 2290 aga cgc atc gcc agg cgc cac cgg gct acc ccc gct atg cga gct 16039 Arg Arg Ile Ala Arg Arg His Arg Ala Thr Pro Ala Met Arg Ala 2295 2300 2305 gca aga gct ctg ctg cgg aga gcc aaa cgc gtg ggg cga aga gcc 16084 Ala Arg Ala Leu Leu Arg Arg Ala Lys Arg Val Gly Arg Arg Ala 2310 2315 2320 atg ctt aga gcg gcc agg cgc gcg gct tca ggg gcc agc gca ggc 16129 Met Leu Arg Ala Ala Arg Arg Ala Ala Ser Gly Ala Ser Ala Gly 2325 2330 2335 aga tcc cgc agg cgc gcg gcc acg gcg gca gca gcg gcc att gcc 16174 Arg Ser Arg Arg Arg Ala Ala Thr Ala Ala Ala Ala Ala Ile Ala 2340 2345 2350 aac atg gcc caa ccg cga aga ggc aat gtg tac tgg gtg cgc gat 16219 Asn Met Ala Gln Pro Arg Arg Gly Asn Val Tyr Trp Val Arg Asp 2355 2360 2365 gcc act acc ggc cag cgc gtg ccc gtg cgc acc cgt ccc cct cgc 16264 Ala Thr Thr Gly Gln Arg Val Pro Val Arg Thr Arg Pro Pro Arg 2370 2375 2380 act tagaagatac tgagcagtct ccgatgttgt gtcccagcgg cgagg atg tcc 16318 Thr Met Ser 2385 aag cgc aaa tac aag gaa gag atg ctc cag gtc atc gcg cct gaa 16363 Lys Arg Lys Tyr Lys Glu Glu Met Leu Gln Val Ile Ala Pro Glu 2390 2395 2400 atc tac ggt cca ccg gtg aag gat gaa aaa aag ccc cgc aaa atc 16408 Ile Tyr Gly Pro Pro Val Lys Asp Glu Lys Lys Pro Arg Lys Ile 2405 2410 2415 aag cgg gtc aaa aag gac aaa aag gaa gaa gat ggc gat gat ggg 16453 Lys Arg Val Lys Lys Asp Lys Lys Glu Glu Asp Gly Asp Asp Gly 2420 2425 2430 ctg gta gag ttt gtg cgc gag ttc gct cca agg cgg cgc gta cag 16498 Leu Val Glu Phe Val Arg Glu Phe Ala Pro Arg Arg Arg Val Gln 2435 2440 2445 tgg cgc ggg cgc aaa gtg cgg ccg gtg ctg aga ccc gga acc acg 16543 Trp Arg Gly Arg Lys Val Arg Pro Val Leu Arg Pro Gly Thr Thr 2450 2455 2460 gtg gtc ttc acg ccc ggc gag cgc tcc agc act act ttt aaa cgc 16588 Val Val Phe Thr Pro Gly Glu Arg Ser Ser Thr Thr Phe Lys Arg 2465 2470 2475 tcc tat gat gag gtg tac ggg gat gat gat att ctg gag cag gcg 16633 Ser Tyr Asp Glu Val Tyr Gly Asp Asp Asp Ile Leu Glu Gln Ala 2480 2485 2490 gcc gac cgc ctg ggc gag ttt gct tat ggc aaa cgc agc cgc tcc 16678 Ala Asp Arg Leu Gly Glu Phe Ala Tyr Gly Lys Arg Ser Arg Ser 2495 2500 2505 agt ccc aag gat gag gcg gtg tcc ata ccc ttg gat cat gga aat 16723 Ser Pro Lys Asp Glu Ala Val Ser Ile Pro Leu Asp His Gly Asn 2510 2515 2520 ccc acc cca agt cta aaa cca gtc acc ctg cag caa gtg cta ccc 16768 Pro Thr Pro Ser Leu Lys Pro Val Thr Leu Gln Gln Val Leu Pro 2525 2530 2535 gtg cct gca cgg aga ggt gtc aag cga gaa ggc gag gat ctg tat 16813 Val Pro Ala Arg Arg Gly Val Lys Arg Glu Gly Glu Asp Leu Tyr 2540 2545 2550 ccc acc atg caa ctg atg gtg ccc aag cgc cag aag ctg gag gac 16858 Pro Thr Met Gln Leu Met Val Pro Lys Arg Gln Lys Leu Glu Asp 2555 2560 2565 gtg ctg gag aaa atg aaa gtg gat ccc gat atc cag cct gaa gtt 16903 Val Leu Glu Lys Met Lys Val Asp Pro Asp Ile Gln Pro Glu Val 2570 2575 2580 aaa gtc aga ccc atc aag cag gtg gcg ccc ggt ctg gga gtg caa 16948 Lys Val Arg Pro Ile Lys Gln Val Ala Pro Gly Leu Gly Val Gln 2585 2590 2595 acc gtg gac atc aag att ccc acc gag tcc atg gaa gtc cag act 16993 Thr Val Asp Ile Lys Ile Pro Thr Glu Ser Met Glu Val Gln Thr 2600 2605 2610 gaa cct gca aag ccc gca gcc acc tct att gag gtg cag acg gat 17038 Glu Pro Ala Lys Pro Ala Ala Thr Ser Ile Glu Val Gln Thr Asp 2615 2620 2625 cct tgg ata ccc gcg ccc gtt gca acc acc gcc agt acc gcc cga 17083 Pro Trp Ile Pro Ala Pro Val Ala Thr Thr Ala Ser Thr Ala Arg 2630 2635 2640 aga ccc cgg cga aag tat ggt cct gcg agt ctg ctg atg ccc aac 17128 Arg Pro Arg Arg Lys Tyr Gly Pro Ala Ser Leu Leu Met Pro Asn 2645 2650 2655 tat gct ctg cac cca tcc att att cca act ccg ggt tac cga ggc 17173 Tyr Ala Leu His Pro Ser Ile Ile Pro Thr Pro Gly Tyr Arg Gly 2660 2665 2670 act cgc tac tac cgc agc cgg agc acc act tcc cgc cgt cgc aaa 17218 Thr Arg Tyr Tyr Arg Ser Arg Ser Thr Thr Ser Arg Arg Arg Lys 2675 2680 2685 aca cct gca agc cgc agt cgc cgt cgc cgc cgc cgc acc gcc agc 17263 Thr Pro Ala Ser Arg Ser Arg Arg Arg Arg Arg Arg Thr Ala Ser 2690 2695 2700 aaa ctg act ccc gcc gct ttg gtg cgg agg gtg tat cgc gat ggc 17308 Lys Leu Thr Pro Ala Ala Leu Val Arg Arg Val Tyr Arg Asp Gly 2705 2710 2715 cgc gca gag ccc ctg atg ctg ccg cgc gca cgc tac cat cca agc 17353 Arg Ala Glu Pro Leu Met Leu Pro Arg Ala Arg Tyr His Pro Ser 2720 2725 2730 atc acc act taatgactgt tgccgctgcc tccttgcaga t atg gcc ctc act 17405 Ile Thr Thr Met Ala Leu Thr 2735 tgc cgc ctt cgc gtc ccc att act ggc tac cga gga aga aac tcg 17450 Cys Arg Leu Arg Val Pro Ile Thr Gly Tyr Arg Gly Arg Asn Ser 2740 2745 2750 cgc cgt aga agg atg ttg ggt agc ggg atg cgt cgc cac agg cgg 17495 Arg Arg Arg Arg Met Leu Gly Ser Gly Met Arg Arg His Arg Arg 2755 2760 2765 cgg cgc gct atc agc aag agg ctg ggg ggt ggc ttt ctg acc gct 17540 Arg Arg Ala Ile Ser Lys Arg Leu Gly Gly Gly Phe Leu Thr Ala 2770 2775 2780 ttg att ccc att atc gcc gcg gcg atc ggg gcg gta cca ggc ata 17585 Leu Ile Pro Ile Ile Ala Ala Ala Ile Gly Ala Val Pro Gly Ile 2785 2790 2795 gct tcc gtg gcg gtt cag gcc tcg cag cgc cac tgacattgga 17628 Ala Ser Val Ala Val Gln Ala Ser Gln Arg His 2800 2805 aaaacactta taaataaaat agaatggact ctgacgctcc tggtcctgtg actatgtttt 17688 tgtagag atg gaa gac atc aat ttt tca tcc ctg gct ccg cga cac 17734 Met Glu Asp Ile Asn Phe Ser Ser Leu Ala Pro Arg His 2810 2815 2820 ggc acg agg ccg tac atg ggc acc tgg agc gac atc ggc acc agc 17779 Gly Thr Arg Pro Tyr Met Gly Thr Trp Ser Asp Ile Gly Thr Ser 2825 2830 2835 caa ctg aac ggg ggc gcc ttc aat tgg agc agt atc tgg agc ggg 17824 Gln Leu Asn Gly Gly Ala Phe Asn Trp Ser Ser Ile Trp Ser Gly 2840 2845 2850 ctt aaa aat ttt ggc tct gcc ata aaa acc tat ggg aac aaa gct 17869 Leu Lys Asn Phe Gly Ser Ala Ile Lys Thr Tyr Gly Asn Lys Ala 2855 2860 2865 tgg aac agc agc aca ggg cag gcg ctg agg aat aag ctt aaa gag 17914 Trp Asn Ser Ser Thr Gly Gln Ala Leu Arg Asn Lys Leu Lys Glu 2870 2875 2880 cag aac ttc cag cag aag gtg gtc gat ggg atc gcc tct ggc atc 17959 Gln Asn Phe Gln Gln Lys Val Val Asp Gly Ile Ala Ser Gly Ile 2885 2890 2895 aat ggg gta gtg gat ctg gcc aac cag gcc gtg cag aaa cag ata 18004 Asn Gly Val Val Asp Leu Ala Asn Gln Ala Val Gln Lys Gln Ile 2900 2905 2910 aac agc cgc ctg gac ccg ccg ccc gca gcc cct ggc gaa atg gaa 18049 Asn Ser Arg Leu Asp Pro Pro Pro Ala Ala Pro Gly Glu Met Glu 2915 2920 2925 gtg gag gaa gag ctc cct ccc ctg gaa aag cgg gga gac aag cgc 18094 Val Glu Glu Glu Leu Pro Pro Leu Glu Lys Arg Gly Asp Lys Arg 2930 2935 2940 ccg cgt ccc gat atg gag gag acg ctg gtg acg cgg gga gac gaa 18139 Pro Arg Pro Asp Met Glu Glu Thr Leu Val Thr Arg Gly Asp Glu 2945 2950 2955 ccg cct cca tat gag gag gcg ata aag ctt gga atg ccc act acc 18184 Pro Pro Pro Tyr Glu Glu Ala Ile Lys Leu Gly Met Pro Thr Thr 2960 2965 2970 agg cct ata gct ccc atg gcc acc ggg gta atg aaa cct tct cag 18229 Arg Pro Ile Ala Pro Met Ala Thr Gly Val Met Lys Pro Ser Gln 2975 2980 2985 tcg cat cga ccc gcc acc ctg gac ttg cct cct gcc cct gct gct 18274 Ser His Arg Pro Ala Thr Leu Asp Leu Pro Pro Ala Pro Ala Ala 2990 2995 3000 gca gcg ccc gct cca aag cct gtc gct acc ccg aag cct acc tcc 18319 Ala Ala Pro Ala Pro Lys Pro Val Ala Thr Pro Lys Pro Thr Ser 3005 3010 3015 gta cag ccc gtc gcc gta gcc aga ccg cgt cct ggg ggc act ccg 18364 Val Gln Pro Val Ala Val Ala Arg Pro Arg Pro Gly Gly Thr Pro 3020 3025 3030 cgc ccg aat gca aac tgg cag agt act ctg aac agc atc gtg ggt 18409 Arg Pro Asn Ala Asn Trp Gln Ser Thr Leu Asn Ser Ile Val Gly 3035 3040 3045 ttg ggc gtg cag agt gta aag cgc cgt cgc tgc tat taattaaata 18455 Leu Gly Val Gln Ser Val Lys Arg Arg Arg Cys Tyr 3050 3055 tggagtagcg cttaacttgc ttgtctgtgt gtatgtgtca tcaccacgcc gccgcagcag 18515 cagaggagaa aggaagaggt cgcgcgccga ggctgagttg ctttcaag atg gcc acc 18572 Met Ala Thr 3060 cca tcg atg ctg ccc cag tgg gca tac atg cac atc gcc gga cag 18617 Pro Ser Met Leu Pro Gln Trp Ala Tyr Met His Ile Ala Gly Gln 3065 3070 3075 gat gct tcg gag tac ctg agt ccg ggt ctg gtg cag ttc gcc cgt 18662 Asp Ala Ser Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala Arg 3080 3085 3090 gcc aca gat acc tac ttc aat ctg ggg aac aag ttt agg aac ccc 18707 Ala Thr Asp Thr Tyr Phe Asn Leu Gly Asn Lys Phe Arg Asn Pro 3095 3100 3105 acc gtg gcc ccc acc cac gat gtg acc acc gac cga agc cag cgg 18752 Thr Val Ala Pro Thr His Asp Val Thr Thr Asp Arg Ser Gln Arg 3110 3115 3120 ctg atg ctg cgc ttt gtg ccc gtt gat cgt gag gac aat act tac 18797 Leu Met Leu Arg Phe Val Pro Val Asp Arg Glu Asp Asn Thr Tyr 3125 3130 3135 tcg tac aaa gtt cgc tac aca ctg gct gtg ggc gac aac aga gtg 18842 Ser Tyr Lys Val Arg Tyr Thr Leu Ala Val Gly Asp Asn Arg Val 3140 3145 3150 cta gat atg gcc agc acc ttc ttt gac atc aga ggg gtg ctt gat 18887 Leu Asp Met Ala Ser Thr Phe Phe Asp Ile Arg Gly Val Leu Asp 3155 3160 3165 aga ggt ccc agc ttc aag ccc tac tct ggc aca gct tac aac tcc 18932 Arg Gly Pro Ser Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser 3170 3175 3180 ctg gct cct aag gga gct ccc aac cct agc cag tgg cta gaa tct 18977 Leu Ala Pro Lys Gly Ala Pro Asn Pro Ser Gln Trp Leu Glu Ser 3185 3190 3195 act aca gct gat gaa act acc act act act aca cat acg ttt ggt 19022 Thr Thr Ala Asp Glu Thr Thr Thr Thr Thr Thr His Thr Phe Gly 3200 3205 3210 atg gct tct atg aag ggg tat gac att acc aaa gac ggt tta caa 19067 Met Ala Ser Met Lys Gly Tyr Asp Ile Thr Lys Asp Gly Leu Gln 3215 3220 3225 att gga aaa gaa gta act gcc act ggc gat gaa aaa cca att tat 19112 Ile Gly Lys Glu Val Thr Ala Thr Gly Asp Glu Lys Pro Ile Tyr 3230 3235 3240 gca gat aaa aaa ttt caa cca gaa ccc caa gta ggg gaa gaa tct 19157 Ala Asp Lys Lys Phe Gln Pro Glu Pro Gln Val Gly Glu Glu Ser 3245 3250 3255 tgg act gac act gat gga aca aat gaa aaa ttt gga ggc aga act 19202 Trp Thr Asp Thr Asp Gly Thr Asn Glu Lys Phe Gly Gly Arg Thr 3260 3265 3270 ctt aaa agt gct acc aat atg aaa cca tgt tat gga tcg ttc gct 19247 Leu Lys Ser Ala Thr Asn Met Lys Pro Cys Tyr Gly Ser Phe Ala 3275 3280 3285 aga ccc aca aac aag gaa gga ggt cag gcc aaa acc aga aaa gtc 19292 Arg Pro Thr Asn Lys Glu Gly Gly Gln Ala Lys Thr Arg Lys Val 3290 3295 3300 cct gcg gct gaa gag ggg gga gct gaa act gaa gag cca gat att 19337 Pro Ala Ala Glu Glu Gly Gly Ala Glu Thr Glu Glu Pro Asp Ile 3305 3310 3315 gat atg gtg ttt tat gat gat aga caa gct gcc gat cct gct ttg 19382 Asp Met Val Phe Tyr Asp Asp Arg Gln Ala Ala Asp Pro Ala Leu 3320 3325 3330 gcg cct gaa gtt gtt ctt tac act gaa aat gta aat ttg gaa act 19427 Ala Pro Glu Val Val Leu Tyr Thr Glu Asn Val Asn Leu Glu Thr 3335 3340 3345 cct gac acc cat att gta tac aag ccg ggt act tca gat gtc agt 19472 Pro Asp Thr His Ile Val Tyr Lys Pro Gly Thr Ser Asp Val Ser 3350 3355 3360 tca cat gaa aat ttg gga cag caa gct atg cct aac aga ccc aat 19517 Ser His Glu Asn Leu Gly Gln Gln Ala Met Pro Asn Arg Pro Asn 3365 3370 3375 tac att gga ttt aga gat aac ttt gtt ggg ctt atg tac tac aat 19562 Tyr Ile Gly Phe Arg Asp Asn Phe Val Gly Leu Met Tyr Tyr Asn 3380 3385 3390 agt act ggc aac atg ggt gtg ttg gca ggg cag gca tca cag cta 19607 Ser Thr Gly Asn Met Gly Val Leu Ala Gly Gln Ala Ser Gln Leu 3395 3400 3405 aat gca gta gtt gac ttg caa gat aga aac act gag cta tcg tac 19652 Asn Ala Val Val Asp Leu Gln Asp Arg Asn Thr Glu Leu Ser Tyr 3410 3415 3420 cag ctt ttg ctt gat tcc ctg ggt gac aga act cga tat ttc agc 19697 Gln Leu Leu Leu Asp Ser Leu Gly Asp Arg Thr Arg Tyr Phe Ser 3425 3430 3435 atg tgg aat cag gct gtg gat agt tat gac ccc gat gtg cgc att 19742 Met Trp Asn Gln Ala Val Asp Ser Tyr Asp Pro Asp Val Arg Ile 3440 3445 3450 att gaa aat cat ggc ata gag gat gaa ttg cca aat tat tgt ttt 19787 Ile Glu Asn His Gly Ile Glu Asp Glu Leu Pro Asn Tyr Cys Phe 3455 3460 3465 cct ttg gat gga att ggg cct ggc aag tcc tat caa gga att aaa 19832 Pro Leu Asp Gly Ile Gly Pro Gly Lys Ser Tyr Gln Gly Ile Lys 3470 3475 3480 gag aaa act ggc gaa gac aaa aaa tgg gaa aaa gac ggt act cag 19877 Glu Lys Thr Gly Glu Asp Lys Lys Trp Glu Lys Asp Gly Thr Gln 3485 3490 3495 gcc aat tcc aat gaa ata gcc ata ggt aat aac ctg gct atg gaa 19922 Ala Asn Ser Asn Glu Ile Ala Ile Gly Asn Asn Leu Ala Met Glu 3500 3505 3510 att aat atc cag gct aac ctc tgg aga agt ttt ctg tac tcc aac 19967 Ile Asn Ile Gln Ala Asn Leu Trp Arg Ser Phe Leu Tyr Ser Asn 3515 3520 3525 gtg gct ctg tac ctt cca gat gct tac aag tac acg cca gcc aac 20012 Val Ala Leu Tyr Leu Pro Asp Ala Tyr Lys Tyr Thr Pro Ala Asn 3530 3535 3540 att act ttg cct gcc aat acc aac acc tat gaa tac atg aac ggg 20057 Ile Thr Leu Pro Ala Asn Thr Asn Thr Tyr Glu Tyr Met Asn Gly 3545 3550 3555 cga gtg gtg gca cca tct ttg gtt gat tca tac atc aac att ggt 20102 Arg Val Val Ala Pro Ser Leu Val Asp Ser Tyr Ile Asn Ile Gly 3560 3565 3570 gcc agg tgg tct ctt gac cca atg gac aat gtg aac ccc ttc aat 20147 Ala Arg Trp Ser Leu Asp Pro Met Asp Asn Val Asn Pro Phe Asn 3575 3580 3585 cac cac cga aac gct ggg ctg cgt tac aga tcc atg ctt ctg ggc 20192 His His Arg Asn Ala Gly Leu Arg Tyr Arg Ser Met Leu Leu Gly 3590 3595 3600 aat ggt cgc tat gtg cct ttc cac atc caa gtg cct caa aaa ttc 20237 Asn Gly Arg Tyr Val Pro Phe His Ile Gln Val Pro Gln Lys Phe 3605 3610 3615 ttt gct atc aaa aac ctg ctt ctc ctc ccc gga tca tac acc tat 20282 Phe Ala Ile Lys Asn Leu Leu Leu Leu Pro Gly Ser Tyr Thr Tyr 3620 3625 3630 gag tgg aac ttc aga aag gat gtg aat atg gtt ctg cag agt tcc 20327 Glu Trp Asn Phe Arg Lys Asp Val Asn Met Val Leu Gln Ser Ser 3635 3640 3645 ctt ggt aat gat ctc aga act gat gga gcc agc atc agt ttt acc 20372 Leu Gly Asn Asp Leu Arg Thr Asp Gly Ala Ser Ile Ser Phe Thr 3650 3655 3660 agc atc aac ctc tac gcc acc ttc ttc ccc atg gct cac aac act 20417 Ser Ile Asn Leu Tyr Ala Thr Phe Phe Pro Met Ala His Asn Thr 3665 3670 3675 gct tcc acc ctt gaa gcc atg ctg cgc aat gac acc aat gac cag 20462 Ala Ser Thr Leu Glu Ala Met Leu Arg Asn Asp Thr Asn Asp Gln 3680 3685 3690 tca ttc aat gac tac ctt tct gca gct aac atg ctc tac cca att 20507 Ser Phe Asn Asp Tyr Leu Ser Ala Ala Asn Met Leu Tyr Pro Ile 3695 3700 3705 cca gca aat gct acc aac atc ccc atc tca att ccc tct cgc aac 20552 Pro Ala Asn Ala Thr Asn Ile Pro Ile Ser Ile Pro Ser Arg Asn 3710 3715 3720 tgg gct gcc ttc agg ggc tgg tca ttc acc aga ctc aaa aca aag 20597 Trp Ala Ala Phe Arg Gly Trp Ser Phe Thr Arg Leu Lys Thr Lys 3725 3730 3735 gag act ccc tct ttg gga tca gga ttc gat ccc tac ttt gtt tac 20642 Glu Thr Pro Ser Leu Gly Ser Gly Phe Asp Pro Tyr Phe Val Tyr 3740 3745 3750 tct ggt tct att ccc tac ctg gat ggc acc ttc tac ctc aac cac 20687 Ser Gly Ser Ile Pro Tyr Leu Asp Gly Thr Phe Tyr Leu Asn His 3755 3760 3765 act ttc aag aag gtg tcc atc atg ttt gac tcc tca gtc agc tgg 20732 Thr Phe Lys Lys Val Ser Ile Met Phe Asp Ser Ser Val Ser Trp 3770 3775 3780 cca ggc aat gac aga ttg cta act cca aat gag ttc gaa atc aag 20777 Pro Gly Asn Asp Arg Leu Leu Thr Pro Asn Glu Phe Glu Ile Lys 3785 3790 3795 cgc act gtg gat gga gaa ggg tac aat gtg gct caa tgc aac atg 20822 Arg Thr Val Asp Gly Glu Gly Tyr Asn Val Ala Gln Cys Asn Met 3800 3805 3810 acc aag gat tgg ttc ctg gtt cag atg ctt gcc aac tat aac att 20867 Thr Lys Asp Trp Phe Leu Val Gln Met Leu Ala Asn Tyr Asn Ile 3815 3820 3825 ggc tac cag ggc ttt tac atc cca gag ggg tac aag gat cgc atg 20912 Gly Tyr Gln Gly Phe Tyr Ile Pro Glu Gly Tyr Lys Asp Arg Met 3830 3835 3840 tat tcc ttc ttc aga aac ttc cag ccc atg agc aga cag gtg gtt 20957 Tyr Ser Phe Phe Arg Asn Phe Gln Pro Met Ser Arg Gln Val Val 3845 3850 3855 gat gaa gtt aat tac aag gag tac caa gcc gtc aca ctt gct tac 21002 Asp Glu Val Asn Tyr Lys Glu Tyr Gln Ala Val Thr Leu Ala Tyr 3860 3865 3870 caa cac aac aac tct ggc ttt gtg ggt tac ctt gcg ccc act atg 21047 Gln His Asn Asn Ser Gly Phe Val Gly Tyr Leu Ala Pro Thr Met 3875 3880 3885 agg cag gga gaa cct tac ccc gct aac tac cca tac ccc cta atc 21092 Arg Gln Gly Glu Pro Tyr Pro Ala Asn Tyr Pro Tyr Pro Leu Ile 3890 3895 3900 gga acc act gct gtc aag agt gtt acc cag aaa aag ttc ctg tgc 21137 Gly Thr Thr Ala Val Lys Ser Val Thr Gln Lys Lys Phe Leu Cys 3905 3910 3915 gac agg acc atg tgg cgc atc ccc ttc tcc agc aac ttc atg tcc 21182 Asp Arg Thr Met Trp Arg Ile Pro Phe Ser Ser Asn Phe Met Ser 3920 3925 3930 atg ggt gcc ctt acc gac ctg gga cag aac atg ctt tat gcc aac 21227 Met Gly Ala Leu Thr Asp Leu Gly Gln Asn Met Leu Tyr Ala Asn 3935 3940 3945 tca gcc cat gcg ctg gac atg act ttt gag gtg gat ccc atg gat 21272 Ser Ala His Ala Leu Asp Met Thr Phe Glu Val Asp Pro Met Asp 3950 3955 3960 gag ccc acc ctg ctt tat gtt ctt ttc gaa gtc ttc gac gtg gtc 21317 Glu Pro Thr Leu Leu Tyr Val Leu Phe Glu Val Phe Asp Val Val 3965 3970 3975 aga gtg cac cag cca cac cgc ggc gtc atc gag gct gtc tac ctg 21362 Arg Val His Gln Pro His Arg Gly Val Ile Glu Ala Val Tyr Leu 3980 3985 3990 cgt acc ccg ttc tca gct ggt aac gcc acc aca taaagaagct 21405 Arg Thr Pro Phe Ser Ala Gly Asn Ala Thr Thr 3995 4000 tcttgcttct tgcaagcagc tgtc atg gcc tgt ggg tcc ggc aac gga tcc 21456 Met Ala Cys Gly Ser Gly Asn Gly Ser 4005 4010 agc gag caa gag ctc agg gcc att gct aga gac ctg ggc tgt gga 21501 Ser Glu Gln Glu Leu Arg Ala Ile Ala Arg Asp Leu Gly Cys Gly 4015 4020 4025 ccc tat ttc ctg gga acc ttt gat aag cgc ttc ccg ggg ttc atg 21546 Pro Tyr Phe Leu Gly Thr Phe Asp Lys Arg Phe Pro Gly Phe Met 4030 4035 4040 gct ccc gac aag ctc gcc tgt gcc att gtt aat acg gcc ggt cgc 21591 Ala Pro Asp Lys Leu Ala Cys Ala Ile Val Asn Thr Ala Gly Arg 4045 4050 4055 gag acg ggg gga gag cac tgg ctg gct ttt ggt tgg aat ccg cgc 21636 Glu Thr Gly Gly Glu His Trp Leu Ala Phe Gly Trp Asn Pro Arg 4060 4065 4070 tcc aac acc tgc tac ctt ttt gat cct ttt ggc ttc tcg gat gag 21681 Ser Asn Thr Cys Tyr Leu Phe Asp Pro Phe Gly Phe Ser Asp Glu 4075 4080 4085 cgc ctc aag caa atc tac cag ttt gag tat gag ggt ctc ctg cgc 21726 Arg Leu Lys Gln Ile Tyr Gln Phe Glu Tyr Glu Gly Leu Leu Arg 4090 4095 4100 cgc agt gcc ctg gct acc aag gat cgc tgt gtc acc ctg gaa aag 21771 Arg Ser Ala Leu Ala Thr Lys Asp Arg Cys Val Thr Leu Glu Lys 4105 4110 4115 tcc acc cag acc gtg cag ggc ccg cgc tcc gca gcc tgt gga ctt 21816 Ser Thr Gln Thr Val Gln Gly Pro Arg Ser Ala Ala Cys Gly Leu 4120 4125 4130 ttt tgc tgc atg ttc ctc cac gct ttt gtg cat tgg ccc gac cgc 21861 Phe Cys Cys Met Phe Leu His Ala Phe Val His Trp Pro Asp Arg 4135 4140 4145 ccc atg gac gga aac ccc acc atg aag ttg ttg act ggg gtg ccc 21906 Pro Met Asp Gly Asn Pro Thr Met Lys Leu Leu Thr Gly Val Pro 4150 4155 4160 aac agc atg ctc caa tca ccc caa gtc cag ccc acc ctg cgc cac 21951 Asn Ser Met Leu Gln Ser Pro Gln Val Gln Pro Thr Leu Arg His 4165 4170 4175 aac cag gag gcg ctc tac cgc ttc ctc aat acc cac tca tct tac 21996 Asn Gln Glu Ala Leu Tyr Arg Phe Leu Asn Thr His Ser Ser Tyr 4180 4185 4190 ttt cgt tct cac cgc gcg cgc atc gaa aag gct acc gcg ttt gac 22041 Phe Arg Ser His Arg Ala Arg Ile Glu Lys Ala Thr Ala Phe Asp 4195 4200 4205 cgt atg gat atg caa taataagtca tgtaaaaacc gtgttcaaat aaacagcact 22096 Arg Met Asp Met Gln 4210 ttatttttta catgcactgt ggctctgggt tgctcattca ttcatcattc actcagaagt 22156 cgaaggggtt ctggcgggaa tcagcgtgac ccgctggcag ggatacgttg cggaactgga 22216 acctgttctg ccacttgaac tcggggatca ccagcttggg aactggaatc tcggggaagg 22276 tgtcttgcca cagctttctg gtcagttgca gagcgccgag caggtcagga gcagagatct 22336 tgaaatcaca gttggggccg gcattctggg tgcggtagtt gcggtacact gggttgcagc 22396 actggaacac catcagggcg gggtgtctca cgctcgccag cacggtcggg tcactgatgg 22456 tagacacatc caagtcttca gcattggcca ttccaaaggg ggtcatctta caggtctgcc 22516 tgcccatcac gggagcgcag ccgggcttgt ggttgcaatc gcagtgaatg gggatcagca 22576 tcatcctggc ctggtcgggg gttatccctg gataaaccgc cttcataaag gcttcgtact 22636 gcttgaaagc ttcctgggcc ttgcttccct cggtgtagaa catcccacag gacttgctgg 22696 aaaactgatt agtagcacag ttggcatcat tcacacagca gcgggcatcg ttgttggcca 22756 gctggaccac attcctgccc cagcggttct gggtgatctt ggctcggtct gggttctcct 22816 tcatcgcgcg ctgcccgttc tcgctcgcca catccatctc gatgatgtga tccttctgga 22876 tcatgatagt gccatgcagg catttcacct tgccttcata atcggtgcag ccatgagccc 22936 acagagcgca cccggtgcac tcccaattgt tgtgggcgat ctcagaataa gaatgcacca 22996 atccctgcag gaatcttccc atcatcgcag tcagggtctt caagctggta aaggtcagcg 23056 ggatgccgcg gtgctcctcg ttcacatact ggtggcagat acgcctgtac tgctcgtgct 23116 gttcgggcat cagcttgaaa gaggttctca ggtcattatc cagcctgtac ctctccatca 23176 gtacggccat tacttccatg cccttctccc aggcagagac caggggcagg ctcatgggat 23236 tcctaacagc aagagcagca gatgcagctc ctttagccag agggtcattc ttgtcaatct 23296 tctcaacact tctcttgcca tccttctcag tgatgcgcac gggtgggtag ctgaaaccca 23356 cgaccaccag ctctgcctgt tctctttctt cttcgctgtc ctggctgatg tcttgcagag 23416 ggacatgttt ggtcttcctg ggcttcttct tgggagggat cgggggaggg ctgttgctcc 23476 gctccggaga cagggaggac cgcgaagttt cgctcaccag taccacctgg ctctcggtag 23536 aagaaccgga ccccacgcgg cggtaggtgt tcctcttcgg gggcagaggt ggaggcgact 23596 gcgatggact gcgatccggc ctgggaggcg gatggctggc agagcctctt ccgcgttcgg 23656 gggtgtgttc ccggtggcgg tcgcttgact gatttcctcc gcggctggcc attgtgttct 23716 cctaggcaga gaaacaacag ac atg gag act cag cca tcg ctg cca aca 23765 Met Glu Thr Gln Pro Ser Leu Pro Thr 4215 4220 ccg ctg caa gcg cca tca cac ctc gcc ccc agc agc gac gag gag 23810 Pro Leu Gln Ala Pro Ser His Leu Ala Pro Ser Ser Asp Glu Glu 4225 4230 4235 gag agc tta acc acc cca cca ccc agt ccc gcc acc acc acc tct 23855 Glu Ser Leu Thr Thr Pro Pro Pro Ser Pro Ala Thr Thr Thr Ser 4240 4245 4250 acc cta gag gat gag gag gag gtc gac gca ccc cag gag atg cag 23900 Thr Leu Glu Asp Glu Glu Glu Val Asp Ala Pro Gln Glu Met Gln 4255 4260 4265 gat atg gag gat gag aaa gcg gaa gag att gag gca gat gtc gag 23945 Asp Met Glu Asp Glu Lys Ala Glu Glu Ile Glu Ala Asp Val Glu 4270 4275 4280 cag gac ccg ggc tat gtg aca ccg gcg gag cac gag gag gag ctg 23990 Gln Asp Pro Gly Tyr Val Thr Pro Ala Glu His Glu Glu Glu Leu 4285 4290 4295 aga cgc ttt cta gac aga gag gat gac aac cgc cca gag cag aaa 24035 Arg Arg Phe Leu Asp Arg Glu Asp Asp Asn Arg Pro Glu Gln Lys 4300 4305 4310 gca gat ggc gat cac cag gag gct ggg ctc ggg gat cat gtc gcc 24080 Ala Asp Gly Asp His Gln Glu Ala Gly Leu Gly Asp His Val Ala 4315 4320 4325 gaa tac ctc acc ggg ctt ggc ggg gag gac gtg ctc ctc aaa cat 24125 Glu Tyr Leu Thr Gly Leu Gly Gly Glu Asp Val Leu Leu Lys His 4330 4335 4340 cta gca agg cag tcg atc ata gtt aaa gac gca ctg ctc gac cgc 24170 Leu Ala Arg Gln Ser Ile Ile Val Lys Asp Ala Leu Leu Asp Arg 4345 4350 4355 acc gaa gtg ccc atc agt gtg gaa gag ctc agc cgc gcc tac gag 24215 Thr Glu Val Pro Ile Ser Val Glu Glu Leu Ser Arg Ala Tyr Glu 4360 4365 4370 ctc aac ctg ttc tcg cct cgg ctg ccc ccc aaa cgt cag cca aac 24260 Leu Asn Leu Phe Ser Pro Arg Leu Pro Pro Lys Arg Gln Pro Asn 4375 4380 4385 ggc acc tgt gag ccc aac cct cgc ctc aac ttc tat ccg gcc ttt 24305 Gly Thr Cys Glu Pro Asn Pro Arg Leu Asn Phe Tyr Pro Ala Phe 4390 4395 4400 gct gtc cca gaa gtg ctt gct acc tac cac atc ttt ttc aag aac 24350 Ala Val Pro Glu Val Leu Ala Thr Tyr His Ile Phe Phe Lys Asn 4405 4410 4415 caa aag att cca gtc tcc tgc cgc gcc aac cgc acc cgc gcc gat 24395 Gln Lys Ile Pro Val Ser Cys Arg Ala Asn Arg Thr Arg Ala Asp 4420 4425 4430 gcc ctg ctc aac ttg ggt ccg gga gct cgc tta cct gat ata gct 24440 Ala Leu Leu Asn Leu Gly Pro Gly Ala Arg Leu Pro Asp Ile Ala 4435 4440 4445 tcc ttg gaa gag gtt cca aag atc ttc gag ggt ctg ggc agt gat 24485 Ser Leu Glu Glu Val Pro Lys Ile Phe Glu Gly Leu Gly Ser Asp 4450 4455 4460 gag act cgg gcc gca aat gct ctg caa cag gga gag aat ggc atg 24530 Glu Thr Arg Ala Ala Asn Ala Leu Gln Gln Gly Glu Asn Gly Met 4465 4470 4475 gat gaa cat cac agc gct ctg gtg gag ttg gag gga gac aat gcc 24575 Asp Glu His His Ser Ala Leu Val Glu Leu Glu Gly Asp Asn Ala 4480 4485 4490 cgg ctt gca gtg ctc aag cgc agt atc gag gtc acc cat ttt gcc 24620 Arg Leu Ala Val Leu Lys Arg Ser Ile Glu Val Thr His Phe Ala 4495 4500 4505 tac ccc gct gtc aac ctg ccc ccc aaa gtc atg agc gct gtc atg 24665 Tyr Pro Ala Val Asn Leu Pro Pro Lys Val Met Ser Ala Val Met 4510 4515 4520 gat cag ctg ctc atc aag cgc gca agc ccc ctt ccc gaa gac cag 24710 Asp Gln Leu Leu Ile Lys Arg Ala Ser Pro Leu Pro Glu Asp Gln 4525 4530 4535 aac atg cag gat cca gac gcc tcg gac gag ggc aag ccg gtg gtt 24755 Asn Met Gln Asp Pro Asp Ala Ser Asp Glu Gly Lys Pro Val Val 4540 4545 4550 agt gac gag cag ctg tct cgc tgg ctg ggc acc aac tcc ccg cga 24800 Ser Asp Glu Gln Leu Ser Arg Trp Leu Gly Thr Asn Ser Pro Arg 4555 4560 4565 gac ttg gaa gag agg cgc aag ctt atg atg gct gta gtg cta gtc 24845 Asp Leu Glu Glu Arg Arg Lys Leu Met Met Ala Val Val Leu Val 4570 4575 4580 act gtg gag ctg gag tgt ctc cgc cgc ttt ttc acc gac cct gag 24890 Thr Val Glu Leu Glu Cys Leu Arg Arg Phe Phe Thr Asp Pro Glu 4585 4590 4595 acc ctg cgc aag ctc gag gag aac ctg cac tat act ttc aga cat 24935 Thr Leu Arg Lys Leu Glu Glu Asn Leu His Tyr Thr Phe Arg His 4600 4605 4610 ggt ttc gtg cgc cag gca tgc aag atc tcc aac gtg gag ctc acc 24980 Gly Phe Val Arg Gln Ala Cys Lys Ile Ser Asn Val Glu Leu Thr 4615 4620 4625 aac ctg gtc tcc tac atg ggc att ttg cat gag aac cgc ctg ggg 25025 Asn Leu Val Ser Tyr Met Gly Ile Leu His Glu Asn Arg Leu Gly 4630 4635 4640 cag agc gta ctg cat acc acc ctg aaa ggg gag gcc cgc cgc gac 25070 Gln Ser Val Leu His Thr Thr Leu Lys Gly Glu Ala Arg Arg Asp 4645 4650 4655 tac atc cgc gac tgt gtc tac ctc tac ctc tgc cat acc tgg cag 25115 Tyr Ile Arg Asp Cys Val Tyr Leu Tyr Leu Cys His Thr Trp Gln 4660 4665 4670 act ggc atg ggt gta tgg cag cag tgt ttg gaa gag cag aac ctg 25160 Thr Gly Met Gly Val Trp Gln Gln Cys Leu Glu Glu Gln Asn Leu 4675 4680 4685 aaa gag ctg gac aag ctc ttg cag aga tcc ctc aaa gcc ctg tgg 25205 Lys Glu Leu Asp Lys Leu Leu Gln Arg Ser Leu Lys Ala Leu Trp 4690 4695 4700 aca ggt ttt gac gag cgc acc gtc gcc tca gac ctg gca gac atc 25250 Thr Gly Phe Asp Glu Arg Thr Val Ala Ser Asp Leu Ala Asp Ile 4705 4710 4715 atc ttc ccc gag cgt ctc agg gtt act ctg cgc aac ggc ctg cct 25295 Ile Phe Pro Glu Arg Leu Arg Val Thr Leu Arg Asn Gly Leu Pro 4720 4725 4730 gac ttc atg agc cag agc atg ctt aac aac ttt cgc tct ttc atc 25340 Asp Phe Met Ser Gln Ser Met Leu Asn Asn Phe Arg Ser Phe Ile 4735 4740 4745 ctg gaa cgc tcc ggt atc ctg ccc gcc acc tgc tgc gcg ctg ccc 25385 Leu Glu Arg Ser Gly Ile Leu Pro Ala Thr Cys Cys Ala Leu Pro 4750 4755 4760 tcc gac ttt gtg cct ctc acc tac cgc gag tgc ccc ccg ccg cta 25430 Ser Asp Phe Val Pro Leu Thr Tyr Arg Glu Cys Pro Pro Pro Leu 4765 4770 4775 tgg agc cac tgc tac ctg ttc cgc ctg gcc aac tac ctc tcc tac 25475 Trp Ser His Cys Tyr Leu Phe Arg Leu Ala Asn Tyr Leu Ser Tyr 4780 4785 4790 cac tcg gat gtg atc gag gat gtg agc gga gac ggc ctg ctg gag 25520 His Ser Asp Val Ile Glu Asp Val Ser Gly Asp Gly Leu Leu Glu 4795 4800 4805 tgc cac tgc cgc tgc aat ctc tgc aca ccc cac cgt tcc ctc gcc 25565 Cys His Cys Arg Cys Asn Leu Cys Thr Pro His Arg Ser Leu Ala 4810 4815 4820 tgc aac ccc cag ttg ctg agc gag acc cag atc atc ggc acc ttc 25610 Cys Asn Pro Gln Leu Leu Ser Glu Thr Gln Ile Ile Gly Thr Phe 4825 4830 4835 gag ttg cag ggt ccc agc agt gaa ggc gag ggg tct tct ccg ggg 25655 Glu Leu Gln Gly Pro Ser Ser Glu Gly Glu Gly Ser Ser Pro Gly 4840 4845 4850 cag agt ctg aaa ctg acc ccg ggg cta tgg acc tcc gcc tac ctg 25700 Gln Ser Leu Lys Leu Thr Pro Gly Leu Trp Thr Ser Ala Tyr Leu 4855 4860 4865 cgc aag ttc gcc cct gaa gac tac cac ccc tat gag atc agg ttc 25745 Arg Lys Phe Ala Pro Glu Asp Tyr His Pro Tyr Glu Ile Arg Phe 4870 4875 4880 tat gag gac caa tca cag ccg ccc aaa acc gag ctc tca gcc tgc 25790 Tyr Glu Asp Gln Ser Gln Pro Pro Lys Thr Glu Leu Ser Ala Cys 4885 4890 4895 gtc atc act cag ggg gca att ctc gcc caa ttg caa gcc atc caa 25835 Val Ile Thr Gln Gly Ala Ile Leu Ala Gln Leu Gln Ala Ile Gln 4900 4905 4910 aaa tcc cgc caa gaa ttt ctg ctg aaa aag ggg aac ggg gtc tac 25880 Lys Ser Arg Gln Glu Phe Leu Leu Lys Lys Gly Asn Gly Val Tyr 4915 4920 4925 ctt gac ccc cag acc ggt gag gag ctc aac aca agg ttc cct cag 25925 Leu Asp Pro Gln Thr Gly Glu Glu Leu Asn Thr Arg Phe Pro Gln 4930 4935 4940 gat gtc cca gcg ccg agg aag caa gaa gtt gaa ggt gca gct gcc 25970 Asp Val Pro Ala Pro Arg Lys Gln Glu Val Glu Gly Ala Ala Ala 4945 4950 4955 gcc ccc aga gga tat gga gga aga ctg gga cag tca ggc aga gga 26015 Ala Pro Arg Gly Tyr Gly Gly Arg Leu Gly Gln Ser Gly Arg Gly 4960 4965 4970 gga gat gga aga ttg gga cag cca ggc aga gga ggc gga cag cct 26060 Gly Asp Gly Arg Leu Gly Gln Pro Gly Arg Gly Gly Gly Gln Pro 4975 4980 4985 gga gga aga cag ttt gga gga gga aga cga gga ggc aga gga ggt 26105 Gly Gly Arg Gln Phe Gly Gly Gly Arg Arg Gly Gly Arg Gly Gly 4990 4995 5000 gga aga agc aac cgc cgc caa aca gtt gtc ctc ggc agc gga gac 26150 Gly Arg Ser Asn Arg Arg Gln Thr Val Val Leu Gly Ser Gly Asp 5005 5010 5015 aag caa ggt ccc aga cag cag cag cac ggc tac aat ctc cgc tcc 26195 Lys Gln Gly Pro Arg Gln Gln Gln His Gly Tyr Asn Leu Arg Ser 5020 5025 5030 ggg ggg ggc cca gcg gcg tcc caa cag tagatgggac gagaccgggc 26242 Gly Gly Gly Pro Ala Ala Ser Gln Gln 5035 gattcccgaa cccgaccacc gcttccaaga ccggtaagaa ggagcggcag ggatacaagt 26302 cctggcgggg gcataagaat gccatcatct cctgcttgca tgaatgcggg ggcaacatat 26362 ccttcacccg gcgctacctg ctcttccacc acggggtgaa cttcccccgc aatgtcttgc 26422 attactaccg tcacctccac agcccctact acaaccagca agtcccggca gcctcggcag 26482 agaaagacag cagcagcagc agcagcgggg acctccagca gaaaaccagc agcagcagtt 26542 agaaaatcca gtgcagcagg aggaggactg aggatcacag cgaacgagcc agcgcagacc 26602 cgagagctga gaaacaggat ctttccaacc ctctatgcca tcttccagca gagtcggggg 26662 caagagcagg aactgaaagt aaaaaaccga tctctgcgct cgctcacccg aagttgtttg 26722 tatcacaaga gcgaagacca acttcagcgc actctcgagg acgccgaggc tctcttcaac 26782 aagtactgcg cgctgactct taaagagtag cccgcgcccg cgctcgctcg aaaaaggcgg 26842 gaattacgtc acccttggca cctgtccttt gccctcgtc atg agt aaa gaa att 26896 Met Ser Lys Glu Ile 5040 ccc acg cct tac atg tgg agc tat cag ccc caa atg gga ctg gca 26941 Pro Thr Pro Tyr Met Trp Ser Tyr Gln Pro Gln Met Gly Leu Ala 5045 5050 5055 gca ggc gcc tcc cag gac tac tcc acc cgc atg aat tgg ctc agc 26986 Ala Gly Ala Ser Gln Asp Tyr Ser Thr Arg Met Asn Trp Leu Ser 5060 5065 5070 gcc ggc ccc tcg atg atc tca cgg gtt aat gat ata cga gct tac 27031 Ala Gly Pro Ser Met Ile Ser Arg Val Asn Asp Ile Arg Ala Tyr 5075 5080 5085 cga aac cag tta ctc cta gaa cag tca gca ctc acc acc aca ccc 27076 Arg Asn Gln Leu Leu Leu Glu Gln Ser Ala Leu Thr Thr Thr Pro 5090 5095 5100 cgc caa cac ctt aat ccc cgg aat tgg ccc gcc gcc ctg gtg tac 27121 Arg Gln His Leu Asn Pro Arg Asn Trp Pro Ala Ala Leu Val Tyr 5105 5110 5115 cag gaa act ccc gct ccc acc acc gta cta ctt cct cga gac gcc 27166 Gln Glu Thr Pro Ala Pro Thr Thr Val Leu Leu Pro Arg Asp Ala 5120 5125 5130 cag gcc gaa gtt cag atg act aac gca ggt gta cag ctg gcg ggc 27211 Gln Ala Glu Val Gln Met Thr Asn Ala Gly Val Gln Leu Ala Gly 5135 5140 5145 ggt tcc gcc ctg tgt cgt cac cgg cct cag cag agt ata aaa cgc 27256 Gly Ser Ala Leu Cys Arg His Arg Pro Gln Gln Ser Ile Lys Arg 5150 5155 5160 ctg gtg atc aga ggc cga ggt atc cag ctt aac gac gag tcg gtg 27301 Leu Val Ile Arg Gly Arg Gly Ile Gln Leu Asn Asp Glu Ser Val 5165 5170 5175 agc tct tcg ctt ggt ctg cga cca gac gga gtc ttc caa att gcc 27346 Ser Ser Ser Leu Gly Leu Arg Pro Asp Gly Val Phe Gln Ile Ala 5180 5185 5190 ggc tgt ggg aga tct tcc ttc act cct cgt cag gct gtc ctg act 27391 Gly Cys Gly Arg Ser Ser Phe Thr Pro Arg Gln Ala Val Leu Thr 5195 5200 5205 ttg gag agt tcg tcc tcg caa ccc cgc tcg ggc ggc atc ggg act 27436 Leu Glu Ser Ser Ser Ser Gln Pro Arg Ser Gly Gly Ile Gly Thr 5210 5215 5220 ctc cag ttt gtg gag gag ttt act ccc tct gtc tac ttc aac ccc 27481 Leu Gln Phe Val Glu Glu Phe Thr Pro Ser Val Tyr Phe Asn Pro 5225 5230 5235 ttc tcc ggc tct cct ggc cag tac ccg gac gag ttc ata ccg aac 27526 Phe Ser Gly Ser Pro Gly Gln Tyr Pro Asp Glu Phe Ile Pro Asn 5240 5245 5250 ttc gac gca atc agc gag tca gtg gat ggc tat gat tg atg tct ggt 27573 Phe Asp Ala Ile Ser Glu Ser Val Asp Gly Tyr Asp Met Ser Gly 5255 5260 5265 ggc gcg gct gag tta gct cga ctg cga cat cta gac cac tgc cgc 27618 Gly Ala Ala Glu Leu Ala Arg Leu Arg His Leu Asp His Cys Arg 5270 5275 5280 cgc ttt cgc tgt ttc gcc cgg gaa ctc acc gag ttc atc tac ttc 27663 Arg Phe Arg Cys Phe Ala Arg Glu Leu Thr Glu Phe Ile Tyr Phe 5285 5290 5295 gaa ctc ccc gag gag cac cct cag gga ccg gcc cac gga gtg cgg 27708 Glu Leu Pro Glu Glu His Pro Gln Gly Pro Ala His Gly Val Arg 5300 5305 5310 att acc atc gaa ggg gga ata gac tct cgc ctg cat cgg atc ttc 27753 Ile Thr Ile Glu Gly Gly Ile Asp Ser Arg Leu His Arg Ile Phe 5315 5320 5325 tgt cag cgg cca gtg ctg atc gaa cgc gac cag gga act aca aca 27798 Cys Gln Arg Pro Val Leu Ile Glu Arg Asp Gln Gly Thr Thr Thr 5330 5335 5340 gtc tcc atc tac tgc atc tgt aac cac ccc gga ttg cat gaa agc 27843 Val Ser Ile Tyr Cys Ile Cys Asn His Pro Gly Leu His Glu Ser 5345 5350 5355 ctt tgc tgt ctt att tgt gct gag ttt aat aaa aac tgagttcaga 27889 Leu Cys Cys Leu Ile Cys Ala Glu Phe Asn Lys Asn 5360 5365 5370 ccctcctacg gactaccgct tcttcaaccc ggactttaca acaccagcca gaccctccgt 27949 tccagccaga agacccaggc ccttcctctg atccaggact ctaattctac ctccccagca 28009 ccatccccta ctaaccttcc cgaaactaac aacctcggag ctcagctgca acaccgcttc 28069 tccagaagcc tcctttctgc caatactact actcccaaaa ccggaggtga gctccgcggt 28129 ctccctactg acaacccctg ggcggtagca ggttttgtag cgttaggagt agttgcgggt 28189 gggctggtgc ttatcctctg ctacctatac acaccttgct gtgcttattt agtaatattg 28249 tgctgctggt ttaagaa atg ggg gtc gta cta gta gcg ctt gct tta ctt 28299 Met Gly Val Val Leu Val Ala Leu Ala Leu Leu 5375 5380 tcg ctt ttg ggt ctg ggc tct act acg cta aga aat cag cct ttg 28344 Ser Leu Leu Gly Leu Gly Ser Thr Thr Leu Arg Asn Gln Pro Leu 5385 5390 5395 cta tta gat ccc aat gat gtt gat cca tgt ctg gac ttt gat cca 28389 Leu Leu Asp Pro Asn Asp Val Asp Pro Cys Leu Asp Phe Asp Pro 5400 5405 5410 gag aac tgc aca ctc act ttt gca cct gaa aca agt cgc ttc tgt 28434 Glu Asn Cys Thr Leu Thr Phe Ala Pro Glu Thr Ser Arg Phe Cys 5415 5420 5425 gga gtt gtt att agg tgc gga ttt gaa tgc agg ccc att gag att 28479 Gly Val Val Ile Arg Cys Gly Phe Glu Cys Arg Pro Ile Glu Ile 5430 5435 5440 aca cac aat aac aaa act tgg aac aat acc tta ttt acc aca tgg 28524 Thr His Asn Asn Lys Thr Trp Asn Asn Thr Leu Phe Thr Thr Trp 5445 5450 5455 tct cca gga gat cct cag tgg tat act gtc tct gtc cgg ggt cct 28569 Ser Pro Gly Asp Pro Gln Trp Tyr Thr Val Ser Val Arg Gly Pro 5460 5465 5470 gac ggt tcc gtc cgc atg gct aat aac act ttc att ttt gct gaa 28614 Asp Gly Ser Val Arg Met Ala Asn Asn Thr Phe Ile Phe Ala Glu 5475 5480 5485 atg tgc gat atg gcc atg ttc atg agc aga cag tat gac cta tgg 28659 Met Cys Asp Met Ala Met Phe Met Ser Arg Gln Tyr Asp Leu Trp 5490 5495 5500 cct ccc agc aaa gag aac att gtg gca ttc tcc att gct tat tgc 28704 Pro Pro Ser Lys Glu Asn Ile Val Ala Phe Ser Ile Ala Tyr Cys 5505 5510 5515 ttg ggt aca tgc atc atc act gct atc atg tgt gtg agc ata cac 28749 Leu Gly Thr Cys Ile Ile Thr Ala Ile Met Cys Val Ser Ile His 5520 5525 5530 ttg ctt ata gcc att cgc cca aaa aac aat caa gaa aaa gag aaa 28794 Leu Leu Ile Ala Ile Arg Pro Lys Asn Asn Gln Glu Lys Glu Lys 5535 5540 5545 atg ccc tgattataaa tttctattta cagaaaatga cctctgtttc agctctcata 28850 Met Pro tttgctacta ttatggctgt tcaaggacag gctgttcaag gacagacact tattaatgtt 28910 catcctggaa ctaatcatac cttggtggtt cctaataact attcaaatat tgaatggcaa 28970 tggttcacaa acaacgtatg gtatgaacca tgcgaacatt acagcctatt catttgcaat 29030 cataatttaa ctttaatcaa tgtcagcaca atacacaaag gatactatta tagatatgac 29090 aaccacagca ttgatcctac aatatatcta gtacgtgtaa atccaattaa caaacctata 29150 cccaaagctt tctctagaac tacaatacaa aactttaaaa cagcaatttt acttaatttt 29210 aaaaccaaaa atattacagg caatatactt cccactactc ccactgaaaa aaatacacct 29270 aattcaatat ttgaaatcat cattgcactg ttagcagtag gcataacaat catactatgt 29330 atgataattt atgctcactg ttataaaaaa attcaccaca aaaaagaacc actactaagc 29390 ttttaatttc ttttttatac agcc atg att ttc ttc gca act ctt att act 29441 Met Ile Phe Phe Ala Thr Leu Ile Thr 5550 5555 att ggc att gtt caa ggg caa gat atc aca att gga tat gta ggc 29486 Ile Gly Ile Val Gln Gly Gln Asp Ile Thr Ile Gly Tyr Val Gly 5560 5565 5570 aat aat att acc cta tta ggt ccc cca aca gga aca atc cct acc 29531 Asn Asn Ile Thr Leu Leu Gly Pro Pro Thr Gly Thr Ile Pro Thr 5575 5580 5585 tgg tac aaa ata tat gaa aga ggg tgg tgg att aga ccc tgc gac 29576 Trp Tyr Lys Ile Tyr Glu Arg Gly Trp Trp Ile Arg Pro Cys Asp 5590 5595 5600 caa gga ggt agt aaa tac att tgt ggt aga gac ata acc atc acc 29621 Gln Gly Gly Ser Lys Tyr Ile Cys Gly Arg Asp Ile Thr Ile Thr 5605 5610 5615 aat ctt aat aaa aac gat aat ggc tac tat ttt tgc aat aac tat 29666 Asn Leu Asn Lys Asn Asp Asn Gly Tyr Tyr Phe Cys Asn Asn Tyr 5620 5625 5630 gga ggt ggt aaa aag tct tac aca ctt gaa gta aga gac ccc acc 29711 Gly Gly Gly Lys Lys Ser Tyr Thr Leu Glu Val Arg Asp Pro Thr 5635 5640 5645 act tta gca cca cat acc act ttc tcc agc agc acg tct aga aac 29756 Thr Leu Ala Pro His Thr Thr Phe Ser Ser Ser Thr Ser Arg Asn 5650 5655 5660 aca cat gag gca gct tat gcc aga gca atg ctt caa aaa att aat 29801 Thr His Glu Ala Ala Tyr Ala Arg Ala Met Leu Gln Lys Ile Asn 5665 5670 5675 gaa aca ata aat tct aca atc tct cat aat cca gac gaa att ccc 29846 Glu Thr Ile Asn Ser Thr Ile Ser His Asn Pro Asp Glu Ile Pro 5680 5685 5690 aaa tca atg att ggc att att gta gcc gtg gca gtt gga atg gca 29891 Lys Ser Met Ile Gly Ile Ile Val Ala Val Ala Val Gly Met Ala 5695 5700 5705 atc ata ata att tgt atg atc gtc tat gct tgc tgc tat aga aag 29936 Ile Ile Ile Ile Cys Met Ile Val Tyr Ala Cys Cys Tyr Arg Lys 5710 5715 5720 ttt caa gat gaa aaa gga gac cca cta cta agc ttt gat att 29978 Phe Gln Asp Glu Lys Gly Asp Pro Leu Leu Ser Phe Asp Ile 5725 5730 5735 taatttcttt atagaaac atg aaa gga gta ggt atc cta gtt ctt tca act 30029 Met Lys Gly Val Gly Ile Leu Val Leu Ser Thr 5740 5745 tta atc tac tca gtg atc cct atc agc atc aat gtg cag act act 30074 Leu Ile Tyr Ser Val Ile Pro Ile Ser Ile Asn Val Gln Thr Thr 5750 5755 5760 tta aat gaa act gga aac cac tca act acc tca cat aca cct ccc 30119 Leu Asn Glu Thr Gly Asn His Ser Thr Thr Ser His Thr Pro Pro 5765 5770 5775 ccg ctt tct acc cac cct caa tcc aaa gat gcc ata caa cta caa 30164 Pro Leu Ser Thr His Pro Gln Ser Lys Asp Ala Ile Gln Leu Gln 5780 5785 5790 ctc acc atc ctt att gtg att ggg tta act atc ctt gct gtt atc 30209 Leu Thr Ile Leu Ile Val Ile Gly Leu Thr Ile Leu Ala Val Ile 5795 5800 5805 ctt tac ttt atc ttt tgc cgc caa ata ccc aat gta gtt aaa cct 30254 Leu Tyr Phe Ile Phe Cys Arg Gln Ile Pro Asn Val Val Lys Pro 5810 5815 5820 acc aga cgt ccc atc tat cga tca ata atc agc aaa ccc cac atg 30299 Thr Arg Arg Pro Ile Tyr Arg Ser Ile Ile Ser Lys Pro His Met 5825 5830 5835 gct cta aat gaa att taatctttct cttcacagta tggtgatcaa ct atg atc 30352 Ala Leu Asn Glu Ile Met Ile 5840 5845 cct aga aat ttc ttc ttc acc ata ctt atc tgc gct ttc aat gtc 30397 Pro Arg Asn Phe Phe Phe Thr Ile Leu Ile Cys Ala Phe Asn Val 5850 5855 5860 tgt gct aca ttc gcc aca gtc gcc aat gtg aca cca gat tgt ata 30442 Cys Ala Thr Phe Ala Thr Val Ala Asn Val Thr Pro Asp Cys Ile 5865 5870 5875 ggg gca ttt gct tcc tac gta cta ttt gcc ttc att acc tgc atc 30487 Gly Ala Phe Ala Ser Tyr Val Leu Phe Ala Phe Ile Thr Cys Ile 5880 5885 5890 tgc gtt tgt agc ata gtc tgc ctg gtt atc aac ttc ttt caa cta 30532 Cys Val Cys Ser Ile Val Cys Leu Val Ile Asn Phe Phe Gln Leu 5895 5900 5905 gta gac tgg gtt ttt gta cgc att gcc tac cta cga cat cac cct 30577 Val Asp Trp Val Phe Val Arg Ile Ala Tyr Leu Arg His His Pro 5910 5915 5920 gaa tac cgc aac caa aat gtt gca gca att ctt agg ctc att 30619 Glu Tyr Arg Asn Gln Asn Val Ala Ala Ile Leu Arg Leu Ile 5925 5930 taaaaccatg caaactctgc tactgcttct gctagttata caccaatgtg cctcaaaccc 30679 cacaagcccc acaaaattag atctaagaaa atgtaaattt caagaaccat ggaaattcct 30739 tgattgctat catgaaacat ctgatttccc cacatactgg attacaatca ttggggttgt 30799 taatctagtc tcttgcacac tattctcttt ccttgtttac cacttatttg attttggatg 30859 gaacgccctt aatgcactca cttacccaca agaaccagag gaacatatac cactacagaa 30919 catacaacca ttagcactag aatatgaaaa tgagccacag cctccactac tccctgccat 30979 tagctacttc aacctaaccg gtggag atg act gac cca cac gcc gct gct 31029 Met Thr Asp Pro His Ala Ala Ala 5935 5940 gag gaa cta ctt gat atg gac ggc cgt gcc tcc gaa cag cgc ctc 31074 Glu Glu Leu Leu Asp Met Asp Gly Arg Ala Ser Glu Gln Arg Leu 5945 5950 5955 gct caa cta cgc att cgc cag cag cag gaa cgt gcc gcc aag gag 31119 Ala Gln Leu Arg Ile Arg Gln Gln Gln Glu Arg Ala Ala Lys Glu 5960 5965 5970 ctc agg gat gcc att gag att cac cag tgc aaa aaa ggc ata ttc 31164 Leu Arg Asp Ala Ile Glu Ile His Gln Cys Lys Lys Gly Ile Phe 5975 5980 5985 tgc ttg gta aaa caa gcc aag atc tcc tac gag atc acc gct aac 31209 Cys Leu Val Lys Gln Ala Lys Ile Ser Tyr Glu Ile Thr Ala Asn 5990 5995 6000 gac cac cgc ctc tca tat gag ctt ggc ccg cag cgt cag aaa ttc 31254 Asp His Arg Leu Ser Tyr Glu Leu Gly Pro Gln Arg Gln Lys Phe 6005 6010 6015 act tgc atg gtg gga atc aac ccc ata gtc atc acc cag caa gct 31299 Thr Cys Met Val Gly Ile Asn Pro Ile Val Ile Thr Gln Gln Ala 6020 6025 6030 gga gat acc aag ggt tgc atc cat tgt tcc tgt gaa tcc acc gag 31344 Gly Asp Thr Lys Gly Cys Ile His Cys Ser Cys Glu Ser Thr Glu 6035 6040 6045 tgc atc tac acc ctg ctg aag acc ctc tgc ggc ctt cga gac ctc 31389 Cys Ile Tyr Thr Leu Leu Lys Thr Leu Cys Gly Leu Arg Asp Leu 6050 6055 6060 cta ccc atg aac taatcaacaa cccctaccct cttcccatta aaatccaatt 31441 Leu Pro Met Asn 6065 aataaaattc acttacttaa aatcagaaac aaagtttttg tccaagttgt tttcaaccag 31501 cacctcactt ccctcttccc aactctggta ctctaagcct cggcgggtgg catacttcct 31561 ccacactttg aaagggatgt caaattttag ttcttctttt cccacaatct tcatttcttt 31621 attcccag atg gcc aaa cga gct cgt cta agc agc tcc ttc aac ccg 31668 Met Ala Lys Arg Ala Arg Leu Ser Ser Ser Phe Asn Pro 6070 6075 gtc tac ccc tat gaa gat gaa agc agt tca caa cac ccc ttt ata 31713 Val Tyr Pro Tyr Glu Asp Glu Ser Ser Ser Gln His Pro Phe Ile 6080 6085 6090 aac cct ggt ttc att tcc cct aat ggg ttt aca caa agt cca gac 31758 Asn Pro Gly Phe Ile Ser Pro Asn Gly Phe Thr Gln Ser Pro Asp 6095 6100 6105 gga gct ctt aca ctc aag tgt gtt gcc cct ctt act acc acc agt 31803 Gly Ala Leu Thr Leu Lys Cys Val Ala Pro Leu Thr Thr Thr Ser 6110 6115 6120 ggc tcc ctg gat att aaa gta gga ggg ggg ctt aag gta gac tcc 31848 Gly Ser Leu Asp Ile Lys Val Gly Gly Gly Leu Lys Val Asp Ser 6125 6130 6135 act gat ggg tcc tta gaa gaa aac ata agc act aca gca cca ctt 31893 Thr Asp Gly Ser Leu Glu Glu Asn Ile Ser Thr Thr Ala Pro Leu 6140 6145 6150 aac aaa tct aat cat tcc ata gga tta gca gtg gga aat gga tta 31938 Asn Lys Ser Asn His Ser Ile Gly Leu Ala Val Gly Asn Gly Leu 6155 6160 6165 caa aca aat gaa agc aaa cta tgt gcc aaa tta gga gag gaa ctt 31983 Gln Thr Asn Glu Ser Lys Leu Cys Ala Lys Leu Gly Glu Glu Leu 6170 6175 6180 acc ttt gat tct tcc aat gcc att aca ata aaa aat aac act tta 32028 Thr Phe Asp Ser Ser Asn Ala Ile Thr Ile Lys Asn Asn Thr Leu 6185 6190 6195 tgg aca gga gca aaa cca agt act aac tgt aaa att caa gaa gat 32073 Trp Thr Gly Ala Lys Pro Ser Thr Asn Cys Lys Ile Gln Glu Asp 6200 6205 6210 gca gat gcc cta gac tgc aag cta act cta gtc ctt gta aaa aat 32118 Ala Asp Ala Leu Asp Cys Lys Leu Thr Leu Val Leu Val Lys Asn 6215 6220 6225 gga gga cta gta aat gca tat gtg tca tta ata gga gac tca gac 32163 Gly Gly Leu Val Asn Ala Tyr Val Ser Leu Ile Gly Asp Ser Asp 6230 6235 6240 tat gtt aat aca cta ttc act aaa aag act gca tca atc agc gta 32208 Tyr Val Asn Thr Leu Phe Thr Lys Lys Thr Ala Ser Ile Ser Val 6245 6250 6255 gaa ctt gcc ttt gat agc tcc ggt caa ata ctt act agc cta tct 32253 Glu Leu Ala Phe Asp Ser Ser Gly Gln Ile Leu Thr Ser Leu Ser 6260 6265 6270 tct cta aaa act agc ctc aac ttt aaa cac aac caa gac atg gcc 32298 Ser Leu Lys Thr Ser Leu Asn Phe Lys His Asn Gln Asp Met Ala 6275 6280 6285 act gaa act atc agt gcc aaa ggc ttc atg cct agt acc act gct 32343 Thr Glu Thr Ile Ser Ala Lys Gly Phe Met Pro Ser Thr Thr Ala 6290 6295 6300 tat ccc ttt aac acc cag gct act tct tct aga gac aat gaa gat 32388 Tyr Pro Phe Asn Thr Gln Ala Thr Ser Ser Arg Asp Asn Glu Asp 6305 6310 6315 tac att ttt ggt aaa tgt tac tac aga gcc tca tat gga gct cta 32433 Tyr Ile Phe Gly Lys Cys Tyr Tyr Arg Ala Ser Tyr Gly Ala Leu 6320 6325 6330 tac act ttg gat gtt act gta ata ctc aac aga cgt atg acc gct 32478 Tyr Thr Leu Asp Val Thr Val Ile Leu Asn Arg Arg Met Thr Ala 6335 6340 6345 gct gga atg gct tat gca atg aac ttt acg tgg ctt ctt gac gcg 32523 Ala Gly Met Ala Tyr Ala Met Asn Phe Thr Trp Leu Leu Asp Ala 6350 6355 6360 aca gat gcc cca gaa aat acc aca acc acc ttg gtc acc tcc ccc 32568 Thr Asp Ala Pro Glu Asn Thr Thr Thr Thr Leu Val Thr Ser Pro 6365 6370 6375 ttc tcc ttt tcc tat att aga gaa gat gac tgacaacaaa ataaagttca 32618 Phe Ser Phe Ser Tyr Ile Arg Glu Asp Asp 6380 6385 actttttatt gaaaatcagt ttacaggata cgagtagtta ttttgcctcc cccttcccat 32678 ttcatagaat acaccaatct ctccccacgc acagctttaa acatttggat tccatttgag 32738 atagtcatgg atttagattc cacattccac acagtttcag agctacataa tcttggatca 32798 gtgatagaga taaatccatc ggggcaatcc ttcaaggtaa tttcacagtc cagttgctgt 32858 ggctgcggct ccggagtctg gatcagagtc atctggaaga agaacgatgg gagtcataat 32918 ccgagaacgg gatcgggcgg ttgtgtctca tcaaaccccg aagcagtcgc tgtctgcgcc 32978 gctccgtgcg actgctgctg atgggatcgg ggtccacagt ctctcgaagc atgattctaa 33038 tagccctcaa cattaacatc ctggtgcgat gcgcacagca gcgcatcctg atctcactta 33098 aatcacagca gtaggtacaa cacaacacca caatattgtt taacaggcca taattaaagg 33158 cgctccagcc aaaactcatt tcaggaataa tttgccccgc gtggccatcg taccaaatcc 33218 tgatgaaaat tagatggcgc cccctccaga atacactgcc cacatacatg atctccttag 33278 gcatatgcat attcacaatc tctcggtacc atggacagcg ctggttaatc atgcagcccc 33338 gaataacctt ccggaaccaa atggccagca atgcgccccc agcaatacat tgaagagaac 33398 cctgtcgatt acagtgacaa tggagaaccc acttctctcg cccatggatc acttgggaat 33458 aaaatatatc tattgtggca caacacagac ataaatgcat acatcttctc atcaccctta 33518 actcttcagg ggttaaaaac atatcccagg gaataggaag ctcttgcaaa acagtaaagg 33578 tggcagaaca aggcagaccg cgaacataac ttacactatg catggtcaag gtattgcaat 33638 ctggtaacag cggatgctct tcagtcatag aagctctggt ttcactttcc tcacagcgtg 33698 gtaaaggggc cctcagttga ggttccctgg tgtaaggatg gtgtctggcg cacgatgtcg 33758 agcgtgcacg cgacctcgtt gtaatggagc tgcttcctga cattctcgta ttttgcatgg 33818 cagaacctag ccttggcaca acacacttct cttcgccttc tatcccgtcg cctagcacgt 33878 tcagtatggt aattgaagta cagccattcc cgtagattgg tcaaaagttc ctcggcttca 33938 gttgtcataa aaactccatc atatcttact gctctgataa aatcattcac tgtagaatgg 33998 gcaatgccca gccaggcaat gcaattagct tgtgtttcaa ccaaaggagg gggaggaaga 34058 catggaagaa ccataattaa tttttattcc agacgatccc gcagtatttc tacatggaga 34118 tcacgaagat ggcacctctc gcccccactg tgttgatgaa aaatgacagc taggtcaaac 34178 ataatgcgat tttccaggtg ctcaacggtg gcttcaagca aagcctccaa acgtacatcc 34238 aaaaacaaaa gaacagcaaa agcaggagca ttttctaatt cctcaatcat catattacat 34298 tcctgtacca ttcccaaata attttcatct ttccatcctt gaattattcg tgttatttca 34358 tctggtaaat ccaatccaca catgagaaat agctcccgaa gggcgccctc caccggcatt 34418 cttaagcaca ccctcatagt gaaaaaatat cgtgctcctc tgtcacctgc agcaaattaa 34478 gaatggcaac atcatactgg atgccactgg ctctaagttc ttctctaagt tccagttgta 34538 aaaactcttg catatcatcg ccaaactgct tggccatagg tcctccagga ataagagctg 34598 gggacgctac agtgcagaac aagcgcatgc caccccaatt gcctccagca aaagttaggt 34658 tgcaatatgc atactgagaa cctccagtga tatcatccag tgtactggaa agataatcag 34718 gcagagcttc tcgtatacaa ttaataatag aaaagtctgc cagatgaaca tttaaagcct 34778 gtgggatgca gatgcaataa gttatcgcgc tgcgctccaa cattgttagt atggttagtc 34838 tgtaaaaaca aaaaacaaaa ttacatcacg ctgtactggc gaacgggtgg ataaatcact 34898 ctctccaaca ccaggcaggc tacagggtct ccagcgcgac cctcgtaaaa cctgtcagta 34958 tgattaaaaa gcatcaccga aagagattgt tgatggccag catatattat ttgcgatgaa 35018 gcatacaaac cagaagtgtt agtatcagtt aaagaaaaaa atcggccaag atagcatctc 35078 ggaacgatta tgctcaatct caaatgcagc aaagcgacac ctcgcggatg caaagtaaaa 35138 tccacaggag cataaaaaat gtaattattc ccctcttgca caggcagcct agctcccggc 35198 ccctccagga tcacatacaa agcctcagca gccatagctt accgcgcaaa tcaggcacag 35258 cagtcagata acgagaaagc tgtgaactga ctgcccagcc tgtgcgcaat atatagagaa 35318 cccttacact gacgtaattg gacaaagtct aaaaaatccc gccaaaaacc agcacacgcc 35378 cagaactgtg tcacccgcta aaaaataatt ttcacttcct cgttccgtga atgacgtcag 35438 ttcccctttc ccacgagccg tcacttccgg gcatcttgca acgtcacctc cccgcgccgg 35498 cccgcccctt ttgaccgttg aacccgctgg ccaatcccct tccgccctcc attttcaaaa 35558 gctcatttgc atgttggcac cgttccattt ataaggtata ttattgatga tg 35610 <210> 2 <211> 495 <212> PRT <213> Simian adenovirus 28 <400> 2 Met Asp Pro Thr Asn Pro Leu Gln Gln Gly Ile Arg Leu Gly Phe His 1 5 10 15 Ser Ser Ser Phe Val Glu Asn Met Glu Gly Pro Gln Ala Glu Asp Asn 20 25 30 Leu Arg Leu Leu Ala Ser Ala Ala Ser Gly Arg Ser Gly Asp Pro Glu 35 40 45 Thr Pro Thr Gly His Ala Ser Gly Phe Gly Gly Gly Ala Ala Gly Gly 50 55 60 Gln Pro Glu Ser Arg Pro Gly Pro Ser Gly Gly Gly Gly Gly Gly Val 65 70 75 80 Ala Asp Leu Phe Pro Glu Leu Arg Arg Val Leu Thr Arg Ser Thr Ser 85 90 95 Ser Gly Gln Asp Arg Gly Ile Lys Arg Glu Arg Asn Ala Ser Gly His 100 105 110 Asn Ser Arg Thr Glu Leu Ala Leu Ser Leu Met Ser Arg Ser Arg Pro 115 120 125 Glu Thr Ile Trp Trp His Glu Val Gln Ser Glu Gly Arg Asp Glu Val 130 135 140 Ser Ile Leu Gln Glu Lys Tyr Ser Leu Glu Gln Ile Lys Thr Cys Trp 145 150 155 160 Leu Glu Pro Glu Asp Asp Trp Glu Val Ala Ile Arg Asn Tyr Ala Lys 165 170 175 Ile Ser Leu Arg Pro Asp Lys Gln Tyr Arg Ile Thr Lys Lys Ile Asn 180 185 190 Ile Arg Asn Ala Cys Tyr Ile Ser Gly Asn Gly Ala Glu Val Ile Ile 195 200 205 Asp Thr Pro Asp Lys Thr Ala Phe Arg Cys Cys Met Met Gly Met Trp 210 215 220 Pro Gly Val Ala Gly Met Glu Ala Val Thr Leu Met Asn Ile Arg Phe 225 230 235 240 Arg Gly Asp Gly Tyr Asn Gly Ile Val Phe Met Ala Asn Thr Lys Leu 245 250 255 Ile Leu His Gly Cys Ser Phe Phe Gly Phe Asn Asn Thr Cys Val Glu 260 265 270 Ala Trp Gly Gln Val Ser Val Arg Gly Cys Ser Phe Tyr Ala Cys Trp 275 280 285 Ile Ala Leu Ser Gly Arg Thr Lys Ser Gln Leu Ser Val Lys Lys Cys 290 295 300 Met Phe Glu Arg Cys Asn Leu Gly Ile Leu Asn Glu Gly Glu Ala Arg 305 310 315 320 Val Arg His Cys Ala Ala Thr Glu Thr Gly Cys Phe Ile Leu Ile Lys 325 330 335 Gly Asn Ala Ser Val Lys His Asn Met Ile Cys Gly Pro Ser Asp Glu 340 345 350 Arg Pro Tyr Gln Met Leu Thr Cys Ala Gly Gly His Cys Asn Met Leu 355 360 365 Ala Thr Val His Ile Val Ser His Ala Arg Lys Lys Trp Pro Val Phe 370 375 380 Glu His Asn Val Met Thr Lys Cys Thr Met His Ile Gly Gly Arg Arg 385 390 395 400 Gly Met Phe Met Pro Tyr Gln Cys Asn Met Asn His Val Lys Val Met 405 410 415 Leu Glu Pro Asp Ala Phe Ser Arg Met Ser Leu Thr Gly Ile Phe Asp 420 425 430 Met Asn Val Gln Leu Trp Lys Ile Leu Arg Tyr Asp Glu Thr Lys Ser 435 440 445 Arg Val Arg Ala Cys Glu Cys Gly Gly Lys His Ala Arg Phe Gln Pro 450 455 460 Val Cys Val Asp Val Thr Glu Asp Leu Arg Pro Asp His Leu Val Leu 465 470 475 480 Ala Cys Thr Gly Ala Glu Phe Gly Ser Ser Gly Glu Glu Thr Asp 485 490 495 <210> 3 <211> 138 <212> PRT <213> Simian adenovirus 28 <400> 3 Met Ser Gly Ser Ala Ser Phe Glu Gly Gly Val Phe Ser Pro Tyr Leu 1 5 10 15 Thr Gly Arg Leu Pro Pro Trp Ala Gly Val Arg Gln Asn Val Met Gly 20 25 30 Ser Thr Val Asp Gly Arg Pro Val Gln Pro Ala Asn Ser Ser Thr Leu 35 40 45 Thr Tyr Ala Thr Leu Ser Ser Ser Ser Leu Asp Ala Ala Ala Ala Ala 50 55 60 Ala Ala Ser Ala Ala Ala Asn Thr Val Leu Gly Met Gly Tyr Tyr Gly 65 70 75 80 Ser Ile Val Ala Asn Ser Ser Ser Ser Asn Asn Pro Ser Thr Leu Ala 85 90 95 Glu Asp Lys Leu Leu Val Leu Leu Ala Gln Leu Glu Ala Leu Thr Gln 100 105 110 Arg Leu Gly Glu Leu Ser Gln Gln Val Ala Gln Leu Arg Glu Gln Thr 115 120 125 Glu Ser Ala Val Ala Thr Ala Lys Ser Lys 130 135 <210> 4 <211> 389 <212> PRT <213> Simian adenovirus 28 <400> 4 Met His Pro Val Leu Arg Gln Met Arg Pro Gln Gln Gln Val Pro Ser 1 5 10 15 Gln Gln Gln Gln Gln Pro Gln Lys Ala Leu Pro Ala Pro Ala Pro Ala 20 25 30 Thr Thr Ala Val Ala Ala Val Cys Gly Ala Gly Gln Pro Ala Tyr Asp 35 40 45 Leu Asp Leu Glu Glu Gly Glu Gly Leu Ala Arg Leu Gly Ala Pro Ser 50 55 60 Pro Glu Arg His Pro Arg Val Gln Leu Lys Lys Asp Ser Arg Glu Ala 65 70 75 80 Tyr Val Pro Gln His Asn Leu Phe Arg Asp Arg Ser Gly Glu Glu Pro 85 90 95 Glu Glu Met Arg Ala Ser Arg Phe Asn Ala Gly Arg Glu Leu Arg His 100 105 110 Gly Leu Asp Arg Arg Arg Val Leu Arg Asp Glu Asp Phe Glu Val Asp 115 120 125 Glu Val Thr Gly Ile Ser Pro Ala Arg Ala His Val Ala Ala Ala Asn 130 135 140 Leu Val Ser Ala Tyr Glu Gln Thr Val Lys Glu Glu Arg Asn Phe Gln 145 150 155 160 Lys Ser Phe Asn Asn His Val Arg Thr Leu Ile Ala Arg Glu Glu Val 165 170 175 Thr Leu Gly Leu Met His Leu Trp Asp Leu Met Glu Ala Ile Thr Gln 180 185 190 Asn Pro Thr Ser Lys Pro Leu Thr Ala Gln Leu Phe Leu Val Val Gln 195 200 205 His Ser Arg Asp Asn Glu Ala Phe Arg Glu Ala Leu Leu Asn Ile Thr 210 215 220 Glu Pro Glu Gly Arg Trp Leu Tyr Asp Leu Ile Asn Ile Leu Gln Ser 225 230 235 240 Ile Ile Val Gln Glu Arg Ser Leu Gly Leu Ala Glu Lys Val Ala Ala 245 250 255 Ile Asn Tyr Ser Val Leu Ser Leu Gly Lys Tyr Tyr Ala Arg Lys Ile 260 265 270 Tyr Lys Thr Pro Tyr Val Pro Ile Asp Lys Glu Val Lys Ile Asp Gly 275 280 285 Phe Tyr Met Arg Met Thr Leu Lys Val Leu Thr Leu Ser Asp Asp Leu 290 295 300 Gly Val Tyr Arg Asn Asp Arg Met His Arg Ala Val Ser Ala Ser Arg 305 310 315 320 Arg Arg Glu Leu Ser Asp Arg Glu Leu Met His Ser Leu Gln Arg Ala 325 330 335 Leu Thr Gly Ala Gly Thr Asp Gly Glu Asn Tyr Phe Asp Met Gly Ala 340 345 350 Asp Leu Gln Trp Gln Pro Ser Arg Arg Thr Leu Asp Ala Ala Gly Cys 355 360 365 Glu Leu Pro Tyr Val Glu Glu Val Asp Glu Gly Glu Glu Glu Glu Gly 370 375 380 Glu Tyr Leu Glu Asp 385 <210> 5 <211> 587 <212> PRT <213> Simian adenovirus 28 <400> 5 Met Glu Gln Gln Ala Pro Asp Pro Ala Met Arg Ala Ala Leu Gln Ser 1 5 10 15 Gln Pro Ser Gly Ile Asn Ser Ser Asp Asp Trp Thr Gln Ala Met Gln 20 25 30 Arg Ile Met Ala Leu Thr Thr Arg Asn Pro Glu Ala Phe Arg Gln Gln 35 40 45 Pro Gln Ala Asn Arg Leu Ser Ala Ile Leu Glu Ala Val Val Pro Ser 50 55 60 Arg Ser Asn Pro Thr His Glu Lys Val Leu Ala Ile Val Asn Ala Leu 65 70 75 80 Val Glu Asn Lys Ala Ile Arg Pro Asp Glu Ala Gly Leu Val Tyr Asn 85 90 95 Ala Leu Leu Glu Arg Val Ala Arg Tyr Asn Ser Ser Asn Val Gln Thr 100 105 110 Asn Leu Asp Arg Met Val Thr Asp Val Arg Glu Ala Val Ser Gln Arg 115 120 125 Glu Arg Phe Gln Arg Asp Ala Asn Leu Gly Ser Leu Val Ala Leu Asn 130 135 140 Ala Phe Leu Ser Thr Gln Pro Ala Asn Val Pro Arg Gly Gln Gln Asp 145 150 155 160 Tyr Thr Asn Phe Leu Ser Ala Leu Arg Leu Met Val Thr Glu Val Pro 165 170 175 Gln Ser Glu Val Tyr Gln Ser Gly Pro Asp Tyr Phe Phe Gln Thr Ser 180 185 190 Arg Gln Gly Leu Gln Thr Val Asn Leu Ser Gln Ala Phe Lys Asn Leu 195 200 205 Lys Gly Leu Trp Gly Val His Ala Pro Val Gly Asp Arg Ala Thr Val 210 215 220 Ser Ser Leu Leu Thr Pro Asn Ser Arg Leu Leu Leu Leu Leu Val Ala 225 230 235 240 Pro Phe Thr Asp Ser Gly Ser Ile Asp Arg Asn Ser Tyr Leu Gly Tyr 245 250 255 Leu Leu Asn Leu Tyr Arg Glu Ala Ile Gly Gln Ser Gln Val Asp Glu 260 265 270 Gln Thr Tyr Gln Glu Ile Thr Gln Val Ser Arg Ala Leu Gly Gln Glu 275 280 285 Asp Thr Gly Ser Leu Glu Ala Thr Leu Asn Phe Leu Leu Thr Asn Arg 290 295 300 Ser Gln Lys Ile Pro Pro Gln Tyr Ala Leu Thr Ala Glu Glu Glu Arg 305 310 315 320 Ile Leu Arg Tyr Val Gln Gln Ser Val Gly Leu Phe Leu Met Gln Glu 325 330 335 Gly Ala Thr Pro Ser Ala Ala Leu Asp Met Thr Ala Arg Asn Met Glu 340 345 350 Pro Ser Met Tyr Ala Ser Asn Arg Pro Phe Ile Asn Lys Leu Leu Asp 355 360 365 Tyr Leu His Arg Ala Ala Ala Met Asn Ser Asp Tyr Phe Thr Asn Ala 370 375 380 Ile Leu Asn Pro His Trp Leu Pro Pro Pro Gly Phe Tyr Thr Gly Glu 385 390 395 400 Tyr Asp Met Pro Asp Pro Asn Asp Gly Phe Leu Trp Asp Asp Val Asp 405 410 415 Ser Ser Ile Phe Ser Pro Pro Pro Gly Tyr Asn Thr Trp Lys Lys Glu 420 425 430 Gly Gly Asp Arg Arg His Ser Ser Val Ser Leu Ser Gly Ser Arg Gly 435 440 445 Ala Ala Ala Ala Val Pro Glu Ala Ala Ser Pro Phe Pro Ser Leu Pro 450 455 460 Phe Ser Leu Asn Ser Val Arg Ser Ser Glu Leu Gly Arg Ile Thr Arg 465 470 475 480 Pro Arg Leu Met Gly Glu Asp Glu Tyr Leu Asn Asp Ser Leu Leu Arg 485 490 495 Pro Glu Arg Glu Lys Asn Phe Pro Asn Asn Gly Ile Glu Ser Leu Val 500 505 510 Asp Lys Met Ser Arg Trp Lys Thr Tyr Ala Gln Asp His Lys Asp Glu 515 520 525 Pro Arg Ile Leu Gly Ala Ala Ser Gly Thr Thr Arg Arg Arg Gln Arg 530 535 540 His Asp Arg Gln Arg Gly Leu Val Trp Asp Asp Glu Asp Ser Ala Asp 545 550 555 560 Asp Ser Ser Val Leu Asp Leu Gly Gly Arg Gly Gly Gly Asn Pro Phe 565 570 575 Ala His Leu Arg Pro His Phe Gly Arg Met Leu 580 585 <210> 6 <211> 582 <212> PRT <213> Simian adenovirus 28 <400> 6 Met Met Arg Arg Ala Val Leu Gly Gly Ala Val Val Tyr Pro Glu Gly 1 5 10 15 Pro Pro Pro Ser Tyr Glu Ser Val Met Gln Gln Gln Ala Ala Ala Val 20 25 30 Met Gln Pro Ser Leu Glu Ala Pro Phe Val Pro Pro Arg Tyr Leu Ala 35 40 45 Pro Thr Glu Gly Arg Asn Ser Ile Arg Tyr Ser Glu Leu Ala Pro Gln 50 55 60 Tyr Asp Thr Thr Arg Leu Tyr Leu Val Asp Asn Lys Ser Ala Asp Ile 65 70 75 80 Ala Ser Leu Asn Tyr Gln Asn Asp His Ser Asn Phe Leu Thr Thr Val 85 90 95 Val Gln Asn Asn Asp Phe Thr Pro Thr Glu Ala Ser Thr Gln Thr Ile 100 105 110 Asn Phe Asp Glu Arg Ser Arg Trp Gly Gly Gln Leu Lys Thr Ile Met 115 120 125 His Thr Asn Met Pro Asn Val Asn Glu Tyr Met Phe Ser Asn Lys Phe 130 135 140 Lys Ala Arg Val Met Val Ser Arg Lys Lys Pro Glu Gly Tyr Thr Gly 145 150 155 160 Asp Lys Asn Asp Thr Ser Gln Asp Ile Leu Glu Tyr Glu Trp Phe Glu 165 170 175 Phe Thr Leu Pro Glu Gly Asn Phe Ser Ala Thr Met Thr Ile Asp Leu 180 185 190 Met Asn Asn Ala Ile Ile Asp Asn Tyr Leu Ala Val Gly Arg Gln Asn 195 200 205 Gly Val Leu Glu Ser Asp Ile Gly Val Lys Phe Asp Thr Arg Asn Phe 210 215 220 Arg Leu Gly Trp Asp Pro Ile Thr Lys Leu Val Met Pro Gly Val Tyr 225 230 235 240 Thr Tyr Glu Ala Phe His Pro Asp Ile Val Leu Leu Pro Gly Cys Gly 245 250 255 Val Asp Phe Thr Glu Ser Arg Leu Ser Asn Leu Leu Gly Ile Arg Lys 260 265 270 Arg His Pro Phe Gln Glu Gly Phe Lys Ile Met Tyr Glu Asp Leu Glu 275 280 285 Gly Gly Asn Ile Pro Ala Leu Leu Asp Val Asp Ala Tyr Glu Lys Ser 290 295 300 Lys Lys Glu Asn Thr Asp Thr Thr Thr Thr Thr Thr Val Thr Thr Thr 305 310 315 320 Glu Val Ala Thr Val Ala Arg His Val Ala Glu Val Thr Thr Glu Ala 325 330 335 Ala Thr Val Val Ala Val Asp Pro Ile Val Glu Glu Asn Asn Asn Thr 340 345 350 Val Arg Gly Asp Asn Ile His Thr Ala Asn Glu Met Lys Ala Ala Ala 355 360 365 Asp Asp Thr Thr Val Val Val Val Pro Gly Ala Val Val Thr Glu Thr 370 375 380 Lys Thr Lys Thr Leu Thr Ile Gln Pro Leu Glu Lys Asp Thr Lys Glu 385 390 395 400 Arg Ser Tyr Asn Val Ile Ser Gly Thr Asn Asp Thr Ala Tyr Arg Ser 405 410 415 Trp Tyr Leu Ala Tyr Asn Tyr Gly Asp Pro Glu Lys Gly Val Arg Ser 420 425 430 Trp Thr Leu Leu Thr Thr Ser Asp Val Thr Cys Gly Ala Glu Gln Val 435 440 445 Tyr Trp Ser Leu Pro Asp Met Met Gln Asp Pro Val Thr Phe Arg Ser 450 455 460 Thr Arg Gln Val Ser Asn Tyr Pro Val Val Gly Ala Glu Leu Met Pro 465 470 475 480 Val Phe Ser Lys Ser Phe Tyr Asn Glu Gln Ala Val Tyr Ser Gln Gln 485 490 495 Leu Arg Gln Thr Thr Ser Leu Thr His Ile Phe Asp Arg Phe Pro Glu 500 505 510 Asn Gln Ile Leu Ile Arg Pro Pro Ala Pro Thr Ile Thr Thr Val Ser 515 520 525 Glu Asn Val Pro Ala Leu Thr Asp His Gly Thr Leu Pro Leu Arg Ser 530 535 540 Ser Ile Arg Gly Val Gln Arg Val Thr Val Thr Asp Ala Arg Arg Arg 545 550 555 560 Thr Cys Pro Tyr Val Tyr Lys Ala Leu Gly Ile Val Ala Pro Arg Val 565 570 575 Leu Ser Ser Arg Thr Phe 580 <210> 7 <211> 192 <212> PRT <213> Simian adenovirus 28 <400> 7 Met Ser Ile Leu Ile Ser Pro Ser Asn Asn Thr Gly Trp Gly Leu Arg 1 5 10 15 Thr Pro Thr Arg Met Tyr Gly Gly Ala Arg Lys Arg Ser Thr Gln His 20 25 30 Pro Val Arg Val Arg Gly His Phe Arg Ala Pro Trp Gly Ala Leu Lys 35 40 45 Gly Arg Thr Arg Thr Arg Thr Thr Val Asp Asp Val Ile Asp Gln Val 50 55 60 Val Ala Asp Ala Arg Asn Tyr Thr Pro Ala Ala Pro Ala Ser Thr Val 65 70 75 80 Asp Ala Val Ile Asp Ser Val Val Ala Asp Ala Arg Glu Tyr Ala Arg 85 90 95 Arg Lys Ser Arg Arg Arg Arg Ile Ala Arg Arg His Arg Ala Thr Pro 100 105 110 Ala Met Arg Ala Ala Arg Ala Leu Leu Arg Arg Ala Lys Arg Val Gly 115 120 125 Arg Arg Ala Met Leu Arg Ala Ala Arg Arg Ala Ala Ser Gly Ala Ser 130 135 140 Ala Gly Arg Ser Arg Arg Arg Ala Ala Thr Ala Ala Ala Ala Ala Ile 145 150 155 160 Ala Asn Met Ala Gln Pro Arg Arg Gly Asn Val Tyr Trp Val Arg Asp 165 170 175 Ala Thr Thr Gly Gln Arg Val Pro Val Arg Thr Arg Pro Pro Arg Thr 180 185 190 <210> 8 <211> 350 <212> PRT <213> Simian adenovirus 28 <400> 8 Met Ser Lys Arg Lys Tyr Lys Glu Glu Met Leu Gln Val Ile Ala Pro 1 5 10 15 Glu Ile Tyr Gly Pro Pro Val Lys Asp Glu Lys Lys Pro Arg Lys Ile 20 25 30 Lys Arg Val Lys Lys Asp Lys Lys Glu Glu Asp Gly Asp Asp Gly Leu 35 40 45 Val Glu Phe Val Arg Glu Phe Ala Pro Arg Arg Arg Val Gln Trp Arg 50 55 60 Gly Arg Lys Val Arg Pro Val Leu Arg Pro Gly Thr Thr Val Val Phe 65 70 75 80 Thr Pro Gly Glu Arg Ser Ser Thr Thr Phe Lys Arg Ser Tyr Asp Glu 85 90 95 Val Tyr Gly Asp Asp Asp Ile Leu Glu Gln Ala Ala Asp Arg Leu Gly 100 105 110 Glu Phe Ala Tyr Gly Lys Arg Ser Arg Ser Ser Pro Lys Asp Glu Ala 115 120 125 Val Ser Ile Pro Leu Asp His Gly Asn Pro Thr Pro Ser Leu Lys Pro 130 135 140 Val Thr Leu Gln Gln Val Leu Pro Val Pro Ala Arg Arg Gly Val Lys 145 150 155 160 Arg Glu Gly Glu Asp Leu Tyr Pro Thr Met Gln Leu Met Val Pro Lys 165 170 175 Arg Gln Lys Leu Glu Asp Val Leu Glu Lys Met Lys Val Asp Pro Asp 180 185 190 Ile Gln Pro Glu Val Lys Val Arg Pro Ile Lys Gln Val Ala Pro Gly 195 200 205 Leu Gly Val Gln Thr Val Asp Ile Lys Ile Pro Thr Glu Ser Met Glu 210 215 220 Val Gln Thr Glu Pro Ala Lys Pro Ala Ala Thr Ser Ile Glu Val Gln 225 230 235 240 Thr Asp Pro Trp Ile Pro Ala Pro Val Ala Thr Thr Ala Ser Thr Ala 245 250 255 Arg Arg Pro Arg Arg Lys Tyr Gly Pro Ala Ser Leu Leu Met Pro Asn 260 265 270 Tyr Ala Leu His Pro Ser Ile Ile Pro Thr Pro Gly Tyr Arg Gly Thr 275 280 285 Arg Tyr Tyr Arg Ser Arg Ser Thr Thr Ser Arg Arg Arg Lys Thr Pro 290 295 300 Ala Ser Arg Ser Arg Arg Arg Arg Arg Arg Thr Ala Ser Lys Leu Thr 305 310 315 320 Pro Ala Ala Leu Val Arg Arg Val Tyr Arg Asp Gly Arg Ala Glu Pro 325 330 335 Leu Met Leu Pro Arg Ala Arg Tyr His Pro Ser Ile Thr Thr 340 345 350 <210> 9 <211> 75 <212> PRT <213> Simian adenovirus 28 <400> 9 Met Ala Leu Thr Cys Arg Leu Arg Val Pro Ile Thr Gly Tyr Arg Gly 1 5 10 15 Arg Asn Ser Arg Arg Arg Arg Met Leu Gly Ser Gly Met Arg Arg His 20 25 30 Arg Arg Arg Arg Ala Ile Ser Lys Arg Leu Gly Gly Gly Phe Leu Thr 35 40 45 Ala Leu Ile Pro Ile Ile Ala Ala Ala Ile Gly Ala Val Pro Gly Ile 50 55 60 Ala Ser Val Ala Val Gln Ala Ser Gln Arg His 65 70 75 <210> 10 <211> 250 <212> PRT <213> Simian adenovirus 28 <400> 10 Met Glu Asp Ile Asn Phe Ser Ser Leu Ala Pro Arg His Gly Thr Arg 1 5 10 15 Pro Tyr Met Gly Thr Trp Ser Asp Ile Gly Thr Ser Gln Leu Asn Gly 20 25 30 Gly Ala Phe Asn Trp Ser Ser Ile Trp Ser Gly Leu Lys Asn Phe Gly 35 40 45 Ser Ala Ile Lys Thr Tyr Gly Asn Lys Ala Trp Asn Ser Ser Thr Gly 50 55 60 Gln Ala Leu Arg Asn Lys Leu Lys Glu Gln Asn Phe Gln Gln Lys Val 65 70 75 80 Val Asp Gly Ile Ala Ser Gly Ile Asn Gly Val Val Asp Leu Ala Asn 85 90 95 Gln Ala Val Gln Lys Gln Ile Asn Ser Arg Leu Asp Pro Pro Pro Ala 100 105 110 Ala Pro Gly Glu Met Glu Val Glu Glu Glu Leu Pro Pro Leu Glu Lys 115 120 125 Arg Gly Asp Lys Arg Pro Arg Pro Asp Met Glu Glu Thr Leu Val Thr 130 135 140 Arg Gly Asp Glu Pro Pro Pro Tyr Glu Glu Ala Ile Lys Leu Gly Met 145 150 155 160 Pro Thr Thr Arg Pro Ile Ala Pro Met Ala Thr Gly Val Met Lys Pro 165 170 175 Ser Gln Ser His Arg Pro Ala Thr Leu Asp Leu Pro Pro Ala Pro Ala 180 185 190 Ala Ala Ala Pro Ala Pro Lys Pro Val Ala Thr Pro Lys Pro Thr Ser 195 200 205 Val Gln Pro Val Ala Val Ala Arg Pro Arg Pro Gly Gly Thr Pro Arg 210 215 220 Pro Asn Ala Asn Trp Gln Ser Thr Leu Asn Ser Ile Val Gly Leu Gly 225 230 235 240 Val Gln Ser Val Lys Arg Arg Arg Cys Tyr 245 250 <210> 11 <211> 944 <212> PRT <213> Simian adenovirus 28 <400> 11 Met Ala Thr Pro Ser Met Leu Pro Gln Trp Ala Tyr Met His Ile Ala 1 5 10 15 Gly Gln Asp Ala Ser Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala 20 25 30 Arg Ala Thr Asp Thr Tyr Phe Asn Leu Gly Asn Lys Phe Arg Asn Pro 35 40 45 Thr Val Ala Pro Thr His Asp Val Thr Thr Asp Arg Ser Gln Arg Leu 50 55 60 Met Leu Arg Phe Val Pro Val Asp Arg Glu Asp Asn Thr Tyr Ser Tyr 65 70 75 80 Lys Val Arg Tyr Thr Leu Ala Val Gly Asp Asn Arg Val Leu Asp Met 85 90 95 Ala Ser Thr Phe Phe Asp Ile Arg Gly Val Leu Asp Arg Gly Pro Ser 100 105 110 Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser Leu Ala Pro Lys Gly 115 120 125 Ala Pro Asn Pro Ser Gln Trp Leu Glu Ser Thr Thr Ala Asp Glu Thr 130 135 140 Thr Thr Thr Thr Thr His Thr Phe Gly Met Ala Ser Met Lys Gly Tyr 145 150 155 160 Asp Ile Thr Lys Asp Gly Leu Gln Ile Gly Lys Glu Val Thr Ala Thr 165 170 175 Gly Asp Glu Lys Pro Ile Tyr Ala Asp Lys Lys Phe Gln Pro Glu Pro 180 185 190 Gln Val Gly Glu Glu Ser Trp Thr Asp Thr Asp Gly Thr Asn Glu Lys 195 200 205 Phe Gly Gly Arg Thr Leu Lys Ser Ala Thr Asn Met Lys Pro Cys Tyr 210 215 220 Gly Ser Phe Ala Arg Pro Thr Asn Lys Glu Gly Gly Gln Ala Lys Thr 225 230 235 240 Arg Lys Val Pro Ala Ala Glu Glu Gly Gly Ala Glu Thr Glu Glu Pro 245 250 255 Asp Ile Asp Met Val Phe Tyr Asp Asp Arg Gln Ala Ala Asp Pro Ala 260 265 270 Leu Ala Pro Glu Val Val Leu Tyr Thr Glu Asn Val Asn Leu Glu Thr 275 280 285 Pro Asp Thr His Ile Val Tyr Lys Pro Gly Thr Ser Asp Val Ser Ser 290 295 300 His Glu Asn Leu Gly Gln Gln Ala Met Pro Asn Arg Pro Asn Tyr Ile 305 310 315 320 Gly Phe Arg Asp Asn Phe Val Gly Leu Met Tyr Tyr Asn Ser Thr Gly 325 330 335 Asn Met Gly Val Leu Ala Gly Gln Ala Ser Gln Leu Asn Ala Val Val 340 345 350 Asp Leu Gln Asp Arg Asn Thr Glu Leu Ser Tyr Gln Leu Leu Leu Asp 355 360 365 Ser Leu Gly Asp Arg Thr Arg Tyr Phe Ser Met Trp Asn Gln Ala Val 370 375 380 Asp Ser Tyr Asp Pro Asp Val Arg Ile Ile Glu Asn His Gly Ile Glu 385 390 395 400 Asp Glu Leu Pro Asn Tyr Cys Phe Pro Leu Asp Gly Ile Gly Pro Gly 405 410 415 Lys Ser Tyr Gln Gly Ile Lys Glu Lys Thr Gly Glu Asp Lys Lys Trp 420 425 430 Glu Lys Asp Gly Thr Gln Ala Asn Ser Asn Glu Ile Ala Ile Gly Asn 435 440 445 Asn Leu Ala Met Glu Ile Asn Ile Gln Ala Asn Leu Trp Arg Ser Phe 450 455 460 Leu Tyr Ser Asn Val Ala Leu Tyr Leu Pro Asp Ala Tyr Lys Tyr Thr 465 470 475 480 Pro Ala Asn Ile Thr Leu Pro Ala Asn Thr Asn Thr Tyr Glu Tyr Met 485 490 495 Asn Gly Arg Val Val Ala Pro Ser Leu Val Asp Ser Tyr Ile Asn Ile 500 505 510 Gly Ala Arg Trp Ser Leu Asp Pro Met Asp Asn Val Asn Pro Phe Asn 515 520 525 His His Arg Asn Ala Gly Leu Arg Tyr Arg Ser Met Leu Leu Gly Asn 530 535 540 Gly Arg Tyr Val Pro Phe His Ile Gln Val Pro Gln Lys Phe Phe Ala 545 550 555 560 Ile Lys Asn Leu Leu Leu Leu Pro Gly Ser Tyr Thr Tyr Glu Trp Asn 565 570 575 Phe Arg Lys Asp Val Asn Met Val Leu Gln Ser Ser Leu Gly Asn Asp 580 585 590 Leu Arg Thr Asp Gly Ala Ser Ile Ser Phe Thr Ser Ile Asn Leu Tyr 595 600 605 Ala Thr Phe Phe Pro Met Ala His Asn Thr Ala Ser Thr Leu Glu Ala 610 615 620 Met Leu Arg Asn Asp Thr Asn Asp Gln Ser Phe Asn Asp Tyr Leu Ser 625 630 635 640 Ala Ala Asn Met Leu Tyr Pro Ile Pro Ala Asn Ala Thr Asn Ile Pro 645 650 655 Ile Ser Ile Pro Ser Arg Asn Trp Ala Ala Phe Arg Gly Trp Ser Phe 660 665 670 Thr Arg Leu Lys Thr Lys Glu Thr Pro Ser Leu Gly Ser Gly Phe Asp 675 680 685 Pro Tyr Phe Val Tyr Ser Gly Ser Ile Pro Tyr Leu Asp Gly Thr Phe 690 695 700 Tyr Leu Asn His Thr Phe Lys Lys Val Ser Ile Met Phe Asp Ser Ser 705 710 715 720 Val Ser Trp Pro Gly Asn Asp Arg Leu Leu Thr Pro Asn Glu Phe Glu 725 730 735 Ile Lys Arg Thr Val Asp Gly Glu Gly Tyr Asn Val Ala Gln Cys Asn 740 745 750 Met Thr Lys Asp Trp Phe Leu Val Gln Met Leu Ala Asn Tyr Asn Ile 755 760 765 Gly Tyr Gln Gly Phe Tyr Ile Pro Glu Gly Tyr Lys Asp Arg Met Tyr 770 775 780 Ser Phe Phe Arg Asn Phe Gln Pro Met Ser Arg Gln Val Val Asp Glu 785 790 795 800 Val Asn Tyr Lys Glu Tyr Gln Ala Val Thr Leu Ala Tyr Gln His Asn 805 810 815 Asn Ser Gly Phe Val Gly Tyr Leu Ala Pro Thr Met Arg Gln Gly Glu 820 825 830 Pro Tyr Pro Ala Asn Tyr Pro Tyr Pro Leu Ile Gly Thr Thr Ala Val 835 840 845 Lys Ser Val Thr Gln Lys Lys Phe Leu Cys Asp Arg Thr Met Trp Arg 850 855 860 Ile Pro Phe Ser Ser Asn Phe Met Ser Met Gly Ala Leu Thr Asp Leu 865 870 875 880 Gly Gln Asn Met Leu Tyr Ala Asn Ser Ala His Ala Leu Asp Met Thr 885 890 895 Phe Glu Val Asp Pro Met Asp Glu Pro Thr Leu Leu Tyr Val Leu Phe 900 905 910 Glu Val Phe Asp Val Val Arg Val His Gln Pro His Arg Gly Val Ile 915 920 925 Glu Ala Val Tyr Leu Arg Thr Pro Phe Ser Ala Gly Asn Ala Thr Thr 930 935 940 <210> 12 <211> 209 <212> PRT <213> Simian adenovirus 28 <400> 12 Met Ala Cys Gly Ser Gly Asn Gly Ser Ser Glu Gln Glu Leu Arg Ala 1 5 10 15 Ile Ala Arg Asp Leu Gly Cys Gly Pro Tyr Phe Leu Gly Thr Phe Asp 20 25 30 Lys Arg Phe Pro Gly Phe Met Ala Pro Asp Lys Leu Ala Cys Ala Ile 35 40 45 Val Asn Thr Ala Gly Arg Glu Thr Gly Gly Glu His Trp Leu Ala Phe 50 55 60 Gly Trp Asn Pro Arg Ser Asn Thr Cys Tyr Leu Phe Asp Pro Phe Gly 65 70 75 80 Phe Ser Asp Glu Arg Leu Lys Gln Ile Tyr Gln Phe Glu Tyr Glu Gly 85 90 95 Leu Leu Arg Arg Ser Ala Leu Ala Thr Lys Asp Arg Cys Val Thr Leu 100 105 110 Glu Lys Ser Thr Gln Thr Val Gln Gly Pro Arg Ser Ala Ala Cys Gly 115 120 125 Leu Phe Cys Cys Met Phe Leu His Ala Phe Val His Trp Pro Asp Arg 130 135 140 Pro Met Asp Gly Asn Pro Thr Met Lys Leu Leu Thr Gly Val Pro Asn 145 150 155 160 Ser Met Leu Gln Ser Pro Gln Val Gln Pro Thr Leu Arg His Asn Gln 165 170 175 Glu Ala Leu Tyr Arg Phe Leu Asn Thr His Ser Ser Tyr Phe Arg Ser 180 185 190 His Arg Ala Arg Ile Glu Lys Ala Thr Ala Phe Asp Arg Met Asp Met 195 200 205 Gln <210> 13 <211> 828 <212> PRT <213> Simian adenovirus 28 <400> 13 Met Glu Thr Gln Pro Ser Leu Pro Thr Pro Leu Gln Ala Pro Ser His 1 5 10 15 Leu Ala Pro Ser Ser Asp Glu Glu Glu Ser Leu Thr Thr Pro Pro Pro 20 25 30 Ser Pro Ala Thr Thr Thr Ser Thr Leu Glu Asp Glu Glu Glu Val Asp 35 40 45 Ala Pro Gln Glu Met Gln Asp Met Glu Asp Glu Lys Ala Glu Glu Ile 50 55 60 Glu Ala Asp Val Glu Gln Asp Pro Gly Tyr Val Thr Pro Ala Glu His 65 70 75 80 Glu Glu Glu Leu Arg Arg Phe Leu Asp Arg Glu Asp Asp Asn Arg Pro 85 90 95 Glu Gln Lys Ala Asp Gly Asp His Gln Glu Ala Gly Leu Gly Asp His 100 105 110 Val Ala Glu Tyr Leu Thr Gly Leu Gly Gly Glu Asp Val Leu Leu Lys 115 120 125 His Leu Ala Arg Gln Ser Ile Ile Val Lys Asp Ala Leu Leu Asp Arg 130 135 140 Thr Glu Val Pro Ile Ser Val Glu Glu Leu Ser Arg Ala Tyr Glu Leu 145 150 155 160 Asn Leu Phe Ser Pro Arg Leu Pro Pro Lys Arg Gln Pro Asn Gly Thr 165 170 175 Cys Glu Pro Asn Pro Arg Leu Asn Phe Tyr Pro Ala Phe Ala Val Pro 180 185 190 Glu Val Leu Ala Thr Tyr His Ile Phe Phe Lys Asn Gln Lys Ile Pro 195 200 205 Val Ser Cys Arg Ala Asn Arg Thr Arg Ala Asp Ala Leu Leu Asn Leu 210 215 220 Gly Pro Gly Ala Arg Leu Pro Asp Ile Ala Ser Leu Glu Glu Val Pro 225 230 235 240 Lys Ile Phe Glu Gly Leu Gly Ser Asp Glu Thr Arg Ala Ala Asn Ala 245 250 255 Leu Gln Gln Gly Glu Asn Gly Met Asp Glu His His Ser Ala Leu Val 260 265 270 Glu Leu Glu Gly Asp Asn Ala Arg Leu Ala Val Leu Lys Arg Ser Ile 275 280 285 Glu Val Thr His Phe Ala Tyr Pro Ala Val Asn Leu Pro Pro Lys Val 290 295 300 Met Ser Ala Val Met Asp Gln Leu Leu Ile Lys Arg Ala Ser Pro Leu 305 310 315 320 Pro Glu Asp Gln Asn Met Gln Asp Pro Asp Ala Ser Asp Glu Gly Lys 325 330 335 Pro Val Val Ser Asp Glu Gln Leu Ser Arg Trp Leu Gly Thr Asn Ser 340 345 350 Pro Arg Asp Leu Glu Glu Arg Arg Lys Leu Met Met Ala Val Val Leu 355 360 365 Val Thr Val Glu Leu Glu Cys Leu Arg Arg Phe Phe Thr Asp Pro Glu 370 375 380 Thr Leu Arg Lys Leu Glu Glu Asn Leu His Tyr Thr Phe Arg His Gly 385 390 395 400 Phe Val Arg Gln Ala Cys Lys Ile Ser Asn Val Glu Leu Thr Asn Leu 405 410 415 Val Ser Tyr Met Gly Ile Leu His Glu Asn Arg Leu Gly Gln Ser Val 420 425 430 Leu His Thr Thr Leu Lys Gly Glu Ala Arg Arg Asp Tyr Ile Arg Asp 435 440 445 Cys Val Tyr Leu Tyr Leu Cys His Thr Trp Gln Thr Gly Met Gly Val 450 455 460 Trp Gln Gln Cys Leu Glu Glu Gln Asn Leu Lys Glu Leu Asp Lys Leu 465 470 475 480 Leu Gln Arg Ser Leu Lys Ala Leu Trp Thr Gly Phe Asp Glu Arg Thr 485 490 495 Val Ala Ser Asp Leu Ala Asp Ile Ile Phe Pro Glu Arg Leu Arg Val 500 505 510 Thr Leu Arg Asn Gly Leu Pro Asp Phe Met Ser Gln Ser Met Leu Asn 515 520 525 Asn Phe Arg Ser Phe Ile Leu Glu Arg Ser Gly Ile Leu Pro Ala Thr 530 535 540 Cys Cys Ala Leu Pro Ser Asp Phe Val Pro Leu Thr Tyr Arg Glu Cys 545 550 555 560 Pro Pro Pro Leu Trp Ser His Cys Tyr Leu Phe Arg Leu Ala Asn Tyr 565 570 575 Leu Ser Tyr His Ser Asp Val Ile Glu Asp Val Ser Gly Asp Gly Leu 580 585 590 Leu Glu Cys His Cys Arg Cys Asn Leu Cys Thr Pro His Arg Ser Leu 595 600 605 Ala Cys Asn Pro Gln Leu Leu Ser Glu Thr Gln Ile Ile Gly Thr Phe 610 615 620 Glu Leu Gln Gly Pro Ser Ser Glu Gly Glu Gly Ser Ser Pro Gly Gln 625 630 635 640 Ser Leu Lys Leu Thr Pro Gly Leu Trp Thr Ser Ala Tyr Leu Arg Lys 645 650 655 Phe Ala Pro Glu Asp Tyr His Pro Tyr Glu Ile Arg Phe Tyr Glu Asp 660 665 670 Gln Ser Gln Pro Pro Lys Thr Glu Leu Ser Ala Cys Val Ile Thr Gln 675 680 685 Gly Ala Ile Leu Ala Gln Leu Gln Ala Ile Gln Lys Ser Arg Gln Glu 690 695 700 Phe Leu Leu Lys Lys Gly Asn Gly Val Tyr Leu Asp Pro Gln Thr Gly 705 710 715 720 Glu Glu Leu Asn Thr Arg Phe Pro Gln Asp Val Pro Ala Pro Arg Lys 725 730 735 Gln Glu Val Glu Gly Ala Ala Ala Ala Pro Arg Gly Tyr Gly Gly Arg 740 745 750 Leu Gly Gln Ser Gly Arg Gly Gly Asp Gly Arg Leu Gly Gln Pro Gly 755 760 765 Arg Gly Gly Gly Gln Pro Gly Gly Arg Gln Phe Gly Gly Gly Arg Arg 770 775 780 Gly Gly Arg Gly Gly Gly Arg Ser Asn Arg Arg Gln Thr Val Val Leu 785 790 795 800 Gly Ser Gly Asp Lys Gln Gly Pro Arg Gln Gln Gln His Gly Tyr Asn 805 810 815 Leu Arg Ser Gly Gly Gly Pro Ala Ala Ser Gln Gln 820 825 <210> 14 <211> 227 <212> PRT <213> Simian adenovirus 28 <400> 14 Met Ser Lys Glu Ile Pro Thr Pro Tyr Met Trp Ser Tyr Gln Pro Gln 1 5 10 15 Met Gly Leu Ala Ala Gly Ala Ser Gln Asp Tyr Ser Thr Arg Met Asn 20 25 30 Trp Leu Ser Ala Gly Pro Ser Met Ile Ser Arg Val Asn Asp Ile Arg 35 40 45 Ala Tyr Arg Asn Gln Leu Leu Leu Glu Gln Ser Ala Leu Thr Thr Thr 50 55 60 Pro Arg Gln His Leu Asn Pro Arg Asn Trp Pro Ala Ala Leu Val Tyr 65 70 75 80 Gln Glu Thr Pro Ala Pro Thr Thr Val Leu Leu Pro Arg Asp Ala Gln 85 90 95 Ala Glu Val Gln Met Thr Asn Ala Gly Val Gln Leu Ala Gly Gly Ser 100 105 110 Ala Leu Cys Arg His Arg Pro Gln Gln Ser Ile Lys Arg Leu Val Ile 115 120 125 Arg Gly Arg Gly Ile Gln Leu Asn Asp Glu Ser Val Ser Ser Ser Leu 130 135 140 Gly Leu Arg Pro Asp Gly Val Phe Gln Ile Ala Gly Cys Gly Arg Ser 145 150 155 160 Ser Phe Thr Pro Arg Gln Ala Val Leu Thr Leu Glu Ser Ser Ser Ser 165 170 175 Gln Pro Arg Ser Gly Gly Ile Gly Thr Leu Gln Phe Val Glu Glu Phe 180 185 190 Thr Pro Ser Val Tyr Phe Asn Pro Phe Ser Gly Ser Pro Gly Gln Tyr 195 200 205 Pro Asp Glu Phe Ile Pro Asn Phe Asp Ala Ile Ser Glu Ser Val Asp 210 215 220 Gly Tyr Asp 225 <210> 15 <211> 105 <212> PRT <213> Simian adenovirus 28 <400> 15 Met Ser Gly Gly Ala Ala Glu Leu Ala Arg Leu Arg His Leu Asp His 1 5 10 15 Cys Arg Arg Phe Arg Cys Phe Ala Arg Glu Leu Thr Glu Phe Ile Tyr 20 25 30 Phe Glu Leu Pro Glu Glu His Pro Gln Gly Pro Ala His Gly Val Arg 35 40 45 Ile Thr Ile Glu Gly Gly Ile Asp Ser Arg Leu His Arg Ile Phe Cys 50 55 60 Gln Arg Pro Val Leu Ile Glu Arg Asp Gln Gly Thr Thr Thr Val Ser 65 70 75 80 Ile Tyr Cys Ile Cys Asn His Pro Gly Leu His Glu Ser Leu Cys Cys 85 90 95 Leu Ile Cys Ala Glu Phe Asn Lys Asn 100 105 <210> 16 <211> 178 <212> PRT <213> Simian adenovirus 28 <400> 16 Met Gly Val Val Leu Val Ala Leu Ala Leu Leu Ser Leu Leu Gly Leu 1 5 10 15 Gly Ser Thr Thr Leu Arg Asn Gln Pro Leu Leu Leu Asp Pro Asn Asp 20 25 30 Val Asp Pro Cys Leu Asp Phe Asp Pro Glu Asn Cys Thr Leu Thr Phe 35 40 45 Ala Pro Glu Thr Ser Arg Phe Cys Gly Val Val Ile Arg Cys Gly Phe 50 55 60 Glu Cys Arg Pro Ile Glu Ile Thr His Asn Asn Lys Thr Trp Asn Asn 65 70 75 80 Thr Leu Phe Thr Thr Trp Ser Pro Gly Asp Pro Gln Trp Tyr Thr Val 85 90 95 Ser Val Arg Gly Pro Asp Gly Ser Val Arg Met Ala Asn Asn Thr Phe 100 105 110 Ile Phe Ala Glu Met Cys Asp Met Ala Met Phe Met Ser Arg Gln Tyr 115 120 125 Asp Leu Trp Pro Pro Ser Lys Glu Asn Ile Val Ala Phe Ser Ile Ala 130 135 140 Tyr Cys Leu Gly Thr Cys Ile Ile Thr Ala Ile Met Cys Val Ser Ile 145 150 155 160 His Leu Leu Ile Ala Ile Arg Pro Lys Asn Asn Gln Glu Lys Glu Lys 165 170 175 Met Pro <210> 17 <211> 188 <212> PRT <213> Simian adenovirus 28 <400> 17 Met Ile Phe Phe Ala Thr Leu Ile Thr Ile Gly Ile Val Gln Gly Gln 1 5 10 15 Asp Ile Thr Ile Gly Tyr Val Gly Asn Asn Ile Thr Leu Leu Gly Pro 20 25 30 Pro Thr Gly Thr Ile Pro Thr Trp Tyr Lys Ile Tyr Glu Arg Gly Trp 35 40 45 Trp Ile Arg Pro Cys Asp Gln Gly Gly Ser Lys Tyr Ile Cys Gly Arg 50 55 60 Asp Ile Thr Ile Thr Asn Leu Asn Lys Asn Asp Asn Gly Tyr Tyr Phe 65 70 75 80 Cys Asn Asn Tyr Gly Gly Gly Lys Lys Ser Tyr Thr Leu Glu Val Arg 85 90 95 Asp Pro Thr Thr Leu Ala Pro His Thr Thr Phe Ser Ser Ser Thr Ser 100 105 110 Arg Asn Thr His Glu Ala Ala Tyr Ala Arg Ala Met Leu Gln Lys Ile 115 120 125 Asn Glu Thr Ile Asn Ser Thr Ile Ser His Asn Pro Asp Glu Ile Pro 130 135 140 Lys Ser Met Ile Gly Ile Ile Val Ala Val Ala Val Gly Met Ala Ile 145 150 155 160 Ile Ile Ile Cys Met Ile Val Tyr Ala Cys Cys Tyr Arg Lys Phe Gln 165 170 175 Asp Glu Lys Gly Asp Pro Leu Leu Ser Phe Asp Ile 180 185 <210> 18 <211> 106 <212> PRT <213> Simian adenovirus 28 <400> 18 Met Lys Gly Val Gly Ile Leu Val Leu Ser Thr Leu Ile Tyr Ser Val 1 5 10 15 Ile Pro Ile Ser Ile Asn Val Gln Thr Thr Leu Asn Glu Thr Gly Asn 20 25 30 His Ser Thr Thr Ser His Thr Pro Pro Pro Leu Ser Thr His Pro Gln 35 40 45 Ser Lys Asp Ala Ile Gln Leu Gln Leu Thr Ile Leu Ile Val Ile Gly 50 55 60 Leu Thr Ile Leu Ala Val Ile Leu Tyr Phe Ile Phe Cys Arg Gln Ile 65 70 75 80 Pro Asn Val Val Lys Pro Thr Arg Arg Pro Ile Tyr Arg Ser Ile Ile 85 90 95 Ser Lys Pro His Met Ala Leu Asn Glu Ile 100 105 <210> 19 <211> 91 <212> PRT <213> Simian adenovirus 28 <400> 19 Met Ile Pro Arg Asn Phe Phe Phe Thr Ile Leu Ile Cys Ala Phe Asn 1 5 10 15 Val Cys Ala Thr Phe Ala Thr Val Ala Asn Val Thr Pro Asp Cys Ile 20 25 30 Gly Ala Phe Ala Ser Tyr Val Leu Phe Ala Phe Ile Thr Cys Ile Cys 35 40 45 Val Cys Ser Ile Val Cys Leu Val Ile Asn Phe Phe Gln Leu Val Asp 50 55 60 Trp Val Phe Val Arg Ile Ala Tyr Leu Arg His His Pro Glu Tyr Arg 65 70 75 80 Asn Gln Asn Val Ala Ala Ile Leu Arg Leu Ile 85 90 <210> 20 <211> 132 <212> PRT <213> Simian adenovirus 28 <400> 20 Met Thr Asp Pro His Ala Ala Ala Glu Glu Leu Leu Asp Met Asp Gly 1 5 10 15 Arg Ala Ser Glu Gln Arg Leu Ala Gln Leu Arg Ile Arg Gln Gln Gln 20 25 30 Glu Arg Ala Ala Lys Glu Leu Arg Asp Ala Ile Glu Ile His Gln Cys 35 40 45 Lys Lys Gly Ile Phe Cys Leu Val Lys Gln Ala Lys Ile Ser Tyr Glu 50 55 60 Ile Thr Ala Asn Asp His Arg Leu Ser Tyr Glu Leu Gly Pro Gln Arg 65 70 75 80 Gln Lys Phe Thr Cys Met Val Gly Ile Asn Pro Ile Val Ile Thr Gln 85 90 95 Gln Ala Gly Asp Thr Lys Gly Cys Ile His Cys Ser Cys Glu Ser Thr 100 105 110 Glu Cys Ile Tyr Thr Leu Leu Lys Thr Leu Cys Gly Leu Arg Asp Leu 115 120 125 Leu Pro Met Asn 130 <210> 21 <211> 323 <212> PRT <213> Simian adenovirus 28 <400> 21 Met Ala Lys Arg Ala Arg Leu Ser Ser Ser Phe Asn Pro Val Tyr Pro 1 5 10 15 Tyr Glu Asp Glu Ser Ser Ser Gln His Pro Phe Ile Asn Pro Gly Phe 20 25 30 Ile Ser Pro Asn Gly Phe Thr Gln Ser Pro Asp Gly Ala Leu Thr Leu 35 40 45 Lys Cys Val Ala Pro Leu Thr Thr Thr Ser Gly Ser Leu Asp Ile Lys 50 55 60 Val Gly Gly Gly Leu Lys Val Asp Ser Thr Asp Gly Ser Leu Glu Glu 65 70 75 80 Asn Ile Ser Thr Thr Ala Pro Leu Asn Lys Ser Asn His Ser Ile Gly 85 90 95 Leu Ala Val Gly Asn Gly Leu Gln Thr Asn Glu Ser Lys Leu Cys Ala 100 105 110 Lys Leu Gly Glu Glu Leu Thr Phe Asp Ser Ser Asn Ala Ile Thr Ile 115 120 125 Lys Asn Asn Thr Leu Trp Thr Gly Ala Lys Pro Ser Thr Asn Cys Lys 130 135 140 Ile Gln Glu Asp Ala Asp Ala Leu Asp Cys Lys Leu Thr Leu Val Leu 145 150 155 160 Val Lys Asn Gly Gly Leu Val Asn Ala Tyr Val Ser Leu Ile Gly Asp 165 170 175 Ser Asp Tyr Val Asn Thr Leu Phe Thr Lys Lys Thr Ala Ser Ile Ser 180 185 190 Val Glu Leu Ala Phe Asp Ser Ser Gly Gln Ile Leu Thr Ser Leu Ser 195 200 205 Ser Leu Lys Thr Ser Leu Asn Phe Lys His Asn Gln Asp Met Ala Thr 210 215 220 Glu Thr Ile Ser Ala Lys Gly Phe Met Pro Ser Thr Thr Ala Tyr Pro 225 230 235 240 Phe Asn Thr Gln Ala Thr Ser Ser Arg Asp Asn Glu Asp Tyr Ile Phe 245 250 255 Gly Lys Cys Tyr Tyr Arg Ala Ser Tyr Gly Ala Leu Tyr Thr Leu Asp 260 265 270 Val Thr Val Ile Leu Asn Arg Arg Met Thr Ala Ala Gly Met Ala Tyr 275 280 285 Ala Met Asn Phe Thr Trp Leu Leu Asp Ala Thr Asp Ala Pro Glu Asn 290 295 300 Thr Thr Thr Thr Leu Val Thr Ser Pro Phe Ser Phe Ser Tyr Ile Arg 305 310 315 320 Glu Asp Asp <210> 22 <211> 550 <212> DNA <213> Simian adenovirus 28 <220> <221> CDS <222> (2)..(544) <223> label=Elb\19K <400> 22 c atg gag gtt tgg gct atc ttg gaa gat ctc agg cag act agg caa ctg 49 Met Glu Val Trp Ala Ile Leu Glu Asp Leu Arg Gln Thr Arg Gln Leu 1 5 10 15 cta gaa aac gcc tcg gac gga gtc tct agt ctt tgg aga ttc tgg ttc 97 Leu Glu Asn Ala Ser Asp Gly Val Ser Ser Leu Trp Arg Phe Trp Phe 20 25 30 ggt ggt gat cta gct agg cta gtc ttt agg gta aaa cgg gag tat agt 145 Gly Gly Asp Leu Ala Arg Leu Val Phe Arg Val Lys Arg Glu Tyr Ser 35 40 45 gaa gaa ttt gaa aag tta ttg gaa gac agt cca gga ctt ttt gaa gcc 193 Glu Glu Phe Glu Lys Leu Leu Glu Asp Ser Pro Gly Leu Phe Glu Ala 50 55 60 ctt aac ttg ggc cac cag gct cat ttt aag gag aag gtt tta tca gtt 241 Leu Asn Leu Gly His Gln Ala His Phe Lys Glu Lys Val Leu Ser Val 65 70 75 80 tta gat ttt tct acc cct ggt aga act gct gct gct gta gct ttc ctt 289 Leu Asp Phe Ser Thr Pro Gly Arg Thr Ala Ala Ala Val Ala Phe Leu 85 90 95 act ttt ata ttg gat aaa tgg atc cca caa acc cac ttc agc aag gga 337 Thr Phe Ile Leu Asp Lys Trp Ile Pro Gln Thr His Phe Ser Lys Gly 100 105 110 tac gtc ttg gat ttc ata gca gca gct ttg tgg aga aca tgg aag gcc 385 Tyr Val Leu Asp Phe Ile Ala Ala Ala Leu Trp Arg Thr Trp Lys Ala 115 120 125 cgc agg ctg agg ata atc tta gat tac tgg cca gtg cag cct ctg ggc 433 Arg Arg Leu Arg Ile Ile Leu Asp Tyr Trp Pro Val Gln Pro Leu Gly 130 135 140 gta gcg gcg atc ctg aga cac cca ccg gcc atg cca gcg gtt ttg gag 481 Val Ala Ala Ile Leu Arg His Pro Pro Ala Met Pro Ala Val Leu Glu 145 150 155 160 gag gag cag cag gag gac aac ccg aga gcc ggc ctg gac cct ccg gtg 529 Glu Glu Gln Gln Glu Asp Asn Pro Arg Ala Gly Leu Asp Pro Pro Val 165 170 175 gag gag gcg gag gag tagctg 550 Glu Glu Ala Glu Glu 180 <210> 23 <211> 181 <212> PRT <213> Simian adenovirus 28 <400> 23 Met Glu Val Trp Ala Ile Leu Glu Asp Leu Arg Gln Thr Arg Gln Leu 1 5 10 15 Leu Glu Asn Ala Ser Asp Gly Val Ser Ser Leu Trp Arg Phe Trp Phe 20 25 30 Gly Gly Asp Leu Ala Arg Leu Val Phe Arg Val Lys Arg Glu Tyr Ser 35 40 45 Glu Glu Phe Glu Lys Leu Leu Glu Asp Ser Pro Gly Leu Phe Glu Ala 50 55 60 Leu Asn Leu Gly His Gln Ala His Phe Lys Glu Lys Val Leu Ser Val 65 70 75 80 Leu Asp Phe Ser Thr Pro Gly Arg Thr Ala Ala Ala Val Ala Phe Leu 85 90 95 Thr Phe Ile Leu Asp Lys Trp Ile Pro Gln Thr His Phe Ser Lys Gly 100 105 110 Tyr Val Leu Asp Phe Ile Ala Ala Ala Leu Trp Arg Thr Trp Lys Ala 115 120 125 Arg Arg Leu Arg Ile Ile Leu Asp Tyr Trp Pro Val Gln Pro Leu Gly 130 135 140 Val Ala Ala Ile Leu Arg His Pro Pro Ala Met Pro Ala Val Leu Glu 145 150 155 160 Glu Glu Gln Gln Glu Asp Asn Pro Arg Ala Gly Leu Asp Pro Pro Val 165 170 175 Glu Glu Ala Glu Glu 180 <210> 24 <211> 5100 <212> DNA <213> Simian adenovirus 28 <220> <221> CDS <222> (7)..(621) <223> label=22K <220> <221> CDS <222> (1916)..(2359) <223> label=E3\CR1-alpha <220> <221> CDS <222> (2736)..(3473) <223> label=E3\CR1-beta <220> <221> CDS <222> (4674)..(5090) <223> label=E3\RID-beta <400> 24 ctcagg atg tcc cag cgc cga gga agc aag aag ttg aag gtg cag ctg 48 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln Leu 1 5 10 ccg ccc cca gag gat atg gag gaa gac tgg gac agt cag gca gag gag 96 Pro Pro Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu Glu 15 20 25 30 gag atg gaa gat tgg gac agc cag gca gag gag gcg gac agc ctg gag 144 Glu Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Ala Asp Ser Leu Glu 35 40 45 gaa gac agt ttg gag gag gaa gac gag gag gca gag gag gtg gaa gaa 192 Glu Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu 50 55 60 gca acc gcc gcc aaa cag ttg tcc tcg gca gcg gag aca agc aag gtc 240 Ala Thr Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Val 65 70 75 cca gac agc agc agc acg gct aca atc tcc gct ccg ggg ggg gcc cag 288 Pro Asp Ser Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Gly Ala Gln 80 85 90 cgg cgt ccc aac agt aga tgg gac gag acc ggg cga ttc ccg aac ccg 336 Arg Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe Pro Asn Pro 95 100 105 110 acc acc gct tcc aag acc ggt aag aag gag cgg cag gga tac aag tcc 384 Thr Thr Ala Ser Lys Thr Gly Lys Lys Glu Arg Gln Gly Tyr Lys Ser 115 120 125 tgg cgg ggg cat aag aat gcc atc atc tcc tgc ttg cat gaa tgc ggg 432 Trp Arg Gly His Lys Asn Ala Ile Ile Ser Cys Leu His Glu Cys Gly 130 135 140 ggc aac ata tcc ttc acc cgg cgc tac ctg ctc ttc cac cac ggg gtg 480 Gly Asn Ile Ser Phe Thr Arg Arg Tyr Leu Leu Phe His His Gly Val 145 150 155 aac ttc ccc cgc aat gtc ttg cat tac tac cgt cac ctc cac agc ccc 528 Asn Phe Pro Arg Asn Val Leu His Tyr Tyr Arg His Leu His Ser Pro 160 165 170 tac tac aac cag caa gtc ccg gca gcc tcg gca gag aaa gac agc agc 576 Tyr Tyr Asn Gln Gln Val Pro Ala Ala Ser Ala Glu Lys Asp Ser Ser 175 180 185 190 agc agc agc agc ggg gac ctc cag cag aaa acc agc agc agc agt 621 Ser Ser Ser Ser Gly Asp Leu Gln Gln Lys Thr Ser Ser Ser Ser 195 200 205 tagaaaatcc agtgcagcag gaggaggact gaggatcaca gcgaacgagc cagcgcagac 681 ccgagagctg agaaacagga tctttccaac cctctatgcc atcttccagc agagtcgggg 741 gcaagagcag gaactgaaag taaaaaaccg atctctgcgc tcgctcaccc gaagttgttt 801 gtatcacaag agcgaagacc aacttcagcg cactctcgag gacgccgagg ctctcttcaa 861 caagtactgc gcgctgactc ttaaagagta gcccgcgccc gcgctcgctc gaaaaaggcg 921 ggaattacgt cacccttggc acctgtcctt tgccctcgtc atgagtaaag aaattcccac 981 gccttacatg tggagctatc agccccaaat gggactggca gcaggcgcct cccaggacta 1041 ctccacccgc atgaattggc tcagcgccgg cccctcgatg atctcacggg ttaatgatat 1101 acgagcttac cgaaaccagt tactcctaga acagtcagca ctcaccacca caccccgcca 1161 acaccttaat ccccggaatt ggcccgccgc cctggtgtac caggaaactc ccgctcccac 1221 caccgtacta cttcctcgag acgcccaggc cgaagttcag atgactaacg caggtgtaca 1281 gctggcgggc ggttccgccc tgtgtcgtca ccggcctcag cagagtataa aacgcctggt 1341 gatcagaggc cgaggtatcc agcttaacga cgagtcggtg agctcttcgc ttggtctgcg 1401 accagacgga gtcttccaaa ttgccggctg tgggagatct tccttcactc ctcgtcaggc 1461 tgtcctgact ttggagagtt cgtcctcgca accccgctcg ggcggcatcg ggactctcca 1521 gtttgtggag gagtttactc cctctgtcta cttcaacccc ttctccggct ctcctggcca 1581 gtacccggac gagttcatac cgaacttcga cgcaatcagc gagtcagtgg atggctatga 1641 ttgatgtctg gtggcgcggc tgagttagct cgactgcgac atctagacca ctgccgccgc 1701 tttcgctgtt tcgcccggga actcaccgag ttcatctact tcgaactccc cgaggagcac 1761 cctcagggac cggcccacgg agtgcggatt accatcgaag ggggaataga ctctcgcctg 1821 catcggatct tctgtcagcg gccagtgctg atcgaacgcg accagggaac tacaacagtc 1881 tccatctact gcatctgtaa ccaccccgga ttgc atg aaa gcc ttt gct gtc tta 1936 Met Lys Ala Phe Ala Val Leu 210 ttt gtg ctg agt tta ata aaa act gag ttc aga ccc tcc tac gga cta 1984 Phe Val Leu Ser Leu Ile Lys Thr Glu Phe Arg Pro Ser Tyr Gly Leu 215 220 225 ccg ctt ctt caa ccc gga ctt tac aac acc agc cag acc ctc cgt tcc 2032 Pro Leu Leu Gln Pro Gly Leu Tyr Asn Thr Ser Gln Thr Leu Arg Ser 230 235 240 agc cag aag acc cag gcc ctt cct ctg atc cag gac tct aat tct acc 2080 Ser Gln Lys Thr Gln Ala Leu Pro Leu Ile Gln Asp Ser Asn Ser Thr 245 250 255 260 tcc cca gca cca tcc cct act aac ctt ccc gaa act aac aac ctc gga 2128 Ser Pro Ala Pro Ser Pro Thr Asn Leu Pro Glu Thr Asn Asn Leu Gly 265 270 275 gct cag ctg caa cac cgc ttc tcc aga agc ctc ctt tct gcc aat act 2176 Ala Gln Leu Gln His Arg Phe Ser Arg Ser Leu Leu Ser Ala Asn Thr 280 285 290 act act ccc aaa acc gga ggt gag ctc cgc ggt ctc cct act gac aac 2224 Thr Thr Pro Lys Thr Gly Gly Glu Leu Arg Gly Leu Pro Thr Asp Asn 295 300 305 ccc tgg gcg gta gca ggt ttt gta gcg tta gga gta gtt gcg ggt ggg 2272 Pro Trp Ala Val Ala Gly Phe Val Ala Leu Gly Val Val Ala Gly Gly 310 315 320 ctg gtg ctt atc ctc tgc tac cta tac aca cct tgc tgt gct tat tta 2320 Leu Val Leu Ile Leu Cys Tyr Leu Tyr Thr Pro Cys Cys Ala Tyr Leu 325 330 335 340 gta ata ttg tgc tgc tgg ttt aag aaa tgg ggg tcg tac tagtagcgct 2369 Val Ile Leu Cys Cys Trp Phe Lys Lys Trp Gly Ser Tyr 345 350 tgctttactt tcgcttttgg gtctgggctc tactacgcta agaaatcagc ctttgctatt 2429 agatcccaat gatgttgatc catgtctgga ctttgatcca gagaactgca cactcacttt 2489 tgcacctgaa acaagtcgct tctgtggagt tgttattagg tgcggatttg aatgcaggcc 2549 cattgagatt acacacaata acaaaacttg gaacaatacc ttatttacca catggtctcc 2609 aggagatcct cagtggtata ctgtctctgt ccggggtcct gacggttccg tccgcatggc 2669 taataacact ttcatttttg ctgaaatgtg cgatatggcc atgttcatga gcagacagta 2729 tgacct atg gcc tcc cag caa aga gaa cat tgt ggc att ctc cat tgc 2777 Met Ala Ser Gln Gln Arg Glu His Cys Gly Ile Leu His Cys 355 360 365 tta ttg ctt ggg tac atg cat cat cac tgc tat cat gtg tgt gag cat 2825 Leu Leu Leu Gly Tyr Met His His His Cys Tyr His Val Cys Glu His 370 375 380 aca ctt gct tat agc cat tcg ccc aaa aaa caa tca aga aaa aga gaa 2873 Thr Leu Ala Tyr Ser His Ser Pro Lys Lys Gln Ser Arg Lys Arg Glu 385 390 395 aat gcc ctg att ata aat ttc tat tta cag aaa atg acc tct gtt tca 2921 Asn Ala Leu Ile Ile Asn Phe Tyr Leu Gln Lys Met Thr Ser Val Ser 400 405 410 415 gct ctc ata ttt gct act att atg gct gtt caa gga cag gct gtt caa 2969 Ala Leu Ile Phe Ala Thr Ile Met Ala Val Gln Gly Gln Ala Val Gln 420 425 430 gga cag aca ctt att aat gtt cat cct gga act aat cat acc ttg gtg 3017 Gly Gln Thr Leu Ile Asn Val His Pro Gly Thr Asn His Thr Leu Val 435 440 445 gtt cct aat aac tat tca aat att gaa tgg caa tgg ttc aca aac aac 3065 Val Pro Asn Asn Tyr Ser Asn Ile Glu Trp Gln Trp Phe Thr Asn Asn 450 455 460 gta tgg tat gaa cca tgc gaa cat tac agc cta ttc att tgc aat cat 3113 Val Trp Tyr Glu Pro Cys Glu His Tyr Ser Leu Phe Ile Cys Asn His 465 470 475 aat tta act tta atc aat gtc agc aca ata cac aaa gga tac tat tat 3161 Asn Leu Thr Leu Ile Asn Val Ser Thr Ile His Lys Gly Tyr Tyr Tyr 480 485 490 495 aga tat gac aac cac agc att gat cct aca ata tat cta gta cgt gta 3209 Arg Tyr Asp Asn His Ser Ile Asp Pro Thr Ile Tyr Leu Val Arg Val 500 505 510 aat cca att aac aaa cct ata ccc aaa gct ttc tct aga act aca ata 3257 Asn Pro Ile Asn Lys Pro Ile Pro Lys Ala Phe Ser Arg Thr Thr Ile 515 520 525 caa aac ttt aaa aca gca att tta ctt aat ttt aaa acc aaa aat att 3305 Gln Asn Phe Lys Thr Ala Ile Leu Leu Asn Phe Lys Thr Lys Asn Ile 530 535 540 aca ggc aat ata ctt ccc act act ccc act gaa aaa aat aca cct aat 3353 Thr Gly Asn Ile Leu Pro Thr Thr Pro Thr Glu Lys Asn Thr Pro Asn 545 550 555 tca ata ttt gaa atc atc att gca ctg tta gca gta ggc ata aca atc 3401 Ser Ile Phe Glu Ile Ile Ile Ala Leu Leu Ala Val Gly Ile Thr Ile 560 565 570 575 ata cta tgt atg ata att tat gct cac tgt tat aaa aaa att cac cac 3449 Ile Leu Cys Met Ile Ile Tyr Ala His Cys Tyr Lys Lys Ile His His 580 585 590 aaa aaa gaa cca cta cta agc ttt taatttcttt tttatacagc catgattttc 3503 Lys Lys Glu Pro Leu Leu Ser Phe 595 ttcgcaactc ttattactat tggcattgtt caagggcaag atatcacaat tggatatgta 3563 ggcaataata ttaccctatt aggtccccca acaggaacaa tccctacctg gtacaaaata 3623 tatgaaagag ggtggtggat tagaccctgc gaccaaggag gtagtaaata catttgtggt 3683 agagacataa ccatcaccaa tcttaataaa aacgataatg gctactattt ttgcaataac 3743 tatggaggtg gtaaaaagtc ttacacactt gaagtaagag accccaccac tttagcacca 3803 cataccactt tctccagcag cacgtctaga aacacacatg aggcagctta tgccagagca 3863 atgcttcaaa aaattaatga aacaataaat tctacaatct ctcataatcc agacgaaatt 3923 cccaaatcaa tgattggcat tattgtagcc gtggcagttg gaatggcaat cataataatt 3983 tgtatgatcg tctatgcttg ctgctataga aagtttcaag atgaaaaagg agacccacta 4043 ctaagctttg atatttaatt tctttataga aacatgaaag gagtaggtat cctagttctt 4103 tcaactttaa tctactcagt gatccctatc agcatcaatg tgcagactac tttaaatgaa 4163 actggaaacc actcaactac ctcacataca cctcccccgc tttctaccca ccctcaatcc 4223 aaagatgcca tacaactaca actcaccatc cttattgtga ttgggttaac tatccttgct 4283 gttatccttt actttatctt ttgccgccaa atacccaatg tagttaaacc taccagacgt 4343 cccatctatc gatcaataat cagcaaaccc cacatggctc taaatgaaat ttaatctttc 4403 tcttcacagt atggtgatca actatgatcc ctagaaattt cttcttcacc atacttatct 4463 gcgctttcaa tgtctgtgct acattcgcca cagtcgccaa tgtgacacca gattgtatag 4523 gggcatttgc ttcctacgta ctatttgcct tcattacctg catctgcgtt tgtagcatag 4583 tctgcctggt tatcaacttc tttcaactag tagactgggt ttttgtacgc attgcctacc 4643 tacgacatca ccctgaatac cgcaaccaaa atg ttg cag caa ttc tta ggc tca 4697 Met Leu Gln Gln Phe Leu Gly Ser 600 605 ttt aaa acc atg caa act ctg cta ctg ctt ctg cta gtt ata cac caa 4745 Phe Lys Thr Met Gln Thr Leu Leu Leu Leu Leu Leu Val Ile His Gln 610 615 620 tgt gcc tca aac ccc aca agc ccc aca aaa tta gat cta aga aaa tgt 4793 Cys Ala Ser Asn Pro Thr Ser Pro Thr Lys Leu Asp Leu Arg Lys Cys 625 630 635 aaa ttt caa gaa cca tgg aaa ttc ctt gat tgc tat cat gaa aca tct 4841 Lys Phe Gln Glu Pro Trp Lys Phe Leu Asp Cys Tyr His Glu Thr Ser 640 645 650 655 gat ttc ccc aca tac tgg att aca atc att ggg gtt gtt aat cta gtc 4889 Asp Phe Pro Thr Tyr Trp Ile Thr Ile Ile Gly Val Val Asn Leu Val 660 665 670 tct tgc aca cta ttc tct ttc ctt gtt tac cac tta ttt gat ttt gga 4937 Ser Cys Thr Leu Phe Ser Phe Leu Val Tyr His Leu Phe Asp Phe Gly 675 680 685 tgg aac gcc ctt aat gca ctc act tac cca caa gaa cca gag gaa cat 4985 Trp Asn Ala Leu Asn Ala Leu Thr Tyr Pro Gln Glu Pro Glu Glu His 690 695 700 ata cca cta cag aac ata caa cca tta gca cta gaa tat gaa aat gag 5033 Ile Pro Leu Gln Asn Ile Gln Pro Leu Ala Leu Glu Tyr Glu Asn Glu 705 710 715 cca cag cct cca cta ctc cct gcc att agc tac ttc aac cta acc ggt 5081 Pro Gln Pro Pro Leu Leu Pro Ala Ile Ser Tyr Phe Asn Leu Thr Gly 720 725 730 735 gga gat gac tgacccacac 5100 Gly Asp Asp <210> 25 <211> 205 <212> PRT <213> Simian adenovirus 28 <400> 25 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu Glu Glu Met 20 25 30 Glu Asp Trp Asp Ser Gln Ala Glu Glu Ala Asp Ser Leu Glu Glu Asp 35 40 45 Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu Ala Thr 50 55 60 Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Val Pro Asp 65 70 75 80 Ser Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Gly Ala Gln Arg Arg 85 90 95 Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe Pro Asn Pro Thr Thr 100 105 110 Ala Ser Lys Thr Gly Lys Lys Glu Arg Gln Gly Tyr Lys Ser Trp Arg 115 120 125 Gly His Lys Asn Ala Ile Ile Ser Cys Leu His Glu Cys Gly Gly Asn 130 135 140 Ile Ser Phe Thr Arg Arg Tyr Leu Leu Phe His His Gly Val Asn Phe 145 150 155 160 Pro Arg Asn Val Leu His Tyr Tyr Arg His Leu His Ser Pro Tyr Tyr 165 170 175 Asn Gln Gln Val Pro Ala Ala Ser Ala Glu Lys Asp Ser Ser Ser Ser 180 185 190 Ser Ser Gly Asp Leu Gln Gln Lys Thr Ser Ser Ser Ser 195 200 205 <210> 26 <211> 148 <212> PRT <213> Simian adenovirus 28 <400> 26 Met Lys Ala Phe Ala Val Leu Phe Val Leu Ser Leu Ile Lys Thr Glu 1 5 10 15 Phe Arg Pro Ser Tyr Gly Leu Pro Leu Leu Gln Pro Gly Leu Tyr Asn 20 25 30 Thr Ser Gln Thr Leu Arg Ser Ser Gln Lys Thr Gln Ala Leu Pro Leu 35 40 45 Ile Gln Asp Ser Asn Ser Thr Ser Pro Ala Pro Ser Pro Thr Asn Leu 50 55 60 Pro Glu Thr Asn Asn Leu Gly Ala Gln Leu Gln His Arg Phe Ser Arg 65 70 75 80 Ser Leu Leu Ser Ala Asn Thr Thr Thr Pro Lys Thr Gly Gly Glu Leu 85 90 95 Arg Gly Leu Pro Thr Asp Asn Pro Trp Ala Val Ala Gly Phe Val Ala 100 105 110 Leu Gly Val Val Ala Gly Gly Leu Val Leu Ile Leu Cys Tyr Leu Tyr 115 120 125 Thr Pro Cys Cys Ala Tyr Leu Val Ile Leu Cys Cys Trp Phe Lys Lys 130 135 140 Trp Gly Ser Tyr 145 <210> 27 <211> 246 <212> PRT <213> Simian adenovirus 28 <400> 27 Met Ala Ser Gln Gln Arg Glu His Cys Gly Ile Leu His Cys Leu Leu 1 5 10 15 Leu Gly Tyr Met His His His Cys Tyr His Val Cys Glu His Thr Leu 20 25 30 Ala Tyr Ser His Ser Pro Lys Lys Gln Ser Arg Lys Arg Glu Asn Ala 35 40 45 Leu Ile Ile Asn Phe Tyr Leu Gln Lys Met Thr Ser Val Ser Ala Leu 50 55 60 Ile Phe Ala Thr Ile Met Ala Val Gln Gly Gln Ala Val Gln Gly Gln 65 70 75 80 Thr Leu Ile Asn Val His Pro Gly Thr Asn His Thr Leu Val Val Pro 85 90 95 Asn Asn Tyr Ser Asn Ile Glu Trp Gln Trp Phe Thr Asn Asn Val Trp 100 105 110 Tyr Glu Pro Cys Glu His Tyr Ser Leu Phe Ile Cys Asn His Asn Leu 115 120 125 Thr Leu Ile Asn Val Ser Thr Ile His Lys Gly Tyr Tyr Tyr Arg Tyr 130 135 140 Asp Asn His Ser Ile Asp Pro Thr Ile Tyr Leu Val Arg Val Asn Pro 145 150 155 160 Ile Asn Lys Pro Ile Pro Lys Ala Phe Ser Arg Thr Thr Ile Gln Asn 165 170 175 Phe Lys Thr Ala Ile Leu Leu Asn Phe Lys Thr Lys Asn Ile Thr Gly 180 185 190 Asn Ile Leu Pro Thr Thr Pro Thr Glu Lys Asn Thr Pro Asn Ser Ile 195 200 205 Phe Glu Ile Ile Ile Ala Leu Leu Ala Val Gly Ile Thr Ile Ile Leu 210 215 220 Cys Met Ile Ile Tyr Ala His Cys Tyr Lys Lys Ile His His Lys Lys 225 230 235 240 Glu Pro Leu Leu Ser Phe 245 <210> 28 <211> 139 <212> PRT <213> Simian adenovirus 28 <400> 28 Met Leu Gln Gln Phe Leu Gly Ser Phe Lys Thr Met Gln Thr Leu Leu 1 5 10 15 Leu Leu Leu Leu Val Ile His Gln Cys Ala Ser Asn Pro Thr Ser Pro 20 25 30 Thr Lys Leu Asp Leu Arg Lys Cys Lys Phe Gln Glu Pro Trp Lys Phe 35 40 45 Leu Asp Cys Tyr His Glu Thr Ser Asp Phe Pro Thr Tyr Trp Ile Thr 50 55 60 Ile Ile Gly Val Val Asn Leu Val Ser Cys Thr Leu Phe Ser Phe Leu 65 70 75 80 Val Tyr His Leu Phe Asp Phe Gly Trp Asn Ala Leu Asn Ala Leu Thr 85 90 95 Tyr Pro Gln Glu Pro Glu Glu His Ile Pro Leu Gln Asn Ile Gln Pro 100 105 110 Leu Ala Leu Glu Tyr Glu Asn Glu Pro Gln Pro Pro Leu Leu Pro Ala 115 120 125 Ile Ser Tyr Phe Asn Leu Thr Gly Gly Asp Asp 130 135 <210> 29 <211> 1500 <212> DNA <213> Simian adenovirus 28 <220> <221> CDS <222> (574)..(1147) <223> label = Ela <220> <221> CDS <222> (1236)..(1441) <223> label = Ela <400> 29 catcatcaat aatatacctt ataaatggaa cggtgccaac atgcaaatga gcttttgaaa 60 atggagggcg gaaggggatt ggccagcggg ttcaacggtc aaaaggggcg ggccggcgcg 120 gggaggtgac gtatttcgtg tgggaggagt tttgttgcaa gttatcgcgg caaaagtgac 180 gtaaaacgag gtgtggtttg aacacggaag tagacagttt tcccgcgctg actgacagga 240 tatgaggtag ttttgggcgg atgcaagtga aaattcacca ttttcgcgcg aaaactgaat 300 gaggaagtga atttctgagt aatttcgagt ttatgacagg gtggagtatt taccgagggc 360 cgagtagact ttgaccgatt acgtggaggt ttcgattacc gtgtttttca cctaaatttc 420 cgcgtacggt gtcaaagtcc tgtgttttta cgtaggcgtc agctgatcgc tagggtattt 480 aaacctgacg agttccgtca agaggccact cttgagtgcc agcgagaaga gatttctcct 540 ccgcgccgcg agtcagatct ccactttgaa aaa atg aga cac ctg cga ttc ctg 594 Met Arg His Leu Arg Phe Leu 1 5 cct cag gaa atc tcc att gag acc ggg aat gaa ata cta cag ctt gtg 642 Pro Gln Glu Ile Ser Ile Glu Thr Gly Asn Glu Ile Leu Gln Leu Val 10 15 20 gta aat gcc ctg atg gga gac gat ccg gag ccg cct gcg cat ccg ttc 690 Val Asn Ala Leu Met Gly Asp Asp Pro Glu Pro Pro Ala His Pro Phe 25 30 35 gat cct cct acg ctt cat gaa ctg tat gat tta gag gta gat ggg ccg 738 Asp Pro Pro Thr Leu His Glu Leu Tyr Asp Leu Glu Val Asp Gly Pro 40 45 50 55 gag gat cct aac gag gaa gct gtg aat ggt ttt ttt agc gaa tct atg 786 Glu Asp Pro Asn Glu Glu Ala Val Asn Gly Phe Phe Ser Glu Ser Met 60 65 70 cta ttg gct gct aat gaa gga gtg gac ata gac cca cct tct gag acc 834 Leu Leu Ala Ala Asn Glu Gly Val Asp Ile Asp Pro Pro Ser Glu Thr 75 80 85 ctt gat acc cca ggg gtg att gtg gag agc ggc aga ggt ggg aaa aaa 882 Leu Asp Thr Pro Gly Val Ile Val Glu Ser Gly Arg Gly Gly Lys Lys 90 95 100 ttg cct gaa ctt ggt gct gct gaa atg gac ttg cac tgt tat gaa gag 930 Leu Pro Glu Leu Gly Ala Ala Glu Met Asp Leu His Cys Tyr Glu Glu 105 110 115 ggt ttt cct ccg agt gat gaa gag gag gaa aat gtg cag tcg atc cag 978 Gly Phe Pro Pro Ser Asp Glu Glu Glu Glu Asn Val Gln Ser Ile Gln 120 125 130 135 acc gca gcg ggt gag gga atg aaa gct gcc aat gat ggt ttt aag ttg 1026 Thr Ala Ala Gly Glu Gly Met Lys Ala Ala Asn Asp Gly Phe Lys Leu 140 145 150 gac tgc ccg gag ctg cct gga cat ggc tgt aag tct tgt gaa ttt cac 1074 Asp Cys Pro Glu Leu Pro Gly His Gly Cys Lys Ser Cys Glu Phe His 155 160 165 agg aat agt act gga cta aaa gaa ctg ttg tgc tcg ctt tgc tat atg 1122 Arg Asn Ser Thr Gly Leu Lys Glu Leu Leu Cys Ser Leu Cys Tyr Met 170 175 180 aga acg cac tgc cat ttt att tac a gtaagtgtgt ttaacttaaa 1167 Arg Thr His Cys His Phe Ile Tyr 185 190 tttaaaggga cagtgtagca gtttaatgtc tgttgaatgt gggatttatg tctttgtgat 1227 ttttatag gt cct gtg tca gat gct gat gaa tcg cct tct cct gat tca 1276 Ser Pro Val Ser Asp Ala Asp Glu Ser Pro Ser Pro Asp Ser 195 200 205 act acc tca cct cct gaa att cag gcg cca gtc cct gca aac gta tgc 1324 Thr Thr Ser Pro Pro Glu Ile Gln Ala Pro Val Pro Ala Asn Val Cys 210 215 220 aag ccc att cct gtg aag gct aag cct ggg aaa cgc cct gct gtg gat 1372 Lys Pro Ile Pro Val Lys Ala Lys Pro Gly Lys Arg Pro Ala Val Asp 225 230 235 aag ctg gag gac ttg ctt gag ggt ggg gat gga cct ttg gac ttg agt 1420 Lys Leu Glu Asp Leu Leu Glu Gly Gly Asp Gly Pro Leu Asp Leu Ser 240 245 250 acc cgg aaa ctg cca agg caa tgagtgccct gcacctgtgt ttatttaatg 1471 Thr Arg Lys Leu Pro Arg Gln 255 260 tgacgtcagt atttatgtga gagtgccat 1500 <210> 30 <211> 260 <212> PRT <213> Simian adenovirus 28 <400> 30 Met Arg His Leu Arg Phe Leu Pro Gln Glu Ile Ser Ile Glu Thr Gly 1 5 10 15 Asn Glu Ile Leu Gln Leu Val Val Asn Ala Leu Met Gly Asp Asp Pro 20 25 30 Glu Pro Pro Ala His Pro Phe Asp Pro Pro Thr Leu His Glu Leu Tyr 35 40 45 Asp Leu Glu Val Asp Gly Pro Glu Asp Pro Asn Glu Glu Ala Val Asn 50 55 60 Gly Phe Phe Ser Glu Ser Met Leu Leu Ala Ala Asn Glu Gly Val Asp 65 70 75 80 Ile Asp Pro Pro Ser Glu Thr Leu Asp Thr Pro Gly Val Ile Val Glu 85 90 95 Ser Gly Arg Gly Gly Lys Lys Leu Pro Glu Leu Gly Ala Ala Glu Met 100 105 110 Asp Leu His Cys Tyr Glu Glu Gly Phe Pro Pro Ser Asp Glu Glu Glu 115 120 125 Glu Asn Val Gln Ser Ile Gln Thr Ala Ala Gly Glu Gly Met Lys Ala 130 135 140 Ala Asn Asp Gly Phe Lys Leu Asp Cys Pro Glu Leu Pro Gly His Gly 145 150 155 160 Cys Lys Ser Cys Glu Phe His Arg Asn Ser Thr Gly Leu Lys Glu Leu 165 170 175 Leu Cys Ser Leu Cys Tyr Met Arg Thr His Cys His Phe Ile Tyr Ser 180 185 190 Pro Val Ser Asp Ala Asp Glu Ser Pro Ser Pro Asp Ser Thr Thr Ser 195 200 205 Pro Pro Glu Ile Gln Ala Pro Val Pro Ala Asn Val Cys Lys Pro Ile 210 215 220 Pro Val Lys Ala Lys Pro Gly Lys Arg Pro Ala Val Asp Lys Leu Glu 225 230 235 240 Asp Leu Leu Glu Gly Gly Asp Gly Pro Leu Asp Leu Ser Thr Arg Lys 245 250 255 Leu Pro Arg Gln 260 <210> 31 <211> 890 <212> DNA <213> Simian adenovirus 28 <220> <221> CDS <222> (7)..(355) <223> label=33K <220> <221> CDS <222> (525)..(889) <223> label=33K <400> 31 ctcagg atg tcc cag cgc cga gga agc aag aag ttg aag gtg cag ctg 48 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln Leu 1 5 10 ccg ccc cca gag gat atg gag gaa gac tgg gac agt cag gca gag gag 96 Pro Pro Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu Glu 15 20 25 30 gag atg gaa gat tgg gac agc cag gca gag gag gcg gac agc ctg gag 144 Glu Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Ala Asp Ser Leu Glu 35 40 45 gaa gac agt ttg gag gag gaa gac gag gag gca gag gag gtg gaa gaa 192 Glu Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu 50 55 60 gca acc gcc gcc aaa cag ttg tcc tcg gca gcg gag aca agc aag gtc 240 Ala Thr Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Val 65 70 75 cca gac agc agc agc acg gct aca atc tcc gct ccg ggg ggg gcc cag 288 Pro Asp Ser Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Gly Ala Gln 80 85 90 cgg cgt ccc aac agt aga tgg gac gag acc ggg cga ttc ccg aac ccg 336 Arg Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe Pro Asn Pro 95 100 105 110 acc acc gct tcc aag acc g gtaagaagga gcggcaggga tacaagtcct 385 Thr Thr Ala Ser Lys Thr 115 ggcgggggca taagaatgcc atcatctcct gcttgcatga atgcgggggc aacatatcct 445 tcacccggcg ctacctgctc ttccaccacg gggtgaactt cccccgcaat gtcttgcatt 505 actaccgtca cctccacag cc cct act aca acc agc aag tcc cgg cag cct 556 Ala Pro Thr Thr Thr Ser Lys Ser Arg Gln Pro 120 125 cgg cag aga aag aca gca gca gca gca gca gcg ggg acc tcc agc aga 604 Arg Gln Arg Lys Thr Ala Ala Ala Ala Ala Ala Gly Thr Ser Ser Arg 130 135 140 aaa cca gca gca gca gtt aga aaa tcc agt gca gca gga gga gga ctg 652 Lys Pro Ala Ala Ala Val Arg Lys Ser Ser Ala Ala Gly Gly Gly Leu 145 150 155 agg atc aca gcg aac gag cca gcg cag acc cga gag ctg aga aac agg 700 Arg Ile Thr Ala Asn Glu Pro Ala Gln Thr Arg Glu Leu Arg Asn Arg 160 165 170 175 atc ttt cca acc ctc tat gcc atc ttc cag cag agt cgg ggg caa gag 748 Ile Phe Pro Thr Leu Tyr Ala Ile Phe Gln Gln Ser Arg Gly Gln Glu 180 185 190 cag gaa ctg aaa gta aaa aac cga tct ctg cgc tcg ctc acc cga agt 796 Gln Glu Leu Lys Val Lys Asn Arg Ser Leu Arg Ser Leu Thr Arg Ser 195 200 205 tgt ttg tat cac aag agc gaa gac caa ctt cag cgc act ctc gag gac 844 Cys Leu Tyr His Lys Ser Glu Asp Gln Leu Gln Arg Thr Leu Glu Asp 210 215 220 gcc gag gct ctc ttc aac aag tac tgc gcg ctg act ctt aaa gag t 890 Ala Glu Ala Leu Phe Asn Lys Tyr Cys Ala Leu Thr Leu Lys Glu 225 230 235 <210> 32 <211> 238 <212> PRT <213> Simian adenovirus 28 <400> 32 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu Glu Glu Met 20 25 30 Glu Asp Trp Asp Ser Gln Ala Glu Glu Ala Asp Ser Leu Glu Glu Asp 35 40 45 Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu Ala Thr 50 55 60 Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Val Pro Asp 65 70 75 80 Ser Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Gly Ala Gln Arg Arg 85 90 95 Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe Pro Asn Pro Thr Thr 100 105 110 Ala Ser Lys Thr Ala Pro Thr Thr Thr Ser Lys Ser Arg Gln Pro Arg 115 120 125 Gln Arg Lys Thr Ala Ala Ala Ala Ala Ala Gly Thr Ser Ser Arg Lys 130 135 140 Pro Ala Ala Ala Val Arg Lys Ser Ser Ala Ala Gly Gly Gly Leu Arg 145 150 155 160 Ile Thr Ala Asn Glu Pro Ala Gln Thr Arg Glu Leu Arg Asn Arg Ile 165 170 175 Phe Pro Thr Leu Tyr Ala Ile Phe Gln Gln Ser Arg Gly Gln Glu Gln 180 185 190 Glu Leu Lys Val Lys Asn Arg Ser Leu Arg Ser Leu Thr Arg Ser Cys 195 200 205 Leu Tyr His Lys Ser Glu Asp Gln Leu Gln Arg Thr Leu Glu Asp Ala 210 215 220 Glu Ala Leu Phe Asn Lys Tyr Cys Ala Leu Thr Leu Lys Glu 225 230 235 <210> 33 <211> 28 <212> DNA <213> Artificial <220> <223> primer <400> 33 ccatctatcg atgcataatc agcaaacc 28 <210> 34 <211> 26 <212> DNA <213> Artificial <220> <223> primer <400> 34 ctcaaatgga attcaaatgt ttaaag 26 <210> 35 <211> 20 <212> DNA <213> Artificial <220> <223> primer <400> 35 cacaagcccc acaaaattag 20 <210> 36 <211> 20 <212> DNA <213> Artificial <220> <223> primer <400> 36 aagccaagat ctcctacgag 20 <210> 37 <211> 20 <212> DNA <213> Artificial <220> <223> primer <400> 37 tatgaagatg aaagcagttc 20 <210> 38 <211> 20 <212> DNA <213> Artificial <220> <223> primer <400> 38 ctaaaaagac tgcatcaatc 20 <210> 39 <211> 35592 <212> DNA <213> Simian adenovirus 27 <220> <221> repeat_region <222> (1)..(132) <223> label=ITR <220> <221> CDS <222> (1926)..(3410) <223> label=Elb\55K <220> <221> CDS <222> (3506)..(3919) <223> label=pIX <220> <221> misc_feature <222> (3988)..(5609) <223> complement (3988..5321, 5597..5609) label=IVa2 <220> <221> misc_feature <222> (5091)..(13911) <223> complement (5091..8456, 13903..13911) label=pol <220> <221> misc_feature <222> (8462)..(13911) <223> complement (8462..10396, 13903..13911) label=pTP <220> <221> CDS <222> (10921)..(12081) <223> label=52K <220> <221> CDS <222> (12109)..(13869) <223> label=pIIIa <220> <221> CDS <222> (13956)..(15644) <223> label=penton <220> <221> CDS <222> (15651)..(16226) <223> label=pVII <220> <221> CDS <222> (16272)..(17321) <223> label=V <220> <221> CDS <222> (17353)..(17577) <223> label=pX <220> <221> CDS <222> (17655)..(18404) <223> label=pVI <220> <221> CDS <222> (18532)..(21399) <223> label=hexon <220> <221> CDS <222> (21430)..(22056) <223> label=protease <220> <221> misc_feature <222> (22153)..(23700) <223> complement label=DBP <220> <221> CDS <222> (23731)..(26214) <223> label=100K <220> <221> CDS <222> (26861)..(27541) <223> label=pVIII <220> <221> CDS <222> (27544)..(27861) <223> label=E3\12.5K <220> <221> CDS <222> (28243)..(28758) <223> label=E3\gp19K <220> <221> CDS <222> (28798)..(29328) <223> label=E3\CR1-beta <220> <221> CDS <222> (29353)..(29919) <223> label=E3\CR1-gamma <220> <221> CDS <222> (29938)..(30240) <223> label=E3\CR1-delta <220> <221> CDS <222> (30533)..(30967) <223> label=E3\RID-beta <220> <221> CDS <222> (31597)..(32571) <223> label=fiber <220> <221> misc_feature <222> (32615)..(33774) <223> complement (32615..32863, 33586..33774) label=E4\orf6/7 <220> <221> misc_feature <222> (32863)..(33774) <223> complement label=E4\orf6 <220> <221> misc_feature <222> (34058)..(34408) <223> complement label=E4\orf3 <220> <221> misc_feature <222> (34408)..(34794) <223> complement label=E4\orf2 <220> <221> misc_feature <222> (34841)..(35212) <223> complement label=E4\orf1 <220> <221> repeat_region <222> (35461)..(35592) <223> complement label=ITR <400> 39 catcatcaat aatatacctt ataaatggaa cggtgccaac atgcaaatga gcttttgaaa 60 atggagggcg gaaggggatt ggctacaggg ttcaacggtc aaaaggggcg ggccggcgcg 120 gggaggtgac gtgtttagtg tgggaggagt tatgttgcaa gttctcgcgg taaatgtgac 180 gtaaaacgag gtgtgctttg aacacggaag tggacagttt tcccgcgctg actgacagga 240 tatgaggtag ttttgggcgg atgcaagtga aaattctcca ttttcgcgcg aaaactgaat 300 gaggaagtga atttctgagt aatttcgagt ttatgacagg gcggagtatt taccgagggc 360 cgagtagact ttgaccgatt acgtggaggt ttcgattacc gtgtttttca cctaaatttc 420 cgcgtacggt gtcaaagtcc tgtgttttta cgtaggcgtc agctgatcgc tagggtattt 480 aaacctgacg agttccgtca agaggccact cttgagtgcc agcgagaaga gatttctcct 540 ccgcgccgcg agtcagatct ccactttgaa aaaaatgaga cacctgcgat tcctgcctca 600 ggaaatctcc attgagaccg gggatgaaat actgcagttt gtggtaaatg ccctgatggg 660 agacgatccg gagccgcctg cgcagccttt cgatcctcct acgcttcatg aactgtatga 720 tttagaggta gacgggccgg aggaccctaa cgaggaagct gtgaatgggt ttttcagcga 780 ttctatgcta ttagctgcta gtgaaggagt ggacttagac ccaccttctg agacccttga 840 taccccaggg gtggtggtgg aaagcggcgg aggtgggaaa aaattgcctg aacttggtgc 900 tgctgaaatg gatttgcact gttatgaaga gggctttcct ccgagtgatg atgaagatga 960 ggaaaatgtg cagtcgatcc agaccgcagc gggtgaggga ataagagctg ccaatgatgg 1020 ttttaagttg gactacccgg agctgcctgg acatggctgt aagtcttgtg aatttcacag 1080 gaatagtact ggactaaaag aactgttgtg ctcgctttgc tatatgagaa cgcactgcca 1140 ttttatttac agtaagtgtg tttaacttaa atttaaaggg acagtgtagc agtgttaata 1200 actgtgaatg tgggatttat gtttttgctt gtgatttttt ataggtcctg tgtctgatgc 1260 tgatgaatcg ccttctcctg attcaactac ctcacctcct gaaattcagg cgccagtccc 1320 tgcaaacata tgcaagccca ttcctgtgaa ggctaagcct gggaaacgcc ctgctgtgga 1380 taagctggag gacttgcttg agggtgggga tggacctttg gacttgagta cccggaaact 1440 gccaaggcaa tgagtgccct gcacctgtgt ttatttaatg tgacgtcagt atttatgtga 1500 gagtgccatg taataaaatt atgtcagctg ctgagtgttt tattgcttct tgggtgggga 1560 cttggatata taagtaggag cagacctgtg tggttagctc acagcagctt gctgccatcc 1620 atggaggttt gggctatctt ggaagatctc agacagacta ggcaactgct agaaaacgcc 1680 tcggacggag tctctagtct ttggagattc tggttcggtg gtgatctagc taggctagtc 1740 ttcagggtaa aacgggagta tagtgaagaa tttgaaaagt tattggaaga cagtccagga 1800 ctttttgaag ctcttaactt gggccaccag gctcatttta aggagaaggt tttatcagtt 1860 ttagattttt ctacccctgg tagaactgct gcagctgtag ccttccttac ttttatattg 1920 gataa atg gat ccc aca aac cca ctt cag caa ggg ata cgt ttt gga ttt 1970 Met Asp Pro Thr Asn Pro Leu Gln Gln Gly Ile Arg Phe Gly Phe 1 5 10 15 cat agc agc agc ttt gtg gag aac atg gaa ggc tcg cag gct gag gat 2018 His Ser Ser Ser Phe Val Glu Asn Met Glu Gly Ser Gln Ala Glu Asp 20 25 30 aat ctt aga tta ctg gcc agt gca gcc tct ggg cgt agc ggc gat cct 2066 Asn Leu Arg Leu Leu Ala Ser Ala Ala Ser Gly Arg Ser Gly Asp Pro 35 40 45 gag aca ccc acc ggc cat gcc agc ggt tct gga gga gga gca gca gga 2114 Glu Thr Pro Thr Gly His Ala Ser Gly Ser Gly Gly Gly Ala Ala Gly 50 55 60 gga caa ccc gag agc cgg cct gga ccc tcc ggt gga gga ggc gga gga 2162 Gly Gln Pro Glu Ser Arg Pro Gly Pro Ser Gly Gly Gly Gly Gly Gly 65 70 75 gta gct gac ctg ttt cct gaa ctg cga cgg gtg ctt act agg tct acg 2210 Val Ala Asp Leu Phe Pro Glu Leu Arg Arg Val Leu Thr Arg Ser Thr 80 85 90 95 tcc agt gga cag gac agg ggc att aag agg gag agg aat gct agt ggt 2258 Ser Ser Gly Gln Asp Arg Gly Ile Lys Arg Glu Arg Asn Ala Ser Gly 100 105 110 cat aat tca aga act gag ttg gct tta agt tta atg agt cgc agg cgt 2306 His Asn Ser Arg Thr Glu Leu Ala Leu Ser Leu Met Ser Arg Arg Arg 115 120 125 cct gaa act gtt tgg tgg cat gag gtt cag agt gag ggc agg gat gaa 2354 Pro Glu Thr Val Trp Trp His Glu Val Gln Ser Glu Gly Arg Asp Glu 130 135 140 gtt tca ata ttg cag gag aaa tat tcc cta gaa caa ctt aag acc tgt 2402 Val Ser Ile Leu Gln Glu Lys Tyr Ser Leu Glu Gln Leu Lys Thr Cys 145 150 155 tgg ttg gaa cct gaa gat gat tgg gag gtg gcc att agg aat tat gct 2450 Trp Leu Glu Pro Glu Asp Asp Trp Glu Val Ala Ile Arg Asn Tyr Ala 160 165 170 175 aag ata tct ctg agg cct gat aag cag tat aga att acc aag aag att 2498 Lys Ile Ser Leu Arg Pro Asp Lys Gln Tyr Arg Ile Thr Lys Lys Ile 180 185 190 aat atc aga aat gca tgc tac ata tca ggg aat ggg gcc gag gtt ata 2546 Asn Ile Arg Asn Ala Cys Tyr Ile Ser Gly Asn Gly Ala Glu Val Ile 195 200 205 ata gat aca caa gat aaa aca gct ttt aga tgt tgt atg atg ggt atg 2594 Ile Asp Thr Gln Asp Lys Thr Ala Phe Arg Cys Cys Met Met Gly Met 210 215 220 tgg cca ggg gtg gcc ggc atg gag gca gta aca ctt atg aat att agg 2642 Trp Pro Gly Val Ala Gly Met Glu Ala Val Thr Leu Met Asn Ile Arg 225 230 235 ttt agg gga gat ggg tat aat ggg att gtc ttt atg gct aac act aaa 2690 Phe Arg Gly Asp Gly Tyr Asn Gly Ile Val Phe Met Ala Asn Thr Lys 240 245 250 255 tta att ctg cac ggt tgt agc ttt ttt ggg ttt aat aat act tgt gtg 2738 Leu Ile Leu His Gly Cys Ser Phe Phe Gly Phe Asn Asn Thr Cys Val 260 265 270 gaa gca tgg gga cag gtt agt gta aga ggc tgt agt ttt tat gca ggc 2786 Glu Ala Trp Gly Gln Val Ser Val Arg Gly Cys Ser Phe Tyr Ala Gly 275 280 285 tgg att gca cta tca ggc agg acc aag agt cag ttg tct gtg aag aaa 2834 Trp Ile Ala Leu Ser Gly Arg Thr Lys Ser Gln Leu Ser Val Lys Lys 290 295 300 tgc atg ttt gag aga tgt aat ctg ggc ata ctg aat gaa ggt gaa gca 2882 Cys Met Phe Glu Arg Cys Asn Leu Gly Ile Leu Asn Glu Gly Glu Ala 305 310 315 agg gtc cgc cac tgc gct gct aca gaa act ggc tgc ttt att cta ata 2930 Arg Val Arg His Cys Ala Ala Thr Glu Thr Gly Cys Phe Ile Leu Ile 320 325 330 335 aag gga aat gcc agt gtg aag cat aac atg atc tgt gga ccc tca gat 2978 Lys Gly Asn Ala Ser Val Lys His Asn Met Ile Cys Gly Pro Ser Asp 340 345 350 gag agg cct tat cag atg ctg acc tgt gct gga gga cat tgc aat atg 3026 Glu Arg Pro Tyr Gln Met Leu Thr Cys Ala Gly Gly His Cys Asn Met 355 360 365 ctg gct acc gtg cat att gtt tct cat gca cgc aag aaa tgg cct gtg 3074 Leu Ala Thr Val His Ile Val Ser His Ala Arg Lys Lys Trp Pro Val 370 375 380 ttt gag cat aat gtg atg acc aag tgc acc atg cac ata ggt ggt cgc 3122 Phe Glu His Asn Val Met Thr Lys Cys Thr Met His Ile Gly Gly Arg 385 390 395 agg gga atg ttt atg cct tac cag tgt aac ctg aat cat gtg aag gtg 3170 Arg Gly Met Phe Met Pro Tyr Gln Cys Asn Leu Asn His Val Lys Val 400 405 410 415 atg ttg gaa cca gat gcc ttt tcc aga atg agc tta aca gga atc ttt 3218 Met Leu Glu Pro Asp Ala Phe Ser Arg Met Ser Leu Thr Gly Ile Phe 420 425 430 gat atg aat gtg caa cta tgg aag atc ctg aga tat gat gat acc aaa 3266 Asp Met Asn Val Gln Leu Trp Lys Ile Leu Arg Tyr Asp Asp Thr Lys 435 440 445 tcg agg gtg cgc gca tgc gag tgc ggg ggc aag cat gcc agg ttc cag 3314 Ser Arg Val Arg Ala Cys Glu Cys Gly Gly Lys His Ala Arg Phe Gln 450 455 460 ccg gtg tgt gtg gat gtg acg gaa gac ctg aga ccc gat cat ttg gta 3362 Pro Val Cys Val Asp Val Thr Glu Asp Leu Arg Pro Asp His Leu Val 465 470 475 ctt gcc tgc act gga gcg gag ttc ggt tct agt ggg gaa gaa act gac 3410 Leu Ala Cys Thr Gly Ala Glu Phe Gly Ser Ser Gly Glu Glu Thr Asp 480 485 490 495 taaagtgagt agtggggaat gctgtggagg gggcttccag gcgggtaagg tgggcagatt 3470 gggtaaattc tgtctgtttc tgtcttgcag ctgcc atg agt ggg agc gct tct 3523 Met Ser Gly Ser Ala Ser 500 ttt gag ggg gga gtc ttt agc cct tat ctg acg ggg cga ctc cca ccc 3571 Phe Glu Gly Gly Val Phe Ser Pro Tyr Leu Thr Gly Arg Leu Pro Pro 505 510 515 tgg gca gga gtt cgt cag aat gtc atg gga tcc act gtg gat ggg agg 3619 Trp Ala Gly Val Arg Gln Asn Val Met Gly Ser Thr Val Asp Gly Arg 520 525 530 ccc gtc cag ccc gcc aat tcc tca acg ctg acc tat gcc act ttg agc 3667 Pro Val Gln Pro Ala Asn Ser Ser Thr Leu Thr Tyr Ala Thr Leu Ser 535 540 545 tct tca ccc ttg gat gca gcc gca gcc gct gcc gcc tct gct gcc gcc 3715 Ser Ser Pro Leu Asp Ala Ala Ala Ala Ala Ala Ala Ser Ala Ala Ala 550 555 560 565 aac act gtc ctt gga atg ggc tat tat gga agc atc gtt gcc aat tcc 3763 Asn Thr Val Leu Gly Met Gly Tyr Tyr Gly Ser Ile Val Ala Asn Ser 570 575 580 agt tcc tca aat aac cct tcg acc ctg gct gag gac aag cta ctt gtc 3811 Ser Ser Ser Asn Asn Pro Ser Thr Leu Ala Glu Asp Lys Leu Leu Val 585 590 595 ctc ttg gct cag ctc gag gcc ttg acc cag cgc cta ggc gaa ctg tct 3859 Leu Leu Ala Gln Leu Glu Ala Leu Thr Gln Arg Leu Gly Glu Leu Ser 600 605 610 cag cag gtg gcc cag ttg cgc gag caa act gag tct gct gtt gcc aca 3907 Gln Gln Val Ala Gln Leu Arg Glu Gln Thr Glu Ser Ala Val Ala Thr 615 620 625 gca aag tct aaa taaagattcc caaatcaata aataaaggag atccttgttg 3959 Ala Lys Ser Lys 630 attgtaaaac aagtgtaatg aatctttatt tgatttttcg cgcgcggtat gccctggacc 4019 accggtctcg atcattgaga actcggtgga tcttttccag gaccctgtag aggtgggatt 4079 gaatgtttag atacatgggc attaggccgt ctcgggggtg gagatagctc cattgaagag 4139 cctcatgctc cggggtagtg ttataaatca cccagtcata acaaggtcgg agtgcatggt 4199 gttgcacaat atcttttagg agcaggctaa ttgcaacggg gaggccctta gtgtaggtgt 4259 ttacaaatct gttgagctgg gacgggtgca ttcggggtga aattatatgc attttggact 4319 ggatcttgag gttggcaatg ttgccgccta gatcccgtct cgggttcata ttgtgcagga 4379 ccaccaagac agtgtatccg gtgcacttgg gaaatttatc atgcagctta gagggaaaag 4439 catgaaaaaa tttcgagacg cctttgtgtc cgcccagatt ctccatgcac tcatccataa 4499 taatagcgat ggggccgtgg gcggcggcgc gggcaaacac gttccggggg tctgacacat 4559 catagttatg ctcctgagtc aggtcatcat aagccatttt aataaacttg gggcggaggg 4619 tgccagattg ggggatgaaa gttccctcgg gccctggagc atagtttccc tcacatattt 4679 gcatttccca ggctttcagt tcagaggggg ggatcatgtc cacctgcggg gctataaaaa 4739 ataccgtttc tggagcgggg gtgattaact gggatgagag caaattcctg agcagctgag 4799 acttgccaca cccagtggga ccgtaaatga ccccgattac gggttgcaga tggtagttta 4859 gggagcggca gctgccgtcc tcccggagca ggggggccac ttcgttcatc atttccctta 4919 catggatatt ttcccgcacc aagtccgtta ggaggcgctc tccccccagg gatagaagct 4979 cctggagcga ggagaagttt ttcagcggct tcagcccgtc agccatgggc attttggaga 5039 gagtctgttg caagagctcg agccggtccc agagctcggt gatgtgttct atggcatctc 5099 gatccagcag acctcctcgt ttcgcgggtt ggggcggctc ctggagtatg gtatcagacg 5159 atgggcgtcc agcgctgcca gggtccgatc tttccagggt cgcagcgtcc gagtcagggt 5219 tgtttccgtc acggtgaatg ggtgcgcgcc tggttgggcg cttgcgaggg tgcgcttcag 5279 gctcatcctg ctggtcgaga accgctgccg atcggcgccc tgcatgtcgg ccaggtagca 5339 gtttaccatg agttcgtagt tgagcgcctc ggcagcgtgg cctttggcgc ggagcttacc 5399 tttggaagtt ttctggcagg cggggcagta cagacacttg agggcataca gtttaggagc 5459 gaggaagatg gattcggggg agtatgcatc cgcgccacag gaggcgcaga cggtttcgca 5519 ctccacgagc caggtcagat ccggctcatc ggggtcaaaa acaagttttc ctccatgttt 5579 tttgatgcgt ttcttacctt tggtttccat gagttcgtgt ccccgctggg tgacaaagag 5639 gctgtccgtg tccccgtaga ccgattttat gggcctgtcc tggagcggag tgcctcggtc 5699 ctcttcgtag aggaactcgg accactctga tacaaaggcg cgcgtccagg ccagcacaaa 5759 agaggccacg tgggaggggt agcggtcgtt gtcaaccagg gggtccacct tctccacggt 5819 atgtaaacac atgtccccct cctccacatc caggaatgtg attggtttgt aagtgtatgc 5879 cacgtgacca ggggtccccg ccgggggggt ataaaagggg gcgggtctct gctcgtcctc 5939 actgtcttcc ggatcgctgt ccaggagcgc cagctgttgg ggtaggtatt ccctctcgaa 5999 ggcgggcata acctctgcac tcaggttgtc agtttctagg aacgaggagg atttgatatt 6059 gacagtgcca gccgagatgc ctttcatgag actctcgtcc atttggtcag aaaacacaat 6119 cttcttgttg tccagtttgg tggcaaagga tccatagagg gcattggata ggagcttggc 6179 tatggagcgc atggtttggt tcttttcctt gtcagcccgc tccttggcag caatgttgag 6239 ctggacatac tcgcgcgcca gacacttcca ttcagggaag atggttgtta gttcatctgg 6299 cacgattctg actcgccagc ccctattatg cagggttatc agatccacac tggtggtcac 6359 ttcgcctctg aggggctcgt tggtccagca gagtcgaccc ccttttctcg aacagaaagg 6419 ggggaggggg tctagcatga gttcatcagg ggggtctgca tccatagtga agattcctgg 6479 gagtaggtcc ttgtcaaaat agctgatggg ggtggggtca tccaaagcca tctgccattc 6539 tcgagctgcc agcgcgcgct cataggggtt gagaggggtg ccccagggca tggggtgggt 6599 gagtgcagag gcatacatgc cacagatgtc atagacatag aggggctctt caagaatgcc 6659 aatgtaagtg ggataacagc gcccccctct gatgcttgct cgcacatagt catagagttc 6719 atgcgagggg gcgagcagac ccgagcccaa attagtgcga ttgggttttt ctgctctgta 6779 gacgatctgg cgaaagatgg catgtgaatt tgaagagatg gtgggtcttt gaaagatgtt 6839 aaaatgggca tgaggtagac ctacagagtc cctgatgaag tgggcatagg actcttgcag 6899 cttggccacc agttcggcag tgacaaggac atccagggca cagtagtcaa gggtctcttg 6959 gatgatgtca taacctggtt ggtttttctt ttcccacagc tcgcggttga gaaggtattc 7019 ttcgcgatcc ttccagtact cttcaagggg aaacccgtct ttgtctgcac ggtaagagcc 7079 cagcatgtag aactgattaa ctgccttgta gggacagcat cccttctcca cggggagaga 7139 gtatgcttgg gctgccttgc gcagtgaggt atgagtgagg gcgaaagtgt ccctgaccat 7199 gactttgagg aactggtact tgaaatcgat gtcatcacag gccccctgtt cccagagttg 7259 gaagtccgcc cgctttttgt aggcggggtt gggcaaagcg aaagtaacat cattgaagag 7319 aattttgccg gccctgggca tgaaattgcg ggtgatgcgg aaaggccggg gcacctctgc 7379 tcggttattg atgacctgag cggctaggac gatctcgtca aagccattga tgttgtgccc 7439 cacaatgtaa agttctatga atcgcggggt gcccctgaca tgaggcagct tcttgagttc 7499 ttcaaaagtg aggtctgtag ggtcagagag agcatagtgt tcgagggccc attcgtgcag 7559 gtgagggttt gcattgagga aggaggacca gagatccact gccagtgctg tttgtaactg 7619 gtcccggtac tggcgaaaat gctggccgac tgccatcttt tctggggtga tacagtagaa 7679 ggttttgggg tcctgctgcc agcgatccca cttgagtttc atggcgaggt cgtaggcgat 7739 attgacgagc cgctcgtccc ccgagagttt catgaccagc atgaagggta ttagctgctt 7799 gccaaaggac cccatccagg tgtaggtttc cacatcgtag gtgaggaaga gcctttctgt 7859 gcgaggatga gagccgatcg ggaagaactg gatctcctgc caccagttgg aggaatggct 7919 gttgatgtga tggaagtaga actccctgcg gcgcgccgag cattcatgct tgtgcttgta 7979 cagacggccg cagtactcgc agcgcttcac gggatgcacc tcatgaatga gttgtacctg 8039 gcttcctttg acaagaaatt tcagtgggaa gttgaggcct ggcgcttgta cctcgcgctc 8099 tactacatta tctgcatcgg cctggccatc ttctgtctcg atggtggtca tgctgacgag 8159 cccccgcggg aggcaagtcc agacctcggc gcgggagggg cggagctcga ggacgagagc 8219 gcgcaggccg gagctgtcca gggtcctgag acgctgcgga gtcaggttag taggtagtgt 8279 caggagatta acttgcatga tcttttcgag ggcatgcggg aggttcagat ggtacttgat 8339 ttccacgggt ccgttggtgg agatgtcgat ggcttgcagg gtcccgtgcc ccttgggtgc 8399 caccactgtg cccttgtttt tccttttggg cggaggtggc tctgttgctt cttgcatgtt 8459 cagaagcggt gacgagggcg cgcgccgggc ggtaggggcg gctcgggccc cggcggcatg 8519 gctggtagag gcacgtcggc gccgcgcgcg ggtaggttct ggtactgcgc cctgagaaga 8579 cttgcgtgcg cgacgacgcg gcggttgacg tcctggatct gacgcctctg ggtgaaagct 8639 accggacccg tgagcttgaa cctgaaagag agttcaacag aatcaatttc ggtatcgttg 8699 acggcggctt gcctcaggat ctcttgcacg tcgcccgagt tgtcctggta ggcgatctcg 8759 gccatgaact gctcgatttc ttcctcctga agatctccgc ggcccgctct ctcgacagtg 8819 gccgcgaggt cgttggagat gcgacccatg agttgagaga atgcattcat gcccgcctcg 8879 ttccagacgc ggctgtagac cacggccccc tcgggatctc tcgcgcgcat gaccacctgg 8939 gcgaggttga gctccacgtg gcgggtgaag accgcatagt tgcataggcg ctggaagagg 8999 tagttgagtg tggtggcgat gtgctcggtg acgaagaaat acatgatcca tcgtctcagc 9059 ggcatttcgc tgacatcgcc cagggcttcc aagcgctcca tggcctcgta gaagtccaca 9119 gcgaagttga aaaactggga gttgcgcgcg gacacggtca actcctcctc cagaagacgg 9179 atgagttcgg cgatggtggc gcgcacctcg cgctcgaaag cccccgggat ttcttcctcc 9239 tcctcttctt ctatctcttc ttccactaat atctcttctt cctcttcagg cgggggcgga 9299 ggaggagggg gcgcgcggcg acgccggcgg cgcacgggca gacggtcgat gaatctttca 9359 atgacctctc cgcggcggcg gcgcatggtc tcggtgacgg cgcggccgtt ctccctgggt 9419 ctcagagtga agacgcctcc gcgcatctcc ctgaagtggt gactgggggg ttctccgttg 9479 ggcagggaca gggcgctgat gatgcatttt atcaattgcc ccgtagggac tccgtgcaag 9539 gacctgatcg tctgaagatc cacgggatct gaaaaccttt cgacgaaagc gtctaaccag 9599 tcgcaatcgc aaggtaggct gagcactgtt tcttgcggga gggggcggct agacgctcgg 9659 tcggggtttt ctcttccttc cccttcttca tcatctcggg agggtgagac gatgctgctg 9719 gtgatgaaat taaaataggc agttctgaga cggcggatgg tggcgaggag caccaggtct 9779 ttgggtccgg cttgctggat gcgcaggcga tcggccattc cccaagcatt gtcctggcat 9839 ctggccagat ctttatagta gtcttgcatg agtcgctcca cgggcacttc ttcttcgccc 9899 gctctgccat gcatgcgcgt gagcccgaac ccgcgcatgg gctggacaag tgccaggtcc 9959 gctacgaccc tttcggcgag gatggcttgc tgcacctggg tgagggtggc ttggaagtcg 10019 tcaaagtcca cgaagcgatg gtaggctccg gtgttaatgg tgtaggagca gttggccatg 10079 actgaccagt tgactgtctg gtgccccggg cgcacgagct cggtgtactt gaggcgcgag 10139 taggcgcggg tgtcaaagat gtaatcgttg caggtgcgca ccaggtactg gtagccgatg 10199 agaaagtgcg gcggtggctg gcggtagagg ggccatcgct ctgtagccgg ggcaccgggg 10259 gcgaggtctt ccagcatgag gcggtggtat ccgtagatgt acctggacat ccaggtgatc 10319 ccggaggcgg tggtggacgc ccgcgggaac tcgcgcactc ggttccagat gttgcgcagc 10379 ggcatgaagt agttcatggt aggcacggtc tgaccagtga ggcgggcgca gtcattgatg 10439 ctctatagac acggagaaaa cgaaagcgat gagcggctcg cctccgtggc ctggaggaac 10499 gtgaacgggt tgggtcgcgg tgtaccccgg ttcgagacca aagccaagcg agctcaactc 10559 gagccggccg gagccgcggc taacgtggta ttggcgatcc cgtctcgacc cagccgacga 10619 atatccagga tacggagtcg agtcgttttg ctgcttgttg ctttttcctg gacgggagcc 10679 agtgccgcgt caagctttag aacgctcagt tcacggggcc gggagtggct cgcgcccgta 10739 gtctggagaa tcaatcgcca gggttgcgtt gcggtgtgcc ccggttcgag ccttagcgcg 10799 gcccggatcg gccggtttcc gcggcaagcg agggtttggc agccccgtca tttctaagac 10859 cccgccagcc gacttctcca gtttacggga gcgagccctc ttttttgttt ttgtcgccca 10919 g atg cat ccc gtg ctg cga cag atg cgc ccc cag caa cag gcc ccc tct 10968 Met His Pro Val Leu Arg Gln Met Arg Pro Gln Gln Gln Ala Pro Ser 635 640 645 cag caa cag cag cag cca caa aaa gct ctt cct gct cct gca act act 11016 Gln Gln Gln Gln Gln Pro Gln Lys Ala Leu Pro Ala Pro Ala Thr Thr 650 655 660 665 gca gcc gca gcc gtg tgc ggc gcg gga cag ccc gcc tat gat ctg gac 11064 Ala Ala Ala Ala Val Cys Gly Ala Gly Gln Pro Ala Tyr Asp Leu Asp 670 675 680 ttg gaa gag ggc gag gga ctg gca cgc ctg ggt gca cca tcg ccc gag 11112 Leu Glu Glu Gly Glu Gly Leu Ala Arg Leu Gly Ala Pro Ser Pro Glu 685 690 695 cga cac ccg cgg gtg caa ctg aaa aag gac tct cgc gag gcg tac gtg 11160 Arg His Pro Arg Val Gln Leu Lys Lys Asp Ser Arg Glu Ala Tyr Val 700 705 710 ccc cag cat aac ctg ttc agg gac agg agc ggc gag gag ccc gag gag 11208 Pro Gln His Asn Leu Phe Arg Asp Arg Ser Gly Glu Glu Pro Glu Glu 715 720 725 atg cga gcc tct cgc ttt aac gcg ggt cgc gag ctg cgc cac ggt ctg 11256 Met Arg Ala Ser Arg Phe Asn Ala Gly Arg Glu Leu Arg His Gly Leu 730 735 740 745 gac cga aga cgg ctg ctg cgg gac gag gat ttc gag gtc gat gaa gtg 11304 Asp Arg Arg Arg Leu Leu Arg Asp Glu Asp Phe Glu Val Asp Glu Val 750 755 760 aca ggg atc agc ccc gct agg gca cat gtg gcc gcg gcc aac ctc gtc 11352 Thr Gly Ile Ser Pro Ala Arg Ala His Val Ala Ala Ala Asn Leu Val 765 770 775 tcg gcc tac gag cag acc gtg aag gag gag cgc aac ttc caa aaa tca 11400 Ser Ala Tyr Glu Gln Thr Val Lys Glu Glu Arg Asn Phe Gln Lys Ser 780 785 790 ttc aac aac cat gtg cgc acc ctg atc gcc cgt gag gaa gtg acc ctg 11448 Phe Asn Asn His Val Arg Thr Leu Ile Ala Arg Glu Glu Val Thr Leu 795 800 805 ggt ctg atg cac ctg tgg gac ctg atg gaa gct atc acc cag aac ccc 11496 Gly Leu Met His Leu Trp Asp Leu Met Glu Ala Ile Thr Gln Asn Pro 810 815 820 825 act agc aaa ccc ctg acc gct cag ctg ttt ctg gta gtg caa cac agc 11544 Thr Ser Lys Pro Leu Thr Ala Gln Leu Phe Leu Val Val Gln His Ser 830 835 840 agg gac aat gag gca ttc agg gag gcg ctg ctg aac atc acc gag ccc 11592 Arg Asp Asn Glu Ala Phe Arg Glu Ala Leu Leu Asn Ile Thr Glu Pro 845 850 855 gag ggg aga tgg ttg tat gat cta atc aat atc ctg caa agt att ata 11640 Glu Gly Arg Trp Leu Tyr Asp Leu Ile Asn Ile Leu Gln Ser Ile Ile 860 865 870 gtg cag gaa cgc agc cta ggt ctg gct gag aaa gtg gca gcc atc aac 11688 Val Gln Glu Arg Ser Leu Gly Leu Ala Glu Lys Val Ala Ala Ile Asn 875 880 885 tac tct gtc ttg agc ctg ggc aag tac tac gct cgc aag atc tac aag 11736 Tyr Ser Val Leu Ser Leu Gly Lys Tyr Tyr Ala Arg Lys Ile Tyr Lys 890 895 900 905 acc ccc tac gtg ccc ata gac aag gag gtg aag ata gat ggg ttt tac 11784 Thr Pro Tyr Val Pro Ile Asp Lys Glu Val Lys Ile Asp Gly Phe Tyr 910 915 920 atg cgc atg act ctc aag gtg ctg act ctc agt gac gat ctg ggg gtg 11832 Met Arg Met Thr Leu Lys Val Leu Thr Leu Ser Asp Asp Leu Gly Val 925 930 935 tac cgc aac gac agg atg cac cgc gcg gtg agc gcc agc agg agg cgc 11880 Tyr Arg Asn Asp Arg Met His Arg Ala Val Ser Ala Ser Arg Arg Arg 940 945 950 gag ctg agc gac aga gaa ctt atg cac agc ttg caa aga gct ctg acg 11928 Glu Leu Ser Asp Arg Glu Leu Met His Ser Leu Gln Arg Ala Leu Thr 955 960 965 ggg gca ggg acc gat ggg gag aac tac ttt gac atg ggg gca gac ttg 11976 Gly Ala Gly Thr Asp Gly Glu Asn Tyr Phe Asp Met Gly Ala Asp Leu 970 975 980 985 caa tgg caa cct agc cgc cgg gcc ctg gac gcg gca ggg tgt gag ctt 12024 Gln Trp Gln Pro Ser Arg Arg Ala Leu Asp Ala Ala Gly Cys Glu Leu 990 995 1000 cct tac gta gaa gag gtg gat gaa ggc gag gag gag gag ggc gag 12069 Pro Tyr Val Glu Glu Val Asp Glu Gly Glu Glu Glu Glu Gly Glu 1005 1010 1015 tac ctg gaa gac tgatggcgcg acccgtattt ttgctag atg gaa cag cag 12120 Tyr Leu Glu Asp Met Glu Gln Gln 1020 gca ccg gac ccc gca atg cgg gcg gcg ctg cag agc cag ccg tcc 12165 Ala Pro Asp Pro Ala Met Arg Ala Ala Leu Gln Ser Gln Pro Ser 1025 1030 1035 ggc att aac tcc tcg gac gat tgg acc cag gcc atg caa cgc atc 12210 Gly Ile Asn Ser Ser Asp Asp Trp Thr Gln Ala Met Gln Arg Ile 1040 1045 1050 atg gcg ctg acg acc cgc aac ccc gaa gcc ttt aga cag caa ccc 12255 Met Ala Leu Thr Thr Arg Asn Pro Glu Ala Phe Arg Gln Gln Pro 1055 1060 1065 cag gcc aat cgc ctt tcg gcc atc ctg gag gcc gta gtt cct tcc 12300 Gln Ala Asn Arg Leu Ser Ala Ile Leu Glu Ala Val Val Pro Ser 1070 1075 1080 cgc tcc aac ccc acc cac gag aag gtc ctg gcc atc gtg aac gcg 12345 Arg Ser Asn Pro Thr His Glu Lys Val Leu Ala Ile Val Asn Ala 1085 1090 1095 ctg gtg gag aac aag gcc atc cgt ccc gat gag gcc ggg ctg gta 12390 Leu Val Glu Asn Lys Ala Ile Arg Pro Asp Glu Ala Gly Leu Val 1100 1105 1110 tac aat gcc ctc ttg gag cgc gtg gcc cgc tac aac agc agc aac 12435 Tyr Asn Ala Leu Leu Glu Arg Val Ala Arg Tyr Asn Ser Ser Asn 1115 1120 1125 gtg cag acc aac ctg gac cgg atg gtg acc gat gtg cgc gag gcc 12480 Val Gln Thr Asn Leu Asp Arg Met Val Thr Asp Val Arg Glu Ala 1130 1135 1140 gtg tct cag cgc gag cgg ttc cgg cgc gat gcc aac ttg ggg tcg 12525 Val Ser Gln Arg Glu Arg Phe Arg Arg Asp Ala Asn Leu Gly Ser 1145 1150 1155 ctg gtg gcg ctg aac gcc ttc ctc agc acc cag cct gcc aac gtg 12570 Leu Val Ala Leu Asn Ala Phe Leu Ser Thr Gln Pro Ala Asn Val 1160 1165 1170 ccc cgc ggc cag caa gac tat aca aac ttt cta agt gca ctg aga 12615 Pro Arg Gly Gln Gln Asp Tyr Thr Asn Phe Leu Ser Ala Leu Arg 1175 1180 1185 ctc atg gta acc gaa gtc cct cag agc gag gtt tac cag tcc ggt 12660 Leu Met Val Thr Glu Val Pro Gln Ser Glu Val Tyr Gln Ser Gly 1190 1195 1200 cca gac tac ttc ttc cag acc agc aga cag ggc ttg cag aca gtg 12705 Pro Asp Tyr Phe Phe Gln Thr Ser Arg Gln Gly Leu Gln Thr Val 1205 1210 1215 aac ctg agc cag gct ttc aag aac ctc aga ggc ctg tgg gga gtg 12750 Asn Leu Ser Gln Ala Phe Lys Asn Leu Arg Gly Leu Trp Gly Val 1220 1225 1230 cac gcc ccc gta gga gat cgc gcg acc gtg tct agc ttg ctg acc 12795 His Ala Pro Val Gly Asp Arg Ala Thr Val Ser Ser Leu Leu Thr 1235 1240 1245 ccc aac tcc cgc cta ctg ctg ctg ctg gta tcc ccc ttc act gac 12840 Pro Asn Ser Arg Leu Leu Leu Leu Leu Val Ser Pro Phe Thr Asp 1250 1255 1260 agt ggt agc atc gac cgc aac tct tac ttg ggc tac ctg ctg aac 12885 Ser Gly Ser Ile Asp Arg Asn Ser Tyr Leu Gly Tyr Leu Leu Asn 1265 1270 1275 ttg tat cgc gag gcc ata ggg cag agc cag gtg gac gag cag acc 12930 Leu Tyr Arg Glu Ala Ile Gly Gln Ser Gln Val Asp Glu Gln Thr 1280 1285 1290 tat caa gaa atc acc caa gtg agc cgc gcc ctg ggt cag gaa gac 12975 Tyr Gln Glu Ile Thr Gln Val Ser Arg Ala Leu Gly Gln Glu Asp 1295 1300 1305 acg ggc agc ttg gaa gcc act ttg aac ttc ttg ctg acc aac cga 13020 Thr Gly Ser Leu Glu Ala Thr Leu Asn Phe Leu Leu Thr Asn Arg 1310 1315 1320 tcg cag aag atc cct cct cag tat gcg ctt acc gcg gag gag gaa 13065 Ser Gln Lys Ile Pro Pro Gln Tyr Ala Leu Thr Ala Glu Glu Glu 1325 1330 1335 cgg att ctg aga tat gtg cag cag agc gtg gga ctg ttc ctg atg 13110 Arg Ile Leu Arg Tyr Val Gln Gln Ser Val Gly Leu Phe Leu Met 1340 1345 1350 cag gag ggg gca acc cct acc gcc gcg ttg gac atg aca gcg cga 13155 Gln Glu Gly Ala Thr Pro Thr Ala Ala Leu Asp Met Thr Ala Arg 1355 1360 1365 aac atg gag ccc agc atg tat gcc agt aac cgg cct ttc att aac 13200 Asn Met Glu Pro Ser Met Tyr Ala Ser Asn Arg Pro Phe Ile Asn 1370 1375 1380 aaa ctg ctg gat tac ctg cac agg gca gcc gct atg aac tct gat 13245 Lys Leu Leu Asp Tyr Leu His Arg Ala Ala Ala Met Asn Ser Asp 1385 1390 1395 tat ttt acc aat gct att ctc aac ccc cac tgg ctg cct ccg cct 13290 Tyr Phe Thr Asn Ala Ile Leu Asn Pro His Trp Leu Pro Pro Pro 1400 1405 1410 gga ttt tac acg ggc gag tat gat atg ccc gac ccc aat gac ggg 13335 Gly Phe Tyr Thr Gly Glu Tyr Asp Met Pro Asp Pro Asn Asp Gly 1415 1420 1425 ttt ctg tgg gac gat gtg gac agc agc ata ttc tcc ccg cct cct 13380 Phe Leu Trp Asp Asp Val Asp Ser Ser Ile Phe Ser Pro Pro Pro 1430 1435 1440 ggt tat aac act tgg aag aag gaa ggg ggc gat aga aga cac tct 13425 Gly Tyr Asn Thr Trp Lys Lys Glu Gly Gly Asp Arg Arg His Ser 1445 1450 1455 tcc gtg tcg ctg tcc ggg tcg agg ggt gct gcc gct gcg gtg ccc 13470 Ser Val Ser Leu Ser Gly Ser Arg Gly Ala Ala Ala Ala Val Pro 1460 1465 1470 gag gct gca agt cct ttc cct agc ctg ccc ttt tct ctg aac agt 13515 Glu Ala Ala Ser Pro Phe Pro Ser Leu Pro Phe Ser Leu Asn Ser 1475 1480 1485 gtg cgc agc agt gaa ctg ggg aga ata acc cgc ccg cgt ttg atg 13560 Val Arg Ser Ser Glu Leu Gly Arg Ile Thr Arg Pro Arg Leu Met 1490 1495 1500 ggc gag gag gaa tac ttg aac gac tct ttg ctt aga ccc gag agg 13605 Gly Glu Glu Glu Tyr Leu Asn Asp Ser Leu Leu Arg Pro Glu Arg 1505 1510 1515 gaa aag aac ttc ccc aac aat ggg ata gag agc ctg gtg gat aag 13650 Glu Lys Asn Phe Pro Asn Asn Gly Ile Glu Ser Leu Val Asp Lys 1520 1525 1530 atg agc aga tgg aaa acc tat gca cag gat cac aaa gac gag cct 13695 Met Ser Arg Trp Lys Thr Tyr Ala Gln Asp His Lys Asp Glu Pro 1535 1540 1545 agg atc ttg ggg gct gct agc ggg acg acc cgt aga cgc cag cgc 13740 Arg Ile Leu Gly Ala Ala Ser Gly Thr Thr Arg Arg Arg Gln Arg 1550 1555 1560 cat gac aga cag agg ggt ctt gta tgg gac gat gag gac tcg gcc 13785 His Asp Arg Gln Arg Gly Leu Val Trp Asp Asp Glu Asp Ser Ala 1565 1570 1575 gat gac agc agc gtg ttg gac ttg ggt ggg aga gga ggg ggc aac 13830 Asp Asp Ser Ser Val Leu Asp Leu Gly Gly Arg Gly Gly Gly Asn 1580 1585 1590 ccg ttc gct cat ctg cgc ccg cac ttt ggg cgc atg ttg taaaagtgaa 13879 Pro Phe Ala His Leu Arg Pro His Phe Gly Arg Met Leu 1595 1600 1605 agtaaaataa aaaggcaact caccaaggcc atggcgacga gcgtgcgttc gttcttttct 13939 gttatctgtg tctagt atg atg agg cga gcc gtg cta ggc gga gcg gtg 13988 Met Met Arg Arg Ala Val Leu Gly Gly Ala Val 1610 1615 gtg tat ccg gag ggt cct cct cct tcg tac gag agc gtg atg cag 14033 Val Tyr Pro Glu Gly Pro Pro Pro Ser Tyr Glu Ser Val Met Gln 1620 1625 1630 cag cag gcg gcg gcg gtg atg cag ccc tcg ctg gag gct ccc ttt 14078 Gln Gln Ala Ala Ala Val Met Gln Pro Ser Leu Glu Ala Pro Phe 1635 1640 1645 gta ccc ccg cgg tac ctg gcg cct aca gag ggg aga aac agc att 14123 Val Pro Pro Arg Tyr Leu Ala Pro Thr Glu Gly Arg Asn Ser Ile 1650 1655 1660 cgt tac tcg gag ctg gca ccc cag tac gat acc acc agg ttg tat 14168 Arg Tyr Ser Glu Leu Ala Pro Gln Tyr Asp Thr Thr Arg Leu Tyr 1665 1670 1675 ctg gtg gac aac aag tcg gcg gac atc gcc tca ttg aac tat cag 14213 Leu Val Asp Asn Lys Ser Ala Asp Ile Ala Ser Leu Asn Tyr Gln 1680 1685 1690 aac gac cac agc aac ttc ctg acc acg gtg gtg cag aac aat gac 14258 Asn Asp His Ser Asn Phe Leu Thr Thr Val Val Gln Asn Asn Asp 1695 1700 1705 ttt acc ccc acg gag gcc agc acc cag acc atc aac ttt gac gag 14303 Phe Thr Pro Thr Glu Ala Ser Thr Gln Thr Ile Asn Phe Asp Glu 1710 1715 1720 cgg tcg cgg tgg ggc ggt cag ctg aag acc atc atg cac acc aac 14348 Arg Ser Arg Trp Gly Gly Gln Leu Lys Thr Ile Met His Thr Asn 1725 1730 1735 atg ccc aac gtg aac gag tac atg ttc agc aac aag ttc aag gct 14393 Met Pro Asn Val Asn Glu Tyr Met Phe Ser Asn Lys Phe Lys Ala 1740 1745 1750 cgg gtg atg gtg tct aga aag gct cct gaa ggt gtt aca gta gct 14438 Arg Val Met Val Ser Arg Lys Ala Pro Glu Gly Val Thr Val Ala 1755 1760 1765 gat aat tat gac cac aag cag gat att ctg gag tat gag tgg ttc 14483 Asp Asn Tyr Asp His Lys Gln Asp Ile Leu Glu Tyr Glu Trp Phe 1770 1775 1780 gag ttc act tta cca gaa ggc aac ttc tca gcc act atg acc atc 14528 Glu Phe Thr Leu Pro Glu Gly Asn Phe Ser Ala Thr Met Thr Ile 1785 1790 1795 gac ctg atg aac aat gcc atc ata gac aac tac ttg gaa gtt ggc 14573 Asp Leu Met Asn Asn Ala Ile Ile Asp Asn Tyr Leu Glu Val Gly 1800 1805 1810 aga cag aat gga gtg atg gag agt gac att ggt gtc aag ttt gac 14618 Arg Gln Asn Gly Val Met Glu Ser Asp Ile Gly Val Lys Phe Asp 1815 1820 1825 acc agg aac ttc aga ctg ggc tgg gac ccc aaa act aag ttg att 14663 Thr Arg Asn Phe Arg Leu Gly Trp Asp Pro Lys Thr Lys Leu Ile 1830 1835 1840 atg cca ggg gtc tat act tat gaa gca ttc cat cct gac att gtg 14708 Met Pro Gly Val Tyr Thr Tyr Glu Ala Phe His Pro Asp Ile Val 1845 1850 1855 cta ctg cct ggc tgc ggg gtg gac ttt act gag agt cgc ctt agc 14753 Leu Leu Pro Gly Cys Gly Val Asp Phe Thr Glu Ser Arg Leu Ser 1860 1865 1870 aac ttg ctt ggc atc agg aag aga cac cca ttc cag gag ggt ttc 14798 Asn Leu Leu Gly Ile Arg Lys Arg His Pro Phe Gln Glu Gly Phe 1875 1880 1885 aaa ata ttg tat gag gat cta gaa ggg ggt aat atc ccc gcc ctc 14843 Lys Ile Leu Tyr Glu Asp Leu Glu Gly Gly Asn Ile Pro Ala Leu 1890 1895 1900 ttg gac gta gaa gca tat gaa aaa agt aag aaa gaa caa gaa gct 14888 Leu Asp Val Glu Ala Tyr Glu Lys Ser Lys Lys Glu Gln Glu Ala 1905 1910 1915 aaa aca gaa gct gct aaa gct gct gct gtt gct aaa gcc aac ata 14933 Lys Thr Glu Ala Ala Lys Ala Ala Ala Val Ala Lys Ala Asn Ile 1920 1925 1930 gtt gcc agc gac ccc gta agg gta gct aat gct gaa gaa gtc aga 14978 Val Ala Ser Asp Pro Val Arg Val Ala Asn Ala Glu Glu Val Arg 1935 1940 1945 gga gac aac tat act gcc tca gct gtt gcg act gaa gaa tca cta 15023 Gly Asp Asn Tyr Thr Ala Ser Ala Val Ala Thr Glu Glu Ser Leu 1950 1955 1960 ttg gct gct gtg gct gaa aac gaa act aca gaa aca aaa ctc act 15068 Leu Ala Ala Val Ala Glu Asn Glu Thr Thr Glu Thr Lys Leu Thr 1965 1970 1975 atc caa cca gta gaa aag gat agt aag agt aga agc tat aat gtc 15113 Ile Gln Pro Val Glu Lys Asp Ser Lys Ser Arg Ser Tyr Asn Val 1980 1985 1990 ttg gac gat aaa atc aac aca gca tat cgc agt tgg tac ttg tca 15158 Leu Asp Asp Lys Ile Asn Thr Ala Tyr Arg Ser Trp Tyr Leu Ser 1995 2000 2005 tac aac tat gga gac cct gaa aaa gga gtc cgt tcc tgg aca ctg 15203 Tyr Asn Tyr Gly Asp Pro Glu Lys Gly Val Arg Ser Trp Thr Leu 2010 2015 2020 ctt acc act tca gat gtc aca tgc ggg gcg gaa cag gtc tac tgg 15248 Leu Thr Thr Ser Asp Val Thr Cys Gly Ala Glu Gln Val Tyr Trp 2025 2030 2035 tcg ctc cca gac atg atg cag gac ccc gtt acc ttt cgc tcc acg 15293 Ser Leu Pro Asp Met Met Gln Asp Pro Val Thr Phe Arg Ser Thr 2040 2045 2050 aga caa gtc agc aac tat cca gtg gtg ggc gca gag ctc atg ccc 15338 Arg Gln Val Ser Asn Tyr Pro Val Val Gly Ala Glu Leu Met Pro 2055 2060 2065 gtc ttc tca aag agt ttc tac aac gag caa gcc gtg tac tcc cag 15383 Val Phe Ser Lys Ser Phe Tyr Asn Glu Gln Ala Val Tyr Ser Gln 2070 2075 2080 cag ctc cgc cag tcc acc tcg ctc acg cac gtc ttc aat cgc ttc 15428 Gln Leu Arg Gln Ser Thr Ser Leu Thr His Val Phe Asn Arg Phe 2085 2090 2095 cct gag aat cag atc ctc atc cgc ccg ccg gcg ccc acc att acc 15473 Pro Glu Asn Gln Ile Leu Ile Arg Pro Pro Ala Pro Thr Ile Thr 2100 2105 2110 acc gtc agt gaa aac gtt cct gct ctc aca gat cac ggg acc ctg 15518 Thr Val Ser Glu Asn Val Pro Ala Leu Thr Asp His Gly Thr Leu 2115 2120 2125 ccg ttg cgc agc agt atc cgg gga gtc cag cgc gtg acc gtt act 15563 Pro Leu Arg Ser Ser Ile Arg Gly Val Gln Arg Val Thr Val Thr 2130 2135 2140 gac gcc aga cgc cgc acc tgc ccc tac gtc tac aag gcc ctg ggc 15608 Asp Ala Arg Arg Arg Thr Cys Pro Tyr Val Tyr Lys Ala Leu Gly 2145 2150 2155 ata gtc gcg ccg cgc gtc ctt tca agc cgc act ttc taaaaa atg tcc 15656 Ile Val Ala Pro Arg Val Leu Ser Ser Arg Thr Phe Met Ser 2160 2165 2170 att ctc atc tca ccc agt aat aac acc ggt tgg ggg ctg cgc aca 15701 Ile Leu Ile Ser Pro Ser Asn Asn Thr Gly Trp Gly Leu Arg Thr 2175 2180 2185 ccc acc agg atg tac gga ggc gct cgc aaa cgg tct acc cag cac 15746 Pro Thr Arg Met Tyr Gly Gly Ala Arg Lys Arg Ser Thr Gln His 2190 2195 2200 cct gtg cgt gtg cgc ggg cat ttc cgc gct ccc tgg ggc gcc ctc 15791 Pro Val Arg Val Arg Gly His Phe Arg Ala Pro Trp Gly Ala Leu 2205 2210 2215 aag ggc cgc gct cgc act cgg acc acc gtc gat gat gtg atc gac 15836 Lys Gly Arg Ala Arg Thr Arg Thr Thr Val Asp Asp Val Ile Asp 2220 2225 2230 cag gtg gtt gca gat gct cgt aat tat act cct gct gca cct aca 15881 Gln Val Val Ala Asp Ala Arg Asn Tyr Thr Pro Ala Ala Pro Thr 2235 2240 2245 tct act gtg gat gca gtt att gac agc gtg gtg gct gac gct cgc 15926 Ser Thr Val Asp Ala Val Ile Asp Ser Val Val Ala Asp Ala Arg 2250 2255 2260 gac tat gct cgc cgg aag agc agg cga aga cgc atc gcc aga cgc 15971 Asp Tyr Ala Arg Arg Lys Ser Arg Arg Arg Arg Ile Ala Arg Arg 2265 2270 2275 cac cgg gct acc ccc gcc atg cga gct gca aga gct ctg ctg cgg 16016 His Arg Ala Thr Pro Ala Met Arg Ala Ala Arg Ala Leu Leu Arg 2280 2285 2290 aga gct aaa cgc gtg ggg cga aga gcc atg ctt aga gcg gcc aga 16061 Arg Ala Lys Arg Val Gly Arg Arg Ala Met Leu Arg Ala Ala Arg 2295 2300 2305 cgc gcg gct tca ggt gcc agc gca ggc aga tcc cgc agg cgc gcg 16106 Arg Ala Ala Ser Gly Ala Ser Ala Gly Arg Ser Arg Arg Arg Ala 2310 2315 2320 gcc acg gcg gca gca gca gcc atc gcc aac atg gcc caa ccg cga 16151 Ala Thr Ala Ala Ala Ala Ala Ile Ala Asn Met Ala Gln Pro Arg 2325 2330 2335 aga ggc aat gtg tac tgg gtg cgc gat gcc act acc ggc cag cgc 16196 Arg Gly Asn Val Tyr Trp Val Arg Asp Ala Thr Thr Gly Gln Arg 2340 2345 2350 gtg ccc gtg cgc acc cgt ccc cct cgc act tagaagatac tgagcagtct 16246 Val Pro Val Arg Thr Arg Pro Pro Arg Thr 2355 2360 ccgatgttgt gtcccagcgg cgagg atg tcc aag cgc aaa tac aag gaa gag 16298 Met Ser Lys Arg Lys Tyr Lys Glu Glu 2365 2370 atg ctc cag gtc atc gcg cct gaa atc tac ggt cca ccg gtg aag 16343 Met Leu Gln Val Ile Ala Pro Glu Ile Tyr Gly Pro Pro Val Lys 2375 2380 2385 gat gaa aaa aag ccc cgc aaa atc aag cgg gtc aaa aag gac aaa 16388 Asp Glu Lys Lys Pro Arg Lys Ile Lys Arg Val Lys Lys Asp Lys 2390 2395 2400 aag gaa gaa gat ggc gat gat ggg ctg gtg gag ttt gtg cgc gag 16433 Lys Glu Glu Asp Gly Asp Asp Gly Leu Val Glu Phe Val Arg Glu 2405 2410 2415 ttc gct cca agg cgg cgc gtg cag tgg cgc ggg cgc agg gtg cgg 16478 Phe Ala Pro Arg Arg Arg Val Gln Trp Arg Gly Arg Arg Val Arg 2420 2425 2430 ccg gtg ctg aga ccc gga acc acg gtg gtc ttc acg ccc ggc gaa 16523 Pro Val Leu Arg Pro Gly Thr Thr Val Val Phe Thr Pro Gly Glu 2435 2440 2445 cgc tcc agc act act ttt aaa cgc tcc tat gat gag gtg tac ggg 16568 Arg Ser Ser Thr Thr Phe Lys Arg Ser Tyr Asp Glu Val Tyr Gly 2450 2455 2460 gat gat gat att ctg gag cag gca gcc gac cgc ctg ggc gag ttt 16613 Asp Asp Asp Ile Leu Glu Gln Ala Ala Asp Arg Leu Gly Glu Phe 2465 2470 2475 gct tat ggc aaa cgc agc cgc tcc agt cct aag gag gag gcg gtg 16658 Ala Tyr Gly Lys Arg Ser Arg Ser Ser Pro Lys Glu Glu Ala Val 2480 2485 2490 tcc atc ccc ttg gat cat gga aat ccc acc ccg agt ctt aaa cca 16703 Ser Ile Pro Leu Asp His Gly Asn Pro Thr Pro Ser Leu Lys Pro 2495 2500 2505 gtc acc ctg caa caa gtg ctg cct gtg cct cca cgg aga ggt gtc 16748 Val Thr Leu Gln Gln Val Leu Pro Val Pro Pro Arg Arg Gly Val 2510 2515 2520 aag cga gag ggc gag gat ctg tat ccc acc atg caa ttg atg gtg 16793 Lys Arg Glu Gly Glu Asp Leu Tyr Pro Thr Met Gln Leu Met Val 2525 2530 2535 ccc aag cgc cag aag ctg gag gac gtg ctg gag aaa atg aaa gtg 16838 Pro Lys Arg Gln Lys Leu Glu Asp Val Leu Glu Lys Met Lys Val 2540 2545 2550 gat ccc gat atc cag cct gaa gtt aaa gtc aga ccc atc aag cag 16883 Asp Pro Asp Ile Gln Pro Glu Val Lys Val Arg Pro Ile Lys Gln 2555 2560 2565 gtg gcg ccc ggt ctg gga gta caa acc gtg gac atc aag att ccc 16928 Val Ala Pro Gly Leu Gly Val Gln Thr Val Asp Ile Lys Ile Pro 2570 2575 2580 acc gag tcc atg gaa gtc cag act gaa cct gca aag ccc gca gcc 16973 Thr Glu Ser Met Glu Val Gln Thr Glu Pro Ala Lys Pro Ala Ala 2585 2590 2595 acc tcc att gag gtg cag aca gat cca tgg atg ccc gcg ccc att 17018 Thr Ser Ile Glu Val Gln Thr Asp Pro Trp Met Pro Ala Pro Ile 2600 2605 2610 gca acc acc gcc agt acc gct cga aga ccc cgg cga aag tat ggt 17063 Ala Thr Thr Ala Ser Thr Ala Arg Arg Pro Arg Arg Lys Tyr Gly 2615 2620 2625 cct gcg agt ctg ttg atg ccc aat tat gct ctg cac cca tcc att 17108 Pro Ala Ser Leu Leu Met Pro Asn Tyr Ala Leu His Pro Ser Ile 2630 2635 2640 att cca act cct ggt tac cga ggc act cgc tac tac cgc agc agg 17153 Ile Pro Thr Pro Gly Tyr Arg Gly Thr Arg Tyr Tyr Arg Ser Arg 2645 2650 2655 agc act act tcc cgc cgc cgc aaa aca cct gca agc cgc agt cgc 17198 Ser Thr Thr Ser Arg Arg Arg Lys Thr Pro Ala Ser Arg Ser Arg 2660 2665 2670 cgt cgc cgc cgc cgc acc acc agc aaa ctg act ccc gcc gct ctg 17243 Arg Arg Arg Arg Arg Thr Thr Ser Lys Leu Thr Pro Ala Ala Leu 2675 2680 2685 gtg cgg agg gtg tat cgc gat ggc cgc gca gag ccc ctg atg ctg 17288 Val Arg Arg Val Tyr Arg Asp Gly Arg Ala Glu Pro Leu Met Leu 2690 2695 2700 ccg cgc gct cgc tac cat cca agc atc acc act taatgactgt 17331 Pro Arg Ala Arg Tyr His Pro Ser Ile Thr Thr 2705 2710 tgccgctgcc tccttgcaga t atg gcc ctc act tgc cgc ctt cgc gtc ccc 17382 Met Ala Leu Thr Cys Arg Leu Arg Val Pro 2715 2720 att act ggc tac cga gga aga aac tcg cgc cgt aga agg atg ttg 17427 Ile Thr Gly Tyr Arg Gly Arg Asn Ser Arg Arg Arg Arg Met Leu 2725 2730 2735 ggt agc ggg atg cgt cgc cac agg cgg cgg cgc gcc acc agc agg 17472 Gly Ser Gly Met Arg Arg His Arg Arg Arg Arg Ala Thr Ser Arg 2740 2745 2750 agg ctg ggg ggt ggc ttt ctg acc gct ttg att ccc atc atc gcc 17517 Arg Leu Gly Gly Gly Phe Leu Thr Ala Leu Ile Pro Ile Ile Ala 2755 2760 2765 gcg gcg att ggg gca gta cca ggc ata gct tcc gtg gcg gtt cag 17562 Ala Ala Ile Gly Ala Val Pro Gly Ile Ala Ser Val Ala Val Gln 2770 2775 2780 gcc tcg cag cgc cac tgacattgga aaaaaactta taaataaaat agaatggact 17617 Ala Ser Gln Arg His 2785 ctgacgctcc tggtcctgtg actatgtttt tgtagag atg gaa gac atc aat ttt 17672 Met Glu Asp Ile Asn Phe 2790 tca tcc ctg gct ccg cga cac ggc acg agg ccg tac atg ggc acc 17717 Ser Ser Leu Ala Pro Arg His Gly Thr Arg Pro Tyr Met Gly Thr 2795 2800 2805 tgg agc gac atc ggc acc agc caa ctg aac ggg ggc gcc ttc aat 17762 Trp Ser Asp Ile Gly Thr Ser Gln Leu Asn Gly Gly Ala Phe Asn 2810 2815 2820 tgg agc agt atc tgg agc ggg ctt aaa aat ttt ggc tct gcc ata 17807 Trp Ser Ser Ile Trp Ser Gly Leu Lys Asn Phe Gly Ser Ala Ile 2825 2830 2835 aaa acc tat ggg aac aaa gct tgg aac agc agc aca ggg cag gcg 17852 Lys Thr Tyr Gly Asn Lys Ala Trp Asn Ser Ser Thr Gly Gln Ala 2840 2845 2850 ctg agg aat aag ctt aaa gag cag aac ttc cag cag aag gtg gtc 17897 Leu Arg Asn Lys Leu Lys Glu Gln Asn Phe Gln Gln Lys Val Val 2855 2860 2865 gat ggt atc gcc tct ggc atc aat ggg gtg gtg gat ctg gcc aac 17942 Asp Gly Ile Ala Ser Gly Ile Asn Gly Val Val Asp Leu Ala Asn 2870 2875 2880 cag gcc gtg cag aaa cag ata aac agc cgc ctg gac ccg ccg cct 17987 Gln Ala Val Gln Lys Gln Ile Asn Ser Arg Leu Asp Pro Pro Pro 2885 2890 2895 gca gcc cct ggc gaa atg gaa gtg gag gaa gag ctc cct ccc ctg 18032 Ala Ala Pro Gly Glu Met Glu Val Glu Glu Glu Leu Pro Pro Leu 2900 2905 2910 gaa aag cgg gga gac aag cgc ccg cgt ccc gat atg gag gag acg 18077 Glu Lys Arg Gly Asp Lys Arg Pro Arg Pro Asp Met Glu Glu Thr 2915 2920 2925 ctg gtg acg cgc gga gac gag ccg cct cca tat gag gag gca ata 18122 Leu Val Thr Arg Gly Asp Glu Pro Pro Pro Tyr Glu Glu Ala Ile 2930 2935 2940 aag ctt gga atg ccc act acc aag cct ata gct ccc atg gcc acc 18167 Lys Leu Gly Met Pro Thr Thr Lys Pro Ile Ala Pro Met Ala Thr 2945 2950 2955 ggg gta atg aaa cct tct cag tcg cat cga ccc gcc acc ttg gac 18212 Gly Val Met Lys Pro Ser Gln Ser His Arg Pro Ala Thr Leu Asp 2960 2965 2970 ttg cct cct gcc cct gct gct gca gcg ccc gct cca aag cct gtc 18257 Leu Pro Pro Ala Pro Ala Ala Ala Ala Pro Ala Pro Lys Pro Val 2975 2980 2985 gct acc ccg aag ccc acc acc gta cag ccc gtc gcc gta gcc aga 18302 Ala Thr Pro Lys Pro Thr Thr Val Gln Pro Val Ala Val Ala Arg 2990 2995 3000 ccg cgt ccc ggg ggc act ccg cgc ccg aat gca aac tgg cag agt 18347 Pro Arg Pro Gly Gly Thr Pro Arg Pro Asn Ala Asn Trp Gln Ser 3005 3010 3015 act ctg aac agc atc gtg ggt ctg ggc gtg cag agt gta aag cgc 18392 Thr Leu Asn Ser Ile Val Gly Leu Gly Val Gln Ser Val Lys Arg 3020 3025 3030 cgt cgc tgc tat taattaaata tggagtagcg cttaacttgc ttgtctgtgt 18444 Arg Arg Cys Tyr 3035 gtatgtgtca tcaccacgcc gccgcagcag cagcagcagc agaggagaaa ggaagaggtc 18504 gcgcgccgag gctgagttgc tttcaag atg gcc acc cca tcg atg ttg ccc 18555 Met Ala Thr Pro Ser Met Leu Pro 3040 3045 cag tgg gca tac atg cac atc gcc gga cag gat gct tcg gag tac 18600 Gln Trp Ala Tyr Met His Ile Ala Gly Gln Asp Ala Ser Glu Tyr 3050 3055 3060 ctg agt ccg ggt ctg gtg cag ttc gcc cgt gcc aca gac acc tac 18645 Leu Ser Pro Gly Leu Val Gln Phe Ala Arg Ala Thr Asp Thr Tyr 3065 3070 3075 ttc aat ctg ggg aac aag ttt agg aac ccc acc gtg gcc ccc acc 18690 Phe Asn Leu Gly Asn Lys Phe Arg Asn Pro Thr Val Ala Pro Thr 3080 3085 3090 cac gat gtg acc acc gac cga agc cag cgg ctg atg ctg cgc ttt 18735 His Asp Val Thr Thr Asp Arg Ser Gln Arg Leu Met Leu Arg Phe 3095 3100 3105 gtg ccc gtt gat cgg gag gac aat acc tac tca tac aaa gtt cgc 18780 Val Pro Val Asp Arg Glu Asp Asn Thr Tyr Ser Tyr Lys Val Arg 3110 3115 3120 tac aca ctg gct gtg ggc gac aac aga gtg ctg gat atg gcc agc 18825 Tyr Thr Leu Ala Val Gly Asp Asn Arg Val Leu Asp Met Ala Ser 3125 3130 3135 acc ttc ttt gac atc cgg ggg gtg ctt gac aga ggt ccc agt ttc 18870 Thr Phe Phe Asp Ile Arg Gly Val Leu Asp Arg Gly Pro Ser Phe 3140 3145 3150 aag cca tac tct ggc aca gct tac aac tca cta gct cct aaa ggc 18915 Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser Leu Ala Pro Lys Gly 3155 3160 3165 gct ccc aac act tgt cag tgg aag tac aaa aca ctt gct aac aca 18960 Ala Pro Asn Thr Cys Gln Trp Lys Tyr Lys Thr Leu Ala Asn Thr 3170 3175 3180 gaa aca gaa gaa gag gag gag gag gac gaa cag gca gat gaa caa 19005 Glu Thr Glu Glu Glu Glu Glu Glu Asp Glu Gln Ala Asp Glu Gln 3185 3190 3195 gaa tac gtt gaa aag act tct aca ttt gga aat gcg cct gta aaa 19050 Glu Tyr Val Glu Lys Thr Ser Thr Phe Gly Asn Ala Pro Val Lys 3200 3205 3210 gga ctg gat ata gat gca gat ggc ttg cag ata ggt gtg gac ata 19095 Gly Leu Asp Ile Asp Ala Asp Gly Leu Gln Ile Gly Val Asp Ile 3215 3220 3225 caa gat gaa act aaa cca gtc tac gct aac aag ctt tat gaa cca 19140 Gln Asp Glu Thr Lys Pro Val Tyr Ala Asn Lys Leu Tyr Glu Pro 3230 3235 3240 gaa ccc caa gtg gga gat gga caa tgg cat gat acc act gct att 19185 Glu Pro Gln Val Gly Asp Gly Gln Trp His Asp Thr Thr Ala Ile 3245 3250 3255 act gag cag tat gga ggc cgt gct ctt aag cct gac aca aaa atg 19230 Thr Glu Gln Tyr Gly Gly Arg Ala Leu Lys Pro Asp Thr Lys Met 3260 3265 3270 aaa cct tgc tat gga tcg ttt gct aga cct aca aat gaa aaa gga 19275 Lys Pro Cys Tyr Gly Ser Phe Ala Arg Pro Thr Asn Glu Lys Gly 3275 3280 3285 gga caa tct aaa act aga tca gta acg aag gat gac aaa gta gtg 19320 Gly Gln Ser Lys Thr Arg Ser Val Thr Lys Asp Asp Lys Val Val 3290 3295 3300 gaa gag cca gat att gat atg gct ttc ttt gat gga aga gat gcc 19365 Glu Glu Pro Asp Ile Asp Met Ala Phe Phe Asp Gly Arg Asp Ala 3305 3310 3315 aaa aaa cca gat cca gaa att gtt ctg tat act gaa aat gtt aac 19410 Lys Lys Pro Asp Pro Glu Ile Val Leu Tyr Thr Glu Asn Val Asn 3320 3325 3330 ttg gaa aca cct gac act cat atc gtt tac aaa gcc ggc aca gat 19455 Leu Glu Thr Pro Asp Thr His Ile Val Tyr Lys Ala Gly Thr Asp 3335 3340 3345 gat tcc agc tca tct att aat ttg ggc caa cag tct atg ccc aat 19500 Asp Ser Ser Ser Ser Ile Asn Leu Gly Gln Gln Ser Met Pro Asn 3350 3355 3360 aga ccc aac tat att ggc ttc aga gac aac ttc att ggg ctc atg 19545 Arg Pro Asn Tyr Ile Gly Phe Arg Asp Asn Phe Ile Gly Leu Met 3365 3370 3375 tac tat aac agt act ggc aat atg gga gtg ttg gcc gga caa gca 19590 Tyr Tyr Asn Ser Thr Gly Asn Met Gly Val Leu Ala Gly Gln Ala 3380 3385 3390 tcc cag cta aat gca gtg gtt gac ttg cag gac aga aac aca gag 19635 Ser Gln Leu Asn Ala Val Val Asp Leu Gln Asp Arg Asn Thr Glu 3395 3400 3405 ctg tct tac cag ctt ttg ctt gat tct ttg gga gac agg act aga 19680 Leu Ser Tyr Gln Leu Leu Leu Asp Ser Leu Gly Asp Arg Thr Arg 3410 3415 3420 tac ttt agc atg tgg aat cag gca gtg gac agc tat gac cct gat 19725 Tyr Phe Ser Met Trp Asn Gln Ala Val Asp Ser Tyr Asp Pro Asp 3425 3430 3435 gtc cgt att att gaa aac cat ggt gtg gaa gac gaa ctt ccc aat 19770 Val Arg Ile Ile Glu Asn His Gly Val Glu Asp Glu Leu Pro Asn 3440 3445 3450 tac tgt ttc cca ttg gat gga gtg ggt cgc aca gat act tac aaa 19815 Tyr Cys Phe Pro Leu Asp Gly Val Gly Arg Thr Asp Thr Tyr Lys 3455 3460 3465 ggc gtc gta gta gat caa gct gca gca gca gga act gca act act 19860 Gly Val Val Val Asp Gln Ala Ala Ala Ala Gly Thr Ala Thr Thr 3470 3475 3480 tgg aaa gat gat acc act gca aat gaa tac aat gaa att gct aag 19905 Trp Lys Asp Asp Thr Thr Ala Asn Glu Tyr Asn Glu Ile Ala Lys 3485 3490 3495 ggt aat aat cta gcc atg gaa att aat ctc caa gct aac tta tgg 19950 Gly Asn Asn Leu Ala Met Glu Ile Asn Leu Gln Ala Asn Leu Trp 3500 3505 3510 aga agt ttt ctt tac tcc aat gta gct ttg tac ctt ccg gat gct 19995 Arg Ser Phe Leu Tyr Ser Asn Val Ala Leu Tyr Leu Pro Asp Ala 3515 3520 3525 tac aaa tac act ccg gct aat gtc act ctc cct act aac act aat 20040 Tyr Lys Tyr Thr Pro Ala Asn Val Thr Leu Pro Thr Asn Thr Asn 3530 3535 3540 acc tat gaa tac atg aat ggg agg gta gtg tcg cca tct ttg gtg 20085 Thr Tyr Glu Tyr Met Asn Gly Arg Val Val Ser Pro Ser Leu Val 3545 3550 3555 gac gct tat gta aac att ggc gca aga tgg tct ttg gat cct atg 20130 Asp Ala Tyr Val Asn Ile Gly Ala Arg Trp Ser Leu Asp Pro Met 3560 3565 3570 gac aat gtt aac ccc ttt aat cat cac cgc aat gct ggc ctg cgc 20175 Asp Asn Val Asn Pro Phe Asn His His Arg Asn Ala Gly Leu Arg 3575 3580 3585 tat cgg tca atg ctt ctg ggc aac ggt cgc tat gtg ccc ttc cac 20220 Tyr Arg Ser Met Leu Leu Gly Asn Gly Arg Tyr Val Pro Phe His 3590 3595 3600 atc caa gtg cct cag aaa ttt ttt gct gtg aaa aac ctg ctt ctc 20265 Ile Gln Val Pro Gln Lys Phe Phe Ala Val Lys Asn Leu Leu Leu 3605 3610 3615 ctc cca ggc tcc tac acc tat gag tgg aac ttt cgc aag gat gta 20310 Leu Pro Gly Ser Tyr Thr Tyr Glu Trp Asn Phe Arg Lys Asp Val 3620 3625 3630 aat atg gtc ttg caa agt tct ctt ggc aac gac ctc aga aca gat 20355 Asn Met Val Leu Gln Ser Ser Leu Gly Asn Asp Leu Arg Thr Asp 3635 3640 3645 ggt gct acc atc agt ttt acc agc att aat ctc tat gcc acc ttc 20400 Gly Ala Thr Ile Ser Phe Thr Ser Ile Asn Leu Tyr Ala Thr Phe 3650 3655 3660 ttc ccc atg gct cac aac act gct tcc act ctt gaa gcc atg ctg 20445 Phe Pro Met Ala His Asn Thr Ala Ser Thr Leu Glu Ala Met Leu 3665 3670 3675 cgc aat gac acc aat gac cag tca ttc aat gac tac ctc tct gca 20490 Arg Asn Asp Thr Asn Asp Gln Ser Phe Asn Asp Tyr Leu Ser Ala 3680 3685 3690 gct aac atg ctc tac cca att cca gca aat gcc acc aac att ccc 20535 Ala Asn Met Leu Tyr Pro Ile Pro Ala Asn Ala Thr Asn Ile Pro 3695 3700 3705 att tcc att ccc tct cgc aac tgg gct gcc ttc agg ggc tgg tcc 20580 Ile Ser Ile Pro Ser Arg Asn Trp Ala Ala Phe Arg Gly Trp Ser 3710 3715 3720 ttc acc aga ctc aaa act aag gaa act ccc tct ttg gga tca ggc 20625 Phe Thr Arg Leu Lys Thr Lys Glu Thr Pro Ser Leu Gly Ser Gly 3725 3730 3735 ttt gat ccc tac ttt gtt tat tct ggc tct att ccc tac ctg gat 20670 Phe Asp Pro Tyr Phe Val Tyr Ser Gly Ser Ile Pro Tyr Leu Asp 3740 3745 3750 ggt acc ttc tac ctc aac cac act ttc aag aag gtg tcc atc atg 20715 Gly Thr Phe Tyr Leu Asn His Thr Phe Lys Lys Val Ser Ile Met 3755 3760 3765 ttt gac tcc tca gtt agc tgg cct ggc aat gac aga ttg cta act 20760 Phe Asp Ser Ser Val Ser Trp Pro Gly Asn Asp Arg Leu Leu Thr 3770 3775 3780 cca aat gag ttt gaa atc aag cgc act gtg gat gga gaa ggg tac 20805 Pro Asn Glu Phe Glu Ile Lys Arg Thr Val Asp Gly Glu Gly Tyr 3785 3790 3795 aat gtg gct caa tgc aac atg acc aag gac tgg ttc ctg gtt cag 20850 Asn Val Ala Gln Cys Asn Met Thr Lys Asp Trp Phe Leu Val Gln 3800 3805 3810 atg ctt gcc aac tac aac att ggc tac cag ggc ttc tac atc cca 20895 Met Leu Ala Asn Tyr Asn Ile Gly Tyr Gln Gly Phe Tyr Ile Pro 3815 3820 3825 gag ggg tac aag gat cgc atg tac tcc ttc ttc aga aac ttc cag 20940 Glu Gly Tyr Lys Asp Arg Met Tyr Ser Phe Phe Arg Asn Phe Gln 3830 3835 3840 ccc atg agt agg cag gtg gtt gat gag atc aac tac act gac tac 20985 Pro Met Ser Arg Gln Val Val Asp Glu Ile Asn Tyr Thr Asp Tyr 3845 3850 3855 aag gct gtt aag ctt cca ttc caa cac aac aac tct gga ttt gtg 21030 Lys Ala Val Lys Leu Pro Phe Gln His Asn Asn Ser Gly Phe Val 3860 3865 3870 ggt tac ctc gct cca acc att cgt cag ggt caa gct tat cca gct 21075 Gly Tyr Leu Ala Pro Thr Ile Arg Gln Gly Gln Ala Tyr Pro Ala 3875 3880 3885 aac tac cca tac ccc cta att gga tcc act gct gtt aaa agc gtt 21120 Asn Tyr Pro Tyr Pro Leu Ile Gly Ser Thr Ala Val Lys Ser Val 3890 3895 3900 acc cag aaa aag ttc ttg tgc gac agg acc atg tgg cgc atc cca 21165 Thr Gln Lys Lys Phe Leu Cys Asp Arg Thr Met Trp Arg Ile Pro 3905 3910 3915 ttc tcc agc aac ttc atg tcc atg ggt gcc cta acc gac ctg ggg 21210 Phe Ser Ser Asn Phe Met Ser Met Gly Ala Leu Thr Asp Leu Gly 3920 3925 3930 cag aac atg ctt tat gcc aac tca gcc cat gcg ctg gac atg act 21255 Gln Asn Met Leu Tyr Ala Asn Ser Ala His Ala Leu Asp Met Thr 3935 3940 3945 ttt gag gtg gat ccc atg gat gag ccc aca ctg ctt tat ctt ctt 21300 Phe Glu Val Asp Pro Met Asp Glu Pro Thr Leu Leu Tyr Leu Leu 3950 3955 3960 ttt gaa gtc ttc gac gtg gtc aga gtg cac cag cca cac cgc ggc 21345 Phe Glu Val Phe Asp Val Val Arg Val His Gln Pro His Arg Gly 3965 3970 3975 gtc atc gag gct gtc tac ctg cgt acc cca ttc tca gct ggt aac 21390 Val Ile Glu Ala Val Tyr Leu Arg Thr Pro Phe Ser Ala Gly Asn 3980 3985 3990 gcc acc aca taagcctctt gcttcttgca agcagctgcc atg gcc tgt ggg tct 21444 Ala Thr Thr Met Ala Cys Gly Ser 3995 ggc aac gga tcc agc gag caa gag ctc agg gcc att gct aga gac 21489 Gly Asn Gly Ser Ser Glu Gln Glu Leu Arg Ala Ile Ala Arg Asp 4000 4005 4010 ctg gga tgc gga ccc tat ttc ctg gga acc ttt gac aag cgt ttc 21534 Leu Gly Cys Gly Pro Tyr Phe Leu Gly Thr Phe Asp Lys Arg Phe 4015 4020 4025 ccg ggg ttc atg gcc ccc gac aag ctc gcc tgc gcc att gtt aac 21579 Pro Gly Phe Met Ala Pro Asp Lys Leu Ala Cys Ala Ile Val Asn 4030 4035 4040 acg gct ggt cgc gag acg ggg gga gag cac tgg ctg gct ttt ggt 21624 Thr Ala Gly Arg Glu Thr Gly Gly Glu His Trp Leu Ala Phe Gly 4045 4050 4055 tgg aac ccg cgc tcc aac acc tgc tac ctt ttt gat cct ttt ggc 21669 Trp Asn Pro Arg Ser Asn Thr Cys Tyr Leu Phe Asp Pro Phe Gly 4060 4065 4070 ttc tcg gat gag cgc ctc aag caa atc tac cag ttt gag tat gag 21714 Phe Ser Asp Glu Arg Leu Lys Gln Ile Tyr Gln Phe Glu Tyr Glu 4075 4080 4085 ggt ctc ctg cgc cgc agt gcc ctg gct acc aag gat cgc tgt atc 21759 Gly Leu Leu Arg Arg Ser Ala Leu Ala Thr Lys Asp Arg Cys Ile 4090 4095 4100 acc ctg gaa aag tcc acc cag acc gtg cag ggc ccg cgc tcc gca 21804 Thr Leu Glu Lys Ser Thr Gln Thr Val Gln Gly Pro Arg Ser Ala 4105 4110 4115 gcc tgt gga ctt ttt tgc tgc atg ttc ctc cac gct ttt gtg cac 21849 Ala Cys Gly Leu Phe Cys Cys Met Phe Leu His Ala Phe Val His 4120 4125 4130 tgg ccc gac cgc ccc atg gac gga aac ccc acc atg aag ttg ttg 21894 Trp Pro Asp Arg Pro Met Asp Gly Asn Pro Thr Met Lys Leu Leu 4135 4140 4145 act ggg gtg ccc aac agc atg ctc caa tca ccc caa gtc cag ccc 21939 Thr Gly Val Pro Asn Ser Met Leu Gln Ser Pro Gln Val Gln Pro 4150 4155 4160 acc ctg cgc cac aac cag gag gcg ctc tac cgc ttc ctt aac acc 21984 Thr Leu Arg His Asn Gln Glu Ala Leu Tyr Arg Phe Leu Asn Thr 4165 4170 4175 cac tca tct tac ttt cgt tct cac cgc gcg cgc atc gaa aag gcc 22029 His Ser Ser Tyr Phe Arg Ser His Arg Ala Arg Ile Glu Lys Ala 4180 4185 4190 acc gcg ttt gac cgt atg gct atg caa taataagtca tgtaaaaacc 22076 Thr Ala Phe Asp Arg Met Ala Met Gln 4195 4200 gtgtttcaaa taaacagcac tttatttttt acatgcactg tggctctggg ttgctcattc 22136 attcatcatt cactcagaag tcgaaggggt tctggcggga atcagcgtga cccgctggca 22196 gggatacgtt gcggaactgg aacctgttct gccacttgaa ctcggggatc accagtttgg 22256 gaactaggat ctcggggaag gtgtcttgcc acagctttct ggtcagttgc agagcgccga 22316 gcaggtcagg agcagagatc ttgaaatcac agttggggcc agcattctga gcgcgggagt 22376 tgcggtacac tgggttacag cactggaaca ccatcagggc ggggtgtctc acgctcgcca 22436 gcacggtcgg gtcactgatg gtagacacat ccaagtcttc agcattggcc attccaaagg 22496 gggtcatctt acaggtctgc ctgcccatca cgggagcgca gccgggcttg tggttgcaat 22556 cgcagtgaat ggggatcagc atcatcctgg cctggtcggg ggttatccct ggataaaccg 22616 ccttcataaa ggcttcgtac tgcttgaaag cttcctgggc cttgcttccc tcggtgtaga 22676 acatcccaca tgacttgctg gaaaactgat tagtagcaca gttggcatca ttcacacagc 22736 agcgggcatc gttgttggcc agctggacca cattcctgcc ccagcggttc tgggtgatct 22796 tggctcggtc tgggttctcc ttcatcgcgc gctgcccgtt ctcgctcgcc acatccatct 22856 cgatgatgtg atccttctgg atcatgatag tgccatgcag gcatttcacc ttgccttcat 22916 aatcggtgca gccatgagcc cacagagcgc acccggtgca ctcccaattg ttgtgggcga 22976 tctcagaata agaatgcacc aacccctgca ggaatcttcc catcatggtt gagagggtct 23036 tgttactggt gaaagtcagc gggacgcctc gatgctcctc gttcacatac tggtggcaaa 23096 ttcgcttgta ctgttcatgc tgctctggca taagcttgaa agaggttctt aggtcattct 23156 ccagcctgta cttctccatc agcacagcca ttacttccat gcccttttcc caggcagaaa 23216 ccaggggtag gctcatggaa tttctaacag aaatagcagc tactttagcc agagggtcat 23276 ccttgtcaat cttctcaaca cttcttttgc catccttctc agtgatgcgc acgggtgggt 23336 agctgaagcc cacggccacc agctccgcct cttctctttc ttcttcgctg tcctgactga 23396 tgtcttgtaa agggacatgc ttggtcttcc tgggcttctt tttggggggt attggcggag 23456 ggctgctgct ccgctccgga gacatggagg accgcgaagt ttcgctcacc agtaccacct 23516 ggctctcggt agaagaaccg gaccccacac ggcggtaggt gttcctcttc gggggcagag 23576 gtggaggtga ctgcgatggg ctgcggtccg gcctgggagg cggatgactg gcagagcccc 23636 ttccgcgttc gggggtgtgc tcccggtggc ggtcgcttga ctgatttcct ccgcggctgg 23696 ccattgtgtt ctcctaggca gagaaacaac agac atg gag act cag cca tcg 23748 Met Glu Thr Gln Pro Ser 4205 ctg cca aca ccg ctg caa gca cca tca cac ctc gcc tcc agc gat 23793 Leu Pro Thr Pro Leu Gln Ala Pro Ser His Leu Ala Ser Ser Asp 4210 4215 4220 gag gag gag gaa caa agc tta acc gcc cca cca ccc agt ccc gcc 23838 Glu Glu Glu Glu Gln Ser Leu Thr Ala Pro Pro Pro Ser Pro Ala 4225 4230 4235 acc acc acc tct acc ctc gag gat gag gag gtc gac gca ccc cag 23883 Thr Thr Thr Ser Thr Leu Glu Asp Glu Glu Val Asp Ala Pro Gln 4240 4245 4250 gag ata cgg acg cag gat atg gag gat gag aaa gcg gaa gag att 23928 Glu Ile Arg Thr Gln Asp Met Glu Asp Glu Lys Ala Glu Glu Ile 4255 4260 4265 gag gca gat atc gag cag gac cca ggc tat gtg aca ccg gcc gag 23973 Glu Ala Asp Ile Glu Gln Asp Pro Gly Tyr Val Thr Pro Ala Glu 4270 4275 4280 cac gag gaa gag ctg aga cgc ttt cta gag aaa gat gat gac aac 24018 His Glu Glu Glu Leu Arg Arg Phe Leu Glu Lys Asp Asp Asp Asn 4285 4290 4295 cgt cca gaa cag caa gca gat ggc gat cag cag aat gtt ggg ctc 24063 Arg Pro Glu Gln Gln Ala Asp Gly Asp Gln Gln Asn Val Gly Leu 4300 4305 4310 ggg gat cat gtt gtc gac tac ctc acc ggc ctt ggt ggg gag gac 24108 Gly Asp His Val Val Asp Tyr Leu Thr Gly Leu Gly Gly Glu Asp 4315 4320 4325 gtg ctc ctc aaa cac cta gca agg cag tcg atc ata atc aaa gat 24153 Val Leu Leu Lys His Leu Ala Arg Gln Ser Ile Ile Ile Lys Asp 4330 4335 4340 gca ctg ctt gat cgc agc gaa gtg ccc atc agt gtg gaa gag ctc 24198 Ala Leu Leu Asp Arg Ser Glu Val Pro Ile Ser Val Glu Glu Leu 4345 4350 4355 agc cgc gcc tac gag ctc aac ctg ttc tcg cct cgg gta ccc ccc 24243 Ser Arg Ala Tyr Glu Leu Asn Leu Phe Ser Pro Arg Val Pro Pro 4360 4365 4370 aag cgt cag cca aac ggc acc tgc gag ccc aac cct cgc ctc aac 24288 Lys Arg Gln Pro Asn Gly Thr Cys Glu Pro Asn Pro Arg Leu Asn 4375 4380 4385 ttc tat ccc gca ttc acc gtc ccc gag gtg ctg gct acc tac cac 24333 Phe Tyr Pro Ala Phe Thr Val Pro Glu Val Leu Ala Thr Tyr His 4390 4395 4400 ata ttt ttc aaa aac caa aaa att cca att tcc tgc cgc gcc aac 24378 Ile Phe Phe Lys Asn Gln Lys Ile Pro Ile Ser Cys Arg Ala Asn 4405 4410 4415 cga act cgc gcc gat gcc ctg ctc aac ttg gga cct ggc gct tgc 24423 Arg Thr Arg Ala Asp Ala Leu Leu Asn Leu Gly Pro Gly Ala Cys 4420 4425 4430 tta cct gat ata act tcc ttg gaa gag gtc cca aag atc ttc gaa 24468 Leu Pro Asp Ile Thr Ser Leu Glu Glu Val Pro Lys Ile Phe Glu 4435 4440 4445 ggt ctg ggc agt gat gag act cgg gcc gca aat gct ctg caa cag 24513 Gly Leu Gly Ser Asp Glu Thr Arg Ala Ala Asn Ala Leu Gln Gln 4450 4455 4460 gga gag aat ggc atc gat gaa cat cac agc gct ctg gtg gag ttg 24558 Gly Glu Asn Gly Ile Asp Glu His His Ser Ala Leu Val Glu Leu 4465 4470 4475 gag ggc gat aat gcc cga cta gca gta ctc aag cgc agt atc gag 24603 Glu Gly Asp Asn Ala Arg Leu Ala Val Leu Lys Arg Ser Ile Glu 4480 4485 4490 gtg acc cat ttt gca tac ccc gct gtc aac ctg cct ccc aaa gtc 24648 Val Thr His Phe Ala Tyr Pro Ala Val Asn Leu Pro Pro Lys Val 4495 4500 4505 atg agc gct gtc atg gat cag ata ctc att aaa cgc gca agt ccc 24693 Met Ser Ala Val Met Asp Gln Ile Leu Ile Lys Arg Ala Ser Pro 4510 4515 4520 ctt tca gaa aac atg cag gat cca gac gcc tcg gat gag ggc aag 24738 Leu Ser Glu Asn Met Gln Asp Pro Asp Ala Ser Asp Glu Gly Lys 4525 4530 4535 cca gtg gtc agt gat gaa cag cta tct cgc tgg ctg ggc acc aac 24783 Pro Val Val Ser Asp Glu Gln Leu Ser Arg Trp Leu Gly Thr Asn 4540 4545 4550 tcc cca cga gac ttg gaa gag cgg cgc aag ctc atg atg gcc gtg 24828 Ser Pro Arg Asp Leu Glu Glu Arg Arg Lys Leu Met Met Ala Val 4555 4560 4565 gtg cta gtt act gtg gaa atg gag tgt ctt cgc cgc ttc ttc act 24873 Val Leu Val Thr Val Glu Met Glu Cys Leu Arg Arg Phe Phe Thr 4570 4575 4580 gac ccc gag aca ctg cgc aag ctc gag gag aac cta cac tac act 24918 Asp Pro Glu Thr Leu Arg Lys Leu Glu Glu Asn Leu His Tyr Thr 4585 4590 4595 ttt aga cat gga ttt gtg aga cag gca tgc aag atc tcc aac gtg 24963 Phe Arg His Gly Phe Val Arg Gln Ala Cys Lys Ile Ser Asn Val 4600 4605 4610 gag ctt acc aac ctg gtt tcc tac atg ggc att ttg cat gaa aac 25008 Glu Leu Thr Asn Leu Val Ser Tyr Met Gly Ile Leu His Glu Asn 4615 4620 4625 aga ctc gga cag agc gtg ctg cac acc acc ctg aag ggg gaa gcc 25053 Arg Leu Gly Gln Ser Val Leu His Thr Thr Leu Lys Gly Glu Ala 4630 4635 4640 cgt cgc gac tac atc cgc gac act gtc tac ctc tac ctc tgc cat 25098 Arg Arg Asp Tyr Ile Arg Asp Thr Val Tyr Leu Tyr Leu Cys His 4645 4650 4655 acc tgg cag act ggt atg ggt gtg tgg cag cag tgt ttg gaa gaa 25143 Thr Trp Gln Thr Gly Met Gly Val Trp Gln Gln Cys Leu Glu Glu 4660 4665 4670 caa aac ctg aaa gaa cta gac aag ctc tta cag aga tcc ctc aaa 25188 Gln Asn Leu Lys Glu Leu Asp Lys Leu Leu Gln Arg Ser Leu Lys 4675 4680 4685 acc ttg tgg acg ggt ttt gac gag cgc aca gtc gcc tct gat ctg 25233 Thr Leu Trp Thr Gly Phe Asp Glu Arg Thr Val Ala Ser Asp Leu 4690 4695 4700 gca gat ctc atc ttc cca gag cgt ctc agg act act ctg cgc aac 25278 Ala Asp Leu Ile Phe Pro Glu Arg Leu Arg Thr Thr Leu Arg Asn 4705 4710 4715 ggg ctg cct gac ttc atg aac cag agc atg att aac aac ttt cgc 25323 Gly Leu Pro Asp Phe Met Asn Gln Ser Met Ile Asn Asn Phe Arg 4720 4725 4730 tct ttc atc ctg gaa cgc tcc ggt atc ctg ccc gcc acc tgc tgt 25368 Ser Phe Ile Leu Glu Arg Ser Gly Ile Leu Pro Ala Thr Cys Cys 4735 4740 4745 gcg cta cca tcc gac ttt gtg cct ctg acc tac cgc gag tgc ccc 25413 Ala Leu Pro Ser Asp Phe Val Pro Leu Thr Tyr Arg Glu Cys Pro 4750 4755 4760 cca ccg cta tgg agc cac tgc tac ctg ttc cgc ctg gcc aac tac 25458 Pro Pro Leu Trp Ser His Cys Tyr Leu Phe Arg Leu Ala Asn Tyr 4765 4770 4775 cta tca tac cac tcg gat gtg atc gag gat gtg agc gga gat ggc 25503 Leu Ser Tyr His Ser Asp Val Ile Glu Asp Val Ser Gly Asp Gly 4780 4785 4790 ctg ctt gag tgc cac tgc cgc tgt aat ctc tgc tca cca cat cgc 25548 Leu Leu Glu Cys His Cys Arg Cys Asn Leu Cys Ser Pro His Arg 4795 4800 4805 tcc ctc gtc tgt aac ccc cag ttg ctt agc gaa acc caa att ata 25593 Ser Leu Val Cys Asn Pro Gln Leu Leu Ser Glu Thr Gln Ile Ile 4810 4815 4820 ggc acc ttc gaa ttg cag ggt ccc agc agc gaa ggc gag ggg tct 25638 Gly Thr Phe Glu Leu Gln Gly Pro Ser Ser Glu Gly Glu Gly Ser 4825 4830 4835 tct cct ggg caa agt ttg aaa ctg acc ccg gga ctg tgg acc tcc 25683 Ser Pro Gly Gln Ser Leu Lys Leu Thr Pro Gly Leu Trp Thr Ser 4840 4845 4850 gcc tac ctg cgc aag ttc tcc ccc gag gac tac cac ccc tat gag 25728 Ala Tyr Leu Arg Lys Phe Ser Pro Glu Asp Tyr His Pro Tyr Glu 4855 4860 4865 atc agg ttc tat gaa gac caa tca cag ccg ccc aaa gct gag ctc 25773 Ile Arg Phe Tyr Glu Asp Gln Ser Gln Pro Pro Lys Ala Glu Leu 4870 4875 4880 tca gcg tgc gtc atc acc cag ggg gca att ttg gcc caa ttg caa 25818 Ser Ala Cys Val Ile Thr Gln Gly Ala Ile Leu Ala Gln Leu Gln 4885 4890 4895 gcc atc caa aaa tcc cgc caa gaa ttt ttg ctg aaa aag ggt aac 25863 Ala Ile Gln Lys Ser Arg Gln Glu Phe Leu Leu Lys Lys Gly Asn 4900 4905 4910 gga gtc tac ctc gac ccc cag act ggt gag gag ctc aac aca agg 25908 Gly Val Tyr Leu Asp Pro Gln Thr Gly Glu Glu Leu Asn Thr Arg 4915 4920 4925 ttc tct cag gat gtc tca gcg ccg agg aaa caa gaa gtt gaa agt 25953 Phe Ser Gln Asp Val Ser Ala Pro Arg Lys Gln Glu Val Glu Ser 4930 4935 4940 gca gct gcc gcc ccc aga gga tat gga gga aga ctg gga cag tca 25998 Ala Ala Ala Ala Pro Arg Gly Tyr Gly Gly Arg Leu Gly Gln Ser 4945 4950 4955 gac aga gga gat gga aga ttg gga cag cca ggc aga gga gga gga 26043 Asp Arg Gly Asp Gly Arg Leu Gly Gln Pro Gly Arg Gly Gly Gly 4960 4965 4970 gga cag cct gga gga aga cag ttt gga gga gga aga cga gga ggc 26088 Gly Gln Pro Gly Gly Arg Gln Phe Gly Gly Gly Arg Arg Gly Gly 4975 4980 4985 aga gga ggt gga aga agc aac cgc cgc caa aca gtt gtc ctc ggc 26133 Arg Gly Gly Gly Arg Ser Asn Arg Arg Gln Thr Val Val Leu Gly 4990 4995 5000 ggc gga gac aag caa ggc cac aga caa cac cac agc tac cat ctc 26178 Gly Gly Asp Lys Gln Gly His Arg Gln His His Ser Tyr His Leu 5005 5010 5015 cgt tcc ggg tcg ggg ggt cca gca ccg tcc caa cag tagatgggat 26224 Arg Ser Gly Ser Gly Gly Pro Ala Pro Ser Gln Gln 5020 5025 5030 gagaccgggc gactcccgaa tgcgaccacc gcttctaaga ctggtaagaa ggagcggcag 26284 ggatacaagt cctggcgggg gcataagaac gctatcatat cctgcttgca tgaatgcggg 26344 ggcaacatat ccttcacccg ccgctacctg ctcttccacc acggggtgaa cttcccccgc 26404 aatgtcttgc attactaccg tcacctccac agcccctact acagccagca agcctcggca 26464 gaaaaagaca acagcagcaa gaacctccag cagaaaacca gcagcagtta gaacacccac 26524 agcaggtgca acaggaggag gactgagaat cacagcgaac gagccagcgc agacccgaga 26584 gctgagaaac cggatttttc caaccctcta tgccatcttc caacagagtc gggggcaaga 26644 gcaggaactg aaagtaaaaa accgatcttt gcgctcgctc acccgaagtt gtttgtatca 26704 caagagcgaa gaccaacttc agcgcactct cgaggacgcc gaggctctct tcaacaagta 26764 ctgcgcgctc actcttaaag agtagcccgc gcccgcgcta gctcgaaaaa aggcgggaat 26824 tacgtcaccc attggcgcct gtcctttgcc ctcgtc atg agt aaa gaa att ccc 26878 Met Ser Lys Glu Ile Pro 5035 acg cct tac atg tgg agt tat caa ccc caa atg gga ctg gca gca 26923 Thr Pro Tyr Met Trp Ser Tyr Gln Pro Gln Met Gly Leu Ala Ala 5040 5045 5050 ggc gcc tcc cag gac tac tcc acc cgt atg aat tgg ctc agc gcc 26968 Gly Ala Ser Gln Asp Tyr Ser Thr Arg Met Asn Trp Leu Ser Ala 5055 5060 5065 ggt ccc tcg atg atc tca cgg gtt aat gat ata cga gct tat cga 27013 Gly Pro Ser Met Ile Ser Arg Val Asn Asp Ile Arg Ala Tyr Arg 5070 5075 5080 aac caa tta ctc cta gaa cag tca gca ctt acc gcc aca ccc aga 27058 Asn Gln Leu Leu Leu Glu Gln Ser Ala Leu Thr Ala Thr Pro Arg 5085 5090 5095 caa cac ctt aat ccc cgg aat tgg ccc gcc gcc ctg gtg tac cag 27103 Gln His Leu Asn Pro Arg Asn Trp Pro Ala Ala Leu Val Tyr Gln 5100 5105 5110 gaa acc ccc gct ccc acc acc gtc cta ctt cct cga gac gcc cag 27148 Glu Thr Pro Ala Pro Thr Thr Val Leu Leu Pro Arg Asp Ala Gln 5115 5120 5125 gcc gaa gtt cag atg act aac gca ggt gta cag ctg gct ggc ggt 27193 Ala Glu Val Gln Met Thr Asn Ala Gly Val Gln Leu Ala Gly Gly 5130 5135 5140 tcc gcc ctg tgt cgt cac cgg cct caa cag agt ata aaa cgc ctg 27238 Ser Ala Leu Cys Arg His Arg Pro Gln Gln Ser Ile Lys Arg Leu 5145 5150 5155 gtg atc aga ggc cga ggt atc cag ctc aac gac gag tcg gtg agc 27283 Val Ile Arg Gly Arg Gly Ile Gln Leu Asn Asp Glu Ser Val Ser 5160 5165 5170 tct tcg ctt ggt cta cga cca gac gga gtc ttc caa att gcc ggc 27328 Ser Ser Leu Gly Leu Arg Pro Asp Gly Val Phe Gln Ile Ala Gly 5175 5180 5185 tgc ggg aga tct tcc ttc act cct cgt cag gct gta ctg act ttg 27373 Cys Gly Arg Ser Ser Phe Thr Pro Arg Gln Ala Val Leu Thr Leu 5190 5195 5200 gag agt tcg tca tcg cag ccc cgc tcg ggt ggc atc ggg act ctc 27418 Glu Ser Ser Ser Ser Gln Pro Arg Ser Gly Gly Ile Gly Thr Leu 5205 5210 5215 caa ttt gtg gag gag ttt act ccc tct gtc tac ttc aac ccc ttc 27463 Gln Phe Val Glu Glu Phe Thr Pro Ser Val Tyr Phe Asn Pro Phe 5220 5225 5230 tcc ggc tct cct ggg cat tat ccg gac gag ttc ata cca aac ttc 27508 Ser Gly Ser Pro Gly His Tyr Pro Asp Glu Phe Ile Pro Asn Phe 5235 5240 5245 gac gca atc agc gag tca gtg gat ggc tat gat tg atg tct aat ggt 27555 Asp Ala Ile Ser Glu Ser Val Asp Gly Tyr Asp Met Ser Asn Gly 5250 5255 5260 ggc gcg gct gag cta gct cga ctg cga cat cta gac cac tgc cgc 27600 Gly Ala Ala Glu Leu Ala Arg Leu Arg His Leu Asp His Cys Arg 5265 5270 5275 cgc ttt cgc tgc ttt gcc cga gaa ctc acc gag ttc atc tac ttc 27645 Arg Phe Arg Cys Phe Ala Arg Glu Leu Thr Glu Phe Ile Tyr Phe 5280 5285 5290 gaa ata ccc gag gag cac cct caa gga ccg gcc cac gga gtg cgt 27690 Glu Ile Pro Glu Glu His Pro Gln Gly Pro Ala His Gly Val Arg 5295 5300 5305 att acc atc gaa ggg ggg ata gac tct cgc ctg cat cgg atc ttc 27735 Ile Thr Ile Glu Gly Gly Ile Asp Ser Arg Leu His Arg Ile Phe 5310 5315 5320 tgc cag cga ccc gtg cta atc gag cgc gac cag gga aac acc aca 27780 Cys Gln Arg Pro Val Leu Ile Glu Arg Asp Gln Gly Asn Thr Thr 5325 5330 5335 gtc tcc atc tac tgc atc tgt aac cac ccc gga ttg cat gaa agc 27825 Val Ser Ile Tyr Cys Ile Cys Asn His Pro Gly Leu His Glu Ser 5340 5345 5350 ctt tgc tgt ctt att tgt gct gag ttt aat aaa aac tgagttaaga 27871 Leu Cys Cys Leu Ile Cys Ala Glu Phe Asn Lys Asn 5355 5360 ctctcctacg gactaccaat tcttcaactc ggactttata acaatcagac cctccgttca 27931 agtcagaaga ccccaaccct tcctctgatc caggaatcta attctacctc cccagcacca 27991 cactttacta gccttcccga aactaacaac ctcggagctc aactgcacca cttttccaga 28051 agccttctct ctgccaatac taccactccc agaaccggag gtgagctccg tggtcttcct 28111 aataacaacc cctgggtggt aactgggttt gtaacgctag gtgtagttgc gggtgggctt 28171 gtgcttgtcc tttgctacct atacacacct tgctgtgctt atttagtaat cttgtgttgc 28231 tggtttaaga a atg ggg gcc cta cta gtc gcg ctt gct tta ctt tca 28278 Met Gly Ala Leu Leu Val Ala Leu Ala Leu Leu Ser 5365 5370 5375 ctt ttg gat ctg ggc tct act atg cta gtt cag cct gta cta ttt 28323 Leu Leu Asp Leu Gly Ser Thr Met Leu Val Gln Pro Val Leu Phe 5380 5385 5390 gat cca tgc ctc aat ttt gat cca gac aac tgc aca ctc act ttt 28368 Asp Pro Cys Leu Asn Phe Asp Pro Asp Asn Cys Thr Leu Thr Phe 5395 5400 5405 gct cca gag gct ggc cgc tgt gga gtt ctt att agg tgc gga cgg 28413 Ala Pro Glu Ala Gly Arg Cys Gly Val Leu Ile Arg Cys Gly Arg 5410 5415 5420 gaa tgc agt ccc att gaa ata cac cac aat aac aaa att tgg aac 28458 Glu Cys Ser Pro Ile Glu Ile His His Asn Asn Lys Ile Trp Asn 5425 5430 5435 aat acc tta ttc acc aca tgg cag cca gga gac cct gag tgg tat 28503 Asn Thr Leu Phe Thr Thr Trp Gln Pro Gly Asp Pro Glu Trp Tyr 5440 5445 5450 act gtc tct gtc cgt ggt cct gac ggt tcc atc cgc act gct aat 28548 Thr Val Ser Val Arg Gly Pro Asp Gly Ser Ile Arg Thr Ala Asn 5455 5460 5465 aac act ttt att ttt gct gag atg tgc gat ctg acc atg ttc atg 28593 Asn Thr Phe Ile Phe Ala Glu Met Cys Asp Leu Thr Met Phe Met 5470 5475 5480 agc aaa cag tat aac cta tgg cct cca agc aag gag aac att gtg 28638 Ser Lys Gln Tyr Asn Leu Trp Pro Pro Ser Lys Glu Asn Ile Val 5485 5490 5495 gca ttc tcc att gct tat ttc ttg tgt acg tgt ctc att act gct 28683 Ala Phe Ser Ile Ala Tyr Phe Leu Cys Thr Cys Leu Ile Thr Ala 5500 5505 5510 att cta tgt atc tgc ata cac ttg ctt att tgc cac cgc cac aga 28728 Ile Leu Cys Ile Cys Ile His Leu Leu Ile Cys His Arg His Arg 5515 5520 5525 aac agc aat gag gaa aaa gag aaa atg cct tgagcttttt ctcatttttg 28778 Asn Ser Asn Glu Glu Lys Glu Lys Met Pro 5530 5535 tttttttttg tttacagcc atg gct tca gtt ata gct cta att att gtc 28827 Met Ala Ser Val Ile Ala Leu Ile Ile Val 5540 5545 agc att ctc act gcc gca cag gga caa aca att gtc tat att acc 28872 Ser Ile Leu Thr Ala Ala Gln Gly Gln Thr Ile Val Tyr Ile Thr 5550 5555 5560 tta ggt cat aac cac act ctt ata gga ccc caa att agt tca cag 28917 Leu Gly His Asn His Thr Leu Ile Gly Pro Gln Ile Ser Ser Gln 5565 5570 5575 gtt ata tgg acc aaa ctt gga agt gtt gat tat ttt gac ata atc 28962 Val Ile Trp Thr Lys Leu Gly Ser Val Asp Tyr Phe Asp Ile Ile 5580 5585 5590 tgc aac aga act aaa cca ata ttt gta acc tgt aac aaa caa aat 29007 Cys Asn Arg Thr Lys Pro Ile Phe Val Thr Cys Asn Lys Gln Asn 5595 5600 5605 ctc acc tta att aat gtt agc gaa att tac agc ggt tac tat tat 29052 Leu Thr Leu Ile Asn Val Ser Glu Ile Tyr Ser Gly Tyr Tyr Tyr 5610 5615 5620 ggt tat gac aga cac agc agt gaa tat aaa aat tac cta gtt cgc 29097 Gly Tyr Asp Arg His Ser Ser Glu Tyr Lys Asn Tyr Leu Val Arg 5625 5630 5635 ata act caa ccc aaa acc aca aaa atg cca aat aag gca aaa att 29142 Ile Thr Gln Pro Lys Thr Thr Lys Met Pro Asn Lys Ala Lys Ile 5640 5645 5650 caa atg gtt agc gca tta gaa cat ctt aca tat ccc acc aca ccc 29187 Gln Met Val Ser Ala Leu Glu His Leu Thr Tyr Pro Thr Thr Pro 5655 5660 5665 gat gag aga aac att cca aat tca atg att gcc att att gcg gcg 29232 Asp Glu Arg Asn Ile Pro Asn Ser Met Ile Ala Ile Ile Ala Ala 5670 5675 5680 gtg gca gtg gga atg gca cta ata ata att tgt atg ttc cta tat 29277 Val Ala Val Gly Met Ala Leu Ile Ile Ile Cys Met Phe Leu Tyr 5685 5690 5695 gct tgt tac tgt aga aag ttt cat cac aaa cag gat tcc cta cta 29322 Ala Cys Tyr Cys Arg Lys Phe His His Lys Gln Asp Ser Leu Leu 5700 5705 5710 aat ttt tgacatttaa ttttttatac agct atg gtt tcc act aca gcc ttt 29373 Asn Phe Met Val Ser Thr Thr Ala Phe 5715 ttt gtt att agt agc ctt gca gct gtc act tat ggt cgc tca cac 29418 Phe Val Ile Ser Ser Leu Ala Ala Val Thr Tyr Gly Arg Ser His 5720 5725 5730 ctc act gta act gtt ggc tca act tgt aca cta caa gga ccc caa 29463 Leu Thr Val Thr Val Gly Ser Thr Cys Thr Leu Gln Gly Pro Gln 5735 5740 5745 gaa ggg cat gtc agt tgg tgg aga ata tat gat agt gga tgg ttc 29508 Glu Gly His Val Ser Trp Trp Arg Ile Tyr Asp Ser Gly Trp Phe 5750 5755 5760 att agg cca tgt gac cag cct ggt aac aaa ttt ttc tgc aac ggg 29553 Ile Arg Pro Cys Asp Gln Pro Gly Asn Lys Phe Phe Cys Asn Gly 5765 5770 5775 aga gac ttg acc att att aac atc aca gta aat gac cag ggc ttc 29598 Arg Asp Leu Thr Ile Ile Asn Ile Thr Val Asn Asp Gln Gly Phe 5780 5785 5790 tat tat gga act aac tat aaa aat aac tta gat tac aac att atc 29643 Tyr Tyr Gly Thr Asn Tyr Lys Asn Asn Leu Asp Tyr Asn Ile Ile 5795 5800 5805 gta gtg cca gcc acc act cca gct ccc cgc aaa acc act ttc ttt 29688 Val Val Pro Ala Thr Thr Pro Ala Pro Arg Lys Thr Thr Phe Phe 5810 5815 5820 agc agc agt gcc agt att tct aaa aca gct tct gca agc ttc aaa 29733 Ser Ser Ser Ala Ser Ile Ser Lys Thr Ala Ser Ala Ser Phe Lys 5825 5830 5835 aaa ttc gct tta cgt aat tcc aca acc tct tcc act tcc aat aat 29778 Lys Phe Ala Leu Arg Asn Ser Thr Thr Ser Ser Thr Ser Asn Asn 5840 5845 5850 aca atg tct aaa tca gta atc ggc atc gct gct gcc gcg ata gtg 29823 Thr Met Ser Lys Ser Val Ile Gly Ile Ala Ala Ala Ala Ile Val 5855 5860 5865 gga tta atg att ata att cta tgc ata atc tac tac gcc tgc tgc 29868 Gly Leu Met Ile Ile Ile Leu Cys Ile Ile Tyr Tyr Ala Cys Cys 5870 5875 5880 tat aga aaa caa cat gaa caa aaa acc gat ccc ttg ctg aat ttt 29913 Tyr Arg Lys Gln His Glu Gln Lys Thr Asp Pro Leu Leu Asn Phe 5885 5890 5895 gat att taattttttt atagaatc atg aaa aaa cta agt atc cta gct 29961 Asp Ile Met Lys Lys Leu Ser Ile Leu Ala 5900 5905 ttc att ttg ttt caa aca ttt acc aat gtg cag act act tta agt 30006 Phe Ile Leu Phe Gln Thr Phe Thr Asn Val Gln Thr Thr Leu Ser 5910 5915 5920 cat ggt ata gag aac cac act acc tct tat gag ctc aca aac att 30051 His Gly Ile Glu Asn His Thr Thr Ser Tyr Glu Leu Thr Asn Ile 5925 5930 5935 act acc cat cat cct aaa tat gct atg caa cta gaa atc acc atg 30096 Thr Thr His His Pro Lys Tyr Ala Met Gln Leu Glu Ile Thr Met 5940 5945 5950 cta att gta gtt gga ata ctt atc cta gct att att ttc tat ttt 30141 Leu Ile Val Val Gly Ile Leu Ile Leu Ala Ile Ile Phe Tyr Phe 5955 5960 5965 aca cta tgc cgc caa ata cct aat att cat aaa aat tct aaa aga 30186 Thr Leu Cys Arg Gln Ile Pro Asn Ile His Lys Asn Ser Lys Arg 5970 5975 5980 cgt ccc atc tat tgc cct gtg att agt cga ccc cat atg act cta 30231 Arg Pro Ile Tyr Cys Pro Val Ile Ser Arg Pro His Met Thr Leu 5985 5990 5995 aat gaa atc taagatcatc tatttctctt ttttacagta tggtgaacac 30280 Asn Glu Ile 6000 caatcatgat tcctagaaat ttcttcttca ccatactcat ctgtgctttt aatgtctgtg 30340 ccaccttcac agcagtagcc actgcaaccc cagactgtat aggagcattt gcctcatata 30400 cacttttcgc ttttgtcgct tgcacctgcg tgtgtagcgt agtctgcctg gttattaatt 30460 ttttccaact tgtagactgg atctttgtac gacttgccta cctgcgtcac catcccgaat 30520 accgcaatca ac atg ttg cgg cac ttc tca gac tta ttt aaa acc atg 30568 Met Leu Arg His Phe Ser Asp Leu Phe Lys Thr Met 6005 6010 cag gct ata cta cca gtc att ctg ctt ctg ttg ctc ccc tgc gat 30613 Gln Ala Ile Leu Pro Val Ile Leu Leu Leu Leu Leu Pro Cys Asp 6015 6020 6025 gcc tta acc ccc gtc gct aat cgt acc cca cct gaa caa ctt aga 30658 Ala Leu Thr Pro Val Ala Asn Arg Thr Pro Pro Glu Gln Leu Arg 6030 6035 6040 aaa tgc aaa ttc caa caa cca tgg aca ttc ctt gat tgc tac cga 30703 Lys Cys Lys Phe Gln Gln Pro Trp Thr Phe Leu Asp Cys Tyr Arg 6045 6050 6055 gaa aaa tct gat ttc cct aca tac tgg att atg atc att gga att 30748 Glu Lys Ser Asp Phe Pro Thr Tyr Trp Ile Met Ile Ile Gly Ile 6060 6065 6070 gtc aat cta gtt tct tgc aca cta ttc tct ttc ctt gtt tat cat 30793 Val Asn Leu Val Ser Cys Thr Leu Phe Ser Phe Leu Val Tyr His 6075 6080 6085 ttt ttt gat ttt gga tgg aat gcc ccc aat gca ctc act tac cca 30838 Phe Phe Asp Phe Gly Trp Asn Ala Pro Asn Ala Leu Thr Tyr Pro 6090 6095 6100 caa gaa cca gag gaa cat atc cca cta cag aac atg caa cag cca 30883 Gln Glu Pro Glu Glu His Ile Pro Leu Gln Asn Met Gln Gln Pro 6105 6110 6115 ata gct ata ata gat tat gac aat gag cca cag ccc tcg ctg ctt 30928 Ile Ala Ile Ile Asp Tyr Asp Asn Glu Pro Gln Pro Ser Leu Leu 6120 6125 6130 cct gct att agt tac ttc aac cta acc ggt gga gat gac tgacccactc 30977 Pro Ala Ile Ser Tyr Phe Asn Leu Thr Gly Gly Asp Asp 6135 6140 6145 gccgcctcca ctgctgccga ggaactactt gatatggacg gccgcgcctc agaacagcga 31037 ctcgcccaac tacgcattcg ccagcagcag gaacgtgccg ccaaggagct cagggatgct 31097 attgagattc accagtgcaa aaaaggcata ttctgcttgg taaaacaagc caagatctcc 31157 tacgagatca ccgctaacga ccaccgcctc tcatatgagc ttggcccgca gcgtcagaaa 31217 ttcacttgca tggtgggaat caaccccata gtcatcaccc agcaagccgg agataccaag 31277 ggttgcatcc attgttcctg tgaatccacc gagtgcatct acaccctact gaagaccctc 31337 tgcggccttc gagacctcct acccatgaac taatcacccc cgcccctacc aattacaaaa 31397 agccaattaa taaaaatcac ttacttaaaa tcagaaataa ggtttttgtc tgcgttgttt 31457 tcaagcagca cctcacttcc ctcttcccaa ctctggtact ctaagcctcg gcgggtggca 31517 tacttcctcc acactttgaa agggatgtca aattttagtt cctcttctct gcccacaatc 31577 ttcatttctt tatccccag atg gcc aaa cga gtt cga cta agc agc tcc 31626 Met Ala Lys Arg Val Arg Leu Ser Ser Ser 6150 6155 ttc aat ccg gtc tac ccc tat gaa gat gaa agc agc tca caa cac 31671 Phe Asn Pro Val Tyr Pro Tyr Glu Asp Glu Ser Ser Ser Gln His 6160 6165 6170 ccc ttt ata aac cct ggt ttc att tcc tca aat ggt ttt aca caa 31716 Pro Phe Ile Asn Pro Gly Phe Ile Ser Ser Asn Gly Phe Thr Gln 6175 6180 6185 agt cca gac gga gtt ctt acg ctc aaa tgt gtt gcc cct ctt act 31761 Ser Pro Asp Gly Val Leu Thr Leu Lys Cys Val Ala Pro Leu Thr 6190 6195 6200 acc acc agc ggc gct cta gac atc aag gtg gga gga ggg ctt aaa 31806 Thr Thr Ser Gly Ala Leu Asp Ile Lys Val Gly Gly Gly Leu Lys 6205 6210 6215 gta gac agc act gat ggt tcc tta gaa gaa gac atg ggc att gca 31851 Val Asp Ser Thr Asp Gly Ser Leu Glu Glu Asp Met Gly Ile Ala 6220 6225 6230 gct ccc ctt acc aaa gtt aac cac tcc gta gga tta gca tta ggt 31896 Ala Pro Leu Thr Lys Val Asn His Ser Val Gly Leu Ala Leu Gly 6235 6240 6245 gac ggg cta gag aca aaa gaa aac aaa ctt tat gta aaa ctg gga 31941 Asp Gly Leu Glu Thr Lys Glu Asn Lys Leu Tyr Val Lys Leu Gly 6250 6255 6260 gaa gga ctt aaa ttt aac tct ggt agt ata aac att gac cat gat 31986 Glu Gly Leu Lys Phe Asn Ser Gly Ser Ile Asn Ile Asp His Asp 6265 6270 6275 att aac acc tta tgg acg gga gtt aat cca agt gct aac tgt ata 32031 Ile Asn Thr Leu Trp Thr Gly Val Asn Pro Ser Ala Asn Cys Ile 6280 6285 6290 att acg gaa gat gga gaa gct aat gac agc aag ctc acc cta ata 32076 Ile Thr Glu Asp Gly Glu Ala Asn Asp Ser Lys Leu Thr Leu Ile 6295 6300 6305 ctt gtt aag aca ggc gga cta gtt aat gct tat gtc tca tta atg 32121 Leu Val Lys Thr Gly Gly Leu Val Asn Ala Tyr Val Ser Leu Met 6310 6315 6320 ggt gac tca gaa gcg gtc aat aaa cta acc aca gat aaa agt gct 32166 Gly Asp Ser Glu Ala Val Asn Lys Leu Thr Thr Asp Lys Ser Ala 6325 6330 6335 caa att act gtt gat ata tac ttt gat aat gaa gga aaa gtt ctt 32211 Gln Ile Thr Val Asp Ile Tyr Phe Asp Asn Glu Gly Lys Val Leu 6340 6345 6350 act gaa cta tca gca ctt aaa aca ggt ctt aaa cat aaa ttt ggt 32256 Thr Glu Leu Ser Ala Leu Lys Thr Gly Leu Lys His Lys Phe Gly 6355 6360 6365 caa aat atg gct tct gac gaa gca caa aac tgc aaa ggc ttt atg 32301 Gln Asn Met Ala Ser Asp Glu Ala Gln Asn Cys Lys Gly Phe Met 6370 6375 6380 ccc agc tta act gca tac cca ttt aga aat cca act aaa cct acc 32346 Pro Ser Leu Thr Ala Tyr Pro Phe Arg Asn Pro Thr Lys Pro Thr 6385 6390 6395 aaa gga aga gaa gac tac atc tat gga atc act tac tat caa gcc 32391 Lys Gly Arg Glu Asp Tyr Ile Tyr Gly Ile Thr Tyr Tyr Gln Ala 6400 6405 6410 aca gat ggc aca ctc tat gag cta aaa acc act gtt act cta aac 32436 Thr Asp Gly Thr Leu Tyr Glu Leu Lys Thr Thr Val Thr Leu Asn 6415 6420 6425 tac agt gtt att agt tct cta tgt gca tat gca atg cac att tca 32481 Tyr Ser Val Ile Ser Ser Leu Cys Ala Tyr Ala Met His Ile Ser 6430 6435 6440 tgg tca tgg gat agt gta aca gag cca gag aca acc ccc act act 32526 Trp Ser Trp Asp Ser Val Thr Glu Pro Glu Thr Thr Pro Thr Thr 6445 6450 6455 ctt att acc tcc ccc ttc tcc ttt tcc tac att aga gaa gat gac 32571 Leu Ile Thr Ser Pro Phe Ser Phe Ser Tyr Ile Arg Glu Asp Asp 6460 6465 6470 tgacaaagaa taaagttcaa cttttttatt gaaaatcagt ttacaggata cgagtagtta 32631 ttttgcctcc cccttcccat ttcatagaat acaccaatct ctccccacgc acagctttaa 32691 acatttggat tccatttgag atagtcatgg atttagattc cacattccac acagtttcag 32751 agctagataa tcttggatca gtgatagata taaatccatc ggggcagtcc ttcaaggtga 32811 tttcacagtc cagttgctgt ggctgcggct ccggagtctg gatcagagtc atctggaaga 32871 agaacgatgg gagtcataat ccgagaacgg gatcgggcgg ttgtgtctca tcaaaccccg 32931 aagcagtcgc tgtctgcgcc gctccgtgcg actgctgctg atgggatcgg ggtccacagt 32991 ctctcgaagc atgattctaa tagccctcaa cattaacatt ctggtacgat gcgcacagca 33051 acgcatcctg atctcactta ggtcacagca gtaagtacaa cacaacacca caatgttgtt 33111 taacaggcca taattaaagg cgctccagcc aaaactcatt tcaggaataa tttgcccagc 33171 gtggccatcg taccaaatcc tgatgtaaat caaatggcgc cccctccaga acacactgcc 33231 cacatacatg atctccttag gcatatgcat attcacaatc tctcggtacc atggacagcg 33291 ctggttaatc atgcagcccc aaataatctt ccggaaccaa atggccagca ctgcgccccc 33351 agcaatacat tgaagagaac cctgtcgatt acagtgacaa tggagaaccc acttctctcg 33411 cccatggatc acttgggaat aaaatatatc tattgtggca caacacagac ataaatgcat 33471 acatcttctc atcaccctta actcttcagg ggttaaaaac atatcccagg gaataggaag 33531 ctcttgcaaa acagtgaagg tggcagaaca aggcagaccg cgaacataac ttacactgtg 33591 catggtcaag gtattgcaat ctggtaacag cggatgctcc tcagtcatag aagctctggt 33651 ttcactttcc tcacagcgtg gtaaaggggc cctcagttga ggttccctgg tgtaaggatg 33711 gtgtctggcg cacgatgtcg agcgtgcccg cgacctcgtt gtaatggagc ttcttcctga 33771 cattctcgta ttttgcaaag cagaacctag tcttggcaca gcacacgtcc cgtcgcctcc 33831 tgtcccgccg cctagcacgt tcagtgtggt aattatagta cagccattcc cgtagattgg 33891 tcaaaagatc ttcagcctca gttgtcataa aaactccatc atatcttact gctctgataa 33951 aatcattcac ggtagaaagt gcaatgccca gccaggcaat gcaattagct tgtgtttcga 34011 ccaaaggagg gggaggaaga catggaagaa ccataattaa tttttatgcc agacgatccc 34071 gcagtatttc tatatggaga tcacggagat ggcacctctc gcccccactg tgttgatgaa 34131 aaatgacagc taggtcaaac ataatgcgat tttccaggtg ctcaacggtg gcttcaagca 34191 aagcctccaa acgtacatcc aaaaacaaaa gaacagcaaa agcaggagca ttttctaatt 34251 cctcaatcat catattacat tcctgtacca ttcccaaata attttcatct ttccatcctt 34311 gaattattcg tgttatttca tctggtaaat ccaatccaca catgagaaat agctcccgga 34371 gggcgccctc caccggcaat cttaagcata ccctcatagt gacaaaatat cgtgctcctc 34431 tgtcacctgc agcaaattga gaatggcaat atcaaacgga atgccactgg ctctaagttc 34491 ttctctaagt tccagttgta aaaactcttg catatcatcg ccaaactgct tagccatagg 34551 tcctccagga ataagagcgg gggacgctac agtgcagaac aagcgcatgc cgccccaatt 34611 gcctccagca aaagtgaggt tgcaatatgc atactgagaa cctccagtga tatcatccag 34671 tgtactggaa agataatcag gcagagcttc tcgtatgcaa ttaataatag aaaagtctgc 34731 cagatgcaca tttaaagcct gtgggatgca gatgcaataa gttatcgcgc tgcgctccaa 34791 cattgttagt atggttagtc tgtaaaaaca aaaaacaaaa aaaaaattac atcacgctag 34851 actggcgaac gggtggaaaa atcactctct ccaacaccag gcaggctaca gggtctccag 34911 cgcgaccctc gtaaaacctg tcagtatgat taaaaagcat caccgaaagg ggttgttgat 34971 ggccagcata tattatttgc gatgaagcat acaatccaga agtgttagta tcagttaaag 35031 aaaaaaatcg gccaagatag catctcggaa cgattatgct caatctcaaa tgcagcaaag 35091 cgacacctcg cggatgcaaa ataaaatcca caggagcata aaaaaagtaa ttattcccct 35151 cttgcacagg cagcctagct cccggcccct ccaaaatcac atataaagct tcagcagcca 35211 tagcttaccg cgcaaatcag gcacagcagt cagatagaga aaaagctgtg aactgactgc 35271 ccagcctgtg cgcaatatat agagaaccct tacactgacg taattggaca aagtctaaaa 35331 aatcccgcca aaaaaccagc acacgcccag aactgtgtca cccgctaaaa aaaataattt 35391 tcacttcctc gttccgtgaa tgacgtcagt tcccctttcc cacgagccgt cacttccggt 35451 catcttgcaa cgtcacctcc ccgcgccggc ccgccccttt tgaccgttga accctgtagc 35511 caatcccctt ccgccctcca ttttcaaaag ctcatttgca tgttggcacc gttccattta 35571 taaggtatat tattgatgat g 35592 <210> 40 <211> 495 <212> PRT <213> Simian adenovirus 27 <400> 40 Met Asp Pro Thr Asn Pro Leu Gln Gln Gly Ile Arg Phe Gly Phe His 1 5 10 15 Ser Ser Ser Phe Val Glu Asn Met Glu Gly Ser Gln Ala Glu Asp Asn 20 25 30 Leu Arg Leu Leu Ala Ser Ala Ala Ser Gly Arg Ser Gly Asp Pro Glu 35 40 45 Thr Pro Thr Gly His Ala Ser Gly Ser Gly Gly Gly Ala Ala Gly Gly 50 55 60 Gln Pro Glu Ser Arg Pro Gly Pro Ser Gly Gly Gly Gly Gly Gly Val 65 70 75 80 Ala Asp Leu Phe Pro Glu Leu Arg Arg Val Leu Thr Arg Ser Thr Ser 85 90 95 Ser Gly Gln Asp Arg Gly Ile Lys Arg Glu Arg Asn Ala Ser Gly His 100 105 110 Asn Ser Arg Thr Glu Leu Ala Leu Ser Leu Met Ser Arg Arg Arg Pro 115 120 125 Glu Thr Val Trp Trp His Glu Val Gln Ser Glu Gly Arg Asp Glu Val 130 135 140 Ser Ile Leu Gln Glu Lys Tyr Ser Leu Glu Gln Leu Lys Thr Cys Trp 145 150 155 160 Leu Glu Pro Glu Asp Asp Trp Glu Val Ala Ile Arg Asn Tyr Ala Lys 165 170 175 Ile Ser Leu Arg Pro Asp Lys Gln Tyr Arg Ile Thr Lys Lys Ile Asn 180 185 190 Ile Arg Asn Ala Cys Tyr Ile Ser Gly Asn Gly Ala Glu Val Ile Ile 195 200 205 Asp Thr Gln Asp Lys Thr Ala Phe Arg Cys Cys Met Met Gly Met Trp 210 215 220 Pro Gly Val Ala Gly Met Glu Ala Val Thr Leu Met Asn Ile Arg Phe 225 230 235 240 Arg Gly Asp Gly Tyr Asn Gly Ile Val Phe Met Ala Asn Thr Lys Leu 245 250 255 Ile Leu His Gly Cys Ser Phe Phe Gly Phe Asn Asn Thr Cys Val Glu 260 265 270 Ala Trp Gly Gln Val Ser Val Arg Gly Cys Ser Phe Tyr Ala Gly Trp 275 280 285 Ile Ala Leu Ser Gly Arg Thr Lys Ser Gln Leu Ser Val Lys Lys Cys 290 295 300 Met Phe Glu Arg Cys Asn Leu Gly Ile Leu Asn Glu Gly Glu Ala Arg 305 310 315 320 Val Arg His Cys Ala Ala Thr Glu Thr Gly Cys Phe Ile Leu Ile Lys 325 330 335 Gly Asn Ala Ser Val Lys His Asn Met Ile Cys Gly Pro Ser Asp Glu 340 345 350 Arg Pro Tyr Gln Met Leu Thr Cys Ala Gly Gly His Cys Asn Met Leu 355 360 365 Ala Thr Val His Ile Val Ser His Ala Arg Lys Lys Trp Pro Val Phe 370 375 380 Glu His Asn Val Met Thr Lys Cys Thr Met His Ile Gly Gly Arg Arg 385 390 395 400 Gly Met Phe Met Pro Tyr Gln Cys Asn Leu Asn His Val Lys Val Met 405 410 415 Leu Glu Pro Asp Ala Phe Ser Arg Met Ser Leu Thr Gly Ile Phe Asp 420 425 430 Met Asn Val Gln Leu Trp Lys Ile Leu Arg Tyr Asp Asp Thr Lys Ser 435 440 445 Arg Val Arg Ala Cys Glu Cys Gly Gly Lys His Ala Arg Phe Gln Pro 450 455 460 Val Cys Val Asp Val Thr Glu Asp Leu Arg Pro Asp His Leu Val Leu 465 470 475 480 Ala Cys Thr Gly Ala Glu Phe Gly Ser Ser Gly Glu Glu Thr Asp 485 490 495 <210> 41 <211> 138 <212> PRT <213> Simian adenovirus 27 <400> 41 Met Ser Gly Ser Ala Ser Phe Glu Gly Gly Val Phe Ser Pro Tyr Leu 1 5 10 15 Thr Gly Arg Leu Pro Pro Trp Ala Gly Val Arg Gln Asn Val Met Gly 20 25 30 Ser Thr Val Asp Gly Arg Pro Val Gln Pro Ala Asn Ser Ser Thr Leu 35 40 45 Thr Tyr Ala Thr Leu Ser Ser Ser Pro Leu Asp Ala Ala Ala Ala Ala 50 55 60 Ala Ala Ser Ala Ala Ala Asn Thr Val Leu Gly Met Gly Tyr Tyr Gly 65 70 75 80 Ser Ile Val Ala Asn Ser Ser Ser Ser Asn Asn Pro Ser Thr Leu Ala 85 90 95 Glu Asp Lys Leu Leu Val Leu Leu Ala Gln Leu Glu Ala Leu Thr Gln 100 105 110 Arg Leu Gly Glu Leu Ser Gln Gln Val Ala Gln Leu Arg Glu Gln Thr 115 120 125 Glu Ser Ala Val Ala Thr Ala Lys Ser Lys 130 135 <210> 42 <211> 387 <212> PRT <213> Simian adenovirus 27 <400> 42 Met His Pro Val Leu Arg Gln Met Arg Pro Gln Gln Gln Ala Pro Ser 1 5 10 15 Gln Gln Gln Gln Gln Pro Gln Lys Ala Leu Pro Ala Pro Ala Thr Thr 20 25 30 Ala Ala Ala Ala Val Cys Gly Ala Gly Gln Pro Ala Tyr Asp Leu Asp 35 40 45 Leu Glu Glu Gly Glu Gly Leu Ala Arg Leu Gly Ala Pro Ser Pro Glu 50 55 60 Arg His Pro Arg Val Gln Leu Lys Lys Asp Ser Arg Glu Ala Tyr Val 65 70 75 80 Pro Gln His Asn Leu Phe Arg Asp Arg Ser Gly Glu Glu Pro Glu Glu 85 90 95 Met Arg Ala Ser Arg Phe Asn Ala Gly Arg Glu Leu Arg His Gly Leu 100 105 110 Asp Arg Arg Arg Leu Leu Arg Asp Glu Asp Phe Glu Val Asp Glu Val 115 120 125 Thr Gly Ile Ser Pro Ala Arg Ala His Val Ala Ala Ala Asn Leu Val 130 135 140 Ser Ala Tyr Glu Gln Thr Val Lys Glu Glu Arg Asn Phe Gln Lys Ser 145 150 155 160 Phe Asn Asn His Val Arg Thr Leu Ile Ala Arg Glu Glu Val Thr Leu 165 170 175 Gly Leu Met His Leu Trp Asp Leu Met Glu Ala Ile Thr Gln Asn Pro 180 185 190 Thr Ser Lys Pro Leu Thr Ala Gln Leu Phe Leu Val Val Gln His Ser 195 200 205 Arg Asp Asn Glu Ala Phe Arg Glu Ala Leu Leu Asn Ile Thr Glu Pro 210 215 220 Glu Gly Arg Trp Leu Tyr Asp Leu Ile Asn Ile Leu Gln Ser Ile Ile 225 230 235 240 Val Gln Glu Arg Ser Leu Gly Leu Ala Glu Lys Val Ala Ala Ile Asn 245 250 255 Tyr Ser Val Leu Ser Leu Gly Lys Tyr Tyr Ala Arg Lys Ile Tyr Lys 260 265 270 Thr Pro Tyr Val Pro Ile Asp Lys Glu Val Lys Ile Asp Gly Phe Tyr 275 280 285 Met Arg Met Thr Leu Lys Val Leu Thr Leu Ser Asp Asp Leu Gly Val 290 295 300 Tyr Arg Asn Asp Arg Met His Arg Ala Val Ser Ala Ser Arg Arg Arg 305 310 315 320 Glu Leu Ser Asp Arg Glu Leu Met His Ser Leu Gln Arg Ala Leu Thr 325 330 335 Gly Ala Gly Thr Asp Gly Glu Asn Tyr Phe Asp Met Gly Ala Asp Leu 340 345 350 Gln Trp Gln Pro Ser Arg Arg Ala Leu Asp Ala Ala Gly Cys Glu Leu 355 360 365 Pro Tyr Val Glu Glu Val Asp Glu Gly Glu Glu Glu Glu Gly Glu Tyr 370 375 380 Leu Glu Asp 385 <210> 43 <211> 587 <212> PRT <213> Simian adenovirus 27 <400> 43 Met Glu Gln Gln Ala Pro Asp Pro Ala Met Arg Ala Ala Leu Gln Ser 1 5 10 15 Gln Pro Ser Gly Ile Asn Ser Ser Asp Asp Trp Thr Gln Ala Met Gln 20 25 30 Arg Ile Met Ala Leu Thr Thr Arg Asn Pro Glu Ala Phe Arg Gln Gln 35 40 45 Pro Gln Ala Asn Arg Leu Ser Ala Ile Leu Glu Ala Val Val Pro Ser 50 55 60 Arg Ser Asn Pro Thr His Glu Lys Val Leu Ala Ile Val Asn Ala Leu 65 70 75 80 Val Glu Asn Lys Ala Ile Arg Pro Asp Glu Ala Gly Leu Val Tyr Asn 85 90 95 Ala Leu Leu Glu Arg Val Ala Arg Tyr Asn Ser Ser Asn Val Gln Thr 100 105 110 Asn Leu Asp Arg Met Val Thr Asp Val Arg Glu Ala Val Ser Gln Arg 115 120 125 Glu Arg Phe Arg Arg Asp Ala Asn Leu Gly Ser Leu Val Ala Leu Asn 130 135 140 Ala Phe Leu Ser Thr Gln Pro Ala Asn Val Pro Arg Gly Gln Gln Asp 145 150 155 160 Tyr Thr Asn Phe Leu Ser Ala Leu Arg Leu Met Val Thr Glu Val Pro 165 170 175 Gln Ser Glu Val Tyr Gln Ser Gly Pro Asp Tyr Phe Phe Gln Thr Ser 180 185 190 Arg Gln Gly Leu Gln Thr Val Asn Leu Ser Gln Ala Phe Lys Asn Leu 195 200 205 Arg Gly Leu Trp Gly Val His Ala Pro Val Gly Asp Arg Ala Thr Val 210 215 220 Ser Ser Leu Leu Thr Pro Asn Ser Arg Leu Leu Leu Leu Leu Val Ser 225 230 235 240 Pro Phe Thr Asp Ser Gly Ser Ile Asp Arg Asn Ser Tyr Leu Gly Tyr 245 250 255 Leu Leu Asn Leu Tyr Arg Glu Ala Ile Gly Gln Ser Gln Val Asp Glu 260 265 270 Gln Thr Tyr Gln Glu Ile Thr Gln Val Ser Arg Ala Leu Gly Gln Glu 275 280 285 Asp Thr Gly Ser Leu Glu Ala Thr Leu Asn Phe Leu Leu Thr Asn Arg 290 295 300 Ser Gln Lys Ile Pro Pro Gln Tyr Ala Leu Thr Ala Glu Glu Glu Arg 305 310 315 320 Ile Leu Arg Tyr Val Gln Gln Ser Val Gly Leu Phe Leu Met Gln Glu 325 330 335 Gly Ala Thr Pro Thr Ala Ala Leu Asp Met Thr Ala Arg Asn Met Glu 340 345 350 Pro Ser Met Tyr Ala Ser Asn Arg Pro Phe Ile Asn Lys Leu Leu Asp 355 360 365 Tyr Leu His Arg Ala Ala Ala Met Asn Ser Asp Tyr Phe Thr Asn Ala 370 375 380 Ile Leu Asn Pro His Trp Leu Pro Pro Pro Gly Phe Tyr Thr Gly Glu 385 390 395 400 Tyr Asp Met Pro Asp Pro Asn Asp Gly Phe Leu Trp Asp Asp Val Asp 405 410 415 Ser Ser Ile Phe Ser Pro Pro Pro Gly Tyr Asn Thr Trp Lys Lys Glu 420 425 430 Gly Gly Asp Arg Arg His Ser Ser Val Ser Leu Ser Gly Ser Arg Gly 435 440 445 Ala Ala Ala Ala Val Pro Glu Ala Ala Ser Pro Phe Pro Ser Leu Pro 450 455 460 Phe Ser Leu Asn Ser Val Arg Ser Ser Glu Leu Gly Arg Ile Thr Arg 465 470 475 480 Pro Arg Leu Met Gly Glu Glu Glu Tyr Leu Asn Asp Ser Leu Leu Arg 485 490 495 Pro Glu Arg Glu Lys Asn Phe Pro Asn Asn Gly Ile Glu Ser Leu Val 500 505 510 Asp Lys Met Ser Arg Trp Lys Thr Tyr Ala Gln Asp His Lys Asp Glu 515 520 525 Pro Arg Ile Leu Gly Ala Ala Ser Gly Thr Thr Arg Arg Arg Gln Arg 530 535 540 His Asp Arg Gln Arg Gly Leu Val Trp Asp Asp Glu Asp Ser Ala Asp 545 550 555 560 Asp Ser Ser Val Leu Asp Leu Gly Gly Arg Gly Gly Gly Asn Pro Phe 565 570 575 Ala His Leu Arg Pro His Phe Gly Arg Met Leu 580 585 <210> 44 <211> 563 <212> PRT <213> Simian adenovirus 27 <400> 44 Met Met Arg Arg Ala Val Leu Gly Gly Ala Val Val Tyr Pro Glu Gly 1 5 10 15 Pro Pro Pro Ser Tyr Glu Ser Val Met Gln Gln Gln Ala Ala Ala Val 20 25 30 Met Gln Pro Ser Leu Glu Ala Pro Phe Val Pro Pro Arg Tyr Leu Ala 35 40 45 Pro Thr Glu Gly Arg Asn Ser Ile Arg Tyr Ser Glu Leu Ala Pro Gln 50 55 60 Tyr Asp Thr Thr Arg Leu Tyr Leu Val Asp Asn Lys Ser Ala Asp Ile 65 70 75 80 Ala Ser Leu Asn Tyr Gln Asn Asp His Ser Asn Phe Leu Thr Thr Val 85 90 95 Val Gln Asn Asn Asp Phe Thr Pro Thr Glu Ala Ser Thr Gln Thr Ile 100 105 110 Asn Phe Asp Glu Arg Ser Arg Trp Gly Gly Gln Leu Lys Thr Ile Met 115 120 125 His Thr Asn Met Pro Asn Val Asn Glu Tyr Met Phe Ser Asn Lys Phe 130 135 140 Lys Ala Arg Val Met Val Ser Arg Lys Ala Pro Glu Gly Val Thr Val 145 150 155 160 Ala Asp Asn Tyr Asp His Lys Gln Asp Ile Leu Glu Tyr Glu Trp Phe 165 170 175 Glu Phe Thr Leu Pro Glu Gly Asn Phe Ser Ala Thr Met Thr Ile Asp 180 185 190 Leu Met Asn Asn Ala Ile Ile Asp Asn Tyr Leu Glu Val Gly Arg Gln 195 200 205 Asn Gly Val Met Glu Ser Asp Ile Gly Val Lys Phe Asp Thr Arg Asn 210 215 220 Phe Arg Leu Gly Trp Asp Pro Lys Thr Lys Leu Ile Met Pro Gly Val 225 230 235 240 Tyr Thr Tyr Glu Ala Phe His Pro Asp Ile Val Leu Leu Pro Gly Cys 245 250 255 Gly Val Asp Phe Thr Glu Ser Arg Leu Ser Asn Leu Leu Gly Ile Arg 260 265 270 Lys Arg His Pro Phe Gln Glu Gly Phe Lys Ile Leu Tyr Glu Asp Leu 275 280 285 Glu Gly Gly Asn Ile Pro Ala Leu Leu Asp Val Glu Ala Tyr Glu Lys 290 295 300 Ser Lys Lys Glu Gln Glu Ala Lys Thr Glu Ala Ala Lys Ala Ala Ala 305 310 315 320 Val Ala Lys Ala Asn Ile Val Ala Ser Asp Pro Val Arg Val Ala Asn 325 330 335 Ala Glu Glu Val Arg Gly Asp Asn Tyr Thr Ala Ser Ala Val Ala Thr 340 345 350 Glu Glu Ser Leu Leu Ala Ala Val Ala Glu Asn Glu Thr Thr Glu Thr 355 360 365 Lys Leu Thr Ile Gln Pro Val Glu Lys Asp Ser Lys Ser Arg Ser Tyr 370 375 380 Asn Val Leu Asp Asp Lys Ile Asn Thr Ala Tyr Arg Ser Trp Tyr Leu 385 390 395 400 Ser Tyr Asn Tyr Gly Asp Pro Glu Lys Gly Val Arg Ser Trp Thr Leu 405 410 415 Leu Thr Thr Ser Asp Val Thr Cys Gly Ala Glu Gln Val Tyr Trp Ser 420 425 430 Leu Pro Asp Met Met Gln Asp Pro Val Thr Phe Arg Ser Thr Arg Gln 435 440 445 Val Ser Asn Tyr Pro Val Val Gly Ala Glu Leu Met Pro Val Phe Ser 450 455 460 Lys Ser Phe Tyr Asn Glu Gln Ala Val Tyr Ser Gln Gln Leu Arg Gln 465 470 475 480 Ser Thr Ser Leu Thr His Val Phe Asn Arg Phe Pro Glu Asn Gln Ile 485 490 495 Leu Ile Arg Pro Pro Ala Pro Thr Ile Thr Thr Val Ser Glu Asn Val 500 505 510 Pro Ala Leu Thr Asp His Gly Thr Leu Pro Leu Arg Ser Ser Ile Arg 515 520 525 Gly Val Gln Arg Val Thr Val Thr Asp Ala Arg Arg Arg Thr Cys Pro 530 535 540 Tyr Val Tyr Lys Ala Leu Gly Ile Val Ala Pro Arg Val Leu Ser Ser 545 550 555 560 Arg Thr Phe <210> 45 <211> 192 <212> PRT <213> Simian adenovirus 27 <400> 45 Met Ser Ile Leu Ile Ser Pro Ser Asn Asn Thr Gly Trp Gly Leu Arg 1 5 10 15 Thr Pro Thr Arg Met Tyr Gly Gly Ala Arg Lys Arg Ser Thr Gln His 20 25 30 Pro Val Arg Val Arg Gly His Phe Arg Ala Pro Trp Gly Ala Leu Lys 35 40 45 Gly Arg Ala Arg Thr Arg Thr Thr Val Asp Asp Val Ile Asp Gln Val 50 55 60 Val Ala Asp Ala Arg Asn Tyr Thr Pro Ala Ala Pro Thr Ser Thr Val 65 70 75 80 Asp Ala Val Ile Asp Ser Val Val Ala Asp Ala Arg Asp Tyr Ala Arg 85 90 95 Arg Lys Ser Arg Arg Arg Arg Ile Ala Arg Arg His Arg Ala Thr Pro 100 105 110 Ala Met Arg Ala Ala Arg Ala Leu Leu Arg Arg Ala Lys Arg Val Gly 115 120 125 Arg Arg Ala Met Leu Arg Ala Ala Arg Arg Ala Ala Ser Gly Ala Ser 130 135 140 Ala Gly Arg Ser Arg Arg Arg Ala Ala Thr Ala Ala Ala Ala Ala Ile 145 150 155 160 Ala Asn Met Ala Gln Pro Arg Arg Gly Asn Val Tyr Trp Val Arg Asp 165 170 175 Ala Thr Thr Gly Gln Arg Val Pro Val Arg Thr Arg Pro Pro Arg Thr 180 185 190 <210> 46 <211> 350 <212> PRT <213> Simian adenovirus 27 <400> 46 Met Ser Lys Arg Lys Tyr Lys Glu Glu Met Leu Gln Val Ile Ala Pro 1 5 10 15 Glu Ile Tyr Gly Pro Pro Val Lys Asp Glu Lys Lys Pro Arg Lys Ile 20 25 30 Lys Arg Val Lys Lys Asp Lys Lys Glu Glu Asp Gly Asp Asp Gly Leu 35 40 45 Val Glu Phe Val Arg Glu Phe Ala Pro Arg Arg Arg Val Gln Trp Arg 50 55 60 Gly Arg Arg Val Arg Pro Val Leu Arg Pro Gly Thr Thr Val Val Phe 65 70 75 80 Thr Pro Gly Glu Arg Ser Ser Thr Thr Phe Lys Arg Ser Tyr Asp Glu 85 90 95 Val Tyr Gly Asp Asp Asp Ile Leu Glu Gln Ala Ala Asp Arg Leu Gly 100 105 110 Glu Phe Ala Tyr Gly Lys Arg Ser Arg Ser Ser Pro Lys Glu Glu Ala 115 120 125 Val Ser Ile Pro Leu Asp His Gly Asn Pro Thr Pro Ser Leu Lys Pro 130 135 140 Val Thr Leu Gln Gln Val Leu Pro Val Pro Pro Arg Arg Gly Val Lys 145 150 155 160 Arg Glu Gly Glu Asp Leu Tyr Pro Thr Met Gln Leu Met Val Pro Lys 165 170 175 Arg Gln Lys Leu Glu Asp Val Leu Glu Lys Met Lys Val Asp Pro Asp 180 185 190 Ile Gln Pro Glu Val Lys Val Arg Pro Ile Lys Gln Val Ala Pro Gly 195 200 205 Leu Gly Val Gln Thr Val Asp Ile Lys Ile Pro Thr Glu Ser Met Glu 210 215 220 Val Gln Thr Glu Pro Ala Lys Pro Ala Ala Thr Ser Ile Glu Val Gln 225 230 235 240 Thr Asp Pro Trp Met Pro Ala Pro Ile Ala Thr Thr Ala Ser Thr Ala 245 250 255 Arg Arg Pro Arg Arg Lys Tyr Gly Pro Ala Ser Leu Leu Met Pro Asn 260 265 270 Tyr Ala Leu His Pro Ser Ile Ile Pro Thr Pro Gly Tyr Arg Gly Thr 275 280 285 Arg Tyr Tyr Arg Ser Arg Ser Thr Thr Ser Arg Arg Arg Lys Thr Pro 290 295 300 Ala Ser Arg Ser Arg Arg Arg Arg Arg Arg Thr Thr Ser Lys Leu Thr 305 310 315 320 Pro Ala Ala Leu Val Arg Arg Val Tyr Arg Asp Gly Arg Ala Glu Pro 325 330 335 Leu Met Leu Pro Arg Ala Arg Tyr His Pro Ser Ile Thr Thr 340 345 350 <210> 47 <211> 75 <212> PRT <213> Simian adenovirus 27 <400> 47 Met Ala Leu Thr Cys Arg Leu Arg Val Pro Ile Thr Gly Tyr Arg Gly 1 5 10 15 Arg Asn Ser Arg Arg Arg Arg Met Leu Gly Ser Gly Met Arg Arg His 20 25 30 Arg Arg Arg Arg Ala Thr Ser Arg Arg Leu Gly Gly Gly Phe Leu Thr 35 40 45 Ala Leu Ile Pro Ile Ile Ala Ala Ala Ile Gly Ala Val Pro Gly Ile 50 55 60 Ala Ser Val Ala Val Gln Ala Ser Gln Arg His 65 70 75 <210> 48 <211> 250 <212> PRT <213> Simian adenovirus 27 <400> 48 Met Glu Asp Ile Asn Phe Ser Ser Leu Ala Pro Arg His Gly Thr Arg 1 5 10 15 Pro Tyr Met Gly Thr Trp Ser Asp Ile Gly Thr Ser Gln Leu Asn Gly 20 25 30 Gly Ala Phe Asn Trp Ser Ser Ile Trp Ser Gly Leu Lys Asn Phe Gly 35 40 45 Ser Ala Ile Lys Thr Tyr Gly Asn Lys Ala Trp Asn Ser Ser Thr Gly 50 55 60 Gln Ala Leu Arg Asn Lys Leu Lys Glu Gln Asn Phe Gln Gln Lys Val 65 70 75 80 Val Asp Gly Ile Ala Ser Gly Ile Asn Gly Val Val Asp Leu Ala Asn 85 90 95 Gln Ala Val Gln Lys Gln Ile Asn Ser Arg Leu Asp Pro Pro Pro Ala 100 105 110 Ala Pro Gly Glu Met Glu Val Glu Glu Glu Leu Pro Pro Leu Glu Lys 115 120 125 Arg Gly Asp Lys Arg Pro Arg Pro Asp Met Glu Glu Thr Leu Val Thr 130 135 140 Arg Gly Asp Glu Pro Pro Pro Tyr Glu Glu Ala Ile Lys Leu Gly Met 145 150 155 160 Pro Thr Thr Lys Pro Ile Ala Pro Met Ala Thr Gly Val Met Lys Pro 165 170 175 Ser Gln Ser His Arg Pro Ala Thr Leu Asp Leu Pro Pro Ala Pro Ala 180 185 190 Ala Ala Ala Pro Ala Pro Lys Pro Val Ala Thr Pro Lys Pro Thr Thr 195 200 205 Val Gln Pro Val Ala Val Ala Arg Pro Arg Pro Gly Gly Thr Pro Arg 210 215 220 Pro Asn Ala Asn Trp Gln Ser Thr Leu Asn Ser Ile Val Gly Leu Gly 225 230 235 240 Val Gln Ser Val Lys Arg Arg Arg Cys Tyr 245 250 <210> 49 <211> 956 <212> PRT <213> Simian adenovirus 27 <400> 49 Met Ala Thr Pro Ser Met Leu Pro Gln Trp Ala Tyr Met His Ile Ala 1 5 10 15 Gly Gln Asp Ala Ser Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala 20 25 30 Arg Ala Thr Asp Thr Tyr Phe Asn Leu Gly Asn Lys Phe Arg Asn Pro 35 40 45 Thr Val Ala Pro Thr His Asp Val Thr Thr Asp Arg Ser Gln Arg Leu 50 55 60 Met Leu Arg Phe Val Pro Val Asp Arg Glu Asp Asn Thr Tyr Ser Tyr 65 70 75 80 Lys Val Arg Tyr Thr Leu Ala Val Gly Asp Asn Arg Val Leu Asp Met 85 90 95 Ala Ser Thr Phe Phe Asp Ile Arg Gly Val Leu Asp Arg Gly Pro Ser 100 105 110 Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser Leu Ala Pro Lys Gly 115 120 125 Ala Pro Asn Thr Cys Gln Trp Lys Tyr Lys Thr Leu Ala Asn Thr Glu 130 135 140 Thr Glu Glu Glu Glu Glu Glu Asp Glu Gln Ala Asp Glu Gln Glu Tyr 145 150 155 160 Val Glu Lys Thr Ser Thr Phe Gly Asn Ala Pro Val Lys Gly Leu Asp 165 170 175 Ile Asp Ala Asp Gly Leu Gln Ile Gly Val Asp Ile Gln Asp Glu Thr 180 185 190 Lys Pro Val Tyr Ala Asn Lys Leu Tyr Glu Pro Glu Pro Gln Val Gly 195 200 205 Asp Gly Gln Trp His Asp Thr Thr Ala Ile Thr Glu Gln Tyr Gly Gly 210 215 220 Arg Ala Leu Lys Pro Asp Thr Lys Met Lys Pro Cys Tyr Gly Ser Phe 225 230 235 240 Ala Arg Pro Thr Asn Glu Lys Gly Gly Gln Ser Lys Thr Arg Ser Val 245 250 255 Thr Lys Asp Asp Lys Val Val Glu Glu Pro Asp Ile Asp Met Ala Phe 260 265 270 Phe Asp Gly Arg Asp Ala Lys Lys Pro Asp Pro Glu Ile Val Leu Tyr 275 280 285 Thr Glu Asn Val Asn Leu Glu Thr Pro Asp Thr His Ile Val Tyr Lys 290 295 300 Ala Gly Thr Asp Asp Ser Ser Ser Ser Ile Asn Leu Gly Gln Gln Ser 305 310 315 320 Met Pro Asn Arg Pro Asn Tyr Ile Gly Phe Arg Asp Asn Phe Ile Gly 325 330 335 Leu Met Tyr Tyr Asn Ser Thr Gly Asn Met Gly Val Leu Ala Gly Gln 340 345 350 Ala Ser Gln Leu Asn Ala Val Val Asp Leu Gln Asp Arg Asn Thr Glu 355 360 365 Leu Ser Tyr Gln Leu Leu Leu Asp Ser Leu Gly Asp Arg Thr Arg Tyr 370 375 380 Phe Ser Met Trp Asn Gln Ala Val Asp Ser Tyr Asp Pro Asp Val Arg 385 390 395 400 Ile Ile Glu Asn His Gly Val Glu Asp Glu Leu Pro Asn Tyr Cys Phe 405 410 415 Pro Leu Asp Gly Val Gly Arg Thr Asp Thr Tyr Lys Gly Val Val Val 420 425 430 Asp Gln Ala Ala Ala Ala Gly Thr Ala Thr Thr Trp Lys Asp Asp Thr 435 440 445 Thr Ala Asn Glu Tyr Asn Glu Ile Ala Lys Gly Asn Asn Leu Ala Met 450 455 460 Glu Ile Asn Leu Gln Ala Asn Leu Trp Arg Ser Phe Leu Tyr Ser Asn 465 470 475 480 Val Ala Leu Tyr Leu Pro Asp Ala Tyr Lys Tyr Thr Pro Ala Asn Val 485 490 495 Thr Leu Pro Thr Asn Thr Asn Thr Tyr Glu Tyr Met Asn Gly Arg Val 500 505 510 Val Ser Pro Ser Leu Val Asp Ala Tyr Val Asn Ile Gly Ala Arg Trp 515 520 525 Ser Leu Asp Pro Met Asp Asn Val Asn Pro Phe Asn His His Arg Asn 530 535 540 Ala Gly Leu Arg Tyr Arg Ser Met Leu Leu Gly Asn Gly Arg Tyr Val 545 550 555 560 Pro Phe His Ile Gln Val Pro Gln Lys Phe Phe Ala Val Lys Asn Leu 565 570 575 Leu Leu Leu Pro Gly Ser Tyr Thr Tyr Glu Trp Asn Phe Arg Lys Asp 580 585 590 Val Asn Met Val Leu Gln Ser Ser Leu Gly Asn Asp Leu Arg Thr Asp 595 600 605 Gly Ala Thr Ile Ser Phe Thr Ser Ile Asn Leu Tyr Ala Thr Phe Phe 610 615 620 Pro Met Ala His Asn Thr Ala Ser Thr Leu Glu Ala Met Leu Arg Asn 625 630 635 640 Asp Thr Asn Asp Gln Ser Phe Asn Asp Tyr Leu Ser Ala Ala Asn Met 645 650 655 Leu Tyr Pro Ile Pro Ala Asn Ala Thr Asn Ile Pro Ile Ser Ile Pro 660 665 670 Ser Arg Asn Trp Ala Ala Phe Arg Gly Trp Ser Phe Thr Arg Leu Lys 675 680 685 Thr Lys Glu Thr Pro Ser Leu Gly Ser Gly Phe Asp Pro Tyr Phe Val 690 695 700 Tyr Ser Gly Ser Ile Pro Tyr Leu Asp Gly Thr Phe Tyr Leu Asn His 705 710 715 720 Thr Phe Lys Lys Val Ser Ile Met Phe Asp Ser Ser Val Ser Trp Pro 725 730 735 Gly Asn Asp Arg Leu Leu Thr Pro Asn Glu Phe Glu Ile Lys Arg Thr 740 745 750 Val Asp Gly Glu Gly Tyr Asn Val Ala Gln Cys Asn Met Thr Lys Asp 755 760 765 Trp Phe Leu Val Gln Met Leu Ala Asn Tyr Asn Ile Gly Tyr Gln Gly 770 775 780 Phe Tyr Ile Pro Glu Gly Tyr Lys Asp Arg Met Tyr Ser Phe Phe Arg 785 790 795 800 Asn Phe Gln Pro Met Ser Arg Gln Val Val Asp Glu Ile Asn Tyr Thr 805 810 815 Asp Tyr Lys Ala Val Lys Leu Pro Phe Gln His Asn Asn Ser Gly Phe 820 825 830 Val Gly Tyr Leu Ala Pro Thr Ile Arg Gln Gly Gln Ala Tyr Pro Ala 835 840 845 Asn Tyr Pro Tyr Pro Leu Ile Gly Ser Thr Ala Val Lys Ser Val Thr 850 855 860 Gln Lys Lys Phe Leu Cys Asp Arg Thr Met Trp Arg Ile Pro Phe Ser 865 870 875 880 Ser Asn Phe Met Ser Met Gly Ala Leu Thr Asp Leu Gly Gln Asn Met 885 890 895 Leu Tyr Ala Asn Ser Ala His Ala Leu Asp Met Thr Phe Glu Val Asp 900 905 910 Pro Met Asp Glu Pro Thr Leu Leu Tyr Leu Leu Phe Glu Val Phe Asp 915 920 925 Val Val Arg Val His Gln Pro His Arg Gly Val Ile Glu Ala Val Tyr 930 935 940 Leu Arg Thr Pro Phe Ser Ala Gly Asn Ala Thr Thr 945 950 955 <210> 50 <211> 209 <212> PRT <213> Simian adenovirus 27 <400> 50 Met Ala Cys Gly Ser Gly Asn Gly Ser Ser Glu Gln Glu Leu Arg Ala 1 5 10 15 Ile Ala Arg Asp Leu Gly Cys Gly Pro Tyr Phe Leu Gly Thr Phe Asp 20 25 30 Lys Arg Phe Pro Gly Phe Met Ala Pro Asp Lys Leu Ala Cys Ala Ile 35 40 45 Val Asn Thr Ala Gly Arg Glu Thr Gly Gly Glu His Trp Leu Ala Phe 50 55 60 Gly Trp Asn Pro Arg Ser Asn Thr Cys Tyr Leu Phe Asp Pro Phe Gly 65 70 75 80 Phe Ser Asp Glu Arg Leu Lys Gln Ile Tyr Gln Phe Glu Tyr Glu Gly 85 90 95 Leu Leu Arg Arg Ser Ala Leu Ala Thr Lys Asp Arg Cys Ile Thr Leu 100 105 110 Glu Lys Ser Thr Gln Thr Val Gln Gly Pro Arg Ser Ala Ala Cys Gly 115 120 125 Leu Phe Cys Cys Met Phe Leu His Ala Phe Val His Trp Pro Asp Arg 130 135 140 Pro Met Asp Gly Asn Pro Thr Met Lys Leu Leu Thr Gly Val Pro Asn 145 150 155 160 Ser Met Leu Gln Ser Pro Gln Val Gln Pro Thr Leu Arg His Asn Gln 165 170 175 Glu Ala Leu Tyr Arg Phe Leu Asn Thr His Ser Ser Tyr Phe Arg Ser 180 185 190 His Arg Ala Arg Ile Glu Lys Ala Thr Ala Phe Asp Arg Met Ala Met 195 200 205 Gln <210> 51 <211> 828 <212> PRT <213> Simian adenovirus 27 <400> 51 Met Glu Thr Gln Pro Ser Leu Pro Thr Pro Leu Gln Ala Pro Ser His 1 5 10 15 Leu Ala Ser Ser Asp Glu Glu Glu Glu Gln Ser Leu Thr Ala Pro Pro 20 25 30 Pro Ser Pro Ala Thr Thr Thr Ser Thr Leu Glu Asp Glu Glu Val Asp 35 40 45 Ala Pro Gln Glu Ile Arg Thr Gln Asp Met Glu Asp Glu Lys Ala Glu 50 55 60 Glu Ile Glu Ala Asp Ile Glu Gln Asp Pro Gly Tyr Val Thr Pro Ala 65 70 75 80 Glu His Glu Glu Glu Leu Arg Arg Phe Leu Glu Lys Asp Asp Asp Asn 85 90 95 Arg Pro Glu Gln Gln Ala Asp Gly Asp Gln Gln Asn Val Gly Leu Gly 100 105 110 Asp His Val Val Asp Tyr Leu Thr Gly Leu Gly Gly Glu Asp Val Leu 115 120 125 Leu Lys His Leu Ala Arg Gln Ser Ile Ile Ile Lys Asp Ala Leu Leu 130 135 140 Asp Arg Ser Glu Val Pro Ile Ser Val Glu Glu Leu Ser Arg Ala Tyr 145 150 155 160 Glu Leu Asn Leu Phe Ser Pro Arg Val Pro Pro Lys Arg Gln Pro Asn 165 170 175 Gly Thr Cys Glu Pro Asn Pro Arg Leu Asn Phe Tyr Pro Ala Phe Thr 180 185 190 Val Pro Glu Val Leu Ala Thr Tyr His Ile Phe Phe Lys Asn Gln Lys 195 200 205 Ile Pro Ile Ser Cys Arg Ala Asn Arg Thr Arg Ala Asp Ala Leu Leu 210 215 220 Asn Leu Gly Pro Gly Ala Cys Leu Pro Asp Ile Thr Ser Leu Glu Glu 225 230 235 240 Val Pro Lys Ile Phe Glu Gly Leu Gly Ser Asp Glu Thr Arg Ala Ala 245 250 255 Asn Ala Leu Gln Gln Gly Glu Asn Gly Ile Asp Glu His His Ser Ala 260 265 270 Leu Val Glu Leu Glu Gly Asp Asn Ala Arg Leu Ala Val Leu Lys Arg 275 280 285 Ser Ile Glu Val Thr His Phe Ala Tyr Pro Ala Val Asn Leu Pro Pro 290 295 300 Lys Val Met Ser Ala Val Met Asp Gln Ile Leu Ile Lys Arg Ala Ser 305 310 315 320 Pro Leu Ser Glu Asn Met Gln Asp Pro Asp Ala Ser Asp Glu Gly Lys 325 330 335 Pro Val Val Ser Asp Glu Gln Leu Ser Arg Trp Leu Gly Thr Asn Ser 340 345 350 Pro Arg Asp Leu Glu Glu Arg Arg Lys Leu Met Met Ala Val Val Leu 355 360 365 Val Thr Val Glu Met Glu Cys Leu Arg Arg Phe Phe Thr Asp Pro Glu 370 375 380 Thr Leu Arg Lys Leu Glu Glu Asn Leu His Tyr Thr Phe Arg His Gly 385 390 395 400 Phe Val Arg Gln Ala Cys Lys Ile Ser Asn Val Glu Leu Thr Asn Leu 405 410 415 Val Ser Tyr Met Gly Ile Leu His Glu Asn Arg Leu Gly Gln Ser Val 420 425 430 Leu His Thr Thr Leu Lys Gly Glu Ala Arg Arg Asp Tyr Ile Arg Asp 435 440 445 Thr Val Tyr Leu Tyr Leu Cys His Thr Trp Gln Thr Gly Met Gly Val 450 455 460 Trp Gln Gln Cys Leu Glu Glu Gln Asn Leu Lys Glu Leu Asp Lys Leu 465 470 475 480 Leu Gln Arg Ser Leu Lys Thr Leu Trp Thr Gly Phe Asp Glu Arg Thr 485 490 495 Val Ala Ser Asp Leu Ala Asp Leu Ile Phe Pro Glu Arg Leu Arg Thr 500 505 510 Thr Leu Arg Asn Gly Leu Pro Asp Phe Met Asn Gln Ser Met Ile Asn 515 520 525 Asn Phe Arg Ser Phe Ile Leu Glu Arg Ser Gly Ile Leu Pro Ala Thr 530 535 540 Cys Cys Ala Leu Pro Ser Asp Phe Val Pro Leu Thr Tyr Arg Glu Cys 545 550 555 560 Pro Pro Pro Leu Trp Ser His Cys Tyr Leu Phe Arg Leu Ala Asn Tyr 565 570 575 Leu Ser Tyr His Ser Asp Val Ile Glu Asp Val Ser Gly Asp Gly Leu 580 585 590 Leu Glu Cys His Cys Arg Cys Asn Leu Cys Ser Pro His Arg Ser Leu 595 600 605 Val Cys Asn Pro Gln Leu Leu Ser Glu Thr Gln Ile Ile Gly Thr Phe 610 615 620 Glu Leu Gln Gly Pro Ser Ser Glu Gly Glu Gly Ser Ser Pro Gly Gln 625 630 635 640 Ser Leu Lys Leu Thr Pro Gly Leu Trp Thr Ser Ala Tyr Leu Arg Lys 645 650 655 Phe Ser Pro Glu Asp Tyr His Pro Tyr Glu Ile Arg Phe Tyr Glu Asp 660 665 670 Gln Ser Gln Pro Pro Lys Ala Glu Leu Ser Ala Cys Val Ile Thr Gln 675 680 685 Gly Ala Ile Leu Ala Gln Leu Gln Ala Ile Gln Lys Ser Arg Gln Glu 690 695 700 Phe Leu Leu Lys Lys Gly Asn Gly Val Tyr Leu Asp Pro Gln Thr Gly 705 710 715 720 Glu Glu Leu Asn Thr Arg Phe Ser Gln Asp Val Ser Ala Pro Arg Lys 725 730 735 Gln Glu Val Glu Ser Ala Ala Ala Ala Pro Arg Gly Tyr Gly Gly Arg 740 745 750 Leu Gly Gln Ser Asp Arg Gly Asp Gly Arg Leu Gly Gln Pro Gly Arg 755 760 765 Gly Gly Gly Gly Gln Pro Gly Gly Arg Gln Phe Gly Gly Gly Arg Arg 770 775 780 Gly Gly Arg Gly Gly Gly Arg Ser Asn Arg Arg Gln Thr Val Val Leu 785 790 795 800 Gly Gly Gly Asp Lys Gln Gly His Arg Gln His His Ser Tyr His Leu 805 810 815 Arg Ser Gly Ser Gly Gly Pro Ala Pro Ser Gln Gln 820 825 <210> 52 <211> 227 <212> PRT <213> Simian adenovirus 27 <400> 52 Met Ser Lys Glu Ile Pro Thr Pro Tyr Met Trp Ser Tyr Gln Pro Gln 1 5 10 15 Met Gly Leu Ala Ala Gly Ala Ser Gln Asp Tyr Ser Thr Arg Met Asn 20 25 30 Trp Leu Ser Ala Gly Pro Ser Met Ile Ser Arg Val Asn Asp Ile Arg 35 40 45 Ala Tyr Arg Asn Gln Leu Leu Leu Glu Gln Ser Ala Leu Thr Ala Thr 50 55 60 Pro Arg Gln His Leu Asn Pro Arg Asn Trp Pro Ala Ala Leu Val Tyr 65 70 75 80 Gln Glu Thr Pro Ala Pro Thr Thr Val Leu Leu Pro Arg Asp Ala Gln 85 90 95 Ala Glu Val Gln Met Thr Asn Ala Gly Val Gln Leu Ala Gly Gly Ser 100 105 110 Ala Leu Cys Arg His Arg Pro Gln Gln Ser Ile Lys Arg Leu Val Ile 115 120 125 Arg Gly Arg Gly Ile Gln Leu Asn Asp Glu Ser Val Ser Ser Ser Leu 130 135 140 Gly Leu Arg Pro Asp Gly Val Phe Gln Ile Ala Gly Cys Gly Arg Ser 145 150 155 160 Ser Phe Thr Pro Arg Gln Ala Val Leu Thr Leu Glu Ser Ser Ser Ser 165 170 175 Gln Pro Arg Ser Gly Gly Ile Gly Thr Leu Gln Phe Val Glu Glu Phe 180 185 190 Thr Pro Ser Val Tyr Phe Asn Pro Phe Ser Gly Ser Pro Gly His Tyr 195 200 205 Pro Asp Glu Phe Ile Pro Asn Phe Asp Ala Ile Ser Glu Ser Val Asp 210 215 220 Gly Tyr Asp 225 <210> 53 <211> 106 <212> PRT <213> Simian adenovirus 27 <400> 53 Met Ser Asn Gly Gly Ala Ala Glu Leu Ala Arg Leu Arg His Leu Asp 1 5 10 15 His Cys Arg Arg Phe Arg Cys Phe Ala Arg Glu Leu Thr Glu Phe Ile 20 25 30 Tyr Phe Glu Ile Pro Glu Glu His Pro Gln Gly Pro Ala His Gly Val 35 40 45 Arg Ile Thr Ile Glu Gly Gly Ile Asp Ser Arg Leu His Arg Ile Phe 50 55 60 Cys Gln Arg Pro Val Leu Ile Glu Arg Asp Gln Gly Asn Thr Thr Val 65 70 75 80 Ser Ile Tyr Cys Ile Cys Asn His Pro Gly Leu His Glu Ser Leu Cys 85 90 95 Cys Leu Ile Cys Ala Glu Phe Asn Lys Asn 100 105 <210> 54 <211> 172 <212> PRT <213> Simian adenovirus 27 <400> 54 Met Gly Ala Leu Leu Val Ala Leu Ala Leu Leu Ser Leu Leu Asp Leu 1 5 10 15 Gly Ser Thr Met Leu Val Gln Pro Val Leu Phe Asp Pro Cys Leu Asn 20 25 30 Phe Asp Pro Asp Asn Cys Thr Leu Thr Phe Ala Pro Glu Ala Gly Arg 35 40 45 Cys Gly Val Leu Ile Arg Cys Gly Arg Glu Cys Ser Pro Ile Glu Ile 50 55 60 His His Asn Asn Lys Ile Trp Asn Asn Thr Leu Phe Thr Thr Trp Gln 65 70 75 80 Pro Gly Asp Pro Glu Trp Tyr Thr Val Ser Val Arg Gly Pro Asp Gly 85 90 95 Ser Ile Arg Thr Ala Asn Asn Thr Phe Ile Phe Ala Glu Met Cys Asp 100 105 110 Leu Thr Met Phe Met Ser Lys Gln Tyr Asn Leu Trp Pro Pro Ser Lys 115 120 125 Glu Asn Ile Val Ala Phe Ser Ile Ala Tyr Phe Leu Cys Thr Cys Leu 130 135 140 Ile Thr Ala Ile Leu Cys Ile Cys Ile His Leu Leu Ile Cys His Arg 145 150 155 160 His Arg Asn Ser Asn Glu Glu Lys Glu Lys Met Pro 165 170 <210> 55 <211> 177 <212> PRT <213> Simian adenovirus 27 <400> 55 Met Ala Ser Val Ile Ala Leu Ile Ile Val Ser Ile Leu Thr Ala Ala 1 5 10 15 Gln Gly Gln Thr Ile Val Tyr Ile Thr Leu Gly His Asn His Thr Leu 20 25 30 Ile Gly Pro Gln Ile Ser Ser Gln Val Ile Trp Thr Lys Leu Gly Ser 35 40 45 Val Asp Tyr Phe Asp Ile Ile Cys Asn Arg Thr Lys Pro Ile Phe Val 50 55 60 Thr Cys Asn Lys Gln Asn Leu Thr Leu Ile Asn Val Ser Glu Ile Tyr 65 70 75 80 Ser Gly Tyr Tyr Tyr Gly Tyr Asp Arg His Ser Ser Glu Tyr Lys Asn 85 90 95 Tyr Leu Val Arg Ile Thr Gln Pro Lys Thr Thr Lys Met Pro Asn Lys 100 105 110 Ala Lys Ile Gln Met Val Ser Ala Leu Glu His Leu Thr Tyr Pro Thr 115 120 125 Thr Pro Asp Glu Arg Asn Ile Pro Asn Ser Met Ile Ala Ile Ile Ala 130 135 140 Ala Val Ala Val Gly Met Ala Leu Ile Ile Ile Cys Met Phe Leu Tyr 145 150 155 160 Ala Cys Tyr Cys Arg Lys Phe His His Lys Gln Asp Ser Leu Leu Asn 165 170 175 Phe <210> 56 <211> 189 <212> PRT <213> Simian adenovirus 27 <400> 56 Met Val Ser Thr Thr Ala Phe Phe Val Ile Ser Ser Leu Ala Ala Val 1 5 10 15 Thr Tyr Gly Arg Ser His Leu Thr Val Thr Val Gly Ser Thr Cys Thr 20 25 30 Leu Gln Gly Pro Gln Glu Gly His Val Ser Trp Trp Arg Ile Tyr Asp 35 40 45 Ser Gly Trp Phe Ile Arg Pro Cys Asp Gln Pro Gly Asn Lys Phe Phe 50 55 60 Cys Asn Gly Arg Asp Leu Thr Ile Ile Asn Ile Thr Val Asn Asp Gln 65 70 75 80 Gly Phe Tyr Tyr Gly Thr Asn Tyr Lys Asn Asn Leu Asp Tyr Asn Ile 85 90 95 Ile Val Val Pro Ala Thr Thr Pro Ala Pro Arg Lys Thr Thr Phe Phe 100 105 110 Ser Ser Ser Ala Ser Ile Ser Lys Thr Ala Ser Ala Ser Phe Lys Lys 115 120 125 Phe Ala Leu Arg Asn Ser Thr Thr Ser Ser Thr Ser Asn Asn Thr Met 130 135 140 Ser Lys Ser Val Ile Gly Ile Ala Ala Ala Ala Ile Val Gly Leu Met 145 150 155 160 Ile Ile Ile Leu Cys Ile Ile Tyr Tyr Ala Cys Cys Tyr Arg Lys Gln 165 170 175 His Glu Gln Lys Thr Asp Pro Leu Leu Asn Phe Asp Ile 180 185 <210> 57 <211> 101 <212> PRT <213> Simian adenovirus 27 <400> 57 Met Lys Lys Leu Ser Ile Leu Ala Phe Ile Leu Phe Gln Thr Phe Thr 1 5 10 15 Asn Val Gln Thr Thr Leu Ser His Gly Ile Glu Asn His Thr Thr Ser 20 25 30 Tyr Glu Leu Thr Asn Ile Thr Thr His His Pro Lys Tyr Ala Met Gln 35 40 45 Leu Glu Ile Thr Met Leu Ile Val Val Gly Ile Leu Ile Leu Ala Ile 50 55 60 Ile Phe Tyr Phe Thr Leu Cys Arg Gln Ile Pro Asn Ile His Lys Asn 65 70 75 80 Ser Lys Arg Arg Pro Ile Tyr Cys Pro Val Ile Ser Arg Pro His Met 85 90 95 Thr Leu Asn Glu Ile 100 <210> 58 <211> 145 <212> PRT <213> Simian adenovirus 27 <400> 58 Met Leu Arg His Phe Ser Asp Leu Phe Lys Thr Met Gln Ala Ile Leu 1 5 10 15 Pro Val Ile Leu Leu Leu Leu Leu Pro Cys Asp Ala Leu Thr Pro Val 20 25 30 Ala Asn Arg Thr Pro Pro Glu Gln Leu Arg Lys Cys Lys Phe Gln Gln 35 40 45 Pro Trp Thr Phe Leu Asp Cys Tyr Arg Glu Lys Ser Asp Phe Pro Thr 50 55 60 Tyr Trp Ile Met Ile Ile Gly Ile Val Asn Leu Val Ser Cys Thr Leu 65 70 75 80 Phe Ser Phe Leu Val Tyr His Phe Phe Asp Phe Gly Trp Asn Ala Pro 85 90 95 Asn Ala Leu Thr Tyr Pro Gln Glu Pro Glu Glu His Ile Pro Leu Gln 100 105 110 Asn Met Gln Gln Pro Ile Ala Ile Ile Asp Tyr Asp Asn Glu Pro Gln 115 120 125 Pro Ser Leu Leu Pro Ala Ile Ser Tyr Phe Asn Leu Thr Gly Gly Asp 130 135 140 Asp 145 <210> 59 <211> 325 <212> PRT <213> Simian adenovirus 27 <400> 59 Met Ala Lys Arg Val Arg Leu Ser Ser Ser Phe Asn Pro Val Tyr Pro 1 5 10 15 Tyr Glu Asp Glu Ser Ser Ser Gln His Pro Phe Ile Asn Pro Gly Phe 20 25 30 Ile Ser Ser Asn Gly Phe Thr Gln Ser Pro Asp Gly Val Leu Thr Leu 35 40 45 Lys Cys Val Ala Pro Leu Thr Thr Thr Ser Gly Ala Leu Asp Ile Lys 50 55 60 Val Gly Gly Gly Leu Lys Val Asp Ser Thr Asp Gly Ser Leu Glu Glu 65 70 75 80 Asp Met Gly Ile Ala Ala Pro Leu Thr Lys Val Asn His Ser Val Gly 85 90 95 Leu Ala Leu Gly Asp Gly Leu Glu Thr Lys Glu Asn Lys Leu Tyr Val 100 105 110 Lys Leu Gly Glu Gly Leu Lys Phe Asn Ser Gly Ser Ile Asn Ile Asp 115 120 125 His Asp Ile Asn Thr Leu Trp Thr Gly Val Asn Pro Ser Ala Asn Cys 130 135 140 Ile Ile Thr Glu Asp Gly Glu Ala Asn Asp Ser Lys Leu Thr Leu Ile 145 150 155 160 Leu Val Lys Thr Gly Gly Leu Val Asn Ala Tyr Val Ser Leu Met Gly 165 170 175 Asp Ser Glu Ala Val Asn Lys Leu Thr Thr Asp Lys Ser Ala Gln Ile 180 185 190 Thr Val Asp Ile Tyr Phe Asp Asn Glu Gly Lys Val Leu Thr Glu Leu 195 200 205 Ser Ala Leu Lys Thr Gly Leu Lys His Lys Phe Gly Gln Asn Met Ala 210 215 220 Ser Asp Glu Ala Gln Asn Cys Lys Gly Phe Met Pro Ser Leu Thr Ala 225 230 235 240 Tyr Pro Phe Arg Asn Pro Thr Lys Pro Thr Lys Gly Arg Glu Asp Tyr 245 250 255 Ile Tyr Gly Ile Thr Tyr Tyr Gln Ala Thr Asp Gly Thr Leu Tyr Glu 260 265 270 Leu Lys Thr Thr Val Thr Leu Asn Tyr Ser Val Ile Ser Ser Leu Cys 275 280 285 Ala Tyr Ala Met His Ile Ser Trp Ser Trp Asp Ser Val Thr Glu Pro 290 295 300 Glu Thr Thr Pro Thr Thr Leu Ile Thr Ser Pro Phe Ser Phe Ser Tyr 305 310 315 320 Ile Arg Glu Asp Asp 325 <210> 60 <211> 550 <212> DNA <213> Simian adenovirus 27 <220> <221> CDS <222> (1)..(543) <223> label=Elb\19K <400> 60 atg gag gtt tgg gct atc ttg gaa gat ctc aga cag act agg caa ctg 48 Met Glu Val Trp Ala Ile Leu Glu Asp Leu Arg Gln Thr Arg Gln Leu 1 5 10 15 cta gaa aac gcc tcg gac gga gtc tct agt ctt tgg aga ttc tgg ttc 96 Leu Glu Asn Ala Ser Asp Gly Val Ser Ser Leu Trp Arg Phe Trp Phe 20 25 30 ggt ggt gat cta gct agg cta gtc ttc agg gta aaa cgg gag tat agt 144 Gly Gly Asp Leu Ala Arg Leu Val Phe Arg Val Lys Arg Glu Tyr Ser 35 40 45 gaa gaa ttt gaa aag tta ttg gaa gac agt cca gga ctt ttt gaa gct 192 Glu Glu Phe Glu Lys Leu Leu Glu Asp Ser Pro Gly Leu Phe Glu Ala 50 55 60 ctt aac ttg ggc cac cag gct cat ttt aag gag aag gtt tta tca gtt 240 Leu Asn Leu Gly His Gln Ala His Phe Lys Glu Lys Val Leu Ser Val 65 70 75 80 tta gat ttt tct acc cct ggt aga act gct gca gct gta gcc ttc ctt 288 Leu Asp Phe Ser Thr Pro Gly Arg Thr Ala Ala Ala Val Ala Phe Leu 85 90 95 act ttt ata ttg gat aaa tgg atc cca caa acc cac ttc agc aag gga 336 Thr Phe Ile Leu Asp Lys Trp Ile Pro Gln Thr His Phe Ser Lys Gly 100 105 110 tac gtt ttg gat ttc ata gca gca gct ttg tgg aga aca tgg aag gct 384 Tyr Val Leu Asp Phe Ile Ala Ala Ala Leu Trp Arg Thr Trp Lys Ala 115 120 125 cgc agg ctg agg ata atc tta gat tac tgg cca gtg cag cct ctg ggc 432 Arg Arg Leu Arg Ile Ile Leu Asp Tyr Trp Pro Val Gln Pro Leu Gly 130 135 140 gta gcg gcg atc ctg aga cac cca ccg gcc atg cca gcg gtt ctg gag 480 Val Ala Ala Ile Leu Arg His Pro Pro Ala Met Pro Ala Val Leu Glu 145 150 155 160 gag gag cag cag gag gac aac ccg aga gcc ggc ctg gac cct ccg gtg 528 Glu Glu Gln Gln Glu Asp Asn Pro Arg Ala Gly Leu Asp Pro Pro Val 165 170 175 gag gag gcg gag gag tagctga 550 Glu Glu Ala Glu Glu 180 <210> 61 <211> 181 <212> PRT <213> Simian adenovirus 27 <400> 61 Met Glu Val Trp Ala Ile Leu Glu Asp Leu Arg Gln Thr Arg Gln Leu 1 5 10 15 Leu Glu Asn Ala Ser Asp Gly Val Ser Ser Leu Trp Arg Phe Trp Phe 20 25 30 Gly Gly Asp Leu Ala Arg Leu Val Phe Arg Val Lys Arg Glu Tyr Ser 35 40 45 Glu Glu Phe Glu Lys Leu Leu Glu Asp Ser Pro Gly Leu Phe Glu Ala 50 55 60 Leu Asn Leu Gly His Gln Ala His Phe Lys Glu Lys Val Leu Ser Val 65 70 75 80 Leu Asp Phe Ser Thr Pro Gly Arg Thr Ala Ala Ala Val Ala Phe Leu 85 90 95 Thr Phe Ile Leu Asp Lys Trp Ile Pro Gln Thr His Phe Ser Lys Gly 100 105 110 Tyr Val Leu Asp Phe Ile Ala Ala Ala Leu Trp Arg Thr Trp Lys Ala 115 120 125 Arg Arg Leu Arg Ile Ile Leu Asp Tyr Trp Pro Val Gln Pro Leu Gly 130 135 140 Val Ala Ala Ile Leu Arg His Pro Pro Ala Met Pro Ala Val Leu Glu 145 150 155 160 Glu Glu Gln Gln Glu Asp Asn Pro Arg Ala Gly Leu Asp Pro Pro Val 165 170 175 Glu Glu Ala Glu Glu 180 <210> 62 <211> 5470 <212> DNA <213> Simian adenovirus 27 <220> <221> CDS <222> (9)..(602) <223> label=22K <220> <221> CDS <222> (1908)..(2345) <223> label=E3\CR1-alpha <220> <221> CDS <222> (4376)..(4648) <223> label=E3\RID-alpha <220> <221> CDS <222> (5053)..(5457) <223> label=E3\14.7K <400> 62 ctctcagg atg tct cag cgc cga gga aac aag aag ttg aaa gtg cag ctg 50 Met Ser Gln Arg Arg Gly Asn Lys Lys Leu Lys Val Gln Leu 1 5 10 ccg ccc cca gag gat atg gag gaa gac tgg gac agt cag aca gag gag 98 Pro Pro Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Thr Glu Glu 15 20 25 30 atg gaa gat tgg gac agc cag gca gag gag gag gag gac agc ctg gag 146 Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Glu Glu Asp Ser Leu Glu 35 40 45 gaa gac agt ttg gag gag gaa gac gag gag gca gag gag gtg gaa gaa 194 Glu Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu 50 55 60 gca acc gcc gcc aaa cag ttg tcc tcg gcg gcg gag aca agc aag gcc 242 Ala Thr Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Ala 65 70 75 aca gac aac acc aca gct acc atc tcc gtt ccg ggt cgg ggg gtc cag 290 Thr Asp Asn Thr Thr Ala Thr Ile Ser Val Pro Gly Arg Gly Val Gln 80 85 90 cac cgt ccc aac agt aga tgg gat gag acc ggg cga ctc ccg aat gcg 338 His Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Leu Pro Asn Ala 95 100 105 110 acc acc gct tct aag act ggt aag aag gag cgg cag gga tac aag tcc 386 Thr Thr Ala Ser Lys Thr Gly Lys Lys Glu Arg Gln Gly Tyr Lys Ser 115 120 125 tgg cgg ggg cat aag aac gct atc ata tcc tgc ttg cat gaa tgc ggg 434 Trp Arg Gly His Lys Asn Ala Ile Ile Ser Cys Leu His Glu Cys Gly 130 135 140 ggc aac ata tcc ttc acc cgc cgc tac ctg ctc ttc cac cac ggg gtg 482 Gly Asn Ile Ser Phe Thr Arg Arg Tyr Leu Leu Phe His His Gly Val 145 150 155 aac ttc ccc cgc aat gtc ttg cat tac tac cgt cac ctc cac agc ccc 530 Asn Phe Pro Arg Asn Val Leu His Tyr Tyr Arg His Leu His Ser Pro 160 165 170 tac tac agc cag caa gcc tcg gca gaa aaa gac aac agc agc aag aac 578 Tyr Tyr Ser Gln Gln Ala Ser Ala Glu Lys Asp Asn Ser Ser Lys Asn 175 180 185 190 ctc cag cag aaa acc agc agc agt tagaacaccc acagcaggtg caacaggagg 632 Leu Gln Gln Lys Thr Ser Ser Ser 195 aggactgaga atcacagcga acgagccagc gcagacccga gagctgagaa accggatttt 692 tccaaccctc tatgccatct tccaacagag tcgggggcaa gagcaggaac tgaaagtaaa 752 aaaccgatct ttgcgctcgc tcacccgaag ttgtttgtat cacaagagcg aagaccaact 812 tcagcgcact ctcgaggacg ccgaggctct cttcaacaag tactgcgcgc tcactcttaa 872 agagtagccc gcgcccgcgc tagctcgaaa aaaggcggga attacgtcac ccattggcgc 932 ctgtcctttg ccctcgtcat gagtaaagaa attcccacgc cttacatgtg gagttatcaa 992 ccccaaatgg gactggcagc aggcgcctcc caggactact ccacccgtat gaattggctc 1052 agcgccggtc cctcgatgat ctcacgggtt aatgatatac gagcttatcg aaaccaatta 1112 ctcctagaac agtcagcact taccgccaca cccagacaac accttaatcc ccggaattgg 1172 cccgccgccc tggtgtacca ggaaaccccc gctcccacca ccgtcctact tcctcgagac 1232 gcccaggccg aagttcagat gactaacgca ggtgtacagc tggctggcgg ttccgccctg 1292 tgtcgtcacc ggcctcaaca gagtataaaa cgcctggtga tcagaggccg aggtatccag 1352 ctcaacgacg agtcggtgag ctcttcgctt ggtctacgac cagacggagt cttccaaatt 1412 gccggctgcg ggagatcttc cttcactcct cgtcaggctg tactgacttt ggagagttcg 1472 tcatcgcagc cccgctcggg tggcatcggg actctccaat ttgtggagga gtttactccc 1532 tctgtctact tcaacccctt ctccggctct cctgggcatt atccggacga gttcatacca 1592 aacttcgacg caatcagcga gtcagtggat ggctatgatt gatgtctaat ggtggcgcgg 1652 ctgagctagc tcgactgcga catctagacc actgccgccg ctttcgctgc tttgcccgag 1712 aactcaccga gttcatctac ttcgaaatac ccgaggagca ccctcaagga ccggcccacg 1772 gagtgcgtat taccatcgaa ggggggatag actctcgcct gcatcggatc ttctgccagc 1832 gacccgtgct aatcgagcgc gaccagggaa acaccacagt ctccatctac tgcatctgta 1892 accaccccgg attgc atg aaa gcc ttt gct gtc tta ttt gtg ctg agt tta 1943 Met Lys Ala Phe Ala Val Leu Phe Val Leu Ser Leu 200 205 210 ata aaa act gag tta aga ctc tcc tac gga cta cca att ctt caa ctc 1991 Ile Lys Thr Glu Leu Arg Leu Ser Tyr Gly Leu Pro Ile Leu Gln Leu 215 220 225 gga ctt tat aac aat cag acc ctc cgt tca agt cag aag acc cca acc 2039 Gly Leu Tyr Asn Asn Gln Thr Leu Arg Ser Ser Gln Lys Thr Pro Thr 230 235 240 ctt cct ctg atc cag gaa tct aat tct acc tcc cca gca cca cac ttt 2087 Leu Pro Leu Ile Gln Glu Ser Asn Ser Thr Ser Pro Ala Pro His Phe 245 250 255 act agc ctt ccc gaa act aac aac ctc gga gct caa ctg cac cac ttt 2135 Thr Ser Leu Pro Glu Thr Asn Asn Leu Gly Ala Gln Leu His His Phe 260 265 270 tcc aga agc ctt ctc tct gcc aat act acc act ccc aga acc gga ggt 2183 Ser Arg Ser Leu Leu Ser Ala Asn Thr Thr Thr Pro Arg Thr Gly Gly 275 280 285 290 gag ctc cgt ggt ctt cct aat aac aac ccc tgg gtg gta act ggg ttt 2231 Glu Leu Arg Gly Leu Pro Asn Asn Asn Pro Trp Val Val Thr Gly Phe 295 300 305 gta acg cta ggt gta gtt gcg ggt ggg ctt gtg ctt gtc ctt tgc tac 2279 Val Thr Leu Gly Val Val Ala Gly Gly Leu Val Leu Val Leu Cys Tyr 310 315 320 cta tac aca cct tgc tgt gct tat tta gta atc ttg tgt tgc tgg ttt 2327 Leu Tyr Thr Pro Cys Cys Ala Tyr Leu Val Ile Leu Cys Cys Trp Phe 325 330 335 aag aaa tgg ggg ccc tac tagtcgcgct tgctttactt tcacttttgg 2375 Lys Lys Trp Gly Pro Tyr 340 atctgggctc tactatgcta gttcagcctg tactatttga tccatgcctc aattttgatc 2435 cagacaactg cacactcact tttgctccag aggctggccg ctgtggagtt cttattaggt 2495 gcggacggga atgcagtccc attgaaatac accacaataa caaaatttgg aacaatacct 2555 tattcaccac atggcagcca ggagaccctg agtggtatac tgtctctgtc cgtggtcctg 2615 acggttccat ccgcactgct aataacactt ttatttttgc tgagatgtgc gatctgacca 2675 tgttcatgag caaacagtat aacctatggc ctccaagcaa ggagaacatt gtggcattct 2735 ccattgctta tttcttgtgt acgtgtctca ttactgctat tctatgtatc tgcatacact 2795 tgcttatttg ccaccgccac agaaacagca atgaggaaaa agagaaaatg ccttgagctt 2855 tttctcattt ttgttttttt ttgtttacag ccatggcttc agttatagct ctaattattg 2915 tcagcattct cactgccgca cagggacaaa caattgtcta tattacctta ggtcataacc 2975 acactcttat aggaccccaa attagttcac aggttatatg gaccaaactt ggaagtgttg 3035 attattttga cataatctgc aacagaacta aaccaatatt tgtaacctgt aacaaacaaa 3095 atctcacctt aattaatgtt agcgaaattt acagcggtta ctattatggt tatgacagac 3155 acagcagtga atataaaaat tacctagttc gcataactca acccaaaacc acaaaaatgc 3215 caaataaggc aaaaattcaa atggttagcg cattagaaca tcttacatat cccaccacac 3275 ccgatgagag aaacattcca aattcaatga ttgccattat tgcggcggtg gcagtgggaa 3335 tggcactaat aataatttgt atgttcctat atgcttgtta ctgtagaaag tttcatcaca 3395 aacaggattc cctactaaat ttttgacatt taatttttta tacagctatg gtttccacta 3455 cagccttttt tgttattagt agccttgcag ctgtcactta tggtcgctca cacctcactg 3515 taactgttgg ctcaacttgt acactacaag gaccccaaga agggcatgtc agttggtgga 3575 gaatatatga tagtggatgg ttcattaggc catgtgacca gcctggtaac aaatttttct 3635 gcaacgggag agacttgacc attattaaca tcacagtaaa tgaccagggc ttctattatg 3695 gaactaacta taaaaataac ttagattaca acattatcgt agtgccagcc accactccag 3755 ctccccgcaa aaccactttc tttagcagca gtgccagtat ttctaaaaca gcttctgcaa 3815 gcttcaaaaa attcgcttta cgtaattcca caacctcttc cacttccaat aatacaatgt 3875 ctaaatcagt aatcggcatc gctgctgccg cgatagtggg attaatgatt ataattctat 3935 gcataatcta ctacgcctgc tgctatagaa aacaacatga acaaaaaacc gatcccttgc 3995 tgaattttga tatttaattt ttttatagaa tcatgaaaaa actaagtatc ctagctttca 4055 ttttgtttca aacatttacc aatgtgcaga ctactttaag tcatggtata gagaaccaca 4115 ctacctctta tgagctcaca aacattacta cccatcatcc taaatatgct atgcaactag 4175 aaatcaccat gctaattgta gttggaatac ttatcctagc tattattttc tattttacac 4235 tatgccgcca aatacctaat attcataaaa attctaaaag acgtcccatc tattgccctg 4295 tgattagtcg accccatatg actctaaatg aaatctaaga tcatctattt ctctttttta 4355 cagtatggtg aacaccaatc atg att cct aga aat ttc ttc ttc acc ata ctc 4408 Met Ile Pro Arg Asn Phe Phe Phe Thr Ile Leu 345 350 355 atc tgt gct ttt aat gtc tgt gcc acc ttc aca gca gta gcc act gca 4456 Ile Cys Ala Phe Asn Val Cys Ala Thr Phe Thr Ala Val Ala Thr Ala 360 365 370 acc cca gac tgt ata gga gca ttt gcc tca tat aca ctt ttc gct ttt 4504 Thr Pro Asp Cys Ile Gly Ala Phe Ala Ser Tyr Thr Leu Phe Ala Phe 375 380 385 gtc gct tgc acc tgc gtg tgt agc gta gtc tgc ctg gtt att aat ttt 4552 Val Ala Cys Thr Cys Val Cys Ser Val Val Cys Leu Val Ile Asn Phe 390 395 400 ttc caa ctt gta gac tgg atc ttt gta cga ctt gcc tac ctg cgt cac 4600 Phe Gln Leu Val Asp Trp Ile Phe Val Arg Leu Ala Tyr Leu Arg His 405 410 415 cat ccc gaa tac cgc aat caa cat gtt gcg gca ctt ctc aga ctt att 4648 His Pro Glu Tyr Arg Asn Gln His Val Ala Ala Leu Leu Arg Leu Ile 420 425 430 435 taaaaccatg caggctatac taccagtcat tctgcttctg ttgctcccct gcgatgcctt 4708 aacccccgtc gctaatcgta ccccacctga acaacttaga aaatgcaaat tccaacaacc 4768 atggacattc cttgattgct accgagaaaa atctgatttc cctacatact ggattatgat 4828 cattggaatt gtcaatctag tttcttgcac actattctct ttccttgttt atcatttttt 4888 tgattttgga tggaatgccc ccaatgcact cacttaccca caagaaccag aggaacatat 4948 cccactacag aacatgcaac agccaatagc tataatagat tatgacaatg agccacagcc 5008 ctcgctgctt cctgctatta gttacttcaa cctaaccggt ggag atg act gac cca 5064 Met Thr Asp Pro ctc gcc gcc tcc act gct gcc gag gaa cta ctt gat atg gac ggc cgc 5112 Leu Ala Ala Ser Thr Ala Ala Glu Glu Leu Leu Asp Met Asp Gly Arg 440 445 450 455 gcc tca gaa cag cga ctc gcc caa cta cgc att cgc cag cag cag gaa 5160 Ala Ser Glu Gln Arg Leu Ala Gln Leu Arg Ile Arg Gln Gln Gln Glu 460 465 470 cgt gcc gcc aag gag ctc agg gat gct att gag att cac cag tgc aaa 5208 Arg Ala Ala Lys Glu Leu Arg Asp Ala Ile Glu Ile His Gln Cys Lys 475 480 485 aaa ggc ata ttc tgc ttg gta aaa caa gcc aag atc tcc tac gag atc 5256 Lys Gly Ile Phe Cys Leu Val Lys Gln Ala Lys Ile Ser Tyr Glu Ile 490 495 500 acc gct aac gac cac cgc ctc tca tat gag ctt ggc ccg cag cgt cag 5304 Thr Ala Asn Asp His Arg Leu Ser Tyr Glu Leu Gly Pro Gln Arg Gln 505 510 515 aaa ttc act tgc atg gtg gga atc aac ccc ata gtc atc acc cag caa 5352 Lys Phe Thr Cys Met Val Gly Ile Asn Pro Ile Val Ile Thr Gln Gln 520 525 530 535 gcc gga gat acc aag ggt tgc atc cat tgt tcc tgt gaa tcc acc gag 5400 Ala Gly Asp Thr Lys Gly Cys Ile His Cys Ser Cys Glu Ser Thr Glu 540 545 550 tgc atc tac acc cta ctg aag acc ctc tgc ggc ctt cga gac ctc cta 5448 Cys Ile Tyr Thr Leu Leu Lys Thr Leu Cys Gly Leu Arg Asp Leu Leu 555 560 565 ccc atg aac taatcacccc cgc 5470 Pro Met Asn 570 <210> 63 <211> 198 <212> PRT <213> Simian adenovirus 27 <400> 63 Met Ser Gln Arg Arg Gly Asn Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Thr Glu Glu Met Glu 20 25 30 Asp Trp Asp Ser Gln Ala Glu Glu Glu Glu Asp Ser Leu Glu Glu Asp 35 40 45 Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu Ala Thr 50 55 60 Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Ala Thr Asp 65 70 75 80 Asn Thr Thr Ala Thr Ile Ser Val Pro Gly Arg Gly Val Gln His Arg 85 90 95 Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Leu Pro Asn Ala Thr Thr 100 105 110 Ala Ser Lys Thr Gly Lys Lys Glu Arg Gln Gly Tyr Lys Ser Trp Arg 115 120 125 Gly His Lys Asn Ala Ile Ile Ser Cys Leu His Glu Cys Gly Gly Asn 130 135 140 Ile Ser Phe Thr Arg Arg Tyr Leu Leu Phe His His Gly Val Asn Phe 145 150 155 160 Pro Arg Asn Val Leu His Tyr Tyr Arg His Leu His Ser Pro Tyr Tyr 165 170 175 Ser Gln Gln Ala Ser Ala Glu Lys Asp Asn Ser Ser Lys Asn Leu Gln 180 185 190 Gln Lys Thr Ser Ser Ser 195 <210> 64 <211> 146 <212> PRT <213> Simian adenovirus 27 <400> 64 Met Lys Ala Phe Ala Val Leu Phe Val Leu Ser Leu Ile Lys Thr Glu 1 5 10 15 Leu Arg Leu Ser Tyr Gly Leu Pro Ile Leu Gln Leu Gly Leu Tyr Asn 20 25 30 Asn Gln Thr Leu Arg Ser Ser Gln Lys Thr Pro Thr Leu Pro Leu Ile 35 40 45 Gln Glu Ser Asn Ser Thr Ser Pro Ala Pro His Phe Thr Ser Leu Pro 50 55 60 Glu Thr Asn Asn Leu Gly Ala Gln Leu His His Phe Ser Arg Ser Leu 65 70 75 80 Leu Ser Ala Asn Thr Thr Thr Pro Arg Thr Gly Gly Glu Leu Arg Gly 85 90 95 Leu Pro Asn Asn Asn Pro Trp Val Val Thr Gly Phe Val Thr Leu Gly 100 105 110 Val Val Ala Gly Gly Leu Val Leu Val Leu Cys Tyr Leu Tyr Thr Pro 115 120 125 Cys Cys Ala Tyr Leu Val Ile Leu Cys Cys Trp Phe Lys Lys Trp Gly 130 135 140 Pro Tyr 145 <210> 65 <211> 91 <212> PRT <213> Simian adenovirus 27 <400> 65 Met Ile Pro Arg Asn Phe Phe Phe Thr Ile Leu Ile Cys Ala Phe Asn 1 5 10 15 Val Cys Ala Thr Phe Thr Ala Val Ala Thr Ala Thr Pro Asp Cys Ile 20 25 30 Gly Ala Phe Ala Ser Tyr Thr Leu Phe Ala Phe Val Ala Cys Thr Cys 35 40 45 Val Cys Ser Val Val Cys Leu Val Ile Asn Phe Phe Gln Leu Val Asp 50 55 60 Trp Ile Phe Val Arg Leu Ala Tyr Leu Arg His His Pro Glu Tyr Arg 65 70 75 80 Asn Gln His Val Ala Ala Leu Leu Arg Leu Ile 85 90 <210> 66 <211> 135 <212> PRT <213> Simian adenovirus 27 <400> 66 Met Thr Asp Pro Leu Ala Ala Ser Thr Ala Ala Glu Glu Leu Leu Asp 1 5 10 15 Met Asp Gly Arg Ala Ser Glu Gln Arg Leu Ala Gln Leu Arg Ile Arg 20 25 30 Gln Gln Gln Glu Arg Ala Ala Lys Glu Leu Arg Asp Ala Ile Glu Ile 35 40 45 His Gln Cys Lys Lys Gly Ile Phe Cys Leu Val Lys Gln Ala Lys Ile 50 55 60 Ser Tyr Glu Ile Thr Ala Asn Asp His Arg Leu Ser Tyr Glu Leu Gly 65 70 75 80 Pro Gln Arg Gln Lys Phe Thr Cys Met Val Gly Ile Asn Pro Ile Val 85 90 95 Ile Thr Gln Gln Ala Gly Asp Thr Lys Gly Cys Ile His Cys Ser Cys 100 105 110 Glu Ser Thr Glu Cys Ile Tyr Thr Leu Leu Lys Thr Leu Cys Gly Leu 115 120 125 Arg Asp Leu Leu Pro Met Asn 130 135 <210> 67 <211> 920 <212> DNA <213> Simian adenovirus 27 <220> <221> CDS <222> (35)..(611) <223> label=Ela <220> <221> CDS <222> (705)..(910) <223> label=Ela <400> 67 ccgcgccgcg agtcagatct ccactttgaa aaaa atg aga cac ctg cga ttc ctg 55 Met Arg His Leu Arg Phe Leu 1 5 cct cag gaa atc tcc att gag acc ggg gat gaa ata ctg cag ttt gtg 103 Pro Gln Glu Ile Ser Ile Glu Thr Gly Asp Glu Ile Leu Gln Phe Val 10 15 20 gta aat gcc ctg atg gga gac gat ccg gag ccg cct gcg cag cct ttc 151 Val Asn Ala Leu Met Gly Asp Asp Pro Glu Pro Pro Ala Gln Pro Phe 25 30 35 gat cct cct acg ctt cat gaa ctg tat gat tta gag gta gac ggg ccg 199 Asp Pro Pro Thr Leu His Glu Leu Tyr Asp Leu Glu Val Asp Gly Pro 40 45 50 55 gag gac cct aac gag gaa gct gtg aat ggg ttt ttc agc gat tct atg 247 Glu Asp Pro Asn Glu Glu Ala Val Asn Gly Phe Phe Ser Asp Ser Met 60 65 70 cta tta gct gct agt gaa gga gtg gac tta gac cca cct tct gag acc 295 Leu Leu Ala Ala Ser Glu Gly Val Asp Leu Asp Pro Pro Ser Glu Thr 75 80 85 ctt gat acc cca ggg gtg gtg gtg gaa agc ggc gga ggt ggg aaa aaa 343 Leu Asp Thr Pro Gly Val Val Val Glu Ser Gly Gly Gly Gly Lys Lys 90 95 100 ttg cct gaa ctt ggt gct gct gaa atg gat ttg cac tgt tat gaa gag 391 Leu Pro Glu Leu Gly Ala Ala Glu Met Asp Leu His Cys Tyr Glu Glu 105 110 115 ggc ttt cct ccg agt gat gat gaa gat gag gaa aat gtg cag tcg atc 439 Gly Phe Pro Pro Ser Asp Asp Glu Asp Glu Glu Asn Val Gln Ser Ile 120 125 130 135 cag acc gca gcg ggt gag gga ata aga gct gcc aat gat ggt ttt aag 487 Gln Thr Ala Ala Gly Glu Gly Ile Arg Ala Ala Asn Asp Gly Phe Lys 140 145 150 ttg gac tac ccg gag ctg cct gga cat ggc tgt aag tct tgt gaa ttt 535 Leu Asp Tyr Pro Glu Leu Pro Gly His Gly Cys Lys Ser Cys Glu Phe 155 160 165 cac agg aat agt act gga cta aaa gaa ctg ttg tgc tcg ctt tgc tat 583 His Arg Asn Ser Thr Gly Leu Lys Glu Leu Leu Cys Ser Leu Cys Tyr 170 175 180 atg aga acg cac tgc cat ttt att tac a gtaagtgtgt ttaacttaaa 631 Met Arg Thr His Cys His Phe Ile Tyr 185 190 tttaaaggga cagtgtagca gtgttaataa ctgtgaatgt gggatttatg tttttgcttg 691 tgatttttta tag gt cct gtg tct gat gct gat gaa tcg cct tct cct 739 Ser Pro Val Ser Asp Ala Asp Glu Ser Pro Ser Pro 195 200 gat tca act acc tca cct cct gaa att cag gcg cca gtc cct gca aac 787 Asp Ser Thr Thr Ser Pro Pro Glu Ile Gln Ala Pro Val Pro Ala Asn 205 210 215 220 ata tgc aag ccc att cct gtg aag gct aag cct ggg aaa cgc cct gct 835 Ile Cys Lys Pro Ile Pro Val Lys Ala Lys Pro Gly Lys Arg Pro Ala 225 230 235 gtg gat aag ctg gag gac ttg ctt gag ggt ggg gat gga cct ttg gac 883 Val Asp Lys Leu Glu Asp Leu Leu Glu Gly Gly Asp Gly Pro Leu Asp 240 245 250 ttg agt acc cgg aaa ctg cca agg caa tgagtgccct 920 Leu Ser Thr Arg Lys Leu Pro Arg Gln 255 260 <210> 68 <211> 261 <212> PRT <213> Simian adenovirus 27 <400> 68 Met Arg His Leu Arg Phe Leu Pro Gln Glu Ile Ser Ile Glu Thr Gly 1 5 10 15 Asp Glu Ile Leu Gln Phe Val Val Asn Ala Leu Met Gly Asp Asp Pro 20 25 30 Glu Pro Pro Ala Gln Pro Phe Asp Pro Pro Thr Leu His Glu Leu Tyr 35 40 45 Asp Leu Glu Val Asp Gly Pro Glu Asp Pro Asn Glu Glu Ala Val Asn 50 55 60 Gly Phe Phe Ser Asp Ser Met Leu Leu Ala Ala Ser Glu Gly Val Asp 65 70 75 80 Leu Asp Pro Pro Ser Glu Thr Leu Asp Thr Pro Gly Val Val Val Glu 85 90 95 Ser Gly Gly Gly Gly Lys Lys Leu Pro Glu Leu Gly Ala Ala Glu Met 100 105 110 Asp Leu His Cys Tyr Glu Glu Gly Phe Pro Pro Ser Asp Asp Glu Asp 115 120 125 Glu Glu Asn Val Gln Ser Ile Gln Thr Ala Ala Gly Glu Gly Ile Arg 130 135 140 Ala Ala Asn Asp Gly Phe Lys Leu Asp Tyr Pro Glu Leu Pro Gly His 145 150 155 160 Gly Cys Lys Ser Cys Glu Phe His Arg Asn Ser Thr Gly Leu Lys Glu 165 170 175 Leu Leu Cys Ser Leu Cys Tyr Met Arg Thr His Cys His Phe Ile Tyr 180 185 190 Ser Pro Val Ser Asp Ala Asp Glu Ser Pro Ser Pro Asp Ser Thr Thr 195 200 205 Ser Pro Pro Glu Ile Gln Ala Pro Val Pro Ala Asn Ile Cys Lys Pro 210 215 220 Ile Pro Val Lys Ala Lys Pro Gly Lys Arg Pro Ala Val Asp Lys Leu 225 230 235 240 Glu Asp Leu Leu Glu Gly Gly Asp Gly Pro Leu Asp Leu Ser Thr Arg 245 250 255 Lys Leu Pro Arg Gln 260 <210> 69 <211> 880 <212> DNA <213> Simian adenovirus 27 <220> <221> CDS <222> (9)..(357) <223> label=33K <220> <221> CDS <222> (527)..(876) <223> label=33K <400> 69 ctctcagg atg tct cag cgc cga gga aac aag aag ttg aaa gtg cag ctg 50 Met Ser Gln Arg Arg Gly Asn Lys Lys Leu Lys Val Gln Leu 1 5 10 ccg ccc cca gag gat atg gag gaa gac tgg gac agt cag aca gag gag 98 Pro Pro Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Thr Glu Glu 15 20 25 30 atg gaa gat tgg gac agc cag gca gag gag gag gag gac agc ctg gag 146 Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Glu Glu Asp Ser Leu Glu 35 40 45 gaa gac agt ttg gag gag gaa gac gag gag gca gag gag gtg gaa gaa 194 Glu Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu 50 55 60 gca acc gcc gcc aaa cag ttg tcc tcg gcg gcg gag aca agc aag gcc 242 Ala Thr Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Ala 65 70 75 aca gac aac acc aca gct acc atc tcc gtt ccg ggt cgg ggg gtc cag 290 Thr Asp Asn Thr Thr Ala Thr Ile Ser Val Pro Gly Arg Gly Val Gln 80 85 90 cac cgt ccc aac agt aga tgg gat gag acc ggg cga ctc ccg aat gcg 338 His Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Leu Pro Asn Ala 95 100 105 110 acc acc gct tct aag act g gtaagaagga gcggcaggga tacaagtcct 387 Thr Thr Ala Ser Lys Thr 115 ggcgggggca taagaacgct atcatatcct gcttgcatga atgcgggggc aacatatcct 447 tcacccgccg ctacctgctc ttccaccacg gggtgaactt cccccgcaat gtcttgcatt 507 actaccgtca cctccacag cc cct act aca gcc agc aag cct cgg cag aaa 558 Ala Pro Thr Thr Ala Ser Lys Pro Arg Gln Lys 120 125 aag aca aca gca gca aga acc tcc agc aga aaa cca gca gca gtt aga 606 Lys Thr Thr Ala Ala Arg Thr Ser Ser Arg Lys Pro Ala Ala Val Arg 130 135 140 aca ccc aca gca ggt gca aca gga gga gga ctg aga atc aca gcg aac 654 Thr Pro Thr Ala Gly Ala Thr Gly Gly Gly Leu Arg Ile Thr Ala Asn 145 150 155 gag cca gcg cag acc cga gag ctg aga aac cgg att ttt cca acc ctc 702 Glu Pro Ala Gln Thr Arg Glu Leu Arg Asn Arg Ile Phe Pro Thr Leu 160 165 170 175 tat gcc atc ttc caa cag agt cgg ggg caa gag cag gaa ctg aaa gta 750 Tyr Ala Ile Phe Gln Gln Ser Arg Gly Gln Glu Gln Glu Leu Lys Val 180 185 190 aaa aac cga tct ttg cgc tcg ctc acc cga agt tgt ttg tat cac aag 798 Lys Asn Arg Ser Leu Arg Ser Leu Thr Arg Ser Cys Leu Tyr His Lys 195 200 205 agc gaa gac caa ctt cag cgc act ctc gag gac gcc gag gct ctc ttc 846 Ser Glu Asp Gln Leu Gln Arg Thr Leu Glu Asp Ala Glu Ala Leu Phe 210 215 220 aac aag tac tgc gcg ctc act ctt aaa gag tagc 880 Asn Lys Tyr Cys Ala Leu Thr Leu Lys Glu 225 230 <210> 70 <211> 233 <212> PRT <213> Simian adenovirus 27 <400> 70 Met Ser Gln Arg Arg Gly Asn Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Thr Glu Glu Met Glu 20 25 30 Asp Trp Asp Ser Gln Ala Glu Glu Glu Glu Asp Ser Leu Glu Glu Asp 35 40 45 Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu Ala Thr 50 55 60 Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Ala Thr Asp 65 70 75 80 Asn Thr Thr Ala Thr Ile Ser Val Pro Gly Arg Gly Val Gln His Arg 85 90 95 Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Leu Pro Asn Ala Thr Thr 100 105 110 Ala Ser Lys Thr Ala Pro Thr Thr Ala Ser Lys Pro Arg Gln Lys Lys 115 120 125 Thr Thr Ala Ala Arg Thr Ser Ser Arg Lys Pro Ala Ala Val Arg Thr 130 135 140 Pro Thr Ala Gly Ala Thr Gly Gly Gly Leu Arg Ile Thr Ala Asn Glu 145 150 155 160 Pro Ala Gln Thr Arg Glu Leu Arg Asn Arg Ile Phe Pro Thr Leu Tyr 165 170 175 Ala Ile Phe Gln Gln Ser Arg Gly Gln Glu Gln Glu Leu Lys Val Lys 180 185 190 Asn Arg Ser Leu Arg Ser Leu Thr Arg Ser Cys Leu Tyr His Lys Ser 195 200 205 Glu Asp Gln Leu Gln Arg Thr Leu Glu Asp Ala Glu Ala Leu Phe Asn 210 215 220 Lys Tyr Cys Ala Leu Thr Leu Lys Glu 225 230 <210> 71 <211> 35646 <212> DNA <213> Simian adenovirus 29 <220> <221> repeat_region <222> (1)..(132) <223> label=ITR <220> <221> CDS <222> (1920)..(3404) <223> label=Elb\55K <220> <221> CDS <222> (3499)..(3912) <223> label=pIX <220> <221> misc_feature <222> (3982)..(5603) <223> complement(3982..5312,5591..5603) label=IVa2 <220> <221> misc_feature <222> (5085)..(13918) <223> complement(5085..8657,13910..13918) label=pol <220> <221> misc_feature <222> (8459)..(13918) <223> complement(8459..10438,13910..13918) label=pTP <220> <221> CDS <222> (10922)..(12088) <223> label=52K <220> <221> CDS <222> (12116)..(13876) <223> label=pIIIa <220> <221> CDS <222> (13963)..(15690) <223> label=penton <220> <221> CDS <222> (15697)..(16272) <223> label=pVII <220> <221> CDS <222> (16318)..(17367) <223> label=V <220> <221> CDS <222> (17399)..(17623) <223> label=pX <220> <221> CDS <222> (17699)..(18448) <223> label=pVI <220> <221> CDS <222> (18570)..(21431) <223> label=hexon <220> <221> CDS <222> (21462)..(22088) <223> label=protease <220> <221> misc_feature <222> (22182)..(23729) <223> complement label=DBP <220> <221> CDS <222> (23760)..(26255) <223> label=100K <220> <221> CDS <222> (26909)..(27589) <223> label=pVIII <220> <221> CDS <222> (27863)..(28306) <223> label=E3\CR1-alpha <220> <221> CDS <222> (28683)..(29420) <223> label=E3\CR1-beta <220> <221> CDS <222> (29442)..(30005) <223> label=E3\CR1-gamma <220> <221> CDS <222> (30024)..(30344) <223> label=E3\CR1-delta <220> <221> CDS <222> (30377)..(30649) <223> label=E3\RID-alpha <220> <221> CDS <222> (31039)..(31434) <223> label=E3\14.7K <220> <221> CDS <222> (31663)..(32634) <223> label=fiber <220> <221> misc_feature <222> (32678)..(33852) <223> complement(32678..32926,33649..33852) label=E4\orf6/7 <220> <221> misc_feature <222> (32926)..(33852) <223> complement label=E4\orf6 <220> <221> misc_feature <222> (33728)..(34108) <223> complement label=E4\orf4 <220> <221> misc_feature <222> (34121)..(34471) <223> complement label=E4\orf3 <220> <221> misc_feature <222> (34471)..(34857) <223> complement label=E4\orf2 <220> <221> misc_feature <222> (34899)..(35270) <223> complement label=E4\orf1 <220> <221> repeat_region <222> (35515)..(35646) <223> complement label=ITR <400> 71 catcatcaat aatatacctt ataaatggaa cggtgccaac atgcaaatga gcttttgaaa 60 atggagggcg gaaggggatt ggccagcggg ttcaacggtc aaaaggggcg ggccggcgcg 120 gggaggtgac gtatttcgtg tgggaggagt tatgttgcaa gttatcgcgg caaaagtgac 180 gtaaaacgag gtgtggtttg aacacggaag tagacagttt tcccgcgctg actgacagga 240 tatgaggtag ttttgggcgg atgcaagtga aaattctcca ttttcgcgcg aaaactgaat 300 gaggaagtga atttctgagt aatttcgagt ttatgacagg gtggagtatt taccgagggc 360 cgagtagact ttgaccgatt acgtggaggt ttcgattacc gtgtttttca cctaaatttc 420 cgcgtacggt gtcaaagtcc tgtgttttta cgtaggcgtc agctgatcgc tagggtattt 480 aaacctgacg agttccgtca agaggccact cttgagtgcc agcgagaaga gatttctcct 540 ccgcgccgcg agtcagatct ccactttgaa aaaatgagac acctgcgatt cctgcctcag 600 gaaatctcca tcgagaccgg gaatgaaata ctacagcttg tggtaaatgc cctgatggga 660 gacgatccgg agccgcctgc gcatccgttc gatcctccta cgcttcatga actgtatgat 720 ttagaggtag atgggccgga tgatcctaac gaggaagctg tgaatggttt ttttagcgaa 780 tctatgctat tggctgctaa tgaaggagtg gacatagacc caccttctga gaccctcgat 840 accccagggg tgattgtgga gagcggcaga ggtgggaaaa aattgcctga acttggtgct 900 gctgaaatgg acttgcactg ttatgaagag ggttttcctc cgagtgatga tgaagaggag 960 gaaaatgtgc agtcgatcca gaccgcagcg ggtgagggaa tgaaagctgc caatgatggt 1020 tttaagttgg actgcccgga gctgcctgga catggctgta agtcttgtga atttcacagg 1080 aatagtactg gactaaaaga actgttgtgc tcgctttgct atatgagaac gcactgccat 1140 tttatttaca gtaagtgtgt ctaacttaaa tttaaaggga cagtgtagca gtttaatgtc 1200 tgttgaatgt gggatttatg tttttgtgat ttttataggt cctgtgtctg atgctgatga 1260 atcgccttct cctgattcaa ctacctcacc tcctgaaatt caggcgccag tccctgcaaa 1320 cgtatgcaag cccattcctg tgaaggctaa gcctgggaaa cgccctgctg tggataaact 1380 ggaggacttg cttgagggtg gggatggacc tttggacttg agtacccgga aactgccaag 1440 gcaatgagtg ccctgcacct gtgtttattt aatgtgacgt cagtatttat gtgagagtgc 1500 catgtaataa aattatgtca gctgctgagt attttattgc ttcttgggtg gggacttgga 1560 tatataagta ggagcagacc tgtgtggtta gctcacagca gcttgctgcc atccatggag 1620 gtttgggcta tcttggaaga tctcaggcag actagacaac tgctagaaaa cgcctcggac 1680 ggagtctcta gtctttggag attctggttc ggtggtgatc tagctaggct agtctttagg 1740 gtaaaacggg agtatagtga agaatttgaa aagttattgg aagacagtcc aggacttttt 1800 gaagccctta acttgggcca ccaggctcat tttaaggaga aggttttatc agttttagat 1860 ttttctaccc ctggtagaac tgctgctgct gtagctttcc ttacttttat attggataa 1919 atg gat ccc aca aac cca ctt cag caa ggg ata cgt ctt gga ttt cat 1967 Met Asp Pro Thr Asn Pro Leu Gln Gln Gly Ile Arg Leu Gly Phe His 1 5 10 15 agc agc agc ttt gtg gag aac atg gaa ggc ccg cag gct gag gat aat 2015 Ser Ser Ser Phe Val Glu Asn Met Glu Gly Pro Gln Ala Glu Asp Asn 20 25 30 ctt aga tta ctg gcc agt gca gcc tct ggg cgt agc agc aat cct gag 2063 Leu Arg Leu Leu Ala Ser Ala Ala Ser Gly Arg Ser Ser Asn Pro Glu 35 40 45 aca ccc acc ggc cat gcc agc ggt ttt gga gga gga gca gca gga gga 2111 Thr Pro Thr Gly His Ala Ser Gly Phe Gly Gly Gly Ala Ala Gly Gly 50 55 60 caa ccc gag agc cgg ctt gga ccc tcc ggt gga gga ggc gga gga gta 2159 Gln Pro Glu Ser Arg Leu Gly Pro Ser Gly Gly Gly Gly Gly Gly Val 65 70 75 80 gct gac ctg ttt cct gaa ctg cga cgg gtg ctt act agg tct acg tcc 2207 Ala Asp Leu Phe Pro Glu Leu Arg Arg Val Leu Thr Arg Ser Thr Ser 85 90 95 agt gga cag gac agg ggc att aag agg gag agg aat gct agt ggg cat 2255 Ser Gly Gln Asp Arg Gly Ile Lys Arg Glu Arg Asn Ala Ser Gly His 100 105 110 aat tca aga act gag ttg gct tta agt tta atg agt cgc agc cgc cct 2303 Asn Ser Arg Thr Glu Leu Ala Leu Ser Leu Met Ser Arg Ser Arg Pro 115 120 125 gaa act atc tgg tgg cat gag gtt cag agc gag ggc agg gat gaa gtt 2351 Glu Thr Ile Trp Trp His Glu Val Gln Ser Glu Gly Arg Asp Glu Val 130 135 140 tca ata ttg cag gaa aaa tat tct cta gaa caa att aaa acc tgt tgg 2399 Ser Ile Leu Gln Glu Lys Tyr Ser Leu Glu Gln Ile Lys Thr Cys Trp 145 150 155 160 ttg gaa cct gag gat gat tgg gag gtg gcc att agg aat tat gct aag 2447 Leu Glu Pro Glu Asp Asp Trp Glu Val Ala Ile Arg Asn Tyr Ala Lys 165 170 175 ata tct ctg agg cct gat aaa cag tat aaa att acc aaa aag att aat 2495 Ile Ser Leu Arg Pro Asp Lys Gln Tyr Lys Ile Thr Lys Lys Ile Asn 180 185 190 atc aga aat gca tgc tac ata gca ggg aat ggg gcc gag gtt ata ata 2543 Ile Arg Asn Ala Cys Tyr Ile Ala Gly Asn Gly Ala Glu Val Ile Ile 195 200 205 gat aca cca gat aaa aca gct ttt agg tgt tgc atg atg ggt atg tgg 2591 Asp Thr Pro Asp Lys Thr Ala Phe Arg Cys Cys Met Met Gly Met Trp 210 215 220 cca ggg gtg gct ggc atg gag gca gtg acc ctt atg aat ata agg ttt 2639 Pro Gly Val Ala Gly Met Glu Ala Val Thr Leu Met Asn Ile Arg Phe 225 230 235 240 agg gga gat ggg tat aat ggg att gtc ttt atg gct aac act aag ctg 2687 Arg Gly Asp Gly Tyr Asn Gly Ile Val Phe Met Ala Asn Thr Lys Leu 245 250 255 att ctg cat ggt tgt agc ttt ttt ggg ttt aat aat act tgt gtg gaa 2735 Ile Leu His Gly Cys Ser Phe Phe Gly Phe Asn Asn Thr Cys Val Glu 260 265 270 tct tgg gga caa gtc agt atc agg ggt tgt agt ttc tat gca tgc tgg 2783 Ser Trp Gly Gln Val Ser Ile Arg Gly Cys Ser Phe Tyr Ala Cys Trp 275 280 285 att gca cta tca ggc aga acc aag agt cag ttg tct gtg aag aaa tgc 2831 Ile Ala Leu Ser Gly Arg Thr Lys Ser Gln Leu Ser Val Lys Lys Cys 290 295 300 atg ttc gag aga tgt aac ttg ggc ata ctg aat gaa ggt gaa gca agg 2879 Met Phe Glu Arg Cys Asn Leu Gly Ile Leu Asn Glu Gly Glu Ala Arg 305 310 315 320 gtc cgc cac tgt gct gct aca gaa act ggc tgc ttc att cta ata aag 2927 Val Arg His Cys Ala Ala Thr Glu Thr Gly Cys Phe Ile Leu Ile Lys 325 330 335 gga aat gcc agt gtg aag cat aac atg atc tgt gga ccc tcg gat gag 2975 Gly Asn Ala Ser Val Lys His Asn Met Ile Cys Gly Pro Ser Asp Glu 340 345 350 agg cct tat cag atg ctg acc tgt gct gga gga cat tgc aat atg ctg 3023 Arg Pro Tyr Gln Met Leu Thr Cys Ala Gly Gly His Cys Asn Met Leu 355 360 365 gct act gtg cat att gtt tct cat gca cgc aag aaa tgg cct gtt ttt 3071 Ala Thr Val His Ile Val Ser His Ala Arg Lys Lys Trp Pro Val Phe 370 375 380 gaa cat aat gtg atg acc aag tgc acc atg cac gca ggt ggt cgc agg 3119 Glu His Asn Val Met Thr Lys Cys Thr Met His Ala Gly Gly Arg Arg 385 390 395 400 gga atg ttt atg cct tac cag tgt aac atg aat cat gtg aag gtg atg 3167 Gly Met Phe Met Pro Tyr Gln Cys Asn Met Asn His Val Lys Val Met 405 410 415 ttg gaa cca gat gcc ttt tcc aga atg agc tta aca gga atc ttt gat 3215 Leu Glu Pro Asp Ala Phe Ser Arg Met Ser Leu Thr Gly Ile Phe Asp 420 425 430 atg aat gtg caa cta tgg aag atc ctg aga tat gat gag acc aaa tcg 3263 Met Asn Val Gln Leu Trp Lys Ile Leu Arg Tyr Asp Glu Thr Lys Ser 435 440 445 agg gtg cgc gca tgc gag tgc ggg ggc aag cat gcc agg ttc cag ccg 3311 Arg Val Arg Ala Cys Glu Cys Gly Gly Lys His Ala Arg Phe Gln Pro 450 455 460 gtg tgt gtg gat gtg acg gaa gac ctg aga ccc gat cat ttg gtg ctt 3359 Val Cys Val Asp Val Thr Glu Asp Leu Arg Pro Asp His Leu Val Leu 465 470 475 480 gcc tgc act gga gcg gag ttc ggt tct agt ggg gaa gaa act gac 3404 Ala Cys Thr Gly Ala Glu Phe Gly Ser Ser Gly Glu Glu Thr Asp 485 490 495 taaagtgagt agtggggaat gctgtggagg ggcttccagg cgggtaaggt gggcagattg 3464 ggtaaattct gtttctttct gtcttgcagc tgcc atg agt gga agc gct tct ttt 3519 Met Ser Gly Ser Ala Ser Phe 500 gag ggg gga gtc ttt agc cct tat ctg acg ggg cga ctc cca ccc tgg 3567 Glu Gly Gly Val Phe Ser Pro Tyr Leu Thr Gly Arg Leu Pro Pro Trp 505 510 515 gca gga gtt cgt cag aat gtc atg gga tcc act gtg gat ggg aga ccc 3615 Ala Gly Val Arg Gln Asn Val Met Gly Ser Thr Val Asp Gly Arg Pro 520 525 530 gtc cag ccc gcc aat tcc tca acg ctg acc tat gcc act ttg agc tct 3663 Val Gln Pro Ala Asn Ser Ser Thr Leu Thr Tyr Ala Thr Leu Ser Ser 535 540 545 550 tca tcc ttg gat gca gct gca gcc gct gcc gcc tct gct gcc gcc aac 3711 Ser Ser Leu Asp Ala Ala Ala Ala Ala Ala Ala Ser Ala Ala Ala Asn 555 560 565 act gtc ctt gga atg ggc tat tat gga agc atc gtt gcc aat tcc agt 3759 Thr Val Leu Gly Met Gly Tyr Tyr Gly Ser Ile Val Ala Asn Ser Ser 570 575 580 tcc tca aat aac cct tcg acc ctg gct gag gac aag cta ctt gtc ctc 3807 Ser Ser Asn Asn Pro Ser Thr Leu Ala Glu Asp Lys Leu Leu Val Leu 585 590 595 ttg gct cag ctc gag gcc ttg acc cag cgc cta ggc gaa ctg tct cag 3855 Leu Ala Gln Leu Glu Ala Leu Thr Gln Arg Leu Gly Glu Leu Ser Gln 600 605 610 cag gtg gcc cag ttg cgc gag caa act gag tct gct gtt gcc aca gca 3903 Gln Val Ala Gln Leu Arg Glu Gln Thr Glu Ser Ala Val Ala Thr Ala 615 620 625 630 aag tct aaa taaagattcc caaatcaata aataaaggag atccttgttg 3952 Lys Ser Lys attgtaaaaa caagtgtaat gaatctttat ttgatttttc gcgcgcggta tgccctggac 4012 caccggtctc gatcattgag aactcggtgg atcttttcca ggaccctgta gaggtgggat 4072 tgaatgttta gatacatggg cattaggccg tctcgggggt ggagatagct ccattgaaga 4132 gcctcatgct ccggggtagt gttataaatc acccagtcat aacaaggtcg gagtgcatgg 4192 tgttgcacaa tatcttttag gagcaggcta attgcaaccg ggaggccctt agtgtaggtg 4252 tttacaaatc tgttgagctg ggacgggtgc atccggggtg aaattatatg cattttggac 4312 tggatcttga ggttggcaat gttgccgcct agatcccgtc tcgggttcat attgtgcagg 4372 accaccaaga cagtgtatcc ggtgcacttg ggaaatttat catgcagctt agagggaaaa 4432 gcatgaaaaa atttggagac gcctttgtgt ccgcccagat tctccatgca ctcatccata 4492 atgatagcga tgggaccgtg ggcggcggcg cgggcaaaca cgttccgggg gtctgacaca 4552 tcatagttat gctcttgagt caggtcatca taagccattt taataaactt ggggcggagg 4612 gtgccagatt gggggatgaa agttccctcg ggccccggag catagtttcc ctcacatatt 4672 tgcatttccc aagctttcag ttcagagggg gggatcatgt ccacctgcgg ggctataaaa 4732 aataccgttt ctggagccgg ggtgattaac tgggatgaga gcaaattcct gagcagctga 4792 gacttgccgc acccagtggg accgtaaatg accccgatta cgggttgcag atggtagttt 4852 agggagcggc agctgccgtc ctcccggagc aggggggcca cttcgttcat catttccctt 4912 acatggatat tttcccgcac caagtccatt aggaggcgct ctccccccag ggatagaagc 4972 tcctggagcg aggagaagtt tttcagcggc ttcagcccgt cagccatggg cattttggag 5032 agagtctgtt gcaagagctc gagtcggtcc cagagctcgg tgatgtgttc tatggcatct 5092 cgatccagca gacctcctcg tttcgcgggt ttggacggct cctggagtat ggtatcagac 5152 gatgggcgtc cagcgctgcc agggtccgat ctttccaggg tcgcagcgtc cgagtcaggg 5212 ttgtttccgt cacggtgaag gggtgcgcgc ctggttgggc gcttgcgagg gtgcgcttca 5272 ggctcatcct gctggtcgag aaccgctgcc gatcggcgcc ctgcatgtcg gccaggtagc 5332 agtttaccat gagttcgtaa ttgagcacct cggccgcgtg gcctttggcg cggagcttac 5392 ctttggaagt tttctggcag gcggggcagt acagacactt gagggcatac agtttgggag 5452 cgaggaagat ggattcgggg gagtatgcat ccgcaccgca ggaggcgcag acggtttcgc 5512 attccacgag ccaggtcaga tccggctcat cggggtcaaa aacaagtttt cccccatgtt 5572 ttttgatgcg tttcttacct ttggtctcca tgagttcgtg cccccgctgg gtgacaaaga 5632 ggctgtccgt gtccccgtag accgatttta tgggcctgtc ctcgagcgga gtgcctcggt 5692 cctcttcgta gaggaactcg gaccactctg atacaaaggc gcgcgtccag gccagcacaa 5752 aagaggccac gtgggagggg tagcggtcat tgtcaaccag ggggtccacc ttctccacag 5812 tatgtaaaca catgtccccc tcctccacat ccaggaatgt gattggcttg taagtgtatg 5872 ccacgtgacc aggggtcccc gccggggggg tataaaaggg ggcgggtctc tgctcgtcct 5932 cactgtcttc cggatcgctg tccaggagcg ccagctgttg gggtaggtat tccctctcga 5992 aggcgggcat aacctctgca ctcaggttgt cagtttctag gaacgaggag gatttgatat 6052 tgacagtgcc agccgagatg cctttcataa gactctcgtc catttggtca gaaaatacaa 6112 tctttttgtt gtccagcttg gtggcaaagg atccatagag ggcattggat aagagcttgg 6172 ctatggagcg catggtttgg ttcttttcct tgtcagcgcg ctccttggca gcaatgttga 6232 gctggacata ctcgcgcgcc agacacttcc attcagggaa gatggttgtc agttcatctg 6292 gcacgattct gactcgccaa cctctattat gcagggtgat cagatccaca ctggtggtca 6352 cttcgcctct gaggggctcg ttggtccagc agagtcgacc cccttttctc gaacagaaag 6412 gtgggagggg gtctagcatg agttcatcag gggggtctgc atccatagtg aagattcctg 6472 ggagcagatc cttgtcaaaa tagctgatgg gtgtggggtc atccaaagcc atctgccatt 6532 ctcgagctgc cagcgcgcgc tcataggggt tgagaggggc gccccagggc atggggtggg 6592 tgagtgcaga ggcatacatg ccacagatat catagacata gaggggctct tcgaggatgc 6652 caatgtaggt gggataacag cgcccccctc tgatgcttgc tcgcacatag tcatagagtt 6712 catgcgaggg ggcgagtaga cccgagccca aattagtgcg attgggtttt tcagccctgt 6772 agacgatctg gcgaaagatg gcatgtgaat ttgaagagat ggtgggtctc tgaaagatgt 6832 taaaatgggc atgaggtaga cctacagagt ccctgatgaa gtgggcatat gactcttgca 6892 gcttggccac cagctctgca gtgacaagga catccaaggc gcagtagtca agggtctctt 6952 ggatgatgtc ataacctggt tggtttttct tttcccacag ctcgcggttg agaaggtatt 7012 cttcgcgatc cttccagtac tcttcgaggg gaaacccgtc tttgtctgca cggtaagagc 7072 ccagcatgta gaactgatta actgccttgt agggacagca tcccttctcc acggggagag 7132 agtatgcttg ggcagccttg cgcagtgagg tatgagtgag ggcaaaggtg tccctgacca 7192 tgactttgag gaactggtac ttgaaatcga tgtcatcaca ggccccctgt tcccagagtt 7252 ggaagtccac ccgctttttg taggcggggt tgggcaaagc gaaagtaaca tcattgaaga 7312 gaattttgcc ggccctgggc atgaaattgc gggtgatgcg gaaaggctgg ggcacctctg 7372 ctcggttatt gatcacctga gcggccagga cgatctcatc aaaaccattg atgttgtgtc 7432 ccacaatgta aagttctatg aatcgcgggg tgcccttgac atgaggcagc ttcttgagtt 7492 cttcaaaagt gaggtctgta gggtcagaga gagcatagtg ttcgagggcc cattcgtgca 7552 ggtgagggtt tgcagtgagg aaggaggacc agagatccac tgccagtgct gtttgtaact 7612 ggtcccgata ctggcgaaaa tgttggccga ctgccatctt ttctggggtt atacagtaga 7672 aggttttggg gtcttgctgc cagcgatccc acttgagttt catggcaagg tcgtaggcga 7732 tgttgacgag ccgctcgtcc cccgaaagtt tcatgaccag catgaagggg attagctgct 7792 tgccaaagga ccccatccag gtgtaggttt ccacatcgta ggtgaggaag agcctttctg 7852 tgcgaggatg agagccgatc gggaagaact ggatctcctg ccaccagttg gaggaatggc 7912 tgttgatgtg atggaagtag aactccctgc ggcgcgccga gcattcatgc ttgtgcttgt 7972 acagacggcc gcagtactcg cagcgcttca cgggatgcac ctcatgaatg agttgtacct 8032 ggcttccttt gacgagaaat ttcagtggga agttgaggcc tggcgcttgt acctcgcgct 8092 ctactatgtt atctgcatcg gcctggccat cttctgtctc gatggtggtc atgctgacga 8152 gcccccgcgg gaggcaagtc cagacctcgg cgcgggaggg gcggagctcg aggacgagag 8212 cgcgcaggcc ggagctgtcc agggtcctga gtcgctgcgg agtcaggtta gtaggtagtg 8272 tcagtagatt aacttgcatg atcttttcga gggcatgcgg gaggttcaga tggtacttga 8332 tctccacggg tccgttggtg gagatgtcga tggcttgcag ggtcccgtgc cccttgggcg 8392 ccaccaccgt gcccttgttt ttccttttgg gcggaggagg cggctctgtt gcttcttgca 8452 tgttcagaag cggtggcgag ggcgcgcgcc gggcggtagg ggcggctctg gccccggcgg 8512 catggctggc agaggcacgt cggcgccgcg cgcgggtagg ttctggtact gcgctctgag 8572 aagacttgcg tgcgcgacga cgcggcggtt gacgtcctgg atctgacgcc tctgggtgaa 8632 agctaccgga cccgtgagct tgaacctgaa agagagttca acagaatcaa tttcggtatc 8692 gttgacggcg gcttgcctca ggatctcttg cacgtcgccc gagttgtcct ggtaggcgat 8752 ctcggccatg aactgctcga tttcttcctc ctgaagatct ccgcggcccg ctctctcgac 8812 ggtggcagcg aggtcgttgg agatgcgacc catgagttga gagaatgcat tcatgcccgc 8872 ctcgttccag acgcggctgt agaccacggc cccctcggga tctctcgcgc gcatgaccac 8932 ctgggcgagg ttgagctcca cgtggcgggt gaagaccgca tagttgcata ggcgctggaa 8992 gaggtagttg agtgtggtgg cgatgtgctc ggtgacgaag aaatacatga tccatcgtct 9052 cagcggcatt tcgctgacat cgcccagggc ttccaagcgc tccatggctt cgtagaagtc 9112 cacagcgaag ttgaaaaact gggagttgcg cgcggacacg gtcaactcct cctccagaag 9172 acggatgaga tcggcgatgg tggcgcgcac ctcgcgctca aaagcccccg ggatttcttc 9232 ctcctcctct tcttctatct cttcttccac taacatctct tcttcctctt caggcggggg 9292 cggaggagga gggggcgcgc ggcgacgccg gcggcgcacg ggcagacggt cgatgaatct 9352 ttcaatgacc tctccgcggc ggcggcgcat ggtctcggtg acggcgcggc cgttctccct 9412 gggtctcaga gtgaagacgc ctccgcgcat ctccctgaag tggtgattgg ggggctctcc 9472 gtttggcagg gacagggcgc tgatgatgca ttttatcaat tgccccgtag ggactccgcg 9532 caaggacctg atcgtctgaa gatccacggg atctgaaaac ctttcgacga aagcgtctaa 9592 ccagtcgcaa tcgcaaggta ggctgagcac tgtttcttgc gggcgggggt ggctagatgc 9652 tcggtcgggg ttctctcttc cttctccttc ctcatcatct cgggagggtg agacgatgct 9712 gctggtgatg aaattaaaat aggcagttct gagacggcgg atggtggcga ggagcaccag 9772 gtctttgggc ccggcttgct ggatgcgcag gcgatcggcc attccccaag cattatcctg 9832 gcatcgggcc agatctttat agtagtcttg catgagtcgc tccacgggca cttcttcttc 9892 gcccgctcta ccatgcatgc gcgtgagtcc gaacccgcgc atgggctgga caagtgccag 9952 gtccgctacg accctttcgg cgaggatggc ttgctgcacc tgggtgaggg tggcttggaa 10012 gtcgtcaaag tccacgaagc gatggtaggc cccggtgttg atggtgtagg agcagttggc 10072 catgactgac cagttgactg tctggtgccc cggacgcacg atctcggtgt acttgagtcg 10132 cgagtaggcg cgggtgtcaa agatgtaatc gttgcaggtg cgcaccaggt actggtagcc 10192 gatgagaaag tgcggcggtg gctggcggta gaggggccat cgctctgtag ccggggctcc 10252 gggggcgagg tcttccagca tgaggcggtg gtatccgtag atgtacctgg acatccaggt 10312 gatcccggag gcggtggtgg acgcccgcgg gaactcgcgc actcggttcc agatgttgcg 10372 cagcggcatg aagtagttca tggtaggcac ggtctggcca gtgaggcggg cgcagtcatt 10432 gatgctctat agacacggag aaaacgaaag cgatgagcgg ctcgcctccg tggcctggag 10492 gaacgtgaac gggttgggtc gcggtgtacc ccggttcgag accaaagcca agcgagcaca 10552 actcgggccg gccggagccg cggctaacgt ggtattggcg atcccgtctc gacccagccg 10612 acgaatatcc aggatacgga gtcgagtcgt tttgctgctt gttgcttttt cctggacggg 10672 agccagcgcc gcgtcaagct ttagaacgct cagttcacgg ggccgggagt ggctcgcgcc 10732 cgtagtctgg agaatcaatc gccagggttg cgttgcggtg tgccccggtt cgagccttag 10792 cgcggcccgg atcggccggt ttccgcggca agcgagggtt tggcagcccc gtcatttcta 10852 agaccccgcc agccgacttc tccagtttac gggagcgagc cctctttttt ttttgttttt 10912 gtcgcccag atg cat ccc gtg ctg cga cag atg cgc ccc cag caa cag gtc 10963 Met His Pro Val Leu Arg Gln Met Arg Pro Gln Gln Gln Val 635 640 645 cct tct cag caa cag cag cag cca caa aag gct ctt cct gct cct gct 11011 Pro Ser Gln Gln Gln Gln Gln Pro Gln Lys Ala Leu Pro Ala Pro Ala 650 655 660 cct gca act act gca gtc gca gcc gtg tgc ggc gcg gga cag ccc gcc 11059 Pro Ala Thr Thr Ala Val Ala Ala Val Cys Gly Ala Gly Gln Pro Ala 665 670 675 tat gat ctg gac ttg gaa gag ggc gag gga ctg gcg cgc ctg ggt gca 11107 Tyr Asp Leu Asp Leu Glu Glu Gly Glu Gly Leu Ala Arg Leu Gly Ala 680 685 690 695 cca tcg ccc gag cgg cac ccg cgg gtg caa ctg aaa aag gac tct cgc 11155 Pro Ser Pro Glu Arg His Pro Arg Val Gln Leu Lys Lys Asp Ser Arg 700 705 710 gag gcg tac gtg ccc cag cag aac ctg ttc agg gac agg agc ggc gag 11203 Glu Ala Tyr Val Pro Gln Gln Asn Leu Phe Arg Asp Arg Ser Gly Glu 715 720 725 gag ccc gag gag atg cga gcc tct cgc ttt aac gcg ggt cgc gag ctg 11251 Glu Pro Glu Glu Met Arg Ala Ser Arg Phe Asn Ala Gly Arg Glu Leu 730 735 740 cgc cac ggt ctg gac cga aga cgg gtg ctg cgg gac gag gat ttc gag 11299 Arg His Gly Leu Asp Arg Arg Arg Val Leu Arg Asp Glu Asp Phe Glu 745 750 755 gtc gat gaa gtg aca ggg atc agc ccc gct agg gca cat gtg gcc gcg 11347 Val Asp Glu Val Thr Gly Ile Ser Pro Ala Arg Ala His Val Ala Ala 760 765 770 775 gcc aac ctc gtc tcg gcc tac gag cag acc gtg aag gag gag cgc aac 11395 Ala Asn Leu Val Ser Ala Tyr Glu Gln Thr Val Lys Glu Glu Arg Asn 780 785 790 ttc caa aaa tca ttt aac aac cat gtg cgc acc ctg atc gcc cgt gag 11443 Phe Gln Lys Ser Phe Asn Asn His Val Arg Thr Leu Ile Ala Arg Glu 795 800 805 gaa gtg act ctg ggt ctg atg cac ctg tgg gac ctg atg gaa gct atc 11491 Glu Val Thr Leu Gly Leu Met His Leu Trp Asp Leu Met Glu Ala Ile 810 815 820 acc cag aac ccc act agc aaa ccc ctg acc gct cag ctg ttt ctg gta 11539 Thr Gln Asn Pro Thr Ser Lys Pro Leu Thr Ala Gln Leu Phe Leu Val 825 830 835 gtg caa cat agc agg gac aat gag gca ttc agg gag gcg ctg ctg aac 11587 Val Gln His Ser Arg Asp Asn Glu Ala Phe Arg Glu Ala Leu Leu Asn 840 845 850 855 atc acc gag ccc gag ggg aga tgg ttg tat gat ctg atc aat atc ctg 11635 Ile Thr Glu Pro Glu Gly Arg Trp Leu Tyr Asp Leu Ile Asn Ile Leu 860 865 870 cag agt att ata gtg cag gaa cgt agc ttg ggt ctg gct gag aaa gtg 11683 Gln Ser Ile Ile Val Gln Glu Arg Ser Leu Gly Leu Ala Glu Lys Val 875 880 885 gca gcc atc aac tac tcg gtc ttg agc ctg ggc aag tac tac gct cgc 11731 Ala Ala Ile Asn Tyr Ser Val Leu Ser Leu Gly Lys Tyr Tyr Ala Arg 890 895 900 aag atc tac aag acc ccc tac gtg ccc ata gac aag gag gtg aag ata 11779 Lys Ile Tyr Lys Thr Pro Tyr Val Pro Ile Asp Lys Glu Val Lys Ile 905 910 915 gat ggg ttt tac atg cgc atg act ctc aag gtg ctg act ctc agt gac 11827 Asp Gly Phe Tyr Met Arg Met Thr Leu Lys Val Leu Thr Leu Ser Asp 920 925 930 935 gat ctg ggg gtg tac cgc aac gac agg atg cac cgc gcg gtg agc gcc 11875 Asp Leu Gly Val Tyr Arg Asn Asp Arg Met His Arg Ala Val Ser Ala 940 945 950 agc agg agg cgc gag ctg agc gac aga gaa ctt atg cac agc ttg caa 11923 Ser Arg Arg Arg Glu Leu Ser Asp Arg Glu Leu Met His Ser Leu Gln 955 960 965 aga gct ctg acg ggg gca ggg acc gat ggg gag aac tac ttt gac atg 11971 Arg Ala Leu Thr Gly Ala Gly Thr Asp Gly Glu Asn Tyr Phe Asp Met 970 975 980 ggg gca gac ttg cag tgg caa cct agc cgc agg acc ctg gac gcg gca 12019 Gly Ala Asp Leu Gln Trp Gln Pro Ser Arg Arg Thr Leu Asp Ala Ala 985 990 995 ggg tgt gag ctt cct tac gtg gaa gag gtg gat gaa ggc gag gag 12064 Gly Cys Glu Leu Pro Tyr Val Glu Glu Val Asp Glu Gly Glu Glu 1000 1005 1010 gag gag ggc gag tac ctg gaa gac tgatggcgcg acccgtattt ttgctag 12115 Glu Glu Gly Glu Tyr Leu Glu Asp 1015 1020 atg gaa cag cag gca ccg gac ccc gca atg cgg gcg gcg ctg cag 12160 Met Glu Gln Gln Ala Pro Asp Pro Ala Met Arg Ala Ala Leu Gln 1025 1030 1035 agc cag ccg tcc ggc att aac tcc tcg gac gat tgg acc cag gcc 12205 Ser Gln Pro Ser Gly Ile Asn Ser Ser Asp Asp Trp Thr Gln Ala 1040 1045 1050 atg caa cgc atc atg gcg ctg acg acc cgc aac ccc gaa gcc ttt 12250 Met Gln Arg Ile Met Ala Leu Thr Thr Arg Asn Pro Glu Ala Phe 1055 1060 1065 aga cag caa ccc cag gcc aac cgc ctt tcg gcc atc ctg gag gcc 12295 Arg Gln Gln Pro Gln Ala Asn Arg Leu Ser Ala Ile Leu Glu Ala 1070 1075 1080 gta gtt cct tcc cgc tcc aac ccc acc cac gag aag gtc ctg gcc 12340 Val Val Pro Ser Arg Ser Asn Pro Thr His Glu Lys Val Leu Ala 1085 1090 1095 atc gtg aac gcg ctg gtg gag aac aag gcc atc cgt ccc gat gag 12385 Ile Val Asn Ala Leu Val Glu Asn Lys Ala Ile Arg Pro Asp Glu 1100 1105 1110 gcc ggg ctg gta tac aat gcc ctc ttg gag cgc gtg gcc cgc tac 12430 Ala Gly Leu Val Tyr Asn Ala Leu Leu Glu Arg Val Ala Arg Tyr 1115 1120 1125 aac agc agc aac gtg cag acc aac ctg gac cgg atg gtg acc gat 12475 Asn Ser Ser Asn Val Gln Thr Asn Leu Asp Arg Met Val Thr Asp 1130 1135 1140 gtg cgc gag gcc gtg tct cag cgc gag cgg ttc cag cgc gat gcc 12520 Val Arg Glu Ala Val Ser Gln Arg Glu Arg Phe Gln Arg Asp Ala 1145 1150 1155 aac ttg ggg tcg ctg gtg gcg ctg aac gcc ttt ctc agc acc cag 12565 Asn Leu Gly Ser Leu Val Ala Leu Asn Ala Phe Leu Ser Thr Gln 1160 1165 1170 cct gcc aac gtg ccc cgc ggc cag caa gac tat aca aac ttt cta 12610 Pro Ala Asn Val Pro Arg Gly Gln Gln Asp Tyr Thr Asn Phe Leu 1175 1180 1185 agt gca ctg aga ctc atg gta acc gaa gtc cct cag agc gag gtg 12655 Ser Ala Leu Arg Leu Met Val Thr Glu Val Pro Gln Ser Glu Val 1190 1195 1200 tac cag tcc gga cca gac tat ttc ttc cag acc agc aga cag ggc 12700 Tyr Gln Ser Gly Pro Asp Tyr Phe Phe Gln Thr Ser Arg Gln Gly 1205 1210 1215 ttg cag aca gtg aat ctg agc cag gct ttc aag aac cta aag ggg 12745 Leu Gln Thr Val Asn Leu Ser Gln Ala Phe Lys Asn Leu Lys Gly 1220 1225 1230 ctg tgg gga gtg cac gcc ccg gta gga gat cgt gcg acc gtg tct 12790 Leu Trp Gly Val His Ala Pro Val Gly Asp Arg Ala Thr Val Ser 1235 1240 1245 agc ttg ctg acc ccc aac tcc cgc cta ctg ctg ctg ctg gtt gcc 12835 Ser Leu Leu Thr Pro Asn Ser Arg Leu Leu Leu Leu Leu Val Ala 1250 1255 1260 ccc ttc act gat agc ggt agc atc gac cgc aac tcc tac ttg ggc 12880 Pro Phe Thr Asp Ser Gly Ser Ile Asp Arg Asn Ser Tyr Leu Gly 1265 1270 1275 tat ctg ctg aac ttg tat cgc gag gcc ata gga cag agc cag gtg 12925 Tyr Leu Leu Asn Leu Tyr Arg Glu Ala Ile Gly Gln Ser Gln Val 1280 1285 1290 gac gag cag acc tac caa gaa atc acc caa gtg agc cgc gcc ctg 12970 Asp Glu Gln Thr Tyr Gln Glu Ile Thr Gln Val Ser Arg Ala Leu 1295 1300 1305 ggt cag gaa gat acg ggc agt ttg gaa gcc acc ttg aac ttc ttg 13015 Gly Gln Glu Asp Thr Gly Ser Leu Glu Ala Thr Leu Asn Phe Leu 1310 1315 1320 ctg acc aac cgg tcg cag aag atc cct cct cag tat gcg ctt acc 13060 Leu Thr Asn Arg Ser Gln Lys Ile Pro Pro Gln Tyr Ala Leu Thr 1325 1330 1335 gcg gag gag gag cgg atc ctg aga tat gtc cag cag agc gtg gga 13105 Ala Glu Glu Glu Arg Ile Leu Arg Tyr Val Gln Gln Ser Val Gly 1340 1345 1350 ctg ttc ctg atg caa gag gga gcg acc cct agt gcc gcg ctg gac 13150 Leu Phe Leu Met Gln Glu Gly Ala Thr Pro Ser Ala Ala Leu Asp 1355 1360 1365 atg aca gcg cga aac atg gag ccc agc atg tat gcc agt aac cgg 13195 Met Thr Ala Arg Asn Met Glu Pro Ser Met Tyr Ala Ser Asn Arg 1370 1375 1380 cct ttc atc aac aaa ctg ctg gac tac ctg cac aga gca gcc gct 13240 Pro Phe Ile Asn Lys Leu Leu Asp Tyr Leu His Arg Ala Ala Ala 1385 1390 1395 atg aac tct gat tat ttc acc aat gct atc ctc aac ccc cac tgg 13285 Met Asn Ser Asp Tyr Phe Thr Asn Ala Ile Leu Asn Pro His Trp 1400 1405 1410 ctg ccc ccg cct gga ttt tac acg ggc gag tac gac atg ccc gac 13330 Leu Pro Pro Pro Gly Phe Tyr Thr Gly Glu Tyr Asp Met Pro Asp 1415 1420 1425 ccc aat gac ggg ttc ctg tgg gac gat gtg gac agc agc ata ttc 13375 Pro Asn Asp Gly Phe Leu Trp Asp Asp Val Asp Ser Ser Ile Phe 1430 1435 1440 tcc ccg cct cct ggt tat aac act tgg aag aag gaa ggg ggc gat 13420 Ser Pro Pro Pro Gly Tyr Asn Thr Trp Lys Lys Glu Gly Gly Asp 1445 1450 1455 aga aga cac tct tcc gtg tcg ctg tcc gga tcg agg ggt gct gcc 13465 Arg Arg His Ser Ser Val Ser Leu Ser Gly Ser Arg Gly Ala Ala 1460 1465 1470 gct gcg gtg ccc gag gct gca agt cct ttc cct agc ctg ccc ttt 13510 Ala Ala Val Pro Glu Ala Ala Ser Pro Phe Pro Ser Leu Pro Phe 1475 1480 1485 tct ctg aac agc gtg cgc agc agt gaa ctg ggt aga ata acc cgc 13555 Ser Leu Asn Ser Val Arg Ser Ser Glu Leu Gly Arg Ile Thr Arg 1490 1495 1500 ccg cgc ttg atg ggc gag gat gag tac ttg aac gac tcc ttg ctt 13600 Pro Arg Leu Met Gly Glu Asp Glu Tyr Leu Asn Asp Ser Leu Leu 1505 1510 1515 aga ccc gag agg gaa aag aac ttc ccc aac aat ggg ata gag agc 13645 Arg Pro Glu Arg Glu Lys Asn Phe Pro Asn Asn Gly Ile Glu Ser 1520 1525 1530 ctg gtg gat aag atg agt aga tgg aag acc tat gca cag gat cac 13690 Leu Val Asp Lys Met Ser Arg Trp Lys Thr Tyr Ala Gln Asp His 1535 1540 1545 aaa gac gag cct agg atc ttg ggg gct gcg agc ggg acg acc cgt 13735 Lys Asp Glu Pro Arg Ile Leu Gly Ala Ala Ser Gly Thr Thr Arg 1550 1555 1560 agg cgc cag cgc cat gac aga cag agg ggt ctt gtg tgg gac gat 13780 Arg Arg Gln Arg His Asp Arg Gln Arg Gly Leu Val Trp Asp Asp 1565 1570 1575 gag gac tcg gcc gat gac agc agc gtg ttg gac ttg ggt ggg aga 13825 Glu Asp Ser Ala Asp Asp Ser Ser Val Leu Asp Leu Gly Gly Arg 1580 1585 1590 gga ggg ggc aac ccg ttc gct cat ctg cgc ccg cac ttt ggg cgc 13870 Gly Gly Gly Asn Pro Phe Ala His Leu Arg Pro His Phe Gly Arg 1595 1600 1605 atg ttg taaaagtgaa agtaaaataa aaaggcaact caccaaggcc atggcgacga 13926 Met Leu gcgtgcgttc gttcttttct gttatctgtg tctagt atg atg agg cga gcc gtg 13980 Met Met Arg Arg Ala Val 1610 1615 cta ggc gga gcg gtg gtg tat ccg gag ggt cct cct cct tcg tac 14025 Leu Gly Gly Ala Val Val Tyr Pro Glu Gly Pro Pro Pro Ser Tyr 1620 1625 1630 gag agc gtg atg cag cag cag gcg gcg gcg gtg atg cag ccc tcg 14070 Glu Ser Val Met Gln Gln Gln Ala Ala Ala Val Met Gln Pro Ser 1635 1640 1645 ctg gag gct ccc ttt gta ccc ccg cgg tac ctg gcg cct aca gag 14115 Leu Glu Ala Pro Phe Val Pro Pro Arg Tyr Leu Ala Pro Thr Glu 1650 1655 1660 ggg aga aac agc att cgt tac tcg gag ctg gcg ccc cag tac gat 14160 Gly Arg Asn Ser Ile Arg Tyr Ser Glu Leu Ala Pro Gln Tyr Asp 1665 1670 1675 acc acc agg ttg tat ctg gtg gac aac aag tcg gcg gac atc gcc 14205 Thr Thr Arg Leu Tyr Leu Val Asp Asn Lys Ser Ala Asp Ile Ala 1680 1685 1690 tca ttg aac tat cag aac gac cac agc aac ttc ctg acc acg gtg 14250 Ser Leu Asn Tyr Gln Asn Asp His Ser Asn Phe Leu Thr Thr Val 1695 1700 1705 gtg cag aac aat gac ttt acc ccc acg gag gcc agc acc cag acc 14295 Val Gln Asn Asn Asp Phe Thr Pro Thr Glu Ala Ser Thr Gln Thr 1710 1715 1720 atc aac ttt gac gag cgg tcg cgg tgg ggc ggt cag ctg aag acc 14340 Ile Asn Phe Asp Glu Arg Ser Arg Trp Gly Gly Gln Leu Lys Thr 1725 1730 1735 atc atg cac acc aac atg ccc aac gtg aac gag tac atg ttc agc 14385 Ile Met His Thr Asn Met Pro Asn Val Asn Glu Tyr Met Phe Ser 1740 1745 1750 aac aag ttc aag gct cgg gtg atg gtg tct aga gag gcc tca aag 14430 Asn Lys Phe Lys Ala Arg Val Met Val Ser Arg Glu Ala Ser Lys 1755 1760 1765 att gat tca gag aaa aat gac agg agc aag gac act ctc aag tat 14475 Ile Asp Ser Glu Lys Asn Asp Arg Ser Lys Asp Thr Leu Lys Tyr 1770 1775 1780 gag tgg ttt gag ttc act cta cca gaa ggc aac ttc tca gcc acc 14520 Glu Trp Phe Glu Phe Thr Leu Pro Glu Gly Asn Phe Ser Ala Thr 1785 1790 1795 atg acc atc gac ctg atg aac aat gcc atc att gac aac tac ttg 14565 Met Thr Ile Asp Leu Met Asn Asn Ala Ile Ile Asp Asn Tyr Leu 1800 1805 1810 gcc gtg ggt aga cag aat gga gtg ctg caa agt gac atc ggt gtc 14610 Ala Val Gly Arg Gln Asn Gly Val Leu Gln Ser Asp Ile Gly Val 1815 1820 1825 aag ttt gat acc agg aac ttc agg ctg ggc tgg gac cct gta act 14655 Lys Phe Asp Thr Arg Asn Phe Arg Leu Gly Trp Asp Pro Val Thr 1830 1835 1840 aaa ctt gtt atg cca ggg gtc tac act tat gaa gcc ttt cat cct 14700 Lys Leu Val Met Pro Gly Val Tyr Thr Tyr Glu Ala Phe His Pro 1845 1850 1855 gat att gtt tta cta cct gac tgt ggg gtg gac ttt act gag agc 14745 Asp Ile Val Leu Leu Pro Asp Cys Gly Val Asp Phe Thr Glu Ser 1860 1865 1870 cgc ctt agc aac ttg ctt ggc atc agg aag aga cac cca ttc cag 14790 Arg Leu Ser Asn Leu Leu Gly Ile Arg Lys Arg His Pro Phe Gln 1875 1880 1885 gaa ggc ttc aag ata atg tat gag gat ctt gaa ggg ggt aat atc 14835 Glu Gly Phe Lys Ile Met Tyr Glu Asp Leu Glu Gly Gly Asn Ile 1890 1895 1900 cct gcc ctt cta gat gta gcc gaa tat gaa aaa agc aaa aag gaa 14880 Pro Ala Leu Leu Asp Val Ala Glu Tyr Glu Lys Ser Lys Lys Glu 1905 1910 1915 att gca agc agc act act act act act gca gta aca act gtt gca 14925 Ile Ala Ser Ser Thr Thr Thr Thr Thr Ala Val Thr Thr Val Ala 1920 1925 1930 aga aat gtt gct gat act tca gtt gaa gcg gta gca gtt gcc gta 14970 Arg Asn Val Ala Asp Thr Ser Val Glu Ala Val Ala Val Ala Val 1935 1940 1945 gta gat act att aag gca gaa aat gac agt gcg gtc aga ggc gat 15015 Val Asp Thr Ile Lys Ala Glu Asn Asp Ser Ala Val Arg Gly Asp 1950 1955 1960 aac ttt caa tcg aaa aat gac atg aaa gca tct gag gaa gta acg 15060 Asn Phe Gln Ser Lys Asn Asp Met Lys Ala Ser Glu Glu Val Thr 1965 1970 1975 gtg gtg cct gta agt ccg ccc aca gta act gaa act gaa acc aaa 15105 Val Val Pro Val Ser Pro Pro Thr Val Thr Glu Thr Glu Thr Lys 1980 1985 1990 gaa cca acc att aaa cct cta gaa aag gat acc aag gat cgc agt 15150 Glu Pro Thr Ile Lys Pro Leu Glu Lys Asp Thr Lys Asp Arg Ser 1995 2000 2005 tac aat gtc atc tct ggc acc aat gat act gcc tat cgc agt tgg 15195 Tyr Asn Val Ile Ser Gly Thr Asn Asp Thr Ala Tyr Arg Ser Trp 2010 2015 2020 tac cta gca tac aac tat ggc gac cct gaa aaa gga gtc cgc tcc 15240 Tyr Leu Ala Tyr Asn Tyr Gly Asp Pro Glu Lys Gly Val Arg Ser 2025 2030 2035 tgg aca ctg ctc acc act tca gat gtc acc tgc gga gcg gag caa 15285 Trp Thr Leu Leu Thr Thr Ser Asp Val Thr Cys Gly Ala Glu Gln 2040 2045 2050 gta tat tgg tct ctc cct gac atg atg cag gac ccc gtc acc ttc 15330 Val Tyr Trp Ser Leu Pro Asp Met Met Gln Asp Pro Val Thr Phe 2055 2060 2065 cgc tct acg aga caa gtc agc aac tac ccc gtg gtg ggt gca gag 15375 Arg Ser Thr Arg Gln Val Ser Asn Tyr Pro Val Val Gly Ala Glu 2070 2075 2080 ctc atg ccc gtc ttc tca aag agt ttc tac aac gag caa gcc gtg 15420 Leu Met Pro Val Phe Ser Lys Ser Phe Tyr Asn Glu Gln Ala Val 2085 2090 2095 tac tcc cag cag ctc cgc cag acc acc tcg ctc acg cac gtc ttc 15465 Tyr Ser Gln Gln Leu Arg Gln Thr Thr Ser Leu Thr His Val Phe 2100 2105 2110 aat cgc ttc cct gag aat cag atc ctc atc cgc ccg ccg gcg ccc 15510 Asn Arg Phe Pro Glu Asn Gln Ile Leu Ile Arg Pro Pro Ala Pro 2115 2120 2125 acc att acc acc gtc agt gaa aac gtt cct gct ctc aca gat cac 15555 Thr Ile Thr Thr Val Ser Glu Asn Val Pro Ala Leu Thr Asp His 2130 2135 2140 ggg acc ctg ccg ttg cgc agc agt atc cgg gga gtc cag cgc gtg 15600 Gly Thr Leu Pro Leu Arg Ser Ser Ile Arg Gly Val Gln Arg Val 2145 2150 2155 acc gtt act gac gcc aga cgc cgc acc tgc ccc tac gtc tac aag 15645 Thr Val Thr Asp Ala Arg Arg Arg Thr Cys Pro Tyr Val Tyr Lys 2160 2165 2170 gcc ctg ggc ata gtc gcg ccg cgc gtc ctt tca agc cgc act ttc 15690 Ala Leu Gly Ile Val Ala Pro Arg Val Leu Ser Ser Arg Thr Phe 2175 2180 2185 taaaaa atg tcc att ctc atc tca ccc agt aat aac acc ggt tgg ggg 15738 Met Ser Ile Leu Ile Ser Pro Ser Asn Asn Thr Gly Trp Gly 2190 2195 ctg cgc aca ccc acc agg atg tac gga ggc gct cgc aaa cgg tct 15783 Leu Arg Thr Pro Thr Arg Met Tyr Gly Gly Ala Arg Lys Arg Ser 2200 2205 2210 acc cag cac cct gtg cgt gtg cgc ggg cat ttc cgc gct ccc tgg 15828 Thr Gln His Pro Val Arg Val Arg Gly His Phe Arg Ala Pro Trp 2215 2220 2225 ggc gcc ctc aag ggc cgt gct cgc act agg acc acc gtc gat gat 15873 Gly Ala Leu Lys Gly Arg Ala Arg Thr Arg Thr Thr Val Asp Asp 2230 2235 2240 gtg atc gac cag gtg gtt gca gat gct cgt aat tat act cct gct 15918 Val Ile Asp Gln Val Val Ala Asp Ala Arg Asn Tyr Thr Pro Ala 2245 2250 2255 gca cct gca tct act gtg gat gca gtt att gac agc gtt gtg gct 15963 Ala Pro Ala Ser Thr Val Asp Ala Val Ile Asp Ser Val Val Ala 2260 2265 2270 gac gct cgc gac tat gct cgc agg aag agc agg cga aga cgc atc 16008 Asp Ala Arg Asp Tyr Ala Arg Arg Lys Ser Arg Arg Arg Arg Ile 2275 2280 2285 gcc agg cgc cac cga gct acc ccc gcc atg cga gct gca aga gct 16053 Ala Arg Arg His Arg Ala Thr Pro Ala Met Arg Ala Ala Arg Ala 2290 2295 2300 ctg ctg cgg aga gcc aaa cgc gtg ggg cga aga gcc atg ctt aga 16098 Leu Leu Arg Arg Ala Lys Arg Val Gly Arg Arg Ala Met Leu Arg 2305 2310 2315 gcg gcc aga cgc gcg gct tca ggt gcc agc gca ggc agg tcc cgc 16143 Ala Ala Arg Arg Ala Ala Ser Gly Ala Ser Ala Gly Arg Ser Arg 2320 2325 2330 agg cgc gcg gcc acg gcg gca gca gcg gcc atc gcc aac atg gcc 16188 Arg Arg Ala Ala Thr Ala Ala Ala Ala Ala Ile Ala Asn Met Ala 2335 2340 2345 caa ccg cga aga ggc aat gtg tac tgg gtg cgc gat gcc act acc 16233 Gln Pro Arg Arg Gly Asn Val Tyr Trp Val Arg Asp Ala Thr Thr 2350 2355 2360 ggc cag cgc gtg ccc gtg cgc acc cgt ccc cct cgc act tagaagatac 16282 Gly Gln Arg Val Pro Val Arg Thr Arg Pro Pro Arg Thr 2365 2370 2375 tgagcagtct ccgatgttgt gtcccagcgg cgagg atg tcc aag cgc aaa tac 16335 Met Ser Lys Arg Lys Tyr 2380 aag gaa gag atg ctc cag gtc atc gcg cct gaa atc tac ggt cca 16380 Lys Glu Glu Met Leu Gln Val Ile Ala Pro Glu Ile Tyr Gly Pro 2385 2390 2395 ccg gtg aag gat gaa aaa aag ccc cgc aaa atc aag cgg gtc aaa 16425 Pro Val Lys Asp Glu Lys Lys Pro Arg Lys Ile Lys Arg Val Lys 2400 2405 2410 aag gac aaa aag gaa gaa gat ggc gat gat ggg ctg gtg gag ttt 16470 Lys Asp Lys Lys Glu Glu Asp Gly Asp Asp Gly Leu Val Glu Phe 2415 2420 2425 gtg cgc gag ttc gct cca agg cgg cgc gta cag tgg cgc ggg cgc 16515 Val Arg Glu Phe Ala Pro Arg Arg Arg Val Gln Trp Arg Gly Arg 2430 2435 2440 agg gtg cgg ccg gtg ctg agg ccc gga acc acg gtg gtc ttc acg 16560 Arg Val Arg Pro Val Leu Arg Pro Gly Thr Thr Val Val Phe Thr 2445 2450 2455 ccc ggc gaa cgc tcc agc act act ttt aaa cgc tcc tat gat gag 16605 Pro Gly Glu Arg Ser Ser Thr Thr Phe Lys Arg Ser Tyr Asp Glu 2460 2465 2470 gtg tac ggg gat gat gat att ctg gag cag gca gcc gac cgc ctg 16650 Val Tyr Gly Asp Asp Asp Ile Leu Glu Gln Ala Ala Asp Arg Leu 2475 2480 2485 ggc gag ttt gct tat ggc aaa cgc agc cgc tcc agt ccc aag gat 16695 Gly Glu Phe Ala Tyr Gly Lys Arg Ser Arg Ser Ser Pro Lys Asp 2490 2495 2500 gag gcg gtg tcc atc ccc ttg gat cat gga aat ccc acc cca agt 16740 Glu Ala Val Ser Ile Pro Leu Asp His Gly Asn Pro Thr Pro Ser 2505 2510 2515 cta aaa cca gtc acc ctg cag caa gtg cta ccc gtg cct cca cgg 16785 Leu Lys Pro Val Thr Leu Gln Gln Val Leu Pro Val Pro Pro Arg 2520 2525 2530 aga ggt gtc aag cga gag ggc gag gat ctg tat ccc acc atg caa 16830 Arg Gly Val Lys Arg Glu Gly Glu Asp Leu Tyr Pro Thr Met Gln 2535 2540 2545 ctg atg gtg ccc aag cgc cag aag ctg gag gac gtg ctg gag aaa 16875 Leu Met Val Pro Lys Arg Gln Lys Leu Glu Asp Val Leu Glu Lys 2550 2555 2560 atg aaa gtg gat ccc gat atc cag cct gaa gtt aaa gtc aga ccc 16920 Met Lys Val Asp Pro Asp Ile Gln Pro Glu Val Lys Val Arg Pro 2565 2570 2575 atc aag cag gtg gcg ccc ggt ctg gga gta caa acc gtg gac atc 16965 Ile Lys Gln Val Ala Pro Gly Leu Gly Val Gln Thr Val Asp Ile 2580 2585 2590 aag att ccc acc gag tcc atg gaa gtc cag act gaa cct gca aag 17010 Lys Ile Pro Thr Glu Ser Met Glu Val Gln Thr Glu Pro Ala Lys 2595 2600 2605 cct aca gcc acc tcc att gag gtg cag aca gat cca tgg atg ccc 17055 Pro Thr Ala Thr Ser Ile Glu Val Gln Thr Asp Pro Trp Met Pro 2610 2615 2620 gcg ccc att gca acc acc gcc agt acc gct cga aga cca cgg cga 17100 Ala Pro Ile Ala Thr Thr Ala Ser Thr Ala Arg Arg Pro Arg Arg 2625 2630 2635 aag tat ggt cct gcg agt ctg ctg atg ccc aac tat gct ctg cac 17145 Lys Tyr Gly Pro Ala Ser Leu Leu Met Pro Asn Tyr Ala Leu His 2640 2645 2650 cca tcc att att cca act cct ggt tac cga ggc act cgc tac tac 17190 Pro Ser Ile Ile Pro Thr Pro Gly Tyr Arg Gly Thr Arg Tyr Tyr 2655 2660 2665 cgc agc cgg agc acc act tcc cgc cgc cgc aaa aca cct gca agc 17235 Arg Ser Arg Ser Thr Thr Ser Arg Arg Arg Lys Thr Pro Ala Ser 2670 2675 2680 cgc agc cgc cgt cgc cgc cgc cgc acc gcc agc aaa ctg act ccc 17280 Arg Ser Arg Arg Arg Arg Arg Arg Thr Ala Ser Lys Leu Thr Pro 2685 2690 2695 gcc gct ttg gtg cgg agg gtg tat cgc gat ggc cgc gca gag ccc 17325 Ala Ala Leu Val Arg Arg Val Tyr Arg Asp Gly Arg Ala Glu Pro 2700 2705 2710 ctg atg ctg ccg cgc gca cgc tac cat cca agc atc acc act 17367 Leu Met Leu Pro Arg Ala Arg Tyr His Pro Ser Ile Thr Thr 2715 2720 2725 taatgactgt tgccactgcc tccttgcaga t atg gcc ctc act tgc cgc ctt 17419 Met Ala Leu Thr Cys Arg Leu 2730 cgc gtc ccc att act ggc tac cga gga aga aac tcg cgc cgt aga 17464 Arg Val Pro Ile Thr Gly Tyr Arg Gly Arg Asn Ser Arg Arg Arg 2735 2740 2745 agg atg ttg ggg cgc ggg atg cgt cgc cac agg cgg cgg cgc gct 17509 Arg Met Leu Gly Arg Gly Met Arg Arg His Arg Arg Arg Arg Ala 2750 2755 2760 atc agc aag agg ctg ggg ggt ggc ttt ctg acc gct ttg att ccc 17554 Ile Ser Lys Arg Leu Gly Gly Gly Phe Leu Thr Ala Leu Ile Pro 2765 2770 2775 att atc gcc gcg gcg att ggg gca gta cca ggc ata gct tcc gtg 17599 Ile Ile Ala Ala Ala Ile Gly Ala Val Pro Gly Ile Ala Ser Val 2780 2785 2790 gcg gtt cag gcc tcg cag cgc cac tgacattgga aaaacttata 17643 Ala Val Gln Ala Ser Gln Arg His 2795 2800 aataaaatag aatggactct gacgctcctg gtcctgtgac tatgtttttg tagag atg 17701 Met gaa gac atc aat ttt tca tcc ctg gct ccg cga cac ggc acg agg 17746 Glu Asp Ile Asn Phe Ser Ser Leu Ala Pro Arg His Gly Thr Arg 2805 2810 2815 ccg tac atg ggc acc tgg agc gac atc ggc acc agc caa ctg aac 17791 Pro Tyr Met Gly Thr Trp Ser Asp Ile Gly Thr Ser Gln Leu Asn 2820 2825 2830 ggg ggc gcc ttc aat tgg agc agt atc tgg agc ggg ctt aaa aat 17836 Gly Gly Ala Phe Asn Trp Ser Ser Ile Trp Ser Gly Leu Lys Asn 2835 2840 2845 ttt ggc tct acc ata aaa acc tat ggg aac aaa gct tgg aac agc 17881 Phe Gly Ser Thr Ile Lys Thr Tyr Gly Asn Lys Ala Trp Asn Ser 2850 2855 2860 agc aca ggg cag gcg ctg agg aat aag ctt aaa gag cag aac ttc 17926 Ser Thr Gly Gln Ala Leu Arg Asn Lys Leu Lys Glu Gln Asn Phe 2865 2870 2875 caa cag aag gtg gtc gat ggg atc gcc tct ggc atc aat ggg gtg 17971 Gln Gln Lys Val Val Asp Gly Ile Ala Ser Gly Ile Asn Gly Val 2880 2885 2890 gtg gat ctg gcc aac cag gcc gtg cag aaa cag ata aac agc cgc 18016 Val Asp Leu Ala Asn Gln Ala Val Gln Lys Gln Ile Asn Ser Arg 2895 2900 2905 ctg gac gcg ccg cct gca gcc cct ggc gaa atg gaa gtg gag gaa 18061 Leu Asp Ala Pro Pro Ala Ala Pro Gly Glu Met Glu Val Glu Glu 2910 2915 2920 gag ctc cct ccc ctg gaa aag cgg gga gac aag cgc ccg cgt ccc 18106 Glu Leu Pro Pro Leu Glu Lys Arg Gly Asp Lys Arg Pro Arg Pro 2925 2930 2935 gat atg gag gag acg ctg gtg acg cgc gga gac gag ccg cct cca 18151 Asp Met Glu Glu Thr Leu Val Thr Arg Gly Asp Glu Pro Pro Pro 2940 2945 2950 tac gag gag gca ata aag ctt gga atg ccc act acc agg cct ata 18196 Tyr Glu Glu Ala Ile Lys Leu Gly Met Pro Thr Thr Arg Pro Ile 2955 2960 2965 gct ccc atg gcc acc ggg gta atg aaa cct tct cag tcg cat cga 18241 Ala Pro Met Ala Thr Gly Val Met Lys Pro Ser Gln Ser His Arg 2970 2975 2980 ccc gcc acc ttg gac ttg cct cct tcc cct gct gct gtc gcg tcc 18286 Pro Ala Thr Leu Asp Leu Pro Pro Ser Pro Ala Ala Val Ala Ser 2985 2990 2995 gct cca aag cct gtc gct acc ccg aag ccc acc acc gta cag ccc 18331 Ala Pro Lys Pro Val Ala Thr Pro Lys Pro Thr Thr Val Gln Pro 3000 3005 3010 gtc gcc gta gcc aga ccg cgt ccc ggg ggc act ccg cgc ccg aat 18376 Val Ala Val Ala Arg Pro Arg Pro Gly Gly Thr Pro Arg Pro Asn 3015 3020 3025 gca aac tgg cag agt act ctg aac agc atc gtg ggt ctg ggc gtg 18421 Ala Asn Trp Gln Ser Thr Leu Asn Ser Ile Val Gly Leu Gly Val 3030 3035 3040 cag agt gta aag cgc cgt cgc tgc tat taattaaata tggagtagcg 18468 Gln Ser Val Lys Arg Arg Arg Cys Tyr 3045 3050 cttaacttgc ttgtctgtgt gtatgtgtca tcaccacgcc gccgcagcag cagcagagga 18528 gaaaggaaga ggtcgcgcga cgaggctgag ttgctttcaa g atg gcc acc cca 18581 Met Ala Thr Pro 3055 tcg atg ttg ccc cag tgg gca tac atg cac atc gcc gga cag gat 18626 Ser Met Leu Pro Gln Trp Ala Tyr Met His Ile Ala Gly Gln Asp 3060 3065 3070 gct tcg gaa tac ctg agt ccg ggt ctg gtg cag ttc gcc cgt gcc 18671 Ala Ser Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala Arg Ala 3075 3080 3085 aca gac acc tac ttc aat ctg ggg aac aag ttt agg aac ccc acc 18716 Thr Asp Thr Tyr Phe Asn Leu Gly Asn Lys Phe Arg Asn Pro Thr 3090 3095 3100 gtg gcc ccc acc cac gat gtg acc acc gac cga agc cag cgg ctg 18761 Val Ala Pro Thr His Asp Val Thr Thr Asp Arg Ser Gln Arg Leu 3105 3110 3115 atg ctg cgc ttt gtg ccc gtt gat cgg gag gac aat acc tac tct 18806 Met Leu Arg Phe Val Pro Val Asp Arg Glu Asp Asn Thr Tyr Ser 3120 3125 3130 tac aaa gtt cgc tac aca ctg gct gtg ggc gac aac aga gtg ctg 18851 Tyr Lys Val Arg Tyr Thr Leu Ala Val Gly Asp Asn Arg Val Leu 3135 3140 3145 gat atg gcc agc acc ttc ttt gac atc agg ggc gtg ctt gac aga 18896 Asp Met Ala Ser Thr Phe Phe Asp Ile Arg Gly Val Leu Asp Arg 3150 3155 3160 ggt ccc agt ttc aag cca tac tct ggc aca gct tac aac tcc ctg 18941 Gly Pro Ser Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser Leu 3165 3170 3175 gct cct aaa ggc gcc ccc aat aca tct cag tgg gtt gat gaa gga 18986 Ala Pro Lys Gly Ala Pro Asn Thr Ser Gln Trp Val Asp Glu Gly 3180 3185 3190 aag aaa att aca gac aac ggt gtg gaa gca gct gat gac aat gca 19031 Lys Lys Ile Thr Asp Asn Gly Val Glu Ala Ala Asp Asp Asn Ala 3195 3200 3205 aaa gct act tac act ttt ggc aat gct cca gtg aaa gcg gag gat 19076 Lys Ala Thr Tyr Thr Phe Gly Asn Ala Pro Val Lys Ala Glu Asp 3210 3215 3220 gac att aca aaa gat ggt tta cca gta gca gta gaa gtt act ggc 19121 Asp Ile Thr Lys Asp Gly Leu Pro Val Ala Val Glu Val Thr Gly 3225 3230 3235 gaa gat gat gaa act aaa cca att tat gca gat aag ttg tat cag 19166 Glu Asp Asp Glu Thr Lys Pro Ile Tyr Ala Asp Lys Leu Tyr Gln 3240 3245 3250 cca gaa cca cag gta gga gag gaa aca tgg act gat aca gat gcg 19211 Pro Glu Pro Gln Val Gly Glu Glu Thr Trp Thr Asp Thr Asp Ala 3255 3260 3265 acc act gag aag tat ggc ggt aga gca ctt aag cct gac act aaa 19256 Thr Thr Glu Lys Tyr Gly Gly Arg Ala Leu Lys Pro Asp Thr Lys 3270 3275 3280 atg aaa cca tgt tac gga tct ttt gct aaa cca acc aat act aaa 19301 Met Lys Pro Cys Tyr Gly Ser Phe Ala Lys Pro Thr Asn Thr Lys 3285 3290 3295 ggt ggt cag gca aaa gta aaa aag aca gaa gaa gaa aca gtt gac 19346 Gly Gly Gln Ala Lys Val Lys Lys Thr Glu Glu Glu Thr Val Asp 3300 3305 3310 cct aca aaa gtt caa tat gac att gat atg aac ttc ttt gaa gaa 19391 Pro Thr Lys Val Gln Tyr Asp Ile Asp Met Asn Phe Phe Glu Glu 3315 3320 3325 aga tct cag aaa aat ggc agt ccc aaa att gtg atg tat gca gaa 19436 Arg Ser Gln Lys Asn Gly Ser Pro Lys Ile Val Met Tyr Ala Glu 3330 3335 3340 aat gta gat ttg gaa act cca gat act cat gtg gtg tac aaa cct 19481 Asn Val Asp Leu Glu Thr Pro Asp Thr His Val Val Tyr Lys Pro 3345 3350 3355 ggt act tca gaa gac agt tca cat gct aat ctg ggt caa cag tct 19526 Gly Thr Ser Glu Asp Ser Ser His Ala Asn Leu Gly Gln Gln Ser 3360 3365 3370 atg ccc aat aga ccc aac tac att ggc ttt agg gat aat ttt atc 19571 Met Pro Asn Arg Pro Asn Tyr Ile Gly Phe Arg Asp Asn Phe Ile 3375 3380 3385 ggc ctt atg tac tac aac agc act ggg aac atg gga gtg ctg gct 19616 Gly Leu Met Tyr Tyr Asn Ser Thr Gly Asn Met Gly Val Leu Ala 3390 3395 3400 ggt caa gca tct cag ttg aat gcg gtg gtt gac ttg caa gac aga 19661 Gly Gln Ala Ser Gln Leu Asn Ala Val Val Asp Leu Gln Asp Arg 3405 3410 3415 aac aca gaa ctg tct tac caa tta ttg ctt gac tct ctg ggc gac 19706 Asn Thr Glu Leu Ser Tyr Gln Leu Leu Leu Asp Ser Leu Gly Asp 3420 3425 3430 agg acc aga tac ttt agc atg tgg aat cag gcg gta gat agc tat 19751 Arg Thr Arg Tyr Phe Ser Met Trp Asn Gln Ala Val Asp Ser Tyr 3435 3440 3445 gat cct gat gtg cgc att att gaa aat cat gga gtg gaa gac gag 19796 Asp Pro Asp Val Arg Ile Ile Glu Asn His Gly Val Glu Asp Glu 3450 3455 3460 ctt ccc aac tac tgc ttt cca tta gat gga gtg ggc cca aga aca 19841 Leu Pro Asn Tyr Cys Phe Pro Leu Asp Gly Val Gly Pro Arg Thr 3465 3470 3475 gtt agc tac aaa caa atg gaa cca aac ggg aca gac gca gca gat 19886 Val Ser Tyr Lys Gln Met Glu Pro Asn Gly Thr Asp Ala Ala Asp 3480 3485 3490 gct aat ggt tgg aaa aat gtt act cca act gga atc agt gaa att 19931 Ala Asn Gly Trp Lys Asn Val Thr Pro Thr Gly Ile Ser Glu Ile 3495 3500 3505 gct aaa ggc aat cct ttc gcc atg gaa att aat ctc caa gcc aat 19976 Ala Lys Gly Asn Pro Phe Ala Met Glu Ile Asn Leu Gln Ala Asn 3510 3515 3520 cta tgg aga agt ttc ctt tat tca aat gtg gcc ctg tat cta cca 20021 Leu Trp Arg Ser Phe Leu Tyr Ser Asn Val Ala Leu Tyr Leu Pro 3525 3530 3535 gat tcc tac aaa tac acc ccg gcc aat gtt act ctt ccc acc aac 20066 Asp Ser Tyr Lys Tyr Thr Pro Ala Asn Val Thr Leu Pro Thr Asn 3540 3545 3550 acc aac act tat gac tac ctg aac gga cgg gtg gtt ccc cca tct 20111 Thr Asn Thr Tyr Asp Tyr Leu Asn Gly Arg Val Val Pro Pro Ser 3555 3560 3565 ctg gtg gat act tat gta aac att gga gcc aga tgg tct ctt gat 20156 Leu Val Asp Thr Tyr Val Asn Ile Gly Ala Arg Trp Ser Leu Asp 3570 3575 3580 gcc atg gac aat gtt aat cca ttt aac cac cac cgc aat gct gga 20201 Ala Met Asp Asn Val Asn Pro Phe Asn His His Arg Asn Ala Gly 3585 3590 3595 ctg cgc tac cgg tcc atg ctt ttg ggc aat ggt cgc tat gtt cca 20246 Leu Arg Tyr Arg Ser Met Leu Leu Gly Asn Gly Arg Tyr Val Pro 3600 3605 3610 ttc cac ata caa gtg cct cag aaa ttc ttt gct atc aag aac ctg 20291 Phe His Ile Gln Val Pro Gln Lys Phe Phe Ala Ile Lys Asn Leu 3615 3620 3625 ttg ctt ctc cct ggc tcc tac acc tat gag tgg aac ttc aga aag 20336 Leu Leu Leu Pro Gly Ser Tyr Thr Tyr Glu Trp Asn Phe Arg Lys 3630 3635 3640 gat gtg aac atg gtc ctg cag agt tcc ctt ggt aat gat ctc aga 20381 Asp Val Asn Met Val Leu Gln Ser Ser Leu Gly Asn Asp Leu Arg 3645 3650 3655 act gat gga gcc agc atc agt ttt acc agc atc aac ctc tat gcc 20426 Thr Asp Gly Ala Ser Ile Ser Phe Thr Ser Ile Asn Leu Tyr Ala 3660 3665 3670 acc ttc ttc ccc atg gct cac aac act gct tcc acc ctt gaa gcc 20471 Thr Phe Phe Pro Met Ala His Asn Thr Ala Ser Thr Leu Glu Ala 3675 3680 3685 atg ctg cgc aat gac aca aat gac cag tca ttc aat gac tat ctc 20516 Met Leu Arg Asn Asp Thr Asn Asp Gln Ser Phe Asn Asp Tyr Leu 3690 3695 3700 tct gca gct aac atg ctc tac cct att cca gcc aat gcc acc aac 20561 Ser Ala Ala Asn Met Leu Tyr Pro Ile Pro Ala Asn Ala Thr Asn 3705 3710 3715 att ccc att tcc att cca tct cgc aac tgg gct gcc ttc agg ggc 20606 Ile Pro Ile Ser Ile Pro Ser Arg Asn Trp Ala Ala Phe Arg Gly 3720 3725 3730 tgg tcc ttc acc aga ctc aaa acc aag gaa act ccc tct ctg gga 20651 Trp Ser Phe Thr Arg Leu Lys Thr Lys Glu Thr Pro Ser Leu Gly 3735 3740 3745 tca ggc ttt gat ccc tac ttt gtt tac tct ggc tcc att ccc tac 20696 Ser Gly Phe Asp Pro Tyr Phe Val Tyr Ser Gly Ser Ile Pro Tyr 3750 3755 3760 ctg gat ggt acc ttc tac ctg aac cac act ttc aag aag gtg tcc 20741 Leu Asp Gly Thr Phe Tyr Leu Asn His Thr Phe Lys Lys Val Ser 3765 3770 3775 atc atg ttt gac tcc tca gtc agc tgg cca ggc aat gac aga ttg 20786 Ile Met Phe Asp Ser Ser Val Ser Trp Pro Gly Asn Asp Arg Leu 3780 3785 3790 cta act cca aat gag ttt gaa atc aag cgc act gtg gat ggg gaa 20831 Leu Thr Pro Asn Glu Phe Glu Ile Lys Arg Thr Val Asp Gly Glu 3795 3800 3805 ggg tac aat gtg gct caa tgc aac atg acc aag gat tgg ttc ctg 20876 Gly Tyr Asn Val Ala Gln Cys Asn Met Thr Lys Asp Trp Phe Leu 3810 3815 3820 gtt cag atg ctt gcc aac tat aac att ggc tac cag ggc ttc tac 20921 Val Gln Met Leu Ala Asn Tyr Asn Ile Gly Tyr Gln Gly Phe Tyr 3825 3830 3835 atc cca gag ggg tac aag gat cgc atg tac tcc ttc ttc aga aac 20966 Ile Pro Glu Gly Tyr Lys Asp Arg Met Tyr Ser Phe Phe Arg Asn 3840 3845 3850 ttc cag ccc atg agc aga cag gtg gtc gat gaa att aac tac aag 21011 Phe Gln Pro Met Ser Arg Gln Val Val Asp Glu Ile Asn Tyr Lys 3855 3860 3865 gac tac aaa gcc gtt gct gtt ccc tac cag cac aac aac tct ggc 21056 Asp Tyr Lys Ala Val Ala Val Pro Tyr Gln His Asn Asn Ser Gly 3870 3875 3880 ttt gtg ggt tac atg gct cct acc atg cga cag ggg caa gct tat 21101 Phe Val Gly Tyr Met Ala Pro Thr Met Arg Gln Gly Gln Ala Tyr 3885 3890 3895 cca gct aac tat ccc tac cca cta atc gga acc act gct gtt aag 21146 Pro Ala Asn Tyr Pro Tyr Pro Leu Ile Gly Thr Thr Ala Val Lys 3900 3905 3910 agt gtt acc cag aaa aag ttc ctg tgc gac agg acc atg tgg cgc 21191 Ser Val Thr Gln Lys Lys Phe Leu Cys Asp Arg Thr Met Trp Arg 3915 3920 3925 atc ccc ttc tcc agc aac ttc atg tcc atg ggt gcc cta acc gac 21236 Ile Pro Phe Ser Ser Asn Phe Met Ser Met Gly Ala Leu Thr Asp 3930 3935 3940 ctg ggg cag aac atg ctt tat gcc aac tca gcc cat gcg ctg gac 21281 Leu Gly Gln Asn Met Leu Tyr Ala Asn Ser Ala His Ala Leu Asp 3945 3950 3955 atg act ttt gag gtg gat ccc atg gat gag ccc aca ctg ctt tat 21326 Met Thr Phe Glu Val Asp Pro Met Asp Glu Pro Thr Leu Leu Tyr 3960 3965 3970 ctt ctt ttt gaa gtc ttc gac gtg gtc aga gtg cac cag cca cac 21371 Leu Leu Phe Glu Val Phe Asp Val Val Arg Val His Gln Pro His 3975 3980 3985 cgc ggc gtc atc gag gct gtc tac ctg cgt act cca ttc tca gct 21416 Arg Gly Val Ile Glu Ala Val Tyr Leu Arg Thr Pro Phe Ser Ala 3990 3995 4000 ggt aac gcc acc aca taagaagcct cttgcttctt gcaagcagcc atg gcc tgt 21470 Gly Asn Ala Thr Thr Met Ala Cys 4005 ggg tcc ggc aac gga tcc agc gag caa gag ctc agg gcc att gct 21515 Gly Ser Gly Asn Gly Ser Ser Glu Gln Glu Leu Arg Ala Ile Ala 4010 4015 4020 aga gac ctg ggc tgc gga ccc tat ttc ctg gga acc ttt gac aag 21560 Arg Asp Leu Gly Cys Gly Pro Tyr Phe Leu Gly Thr Phe Asp Lys 4025 4030 4035 cgt ttc ccg ggg ttc atg gcc ccc gac aag ctc gcc tgc gcc att 21605 Arg Phe Pro Gly Phe Met Ala Pro Asp Lys Leu Ala Cys Ala Ile 4040 4045 4050 gtt aac acg gcc ggt cgc gag acg ggg gga gag cac tgg ctg gct 21650 Val Asn Thr Ala Gly Arg Glu Thr Gly Gly Glu His Trp Leu Ala 4055 4060 4065 ttt ggt tgg aac ccg cgc tcc aac acc tgc tac ctt ttt gat cct 21695 Phe Gly Trp Asn Pro Arg Ser Asn Thr Cys Tyr Leu Phe Asp Pro 4070 4075 4080 ttt ggc ttc tcg gat gag cgc ctc aag caa atc tac cag ttt gag 21740 Phe Gly Phe Ser Asp Glu Arg Leu Lys Gln Ile Tyr Gln Phe Glu 4085 4090 4095 tat gag ggt ctc ctg cgc cgc agt gcc ctg gct acc aag gat cgc 21785 Tyr Glu Gly Leu Leu Arg Arg Ser Ala Leu Ala Thr Lys Asp Arg 4100 4105 4110 tgt atc acc ctg gaa aag tcc acc cag acc gtg cag ggc ccg cgc 21830 Cys Ile Thr Leu Glu Lys Ser Thr Gln Thr Val Gln Gly Pro Arg 4115 4120 4125 tcc gca gcc tgt gga ctt ttt tgc tgc atg ttc ctc cac gct ttt 21875 Ser Ala Ala Cys Gly Leu Phe Cys Cys Met Phe Leu His Ala Phe 4130 4135 4140 gtg cac tgg ccc gac cgc ccc atg gac gga aac ccc acc atg aag 21920 Val His Trp Pro Asp Arg Pro Met Asp Gly Asn Pro Thr Met Lys 4145 4150 4155 ttg ttg act ggg gtg ccc aac agc atg ctc caa tca ccc caa gtc 21965 Leu Leu Thr Gly Val Pro Asn Ser Met Leu Gln Ser Pro Gln Val 4160 4165 4170 cag ccc acc ctg cgc cac aac cag gag gcg ctc tac cgc ttc ctc 22010 Gln Pro Thr Leu Arg His Asn Gln Glu Ala Leu Tyr Arg Phe Leu 4175 4180 4185 aat acc cac tca tct tac ttt cgt tct cac cgc gcg cgc atc gaa 22055 Asn Thr His Ser Ser Tyr Phe Arg Ser His Arg Ala Arg Ile Glu 4190 4195 4200 aag gct acc gcg ttt gac cgt atg gat atg caa taataagtca 22098 Lys Ala Thr Ala Phe Asp Arg Met Asp Met Gln 4205 4210 4215 tgtaaaccgt gttcaaataa acagcacttt attttttaca tgcactgtgg ctctgggttg 22158 ctcattcatt catcattcac tcagaagtcg aaggggttct ggcgggaatc agcatgaccc 22218 gctggcaggg atacgttgcg gaactggaac ctgttctgcc acttgaactc ggggatcacc 22278 agtttgggaa ctaggatctc ggggaaggtg tcttgccaca gctttctggt cagttgcaga 22338 gcgccgagca ggtcaggagc agagatcttg aaatcacagt tggggccagc attctgggca 22398 cgggagttgc ggtacactgg gttgcagcac tggaacacca tcagggcggg gtgtctcacg 22458 ctcgccagca cggtcgggtc actgatggta gacacatcca agtcttcagc attggccatt 22518 ccaaaggggg tcatcttaca tgtctgcctg cccatcacgg gagcgcagcc gggcttgtgg 22578 ttgcaatcgc agcgaatggg gatcagcatc atcctggcct ggtcgggggt tatccctgga 22638 tacaccgcct tcataaaggc ttcgtactgc ttgaaagctt cctgggcctt gcttccctcg 22698 gtgtagaaca tcccacagga cttgctggaa aattgattag tagcacagtt ggcatcattc 22758 acacagcagc gggcatcgtt gttggccagc tggaccacat tcctgcccca gcggttctgg 22818 gtgatcttgg ctcgatctgg gttctccttc atcgcgcgct gcccgttctc gctcgccaca 22878 tccatctcga tgatgtgatc cttctggatc atgatagtac catgcaggca tttcaccttg 22938 ccttcataat cggtgcagcc atgagcccac agagcgcacc cggtgcactc ccaattgttg 22998 tgggcgatct cagaataaga atgcaccaat ccctgcatga atcttcccat cattgtagtc 23058 agggtcttta tgctggtaaa tgtcagcggg atgccacggt gctcctcgtt cacatactgg 23118 tggcagatac gcctgtactg ctcctgctgc tcgggcatca gcttgaaaga ggttctcagg 23178 tcattatcca gcctgtacct ctccatcagt acggccatta cttccatgcc cttctcccag 23238 gcagagacca ggggcaggct catgggattc ctaacagcaa gagcagctcc tttagccaga 23298 gggtcattct tgtcaatctt ctcaacactt ctcttgccat ccttctcagt gatgcgcacg 23358 ggtgggtagc tgaaacccac ggccaccagc tctgcctgtt ctctttcttc ttcgctgtcc 23418 tggctgatgt cttgcagagg gacatgtttg gtcttcctgg gcttcttctt gggagggatc 23478 gggggagggc tgttgctccg ctccggagac agggaggacc gcgaagtttc gctcaccagt 23538 accacctggc tctcggtaga agaacctgac cccacacggc ggtaggtgtt cctcttcggg 23598 ggcagaggtg gaggcgactg cgatggactg cggtccggcc tgggaggcgg atggctggca 23658 gagcctcttc cgcgttcggg ggtgtgctcc cggtggcggt cgcttgactg atttcctccg 23718 cggctggcca ttgtgttctc ctaggcagag aaacaacaga c atg gag act cag 23771 Met Glu Thr Gln cca tcg ctg cca aca ccg ctg caa gcg cca tca cac ctc gcc ccc 23816 Pro Ser Leu Pro Thr Pro Leu Gln Ala Pro Ser His Leu Ala Pro 4220 4225 4230 agc agc gac gag gag gaa cag agc tta acc acc cca cca ccc agt 23861 Ser Ser Asp Glu Glu Glu Gln Ser Leu Thr Thr Pro Pro Pro Ser 4235 4240 4245 ccc gcc acc acc acc tct acc cta gag gat gag gag gag gtc gac 23906 Pro Ala Thr Thr Thr Ser Thr Leu Glu Asp Glu Glu Glu Val Asp 4250 4255 4260 gca ccc cag gag atg cag gat atg gag gat gag aaa gcg gaa gag 23951 Ala Pro Gln Glu Met Gln Asp Met Glu Asp Glu Lys Ala Glu Glu 4265 4270 4275 att gag gca gat gtc gag cag gac ccg ggc tat gtg aca ccg gcg 23996 Ile Glu Ala Asp Val Glu Gln Asp Pro Gly Tyr Val Thr Pro Ala 4280 4285 4290 gag cac gag gag gag ctg aga cgc ttt cta gac aga gag gat aac 24041 Glu His Glu Glu Glu Leu Arg Arg Phe Leu Asp Arg Glu Asp Asn 4295 4300 4305 aac cgc cca gag cag caa gca gat ggc gac cac cag gag gct ggg 24086 Asn Arg Pro Glu Gln Gln Ala Asp Gly Asp His Gln Glu Ala Gly 4310 4315 4320 ctc ggg gat cat gtc gcc gac tac ctc acc ggg ctt ggc ggg gag 24131 Leu Gly Asp His Val Ala Asp Tyr Leu Thr Gly Leu Gly Gly Glu 4325 4330 4335 gac gtg ctc ctc aaa cat cta gca agg cag tcg atc ata gtt aaa 24176 Asp Val Leu Leu Lys His Leu Ala Arg Gln Ser Ile Ile Val Lys 4340 4345 4350 gac gca ctg ctc ggc cgc acc gaa gtg ccc atc agt gtg gaa gag 24221 Asp Ala Leu Leu Gly Arg Thr Glu Val Pro Ile Ser Val Glu Glu 4355 4360 4365 ctc agc cgc gcc tac gag ctc aac ctg ttc tca cct cgg gtg ccc 24266 Leu Ser Arg Ala Tyr Glu Leu Asn Leu Phe Ser Pro Arg Val Pro 4370 4375 4380 cct aag cgt cag cca aac ggc acc tgc gaa ccc aac cct cgc ctc 24311 Pro Lys Arg Gln Pro Asn Gly Thr Cys Glu Pro Asn Pro Arg Leu 4385 4390 4395 aac ttc tat ccg gcc ttt gct gtc cca gaa gtg ctg gct acc tac 24356 Asn Phe Tyr Pro Ala Phe Ala Val Pro Glu Val Leu Ala Thr Tyr 4400 4405 4410 cac att ttt ttc aag aac caa aag att cca gtt tcc tgc cgt gcc 24401 His Ile Phe Phe Lys Asn Gln Lys Ile Pro Val Ser Cys Arg Ala 4415 4420 4425 aac cgc acc cgc gcc gat gcc ctg ctc aac ttg ggt ccg ggc gct 24446 Asn Arg Thr Arg Ala Asp Ala Leu Leu Asn Leu Gly Pro Gly Ala 4430 4435 4440 cgc tta cct gat ata gct tcc ttg gaa gag gtt cca aag atc ttc 24491 Arg Leu Pro Asp Ile Ala Ser Leu Glu Glu Val Pro Lys Ile Phe 4445 4450 4455 gag ggt ctg ggc agt gat gag act cgg gct gca aat gct ctg caa 24536 Glu Gly Leu Gly Ser Asp Glu Thr Arg Ala Ala Asn Ala Leu Gln 4460 4465 4470 cag gga gag aat ggc atg gat gaa cat cac agc gcg ctg gta gag 24581 Gln Gly Glu Asn Gly Met Asp Glu His His Ser Ala Leu Val Glu 4475 4480 4485 ttg gag ggc gac aat gcc cgg ctg gca gtg ctc aag cgc agt atc 24626 Leu Glu Gly Asp Asn Ala Arg Leu Ala Val Leu Lys Arg Ser Ile 4490 4495 4500 gag gtc acc cat ttt gcc tac ccc gct gtc aac ctg ccc ccc aaa 24671 Glu Val Thr His Phe Ala Tyr Pro Ala Val Asn Leu Pro Pro Lys 4505 4510 4515 gtc atg agc gct gtc atg gat cag ctg ctc atc aag cga gca agc 24716 Val Met Ser Ala Val Met Asp Gln Leu Leu Ile Lys Arg Ala Ser 4520 4525 4530 ccc ctt tcc gaa gac cag aac atg cag gat cca gac gcc tct gac 24761 Pro Leu Ser Glu Asp Gln Asn Met Gln Asp Pro Asp Ala Ser Asp 4535 4540 4545 gag ggc aag ccg gtg gtc agt gac gag cag ctg tct cgc tgg ctg 24806 Glu Gly Lys Pro Val Val Ser Asp Glu Gln Leu Ser Arg Trp Leu 4550 4555 4560 ggc acc aac tcc ccg cga gac ttg gaa gag agg cgc aag ctt atg 24851 Gly Thr Asn Ser Pro Arg Asp Leu Glu Glu Arg Arg Lys Leu Met 4565 4570 4575 atg gct gta gtg cta gtc act gtg gag ctg gag tgt ctc cgc cgc 24896 Met Ala Val Val Leu Val Thr Val Glu Leu Glu Cys Leu Arg Arg 4580 4585 4590 ttt ttc acc gac cct gag acc ctg cgc aag ctc gag gag aac ttg 24941 Phe Phe Thr Asp Pro Glu Thr Leu Arg Lys Leu Glu Glu Asn Leu 4595 4600 4605 cac tat act ttc aga cat ggc ttc gtg cgc cag gca tgc aag atc 24986 His Tyr Thr Phe Arg His Gly Phe Val Arg Gln Ala Cys Lys Ile 4610 4615 4620 tcc aac gtg gag ctc acc aac ctg gtc tcc tac atg ggc att ttg 25031 Ser Asn Val Glu Leu Thr Asn Leu Val Ser Tyr Met Gly Ile Leu 4625 4630 4635 cat gag aac cgc ctg ggg caa agt gtg ctg cac acc acc ctg aag 25076 His Glu Asn Arg Leu Gly Gln Ser Val Leu His Thr Thr Leu Lys 4640 4645 4650 ggg gag gcc cgc cgc gac tac atc cgc gac tgt gtc tac ctc tac 25121 Gly Glu Ala Arg Arg Asp Tyr Ile Arg Asp Cys Val Tyr Leu Tyr 4655 4660 4665 ctc tgc cac acc tgg cag act ggc atg ggt gta tgg cag cag tgt 25166 Leu Cys His Thr Trp Gln Thr Gly Met Gly Val Trp Gln Gln Cys 4670 4675 4680 ttg gaa gag cag aac ctg aaa gag ctg gac aag ctc ttg cag aga 25211 Leu Glu Glu Gln Asn Leu Lys Glu Leu Asp Lys Leu Leu Gln Arg 4685 4690 4695 tcc ctc aaa gcc ctg tgg aca ggt ttt gac gag cgc acc gtc gcc 25256 Ser Leu Lys Ala Leu Trp Thr Gly Phe Asp Glu Arg Thr Val Ala 4700 4705 4710 tca gac ctg gca gac atc atc ttt ccc gag cgt ctc agg gtt act 25301 Ser Asp Leu Ala Asp Ile Ile Phe Pro Glu Arg Leu Arg Val Thr 4715 4720 4725 ctg cgc aac ggc ctg cct gac ttc atg agc cag agc atg ctt aac 25346 Leu Arg Asn Gly Leu Pro Asp Phe Met Ser Gln Ser Met Leu Asn 4730 4735 4740 aac ttt cgc tct ttc atc ctg gaa cgc tcc ggt atc ctg ccc gcc 25391 Asn Phe Arg Ser Phe Ile Leu Glu Arg Ser Gly Ile Leu Pro Ala 4745 4750 4755 acc tgc tgc gcg ctg ccc tcc gac ttt gtg cct ctc acc tac cgc 25436 Thr Cys Cys Ala Leu Pro Ser Asp Phe Val Pro Leu Thr Tyr Arg 4760 4765 4770 gag tgc ccc ccg ccg cta tgg agc cac tgc tac ctg ttc cgc ctg 25481 Glu Cys Pro Pro Pro Leu Trp Ser His Cys Tyr Leu Phe Arg Leu 4775 4780 4785 gcc aac tac ctc tcc tac cac tcg gat gtg atc gag gat gtg agc 25526 Ala Asn Tyr Leu Ser Tyr His Ser Asp Val Ile Glu Asp Val Ser 4790 4795 4800 gga gac ggc ctg ctg gag tgc cac tgc cgc tgc aat ctc tgc aca 25571 Gly Asp Gly Leu Leu Glu Cys His Cys Arg Cys Asn Leu Cys Thr 4805 4810 4815 ccc cac cgt tcc ctc gcc tgc aac ccc cag ttg ctg agc gag acc 25616 Pro His Arg Ser Leu Ala Cys Asn Pro Gln Leu Leu Ser Glu Thr 4820 4825 4830 cag atc atc ggc acc ttc gag ttg cag ggt ccc agc agc gaa ggc 25661 Gln Ile Ile Gly Thr Phe Glu Leu Gln Gly Pro Ser Ser Glu Gly 4835 4840 4845 gag ggg tct tct ccg ggg cag agt ctg aaa ctg acc ccg ggg cta 25706 Glu Gly Ser Ser Pro Gly Gln Ser Leu Lys Leu Thr Pro Gly Leu 4850 4855 4860 tgg acc tcc gcc tac ctg cgc aag ttc gcc ccc gaa gac tac cac 25751 Trp Thr Ser Ala Tyr Leu Arg Lys Phe Ala Pro Glu Asp Tyr His 4865 4870 4875 ccc tat gag att agg ttc tat gag gac caa tca cag ccg ccc aaa 25796 Pro Tyr Glu Ile Arg Phe Tyr Glu Asp Gln Ser Gln Pro Pro Lys 4880 4885 4890 gcc gag ctc tca gcc tgc gtc atc act cag ggg gca att ctc gcc 25841 Ala Glu Leu Ser Ala Cys Val Ile Thr Gln Gly Ala Ile Leu Ala 4895 4900 4905 caa ttg caa gcc atc caa aaa tcc cgc caa gaa ttt ctg ctg aaa 25886 Gln Leu Gln Ala Ile Gln Lys Ser Arg Gln Glu Phe Leu Leu Lys 4910 4915 4920 agg ggg aac ggg gtc tac ctc gac ccc cag acc ggt gag gag ctc 25931 Arg Gly Asn Gly Val Tyr Leu Asp Pro Gln Thr Gly Glu Glu Leu 4925 4930 4935 aac aca agg ttc cct cag gat gtc cca gcg ccg agg aag caa gaa 25976 Asn Thr Arg Phe Pro Gln Asp Val Pro Ala Pro Arg Lys Gln Glu 4940 4945 4950 gtt gaa agt gca gct gcc gcc ccc aga gga cat gga gga aga ctg 26021 Val Glu Ser Ala Ala Ala Ala Pro Arg Gly His Gly Gly Arg Leu 4955 4960 4965 gga cag tca ggc aga gga gga gga gat gga aga ttg gga cag cca 26066 Gly Gln Ser Gly Arg Gly Gly Gly Asp Gly Arg Leu Gly Gln Pro 4970 4975 4980 ggc aga gga ggt gga cag cct gga gga aga cag ttt gga gga gga 26111 Gly Arg Gly Gly Gly Gln Pro Gly Gly Arg Gln Phe Gly Gly Gly 4985 4990 4995 aga cga gga ggc aga gga ggt gga aga agc agc cgc cgc caa aca 26156 Arg Arg Gly Gly Arg Gly Gly Gly Arg Ser Ser Arg Arg Gln Thr 5000 5005 5010 gtt gtc ctc ggc agc gga gac aag caa ggt ccc aga cag cag cag 26201 Val Val Leu Gly Ser Gly Asp Lys Gln Gly Pro Arg Gln Gln Gln 5015 5020 5025 cag cac ggc tac aat ctc cgc tcc ggg tcg ggg ggc cca gcg gcg 26246 Gln His Gly Tyr Asn Leu Arg Ser Gly Ser Gly Gly Pro Ala Ala 5030 5035 5040 tcc caa cag tagatgggac gagaccgggc gattcccgaa cccgaccacc 26295 Ser Gln Gln 5045 gcttccaaga ccggtaagaa ggagcggcag ggatacaagt cctggcgggg gcataagaat 26355 gccatcatct cctgcttgca tgaatgcggg ggcaacatat ccttcacccg gcgctacctg 26415 ctcttccacc acggggtgaa cttcccccgc aatgtcttgc attactaccg tcacctccac 26475 agcccctact acagccagca agtcccggca gcctcggcag agaaagacag cagcagcagc 26535 ggggacctcc agcagaaaac cagcagcagc agttagaaaa tccagtgcag caggaggagg 26595 actgaggatc acagcgaacg agccagcgca gacccgagag ctgagaaaca ggatctttcc 26655 aaccctctat gccatcttcc agcagagtcg ggggcaagag caggaactga aagtaaaaaa 26715 ccgatctctg cgctcgctca cccgaagttg tttgtatcac aagagcgaag accaacttca 26775 gcgcactctc gaggacgccg aggctctctt caacaagtac tgcgcgctga ctcttaaaga 26835 gtagcccgcg cccgcgctcg ctcgaaaaag gcgggaatta cgtcaccctt ggcacctgtc 26895 ctttgccctc gtc atg agt aaa gaa att ccc acg cct tac atg tgg agc 26944 Met Ser Lys Glu Ile Pro Thr Pro Tyr Met Trp Ser 5050 5055 tat cag ccc caa atg gga ctg gca gcc ggc gcc tcc cag gac tac 26989 Tyr Gln Pro Gln Met Gly Leu Ala Ala Gly Ala Ser Gln Asp Tyr 5060 5065 5070 tcc acc cgc atg aat tgg ctc agc gcc ggc ccc tcg atg atc tca 27034 Ser Thr Arg Met Asn Trp Leu Ser Ala Gly Pro Ser Met Ile Ser 5075 5080 5085 cgg gtt aat gat ata cga gct tac cga aac cag tta ctc cta gaa 27079 Arg Val Asn Asp Ile Arg Ala Tyr Arg Asn Gln Leu Leu Leu Glu 5090 5095 5100 cag tca gct ctc acc acc aca ccc cgc caa cac ctt aat ccc cgg 27124 Gln Ser Ala Leu Thr Thr Thr Pro Arg Gln His Leu Asn Pro Arg 5105 5110 5115 aat tgg ccc gcc gcc ctg gtg tac cag gaa act ccc gct ccc acc 27169 Asn Trp Pro Ala Ala Leu Val Tyr Gln Glu Thr Pro Ala Pro Thr 5120 5125 5130 acc gta cta ctt cct cga gac gcc cag gcc gaa gtt cag atg act 27214 Thr Val Leu Leu Pro Arg Asp Ala Gln Ala Glu Val Gln Met Thr 5135 5140 5145 aac gca ggt gta cag ctg gcg ggc ggt tcc gcc ctg tgt cgt cac 27259 Asn Ala Gly Val Gln Leu Ala Gly Gly Ser Ala Leu Cys Arg His 5150 5155 5160 cgg cct cag cag agt ata aaa cgc ctg gtg atc aga ggc cga ggt 27304 Arg Pro Gln Gln Ser Ile Lys Arg Leu Val Ile Arg Gly Arg Gly 5165 5170 5175 atc cag ctc aac gac gag tcg gtg agc tct tcg ctt ggt ctg cga 27349 Ile Gln Leu Asn Asp Glu Ser Val Ser Ser Ser Leu Gly Leu Arg 5180 5185 5190 cca gac gga gtc ttc cag atc gcc ggc tgt ggg aga tct tcc ttc 27394 Pro Asp Gly Val Phe Gln Ile Ala Gly Cys Gly Arg Ser Ser Phe 5195 5200 5205 act cct cgt cag gct gtc ctg act ttg gag agt tcg tcc tcg cag 27439 Thr Pro Arg Gln Ala Val Leu Thr Leu Glu Ser Ser Ser Ser Gln 5210 5215 5220 ccc cgc tcg ggc ggc atc ggg act ctc cag ttt gtg gag gag ttt 27484 Pro Arg Ser Gly Gly Ile Gly Thr Leu Gln Phe Val Glu Glu Phe 5225 5230 5235 act ccc tct gtc tac ttc aac ccc ttc tcc ggc tct cct ggc cag 27529 Thr Pro Ser Val Tyr Phe Asn Pro Phe Ser Gly Ser Pro Gly Gln 5240 5245 5250 tac ccg gac gag ttc ata ccg aac ttc gac gca atc agc gag tca 27574 Tyr Pro Asp Glu Phe Ile Pro Asn Phe Asp Ala Ile Ser Glu Ser 5255 5260 5265 gtg gat ggc tat gat tgatgtctgg tggcgcggct gagttagctc gactgcgaca 27629 Val Asp Gly Tyr Asp 5270 tctagaccac tgccgccgct ttcgctgttt cgcccgggaa ctcaccgagt tcatctactt 27689 cgaactcccc gaggagcacc ctcagggacc ggcccacgga gtgcggatta ccatcgaagg 27749 ggggatagac tctcacctgc atcggatctt ctgccagcga cccgtgctga tcgagcgcga 27809 ccagggaact acaacagtct ccatctactg catctgtaac caccccggat tgc atg 27865 Met 5275 aaa gcc ttt gct gtc tta ttt gtg ctg agt tta ata aaa act gag 27910 Lys Ala Phe Ala Val Leu Phe Val Leu Ser Leu Ile Lys Thr Glu 5280 5285 5290 tta aga ctc acc ttc gga cta ccg ctt ctt caa ccc gga ctt tac 27955 Leu Arg Leu Thr Phe Gly Leu Pro Leu Leu Gln Pro Gly Leu Tyr 5295 5300 5305 aac acc agc cag acc ctc cgt tcc agc cag aag aac cag acc ctt 28000 Asn Thr Ser Gln Thr Leu Arg Ser Ser Gln Lys Asn Gln Thr Leu 5310 5315 5320 cct ctg atc cag gac tct aat tct acc tcc cca gcg cct ttc cct 28045 Pro Leu Ile Gln Asp Ser Asn Ser Thr Ser Pro Ala Pro Phe Pro 5325 5330 5335 act aac ctt ccc gtt act aac aac ctc gga gct cag ctg cat cac 28090 Thr Asn Leu Pro Val Thr Asn Asn Leu Gly Ala Gln Leu His His 5340 5345 5350 cgc ttc tcc aga agc ctc ctt tct gcc aat att act act ccc aga 28135 Arg Phe Ser Arg Ser Leu Leu Ser Ala Asn Ile Thr Thr Pro Arg 5355 5360 5365 acc gga ggt gag ctc cgt ggt ctc cct act gac aac ccc tgg gtg 28180 Thr Gly Gly Glu Leu Arg Gly Leu Pro Thr Asp Asn Pro Trp Val 5370 5375 5380 gta gcg ggt ttt gta gcg cta gga gta gtt gcg ggt ggg ttg gtg 28225 Val Ala Gly Phe Val Ala Leu Gly Val Val Ala Gly Gly Leu Val 5385 5390 5395 ctt ata ctc tgc tac cta tac aca cct tgc tgt gct tat tta gta 28270 Leu Ile Leu Cys Tyr Leu Tyr Thr Pro Cys Cys Ala Tyr Leu Val 5400 5405 5410 gtg ttg tgt tgc tgg ttt aag aaa tgg ggg tcg tac tagtatcgct 28316 Val Leu Cys Cys Trp Phe Lys Lys Trp Gly Ser Tyr 5415 5420 tgctttactt tcgcttttgg gtctgggctc tgctacgcta agaaatcagc ctttgctatt 28376 agatcccgat gatgttgatc catgtctgga ctttgatcca gagaactgca cactcacttt 28436 tgcacctgaa acaagtcgct tctgtggagt tgttattagg tgcggatttg aatgcaggtc 28496 cattgagatt acacacaata acaaaacttg gaacaatacc ttattcacaa tatggcaacc 28556 aggagatcct cagtggtata ctgtctctgt ccggggtcct gacggttcca tccgcatggc 28616 taataacact ttcatttttg ctgaaatgtg cgatatggcc atgttcatga gcagacagta 28676 tgacct atg gcc tcc cag caa aga gaa cat tgt ggc ttt ctc cat tgc 28724 Met Ala Ser Gln Gln Arg Glu His Cys Gly Phe Leu His Cys 5425 5430 5435 tta ttg ctt gtg tac ttg cat cat cac tgc tat cat gtg tgt gag 28769 Leu Leu Leu Val Tyr Leu His His His Cys Tyr His Val Cys Glu 5440 5445 5450 cat aca ctt gct tat agc cat tcg ccc aaa aaa caa tca aga aaa 28814 His Thr Leu Ala Tyr Ser His Ser Pro Lys Lys Gln Ser Arg Lys 5455 5460 5465 aga gaa aat gcc ctg att ata aat ttc tat tta cag aaa atg acc 28859 Arg Glu Asn Ala Leu Ile Ile Asn Phe Tyr Leu Gln Lys Met Thr 5470 5475 5480 tct gtt tca gct ctc ata ttt gct act att atg gct gtt caa gga 28904 Ser Val Ser Ala Leu Ile Phe Ala Thr Ile Met Ala Val Gln Gly 5485 5490 5495 cag gct gct caa gga cag aca ctt att aat gtt cat cct gga act 28949 Gln Ala Ala Gln Gly Gln Thr Leu Ile Asn Val His Pro Gly Thr 5500 5505 5510 aat cat acc ttg gtg gtt cct aat aac tat tca aat att gaa tgg 28994 Asn His Thr Leu Val Val Pro Asn Asn Tyr Ser Asn Ile Glu Trp 5515 5520 5525 caa tgg ttc aca aac aac gta tgg tat gaa cca tgc gaa cat tac 29039 Gln Trp Phe Thr Asn Asn Val Trp Tyr Glu Pro Cys Glu His Tyr 5530 5535 5540 agc cta ttc att tgc aat cat aat tta act tta atc aat gtc agc 29084 Ser Leu Phe Ile Cys Asn His Asn Leu Thr Leu Ile Asn Val Ser 5545 5550 5555 aca ata cac aaa gga tac tat tat aga tat gac aac cac agc att 29129 Thr Ile His Lys Gly Tyr Tyr Tyr Arg Tyr Asp Asn His Ser Ile 5560 5565 5570 gat cct aca ata tat cta gta cgt gta aat cca att aac aaa cct 29174 Asp Pro Thr Ile Tyr Leu Val Arg Val Asn Pro Ile Asn Lys Pro 5575 5580 5585 ata ccc aaa gct ttc tct aga act aca ata caa aac ttt aaa aca 29219 Ile Pro Lys Ala Phe Ser Arg Thr Thr Ile Gln Asn Phe Lys Thr 5590 5595 5600 gca att tta ctt aat ttt aaa acc aaa aat att aca ggc aat ata 29264 Ala Ile Leu Leu Asn Phe Lys Thr Lys Asn Ile Thr Gly Asn Ile 5605 5610 5615 ctt ccc act act ccc act gaa aaa aat aca cct aat tca ata ttt 29309 Leu Pro Thr Thr Pro Thr Glu Lys Asn Thr Pro Asn Ser Ile Phe 5620 5625 5630 gaa atc atc att gca ctg tta gca gta ggc ata aca atc ata cta 29354 Glu Ile Ile Ile Ala Leu Leu Ala Val Gly Ile Thr Ile Ile Leu 5635 5640 5645 tgt atg ata att tat gct cac tgt tat aaa aaa att cac cac aaa 29399 Cys Met Ile Ile Tyr Ala His Cys Tyr Lys Lys Ile His His Lys 5650 5655 5660 aaa gaa cca cta cta agc ttt taatttcttt tttatacagc c atg att ttc 29450 Lys Glu Pro Leu Leu Ser Phe Met Ile Phe 5665 5670 ttc gca act ctt att act att ggc att gtt caa ggg caa gat atc 29495 Phe Ala Thr Leu Ile Thr Ile Gly Ile Val Gln Gly Gln Asp Ile 5675 5680 5685 aca att gga tat gta ggc aat aat att acc cta tta ggt ccc cca 29540 Thr Ile Gly Tyr Val Gly Asn Asn Ile Thr Leu Leu Gly Pro Pro 5690 5695 5700 aca gga aca atc cct acc tgg tac aaa ata tat gaa aga ggg tgg 29585 Thr Gly Thr Ile Pro Thr Trp Tyr Lys Ile Tyr Glu Arg Gly Trp 5705 5710 5715 tgg att aga ccc tgc gac caa gga ggt agt aaa tac att tgt ggt 29630 Trp Ile Arg Pro Cys Asp Gln Gly Gly Ser Lys Tyr Ile Cys Gly 5720 5725 5730 aga gac ata acc atc acc aat ctt aat aaa aac gat aat ggc tac 29675 Arg Asp Ile Thr Ile Thr Asn Leu Asn Lys Asn Asp Asn Gly Tyr 5735 5740 5745 tat ttt tgc aat aac tat gga ggt ggt aaa aag tct tac aca ctt 29720 Tyr Phe Cys Asn Asn Tyr Gly Gly Gly Lys Lys Ser Tyr Thr Leu 5750 5755 5760 gaa gta aga gac ccc acc act tta gca cca cat acc act ttc tcc 29765 Glu Val Arg Asp Pro Thr Thr Leu Ala Pro His Thr Thr Phe Ser 5765 5770 5775 agc agc acg tct aga aac aca cat gag gca gct tat gcc aga gca 29810 Ser Ser Thr Ser Arg Asn Thr His Glu Ala Ala Tyr Ala Arg Ala 5780 5785 5790 atg ctt caa aaa att aat gaa aca ata aat tct aca atc tct cat 29855 Met Leu Gln Lys Ile Asn Glu Thr Ile Asn Ser Thr Ile Ser His 5795 5800 5805 aat cca gac gaa att ccc aaa tca atg att ggc att att gta gcc 29900 Asn Pro Asp Glu Ile Pro Lys Ser Met Ile Gly Ile Ile Val Ala 5810 5815 5820 gtg gca gtt gga atg gca atc ata ata att tgt atg atc gtc tat 29945 Val Ala Val Gly Met Ala Ile Ile Ile Ile Cys Met Ile Val Tyr 5825 5830 5835 gct tgc tgc tat aga aag ttt caa gat gaa aaa gga gac cca cta 29990 Ala Cys Cys Tyr Arg Lys Phe Gln Asp Glu Lys Gly Asp Pro Leu 5840 5845 5850 cta agc ttt gat att taatttcttt atagaaac atg aaa gga gta ggt atc 30041 Leu Ser Phe Asp Ile Met Lys Gly Val Gly Ile 5855 5860 cta gtt ctt tca act tta atc tac tca gtg atc cct atc agc atc 30086 Leu Val Leu Ser Thr Leu Ile Tyr Ser Val Ile Pro Ile Ser Ile 5865 5870 5875 aat gtg cag act act tta aat gaa act gga aac cac tca act acc 30131 Asn Val Gln Thr Thr Leu Asn Glu Thr Gly Asn His Ser Thr Thr 5880 5885 5890 tca cat aca cct ccc ccg ctt tct acc cac cct caa tcc aaa gat 30176 Ser His Thr Pro Pro Pro Leu Ser Thr His Pro Gln Ser Lys Asp 5895 5900 5905 gcc ata caa cta caa ctc acc atc ctt att gtg att ggg tta act 30221 Ala Ile Gln Leu Gln Leu Thr Ile Leu Ile Val Ile Gly Leu Thr 5910 5915 5920 atc ctt gct gtt atc ctt tac ttt atc ttt tgc cgc caa ata ccc 30266 Ile Leu Ala Val Ile Leu Tyr Phe Ile Phe Cys Arg Gln Ile Pro 5925 5930 5935 aat gta gtt aag aaa cct acc aga cgt ccc atc tat cga tca ata 30311 Asn Val Val Lys Lys Pro Thr Arg Arg Pro Ile Tyr Arg Ser Ile 5940 5945 5950 atc agc aaa ccc cac atg gct cta aat gaa att taatctttct 30354 Ile Ser Lys Pro His Met Ala Leu Asn Glu Ile 5955 5960 cttcacagta tggtgatcaa ct atg atc cct aga aat ttc ttc ttc acc 30403 Met Ile Pro Arg Asn Phe Phe Phe Thr 5965 5970 ata ctt atc tgc gct ttc aat gtc tgt gct aca ttc gcc aca gtc 30448 Ile Leu Ile Cys Ala Phe Asn Val Cys Ala Thr Phe Ala Thr Val 5975 5980 5985 gcc aat gtg aca cca gat tgt ata ggg gca ttt gct tcc tac gta 30493 Ala Asn Val Thr Pro Asp Cys Ile Gly Ala Phe Ala Ser Tyr Val 5990 5995 6000 cta ttt gcc ttc att acc tgc atc tgc gtt tgt agc ata gtc tgc 30538 Leu Phe Ala Phe Ile Thr Cys Ile Cys Val Cys Ser Ile Val Cys 6005 6010 6015 ctg gtt atc aac ttc ttt caa cta gta gac tgg gtt ttt gta cgc 30583 Leu Val Ile Asn Phe Phe Gln Leu Val Asp Trp Val Phe Val Arg 6020 6025 6030 att gcc tac cta cga cat cac cct gaa tac cgc aac caa aat gtt 30628 Ile Ala Tyr Leu Arg His His Pro Glu Tyr Arg Asn Gln Asn Val 6035 6040 6045 gca gca att ctt agg ctc att taaaaccatg caaactctgc tactgcttct 30679 Ala Ala Ile Leu Arg Leu Ile 6050 gctagttata caccaatgtg cctcaaaccc cacaagcccc acaagattag atctaagaaa 30739 atgtaaattt caagaaccat ggaaattcct tgattgctat catgaaacat ctgatttccc 30799 cacatactgg attacaatca ttggggttgt taatctagtc tcttgcacac tattctcttt 30859 ccttgtttac cacttatttg attttggatg gaacgccctt aatgcactca cttacccaca 30919 agaaccagag gaacatatac cactacagaa catacaacca ttagcactag tagaatatga 30979 aaatgagcca cagcctccac tactccctgc cattagctac ttcaacctaa ccggtggag 31038 atg act gac cca cac gcc gct gct gag gaa cta ctt gat atg gac 31083 Met Thr Asp Pro His Ala Ala Ala Glu Glu Leu Leu Asp Met Asp 6055 6060 6065 ggc cgt gcc tcc gaa cag cgc ctc gct caa cta cgc att cgc cag 31128 Gly Arg Ala Ser Glu Gln Arg Leu Ala Gln Leu Arg Ile Arg Gln 6070 6075 6080 cag cag gaa cgt gcc gcc aag gag ctc agg gat gcc att gag att 31173 Gln Gln Glu Arg Ala Ala Lys Glu Leu Arg Asp Ala Ile Glu Ile 6085 6090 6095 cac cag tgc aaa aaa ggc ata ttc tgc ttg gta aaa caa gcc aag 31218 His Gln Cys Lys Lys Gly Ile Phe Cys Leu Val Lys Gln Ala Lys 6100 6105 6110 atc tcc tac gag atc acc gct aac gac cac cgc ctc tca tat gag 31263 Ile Ser Tyr Glu Ile Thr Ala Asn Asp His Arg Leu Ser Tyr Glu 6115 6120 6125 ctt ggc ccg cag cgt cag aaa ttc act tgc atg gtg gga atc aac 31308 Leu Gly Pro Gln Arg Gln Lys Phe Thr Cys Met Val Gly Ile Asn 6130 6135 6140 ccc ata gtc atc acc cag caa gct gga gat acc aag ggt tgc atc 31353 Pro Ile Val Ile Thr Gln Gln Ala Gly Asp Thr Lys Gly Cys Ile 6145 6150 6155 cat tgt tcc tgt gaa tcc acc gag tgc atc tac acc ctg ctg aag 31398 His Cys Ser Cys Glu Ser Thr Glu Cys Ile Tyr Thr Leu Leu Lys 6160 6165 6170 acc ctc tgc ggc ctt cga gac ctc cta ccc atg aac taatcaacaa 31444 Thr Leu Cys Gly Leu Arg Asp Leu Leu Pro Met Asn 6175 6180 6185 cccctaccct cttcccatta aaatccaatt aataaaattc acttacttaa aatcagaaac 31504 aaagtttttg tccaagttgt tttcaaccag cacctcactt ccctcttccc aactctggta 31564 ctctaagcct cggcgggtgg catacttcct ccacactttg aaagggatgt caaattttag 31624 ttcttctttt cccacaatct tcatttcttt attcccag atg gcc aaa cga gct 31677 Met Ala Lys Arg Ala 6190 cgt cta agc agc tcc ttc aac ccg gtc tac ccc tat gaa gat gaa 31722 Arg Leu Ser Ser Ser Phe Asn Pro Val Tyr Pro Tyr Glu Asp Glu 6195 6200 6205 agc agt tca caa cac cca ttt ata aac cct ggt ttc att tcc cct 31767 Ser Ser Ser Gln His Pro Phe Ile Asn Pro Gly Phe Ile Ser Pro 6210 6215 6220 aat ggg ttt aca caa agt cca gac gga gct ctt aca ctc aag tgt 31812 Asn Gly Phe Thr Gln Ser Pro Asp Gly Ala Leu Thr Leu Lys Cys 6225 6230 6235 gtt gcc cct ctt act acc acc agt ggc tcc ctg gat att aaa gta 31857 Val Ala Pro Leu Thr Thr Thr Ser Gly Ser Leu Asp Ile Lys Val 6240 6245 6250 gga ggg ggg ctt aag gta gac tcc act gat ggg tcc tta gaa gaa 31902 Gly Gly Gly Leu Lys Val Asp Ser Thr Asp Gly Ser Leu Glu Glu 6255 6260 6265 aac ata agc act aca gca cca ctt aac aaa tct aat cat tcc ata 31947 Asn Ile Ser Thr Thr Ala Pro Leu Asn Lys Ser Asn His Ser Ile 6270 6275 6280 gga tta gca gtg gga aat gga tta caa aca aat gaa agc aaa cta 31992 Gly Leu Ala Val Gly Asn Gly Leu Gln Thr Asn Glu Ser Lys Leu 6285 6290 6295 tgt gcc aaa tta gga gag gaa ctt acc ttt gat tct tcc aat gcc 32037 Cys Ala Lys Leu Gly Glu Glu Leu Thr Phe Asp Ser Ser Asn Ala 6300 6305 6310 att aca ata aaa aat aac act tta tgg aca gga gca aaa cca agt 32082 Ile Thr Ile Lys Asn Asn Thr Leu Trp Thr Gly Ala Lys Pro Ser 6315 6320 6325 act aac tgt aaa att caa gaa gat gca gat gcc cta gac tgc aag 32127 Thr Asn Cys Lys Ile Gln Glu Asp Ala Asp Ala Leu Asp Cys Lys 6330 6335 6340 cta act cta gtc ctt gta aaa aat gga gga cta gta aat gca tat 32172 Leu Thr Leu Val Leu Val Lys Asn Gly Gly Leu Val Asn Ala Tyr 6345 6350 6355 gtg tca tta ata gga gac tca gac tat gtt aat aca cta ttc act 32217 Val Ser Leu Ile Gly Asp Ser Asp Tyr Val Asn Thr Leu Phe Thr 6360 6365 6370 aaa aag act gca tca atc agc gta gaa ctt gcc ttt gat agc tcc 32262 Lys Lys Thr Ala Ser Ile Ser Val Glu Leu Ala Phe Asp Ser Ser 6375 6380 6385 ggt caa ata ctt act agc cta tct tct cta aaa act agt ctc aac 32307 Gly Gln Ile Leu Thr Ser Leu Ser Ser Leu Lys Thr Ser Leu Asn 6390 6395 6400 ttt aaa cac aac caa gac atg gcc act gaa act atc agt gcc aaa 32352 Phe Lys His Asn Gln Asp Met Ala Thr Glu Thr Ile Ser Ala Lys 6405 6410 6415 ggc ttc atg cct agt acc act gct tat ccc ttt aac acc cag gct 32397 Gly Phe Met Pro Ser Thr Thr Ala Tyr Pro Phe Asn Thr Gln Ala 6420 6425 6430 act tct tct aga gac aat gaa gat tac att ttt ggt aaa tgt tac 32442 Thr Ser Ser Arg Asp Asn Glu Asp Tyr Ile Phe Gly Lys Cys Tyr 6435 6440 6445 tac aga gcc tca tat gga gct cta tac act ttg gat gtt act gta 32487 Tyr Arg Ala Ser Tyr Gly Ala Leu Tyr Thr Leu Asp Val Thr Val 6450 6455 6460 ata ctc aac aga cgt atg acc gct gct gga atg gct tat gca atg 32532 Ile Leu Asn Arg Arg Met Thr Ala Ala Gly Met Ala Tyr Ala Met 6465 6470 6475 aac ttt acg tgg ctt ctt gac gcg aca gat gcc cca gaa aat acc 32577 Asn Phe Thr Trp Leu Leu Asp Ala Thr Asp Ala Pro Glu Asn Thr 6480 6485 6490 aca acc acc ctg gtc acc tcc ccc ttc tcc ttt tcc tat att aga 32622 Thr Thr Thr Leu Val Thr Ser Pro Phe Ser Phe Ser Tyr Ile Arg 6495 6500 6505 gaa gat gat gac tgacaaagaa taaagttcaa cttttttatt gaaaatcagt 32674 Glu Asp Asp Asp 6510 ttacaggata cgagtagtta ttttgcctcc cccttcccat ttcatagaat acaccaatct 32734 ctccccacgc acagctttaa acatttggat tccatttgag atagtcatgg atttagattc 32794 cacattccac acagtttcag agctagataa tcttggatca gtgatagata taaatccatc 32854 ggggcagtcc ttcaaggtga tttcacagtc cagttgctgt ggctgcggct ccggagtctg 32914 gatcagagtc atctggaaga agaacgatgg gagtcataat ccgagaacgg gatcgggcgg 32974 ttgtgtctca tcaaaccccg aagcagtcgc tgtctgcgcc gctccgtgcg actgctgcta 33034 atgggatcgg ggtccacagt ctctcgaagc atgattctaa tagccctcaa cattaacatc 33094 ctggtacgat gcgcacagca acgcatcctg atctcactta ggtcacagca gtaagtacaa 33154 cacatcacca caatgttgtt taacaggcca taattaaagg cgctccagcc aaaactcatt 33214 tcaggaataa tttgccccgc gtggccatcg taccaaatcc tgatgaaaat caaatggcgc 33274 cccctccaga atacactgcc cacatacatg atctccttag gcatatgcat attcacaatc 33334 tctcggtacc atggacagcg ctggttaatc atgcagcccc gaataacctt ccggaaccaa 33394 atggccagca atgcgccccc agcaatacat tgaagagaac cctgtcgatt acagtgacaa 33454 tggagaaccc acttctctcg cccatggatc acttgggaat aaaatatatc tattgtggca 33514 caacacagac ataaatgcat acatcttctc atcaccctta actcttcagg ggttaaaaac 33574 atatcccagg gaataggaag ctcttgcaaa acagtaaagg tggcagaaca aggcagaccg 33634 cgaacataac ttacactgtg catggtcaag gtattgcaat ctggtaacag cggatgctct 33694 tcagtcatag aagctctggt ttcactttcc tcacaacgtg gtaaaggggc cctcagttga 33754 ggttccctgg tgtaaggatg gtgtctggcg cacgatgtcg agcgtgcacg cgacctcgtt 33814 gtaatggagc tgcttcctga cattctcgta ttttgcatgg cagaacctag ccttggcaca 33874 acacacttct cttcgccttc tatcccgtcg cctagcacgt tcagtatggt aattgaagta 33934 cagccattcc cgtagattgg tcaaaagctc ctcggcttca gttgtcataa aaactccatc 33994 atatcttact gctctgataa aatcattcac tgtagaatgg gcaatgccca gccaggcaat 34054 gcaattagct tgtgtttcaa ccaaaggagg gggaggaaga catggaagaa ccataattaa 34114 tttttattcc agacgatccc gcagtatttc tacatggaga tcacgaagat ggcacctctc 34174 gcccccactg tgttgatgaa aaatgacagc taggtcaaac ataatgcgat tttccaggtg 34234 ctcaacggtg gcttcaagca aagcctccaa acgtacatcc aaaaacaaaa gaacagcaaa 34294 agcaggagca ttttctaatt cctcaatcat catattacat tcctgtacca ttcccaaata 34354 attttcatct ttccatcctt gaattattcg tgttatttca tctggtaaat ccaatccaca 34414 catgagaaat agctcccgaa gggcgccctc caccggcatt cttaagcaca ccctcatagt 34474 gaaaaaatat cgtgctcctc tgtcacctgc agcaaattaa gaatggcaac atcatactgg 34534 atgccactgg ctctaagttc ttctctaagt tccagttgta aaaactcttg catatcatcg 34594 ccaaactgct tggccatagg tcctccagga ataagagctg gggacgctac agtgcagaac 34654 aagcgcatgc caccccaatt gcctccagca aaagttaggt tgcaatatgc atactgagaa 34714 cctccagtga tatcatccag tgtactggaa agataatcag gcagagcttc tcgtatacaa 34774 ttaataatag aaaagtctgc cagatgaaca tttaaagcct gtgggatgca gatgcaataa 34834 gttatcgcgc tgcgctccaa cattgttagt atggttagtc tgtaaaaaca aaaaacaaaa 34894 attacatcac gctgtactgg cgaacgggtg gataaatcac tctctccaac accaggcagg 34954 ctacagggtc tccagcgcga ccctcgtaaa acctgtcagt atgattaaaa agcatcaccg 35014 aaagagattg ttgatggcca gcatatatta tttgcgatga agcatacaaa ccagaagtgt 35074 tagtatcagt taaagaaaaa aatcggccaa gatagcatct cggaacgatt atgctcaatc 35134 tcaaatgcag caaagcgaca cctcgcggat gcaaagtaaa atccacagga gcataaaaaa 35194 tgtaattatt cccctcttgc acaggcagcc tagctcccgg cccctccagg atcacataca 35254 aagcctcagc agccatagct taccgcgcaa atcaggcaca gcagtcagat aacgagaaag 35314 ctgtgaactg actgcccagc cgtgcgcaat atatagagaa cccttacact gacgtaattg 35374 gacaaagtct aaaaaatccc gccaaaaacc agcacacgcc cagaactgtg tcacccgcta 35434 aaaaataatt ttcacttcct cgttccgtga atgacgtcag ttcccctttc ccacgagccg 35494 tcacttccgg gcatcttgca acgtcacctc cccgcgccgg cccgcccctt ttgaccgttg 35554 aacccgctgg ccaatcccct tccgccctcc attttcaaaa gctcatttgc atgttggcac 35614 cgttccattt ataaggtata ttattgatga tg 35646 <210> 72 <211> 495 <212> PRT <213> Simian adenovirus 29 <400> 72 Met Asp Pro Thr Asn Pro Leu Gln Gln Gly Ile Arg Leu Gly Phe His 1 5 10 15 Ser Ser Ser Phe Val Glu Asn Met Glu Gly Pro Gln Ala Glu Asp Asn 20 25 30 Leu Arg Leu Leu Ala Ser Ala Ala Ser Gly Arg Ser Ser Asn Pro Glu 35 40 45 Thr Pro Thr Gly His Ala Ser Gly Phe Gly Gly Gly Ala Ala Gly Gly 50 55 60 Gln Pro Glu Ser Arg Leu Gly Pro Ser Gly Gly Gly Gly Gly Gly Val 65 70 75 80 Ala Asp Leu Phe Pro Glu Leu Arg Arg Val Leu Thr Arg Ser Thr Ser 85 90 95 Ser Gly Gln Asp Arg Gly Ile Lys Arg Glu Arg Asn Ala Ser Gly His 100 105 110 Asn Ser Arg Thr Glu Leu Ala Leu Ser Leu Met Ser Arg Ser Arg Pro 115 120 125 Glu Thr Ile Trp Trp His Glu Val Gln Ser Glu Gly Arg Asp Glu Val 130 135 140 Ser Ile Leu Gln Glu Lys Tyr Ser Leu Glu Gln Ile Lys Thr Cys Trp 145 150 155 160 Leu Glu Pro Glu Asp Asp Trp Glu Val Ala Ile Arg Asn Tyr Ala Lys 165 170 175 Ile Ser Leu Arg Pro Asp Lys Gln Tyr Lys Ile Thr Lys Lys Ile Asn 180 185 190 Ile Arg Asn Ala Cys Tyr Ile Ala Gly Asn Gly Ala Glu Val Ile Ile 195 200 205 Asp Thr Pro Asp Lys Thr Ala Phe Arg Cys Cys Met Met Gly Met Trp 210 215 220 Pro Gly Val Ala Gly Met Glu Ala Val Thr Leu Met Asn Ile Arg Phe 225 230 235 240 Arg Gly Asp Gly Tyr Asn Gly Ile Val Phe Met Ala Asn Thr Lys Leu 245 250 255 Ile Leu His Gly Cys Ser Phe Phe Gly Phe Asn Asn Thr Cys Val Glu 260 265 270 Ser Trp Gly Gln Val Ser Ile Arg Gly Cys Ser Phe Tyr Ala Cys Trp 275 280 285 Ile Ala Leu Ser Gly Arg Thr Lys Ser Gln Leu Ser Val Lys Lys Cys 290 295 300 Met Phe Glu Arg Cys Asn Leu Gly Ile Leu Asn Glu Gly Glu Ala Arg 305 310 315 320 Val Arg His Cys Ala Ala Thr Glu Thr Gly Cys Phe Ile Leu Ile Lys 325 330 335 Gly Asn Ala Ser Val Lys His Asn Met Ile Cys Gly Pro Ser Asp Glu 340 345 350 Arg Pro Tyr Gln Met Leu Thr Cys Ala Gly Gly His Cys Asn Met Leu 355 360 365 Ala Thr Val His Ile Val Ser His Ala Arg Lys Lys Trp Pro Val Phe 370 375 380 Glu His Asn Val Met Thr Lys Cys Thr Met His Ala Gly Gly Arg Arg 385 390 395 400 Gly Met Phe Met Pro Tyr Gln Cys Asn Met Asn His Val Lys Val Met 405 410 415 Leu Glu Pro Asp Ala Phe Ser Arg Met Ser Leu Thr Gly Ile Phe Asp 420 425 430 Met Asn Val Gln Leu Trp Lys Ile Leu Arg Tyr Asp Glu Thr Lys Ser 435 440 445 Arg Val Arg Ala Cys Glu Cys Gly Gly Lys His Ala Arg Phe Gln Pro 450 455 460 Val Cys Val Asp Val Thr Glu Asp Leu Arg Pro Asp His Leu Val Leu 465 470 475 480 Ala Cys Thr Gly Ala Glu Phe Gly Ser Ser Gly Glu Glu Thr Asp 485 490 495 <210> 73 <211> 138 <212> PRT <213> Simian adenovirus 29 <400> 73 Met Ser Gly Ser Ala Ser Phe Glu Gly Gly Val Phe Ser Pro Tyr Leu 1 5 10 15 Thr Gly Arg Leu Pro Pro Trp Ala Gly Val Arg Gln Asn Val Met Gly 20 25 30 Ser Thr Val Asp Gly Arg Pro Val Gln Pro Ala Asn Ser Ser Thr Leu 35 40 45 Thr Tyr Ala Thr Leu Ser Ser Ser Ser Leu Asp Ala Ala Ala Ala Ala 50 55 60 Ala Ala Ser Ala Ala Ala Asn Thr Val Leu Gly Met Gly Tyr Tyr Gly 65 70 75 80 Ser Ile Val Ala Asn Ser Ser Ser Ser Asn Asn Pro Ser Thr Leu Ala 85 90 95 Glu Asp Lys Leu Leu Val Leu Leu Ala Gln Leu Glu Ala Leu Thr Gln 100 105 110 Arg Leu Gly Glu Leu Ser Gln Gln Val Ala Gln Leu Arg Glu Gln Thr 115 120 125 Glu Ser Ala Val Ala Thr Ala Lys Ser Lys 130 135 <210> 74 <211> 389 <212> PRT <213> Simian adenovirus 29 <400> 74 Met His Pro Val Leu Arg Gln Met Arg Pro Gln Gln Gln Val Pro Ser 1 5 10 15 Gln Gln Gln Gln Gln Pro Gln Lys Ala Leu Pro Ala Pro Ala Pro Ala 20 25 30 Thr Thr Ala Val Ala Ala Val Cys Gly Ala Gly Gln Pro Ala Tyr Asp 35 40 45 Leu Asp Leu Glu Glu Gly Glu Gly Leu Ala Arg Leu Gly Ala Pro Ser 50 55 60 Pro Glu Arg His Pro Arg Val Gln Leu Lys Lys Asp Ser Arg Glu Ala 65 70 75 80 Tyr Val Pro Gln Gln Asn Leu Phe Arg Asp Arg Ser Gly Glu Glu Pro 85 90 95 Glu Glu Met Arg Ala Ser Arg Phe Asn Ala Gly Arg Glu Leu Arg His 100 105 110 Gly Leu Asp Arg Arg Arg Val Leu Arg Asp Glu Asp Phe Glu Val Asp 115 120 125 Glu Val Thr Gly Ile Ser Pro Ala Arg Ala His Val Ala Ala Ala Asn 130 135 140 Leu Val Ser Ala Tyr Glu Gln Thr Val Lys Glu Glu Arg Asn Phe Gln 145 150 155 160 Lys Ser Phe Asn Asn His Val Arg Thr Leu Ile Ala Arg Glu Glu Val 165 170 175 Thr Leu Gly Leu Met His Leu Trp Asp Leu Met Glu Ala Ile Thr Gln 180 185 190 Asn Pro Thr Ser Lys Pro Leu Thr Ala Gln Leu Phe Leu Val Val Gln 195 200 205 His Ser Arg Asp Asn Glu Ala Phe Arg Glu Ala Leu Leu Asn Ile Thr 210 215 220 Glu Pro Glu Gly Arg Trp Leu Tyr Asp Leu Ile Asn Ile Leu Gln Ser 225 230 235 240 Ile Ile Val Gln Glu Arg Ser Leu Gly Leu Ala Glu Lys Val Ala Ala 245 250 255 Ile Asn Tyr Ser Val Leu Ser Leu Gly Lys Tyr Tyr Ala Arg Lys Ile 260 265 270 Tyr Lys Thr Pro Tyr Val Pro Ile Asp Lys Glu Val Lys Ile Asp Gly 275 280 285 Phe Tyr Met Arg Met Thr Leu Lys Val Leu Thr Leu Ser Asp Asp Leu 290 295 300 Gly Val Tyr Arg Asn Asp Arg Met His Arg Ala Val Ser Ala Ser Arg 305 310 315 320 Arg Arg Glu Leu Ser Asp Arg Glu Leu Met His Ser Leu Gln Arg Ala 325 330 335 Leu Thr Gly Ala Gly Thr Asp Gly Glu Asn Tyr Phe Asp Met Gly Ala 340 345 350 Asp Leu Gln Trp Gln Pro Ser Arg Arg Thr Leu Asp Ala Ala Gly Cys 355 360 365 Glu Leu Pro Tyr Val Glu Glu Val Asp Glu Gly Glu Glu Glu Glu Gly 370 375 380 Glu Tyr Leu Glu Asp 385 <210> 75 <211> 587 <212> PRT <213> Simian adenovirus 29 <400> 75 Met Glu Gln Gln Ala Pro Asp Pro Ala Met Arg Ala Ala Leu Gln Ser 1 5 10 15 Gln Pro Ser Gly Ile Asn Ser Ser Asp Asp Trp Thr Gln Ala Met Gln 20 25 30 Arg Ile Met Ala Leu Thr Thr Arg Asn Pro Glu Ala Phe Arg Gln Gln 35 40 45 Pro Gln Ala Asn Arg Leu Ser Ala Ile Leu Glu Ala Val Val Pro Ser 50 55 60 Arg Ser Asn Pro Thr His Glu Lys Val Leu Ala Ile Val Asn Ala Leu 65 70 75 80 Val Glu Asn Lys Ala Ile Arg Pro Asp Glu Ala Gly Leu Val Tyr Asn 85 90 95 Ala Leu Leu Glu Arg Val Ala Arg Tyr Asn Ser Ser Asn Val Gln Thr 100 105 110 Asn Leu Asp Arg Met Val Thr Asp Val Arg Glu Ala Val Ser Gln Arg 115 120 125 Glu Arg Phe Gln Arg Asp Ala Asn Leu Gly Ser Leu Val Ala Leu Asn 130 135 140 Ala Phe Leu Ser Thr Gln Pro Ala Asn Val Pro Arg Gly Gln Gln Asp 145 150 155 160 Tyr Thr Asn Phe Leu Ser Ala Leu Arg Leu Met Val Thr Glu Val Pro 165 170 175 Gln Ser Glu Val Tyr Gln Ser Gly Pro Asp Tyr Phe Phe Gln Thr Ser 180 185 190 Arg Gln Gly Leu Gln Thr Val Asn Leu Ser Gln Ala Phe Lys Asn Leu 195 200 205 Lys Gly Leu Trp Gly Val His Ala Pro Val Gly Asp Arg Ala Thr Val 210 215 220 Ser Ser Leu Leu Thr Pro Asn Ser Arg Leu Leu Leu Leu Leu Val Ala 225 230 235 240 Pro Phe Thr Asp Ser Gly Ser Ile Asp Arg Asn Ser Tyr Leu Gly Tyr 245 250 255 Leu Leu Asn Leu Tyr Arg Glu Ala Ile Gly Gln Ser Gln Val Asp Glu 260 265 270 Gln Thr Tyr Gln Glu Ile Thr Gln Val Ser Arg Ala Leu Gly Gln Glu 275 280 285 Asp Thr Gly Ser Leu Glu Ala Thr Leu Asn Phe Leu Leu Thr Asn Arg 290 295 300 Ser Gln Lys Ile Pro Pro Gln Tyr Ala Leu Thr Ala Glu Glu Glu Arg 305 310 315 320 Ile Leu Arg Tyr Val Gln Gln Ser Val Gly Leu Phe Leu Met Gln Glu 325 330 335 Gly Ala Thr Pro Ser Ala Ala Leu Asp Met Thr Ala Arg Asn Met Glu 340 345 350 Pro Ser Met Tyr Ala Ser Asn Arg Pro Phe Ile Asn Lys Leu Leu Asp 355 360 365 Tyr Leu His Arg Ala Ala Ala Met Asn Ser Asp Tyr Phe Thr Asn Ala 370 375 380 Ile Leu Asn Pro His Trp Leu Pro Pro Pro Gly Phe Tyr Thr Gly Glu 385 390 395 400 Tyr Asp Met Pro Asp Pro Asn Asp Gly Phe Leu Trp Asp Asp Val Asp 405 410 415 Ser Ser Ile Phe Ser Pro Pro Pro Gly Tyr Asn Thr Trp Lys Lys Glu 420 425 430 Gly Gly Asp Arg Arg His Ser Ser Val Ser Leu Ser Gly Ser Arg Gly 435 440 445 Ala Ala Ala Ala Val Pro Glu Ala Ala Ser Pro Phe Pro Ser Leu Pro 450 455 460 Phe Ser Leu Asn Ser Val Arg Ser Ser Glu Leu Gly Arg Ile Thr Arg 465 470 475 480 Pro Arg Leu Met Gly Glu Asp Glu Tyr Leu Asn Asp Ser Leu Leu Arg 485 490 495 Pro Glu Arg Glu Lys Asn Phe Pro Asn Asn Gly Ile Glu Ser Leu Val 500 505 510 Asp Lys Met Ser Arg Trp Lys Thr Tyr Ala Gln Asp His Lys Asp Glu 515 520 525 Pro Arg Ile Leu Gly Ala Ala Ser Gly Thr Thr Arg Arg Arg Gln Arg 530 535 540 His Asp Arg Gln Arg Gly Leu Val Trp Asp Asp Glu Asp Ser Ala Asp 545 550 555 560 Asp Ser Ser Val Leu Asp Leu Gly Gly Arg Gly Gly Gly Asn Pro Phe 565 570 575 Ala His Leu Arg Pro His Phe Gly Arg Met Leu 580 585 <210> 76 <211> 576 <212> PRT <213> Simian adenovirus 29 <400> 76 Met Met Arg Arg Ala Val Leu Gly Gly Ala Val Val Tyr Pro Glu Gly 1 5 10 15 Pro Pro Pro Ser Tyr Glu Ser Val Met Gln Gln Gln Ala Ala Ala Val 20 25 30 Met Gln Pro Ser Leu Glu Ala Pro Phe Val Pro Pro Arg Tyr Leu Ala 35 40 45 Pro Thr Glu Gly Arg Asn Ser Ile Arg Tyr Ser Glu Leu Ala Pro Gln 50 55 60 Tyr Asp Thr Thr Arg Leu Tyr Leu Val Asp Asn Lys Ser Ala Asp Ile 65 70 75 80 Ala Ser Leu Asn Tyr Gln Asn Asp His Ser Asn Phe Leu Thr Thr Val 85 90 95 Val Gln Asn Asn Asp Phe Thr Pro Thr Glu Ala Ser Thr Gln Thr Ile 100 105 110 Asn Phe Asp Glu Arg Ser Arg Trp Gly Gly Gln Leu Lys Thr Ile Met 115 120 125 His Thr Asn Met Pro Asn Val Asn Glu Tyr Met Phe Ser Asn Lys Phe 130 135 140 Lys Ala Arg Val Met Val Ser Arg Glu Ala Ser Lys Ile Asp Ser Glu 145 150 155 160 Lys Asn Asp Arg Ser Lys Asp Thr Leu Lys Tyr Glu Trp Phe Glu Phe 165 170 175 Thr Leu Pro Glu Gly Asn Phe Ser Ala Thr Met Thr Ile Asp Leu Met 180 185 190 Asn Asn Ala Ile Ile Asp Asn Tyr Leu Ala Val Gly Arg Gln Asn Gly 195 200 205 Val Leu Gln Ser Asp Ile Gly Val Lys Phe Asp Thr Arg Asn Phe Arg 210 215 220 Leu Gly Trp Asp Pro Val Thr Lys Leu Val Met Pro Gly Val Tyr Thr 225 230 235 240 Tyr Glu Ala Phe His Pro Asp Ile Val Leu Leu Pro Asp Cys Gly Val 245 250 255 Asp Phe Thr Glu Ser Arg Leu Ser Asn Leu Leu Gly Ile Arg Lys Arg 260 265 270 His Pro Phe Gln Glu Gly Phe Lys Ile Met Tyr Glu Asp Leu Glu Gly 275 280 285 Gly Asn Ile Pro Ala Leu Leu Asp Val Ala Glu Tyr Glu Lys Ser Lys 290 295 300 Lys Glu Ile Ala Ser Ser Thr Thr Thr Thr Thr Ala Val Thr Thr Val 305 310 315 320 Ala Arg Asn Val Ala Asp Thr Ser Val Glu Ala Val Ala Val Ala Val 325 330 335 Val Asp Thr Ile Lys Ala Glu Asn Asp Ser Ala Val Arg Gly Asp Asn 340 345 350 Phe Gln Ser Lys Asn Asp Met Lys Ala Ser Glu Glu Val Thr Val Val 355 360 365 Pro Val Ser Pro Pro Thr Val Thr Glu Thr Glu Thr Lys Glu Pro Thr 370 375 380 Ile Lys Pro Leu Glu Lys Asp Thr Lys Asp Arg Ser Tyr Asn Val Ile 385 390 395 400 Ser Gly Thr Asn Asp Thr Ala Tyr Arg Ser Trp Tyr Leu Ala Tyr Asn 405 410 415 Tyr Gly Asp Pro Glu Lys Gly Val Arg Ser Trp Thr Leu Leu Thr Thr 420 425 430 Ser Asp Val Thr Cys Gly Ala Glu Gln Val Tyr Trp Ser Leu Pro Asp 435 440 445 Met Met Gln Asp Pro Val Thr Phe Arg Ser Thr Arg Gln Val Ser Asn 450 455 460 Tyr Pro Val Val Gly Ala Glu Leu Met Pro Val Phe Ser Lys Ser Phe 465 470 475 480 Tyr Asn Glu Gln Ala Val Tyr Ser Gln Gln Leu Arg Gln Thr Thr Ser 485 490 495 Leu Thr His Val Phe Asn Arg Phe Pro Glu Asn Gln Ile Leu Ile Arg 500 505 510 Pro Pro Ala Pro Thr Ile Thr Thr Val Ser Glu Asn Val Pro Ala Leu 515 520 525 Thr Asp His Gly Thr Leu Pro Leu Arg Ser Ser Ile Arg Gly Val Gln 530 535 540 Arg Val Thr Val Thr Asp Ala Arg Arg Arg Thr Cys Pro Tyr Val Tyr 545 550 555 560 Lys Ala Leu Gly Ile Val Ala Pro Arg Val Leu Ser Ser Arg Thr Phe 565 570 575 <210> 77 <211> 192 <212> PRT <213> Simian adenovirus 29 <400> 77 Met Ser Ile Leu Ile Ser Pro Ser Asn Asn Thr Gly Trp Gly Leu Arg 1 5 10 15 Thr Pro Thr Arg Met Tyr Gly Gly Ala Arg Lys Arg Ser Thr Gln His 20 25 30 Pro Val Arg Val Arg Gly His Phe Arg Ala Pro Trp Gly Ala Leu Lys 35 40 45 Gly Arg Ala Arg Thr Arg Thr Thr Val Asp Asp Val Ile Asp Gln Val 50 55 60 Val Ala Asp Ala Arg Asn Tyr Thr Pro Ala Ala Pro Ala Ser Thr Val 65 70 75 80 Asp Ala Val Ile Asp Ser Val Val Ala Asp Ala Arg Asp Tyr Ala Arg 85 90 95 Arg Lys Ser Arg Arg Arg Arg Ile Ala Arg Arg His Arg Ala Thr Pro 100 105 110 Ala Met Arg Ala Ala Arg Ala Leu Leu Arg Arg Ala Lys Arg Val Gly 115 120 125 Arg Arg Ala Met Leu Arg Ala Ala Arg Arg Ala Ala Ser Gly Ala Ser 130 135 140 Ala Gly Arg Ser Arg Arg Arg Ala Ala Thr Ala Ala Ala Ala Ala Ile 145 150 155 160 Ala Asn Met Ala Gln Pro Arg Arg Gly Asn Val Tyr Trp Val Arg Asp 165 170 175 Ala Thr Thr Gly Gln Arg Val Pro Val Arg Thr Arg Pro Pro Arg Thr 180 185 190 <210> 78 <211> 350 <212> PRT <213> Simian adenovirus 29 <400> 78 Met Ser Lys Arg Lys Tyr Lys Glu Glu Met Leu Gln Val Ile Ala Pro 1 5 10 15 Glu Ile Tyr Gly Pro Pro Val Lys Asp Glu Lys Lys Pro Arg Lys Ile 20 25 30 Lys Arg Val Lys Lys Asp Lys Lys Glu Glu Asp Gly Asp Asp Gly Leu 35 40 45 Val Glu Phe Val Arg Glu Phe Ala Pro Arg Arg Arg Val Gln Trp Arg 50 55 60 Gly Arg Arg Val Arg Pro Val Leu Arg Pro Gly Thr Thr Val Val Phe 65 70 75 80 Thr Pro Gly Glu Arg Ser Ser Thr Thr Phe Lys Arg Ser Tyr Asp Glu 85 90 95 Val Tyr Gly Asp Asp Asp Ile Leu Glu Gln Ala Ala Asp Arg Leu Gly 100 105 110 Glu Phe Ala Tyr Gly Lys Arg Ser Arg Ser Ser Pro Lys Asp Glu Ala 115 120 125 Val Ser Ile Pro Leu Asp His Gly Asn Pro Thr Pro Ser Leu Lys Pro 130 135 140 Val Thr Leu Gln Gln Val Leu Pro Val Pro Pro Arg Arg Gly Val Lys 145 150 155 160 Arg Glu Gly Glu Asp Leu Tyr Pro Thr Met Gln Leu Met Val Pro Lys 165 170 175 Arg Gln Lys Leu Glu Asp Val Leu Glu Lys Met Lys Val Asp Pro Asp 180 185 190 Ile Gln Pro Glu Val Lys Val Arg Pro Ile Lys Gln Val Ala Pro Gly 195 200 205 Leu Gly Val Gln Thr Val Asp Ile Lys Ile Pro Thr Glu Ser Met Glu 210 215 220 Val Gln Thr Glu Pro Ala Lys Pro Thr Ala Thr Ser Ile Glu Val Gln 225 230 235 240 Thr Asp Pro Trp Met Pro Ala Pro Ile Ala Thr Thr Ala Ser Thr Ala 245 250 255 Arg Arg Pro Arg Arg Lys Tyr Gly Pro Ala Ser Leu Leu Met Pro Asn 260 265 270 Tyr Ala Leu His Pro Ser Ile Ile Pro Thr Pro Gly Tyr Arg Gly Thr 275 280 285 Arg Tyr Tyr Arg Ser Arg Ser Thr Thr Ser Arg Arg Arg Lys Thr Pro 290 295 300 Ala Ser Arg Ser Arg Arg Arg Arg Arg Arg Thr Ala Ser Lys Leu Thr 305 310 315 320 Pro Ala Ala Leu Val Arg Arg Val Tyr Arg Asp Gly Arg Ala Glu Pro 325 330 335 Leu Met Leu Pro Arg Ala Arg Tyr His Pro Ser Ile Thr Thr 340 345 350 <210> 79 <211> 75 <212> PRT <213> Simian adenovirus 29 <400> 79 Met Ala Leu Thr Cys Arg Leu Arg Val Pro Ile Thr Gly Tyr Arg Gly 1 5 10 15 Arg Asn Ser Arg Arg Arg Arg Met Leu Gly Arg Gly Met Arg Arg His 20 25 30 Arg Arg Arg Arg Ala Ile Ser Lys Arg Leu Gly Gly Gly Phe Leu Thr 35 40 45 Ala Leu Ile Pro Ile Ile Ala Ala Ala Ile Gly Ala Val Pro Gly Ile 50 55 60 Ala Ser Val Ala Val Gln Ala Ser Gln Arg His 65 70 75 <210> 80 <211> 250 <212> PRT <213> Simian adenovirus 29 <400> 80 Met Glu Asp Ile Asn Phe Ser Ser Leu Ala Pro Arg His Gly Thr Arg 1 5 10 15 Pro Tyr Met Gly Thr Trp Ser Asp Ile Gly Thr Ser Gln Leu Asn Gly 20 25 30 Gly Ala Phe Asn Trp Ser Ser Ile Trp Ser Gly Leu Lys Asn Phe Gly 35 40 45 Ser Thr Ile Lys Thr Tyr Gly Asn Lys Ala Trp Asn Ser Ser Thr Gly 50 55 60 Gln Ala Leu Arg Asn Lys Leu Lys Glu Gln Asn Phe Gln Gln Lys Val 65 70 75 80 Val Asp Gly Ile Ala Ser Gly Ile Asn Gly Val Val Asp Leu Ala Asn 85 90 95 Gln Ala Val Gln Lys Gln Ile Asn Ser Arg Leu Asp Ala Pro Pro Ala 100 105 110 Ala Pro Gly Glu Met Glu Val Glu Glu Glu Leu Pro Pro Leu Glu Lys 115 120 125 Arg Gly Asp Lys Arg Pro Arg Pro Asp Met Glu Glu Thr Leu Val Thr 130 135 140 Arg Gly Asp Glu Pro Pro Pro Tyr Glu Glu Ala Ile Lys Leu Gly Met 145 150 155 160 Pro Thr Thr Arg Pro Ile Ala Pro Met Ala Thr Gly Val Met Lys Pro 165 170 175 Ser Gln Ser His Arg Pro Ala Thr Leu Asp Leu Pro Pro Ser Pro Ala 180 185 190 Ala Val Ala Ser Ala Pro Lys Pro Val Ala Thr Pro Lys Pro Thr Thr 195 200 205 Val Gln Pro Val Ala Val Ala Arg Pro Arg Pro Gly Gly Thr Pro Arg 210 215 220 Pro Asn Ala Asn Trp Gln Ser Thr Leu Asn Ser Ile Val Gly Leu Gly 225 230 235 240 Val Gln Ser Val Lys Arg Arg Arg Cys Tyr 245 250 <210> 81 <211> 954 <212> PRT <213> Simian adenovirus 29 <400> 81 Met Ala Thr Pro Ser Met Leu Pro Gln Trp Ala Tyr Met His Ile Ala 1 5 10 15 Gly Gln Asp Ala Ser Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala 20 25 30 Arg Ala Thr Asp Thr Tyr Phe Asn Leu Gly Asn Lys Phe Arg Asn Pro 35 40 45 Thr Val Ala Pro Thr His Asp Val Thr Thr Asp Arg Ser Gln Arg Leu 50 55 60 Met Leu Arg Phe Val Pro Val Asp Arg Glu Asp Asn Thr Tyr Ser Tyr 65 70 75 80 Lys Val Arg Tyr Thr Leu Ala Val Gly Asp Asn Arg Val Leu Asp Met 85 90 95 Ala Ser Thr Phe Phe Asp Ile Arg Gly Val Leu Asp Arg Gly Pro Ser 100 105 110 Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser Leu Ala Pro Lys Gly 115 120 125 Ala Pro Asn Thr Ser Gln Trp Val Asp Glu Gly Lys Lys Ile Thr Asp 130 135 140 Asn Gly Val Glu Ala Ala Asp Asp Asn Ala Lys Ala Thr Tyr Thr Phe 145 150 155 160 Gly Asn Ala Pro Val Lys Ala Glu Asp Asp Ile Thr Lys Asp Gly Leu 165 170 175 Pro Val Ala Val Glu Val Thr Gly Glu Asp Asp Glu Thr Lys Pro Ile 180 185 190 Tyr Ala Asp Lys Leu Tyr Gln Pro Glu Pro Gln Val Gly Glu Glu Thr 195 200 205 Trp Thr Asp Thr Asp Ala Thr Thr Glu Lys Tyr Gly Gly Arg Ala Leu 210 215 220 Lys Pro Asp Thr Lys Met Lys Pro Cys Tyr Gly Ser Phe Ala Lys Pro 225 230 235 240 Thr Asn Thr Lys Gly Gly Gln Ala Lys Val Lys Lys Thr Glu Glu Glu 245 250 255 Thr Val Asp Pro Thr Lys Val Gln Tyr Asp Ile Asp Met Asn Phe Phe 260 265 270 Glu Glu Arg Ser Gln Lys Asn Gly Ser Pro Lys Ile Val Met Tyr Ala 275 280 285 Glu Asn Val Asp Leu Glu Thr Pro Asp Thr His Val Val Tyr Lys Pro 290 295 300 Gly Thr Ser Glu Asp Ser Ser His Ala Asn Leu Gly Gln Gln Ser Met 305 310 315 320 Pro Asn Arg Pro Asn Tyr Ile Gly Phe Arg Asp Asn Phe Ile Gly Leu 325 330 335 Met Tyr Tyr Asn Ser Thr Gly Asn Met Gly Val Leu Ala Gly Gln Ala 340 345 350 Ser Gln Leu Asn Ala Val Val Asp Leu Gln Asp Arg Asn Thr Glu Leu 355 360 365 Ser Tyr Gln Leu Leu Leu Asp Ser Leu Gly Asp Arg Thr Arg Tyr Phe 370 375 380 Ser Met Trp Asn Gln Ala Val Asp Ser Tyr Asp Pro Asp Val Arg Ile 385 390 395 400 Ile Glu Asn His Gly Val Glu Asp Glu Leu Pro Asn Tyr Cys Phe Pro 405 410 415 Leu Asp Gly Val Gly Pro Arg Thr Val Ser Tyr Lys Gln Met Glu Pro 420 425 430 Asn Gly Thr Asp Ala Ala Asp Ala Asn Gly Trp Lys Asn Val Thr Pro 435 440 445 Thr Gly Ile Ser Glu Ile Ala Lys Gly Asn Pro Phe Ala Met Glu Ile 450 455 460 Asn Leu Gln Ala Asn Leu Trp Arg Ser Phe Leu Tyr Ser Asn Val Ala 465 470 475 480 Leu Tyr Leu Pro Asp Ser Tyr Lys Tyr Thr Pro Ala Asn Val Thr Leu 485 490 495 Pro Thr Asn Thr Asn Thr Tyr Asp Tyr Leu Asn Gly Arg Val Val Pro 500 505 510 Pro Ser Leu Val Asp Thr Tyr Val Asn Ile Gly Ala Arg Trp Ser Leu 515 520 525 Asp Ala Met Asp Asn Val Asn Pro Phe Asn His His Arg Asn Ala Gly 530 535 540 Leu Arg Tyr Arg Ser Met Leu Leu Gly Asn Gly Arg Tyr Val Pro Phe 545 550 555 560 His Ile Gln Val Pro Gln Lys Phe Phe Ala Ile Lys Asn Leu Leu Leu 565 570 575 Leu Pro Gly Ser Tyr Thr Tyr Glu Trp Asn Phe Arg Lys Asp Val Asn 580 585 590 Met Val Leu Gln Ser Ser Leu Gly Asn Asp Leu Arg Thr Asp Gly Ala 595 600 605 Ser Ile Ser Phe Thr Ser Ile Asn Leu Tyr Ala Thr Phe Phe Pro Met 610 615 620 Ala His Asn Thr Ala Ser Thr Leu Glu Ala Met Leu Arg Asn Asp Thr 625 630 635 640 Asn Asp Gln Ser Phe Asn Asp Tyr Leu Ser Ala Ala Asn Met Leu Tyr 645 650 655 Pro Ile Pro Ala Asn Ala Thr Asn Ile Pro Ile Ser Ile Pro Ser Arg 660 665 670 Asn Trp Ala Ala Phe Arg Gly Trp Ser Phe Thr Arg Leu Lys Thr Lys 675 680 685 Glu Thr Pro Ser Leu Gly Ser Gly Phe Asp Pro Tyr Phe Val Tyr Ser 690 695 700 Gly Ser Ile Pro Tyr Leu Asp Gly Thr Phe Tyr Leu Asn His Thr Phe 705 710 715 720 Lys Lys Val Ser Ile Met Phe Asp Ser Ser Val Ser Trp Pro Gly Asn 725 730 735 Asp Arg Leu Leu Thr Pro Asn Glu Phe Glu Ile Lys Arg Thr Val Asp 740 745 750 Gly Glu Gly Tyr Asn Val Ala Gln Cys Asn Met Thr Lys Asp Trp Phe 755 760 765 Leu Val Gln Met Leu Ala Asn Tyr Asn Ile Gly Tyr Gln Gly Phe Tyr 770 775 780 Ile Pro Glu Gly Tyr Lys Asp Arg Met Tyr Ser Phe Phe Arg Asn Phe 785 790 795 800 Gln Pro Met Ser Arg Gln Val Val Asp Glu Ile Asn Tyr Lys Asp Tyr 805 810 815 Lys Ala Val Ala Val Pro Tyr Gln His Asn Asn Ser Gly Phe Val Gly 820 825 830 Tyr Met Ala Pro Thr Met Arg Gln Gly Gln Ala Tyr Pro Ala Asn Tyr 835 840 845 Pro Tyr Pro Leu Ile Gly Thr Thr Ala Val Lys Ser Val Thr Gln Lys 850 855 860 Lys Phe Leu Cys Asp Arg Thr Met Trp Arg Ile Pro Phe Ser Ser Asn 865 870 875 880 Phe Met Ser Met Gly Ala Leu Thr Asp Leu Gly Gln Asn Met Leu Tyr 885 890 895 Ala Asn Ser Ala His Ala Leu Asp Met Thr Phe Glu Val Asp Pro Met 900 905 910 Asp Glu Pro Thr Leu Leu Tyr Leu Leu Phe Glu Val Phe Asp Val Val 915 920 925 Arg Val His Gln Pro His Arg Gly Val Ile Glu Ala Val Tyr Leu Arg 930 935 940 Thr Pro Phe Ser Ala Gly Asn Ala Thr Thr 945 950 <210> 82 <211> 209 <212> PRT <213> Simian adenovirus 29 <400> 82 Met Ala Cys Gly Ser Gly Asn Gly Ser Ser Glu Gln Glu Leu Arg Ala 1 5 10 15 Ile Ala Arg Asp Leu Gly Cys Gly Pro Tyr Phe Leu Gly Thr Phe Asp 20 25 30 Lys Arg Phe Pro Gly Phe Met Ala Pro Asp Lys Leu Ala Cys Ala Ile 35 40 45 Val Asn Thr Ala Gly Arg Glu Thr Gly Gly Glu His Trp Leu Ala Phe 50 55 60 Gly Trp Asn Pro Arg Ser Asn Thr Cys Tyr Leu Phe Asp Pro Phe Gly 65 70 75 80 Phe Ser Asp Glu Arg Leu Lys Gln Ile Tyr Gln Phe Glu Tyr Glu Gly 85 90 95 Leu Leu Arg Arg Ser Ala Leu Ala Thr Lys Asp Arg Cys Ile Thr Leu 100 105 110 Glu Lys Ser Thr Gln Thr Val Gln Gly Pro Arg Ser Ala Ala Cys Gly 115 120 125 Leu Phe Cys Cys Met Phe Leu His Ala Phe Val His Trp Pro Asp Arg 130 135 140 Pro Met Asp Gly Asn Pro Thr Met Lys Leu Leu Thr Gly Val Pro Asn 145 150 155 160 Ser Met Leu Gln Ser Pro Gln Val Gln Pro Thr Leu Arg His Asn Gln 165 170 175 Glu Ala Leu Tyr Arg Phe Leu Asn Thr His Ser Ser Tyr Phe Arg Ser 180 185 190 His Arg Ala Arg Ile Glu Lys Ala Thr Ala Phe Asp Arg Met Asp Met 195 200 205 Gln <210> 83 <211> 832 <212> PRT <213> Simian adenovirus 29 <400> 83 Met Glu Thr Gln Pro Ser Leu Pro Thr Pro Leu Gln Ala Pro Ser His 1 5 10 15 Leu Ala Pro Ser Ser Asp Glu Glu Glu Gln Ser Leu Thr Thr Pro Pro 20 25 30 Pro Ser Pro Ala Thr Thr Thr Ser Thr Leu Glu Asp Glu Glu Glu Val 35 40 45 Asp Ala Pro Gln Glu Met Gln Asp Met Glu Asp Glu Lys Ala Glu Glu 50 55 60 Ile Glu Ala Asp Val Glu Gln Asp Pro Gly Tyr Val Thr Pro Ala Glu 65 70 75 80 His Glu Glu Glu Leu Arg Arg Phe Leu Asp Arg Glu Asp Asn Asn Arg 85 90 95 Pro Glu Gln Gln Ala Asp Gly Asp His Gln Glu Ala Gly Leu Gly Asp 100 105 110 His Val Ala Asp Tyr Leu Thr Gly Leu Gly Gly Glu Asp Val Leu Leu 115 120 125 Lys His Leu Ala Arg Gln Ser Ile Ile Val Lys Asp Ala Leu Leu Gly 130 135 140 Arg Thr Glu Val Pro Ile Ser Val Glu Glu Leu Ser Arg Ala Tyr Glu 145 150 155 160 Leu Asn Leu Phe Ser Pro Arg Val Pro Pro Lys Arg Gln Pro Asn Gly 165 170 175 Thr Cys Glu Pro Asn Pro Arg Leu Asn Phe Tyr Pro Ala Phe Ala Val 180 185 190 Pro Glu Val Leu Ala Thr Tyr His Ile Phe Phe Lys Asn Gln Lys Ile 195 200 205 Pro Val Ser Cys Arg Ala Asn Arg Thr Arg Ala Asp Ala Leu Leu Asn 210 215 220 Leu Gly Pro Gly Ala Arg Leu Pro Asp Ile Ala Ser Leu Glu Glu Val 225 230 235 240 Pro Lys Ile Phe Glu Gly Leu Gly Ser Asp Glu Thr Arg Ala Ala Asn 245 250 255 Ala Leu Gln Gln Gly Glu Asn Gly Met Asp Glu His His Ser Ala Leu 260 265 270 Val Glu Leu Glu Gly Asp Asn Ala Arg Leu Ala Val Leu Lys Arg Ser 275 280 285 Ile Glu Val Thr His Phe Ala Tyr Pro Ala Val Asn Leu Pro Pro Lys 290 295 300 Val Met Ser Ala Val Met Asp Gln Leu Leu Ile Lys Arg Ala Ser Pro 305 310 315 320 Leu Ser Glu Asp Gln Asn Met Gln Asp Pro Asp Ala Ser Asp Glu Gly 325 330 335 Lys Pro Val Val Ser Asp Glu Gln Leu Ser Arg Trp Leu Gly Thr Asn 340 345 350 Ser Pro Arg Asp Leu Glu Glu Arg Arg Lys Leu Met Met Ala Val Val 355 360 365 Leu Val Thr Val Glu Leu Glu Cys Leu Arg Arg Phe Phe Thr Asp Pro 370 375 380 Glu Thr Leu Arg Lys Leu Glu Glu Asn Leu His Tyr Thr Phe Arg His 385 390 395 400 Gly Phe Val Arg Gln Ala Cys Lys Ile Ser Asn Val Glu Leu Thr Asn 405 410 415 Leu Val Ser Tyr Met Gly Ile Leu His Glu Asn Arg Leu Gly Gln Ser 420 425 430 Val Leu His Thr Thr Leu Lys Gly Glu Ala Arg Arg Asp Tyr Ile Arg 435 440 445 Asp Cys Val Tyr Leu Tyr Leu Cys His Thr Trp Gln Thr Gly Met Gly 450 455 460 Val Trp Gln Gln Cys Leu Glu Glu Gln Asn Leu Lys Glu Leu Asp Lys 465 470 475 480 Leu Leu Gln Arg Ser Leu Lys Ala Leu Trp Thr Gly Phe Asp Glu Arg 485 490 495 Thr Val Ala Ser Asp Leu Ala Asp Ile Ile Phe Pro Glu Arg Leu Arg 500 505 510 Val Thr Leu Arg Asn Gly Leu Pro Asp Phe Met Ser Gln Ser Met Leu 515 520 525 Asn Asn Phe Arg Ser Phe Ile Leu Glu Arg Ser Gly Ile Leu Pro Ala 530 535 540 Thr Cys Cys Ala Leu Pro Ser Asp Phe Val Pro Leu Thr Tyr Arg Glu 545 550 555 560 Cys Pro Pro Pro Leu Trp Ser His Cys Tyr Leu Phe Arg Leu Ala Asn 565 570 575 Tyr Leu Ser Tyr His Ser Asp Val Ile Glu Asp Val Ser Gly Asp Gly 580 585 590 Leu Leu Glu Cys His Cys Arg Cys Asn Leu Cys Thr Pro His Arg Ser 595 600 605 Leu Ala Cys Asn Pro Gln Leu Leu Ser Glu Thr Gln Ile Ile Gly Thr 610 615 620 Phe Glu Leu Gln Gly Pro Ser Ser Glu Gly Glu Gly Ser Ser Pro Gly 625 630 635 640 Gln Ser Leu Lys Leu Thr Pro Gly Leu Trp Thr Ser Ala Tyr Leu Arg 645 650 655 Lys Phe Ala Pro Glu Asp Tyr His Pro Tyr Glu Ile Arg Phe Tyr Glu 660 665 670 Asp Gln Ser Gln Pro Pro Lys Ala Glu Leu Ser Ala Cys Val Ile Thr 675 680 685 Gln Gly Ala Ile Leu Ala Gln Leu Gln Ala Ile Gln Lys Ser Arg Gln 690 695 700 Glu Phe Leu Leu Lys Arg Gly Asn Gly Val Tyr Leu Asp Pro Gln Thr 705 710 715 720 Gly Glu Glu Leu Asn Thr Arg Phe Pro Gln Asp Val Pro Ala Pro Arg 725 730 735 Lys Gln Glu Val Glu Ser Ala Ala Ala Ala Pro Arg Gly His Gly Gly 740 745 750 Arg Leu Gly Gln Ser Gly Arg Gly Gly Gly Asp Gly Arg Leu Gly Gln 755 760 765 Pro Gly Arg Gly Gly Gly Gln Pro Gly Gly Arg Gln Phe Gly Gly Gly 770 775 780 Arg Arg Gly Gly Arg Gly Gly Gly Arg Ser Ser Arg Arg Gln Thr Val 785 790 795 800 Val Leu Gly Ser Gly Asp Lys Gln Gly Pro Arg Gln Gln Gln Gln His 805 810 815 Gly Tyr Asn Leu Arg Ser Gly Ser Gly Gly Pro Ala Ala Ser Gln Gln 820 825 830 <210> 84 <211> 227 <212> PRT <213> Simian adenovirus 29 <400> 84 Met Ser Lys Glu Ile Pro Thr Pro Tyr Met Trp Ser Tyr Gln Pro Gln 1 5 10 15 Met Gly Leu Ala Ala Gly Ala Ser Gln Asp Tyr Ser Thr Arg Met Asn 20 25 30 Trp Leu Ser Ala Gly Pro Ser Met Ile Ser Arg Val Asn Asp Ile Arg 35 40 45 Ala Tyr Arg Asn Gln Leu Leu Leu Glu Gln Ser Ala Leu Thr Thr Thr 50 55 60 Pro Arg Gln His Leu Asn Pro Arg Asn Trp Pro Ala Ala Leu Val Tyr 65 70 75 80 Gln Glu Thr Pro Ala Pro Thr Thr Val Leu Leu Pro Arg Asp Ala Gln 85 90 95 Ala Glu Val Gln Met Thr Asn Ala Gly Val Gln Leu Ala Gly Gly Ser 100 105 110 Ala Leu Cys Arg His Arg Pro Gln Gln Ser Ile Lys Arg Leu Val Ile 115 120 125 Arg Gly Arg Gly Ile Gln Leu Asn Asp Glu Ser Val Ser Ser Ser Leu 130 135 140 Gly Leu Arg Pro Asp Gly Val Phe Gln Ile Ala Gly Cys Gly Arg Ser 145 150 155 160 Ser Phe Thr Pro Arg Gln Ala Val Leu Thr Leu Glu Ser Ser Ser Ser 165 170 175 Gln Pro Arg Ser Gly Gly Ile Gly Thr Leu Gln Phe Val Glu Glu Phe 180 185 190 Thr Pro Ser Val Tyr Phe Asn Pro Phe Ser Gly Ser Pro Gly Gln Tyr 195 200 205 Pro Asp Glu Phe Ile Pro Asn Phe Asp Ala Ile Ser Glu Ser Val Asp 210 215 220 Gly Tyr Asp 225 <210> 85 <211> 148 <212> PRT <213> Simian adenovirus 29 <400> 85 Met Lys Ala Phe Ala Val Leu Phe Val Leu Ser Leu Ile Lys Thr Glu 1 5 10 15 Leu Arg Leu Thr Phe Gly Leu Pro Leu Leu Gln Pro Gly Leu Tyr Asn 20 25 30 Thr Ser Gln Thr Leu Arg Ser Ser Gln Lys Asn Gln Thr Leu Pro Leu 35 40 45 Ile Gln Asp Ser Asn Ser Thr Ser Pro Ala Pro Phe Pro Thr Asn Leu 50 55 60 Pro Val Thr Asn Asn Leu Gly Ala Gln Leu His His Arg Phe Ser Arg 65 70 75 80 Ser Leu Leu Ser Ala Asn Ile Thr Thr Pro Arg Thr Gly Gly Glu Leu 85 90 95 Arg Gly Leu Pro Thr Asp Asn Pro Trp Val Val Ala Gly Phe Val Ala 100 105 110 Leu Gly Val Val Ala Gly Gly Leu Val Leu Ile Leu Cys Tyr Leu Tyr 115 120 125 Thr Pro Cys Cys Ala Tyr Leu Val Val Leu Cys Cys Trp Phe Lys Lys 130 135 140 Trp Gly Ser Tyr 145 <210> 86 <211> 246 <212> PRT <213> Simian adenovirus 29 <400> 86 Met Ala Ser Gln Gln Arg Glu His Cys Gly Phe Leu His Cys Leu Leu 1 5 10 15 Leu Val Tyr Leu His His His Cys Tyr His Val Cys Glu His Thr Leu 20 25 30 Ala Tyr Ser His Ser Pro Lys Lys Gln Ser Arg Lys Arg Glu Asn Ala 35 40 45 Leu Ile Ile Asn Phe Tyr Leu Gln Lys Met Thr Ser Val Ser Ala Leu 50 55 60 Ile Phe Ala Thr Ile Met Ala Val Gln Gly Gln Ala Ala Gln Gly Gln 65 70 75 80 Thr Leu Ile Asn Val His Pro Gly Thr Asn His Thr Leu Val Val Pro 85 90 95 Asn Asn Tyr Ser Asn Ile Glu Trp Gln Trp Phe Thr Asn Asn Val Trp 100 105 110 Tyr Glu Pro Cys Glu His Tyr Ser Leu Phe Ile Cys Asn His Asn Leu 115 120 125 Thr Leu Ile Asn Val Ser Thr Ile His Lys Gly Tyr Tyr Tyr Arg Tyr 130 135 140 Asp Asn His Ser Ile Asp Pro Thr Ile Tyr Leu Val Arg Val Asn Pro 145 150 155 160 Ile Asn Lys Pro Ile Pro Lys Ala Phe Ser Arg Thr Thr Ile Gln Asn 165 170 175 Phe Lys Thr Ala Ile Leu Leu Asn Phe Lys Thr Lys Asn Ile Thr Gly 180 185 190 Asn Ile Leu Pro Thr Thr Pro Thr Glu Lys Asn Thr Pro Asn Ser Ile 195 200 205 Phe Glu Ile Ile Ile Ala Leu Leu Ala Val Gly Ile Thr Ile Ile Leu 210 215 220 Cys Met Ile Ile Tyr Ala His Cys Tyr Lys Lys Ile His His Lys Lys 225 230 235 240 Glu Pro Leu Leu Ser Phe 245 <210> 87 <211> 188 <212> PRT <213> Simian adenovirus 29 <400> 87 Met Ile Phe Phe Ala Thr Leu Ile Thr Ile Gly Ile Val Gln Gly Gln 1 5 10 15 Asp Ile Thr Ile Gly Tyr Val Gly Asn Asn Ile Thr Leu Leu Gly Pro 20 25 30 Pro Thr Gly Thr Ile Pro Thr Trp Tyr Lys Ile Tyr Glu Arg Gly Trp 35 40 45 Trp Ile Arg Pro Cys Asp Gln Gly Gly Ser Lys Tyr Ile Cys Gly Arg 50 55 60 Asp Ile Thr Ile Thr Asn Leu Asn Lys Asn Asp Asn Gly Tyr Tyr Phe 65 70 75 80 Cys Asn Asn Tyr Gly Gly Gly Lys Lys Ser Tyr Thr Leu Glu Val Arg 85 90 95 Asp Pro Thr Thr Leu Ala Pro His Thr Thr Phe Ser Ser Ser Thr Ser 100 105 110 Arg Asn Thr His Glu Ala Ala Tyr Ala Arg Ala Met Leu Gln Lys Ile 115 120 125 Asn Glu Thr Ile Asn Ser Thr Ile Ser His Asn Pro Asp Glu Ile Pro 130 135 140 Lys Ser Met Ile Gly Ile Ile Val Ala Val Ala Val Gly Met Ala Ile 145 150 155 160 Ile Ile Ile Cys Met Ile Val Tyr Ala Cys Cys Tyr Arg Lys Phe Gln 165 170 175 Asp Glu Lys Gly Asp Pro Leu Leu Ser Phe Asp Ile 180 185 <210> 88 <211> 107 <212> PRT <213> Simian adenovirus 29 <400> 88 Met Lys Gly Val Gly Ile Leu Val Leu Ser Thr Leu Ile Tyr Ser Val 1 5 10 15 Ile Pro Ile Ser Ile Asn Val Gln Thr Thr Leu Asn Glu Thr Gly Asn 20 25 30 His Ser Thr Thr Ser His Thr Pro Pro Pro Leu Ser Thr His Pro Gln 35 40 45 Ser Lys Asp Ala Ile Gln Leu Gln Leu Thr Ile Leu Ile Val Ile Gly 50 55 60 Leu Thr Ile Leu Ala Val Ile Leu Tyr Phe Ile Phe Cys Arg Gln Ile 65 70 75 80 Pro Asn Val Val Lys Lys Pro Thr Arg Arg Pro Ile Tyr Arg Ser Ile 85 90 95 Ile Ser Lys Pro His Met Ala Leu Asn Glu Ile 100 105 <210> 89 <211> 91 <212> PRT <213> Simian adenovirus 29 <400> 89 Met Ile Pro Arg Asn Phe Phe Phe Thr Ile Leu Ile Cys Ala Phe Asn 1 5 10 15 Val Cys Ala Thr Phe Ala Thr Val Ala Asn Val Thr Pro Asp Cys Ile 20 25 30 Gly Ala Phe Ala Ser Tyr Val Leu Phe Ala Phe Ile Thr Cys Ile Cys 35 40 45 Val Cys Ser Ile Val Cys Leu Val Ile Asn Phe Phe Gln Leu Val Asp 50 55 60 Trp Val Phe Val Arg Ile Ala Tyr Leu Arg His His Pro Glu Tyr Arg 65 70 75 80 Asn Gln Asn Val Ala Ala Ile Leu Arg Leu Ile 85 90 <210> 90 <211> 132 <212> PRT <213> Simian adenovirus 29 <400> 90 Met Thr Asp Pro His Ala Ala Ala Glu Glu Leu Leu Asp Met Asp Gly 1 5 10 15 Arg Ala Ser Glu Gln Arg Leu Ala Gln Leu Arg Ile Arg Gln Gln Gln 20 25 30 Glu Arg Ala Ala Lys Glu Leu Arg Asp Ala Ile Glu Ile His Gln Cys 35 40 45 Lys Lys Gly Ile Phe Cys Leu Val Lys Gln Ala Lys Ile Ser Tyr Glu 50 55 60 Ile Thr Ala Asn Asp His Arg Leu Ser Tyr Glu Leu Gly Pro Gln Arg 65 70 75 80 Gln Lys Phe Thr Cys Met Val Gly Ile Asn Pro Ile Val Ile Thr Gln 85 90 95 Gln Ala Gly Asp Thr Lys Gly Cys Ile His Cys Ser Cys Glu Ser Thr 100 105 110 Glu Cys Ile Tyr Thr Leu Leu Lys Thr Leu Cys Gly Leu Arg Asp Leu 115 120 125 Leu Pro Met Asn 130 <210> 91 <211> 324 <212> PRT <213> Simian adenovirus 29 <400> 91 Met Ala Lys Arg Ala Arg Leu Ser Ser Ser Phe Asn Pro Val Tyr Pro 1 5 10 15 Tyr Glu Asp Glu Ser Ser Ser Gln His Pro Phe Ile Asn Pro Gly Phe 20 25 30 Ile Ser Pro Asn Gly Phe Thr Gln Ser Pro Asp Gly Ala Leu Thr Leu 35 40 45 Lys Cys Val Ala Pro Leu Thr Thr Thr Ser Gly Ser Leu Asp Ile Lys 50 55 60 Val Gly Gly Gly Leu Lys Val Asp Ser Thr Asp Gly Ser Leu Glu Glu 65 70 75 80 Asn Ile Ser Thr Thr Ala Pro Leu Asn Lys Ser Asn His Ser Ile Gly 85 90 95 Leu Ala Val Gly Asn Gly Leu Gln Thr Asn Glu Ser Lys Leu Cys Ala 100 105 110 Lys Leu Gly Glu Glu Leu Thr Phe Asp Ser Ser Asn Ala Ile Thr Ile 115 120 125 Lys Asn Asn Thr Leu Trp Thr Gly Ala Lys Pro Ser Thr Asn Cys Lys 130 135 140 Ile Gln Glu Asp Ala Asp Ala Leu Asp Cys Lys Leu Thr Leu Val Leu 145 150 155 160 Val Lys Asn Gly Gly Leu Val Asn Ala Tyr Val Ser Leu Ile Gly Asp 165 170 175 Ser Asp Tyr Val Asn Thr Leu Phe Thr Lys Lys Thr Ala Ser Ile Ser 180 185 190 Val Glu Leu Ala Phe Asp Ser Ser Gly Gln Ile Leu Thr Ser Leu Ser 195 200 205 Ser Leu Lys Thr Ser Leu Asn Phe Lys His Asn Gln Asp Met Ala Thr 210 215 220 Glu Thr Ile Ser Ala Lys Gly Phe Met Pro Ser Thr Thr Ala Tyr Pro 225 230 235 240 Phe Asn Thr Gln Ala Thr Ser Ser Arg Asp Asn Glu Asp Tyr Ile Phe 245 250 255 Gly Lys Cys Tyr Tyr Arg Ala Ser Tyr Gly Ala Leu Tyr Thr Leu Asp 260 265 270 Val Thr Val Ile Leu Asn Arg Arg Met Thr Ala Ala Gly Met Ala Tyr 275 280 285 Ala Met Asn Phe Thr Trp Leu Leu Asp Ala Thr Asp Ala Pro Glu Asn 290 295 300 Thr Thr Thr Thr Leu Val Thr Ser Pro Phe Ser Phe Ser Tyr Ile Arg 305 310 315 320 Glu Asp Asp Asp <210> 92 <211> 550 <212> DNA <213> Simian adenovirus 29 <220> <221> CDS <222> (5)..(547) <223> label=Elb\19K <400> 92 atcc atg gag gtt tgg gct atc ttg gaa gat ctc agg cag act aga caa 49 Met Glu Val Trp Ala Ile Leu Glu Asp Leu Arg Gln Thr Arg Gln 1 5 10 15 ctg cta gaa aac gcc tcg gac gga gtc tct agt ctt tgg aga ttc tgg 97 Leu Leu Glu Asn Ala Ser Asp Gly Val Ser Ser Leu Trp Arg Phe Trp 20 25 30 ttc ggt ggt gat cta gct agg cta gtc ttt agg gta aaa cgg gag tat 145 Phe Gly Gly Asp Leu Ala Arg Leu Val Phe Arg Val Lys Arg Glu Tyr 35 40 45 agt gaa gaa ttt gaa aag tta ttg gaa gac agt cca gga ctt ttt gaa 193 Ser Glu Glu Phe Glu Lys Leu Leu Glu Asp Ser Pro Gly Leu Phe Glu 50 55 60 gcc ctt aac ttg ggc cac cag gct cat ttt aag gag aag gtt tta tca 241 Ala Leu Asn Leu Gly His Gln Ala His Phe Lys Glu Lys Val Leu Ser 65 70 75 gtt tta gat ttt tct acc cct ggt aga act gct gct gct gta gct ttc 289 Val Leu Asp Phe Ser Thr Pro Gly Arg Thr Ala Ala Ala Val Ala Phe 80 85 90 95 ctt act ttt ata ttg gat aaa tgg atc cca caa acc cac ttc agc aag 337 Leu Thr Phe Ile Leu Asp Lys Trp Ile Pro Gln Thr His Phe Ser Lys 100 105 110 gga tac gtc ttg gat ttc ata gca gca gct ttg tgg aga aca tgg aag 385 Gly Tyr Val Leu Asp Phe Ile Ala Ala Ala Leu Trp Arg Thr Trp Lys 115 120 125 gcc cgc agg ctg agg ata atc tta gat tac tgg cca gtg cag cct ctg 433 Ala Arg Arg Leu Arg Ile Ile Leu Asp Tyr Trp Pro Val Gln Pro Leu 130 135 140 ggc gta gca gca atc ctg aga cac cca ccg gcc atg cca gcg gtt ttg 481 Gly Val Ala Ala Ile Leu Arg His Pro Pro Ala Met Pro Ala Val Leu 145 150 155 gag gag gag cag cag gag gac aac ccg aga gcc ggc ttg gac cct ccg 529 Glu Glu Glu Gln Gln Glu Asp Asn Pro Arg Ala Gly Leu Asp Pro Pro 160 165 170 175 gtg gag gag gcg gag gag tag 550 Val Glu Glu Ala Glu Glu 180 <210> 93 <211> 181 <212> PRT <213> Simian adenovirus 29 <400> 93 Met Glu Val Trp Ala Ile Leu Glu Asp Leu Arg Gln Thr Arg Gln Leu 1 5 10 15 Leu Glu Asn Ala Ser Asp Gly Val Ser Ser Leu Trp Arg Phe Trp Phe 20 25 30 Gly Gly Asp Leu Ala Arg Leu Val Phe Arg Val Lys Arg Glu Tyr Ser 35 40 45 Glu Glu Phe Glu Lys Leu Leu Glu Asp Ser Pro Gly Leu Phe Glu Ala 50 55 60 Leu Asn Leu Gly His Gln Ala His Phe Lys Glu Lys Val Leu Ser Val 65 70 75 80 Leu Asp Phe Ser Thr Pro Gly Arg Thr Ala Ala Ala Val Ala Phe Leu 85 90 95 Thr Phe Ile Leu Asp Lys Trp Ile Pro Gln Thr His Phe Ser Lys Gly 100 105 110 Tyr Val Leu Asp Phe Ile Ala Ala Ala Leu Trp Arg Thr Trp Lys Ala 115 120 125 Arg Arg Leu Arg Ile Ile Leu Asp Tyr Trp Pro Val Gln Pro Leu Gly 130 135 140 Val Ala Ala Ile Leu Arg His Pro Pro Ala Met Pro Ala Val Leu Glu 145 150 155 160 Glu Glu Gln Gln Glu Asp Asn Pro Arg Ala Gly Leu Asp Pro Pro Val 165 170 175 Glu Glu Ala Glu Glu 180 <210> 94 <211> 1450 <212> DNA <213> Simian adenovirus 29 <220> <221> CDS <222> (574)..(1150) <223> label=Ela <220> <221> CDS <222> (1239)..(1444) <223> label=Ela <400> 94 catcatcaat aatatacctt ataaatggaa cggtgccaac atgcaaatga gcttttgaaa 60 atggagggcg gaaggggatt ggccagcggg ttcaacggtc aaaaggggcg ggccggcgcg 120 gggaggtgac gtatttcgtg tgggaggagt tatgttgcaa gttatcgcgg caaaagtgac 180 gtaaaacgag gtgtggtttg aacacggaag tagacagttt tcccgcgctg actgacagga 240 tatgaggtag ttttgggcgg atgcaagtga aaattctcca ttttcgcgcg aaaactgaat 300 gaggaagtga atttctgagt aatttcgagt ttatgacagg gtggagtatt taccgagggc 360 cgagtagact ttgaccgatt acgtggaggt ttcgattacc gtgtttttca cctaaatttc 420 cgcgtacggt gtcaaagtcc tgtgttttta cgtaggcgtc agctgatcgc tagggtattt 480 aaacctgacg agttccgtca agaggccact cttgagtgcc agcgagaaga gatttctcct 540 ccgcgccgcg agtcagatct ccactttgaa aaa atg aga cac ctg cga ttc ctg 594 Met Arg His Leu Arg Phe Leu 1 5 cct cag gaa atc tcc atc gag acc ggg aat gaa ata cta cag ctt gtg 642 Pro Gln Glu Ile Ser Ile Glu Thr Gly Asn Glu Ile Leu Gln Leu Val 10 15 20 gta aat gcc ctg atg gga gac gat ccg gag ccg cct gcg cat ccg ttc 690 Val Asn Ala Leu Met Gly Asp Asp Pro Glu Pro Pro Ala His Pro Phe 25 30 35 gat cct cct acg ctt cat gaa ctg tat gat tta gag gta gat ggg ccg 738 Asp Pro Pro Thr Leu His Glu Leu Tyr Asp Leu Glu Val Asp Gly Pro 40 45 50 55 gat gat cct aac gag gaa gct gtg aat ggt ttt ttt agc gaa tct atg 786 Asp Asp Pro Asn Glu Glu Ala Val Asn Gly Phe Phe Ser Glu Ser Met 60 65 70 cta ttg gct gct aat gaa gga gtg gac ata gac cca cct tct gag acc 834 Leu Leu Ala Ala Asn Glu Gly Val Asp Ile Asp Pro Pro Ser Glu Thr 75 80 85 ctc gat acc cca ggg gtg att gtg gag agc ggc aga ggt ggg aaa aaa 882 Leu Asp Thr Pro Gly Val Ile Val Glu Ser Gly Arg Gly Gly Lys Lys 90 95 100 ttg cct gaa ctt ggt gct gct gaa atg gac ttg cac tgt tat gaa gag 930 Leu Pro Glu Leu Gly Ala Ala Glu Met Asp Leu His Cys Tyr Glu Glu 105 110 115 ggt ttt cct ccg agt gat gat gaa gag gag gaa aat gtg cag tcg atc 978 Gly Phe Pro Pro Ser Asp Asp Glu Glu Glu Glu Asn Val Gln Ser Ile 120 125 130 135 cag acc gca gcg ggt gag gga atg aaa gct gcc aat gat ggt ttt aag 1026 Gln Thr Ala Ala Gly Glu Gly Met Lys Ala Ala Asn Asp Gly Phe Lys 140 145 150 ttg gac tgc ccg gag ctg cct gga cat ggc tgt aag tct tgt gaa ttt 1074 Leu Asp Cys Pro Glu Leu Pro Gly His Gly Cys Lys Ser Cys Glu Phe 155 160 165 cac agg aat agt act gga cta aaa gaa ctg ttg tgc tcg ctt tgc tat 1122 His Arg Asn Ser Thr Gly Leu Lys Glu Leu Leu Cys Ser Leu Cys Tyr 170 175 180 atg aga acg cac tgc cat ttt att tac a gtaagtgtgt ctaacttaaa 1170 Met Arg Thr His Cys His Phe Ile Tyr 185 190 tttaaaggga cagtgtagca gtttaatgtc tgttgaatgt gggatttatg tttttgtgat 1230 ttttatag gt cct gtg tct gat gct gat gaa tcg cct tct cct gat tca 1279 Ser Pro Val Ser Asp Ala Asp Glu Ser Pro Ser Pro Asp Ser 195 200 205 act acc tca cct cct gaa att cag gcg cca gtc cct gca aac gta tgc 1327 Thr Thr Ser Pro Pro Glu Ile Gln Ala Pro Val Pro Ala Asn Val Cys 210 215 220 aag ccc att cct gtg aag gct aag cct ggg aaa cgc cct gct gtg gat 1375 Lys Pro Ile Pro Val Lys Ala Lys Pro Gly Lys Arg Pro Ala Val Asp 225 230 235 aaa ctg gag gac ttg ctt gag ggt ggg gat gga cct ttg gac ttg agt 1423 Lys Leu Glu Asp Leu Leu Glu Gly Gly Asp Gly Pro Leu Asp Leu Ser 240 245 250 acc cgg aaa ctg cca agg caa tgagtg 1450 Thr Arg Lys Leu Pro Arg Gln 255 260 <210> 95 <211> 261 <212> PRT <213> Simian adenovirus 29 <400> 95 Met Arg His Leu Arg Phe Leu Pro Gln Glu Ile Ser Ile Glu Thr Gly 1 5 10 15 Asn Glu Ile Leu Gln Leu Val Val Asn Ala Leu Met Gly Asp Asp Pro 20 25 30 Glu Pro Pro Ala His Pro Phe Asp Pro Pro Thr Leu His Glu Leu Tyr 35 40 45 Asp Leu Glu Val Asp Gly Pro Asp Asp Pro Asn Glu Glu Ala Val Asn 50 55 60 Gly Phe Phe Ser Glu Ser Met Leu Leu Ala Ala Asn Glu Gly Val Asp 65 70 75 80 Ile Asp Pro Pro Ser Glu Thr Leu Asp Thr Pro Gly Val Ile Val Glu 85 90 95 Ser Gly Arg Gly Gly Lys Lys Leu Pro Glu Leu Gly Ala Ala Glu Met 100 105 110 Asp Leu His Cys Tyr Glu Glu Gly Phe Pro Pro Ser Asp Asp Glu Glu 115 120 125 Glu Glu Asn Val Gln Ser Ile Gln Thr Ala Ala Gly Glu Gly Met Lys 130 135 140 Ala Ala Asn Asp Gly Phe Lys Leu Asp Cys Pro Glu Leu Pro Gly His 145 150 155 160 Gly Cys Lys Ser Cys Glu Phe His Arg Asn Ser Thr Gly Leu Lys Glu 165 170 175 Leu Leu Cys Ser Leu Cys Tyr Met Arg Thr His Cys His Phe Ile Tyr 180 185 190 Ser Pro Val Ser Asp Ala Asp Glu Ser Pro Ser Pro Asp Ser Thr Thr 195 200 205 Ser Pro Pro Glu Ile Gln Ala Pro Val Pro Ala Asn Val Cys Lys Pro 210 215 220 Ile Pro Val Lys Ala Lys Pro Gly Lys Arg Pro Ala Val Asp Lys Leu 225 230 235 240 Glu Asp Leu Leu Glu Gly Gly Asp Gly Pro Leu Asp Leu Ser Thr Arg 245 250 255 Lys Leu Pro Arg Gln 260 <210> 96 <211> 900 <212> DNA <213> Simian adenovirus 29 <220> <221> CDS <222> (11)..(368) <223> label=33K <220> <221> CDS <222> (538)..(896) <223> label=33K <400> 96 ttccctcagg atg tcc cag cgc cga gga agc aag aag ttg aaa gtg cag 49 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln 1 5 10 ctg ccg ccc cca gag gac atg gag gaa gac tgg gac agt cag gca gag 97 Leu Pro Pro Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu 15 20 25 gag gag gag atg gaa gat tgg gac agc cag gca gag gag gtg gac agc 145 Glu Glu Glu Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Val Asp Ser 30 35 40 45 ctg gag gaa gac agt ttg gag gag gaa gac gag gag gca gag gag gtg 193 Leu Glu Glu Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val 50 55 60 gaa gaa gca gcc gcc gcc aaa cag ttg tcc tcg gca gcg gag aca agc 241 Glu Glu Ala Ala Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser 65 70 75 aag gtc cca gac agc agc agc agc acg gct aca atc tcc gct ccg ggt 289 Lys Val Pro Asp Ser Ser Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly 80 85 90 cgg ggg gcc cag cgg cgt ccc aac agt aga tgg gac gag acc ggg cga 337 Arg Gly Ala Gln Arg Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg 95 100 105 ttc ccg aac ccg acc acc gct tcc aag acc g gtaagaagga gcggcaggga 388 Phe Pro Asn Pro Thr Thr Ala Ser Lys Thr 110 115 tacaagtcct ggcgggggca taagaatgcc atcatctcct gcttgcatga atgcgggggc 448 aacatatcct tcacccggcg ctacctgctc ttccaccacg gggtgaactt cccccgcaat 508 gtcttgcatt actaccgtca cctccacag cc cct act aca gcc agc aag tcc 560 Ala Pro Thr Thr Ala Ser Lys Ser 125 cgg cag cct cgg cag aga aag aca gca gca gca gcg ggg acc tcc agc 608 Arg Gln Pro Arg Gln Arg Lys Thr Ala Ala Ala Ala Gly Thr Ser Ser 130 135 140 aga aaa cca gca gca gca gtt aga aaa tcc agt gca gca gga gga gga 656 Arg Lys Pro Ala Ala Ala Val Arg Lys Ser Ser Ala Ala Gly Gly Gly 145 150 155 ctg agg atc aca gcg aac gag cca gcg cag acc cga gag ctg aga aac 704 Leu Arg Ile Thr Ala Asn Glu Pro Ala Gln Thr Arg Glu Leu Arg Asn 160 165 170 175 agg atc ttt cca acc ctc tat gcc atc ttc cag cag agt cgg ggg caa 752 Arg Ile Phe Pro Thr Leu Tyr Ala Ile Phe Gln Gln Ser Arg Gly Gln 180 185 190 gag cag gaa ctg aaa gta aaa aac cga tct ctg cgc tcg ctc acc cga 800 Glu Gln Glu Leu Lys Val Lys Asn Arg Ser Leu Arg Ser Leu Thr Arg 195 200 205 agt tgt ttg tat cac aag agc gaa gac caa ctt cag cgc act ctc gag 848 Ser Cys Leu Tyr His Lys Ser Glu Asp Gln Leu Gln Arg Thr Leu Glu 210 215 220 gac gcc gag gct ctc ttc aac aag tac tgc gcg ctg act ctt aaa gag 896 Asp Ala Glu Ala Leu Phe Asn Lys Tyr Cys Ala Leu Thr Leu Lys Glu 225 230 235 tagc 900 <210> 97 <211> 239 <212> PRT <213> Simian adenovirus 29 <400> 97 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu Glu Glu Glu 20 25 30 Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Val Asp Ser Leu Glu Glu 35 40 45 Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu Ala 50 55 60 Ala Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Val Pro 65 70 75 80 Asp Ser Ser Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Arg Gly Ala 85 90 95 Gln Arg Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe Pro Asn 100 105 110 Pro Thr Thr Ala Ser Lys Thr Ala Pro Thr Thr Ala Ser Lys Ser Arg 115 120 125 Gln Pro Arg Gln Arg Lys Thr Ala Ala Ala Ala Gly Thr Ser Ser Arg 130 135 140 Lys Pro Ala Ala Ala Val Arg Lys Ser Ser Ala Ala Gly Gly Gly Leu 145 150 155 160 Arg Ile Thr Ala Asn Glu Pro Ala Gln Thr Arg Glu Leu Arg Asn Arg 165 170 175 Ile Phe Pro Thr Leu Tyr Ala Ile Phe Gln Gln Ser Arg Gly Gln Glu 180 185 190 Gln Glu Leu Lys Val Lys Asn Arg Ser Leu Arg Ser Leu Thr Arg Ser 195 200 205 Cys Leu Tyr His Lys Ser Glu Asp Gln Leu Gln Arg Thr Leu Glu Asp 210 215 220 Ala Glu Ala Leu Phe Asn Lys Tyr Cys Ala Leu Thr Leu Lys Glu 225 230 235 <210> 98 <211> 5093 <212> DNA <213> Simian adenovirus 29 <220> <221> CDS <222> (1)..(618) <223> label=22K <220> <221> CDS <222> (1642)..(1956) <223> label=E3\12.5K <220> <221> CDS <222> (2344)..(2877) <223> label=E3\gp19K <220> <221> CDS <222> (4674)..(5093) <223> label=E3\RID-beta <400> 98 atg tcc cag cgc cga gga agc aag aag ttg aaa gtg cag ctg ccg ccc 48 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 cca gag gac atg gag gaa gac tgg gac agt cag gca gag gag gag gag 96 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu Glu Glu Glu 20 25 30 atg gaa gat tgg gac agc cag gca gag gag gtg gac agc ctg gag gaa 144 Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Val Asp Ser Leu Glu Glu 35 40 45 gac agt ttg gag gag gaa gac gag gag gca gag gag gtg gaa gaa gca 192 Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu Ala 50 55 60 gcc gcc gcc aaa cag ttg tcc tcg gca gcg gag aca agc aag gtc cca 240 Ala Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Val Pro 65 70 75 80 gac agc agc agc agc acg gct aca atc tcc gct ccg ggt cgg ggg gcc 288 Asp Ser Ser Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Arg Gly Ala 85 90 95 cag cgg cgt ccc aac agt aga tgg gac gag acc ggg cga ttc ccg aac 336 Gln Arg Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe Pro Asn 100 105 110 ccg acc acc gct tcc aag acc ggt aag aag gag cgg cag gga tac aag 384 Pro Thr Thr Ala Ser Lys Thr Gly Lys Lys Glu Arg Gln Gly Tyr Lys 115 120 125 tcc tgg cgg ggg cat aag aat gcc atc atc tcc tgc ttg cat gaa tgc 432 Ser Trp Arg Gly His Lys Asn Ala Ile Ile Ser Cys Leu His Glu Cys 130 135 140 ggg ggc aac ata tcc ttc acc cgg cgc tac ctg ctc ttc cac cac ggg 480 Gly Gly Asn Ile Ser Phe Thr Arg Arg Tyr Leu Leu Phe His His Gly 145 150 155 160 gtg aac ttc ccc cgc aat gtc ttg cat tac tac cgt cac ctc cac agc 528 Val Asn Phe Pro Arg Asn Val Leu His Tyr Tyr Arg His Leu His Ser 165 170 175 ccc tac tac agc cag caa gtc ccg gca gcc tcg gca gag aaa gac agc 576 Pro Tyr Tyr Ser Gln Gln Val Pro Ala Ala Ser Ala Glu Lys Asp Ser 180 185 190 agc agc agc ggg gac ctc cag cag aaa acc agc agc agc agt 618 Ser Ser Ser Gly Asp Leu Gln Gln Lys Thr Ser Ser Ser Ser 195 200 205 tagaaaatcc agtgcagcag gaggaggact gaggatcaca gcgaacgagc cagcgcagac 678 ccgagagctg agaaacagga tctttccaac cctctatgcc atcttccagc agagtcgggg 738 gcaagagcag gaactgaaag taaaaaaccg atctctgcgc tcgctcaccc gaagttgttt 798 gtatcacaag agcgaagacc aacttcagcg cactctcgag gacgccgagg ctctcttcaa 858 caagtactgc gcgctgactc ttaaagagta gcccgcgccc gcgctcgctc gaaaaaggcg 918 ggaattacgt cacccttggc acctgtcctt tgccctcgtc atgagtaaag aaattcccac 978 gccttacatg tggagctatc agccccaaat gggactggca gccggcgcct cccaggacta 1038 ctccacccgc atgaattggc tcagcgccgg cccctcgatg atctcacggg ttaatgatat 1098 acgagcttac cgaaaccagt tactcctaga acagtcagct ctcaccacca caccccgcca 1158 acaccttaat ccccggaatt ggcccgccgc cctggtgtac caggaaactc ccgctcccac 1218 caccgtacta cttcctcgag acgcccaggc cgaagttcag atgactaacg caggtgtaca 1278 gctggcgggc ggttccgccc tgtgtcgtca ccggcctcag cagagtataa aacgcctggt 1338 gatcagaggc cgaggtatcc agctcaacga cgagtcggtg agctcttcgc ttggtctgcg 1398 accagacgga gtcttccaga tcgccggctg tgggagatct tccttcactc ctcgtcaggc 1458 tgtcctgact ttggagagtt cgtcctcgca gccccgctcg ggcggcatcg ggactctcca 1518 gtttgtggag gagtttactc cctctgtcta cttcaacccc ttctccggct ctcctggcca 1578 gtacccggac gagttcatac cgaacttcga cgcaatcagc gagtcagtgg atggctatga 1638 ttg atg tct ggt ggc gcg gct gag tta gct cga ctg cga cat cta gac 1686 Met Ser Gly Gly Ala Ala Glu Leu Ala Arg Leu Arg His Leu Asp 210 215 220 cac tgc cgc cgc ttt cgc tgt ttc gcc cgg gaa ctc acc gag ttc atc 1734 His Cys Arg Arg Phe Arg Cys Phe Ala Arg Glu Leu Thr Glu Phe Ile 225 230 235 tac ttc gaa ctc ccc gag gag cac cct cag gga ccg gcc cac gga gtg 1782 Tyr Phe Glu Leu Pro Glu Glu His Pro Gln Gly Pro Ala His Gly Val 240 245 250 cgg att acc atc gaa ggg ggg ata gac tct cac ctg cat cgg atc ttc 1830 Arg Ile Thr Ile Glu Gly Gly Ile Asp Ser His Leu His Arg Ile Phe 255 260 265 tgc cag cga ccc gtg ctg atc gag cgc gac cag gga act aca aca gtc 1878 Cys Gln Arg Pro Val Leu Ile Glu Arg Asp Gln Gly Thr Thr Thr Val 270 275 280 285 tcc atc tac tgc atc tgt aac cac ccc gga ttg cat gaa agc ctt tgc 1926 Ser Ile Tyr Cys Ile Cys Asn His Pro Gly Leu His Glu Ser Leu Cys 290 295 300 tgt ctt att tgt gct gag ttt aat aaa aac tgagttaaga ctcaccttcg 1976 Cys Leu Ile Cys Ala Glu Phe Asn Lys Asn 305 310 gactaccgct tcttcaaccc ggactttaca acaccagcca gaccctccgt tccagccaga 2036 agaaccagac ccttcctctg atccaggact ctaattctac ctccccagcg cctttcccta 2096 ctaaccttcc cgttactaac aacctcggag ctcagctgca tcaccgcttc tccagaagcc 2156 tcctttctgc caatattact actcccagaa ccggaggtga gctccgtggt ctccctactg 2216 acaacccctg ggtggtagcg ggttttgtag cgctaggagt agttgcgggt gggttggtgc 2276 ttatactctg ctacctatac acaccttgct gtgcttattt agtagtgttg tgttgctggt 2336 ttaagaa atg ggg gtc gta cta gta tcg ctt gct tta ctt tcg ctt ttg 2385 Met Gly Val Val Leu Val Ser Leu Ala Leu Leu Ser Leu Leu 315 320 325 ggt ctg ggc tct gct acg cta aga aat cag cct ttg cta tta gat ccc 2433 Gly Leu Gly Ser Ala Thr Leu Arg Asn Gln Pro Leu Leu Leu Asp Pro 330 335 340 gat gat gtt gat cca tgt ctg gac ttt gat cca gag aac tgc aca ctc 2481 Asp Asp Val Asp Pro Cys Leu Asp Phe Asp Pro Glu Asn Cys Thr Leu 345 350 355 act ttt gca cct gaa aca agt cgc ttc tgt gga gtt gtt att agg tgc 2529 Thr Phe Ala Pro Glu Thr Ser Arg Phe Cys Gly Val Val Ile Arg Cys 360 365 370 gga ttt gaa tgc agg tcc att gag att aca cac aat aac aaa act tgg 2577 Gly Phe Glu Cys Arg Ser Ile Glu Ile Thr His Asn Asn Lys Thr Trp 375 380 385 aac aat acc tta ttc aca ata tgg caa cca gga gat cct cag tgg tat 2625 Asn Asn Thr Leu Phe Thr Ile Trp Gln Pro Gly Asp Pro Gln Trp Tyr 390 395 400 405 act gtc tct gtc cgg ggt cct gac ggt tcc atc cgc atg gct aat aac 2673 Thr Val Ser Val Arg Gly Pro Asp Gly Ser Ile Arg Met Ala Asn Asn 410 415 420 act ttc att ttt gct gaa atg tgc gat atg gcc atg ttc atg agc aga 2721 Thr Phe Ile Phe Ala Glu Met Cys Asp Met Ala Met Phe Met Ser Arg 425 430 435 cag tat gac cta tgg cct ccc agc aaa gag aac att gtg gct ttc tcc 2769 Gln Tyr Asp Leu Trp Pro Pro Ser Lys Glu Asn Ile Val Ala Phe Ser 440 445 450 att gct tat tgc ttg tgt act tgc atc atc act gct atc atg tgt gtg 2817 Ile Ala Tyr Cys Leu Cys Thr Cys Ile Ile Thr Ala Ile Met Cys Val 455 460 465 agc ata cac ttg ctt ata gcc att cgc cca aaa aac aat caa gaa aaa 2865 Ser Ile His Leu Leu Ile Ala Ile Arg Pro Lys Asn Asn Gln Glu Lys 470 475 480 485 gag aaa atg ccc tgattataaa tttctattta cagaaaatga cctctgtttc 2917 Glu Lys Met Pro agctctcata tttgctacta ttatggctgt tcaaggacag gctgctcaag gacagacact 2977 tattaatgtt catcctggaa ctaatcatac cttggtggtt cctaataact attcaaatat 3037 tgaatggcaa tggttcacaa acaacgtatg gtatgaacca tgcgaacatt acagcctatt 3097 catttgcaat cataatttaa ctttaatcaa tgtcagcaca atacacaaag gatactatta 3157 tagatatgac aaccacagca ttgatcctac aatatatcta gtacgtgtaa atccaattaa 3217 caaacctata cccaaagctt tctctagaac tacaatacaa aactttaaaa cagcaatttt 3277 acttaatttt aaaaccaaaa atattacagg caatatactt cccactactc ccactgaaaa 3337 aaatacacct aattcaatat ttgaaatcat cattgcactg ttagcagtag gcataacaat 3397 catactatgt atgataattt atgctcactg ttataaaaaa attcaccaca aaaaagaacc 3457 actactaagc ttttaatttc ttttttatac agccatgatt ttcttcgcaa ctcttattac 3517 tattggcatt gttcaagggc aagatatcac aattggatat gtaggcaata atattaccct 3577 attaggtccc ccaacaggaa caatccctac ctggtacaaa atatatgaaa gagggtggtg 3637 gattagaccc tgcgaccaag gaggtagtaa atacatttgt ggtagagaca taaccatcac 3697 caatcttaat aaaaacgata atggctacta tttttgcaat aactatggag gtggtaaaaa 3757 gtcttacaca cttgaagtaa gagaccccac cactttagca ccacatacca ctttctccag 3817 cagcacgtct agaaacacac atgaggcagc ttatgccaga gcaatgcttc aaaaaattaa 3877 tgaaacaata aattctacaa tctctcataa tccagacgaa attcccaaat caatgattgg 3937 cattattgta gccgtggcag ttggaatggc aatcataata atttgtatga tcgtctatgc 3997 ttgctgctat agaaagtttc aagatgaaaa aggagaccca ctactaagct ttgatattta 4057 atttctttat agaaacatga aaggagtagg tatcctagtt ctttcaactt taatctactc 4117 agtgatccct atcagcatca atgtgcagac tactttaaat gaaactggaa accactcaac 4177 tacctcacat acacctcccc cgctttctac ccaccctcaa tccaaagatg ccatacaact 4237 acaactcacc atccttattg tgattgggtt aactatcctt gctgttatcc tttactttat 4297 cttttgccgc caaataccca atgtagttaa gaaacctacc agacgtccca tctatcgatc 4357 aataatcagc aaaccccaca tggctctaaa tgaaatttaa tctttctctt cacagtatgg 4417 tgatcaacta tgatccctag aaatttcttc ttcaccatac ttatctgcgc tttcaatgtc 4477 tgtgctacat tcgccacagt cgccaatgtg acaccagatt gtataggggc atttgcttcc 4537 tacgtactat ttgccttcat tacctgcatc tgcgtttgta gcatagtctg cctggttatc 4597 aacttctttc aactagtaga ctgggttttt gtacgcattg cctacctacg acatcaccct 4657 gaataccgca accaaa atg ttg cag caa ttc tta ggc tca ttt aaa acc atg 4709 Met Leu Gln Gln Phe Leu Gly Ser Phe Lys Thr Met 490 495 500 caa act ctg cta ctg ctt ctg cta gtt ata cac caa tgt gcc tca aac 4757 Gln Thr Leu Leu Leu Leu Leu Leu Val Ile His Gln Cys Ala Ser Asn 505 510 515 ccc aca agc ccc aca aga tta gat cta aga aaa tgt aaa ttt caa gaa 4805 Pro Thr Ser Pro Thr Arg Leu Asp Leu Arg Lys Cys Lys Phe Gln Glu 520 525 530 cca tgg aaa ttc ctt gat tgc tat cat gaa aca tct gat ttc ccc aca 4853 Pro Trp Lys Phe Leu Asp Cys Tyr His Glu Thr Ser Asp Phe Pro Thr 535 540 545 tac tgg att aca atc att ggg gtt gtt aat cta gtc tct tgc aca cta 4901 Tyr Trp Ile Thr Ile Ile Gly Val Val Asn Leu Val Ser Cys Thr Leu 550 555 560 565 ttc tct ttc ctt gtt tac cac tta ttt gat ttt gga tgg aac gcc ctt 4949 Phe Ser Phe Leu Val Tyr His Leu Phe Asp Phe Gly Trp Asn Ala Leu 570 575 580 aat gca ctc act tac cca caa gaa cca gag gaa cat ata cca cta cag 4997 Asn Ala Leu Thr Tyr Pro Gln Glu Pro Glu Glu His Ile Pro Leu Gln 585 590 595 aac ata caa cca tta gca cta gta gaa tat gaa aat gag cca cag cct 5045 Asn Ile Gln Pro Leu Ala Leu Val Glu Tyr Glu Asn Glu Pro Gln Pro 600 605 610 cca cta ctc cct gcc att agc tac ttc aac cta acc ggt gga gat gac 5093 Pro Leu Leu Pro Ala Ile Ser Tyr Phe Asn Leu Thr Gly Gly Asp Asp 615 620 625 <210> 99 <211> 206 <212> PRT <213> Simian adenovirus 29 <400> 99 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu Glu Glu Glu 20 25 30 Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Val Asp Ser Leu Glu Glu 35 40 45 Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu Ala 50 55 60 Ala Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Val Pro 65 70 75 80 Asp Ser Ser Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Arg Gly Ala 85 90 95 Gln Arg Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe Pro Asn 100 105 110 Pro Thr Thr Ala Ser Lys Thr Gly Lys Lys Glu Arg Gln Gly Tyr Lys 115 120 125 Ser Trp Arg Gly His Lys Asn Ala Ile Ile Ser Cys Leu His Glu Cys 130 135 140 Gly Gly Asn Ile Ser Phe Thr Arg Arg Tyr Leu Leu Phe His His Gly 145 150 155 160 Val Asn Phe Pro Arg Asn Val Leu His Tyr Tyr Arg His Leu His Ser 165 170 175 Pro Tyr Tyr Ser Gln Gln Val Pro Ala Ala Ser Ala Glu Lys Asp Ser 180 185 190 Ser Ser Ser Gly Asp Leu Gln Gln Lys Thr Ser Ser Ser Ser 195 200 205 <210> 100 <211> 105 <212> PRT <213> Simian adenovirus 29 <400> 100 Met Ser Gly Gly Ala Ala Glu Leu Ala Arg Leu Arg His Leu Asp His 1 5 10 15 Cys Arg Arg Phe Arg Cys Phe Ala Arg Glu Leu Thr Glu Phe Ile Tyr 20 25 30 Phe Glu Leu Pro Glu Glu His Pro Gln Gly Pro Ala His Gly Val Arg 35 40 45 Ile Thr Ile Glu Gly Gly Ile Asp Ser His Leu His Arg Ile Phe Cys 50 55 60 Gln Arg Pro Val Leu Ile Glu Arg Asp Gln Gly Thr Thr Thr Val Ser 65 70 75 80 Ile Tyr Cys Ile Cys Asn His Pro Gly Leu His Glu Ser Leu Cys Cys 85 90 95 Leu Ile Cys Ala Glu Phe Asn Lys Asn 100 105 <210> 101 <211> 178 <212> PRT <213> Simian adenovirus 29 <400> 101 Met Gly Val Val Leu Val Ser Leu Ala Leu Leu Ser Leu Leu Gly Leu 1 5 10 15 Gly Ser Ala Thr Leu Arg Asn Gln Pro Leu Leu Leu Asp Pro Asp Asp 20 25 30 Val Asp Pro Cys Leu Asp Phe Asp Pro Glu Asn Cys Thr Leu Thr Phe 35 40 45 Ala Pro Glu Thr Ser Arg Phe Cys Gly Val Val Ile Arg Cys Gly Phe 50 55 60 Glu Cys Arg Ser Ile Glu Ile Thr His Asn Asn Lys Thr Trp Asn Asn 65 70 75 80 Thr Leu Phe Thr Ile Trp Gln Pro Gly Asp Pro Gln Trp Tyr Thr Val 85 90 95 Ser Val Arg Gly Pro Asp Gly Ser Ile Arg Met Ala Asn Asn Thr Phe 100 105 110 Ile Phe Ala Glu Met Cys Asp Met Ala Met Phe Met Ser Arg Gln Tyr 115 120 125 Asp Leu Trp Pro Pro Ser Lys Glu Asn Ile Val Ala Phe Ser Ile Ala 130 135 140 Tyr Cys Leu Cys Thr Cys Ile Ile Thr Ala Ile Met Cys Val Ser Ile 145 150 155 160 His Leu Leu Ile Ala Ile Arg Pro Lys Asn Asn Gln Glu Lys Glu Lys 165 170 175 Met Pro <210> 102 <211> 140 <212> PRT <213> Simian adenovirus 29 <400> 102 Met Leu Gln Gln Phe Leu Gly Ser Phe Lys Thr Met Gln Thr Leu Leu 1 5 10 15 Leu Leu Leu Leu Val Ile His Gln Cys Ala Ser Asn Pro Thr Ser Pro 20 25 30 Thr Arg Leu Asp Leu Arg Lys Cys Lys Phe Gln Glu Pro Trp Lys Phe 35 40 45 Leu Asp Cys Tyr His Glu Thr Ser Asp Phe Pro Thr Tyr Trp Ile Thr 50 55 60 Ile Ile Gly Val Val Asn Leu Val Ser Cys Thr Leu Phe Ser Phe Leu 65 70 75 80 Val Tyr His Leu Phe Asp Phe Gly Trp Asn Ala Leu Asn Ala Leu Thr 85 90 95 Tyr Pro Gln Glu Pro Glu Glu His Ile Pro Leu Gln Asn Ile Gln Pro 100 105 110 Leu Ala Leu Val Glu Tyr Glu Asn Glu Pro Gln Pro Pro Leu Leu Pro 115 120 125 Ala Ile Ser Tyr Phe Asn Leu Thr Gly Gly Asp Asp 130 135 140 <210> 103 <211> 35588 <212> DNA <213> Simian adenovirus 32 <220> <221> repeat_region <222> (1)..(131) <223> label=ITR <220> <221> CDS <222> (1919)..(3403) <223> label=Elb\55K <220> <221> CDS <222> (3500)..(3913) <223> label=pIX <220> <221> misc_feature <222> (3982)..(5603) <223> complement (3982..5312, 5591..5603) label=IVa2 <220> <221> misc_feature <222> (5085)..(13880) <223> complement (5085..8654, 13872..13880) label=pol <220> <221> misc_feature <222> (8456)..(13880) <223> complement (8456..10399, 13872..13880) label=pTP <220> <221> CDS <222> (10883)..(12049) <223> label=52K <220> <221> CDS <222> (12077)..(13837) <223> label=pIIIa <220> <221> CDS <222> (13916)..(15670) <223> label=penton <220> <221> CDS <222> (15683)..(16258) <223> label=pVII <220> <221> CDS <222> (16304)..(17353) <223> label=V <220> <221> CDS <222> (17385)..(17609) <223> label=pX <220> <221> CDS <222> (17687)..(18436) <223> label=pVI <220> <221> CDS <222> (18555)..(21419) <223> label=hexon <220> <221> CDS <222> (21455)..(22081) <223> label=protease <220> <221> misc_feature <222> (22176)..(23729) <223> complement label=DBP <220> <221> CDS <222> (23760)..(26255) <223> label=100K <220> <221> CDS <222> (26905)..(27585) <223> label=pVIII <220> <221> CDS <222> (27588)..(27902) <223> label=E3\12.5K <220> <221> CDS <222> (28290)..(28823) <223> label=E3\gp19K <220> <221> CDS <222> (28854)..(29465) <223> label=E3\CR1\beta <220> <221> CDS <222> (29487)..(30293) <223> label=E3\CR1\gamma <220> <221> CDS <222> (30306)..(30578) <223> label=E3\RID\alpha <220> <221> CDS <222> (30971)..(31372) <223> label=E3\14.7K <220> <221> CDS <222> (31609)..(32565) <223> label=fiber <220> <221> misc_feature <222> (32614)..(33773) <223> complement (32614..32862, 33576..33773) label=E4\orf6/7 <220> <221> misc_feature <222> (32862)..(33773) <223> complement label=E4\orf6 <220> <221> misc_feature <222> (33664)..(34044) <223> complement label=E4\orf4 <220> <221> misc_feature <222> (34057)..(34407) <223> complement label=E4\orf3 <220> <221> misc_feature <222> (34407)..(34793) <223> complement label=E4\orf2 <220> <221> misc_feature <222> (34837)..(35208) <223> complement label=E4\orf1 <220> <221> repeat_region <222> (35458)..(35588) <223> complement label=ITR <400> 103 catcatcaat aatatacctt ataaatggaa cggtgccaac atgcaaatga gcttttgaaa 60 atggagggcg gaaggggatt ggccagcggg ttcaacggtc aaaaggggcg ggccggcgcg 120 gggaggtgac gtgtttagtg tgggaggagt tatgttgcaa gttctcgcgg taaatgtgac 180 gtaaaacgag gtgtggtttg aacacggaag tggacagttt tcccgcgctg actgacagga 240 tatgaggtag ttttgggcgg atgcaagtga aaattctcca ttttcgcgcg aaaactgaat 300 gaggaagtga atttctgagt aatttcgagt ttatgacagg gcggagtatt taccgagggc 360 cgagtagact ttgaccgatt acgtggaggt ttcgattacc gtgtttttca cctaaatttc 420 cgcgtacggt gtcaaagtcc tgtgttttta cgtaggcgtc agctgatcgc tagggtattt 480 aaacctgacg agttccgtca agaggccact cttgagtgcc agcgagaaga gatttctcct 540 ccgcgccgcg agtcagatct ccactttgaa aaatgagaca cctgcgattc ctgcctcagg 600 aaatctccat cgagaccggg aatgaaatac tacagcttgt ggtaaatgcc ctgatgggag 660 acgatccgga gccgcctgcg catccgttcg atcctcctac gcttcatgaa ctgtatgatt 720 tagaggtaga cgggccggag gatcctaacg aggaagctgt gaatggtttt tttagcgaat 780 ctatgctatt ggctgctaat gaaggagtgg acatagaccc accgtcggag accctcgata 840 ccccaggggt gattgtggag agcggcagag gtgggaaaac attgcctgaa cttggtgctg 900 ctgaaatgga cttgcgctgt tatgaagagg gctttcctcc gagtgatgat gaagaggagg 960 aaaatgtgca gtcgatccag accgcagcgg gtgagggaat gaaagctgcc aatgatggtt 1020 ttaagttgga ctgcccggag ctgcctggac atggctgtaa gtcttgtgaa tttcacagga 1080 atagtactgg actaaaagaa ctgttgtgct cgctttgcta tatgagaacg cactgccatt 1140 ttatttacag taagtgtgtc taacttaaat ttaaagggac agtgtagcag tttagtgtct 1200 gttgaatgtg ggatttatgt ctttgtgatt tttataggtc ctgtgtctga tgctgatgaa 1260 tcgccttctc ctgattcaac tacctcacct cctgaaattc aggcgccagt ccctgcaaac 1320 gtatgcaagc ccattcctgt gaaggctaag cctgggaaac gccctgctgt ggataaactg 1380 gaggacttgc ttgagggtgg ggatggacct ttggacttga gtacccggaa actgccaagg 1440 caatgagtgc cctgcacctg tgtttattta atgtgacgtc agtatttatg tgagagtacc 1500 atgtaataaa attatgtcag ctgctgagta ttttattgct tcttgggtgg ggacttggat 1560 atataagtag gagcagacct gtgtggttag ctcacagcag cttgctgcca tccatggagg 1620 tttgggctat cttggaagat ctcaggcaga ctagacaact gctagaaaac gcctcggacg 1680 gagtctctag tctttggaga ttctggttcg gtggtgatct agctaggcta gtctttaggg 1740 taaaacggga gtatagtgaa gaatttgaaa agttattgga agacagtcca ggactttttg 1800 aagcccttaa cttgggccac caggctcatt ttaaggagaa ggttttatca gttttagatt 1860 tttctacccc tggtagaact gctgctgctg tagctttcct tacttttata ttggataa 1918 atg gat ccc aca aac cca ctt cag caa ggg ata cgt ctt gga ttt cat 1966 Met Asp Pro Thr Asn Pro Leu Gln Gln Gly Ile Arg Leu Gly Phe His 1 5 10 15 agc agc agc ttt gtg gag aac atg gaa ggc ccg cag gct gag gat aat 2014 Ser Ser Ser Phe Val Glu Asn Met Glu Gly Pro Gln Ala Glu Asp Asn 20 25 30 ctt aga tta ctg gcc agt gca gcc tct ggg cgt agc agc aat cct gag 2062 Leu Arg Leu Leu Ala Ser Ala Ala Ser Gly Arg Ser Ser Asn Pro Glu 35 40 45 aca ccc acc ggc cat gcc agc ggt ttt gga gga gga gca gca gga gga 2110 Thr Pro Thr Gly His Ala Ser Gly Phe Gly Gly Gly Ala Ala Gly Gly 50 55 60 caa ccc gag agc cgg cct gga ccc tcc ggt gga gga ggc gga gga gta 2158 Gln Pro Glu Ser Arg Pro Gly Pro Ser Gly Gly Gly Gly Gly Gly Val 65 70 75 80 gct gac ctg ttt cct gaa ctg cga cgg gtg ctt act agg tct acg tcc 2206 Ala Asp Leu Phe Pro Glu Leu Arg Arg Val Leu Thr Arg Ser Thr Ser 85 90 95 agt gga cag gac agg ggc att aag agg gag agg aat gct agt ggg cat 2254 Ser Gly Gln Asp Arg Gly Ile Lys Arg Glu Arg Asn Ala Ser Gly His 100 105 110 aat tca aga act gag ttg gct tta agt tta atg agt cgc agc cgc cct 2302 Asn Ser Arg Thr Glu Leu Ala Leu Ser Leu Met Ser Arg Ser Arg Pro 115 120 125 gaa act atc tgg tgg cat gag gtt cag agc gag ggc agg gat gaa gtt 2350 Glu Thr Ile Trp Trp His Glu Val Gln Ser Glu Gly Arg Asp Glu Val 130 135 140 tca ata ttg cag gaa aaa tat tct cta gaa caa att aaa acc tgt tgg 2398 Ser Ile Leu Gln Glu Lys Tyr Ser Leu Glu Gln Ile Lys Thr Cys Trp 145 150 155 160 ttg gaa cct gag gat gat tgg gag gtg gcc att agg aat tat gct aag 2446 Leu Glu Pro Glu Asp Asp Trp Glu Val Ala Ile Arg Asn Tyr Ala Lys 165 170 175 ata tct ctg agg cct gat aaa cag tat aaa att acc aaa aag att aat 2494 Ile Ser Leu Arg Pro Asp Lys Gln Tyr Lys Ile Thr Lys Lys Ile Asn 180 185 190 atc aga aat gca tgc tac ata gca ggg aat ggg gcc gag gtt ata ata 2542 Ile Arg Asn Ala Cys Tyr Ile Ala Gly Asn Gly Ala Glu Val Ile Ile 195 200 205 gat aca cca gat aaa aca gct ttt agg tgt tgc atg atg ggt atg tgg 2590 Asp Thr Pro Asp Lys Thr Ala Phe Arg Cys Cys Met Met Gly Met Trp 210 215 220 cca ggg gtg gct ggc atg gag gcg gtg acc ctt atg aat ata agg ttt 2638 Pro Gly Val Ala Gly Met Glu Ala Val Thr Leu Met Asn Ile Arg Phe 225 230 235 240 agg gga gat ggg tat aat ggg att gtc ttt atg gct aac act aag ctg 2686 Arg Gly Asp Gly Tyr Asn Gly Ile Val Phe Met Ala Asn Thr Lys Leu 245 250 255 att ctg cat ggt tgt agc ttt ttt ggg ttt aat aat act tgt gtg gaa 2734 Ile Leu His Gly Cys Ser Phe Phe Gly Phe Asn Asn Thr Cys Val Glu 260 265 270 tct tgg gga caa gtc agt atc agg ggt tgt agt ttc tat gca tgc tgg 2782 Ser Trp Gly Gln Val Ser Ile Arg Gly Cys Ser Phe Tyr Ala Cys Trp 275 280 285 att gca cta tca ggc aga acc aag agt cag ttg tct gtg aag aaa tgc 2830 Ile Ala Leu Ser Gly Arg Thr Lys Ser Gln Leu Ser Val Lys Lys Cys 290 295 300 atg ttc gag aga tgt aac ctg ggc ata ctg aat gaa ggt gaa gca agg 2878 Met Phe Glu Arg Cys Asn Leu Gly Ile Leu Asn Glu Gly Glu Ala Arg 305 310 315 320 gtc cgc cac tgt gct gct aca gaa act ggc tgc ttc att cta ata aag 2926 Val Arg His Cys Ala Ala Thr Glu Thr Gly Cys Phe Ile Leu Ile Lys 325 330 335 gga aat gcc agt gtg agg cat aac atg atc tgt gga ccc tcg gat gag 2974 Gly Asn Ala Ser Val Arg His Asn Met Ile Cys Gly Pro Ser Asp Glu 340 345 350 agg cct tat cag atg ctg acc tgt gct gga gga cat tgc aat atg ctg 3022 Arg Pro Tyr Gln Met Leu Thr Cys Ala Gly Gly His Cys Asn Met Leu 355 360 365 gct act gtg cat att gtt tct cat gca cgc aag aaa tgg cct gtt ttt 3070 Ala Thr Val His Ile Val Ser His Ala Arg Lys Lys Trp Pro Val Phe 370 375 380 gag cat aat gtg atg acc aag tgc acc atg cac ata ggt ggt cgc agg 3118 Glu His Asn Val Met Thr Lys Cys Thr Met His Ile Gly Gly Arg Arg 385 390 395 400 gga atg ttt atg cct tac cag tgt aac atg aat cat gtg aag gtg atg 3166 Gly Met Phe Met Pro Tyr Gln Cys Asn Met Asn His Val Lys Val Met 405 410 415 ttg gaa cca gat gcc ttt tcc aga atg agc tta aca gga atc ttt gat 3214 Leu Glu Pro Asp Ala Phe Ser Arg Met Ser Leu Thr Gly Ile Phe Asp 420 425 430 atg aat gtg caa cta tgg aag atc ctg aga tat gat gag acc aaa tcg 3262 Met Asn Val Gln Leu Trp Lys Ile Leu Arg Tyr Asp Glu Thr Lys Ser 435 440 445 agg gta cgc gca tgc gaa tgc ggg ggc aag cat gcc agg ttc cag ccg 3310 Arg Val Arg Ala Cys Glu Cys Gly Gly Lys His Ala Arg Phe Gln Pro 450 455 460 gtg tgt gtg gat gtg acg gaa gac ctg aga ccc gat cat ttg gtg ctt 3358 Val Cys Val Asp Val Thr Glu Asp Leu Arg Pro Asp His Leu Val Leu 465 470 475 480 gcc tgc act gga gcg gag ttc ggt tct agt ggg gaa gaa act gac 3403 Ala Cys Thr Gly Ala Glu Phe Gly Ser Ser Gly Glu Glu Thr Asp 485 490 495 taaagtgagt agtgtgggga tgctgtggag ggggcttcca ggcgggtaag gtgggcagat 3463 tgggtaaatt ctgtttgttt ctgtcttgca gctgtc atg agt gga agc gct tct 3517 Met Ser Gly Ser Ala Ser 500 ttt gag ggg gga gtc ttt agc cct tat ctg acg ggc agg ctc cca ccc 3565 Phe Glu Gly Gly Val Phe Ser Pro Tyr Leu Thr Gly Arg Leu Pro Pro 505 510 515 tgg gca gga gtt cgt cag aat gtc atg gga tcc act gtg gat ggg aga 3613 Trp Ala Gly Val Arg Gln Asn Val Met Gly Ser Thr Val Asp Gly Arg 520 525 530 ccc gtc cag ccc gcc aat tcc tca acg ctg acc tat gcc act ttg agc 3661 Pro Val Gln Pro Ala Asn Ser Ser Thr Leu Thr Tyr Ala Thr Leu Ser 535 540 545 tct tca ccc ttg gat gca gcc gca gcc gct gcc gcc tct gct gcc gcc 3709 Ser Ser Pro Leu Asp Ala Ala Ala Ala Ala Ala Ala Ser Ala Ala Ala 550 555 560 565 aac acc gtc ctt gga atg ggc tat tat gga agc atc gtt gcc aat tcc 3757 Asn Thr Val Leu Gly Met Gly Tyr Tyr Gly Ser Ile Val Ala Asn Ser 570 575 580 agt tcc tct aat aac cct tcg acc ctg gct gag gac aag cta ctt gtc 3805 Ser Ser Ser Asn Asn Pro Ser Thr Leu Ala Glu Asp Lys Leu Leu Val 585 590 595 ctc ttg gct cag ctc gag gcc ttg acc cag cgc cta ggc gaa ctg tct 3853 Leu Leu Ala Gln Leu Glu Ala Leu Thr Gln Arg Leu Gly Glu Leu Ser 600 605 610 cag cag gtg gcc cag ttg cgc gag caa act gag tct gct gtt gcc aca 3901 Gln Gln Val Ala Gln Leu Arg Glu Gln Thr Glu Ser Ala Val Ala Thr 615 620 625 gca aag tct aaa taaagattcc caaatcaata aataaaggag atccttgttg 3953 Ala Lys Ser Lys 630 attgtaaaac aagtgtaatg aatctttatt tgatttttcg cgcgcggtat gccctggacc 4013 accggtctcg atcattgaga actcggtgga tcttttccag gaccctgtag aggtgggatt 4073 gaatgtttag atacatgggc attaggccgt ctcgggggtg gagatagctc cattgaagag 4133 cctcatgctc cggggtagtg ttataaatca cccagtcata acaaggtcgg agtgcatggt 4193 gttgcacaat atcttttagg agcaggctaa ttgcaaccgg gaggccctta gtgtaggtgt 4253 ttacaaatct gttgagctgg gacgggtgca tccggggtga aattatatgc attttggact 4313 ggatcttgag gttggcaatg ttgccgccta gatcccgtct cgggttcata ttgtgcagga 4373 ccaccaagac agtgtatccg gtgcacttgg gaaatttatc atgcagctta gagggaaaag 4433 catgaaaaaa tttggagacg cctttgtgtc cgcccagatt ctccatgcac tcatccataa 4493 tgatagcgat gggaccgtgg gcggcggcgc gggcaaacac gttcccgggg tctgacacat 4553 catagttatg ctcctgagtc aggtcatcat aagccatttt aataaacttg gggcggaggg 4613 tgccagattg ggggatgaaa gttccctcgg gccccggagc atagtttccc tcacatattt 4673 gcatttccca agctttcagt tcagaggggg ggatcatgtc cacctgcggg gctataaaaa 4733 ataccgtttc tggagccggg gtgattaact gggatgagag cagattcctg agcagctgag 4793 acttgccgca cccagtggga ccgtaaatga ccccgattac gggttgcaga tggtagttta 4853 gggagcggca gctgccgtcc tcccggagca ggggggccac ttcgttcatc atttccctta 4913 catggatatt ttcccgcacc aagtccgtta ggaggcgctc tccccccagg gatagaagct 4973 cctggagcga ggagaagttt ttcagcggct tcagcccgtc agccatgggc attttggaga 5033 gagtctgttg caagagctct agtcggtccc agagctcggt gatgtgttct atggcatctc 5093 gatccagcag acctcctcgt ttcgcgggtt gggacgactc ctggagtatg gtatcagacg 5153 atgggcgtcc agcgctgcca gggtccgatc tttccagggt cgcagcgtcc gagtcagggt 5213 tgtttccgtc acggtgaagg ggtgcgcgcc tggttgggcg cttgcgaggg tgcgtttcag 5273 gctcatcctg ctggtcgaga accgctgccg atcggcgccc tgcatgtcgg ccaggtagca 5333 gtttaccatg agttcgtagt tgagcgcctc ggccgcgtgg cctttggcgc ggagcttacc 5393 tttggaagtt ttctggcagg cggggcagta cagacacttg agggcataca gtttgggagc 5453 gaggaagatg gattcggggg agtatgcgtc cgcaccgcag gaggcgcaga cggtttcgca 5513 ttccacgagc caggtcagat ccggctcatc ggggtcaaaa acaagttttc ccccatgttt 5573 tttgatgcgt ttcttacctt tggtctccat gagttcgtgt ccccgctggg tgacaaagag 5633 gctgtccgtg tccccgtaga ccgattttat gggcctgtcc tcgagcggag tgcctcggtc 5693 ctcttcgtag aggaactcgg accactctga tacaaaggcg cgcgtccagg ccagcacaaa 5753 agaggccacg tgggaggggt agcggtcgtt gtcaaccagg gggtccacct tctccacggt 5813 atgtaaacac atgtccccct cctccacatc caagaatgtg attggcttgt aagtgtatgc 5873 cacgtgacca ggggtccccg ccgggggggt ataaaagggg gcgggtctct gctcgtcctc 5933 actgtcttcc ggatcgctgt ccaggagcgc cagctgttgg ggtaggtatt ccctctcgaa 5993 ggcgggcata acctctgcac tcaggttgtc agtttctagg aacgaggagg atttgatatt 6053 gacagtgcca gccgagatgc ctttcataag actctcgtcc atttggtcag aaaatacaat 6113 ctttttgttg tccagcttgg tggcaaagga tccatagagg gcattggata agagcttggc 6173 tatggagcgc atggtttggt tcttttcctt gtcagcgcgc tccttggcag caatgttgag 6233 ctggacatac tcgcgcgcca gacacttcca ttcagggaag atggttgtca gttcatctgg 6293 cacgattctg actcgccagc ccctgttatg cagggtgatc agatccacac tggtggtcac 6353 ttcgcctctg aggggctcgt tggtccagca gagtcggccc ccttttctcg aacagaaagg 6413 tgggaggggg tctagcatga gttcatcagg ggggtctgca tccatagtga agattcctgg 6473 gagcagatcc ttgtcaaaat agctgatggg tgtggggtca tccaaagcca tctgccattc 6533 tcgagctgcc agcgcgcgct cataggggtt gagaggggtg ccccatggca tggggtgggt 6593 gagtgcagag gcatacatgc cacagatgtc atagacatag aggggctctt cgaggatgcc 6653 aatgtaggtg ggataacagc gcccccctct gatgcttgct cgcacatagt catagagttc 6713 atgcgagggg gcgagcagac ccgagcccag attagtgcga ttgggttttt cagccctgta 6773 gacgatctgg cgaaagatgg catgtgaatt tgaagagatg gtgggtctct gaaagatgtt 6833 aaaatgggca tgaggtagac ctacagagtc cctgatgaag tgggcatatg actcttgcag 6893 cttggccacc agctctgcag tgacaaggac atccaaggcg cagtagtcaa gggtctcttg 6953 gatgatgtca taacctggtt ggtttttctt ttcccacagc tcgcggttga gaaggtattc 7013 ttcgcgatcc ttccagtact cttcgagggg aaacccgtct ttgtctgcac ggtaagagcc 7073 cagcatgtag aactgattga ctgctttgta gggacagcag cccttctcca cggggagaga 7133 gtatgcttgg gctgccttgc gcagtgaggt atgagtgagg gcgaaggtgt ccctgaccat 7193 gactttgagg aactggtact tgaagtcaat gtcatcacag gccccctgtt cccagagttg 7253 gaagtctacc cgcttcttgt aggcggggtt gggcaaagcg aaagtaacat cattaaagag 7313 aatcttgccg gccctgggca tgaaattgcg ggtgatgcgg aaaggctggg gcacctctgc 7373 ccggttattg atcacctgag cggctaggac gatctcatca aagccattga tgttgtgccc 7433 cacaatgtaa agttctatga atcgcggggt gcccctgaca tgaggcagct tcttgagttc 7493 ttcaaaagtg aggtctgtag ggtcagagag agcatagtgt tcgagggccc attcgtgcag 7553 gtgagggttt gcattgagga aggaggacca gagatccact gccagtgctg tttgtaactg 7613 gtcccgatac tggcgaaaat gctggccgac tgccatcttt tctggggtta tacagtagaa 7673 ggttttgggg tcttgctgcc agcgatccca cttgagtttc atggcgaggt cgtaggcgat 7733 attgacgagc cgctcgtccc ccgagagttt catgaccagc atgaagggga tcagctgctt 7793 gccaaaggac cccatccagg tgtaggtttc cacatcgtag gtgaggaaga gcctttctgt 7853 gcgaggatga gagccgatcg ggaagaactg gatctcctgc caccagttgg aggaatggct 7913 gttgatgtga tggaagtaga actccctgcg gcgcgccgag cattcatgct tgtgcttgta 7973 cagacggccg cagtactcgc agcgcttcac gggatgcacc tcatgaatga gttgtacctg 8033 gcttcctttg acgagaaatt tcagtgggaa gttgaggcct ggcgcttgta cctcgcgctc 8093 tactatgtta tttgcatcgg cctggccatc ttctgtctcg atggtggtca tgctgacgag 8153 cccccgcggg aggcaagtcc agacctcggc gcgggagggg cggagctcga ggacgagagc 8213 gcgcaggctg gagctgtcca gggtcctgag acgctgcgga gtcaggttag taggtagtgt 8273 caggagatta acttgcatga tcttttcgag ggcttgcggg aggttcagat ggtacttgat 8333 ctccacgggt ccgttggtgg agatgtcgat ggcttgcagg gtcccgtgcc ccttgggcgc 8393 caccaccgtg cccttgtttt tccttttgtg cggaggtggc tctgttgctt cttgcatgtt 8453 cagaagcggt ggcgagggcg cgcgccgggc ggtaggggcg gctctggccc cggcggcatg 8513 gcaggcagag gcacgtcggc gccgcgcgcg ggtaggttct ggtactgcgc cctgagaaga 8573 cttgcgtgcg cgacgacgcg gcggttgacg tcctggatct gacgcctctg ggtgaaagct 8633 accggacccg tgagcttgaa cctgaaagag agttcaacag aatcaatttc ggtatcgttg 8693 acggcggctt gcctcaggat ctcttgcaca tcgcccgagt tgtcctggta ggcgatctcg 8753 gccatgaact gctcgatttc ttcctcctga agatctccgc ggcccgctct ctcgacggtg 8813 gccgcaaggt cgttggagat gcgacccatg agttgagaga atgcattcat gcccgcctcg 8873 ttccagacgc ggctgtagac cacggccccc tcgggatctc ttgcgcgcat gaccacctgg 8933 gcgaggttga gctccacgtg gcgggtgaag accgcatagt tgcataggcg ctggaagagg 8993 tagttgagtg tggtggcgat gtgctcggtg acgaagaaat acatgatcca tcgtctcagc 9053 ggcatctcgc tgacatcgcc cagggcttcc aagcgctcca tggcctcgta gaagtccaca 9113 gcgaagttga aaaactggga gttgcgcgcg gacacggtca actcctcttc cagaagacgg 9173 atgagatcgg cgatggtggc acgcacctcg cgctcgaagg cccccgggat ttcttcctcc 9233 tcttcttcca ctaacatctc ttcttcctct tcaggcgggg gcggaggagg agggggcacg 9293 cggcgacgcc ggcggcgcac gggcagacgg tcgatgaatc tttcaatgac ctctccgcgg 9353 cggcggcgca tggtctcggt gacggctcgg ccgttctccc tgggtctcag agtgaagacg 9413 cctccgcgca tctccctgaa gtggtgactg gggggctctc cgttgggcag ggacagggca 9473 ctgatgatgc attttatcaa ttgccccgta gggactccgc gcaaggacct gatcgtctga 9533 agatccacgg gatctgaaaa cctttcgacg aaagcgtcta accagtcgca atcgcaaggt 9593 aggctgagca ctgtttcttg cgggcggggg ttctctcttt cttctccttc ctcatcatct 9653 cgggagggtg agacgatgct gctggtgatg aaattaaaat aggcagttct gagacggcgg 9713 atggtggcga ggagcaccag gtctttgggt ccggcttgct ggatgcgcag gcgatcggcc 9773 attccccaag cattatcctg gcatctggcc agatctttat agtagtcttg catgagtcgc 9833 tccacgggca cttcttcttc gcccgctctg ccatgcatgc gcgtgagccc gaacccgcgc 9893 atgggctgga caagtgccag gtcagctacg accctttcgg cgaggatggc ttgctgcacc 9953 tgggtgaggg tggcttggaa gtcgtcaaag tccacgaagc gatggtaggc cccggtgtta 10013 atggtgtagg agcagttggc catgactgac cagttgactg tctggtgccc agggcgaacg 10073 agctcggtgt acttgagtcg cgagtaggcg cgggtgtcaa agatgtaatc gttgcaggtg 10133 cgcaccaggt actggtagcc gatgagaaag tgtggtggtg gctgccggta gaggggccat 10193 cgctctgtag ccggggcacc gggagcgagg tcttccagca tgaggcggtg gtatccgtag 10253 atgtacctgg acatccaggt gatcccggag gcggtggtgg acgcccgcgg gaattcgcgc 10313 actcggttcc agatgttgcg cagcggcatg aagtagttca tggtaggcac ggtctggcca 10373 gtgaggcggg cgcagtcatt gatgctctat agacacggag aaaacgaaag cgatgagcgg 10433 ctcgcctccg tggcctggag gaacgtgaac gggttgggtc gcggtgtacc ccggttcgag 10493 acacaagcca agcgagcaca actcgggccg gccggagccg cggctaacgt ggtattggcg 10553 atcccgtctc gacccagccg acgaatatcc aggatacgga gtcgagtcgt tttgctgctt 10613 gttgcttttt tcctggacgg gtgccagtgc cgcgtcaagc tttagaacgc tcagttcacg 10673 gggccgggag tggctcgcgc ccgtagtctg gagaatcaat cgccagggtt gcgttgcggt 10733 gtgccccggt tcgagcctta gcgtggcccg gatcggccgg tttccgcggc aagcgagggt 10793 ttggcagccc cgtcatttct aagaccccgc cagccgactt ctccagttta cgggagcgag 10853 ccctcttttt ttttgttttt gtcgcccag atg cat ccc gtg ctg cga cag atg 10906 Met His Pro Val Leu Arg Gln Met 635 640 cgc ccc cag caa cag gcc cct tct cag caa cag cag cag cca caa aag 10954 Arg Pro Gln Gln Gln Ala Pro Ser Gln Gln Gln Gln Gln Pro Gln Lys 645 650 655 gct ctt cct gct cct gct cct gca act act gca gcc gca gcc gtg tgc 11002 Ala Leu Pro Ala Pro Ala Pro Ala Thr Thr Ala Ala Ala Ala Val Cys 660 665 670 ggc gcg gga cag tcc gcc tat gat ctg gac ttg gaa gag ggc gag gga 11050 Gly Ala Gly Gln Ser Ala Tyr Asp Leu Asp Leu Glu Glu Gly Glu Gly 675 680 685 ctg gca cgc ctg ggt gca cca tcg ccc gag cgg cac ccg cgg gtg caa 11098 Leu Ala Arg Leu Gly Ala Pro Ser Pro Glu Arg His Pro Arg Val Gln 690 695 700 705 ctg aaa aag gac tct cgc gag gca tac gtg ccc cag cag aac ctg ttc 11146 Leu Lys Lys Asp Ser Arg Glu Ala Tyr Val Pro Gln Gln Asn Leu Phe 710 715 720 agg gac agg agc ggc gag gag ccc gag gag atg cga gcc tct cgc ttt 11194 Arg Asp Arg Ser Gly Glu Glu Pro Glu Glu Met Arg Ala Ser Arg Phe 725 730 735 aac gcg ggt cgc gag ctg cgc cac ggt ctg gac cga aga cgg gtg ctg 11242 Asn Ala Gly Arg Glu Leu Arg His Gly Leu Asp Arg Arg Arg Val Leu 740 745 750 cgg gac gag gat ttc gag gtc gat gaa gtg aca ggg atc agc ccc gct 11290 Arg Asp Glu Asp Phe Glu Val Asp Glu Val Thr Gly Ile Ser Pro Ala 755 760 765 agg gca cat gtg gcc gcg gcc aac ctc gtc tcg gcc tac gag cag acc 11338 Arg Ala His Val Ala Ala Ala Asn Leu Val Ser Ala Tyr Glu Gln Thr 770 775 780 785 gtg aag gag gag cgc aac ttc caa aaa tct ttc aac aac cat gtg cgc 11386 Val Lys Glu Glu Arg Asn Phe Gln Lys Ser Phe Asn Asn His Val Arg 790 795 800 acc ctg atc gcc cgc gag gaa gtg acc ctg ggt ctg atg cac ctg tgg 11434 Thr Leu Ile Ala Arg Glu Glu Val Thr Leu Gly Leu Met His Leu Trp 805 810 815 gac ctg atg gaa gct atc acc cag aac ccc act agc aaa ccc ctg acc 11482 Asp Leu Met Glu Ala Ile Thr Gln Asn Pro Thr Ser Lys Pro Leu Thr 820 825 830 gct cag ctg ttt ctg gtg gtg caa cat agc agg gac aat gag gca ttc 11530 Ala Gln Leu Phe Leu Val Val Gln His Ser Arg Asp Asn Glu Ala Phe 835 840 845 agg gag gcg ctg ctg aac atc acc gag ccc gag ggg aga tgg ttg tat 11578 Arg Glu Ala Leu Leu Asn Ile Thr Glu Pro Glu Gly Arg Trp Leu Tyr 850 855 860 865 gat ctg atc aat atc ctg caa agt att ata gta cag gaa cgt agc ctg 11626 Asp Leu Ile Asn Ile Leu Gln Ser Ile Ile Val Gln Glu Arg Ser Leu 870 875 880 ggt ctg gct gag aaa gtg gca gcc atc aac tac tcg gtc ttg agc ctg 11674 Gly Leu Ala Glu Lys Val Ala Ala Ile Asn Tyr Ser Val Leu Ser Leu 885 890 895 ggc aag tac tac gct cgc aag atc tac aag acc ccc tac gtg ccc ata 11722 Gly Lys Tyr Tyr Ala Arg Lys Ile Tyr Lys Thr Pro Tyr Val Pro Ile 900 905 910 gac aag gag gtg aag ata gat ggg ttt tac atg cgc atg act ctg aag 11770 Asp Lys Glu Val Lys Ile Asp Gly Phe Tyr Met Arg Met Thr Leu Lys 915 920 925 gtg ctg act ctc agt gac gat ctg ggg gtg tac cgc aac gac agg atg 11818 Val Leu Thr Leu Ser Asp Asp Leu Gly Val Tyr Arg Asn Asp Arg Met 930 935 940 945 cac cgc gcg gtg agc gcc agc agg agg cgc gag ctg agc gac aga gaa 11866 His Arg Ala Val Ser Ala Ser Arg Arg Arg Glu Leu Ser Asp Arg Glu 950 955 960 ctt atg cac agc ttg caa aga gct ctg acg ggg gca ggg acc gag ggg 11914 Leu Met His Ser Leu Gln Arg Ala Leu Thr Gly Ala Gly Thr Glu Gly 965 970 975 gag aac tac ttt gac atg gga gcg gac ttg caa tgg cag cct agc cgc 11962 Glu Asn Tyr Phe Asp Met Gly Ala Asp Leu Gln Trp Gln Pro Ser Arg 980 985 990 agg gcc ctg gac gca gca ggg tgt gag ctt cct tac ata gaa gag gtg 12010 Arg Ala Leu Asp Ala Ala Gly Cys Glu Leu Pro Tyr Ile Glu Glu Val 995 1000 1005 gat gaa ggc gag gag gag gag ggc gag tac ctg gaa gac tgatggcgcg 12059 Asp Glu Gly Glu Glu Glu Glu Gly Glu Tyr Leu Glu Asp 1010 1015 1020 acccgtattt ttgctag atg gaa cag cag gca ccg gac ccc gca atg cgg 12109 Met Glu Gln Gln Ala Pro Asp Pro Ala Met Arg 1025 1030 gct gcg ctg cag agc cag ccg tcc ggc att aac tcc tcg gac gat 12154 Ala Ala Leu Gln Ser Gln Pro Ser Gly Ile Asn Ser Ser Asp Asp 1035 1040 1045 tgg acc cag gcc atg caa cgc atc atg gcg ctg acg acc cgc aac 12199 Trp Thr Gln Ala Met Gln Arg Ile Met Ala Leu Thr Thr Arg Asn 1050 1055 1060 ccc gaa gcc ttt aga cag caa ccc cag gcc aac cgc ctt tcg gcc 12244 Pro Glu Ala Phe Arg Gln Gln Pro Gln Ala Asn Arg Leu Ser Ala 1065 1070 1075 atc ctg gag gcc gta gtt cct tcc cga tcc aac ccc acc cac gag 12289 Ile Leu Glu Ala Val Val Pro Ser Arg Ser Asn Pro Thr His Glu 1080 1085 1090 aag gtc ctg gcc atc gtg aac gcg ctg gtg gag aac aag gcc atc 12334 Lys Val Leu Ala Ile Val Asn Ala Leu Val Glu Asn Lys Ala Ile 1095 1100 1105 cgt ccc gat gag gcc ggg ctg gta tac aat gcc ctc ttg gag cgc 12379 Arg Pro Asp Glu Ala Gly Leu Val Tyr Asn Ala Leu Leu Glu Arg 1110 1115 1120 gtg gcc cgc tac aac agc agc aac gtg cag acc aac ctg gac cgg 12424 Val Ala Arg Tyr Asn Ser Ser Asn Val Gln Thr Asn Leu Asp Arg 1125 1130 1135 atg gtg acc gat gtg cgc gag gca gtg tct cag cgc gag cgg ttc 12469 Met Val Thr Asp Val Arg Glu Ala Val Ser Gln Arg Glu Arg Phe 1140 1145 1150 cag cgc gat gcc aac ttg ggg tcg ctg gtg gcg ctg aac gcc ttc 12514 Gln Arg Asp Ala Asn Leu Gly Ser Leu Val Ala Leu Asn Ala Phe 1155 1160 1165 ctc agc acc cag cct gcc aac gtg ccc cgc ggc cag caa gac tat 12559 Leu Ser Thr Gln Pro Ala Asn Val Pro Arg Gly Gln Gln Asp Tyr 1170 1175 1180 aca aac ttc cta agt gca ctg aga ctc atg gta acc gaa gtc cct 12604 Thr Asn Phe Leu Ser Ala Leu Arg Leu Met Val Thr Glu Val Pro 1185 1190 1195 cag agc gag gtg tac cag tcc gga cca gac tac ttc ttc cag acc 12649 Gln Ser Glu Val Tyr Gln Ser Gly Pro Asp Tyr Phe Phe Gln Thr 1200 1205 1210 agc aga cag ggc ttg cag aca gtg aac ctg agc cag gct ttc aaa 12694 Ser Arg Gln Gly Leu Gln Thr Val Asn Leu Ser Gln Ala Phe Lys 1215 1220 1225 aac ctc aga ggc ctg tgg gga gtg cac gcc cca gta gga gat cgc 12739 Asn Leu Arg Gly Leu Trp Gly Val His Ala Pro Val Gly Asp Arg 1230 1235 1240 gcg acc gtg tct agc ttg ctg act ccc aac tcc cgc cta ctg ctg 12784 Ala Thr Val Ser Ser Leu Leu Thr Pro Asn Ser Arg Leu Leu Leu 1245 1250 1255 ctg ctg gta tcc ccc ttc act gac agc ggt agc atc gac cgc aac 12829 Leu Leu Val Ser Pro Phe Thr Asp Ser Gly Ser Ile Asp Arg Asn 1260 1265 1270 tcc tac ttg ggc tac ctg ctg aac ttg tat cgc gag gcc ata ggg 12874 Ser Tyr Leu Gly Tyr Leu Leu Asn Leu Tyr Arg Glu Ala Ile Gly 1275 1280 1285 cag agc cag gtg gac gag cag acc tac caa gaa atc acc caa gtg 12919 Gln Ser Gln Val Asp Glu Gln Thr Tyr Gln Glu Ile Thr Gln Val 1290 1295 1300 agc cgc gcc ctg ggt cag gaa gac acg ggc agc ttg gaa gcc acc 12964 Ser Arg Ala Leu Gly Gln Glu Asp Thr Gly Ser Leu Glu Ala Thr 1305 1310 1315 ctg aac ttc ttg ctg acc aac cgg tcg cag aag atc cct cct cag 13009 Leu Asn Phe Leu Leu Thr Asn Arg Ser Gln Lys Ile Pro Pro Gln 1320 1325 1330 tat gcg ctt acc gcg gag gag gag cgg atc ctc aga tat gtg cag 13054 Tyr Ala Leu Thr Ala Glu Glu Glu Arg Ile Leu Arg Tyr Val Gln 1335 1340 1345 cag agc gtg gga ctg ttc ctg atg cag gag ggg gcg acc cct agt 13099 Gln Ser Val Gly Leu Phe Leu Met Gln Glu Gly Ala Thr Pro Ser 1350 1355 1360 gcc gcg ctg gac atg aca gcg cga aac atg gag ccc agc atg tat 13144 Ala Ala Leu Asp Met Thr Ala Arg Asn Met Glu Pro Ser Met Tyr 1365 1370 1375 gcc agt aac cgg cct ttc att aac aaa ctg ctg gac tac ctg cac 13189 Ala Ser Asn Arg Pro Phe Ile Asn Lys Leu Leu Asp Tyr Leu His 1380 1385 1390 agg gca gcc gct atg aac tct gat tat ttc acc aat gct atc cta 13234 Arg Ala Ala Ala Met Asn Ser Asp Tyr Phe Thr Asn Ala Ile Leu 1395 1400 1405 aac cca cac tgg ctg ccc ccg cct gga ttt tac acg ggc gag tat 13279 Asn Pro His Trp Leu Pro Pro Pro Gly Phe Tyr Thr Gly Glu Tyr 1410 1415 1420 gat atg ccc gac ccc aat gac ggg ttt ctg tgg gac gat gtt gac 13324 Asp Met Pro Asp Pro Asn Asp Gly Phe Leu Trp Asp Asp Val Asp 1425 1430 1435 agc agc ata ttc tcc cca cct cct ggt tat aac act tgg aag aag 13369 Ser Ser Ile Phe Ser Pro Pro Pro Gly Tyr Asn Thr Trp Lys Lys 1440 1445 1450 gaa ggg ggc gat aga aga cac tct tcc gtg tcg ctg tcc ggg tcg 13414 Glu Gly Gly Asp Arg Arg His Ser Ser Val Ser Leu Ser Gly Ser 1455 1460 1465 agg ggt gct gcc gct gcg gtg ccc gag gct gca agt cct ttc cct 13459 Arg Gly Ala Ala Ala Ala Val Pro Glu Ala Ala Ser Pro Phe Pro 1470 1475 1480 agc ctg ccc ttt tct ctg aac agc gtg cgc agc agt gaa ctg ggg 13504 Ser Leu Pro Phe Ser Leu Asn Ser Val Arg Ser Ser Glu Leu Gly 1485 1490 1495 aga ata acc cgc ccg cgc ttg atg ggc gag gat gag tac ttg aac 13549 Arg Ile Thr Arg Pro Arg Leu Met Gly Glu Asp Glu Tyr Leu Asn 1500 1505 1510 gac tcc ttg ctt aga ccc gag agg gaa aag aac ttc ccc aac aat 13594 Asp Ser Leu Leu Arg Pro Glu Arg Glu Lys Asn Phe Pro Asn Asn 1515 1520 1525 ggg ata gag agc ctg gtg gat aag atg agt aga tgg aag acc tat 13639 Gly Ile Glu Ser Leu Val Asp Lys Met Ser Arg Trp Lys Thr Tyr 1530 1535 1540 gca cag gat cac aaa gat gag cct agg atc ttg gga gct gca agc 13684 Ala Gln Asp His Lys Asp Glu Pro Arg Ile Leu Gly Ala Ala Ser 1545 1550 1555 ggg acg acc cgt aga cgc cag cgc cat gac agg cag agg ggt ctt 13729 Gly Thr Thr Arg Arg Arg Gln Arg His Asp Arg Gln Arg Gly Leu 1560 1565 1570 gta tgg gac gat gag gac tcg gcc gat gac agc agc gtg ttg gac 13774 Val Trp Asp Asp Glu Asp Ser Ala Asp Asp Ser Ser Val Leu Asp 1575 1580 1585 ttg ggt ggg aga gga ggt ggc aac ccg ttc gct cat ctg cgc ccg 13819 Leu Gly Gly Arg Gly Gly Gly Asn Pro Phe Ala His Leu Arg Pro 1590 1595 1600 cac ttt ggg cgc atg ttg taaaagtgaa agtaaaataa aaaaggcaac 13867 His Phe Gly Arg Met Leu 1605 tcaccaaggc catggcgacg agcgtgcgtt cgttcttttc tgttatct atg tct agt 13924 Met Ser Ser 1610 atg atg agg cga gcc gtg cta ggc gga gcg gtg gtg tat ccg gag 13969 Met Met Arg Arg Ala Val Leu Gly Gly Ala Val Val Tyr Pro Glu 1615 1620 1625 ggt cct cct cct tcg tac gag agc gtg atg cag cag cag gcg gcg 14014 Gly Pro Pro Pro Ser Tyr Glu Ser Val Met Gln Gln Gln Ala Ala 1630 1635 1640 gcg gtg atg cag ccc tcg ctg gag gct ccc ttt gta ccc ccg cgg 14059 Ala Val Met Gln Pro Ser Leu Glu Ala Pro Phe Val Pro Pro Arg 1645 1650 1655 tac ctg gcg cct aca gag ggg aga aac agc att cgt tac tcg gag 14104 Tyr Leu Ala Pro Thr Glu Gly Arg Asn Ser Ile Arg Tyr Ser Glu 1660 1665 1670 ctg gca ccc cag tac gat acc acc agg ttg tat ctg gtg gac aac 14149 Leu Ala Pro Gln Tyr Asp Thr Thr Arg Leu Tyr Leu Val Asp Asn 1675 1680 1685 aag tcg gcg gac atc gcc tca ttg aac tat cag aac gac cac agc 14194 Lys Ser Ala Asp Ile Ala Ser Leu Asn Tyr Gln Asn Asp His Ser 1690 1695 1700 aac ttc ctg acc acg gtg gtg cag aac aat gac ttt acc ccc acg 14239 Asn Phe Leu Thr Thr Val Val Gln Asn Asn Asp Phe Thr Pro Thr 1705 1710 1715 gag gcc agc acc cag acc atc aac ttt gac gag cgg tcg cgg tgg 14284 Glu Ala Ser Thr Gln Thr Ile Asn Phe Asp Glu Arg Ser Arg Trp 1720 1725 1730 ggc ggt cag ctg aag acc atc atg cac acc aac atg ccc aac gta 14329 Gly Gly Gln Leu Lys Thr Ile Met His Thr Asn Met Pro Asn Val 1735 1740 1745 aac gag tac atg ttc agt aac aag ttc aag gcg cgg gtg atg gtg 14374 Asn Glu Tyr Met Phe Ser Asn Lys Phe Lys Ala Arg Val Met Val 1750 1755 1760 tcc aga aag gct cct gaa ggt gtt aca gta gat gac acc tat gat 14419 Ser Arg Lys Ala Pro Glu Gly Val Thr Val Asp Asp Thr Tyr Asp 1765 1770 1775 cat aag cag gat ata ctg gag tat gag tgg ttt gag ttc act ctg 14464 His Lys Gln Asp Ile Leu Glu Tyr Glu Trp Phe Glu Phe Thr Leu 1780 1785 1790 cca gaa ggc aac ttc tca gcc acc atg acc atc gac ctg atg aac 14509 Pro Glu Gly Asn Phe Ser Ala Thr Met Thr Ile Asp Leu Met Asn 1795 1800 1805 aat gcc atc att gac aac tac ctg gaa att gga aga cag aat gga 14554 Asn Ala Ile Ile Asp Asn Tyr Leu Glu Ile Gly Arg Gln Asn Gly 1810 1815 1820 gtg ctg gaa agt gac att ggt gtc aag ttt gat acc aga aac ttc 14599 Val Leu Glu Ser Asp Ile Gly Val Lys Phe Asp Thr Arg Asn Phe 1825 1830 1835 agg ctt ggc tgg gac ccc gaa act aag tta att atg cct ggg gtt 14644 Arg Leu Gly Trp Asp Pro Glu Thr Lys Leu Ile Met Pro Gly Val 1840 1845 1850 tac acc tat gag gcc ttc cat cct gac att gtg cta ctg cct ggt 14689 Tyr Thr Tyr Glu Ala Phe His Pro Asp Ile Val Leu Leu Pro Gly 1855 1860 1865 tgc ggg gtg gac ttc act gaa agc cgc ctt agc aac ttg ctt gga 14734 Cys Gly Val Asp Phe Thr Glu Ser Arg Leu Ser Asn Leu Leu Gly 1870 1875 1880 atc agg aag aga cac cca ttc cag gaa ggc ttt cag ata ctg tat 14779 Ile Arg Lys Arg His Pro Phe Gln Glu Gly Phe Gln Ile Leu Tyr 1885 1890 1895 gaa gat ctc gaa ggg ggt aat atc ccc gcc ctt ctg gat gta gaa 14824 Glu Asp Leu Glu Gly Gly Asn Ile Pro Ala Leu Leu Asp Val Glu 1900 1905 1910 acc tat gag aaa agc aaa aag gaa aat gca ggc acc act act gaa 14869 Thr Tyr Glu Lys Ser Lys Lys Glu Asn Ala Gly Thr Thr Thr Glu 1915 1920 1925 ggg aca aca act gtc gct gtt gct aat gca cta acg aca gct aaa 14914 Gly Thr Thr Thr Val Ala Val Ala Asn Ala Leu Thr Thr Ala Lys 1930 1935 1940 gcg gca gca aat gtg aca gta gat gtt att act gaa ata aac aat 14959 Ala Ala Ala Asn Val Thr Val Asp Val Ile Thr Glu Ile Asn Asn 1945 1950 1955 aat tcg gtt aga gga gat aat tac cta tct gct aat gac atg aaa 15004 Asn Ser Val Arg Gly Asp Asn Tyr Leu Ser Ala Asn Asp Met Lys 1960 1965 1970 gac tct agt gaa aca act gtg gag ccg gca gtt cct att gta gta 15049 Asp Ser Ser Glu Thr Thr Val Glu Pro Ala Val Pro Ile Val Val 1975 1980 1985 cct gga act aaa act gaa act gaa acc gaa acc aaa aca cca acc 15094 Pro Gly Thr Lys Thr Glu Thr Glu Thr Glu Thr Lys Thr Pro Thr 1990 1995 2000 atc cag ccg cta aaa aaa gat agc aaa agt agg agt tat aat gtc 15139 Ile Gln Pro Leu Lys Lys Asp Ser Lys Ser Arg Ser Tyr Asn Val 2005 2010 2015 ttg gaa gat gaa gtc aac aca gcc tat cgc agt tgg tac ttg tcc 15184 Leu Glu Asp Glu Val Asn Thr Ala Tyr Arg Ser Trp Tyr Leu Ser 2020 2025 2030 tac aac tat ggc gac cct gaa aaa gga gtc cgc tcc tgg aca ctg 15229 Tyr Asn Tyr Gly Asp Pro Glu Lys Gly Val Arg Ser Trp Thr Leu 2035 2040 2045 ctc acc act tca gat gtc acc tgc gga gcg gag caa gtc tat tgg 15274 Leu Thr Thr Ser Asp Val Thr Cys Gly Ala Glu Gln Val Tyr Trp 2050 2055 2060 tcc ctc cct gac atg atg cag gac ccc gtc acc ttc cgc tct acg 15319 Ser Leu Pro Asp Met Met Gln Asp Pro Val Thr Phe Arg Ser Thr 2065 2070 2075 aga caa gtc agc aac tac ccc gtg gtg ggt gca gag ctc atg ccc 15364 Arg Gln Val Ser Asn Tyr Pro Val Val Gly Ala Glu Leu Met Pro 2080 2085 2090 gtc ttc tca aag agt ttc tac aac gag caa gcc gtg tac tcc cag 15409 Val Phe Ser Lys Ser Phe Tyr Asn Glu Gln Ala Val Tyr Ser Gln 2095 2100 2105 cag ctc cgc cag acc acc tcg ctc acg cac gtc ttc aat cgc ttc 15454 Gln Leu Arg Gln Thr Thr Ser Leu Thr His Val Phe Asn Arg Phe 2110 2115 2120 cct gag aat cag atc ctc atc cgc ccg ccg gcg ccc acc att acc 15499 Pro Glu Asn Gln Ile Leu Ile Arg Pro Pro Ala Pro Thr Ile Thr 2125 2130 2135 acc gtt agt gaa aac gtt cct gct ctc aca gat cac ggg acc ctg 15544 Thr Val Ser Glu Asn Val Pro Ala Leu Thr Asp His Gly Thr Leu 2140 2145 2150 ccg ttg cgc agc agt atc cgg gga gtc cag cgc gtg acc gtt act 15589 Pro Leu Arg Ser Ser Ile Arg Gly Val Gln Arg Val Thr Val Thr 2155 2160 2165 gac gcc aga cgc cgc acc tgc ccc tac gtc tac aag gcc ctg ggc 15634 Asp Ala Arg Arg Arg Thr Cys Pro Tyr Val Tyr Lys Ala Leu Gly 2170 2175 2180 ata gtc gcg ccg cgc gtc ctt tca agc cgc act ttc taaaaaaaaa aa 15682 Ile Val Ala Pro Arg Val Leu Ser Ser Arg Thr Phe 2185 2190 atg tcc att ctc atc tca ccc agt aat aac acc ggt tgg ggg ctg 15727 Met Ser Ile Leu Ile Ser Pro Ser Asn Asn Thr Gly Trp Gly Leu 2195 2200 2205 cgc aca ccc acc agg atg tac gga ggc gct cgc aaa cgg tct acc 15772 Arg Thr Pro Thr Arg Met Tyr Gly Gly Ala Arg Lys Arg Ser Thr 2210 2215 2220 cag cac cct gtg cgt gta cgc ggg cat ttc cgc gct ccc tgg ggc 15817 Gln His Pro Val Arg Val Arg Gly His Phe Arg Ala Pro Trp Gly 2225 2230 2235 gcc ctc aag ggc cgt gct cgc act cgg acc acc gtc gat gat gtg 15862 Ala Leu Lys Gly Arg Ala Arg Thr Arg Thr Thr Val Asp Asp Val 2240 2245 2250 atc gac cag gtg gtt gca gat gct cgt aat tat act cct gct gca 15907 Ile Asp Gln Val Val Ala Asp Ala Arg Asn Tyr Thr Pro Ala Ala 2255 2260 2265 cct gca tct act gtg gat gca gtt att gac agc gtg gtg gct gac 15952 Pro Ala Ser Thr Val Asp Ala Val Ile Asp Ser Val Val Ala Asp 2270 2275 2280 gct cgc gac tat gct cgc agg aag agc agg cga aga cgc att gcc 15997 Ala Arg Asp Tyr Ala Arg Arg Lys Ser Arg Arg Arg Arg Ile Ala 2285 2290 2295 agg cgc cac cgg act acc ccc gcc atg cga gct gca aga gct ctg 16042 Arg Arg His Arg Thr Thr Pro Ala Met Arg Ala Ala Arg Ala Leu 2300 2305 2310 ctg cgg aaa gcc aaa cgc gtg ggg cga aga gcc atg ctt aga gcg 16087 Leu Arg Lys Ala Lys Arg Val Gly Arg Arg Ala Met Leu Arg Ala 2315 2320 2325 gcc aga cgc gcg gct tca ggt gcc atc gca ggc agg tcc cgc agg 16132 Ala Arg Arg Ala Ala Ser Gly Ala Ile Ala Gly Arg Ser Arg Arg 2330 2335 2340 cgc gcg gcc acg gcg gca gca gcg gcc att gcc aac atg gcc caa 16177 Arg Ala Ala Thr Ala Ala Ala Ala Ala Ile Ala Asn Met Ala Gln 2345 2350 2355 ccg cga aga ggc aat gtg tac tgg gtg cgc gat gcc act acc ggc 16222 Pro Arg Arg Gly Asn Val Tyr Trp Val Arg Asp Ala Thr Thr Gly 2360 2365 2370 cag cgc gtg ccc gtg cgc acc cgt ccc cct cgc act tagaagatac 16268 Gln Arg Val Pro Val Arg Thr Arg Pro Pro Arg Thr 2375 2380 2385 tgagcagtct ccgatgttgt gtcccagcgg cgagg atg tcc aag cgc aaa tac 16321 Met Ser Lys Arg Lys Tyr 2390 aag gaa gag atg ctc cag gtc atc gcg cct gaa atc tac ggt cca 16366 Lys Glu Glu Met Leu Gln Val Ile Ala Pro Glu Ile Tyr Gly Pro 2395 2400 2405 ccg gtg aag gat gaa aaa aag ccc cgc aaa atc aag cgg gtc aaa 16411 Pro Val Lys Asp Glu Lys Lys Pro Arg Lys Ile Lys Arg Val Lys 2410 2415 2420 aag gac aaa aag gaa gaa gat ggc gat gat ggg ctg gtg gag ttt 16456 Lys Asp Lys Lys Glu Glu Asp Gly Asp Asp Gly Leu Val Glu Phe 2425 2430 2435 gtg cgc gag ttc gct cca agg cgg cgc gtg cag tgg cgc ggg cgc 16501 Val Arg Glu Phe Ala Pro Arg Arg Arg Val Gln Trp Arg Gly Arg 2440 2445 2450 agg gtg cgg ccg gtg ctg aga ccc gga acc acg gtg gtc ttc acg 16546 Arg Val Arg Pro Val Leu Arg Pro Gly Thr Thr Val Val Phe Thr 2455 2460 2465 ccc ggc gag cgc tcc agc act act ttt aaa cgc tct tat gat gag 16591 Pro Gly Glu Arg Ser Ser Thr Thr Phe Lys Arg Ser Tyr Asp Glu 2470 2475 2480 gtg tac ggt gac gat gat att ctg gag cag gca gcc gac cgc ctg 16636 Val Tyr Gly Asp Asp Asp Ile Leu Glu Gln Ala Ala Asp Arg Leu 2485 2490 2495 ggc gag ttt gct tat ggc aaa cgc agc cgc tct agt ccc aag gag 16681 Gly Glu Phe Ala Tyr Gly Lys Arg Ser Arg Ser Ser Pro Lys Glu 2500 2505 2510 gag gcg gtg tcc atc ccc ttg gat cat gga aat ccc acc ccg agt 16726 Glu Ala Val Ser Ile Pro Leu Asp His Gly Asn Pro Thr Pro Ser 2515 2520 2525 ctc aaa cca gtc acc ctg cag caa gtg ctg ccc gtg cct cca cgg 16771 Leu Lys Pro Val Thr Leu Gln Gln Val Leu Pro Val Pro Pro Arg 2530 2535 2540 aga ggt gtc aag cga gag ggc gag gat ctg tat ccc acc atg caa 16816 Arg Gly Val Lys Arg Glu Gly Glu Asp Leu Tyr Pro Thr Met Gln 2545 2550 2555 ttg atg gtg ccc aag cgc cag aag ctg gag gac gtg ctg gag aaa 16861 Leu Met Val Pro Lys Arg Gln Lys Leu Glu Asp Val Leu Glu Lys 2560 2565 2570 atg aaa gtg gat ccc gat atc cag cct gaa gta aaa gtc aga ccc 16906 Met Lys Val Asp Pro Asp Ile Gln Pro Glu Val Lys Val Arg Pro 2575 2580 2585 atc aag cag gtg gcg ccc ggt ctg gga gta caa acc gtg gac atc 16951 Ile Lys Gln Val Ala Pro Gly Leu Gly Val Gln Thr Val Asp Ile 2590 2595 2600 aag att ccc acc gag tcc atg gaa gtc cag act gaa cct gca aag 16996 Lys Ile Pro Thr Glu Ser Met Glu Val Gln Thr Glu Pro Ala Lys 2605 2610 2615 cct aca gcc acc tcc att gag gtg cag aca gat cca tgg atg ccc 17041 Pro Thr Ala Thr Ser Ile Glu Val Gln Thr Asp Pro Trp Met Pro 2620 2625 2630 gcg ccc att gca acc acc gcc agt acc gct cga aga ccc cgg cga 17086 Ala Pro Ile Ala Thr Thr Ala Ser Thr Ala Arg Arg Pro Arg Arg 2635 2640 2645 aag tat ggt cca gcg agt ctg ctg atg ccc aac tat gct ctg cac 17131 Lys Tyr Gly Pro Ala Ser Leu Leu Met Pro Asn Tyr Ala Leu His 2650 2655 2660 cca tcc att att cca act ccg ggt tac cga ggc act cgc tac tac 17176 Pro Ser Ile Ile Pro Thr Pro Gly Tyr Arg Gly Thr Arg Tyr Tyr 2665 2670 2675 cgc agc cgg agc acc act tcc cgc cgt cgc aaa aca cct gca agc 17221 Arg Ser Arg Ser Thr Thr Ser Arg Arg Arg Lys Thr Pro Ala Ser 2680 2685 2690 cgc agc cgc cgt cgc cgc cgc cgc gcc acc agc aaa ctg act ccc 17266 Arg Ser Arg Arg Arg Arg Arg Arg Ala Thr Ser Lys Leu Thr Pro 2695 2700 2705 gcc gct ctg gtg cgg agg gtg tat cgc gat ggc cgc gca gag ccc 17311 Ala Ala Leu Val Arg Arg Val Tyr Arg Asp Gly Arg Ala Glu Pro 2710 2715 2720 ctg atg ctg ccg cgc gca cgc tac cat cca agc atc acc act 17353 Leu Met Leu Pro Arg Ala Arg Tyr His Pro Ser Ile Thr Thr 2725 2730 2735 taatgactgt tgccactgcc tccttgcaga t atg gcc ctc act tgc cgc ctt 17405 Met Ala Leu Thr Cys Arg Leu 2740 cgc gtc ccc att act ggc tac cga gga aga aac tcg cgc cgt aga 17450 Arg Val Pro Ile Thr Gly Tyr Arg Gly Arg Asn Ser Arg Arg Arg 2745 2750 2755 agg atg ttg ggg cgc ggg atg cgt cgc cac agg cgg cgg cgc gct 17495 Arg Met Leu Gly Arg Gly Met Arg Arg His Arg Arg Arg Arg Ala 2760 2765 2770 atc agc aag agg ctg ggg ggt ggc ttt ctg acc gct ttg att ccc 17540 Ile Ser Lys Arg Leu Gly Gly Gly Phe Leu Thr Ala Leu Ile Pro 2775 2780 2785 atc atc gcc gcg gcg att ggg gca gta cca ggc ata gct tcc gtg 17585 Ile Ile Ala Ala Ala Ile Gly Ala Val Pro Gly Ile Ala Ser Val 2790 2795 2800 gcg gtt cag gcc tcg cag cgc cac tgacattgga aaaaaactta 17629 Ala Val Gln Ala Ser Gln Arg His 2805 2810 taaataaaat agaatggact ctgacgctcc tggtcctgtg actatgtttt tgtagag 17686 atg gaa gac atc aat ttt tca tcc ctg gct ccg cga cac ggc acg 17731 Met Glu Asp Ile Asn Phe Ser Ser Leu Ala Pro Arg His Gly Thr 2815 2820 2825 agg ccg tac atg ggc acc tgg agc gac atc ggc acc agc caa ctg 17776 Arg Pro Tyr Met Gly Thr Trp Ser Asp Ile Gly Thr Ser Gln Leu 2830 2835 2840 aac ggg ggc gcc ttc aat tgg agc agt atc tgg agc ggg ctt aaa 17821 Asn Gly Gly Ala Phe Asn Trp Ser Ser Ile Trp Ser Gly Leu Lys 2845 2850 2855 aat ttt ggc tct acc ata aaa acc tat ggg aac aaa gct tgg aac 17866 Asn Phe Gly Ser Thr Ile Lys Thr Tyr Gly Asn Lys Ala Trp Asn 2860 2865 2870 agc agc aca ggg cag gcg ctg agg aat aag ctt aaa gag caa aac 17911 Ser Ser Thr Gly Gln Ala Leu Arg Asn Lys Leu Lys Glu Gln Asn 2875 2880 2885 ttc cag cag aag gtg gtc gat ggg atc gcc tct ggt atc aat ggg 17956 Phe Gln Gln Lys Val Val Asp Gly Ile Ala Ser Gly Ile Asn Gly 2890 2895 2900 gtg gtg gat ctg gcc aac cag gcc gtg cag aaa cag ata aac agc 18001 Val Val Asp Leu Ala Asn Gln Ala Val Gln Lys Gln Ile Asn Ser 2905 2910 2915 cgc ctg gac ccg ccg cct gca gcc cct ggc gaa atg gaa gtg gag 18046 Arg Leu Asp Pro Pro Pro Ala Ala Pro Gly Glu Met Glu Val Glu 2920 2925 2930 gaa gag ctc cct ccc ctg gaa aag cgg gga gac aag cgc ccg cgt 18091 Glu Glu Leu Pro Pro Leu Glu Lys Arg Gly Asp Lys Arg Pro Arg 2935 2940 2945 ccc gat atg gag gag acg ctg gtg acg cgc gga gac gag ccg cct 18136 Pro Asp Met Glu Glu Thr Leu Val Thr Arg Gly Asp Glu Pro Pro 2950 2955 2960 cca tac gag gag gcg ata aag ctt gga atg ccc act acc agg cct 18181 Pro Tyr Glu Glu Ala Ile Lys Leu Gly Met Pro Thr Thr Arg Pro 2965 2970 2975 ata gct ccc atg gcc acc ggg gta atg aaa cct tct cag tcg cat 18226 Ile Ala Pro Met Ala Thr Gly Val Met Lys Pro Ser Gln Ser His 2980 2985 2990 cga cct gcc acc ttg gac ttg cct cct gcc cct gct gct gca gcg 18271 Arg Pro Ala Thr Leu Asp Leu Pro Pro Ala Pro Ala Ala Ala Ala 2995 3000 3005 ccc gct cca aag cct gtc gct acc ccg aag ccc acc acc gta cag 18316 Pro Ala Pro Lys Pro Val Ala Thr Pro Lys Pro Thr Thr Val Gln 3010 3015 3020 ccc gtc gcc gta gcc aga ccg cgt ccc ggg ggc act ccg cgc ccg 18361 Pro Val Ala Val Ala Arg Pro Arg Pro Gly Gly Thr Pro Arg Pro 3025 3030 3035 aat gca aac tgg cag agt act ctg aac agc atc gtg ggt ctg ggc 18406 Asn Ala Asn Trp Gln Ser Thr Leu Asn Ser Ile Val Gly Leu Gly 3040 3045 3050 gtg cag agt gta aag cgc cgt cgc tgc tat taattaaatg tggagtagcg 18456 Val Gln Ser Val Lys Arg Arg Arg Cys Tyr 3055 3060 cttaacttgc ttgtctgtgt gtatgtgtca tcaccacgcc gccgcagcag cagaggagaa 18516 aggaagaggt cgcgcgccga ggctgagttg ctttcaag atg gcc acc cca tcg 18569 Met Ala Thr Pro Ser 3065 atg ctg ccc cag tgg gca tac atg cac atc gcc gga cag gat gct 18614 Met Leu Pro Gln Trp Ala Tyr Met His Ile Ala Gly Gln Asp Ala 3070 3075 3080 tcg gag tac ctg agt ccg ggt ctg gtg cag ttc gcc cgt gcc aca 18659 Ser Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala Arg Ala Thr 3085 3090 3095 gac acc tac ttc aat ctg ggg aac aag ttt agg aac ccc acc gtg 18704 Asp Thr Tyr Phe Asn Leu Gly Asn Lys Phe Arg Asn Pro Thr Val 3100 3105 3110 gcc ccc acc cac gat gtg acc acc gac cga agc cag cgg ctg atg 18749 Ala Pro Thr His Asp Val Thr Thr Asp Arg Ser Gln Arg Leu Met 3115 3120 3125 ctg cgc ttt gtg ccc gtt gat cgg gag gac aat act tac tct tac 18794 Leu Arg Phe Val Pro Val Asp Arg Glu Asp Asn Thr Tyr Ser Tyr 3130 3135 3140 aaa gtt cgc tac aca ctg gct gtg ggc gac aac aga gtg ctg gat 18839 Lys Val Arg Tyr Thr Leu Ala Val Gly Asp Asn Arg Val Leu Asp 3145 3150 3155 atg gcc agc acc ttc ttt gac att cgg ggc gtg ctt gac aga ggt 18884 Met Ala Ser Thr Phe Phe Asp Ile Arg Gly Val Leu Asp Arg Gly 3160 3165 3170 cct agc ttc aag cca tac tct ggc aca gct tac aac tcc cta gca 18929 Pro Ser Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser Leu Ala 3175 3180 3185 cct aag gga gcc ccc aat aca tct cag tgg ctt gcc gaa ggg aca 18974 Pro Lys Gly Ala Pro Asn Thr Ser Gln Trp Leu Ala Glu Gly Thr 3190 3195 3200 aat aat gct gca gag ggg gag gct gaa caa gat gag gag gac ggg 19019 Asn Asn Ala Ala Glu Gly Glu Ala Glu Gln Asp Glu Glu Asp Gly 3205 3210 3215 ggc gaa gaa gaa aca aaa atg gct aca tac act ttt ggc aat gct 19064 Gly Glu Glu Glu Thr Lys Met Ala Thr Tyr Thr Phe Gly Asn Ala 3220 3225 3230 cca gta aaa gct gac gct gaa att aca aag gaa ggg ttg gca gta 19109 Pro Val Lys Ala Asp Ala Glu Ile Thr Lys Glu Gly Leu Ala Val 3235 3240 3245 gga gta gag cta tta gct gat aac aac act aaa cca att tat gca 19154 Gly Val Glu Leu Leu Ala Asp Asn Asn Thr Lys Pro Ile Tyr Ala 3250 3255 3260 gat aaa ctg tat caa cct gaa ccc caa gtt gga gag gaa act tgg 19199 Asp Lys Leu Tyr Gln Pro Glu Pro Gln Val Gly Glu Glu Thr Trp 3265 3270 3275 act gat aca gac ggg aca aat gaa cag tat gga ggc agg gct tta 19244 Thr Asp Thr Asp Gly Thr Asn Glu Gln Tyr Gly Gly Arg Ala Leu 3280 3285 3290 aag cct gag act aag atg aaa cca tgc tac gga tct ttt gcc agg 19289 Lys Pro Glu Thr Lys Met Lys Pro Cys Tyr Gly Ser Phe Ala Arg 3295 3300 3305 cca acc aat act aaa ggc ggt cag gca aaa cta aaa aac cca gac 19334 Pro Thr Asn Thr Lys Gly Gly Gln Ala Lys Leu Lys Asn Pro Asp 3310 3315 3320 gaa aaa gac ata acc aaa att gaa tat gat gtt gag atg gac ttt 19379 Glu Lys Asp Ile Thr Lys Ile Glu Tyr Asp Val Glu Met Asp Phe 3325 3330 3335 tat gag ctt aaa tcg cag gta aat ggc agt cca aaa att gtg atg 19424 Tyr Glu Leu Lys Ser Gln Val Asn Gly Ser Pro Lys Ile Val Met 3340 3345 3350 tat gca gaa aat gta aat cta gaa act cca gac act cac gtg gtg 19469 Tyr Ala Glu Asn Val Asn Leu Glu Thr Pro Asp Thr His Val Val 3355 3360 3365 tac aag cct gga act tca gat gat agt tct cat gcc aat ctt ggt 19514 Tyr Lys Pro Gly Thr Ser Asp Asp Ser Ser His Ala Asn Leu Gly 3370 3375 3380 caa caa tcc atg ccc aat aga cct aac tac att ggc ttc aga gac 19559 Gln Gln Ser Met Pro Asn Arg Pro Asn Tyr Ile Gly Phe Arg Asp 3385 3390 3395 aac ttc att ggt ctc gca tac tat aac agt act ggc aat atg gga 19604 Asn Phe Ile Gly Leu Ala Tyr Tyr Asn Ser Thr Gly Asn Met Gly 3400 3405 3410 gta ctg gct ggg cag gca tca cag cta aat gca gtg gtt gac tta 19649 Val Leu Ala Gly Gln Ala Ser Gln Leu Asn Ala Val Val Asp Leu 3415 3420 3425 caa gac aga aac acc gaa ttg tca tac caa cta ctg ctt gac tct 19694 Gln Asp Arg Asn Thr Glu Leu Ser Tyr Gln Leu Leu Leu Asp Ser 3430 3435 3440 ttg ggc gac aga acc agg tac ttt agc atg tgg aac cag gcc gtg 19739 Leu Gly Asp Arg Thr Arg Tyr Phe Ser Met Trp Asn Gln Ala Val 3445 3450 3455 gat agc tat gat ccc gat gta cgc att att gaa aat cat ggt gtg 19784 Asp Ser Tyr Asp Pro Asp Val Arg Ile Ile Glu Asn His Gly Val 3460 3465 3470 gaa gac gaa ctt ccc aat tat tgc ttt cca ctg aat ggc gtt ggt 19829 Glu Asp Glu Leu Pro Asn Tyr Cys Phe Pro Leu Asn Gly Val Gly 3475 3480 3485 tcg gaa aca gaa aga tac aaa gaa atg caa gct aaa aat ggg aat 19874 Ser Glu Thr Glu Arg Tyr Lys Glu Met Gln Ala Lys Asn Gly Asn 3490 3495 3500 gaa aat gga tgg gat aat gct aat cct act gga aca agt gag att 19919 Glu Asn Gly Trp Asp Asn Ala Asn Pro Thr Gly Thr Ser Glu Ile 3505 3510 3515 gct aaa ggc aat ccc tat gct atg gaa att aat ctt cag gct aac 19964 Ala Lys Gly Asn Pro Tyr Ala Met Glu Ile Asn Leu Gln Ala Asn 3520 3525 3530 ctt tgg aga agt ttt ctt tat tct aat gtt gct ttg tac ctc cct 20009 Leu Trp Arg Ser Phe Leu Tyr Ser Asn Val Ala Leu Tyr Leu Pro 3535 3540 3545 gat tct tac aaa tac act cca gct aat atc act ctc cct tcc aac 20054 Asp Ser Tyr Lys Tyr Thr Pro Ala Asn Ile Thr Leu Pro Ser Asn 3550 3555 3560 act aac act tat gac tac ctg aac ggg cgg gtg gtt ccc ccc tcc 20099 Thr Asn Thr Tyr Asp Tyr Leu Asn Gly Arg Val Val Pro Pro Ser 3565 3570 3575 cta gtg gat aca tat ata aac att ggc gcc aga tgg tct ctg gat 20144 Leu Val Asp Thr Tyr Ile Asn Ile Gly Ala Arg Trp Ser Leu Asp 3580 3585 3590 gcc atg gac aat gtc aac cca ttc aac cac cac cgc aac gct gga 20189 Ala Met Asp Asn Val Asn Pro Phe Asn His His Arg Asn Ala Gly 3595 3600 3605 ttg cgc tac cgg tcc atg ctt ttg ggc aat ggt cgc tat gtg cct 20234 Leu Arg Tyr Arg Ser Met Leu Leu Gly Asn Gly Arg Tyr Val Pro 3610 3615 3620 ttc cac atc caa gtg cca caa aaa ttc ttt gcc gtc aag aac ctg 20279 Phe His Ile Gln Val Pro Gln Lys Phe Phe Ala Val Lys Asn Leu 3625 3630 3635 ctg ctt ctc cct ggt tcc tac acc tat gag tgg aac ttc aga aag 20324 Leu Leu Leu Pro Gly Ser Tyr Thr Tyr Glu Trp Asn Phe Arg Lys 3640 3645 3650 gac gtg aac atg gtt ttg cag agt tca ctt ggc aac gac ctc cgg 20369 Asp Val Asn Met Val Leu Gln Ser Ser Leu Gly Asn Asp Leu Arg 3655 3660 3665 gtc gac ggt gcc agc atc agt ttt acc agc atc aac ctc tat gct 20414 Val Asp Gly Ala Ser Ile Ser Phe Thr Ser Ile Asn Leu Tyr Ala 3670 3675 3680 acc ttt ttc ccc atg gct cac aac act gct tcc acc ctt gaa gcc 20459 Thr Phe Phe Pro Met Ala His Asn Thr Ala Ser Thr Leu Glu Ala 3685 3690 3695 atg ttg cgc aat gac acc aat gac cag tca ttc aat gac tac ctc 20504 Met Leu Arg Asn Asp Thr Asn Asp Gln Ser Phe Asn Asp Tyr Leu 3700 3705 3710 tct gcg gct aac atg ctc tac ccc att cca gca aat gcc acc aac 20549 Ser Ala Ala Asn Met Leu Tyr Pro Ile Pro Ala Asn Ala Thr Asn 3715 3720 3725 att ccc atc tcc att ccc tct cgc aac tgg gct gcc ttt agg gga 20594 Ile Pro Ile Ser Ile Pro Ser Arg Asn Trp Ala Ala Phe Arg Gly 3730 3735 3740 tgg tca ttc acc aga ctc aaa acc aag gaa aca ccc tct ttg gga 20639 Trp Ser Phe Thr Arg Leu Lys Thr Lys Glu Thr Pro Ser Leu Gly 3745 3750 3755 tca ggc ttt gat ccc tac ttt gtt tac tct ggc tct att ccc tac 20684 Ser Gly Phe Asp Pro Tyr Phe Val Tyr Ser Gly Ser Ile Pro Tyr 3760 3765 3770 ctg gat ggt acc ttc tac ctc aac cac act ttc aag aag gtg tcc 20729 Leu Asp Gly Thr Phe Tyr Leu Asn His Thr Phe Lys Lys Val Ser 3775 3780 3785 atc atg ttt gac tcc tca gtc agc tgg cct ggc aat gac aga ctg 20774 Ile Met Phe Asp Ser Ser Val Ser Trp Pro Gly Asn Asp Arg Leu 3790 3795 3800 cta tct cca aat gag ttt gaa atc aag cgc act gtg gat ggg gaa 20819 Leu Ser Pro Asn Glu Phe Glu Ile Lys Arg Thr Val Asp Gly Glu 3805 3810 3815 ggg tac aat gtg gct caa tgc aac atg acc aag gac tgg ttc ctg 20864 Gly Tyr Asn Val Ala Gln Cys Asn Met Thr Lys Asp Trp Phe Leu 3820 3825 3830 gtt cag atg ctg gcc aac tac aac att ggc tac cag ggc ttc tac 20909 Val Gln Met Leu Ala Asn Tyr Asn Ile Gly Tyr Gln Gly Phe Tyr 3835 3840 3845 att cca gaa gga tac aag gat cgc atg tat tcc ttc ttt aga aac 20954 Ile Pro Glu Gly Tyr Lys Asp Arg Met Tyr Ser Phe Phe Arg Asn 3850 3855 3860 ttc cag ccc atg agc aga cag gtg gtt gat gag gtt aac tac act 20999 Phe Gln Pro Met Ser Arg Gln Val Val Asp Glu Val Asn Tyr Thr 3865 3870 3875 gac tac aag gct gtt gcc gtt cct tac cag cac aac aat tct ggt 21044 Asp Tyr Lys Ala Val Ala Val Pro Tyr Gln His Asn Asn Ser Gly 3880 3885 3890 ttt gtg ggt tac atg gct cct aca atg cgc cag gga caa gct tat 21089 Phe Val Gly Tyr Met Ala Pro Thr Met Arg Gln Gly Gln Ala Tyr 3895 3900 3905 cca gct aac tac ccc tac cca ctt att gga acc act gct gtt aaa 21134 Pro Ala Asn Tyr Pro Tyr Pro Leu Ile Gly Thr Thr Ala Val Lys 3910 3915 3920 agc gtc acc cag aaa aag ttc ctg tgt gac agg acc atg tgg cgc 21179 Ser Val Thr Gln Lys Lys Phe Leu Cys Asp Arg Thr Met Trp Arg 3925 3930 3935 atc ccc ttc tcc agc aac ttc atg tcc atg ggt gcc ctt acc gac 21224 Ile Pro Phe Ser Ser Asn Phe Met Ser Met Gly Ala Leu Thr Asp 3940 3945 3950 ctg gga cag aac atg ctt tat gcc aac tca gcc cat gcg ctg gat 21269 Leu Gly Gln Asn Met Leu Tyr Ala Asn Ser Ala His Ala Leu Asp 3955 3960 3965 atg act ttt gag gtg gat ccc atg gat gag ccc acc ctg ctt tat 21314 Met Thr Phe Glu Val Asp Pro Met Asp Glu Pro Thr Leu Leu Tyr 3970 3975 3980 ctt ctt ttc gaa gtt ttc gac gtg gtc aga gtg cac cag cca cac 21359 Leu Leu Phe Glu Val Phe Asp Val Val Arg Val His Gln Pro His 3985 3990 3995 cgc ggc gtc atc gag gct gtc tac ctg cgt acc cca ttc tca gct 21404 Arg Gly Val Ile Glu Ala Val Tyr Leu Arg Thr Pro Phe Ser Ala 4000 4005 4010 ggt aac gcc act aca taagcccctt gcttcttgca agcagtagct gcagc atg 21457 Gly Asn Ala Thr Thr Met 4015 gcc tgc ggg tcc ggc aac gga tcc agt gag caa gag ctc agg gcc 21502 Ala Cys Gly Ser Gly Asn Gly Ser Ser Glu Gln Glu Leu Arg Ala 4020 4025 4030 atc gct aga gat ttg ggc tgc gga ccc tat ttc ctg gga act ttt 21547 Ile Ala Arg Asp Leu Gly Cys Gly Pro Tyr Phe Leu Gly Thr Phe 4035 4040 4045 gac aag cga ttc ccg ggg ttc atg gct ccc gac aag ctc gcc tgt 21592 Asp Lys Arg Phe Pro Gly Phe Met Ala Pro Asp Lys Leu Ala Cys 4050 4055 4060 gcc att gtc aat acg gcc ggt cgc gag acg ggg ggt gaa cat tgg 21637 Ala Ile Val Asn Thr Ala Gly Arg Glu Thr Gly Gly Glu His Trp 4065 4070 4075 ctg gct ttt ggt tgg aat ccg cgc tcc aac acc tgc tac ctt ttt 21682 Leu Ala Phe Gly Trp Asn Pro Arg Ser Asn Thr Cys Tyr Leu Phe 4080 4085 4090 gat cct ttt ggc ttc tcg gac gag cgc ctt aag caa atc tac cag 21727 Asp Pro Phe Gly Phe Ser Asp Glu Arg Leu Lys Gln Ile Tyr Gln 4095 4100 4105 ttc gaa tat gag ggg ctc ctg cgc cgc agc gcc ctt gct acc aag 21772 Phe Glu Tyr Glu Gly Leu Leu Arg Arg Ser Ala Leu Ala Thr Lys 4110 4115 4120 gac cgc tgt atc acc ctc gaa aag tca acc cag acc gtg cag ggt 21817 Asp Arg Cys Ile Thr Leu Glu Lys Ser Thr Gln Thr Val Gln Gly 4125 4130 4135 ccg cgc tcc gca gcc tgt gga ctt ttt tgc tgc atg ttc ctc cac 21862 Pro Arg Ser Ala Ala Cys Gly Leu Phe Cys Cys Met Phe Leu His 4140 4145 4150 gct ttt gtg cac tgg ccc gac cgc ccc atg gac gga aac ccc acc 21907 Ala Phe Val His Trp Pro Asp Arg Pro Met Asp Gly Asn Pro Thr 4155 4160 4165 atg aag ttg cta act ggg gta ccc aac agc atg ctt caa tca ccc 21952 Met Lys Leu Leu Thr Gly Val Pro Asn Ser Met Leu Gln Ser Pro 4170 4175 4180 caa gtc cag ccc acc ctg cgc cac aac cag gag gcg ctc tac cgc 21997 Gln Val Gln Pro Thr Leu Arg His Asn Gln Glu Ala Leu Tyr Arg 4185 4190 4195 ttc ctc aac acc cac tca tct tac ttt cgt tct cac cgc gcg cgc 22042 Phe Leu Asn Thr His Ser Ser Tyr Phe Arg Ser His Arg Ala Arg 4200 4205 4210 atc gaa aag gct acc gcg ttt gac cgt atg gat atg caa taataagtca 22091 Ile Glu Lys Ala Thr Ala Phe Asp Arg Met Asp Met Gln 4215 4220 4225 tgtaaaaccg tgttcaaata aacagcactt tattttttac atgcactgtg gctctgggtt 22151 gctcattcat tcatcattcg ctcagaagtc gaaggggttc tggcgggaat cagcgtgacc 22211 cgcgggcagg gatacgttgc ggaactggaa cctgttctgc cacttgaact cggggatcac 22271 cagcttggga actggaatct cggggaaggt gtcttgccac agctttctgg tcagttgcag 22331 agcgccgagc aggtcaggag cagagatctt gaaatcacag ttggggccag cattctgggc 22391 acgggagttg cggtacactg ggttgcagca ctggaacacc atcagggcgg ggtgtctcac 22451 gctcgccagc acggtcgggt cgctgatggt agtcacatcc aagtcttcag cattggccat 22511 tccaaagggg gtcatcttac aggtctgcct gcccatcacg ggagcgcagc cgggcttgtg 22571 gttgcaatcg cagcgaatgg gtatcagcat catcctggcc tggtcggggg ttatccctgg 22631 atacaccgcc ttcataaagg cttcgtactg cttgaaagct tcctgggcct tgcttccctc 22691 ggtgtagaac atcccacagg acttgctgga aaattgatta gtagcacagt tggcatcatt 22751 cacacagcag cgggcatcgt tgttggccag ctggaccaca ttcctgcccc agcggttctg 22811 ggtgatcttg gctcggtctg ggttctcctt catcgcgcgc tgcccgttct cgctcgccac 22871 atccatctcg atgatgtgat ccttctggat catgatagtg ccatgcaggc atttcacctt 22931 gccttcataa tcggtgcagc catgagccca tagagcgcac ccggtgcact cccaattgtt 22991 gtgggcgatc tccgaatacg aatgcaccaa tccctgcagg aatcttccca tcattgcagt 23051 cagggtcttc aagctggtaa atgtcagcgg gatgccacgg tgctcctcgt tcacatactg 23111 gtggcagata cgcctgtact gctcgtgctg ctctggcatc agcttgaaag aggttctcag 23171 gtcattatcc agcctgtacc tctccatcag tacggccatt acttccatgc ccttctccca 23231 ggcagagacc aagggcaggc tcatgggatt cctaacagca atagcagcag atgctccttt 23291 agccagaggg tcattcttgt caatcttctc gacacttctc ttgccatcct tctcagtgat 23351 gcgcacgggt gggtagctga agcccacggc caccagctcc gcctgttctc tttcttcttc 23411 gctgtcctgg ctgatgtctt gcagagggac atgcttggtc tttctgggtt tcttcttggg 23471 agggatcggg ggagggctgt tgctccgctc tgaagacagg gaggaccgcg aagtttcgct 23531 caccagcacc acctggctct cggtagaaga acctgacccc acacggcggt aggtgttcct 23591 cttcgggggc agaggtggag gcgactgcga tgggctgcgg tccggcctgg gaggcggatg 23651 gctggcagag cctcttccgc gttcgggggt gtgctcccgg tggcggtcgc ttgactgatt 23711 tcctccgcgg ctggccattg tgttctccta ggcagagaaa caacagac atg gag act 23768 Met Glu Thr cag cca tcg ctg cca aca ccg ctg caa gcg cca tca cac ctc gcc 23813 Gln Pro Ser Leu Pro Thr Pro Leu Gln Ala Pro Ser His Leu Ala 4230 4235 4240 ccc agc agc gac gag gag gaa cag agc tta acc acc cca cca ccc 23858 Pro Ser Ser Asp Glu Glu Glu Gln Ser Leu Thr Thr Pro Pro Pro 4245 4250 4255 agt ccc gcc acc acc acc tct acc ctc gat gag gag gag gag gtc 23903 Ser Pro Ala Thr Thr Thr Ser Thr Leu Asp Glu Glu Glu Glu Val 4260 4265 4270 gac gca ccc cag gag atg cag gtt atg gag gat gag aaa gcg gaa 23948 Asp Ala Pro Gln Glu Met Gln Val Met Glu Asp Glu Lys Ala Glu 4275 4280 4285 gag att gag gca gat gtc gag cag gac ccg ggc tat gtg aca ccg 23993 Glu Ile Glu Ala Asp Val Glu Gln Asp Pro Gly Tyr Val Thr Pro 4290 4295 4300 gcg gag cac gag gag gag ctg aga cgc ttt cta gac aga gag gat 24038 Ala Glu His Glu Glu Glu Leu Arg Arg Phe Leu Asp Arg Glu Asp 4305 4310 4315 aac aac cgc cca gag cag caa gca gat ggc gat cac cag gag gct 24083 Asn Asn Arg Pro Glu Gln Gln Ala Asp Gly Asp His Gln Glu Ala 4320 4325 4330 ggg ctc ggg gat cat gtc gcc gaa tac ctc acc ggg ctt ggc ggg 24128 Gly Leu Gly Asp His Val Ala Glu Tyr Leu Thr Gly Leu Gly Gly 4335 4340 4345 gag gac gtg ctc ctc aaa cat cta gca agg cag acg atc ata gtc 24173 Glu Asp Val Leu Leu Lys His Leu Ala Arg Gln Thr Ile Ile Val 4350 4355 4360 aaa gac gca ctg ctc gac cgc acc gaa gtg ccc atc agt gtg gaa 24218 Lys Asp Ala Leu Leu Asp Arg Thr Glu Val Pro Ile Ser Val Glu 4365 4370 4375 gag ctc agc cgc gcc tac gag ctc aac ctg ttc tcg cct cgg gtg 24263 Glu Leu Ser Arg Ala Tyr Glu Leu Asn Leu Phe Ser Pro Arg Val 4380 4385 4390 ccc ccc aag cgt cag caa aac ggc acc tgc gag ccc aac cct cgc 24308 Pro Pro Lys Arg Gln Gln Asn Gly Thr Cys Glu Pro Asn Pro Arg 4395 4400 4405 ctc aac ttc tat ccg gcc ttt gct gtc cca gaa gtg ctt gct acc 24353 Leu Asn Phe Tyr Pro Ala Phe Ala Val Pro Glu Val Leu Ala Thr 4410 4415 4420 tac cac atc ttt ttc aag aac caa aag att cca gtc tcc tgc cgt 24398 Tyr His Ile Phe Phe Lys Asn Gln Lys Ile Pro Val Ser Cys Arg 4425 4430 4435 gcc aac cgc acc cgc gcc gat gcc ctg ctc aac ttg gga cct ggc 24443 Ala Asn Arg Thr Arg Ala Asp Ala Leu Leu Asn Leu Gly Pro Gly 4440 4445 4450 gct cgc tta cct gat ata gct tcc ttg gaa gag gtt cca aag atc 24488 Ala Arg Leu Pro Asp Ile Ala Ser Leu Glu Glu Val Pro Lys Ile 4455 4460 4465 ttc gag ggt ctg ggc agt gat gag act cgg gcc gca aat gct ctg 24533 Phe Glu Gly Leu Gly Ser Asp Glu Thr Arg Ala Ala Asn Ala Leu 4470 4475 4480 caa cag gga gaa aat ggc atg gat gaa cat cac agc gct ctg gtg 24578 Gln Gln Gly Glu Asn Gly Met Asp Glu His His Ser Ala Leu Val 4485 4490 4495 gag ttg gag gga gac aat gcc cgg ctt gca gtg ctc aag cgc agt 24623 Glu Leu Glu Gly Asp Asn Ala Arg Leu Ala Val Leu Lys Arg Ser 4500 4505 4510 atc gag gtc acc cat ttt gcc tac ccc gct gtc aac ctg ccc ccc 24668 Ile Glu Val Thr His Phe Ala Tyr Pro Ala Val Asn Leu Pro Pro 4515 4520 4525 aaa gtc atg agc gct gtc atg gac cag ctg ctc atc aag cga gca 24713 Lys Val Met Ser Ala Val Met Asp Gln Leu Leu Ile Lys Arg Ala 4530 4535 4540 agc ccc ctt tcc gaa gac cag aac atg cag gat cca gac gcc tct 24758 Ser Pro Leu Ser Glu Asp Gln Asn Met Gln Asp Pro Asp Ala Ser 4545 4550 4555 gac gag ggc aag ccg gtg gtc agt gac gag cag ctg tct cgc tgg 24803 Asp Glu Gly Lys Pro Val Val Ser Asp Glu Gln Leu Ser Arg Trp 4560 4565 4570 ctg agt act aac tcc cca cga gac ttg gaa gag agg cgc aag ctt 24848 Leu Ser Thr Asn Ser Pro Arg Asp Leu Glu Glu Arg Arg Lys Leu 4575 4580 4585 atg atg gct gta gtg cta gtc act gtg gag ctg gag tgt ctc cgc 24893 Met Met Ala Val Val Leu Val Thr Val Glu Leu Glu Cys Leu Arg 4590 4595 4600 cgc ttt ttc acc gac cct gag acc ctg cgc aag ctc gag gag aac 24938 Arg Phe Phe Thr Asp Pro Glu Thr Leu Arg Lys Leu Glu Glu Asn 4605 4610 4615 ctg cac tat act ttc aga cat ggc ttc gtg cgc cag gca tgc aag 24983 Leu His Tyr Thr Phe Arg His Gly Phe Val Arg Gln Ala Cys Lys 4620 4625 4630 atc tcc aac gtg gag ctc acc aac ctg gtc tcc tac atg ggc att 25028 Ile Ser Asn Val Glu Leu Thr Asn Leu Val Ser Tyr Met Gly Ile 4635 4640 4645 ttg cat gag aac cgc ctg ggg cag agc gtg ttg cat acc acc ctg 25073 Leu His Glu Asn Arg Leu Gly Gln Ser Val Leu His Thr Thr Leu 4650 4655 4660 aaa ggg gag gcc cgc cgc gac tac atc cgc gac tgt gtc tac ctc 25118 Lys Gly Glu Ala Arg Arg Asp Tyr Ile Arg Asp Cys Val Tyr Leu 4665 4670 4675 tac ctc tgc cat acc tgg cag act ggc atg ggt gta tgg cag cag 25163 Tyr Leu Cys His Thr Trp Gln Thr Gly Met Gly Val Trp Gln Gln 4680 4685 4690 tgt ttg gaa gag cag aac ctg aaa gag ctg gac aag ctc ttg cag 25208 Cys Leu Glu Glu Gln Asn Leu Lys Glu Leu Asp Lys Leu Leu Gln 4695 4700 4705 aga tcc ctt aaa gcc ctg tgg aca ggt ttt gac gag cgc acc gtc 25253 Arg Ser Leu Lys Ala Leu Trp Thr Gly Phe Asp Glu Arg Thr Val 4710 4715 4720 gcc tca gac ctg gca gac atc atc ttc ccc gag cgt ctc agg gtt 25298 Ala Ser Asp Leu Ala Asp Ile Ile Phe Pro Glu Arg Leu Arg Val 4725 4730 4735 act ctg cgc aac ggc ctg cct gac ttc atg agc caa agc atg ctt 25343 Thr Leu Arg Asn Gly Leu Pro Asp Phe Met Ser Gln Ser Met Leu 4740 4745 4750 aac aac ttt cgc tct ttc atc ctg gaa cgc tcc ggt atc ctg ccc 25388 Asn Asn Phe Arg Ser Phe Ile Leu Glu Arg Ser Gly Ile Leu Pro 4755 4760 4765 gcc acc tgc tgc gcg ctg ccc tcc gac ttt gtg cct ctc acc tac 25433 Ala Thr Cys Cys Ala Leu Pro Ser Asp Phe Val Pro Leu Thr Tyr 4770 4775 4780 cgc gag tgc ccc ccg ccg cta tgg agc cac tgc tac ctg ttc cgc 25478 Arg Glu Cys Pro Pro Pro Leu Trp Ser His Cys Tyr Leu Phe Arg 4785 4790 4795 ctg gcc aac tac ctt tcc tac cac tcg gat gtg atc gag gat gtg 25523 Leu Ala Asn Tyr Leu Ser Tyr His Ser Asp Val Ile Glu Asp Val 4800 4805 4810 agc gga gac ggc ctg ctg gag tgc cac tgc cgc tgc aat ctc tgc 25568 Ser Gly Asp Gly Leu Leu Glu Cys His Cys Arg Cys Asn Leu Cys 4815 4820 4825 aca ccc cac cgt tcc ctc gcc tgc aac ccc cag ttg ctg agc gag 25613 Thr Pro His Arg Ser Leu Ala Cys Asn Pro Gln Leu Leu Ser Glu 4830 4835 4840 acc cag atc atc ggc acc ttc gag ttg cag ggt ccc agc agc gaa 25658 Thr Gln Ile Ile Gly Thr Phe Glu Leu Gln Gly Pro Ser Ser Glu 4845 4850 4855 ggc gag ggg tct tct tcg ggg cag agt ctg aaa ctg acc ccg ggg 25703 Gly Glu Gly Ser Ser Ser Gly Gln Ser Leu Lys Leu Thr Pro Gly 4860 4865 4870 cta tgg acc tcc gcc tac ctg cgc aag ttc gcc ccc gaa gac tac 25748 Leu Trp Thr Ser Ala Tyr Leu Arg Lys Phe Ala Pro Glu Asp Tyr 4875 4880 4885 cac ccc tat gag att agg ttc tat gag gac caa tca cag ccg ccc 25793 His Pro Tyr Glu Ile Arg Phe Tyr Glu Asp Gln Ser Gln Pro Pro 4890 4895 4900 aaa gcc gag ctc tca gcc tgc gtc atc act cag ggg gca att ctc 25838 Lys Ala Glu Leu Ser Ala Cys Val Ile Thr Gln Gly Ala Ile Leu 4905 4910 4915 gcc caa ttg caa gcc atc caa aaa tcc cgc caa gaa ttt ctg ctg 25883 Ala Gln Leu Gln Ala Ile Gln Lys Ser Arg Gln Glu Phe Leu Leu 4920 4925 4930 aaa agg ggg aac ggg gtc tac ctc gac ccc cag acc ggt gag gag 25928 Lys Arg Gly Asn Gly Val Tyr Leu Asp Pro Gln Thr Gly Glu Glu 4935 4940 4945 ctc aac aca agg ttc cct cag gat gtc cca gcg ccg agg aag caa 25973 Leu Asn Thr Arg Phe Pro Gln Asp Val Pro Ala Pro Arg Lys Gln 4950 4955 4960 gaa gtt gaa ggt gca gct gcc gcc ccc aga gga tat gga gga aga 26018 Glu Val Glu Gly Ala Ala Ala Ala Pro Arg Gly Tyr Gly Gly Arg 4965 4970 4975 ctg gga cag tca ggc aga gga gga gga gat gga aga ttg gga cag 26063 Leu Gly Gln Ser Gly Arg Gly Gly Gly Asp Gly Arg Leu Gly Gln 4980 4985 4990 cca ggc aga gga ggc gga cag cct gga gga aga cag ttt gga gga 26108 Pro Gly Arg Gly Gly Gly Gln Pro Gly Gly Arg Gln Phe Gly Gly 4995 5000 5005 gga aga cga gga ggc aga gga ggt gga aga agc aac cgc cgc caa 26153 Gly Arg Arg Gly Gly Arg Gly Gly Gly Arg Ser Asn Arg Arg Gln 5010 5015 5020 aca gtt gtc ctc gac agc gga gac aag cag ggc ccc aga cag cag 26198 Thr Val Val Leu Asp Ser Gly Asp Lys Gln Gly Pro Arg Gln Gln 5025 5030 5035 cag cag cac ggc tac aat ctc cgc tcc ggg tcg ggg ggc cca gcg 26243 Gln Gln His Gly Tyr Asn Leu Arg Ser Gly Ser Gly Gly Pro Ala 5040 5045 5050 gcg tcc caa cag tagatgggac gagaccgggc gattcccgaa cccgaccacc 26295 Ala Ser Gln Gln 5055 gcttccaaga ccggtaagaa ggagcggcag ggatacaagt cctggcgggg gcataagaat 26355 gccatcatct cctgcttgca tgaatgtggg ggcaacatat ccttcacccg gcgctacctg 26415 ctcttccacc acggggtgaa cttcccccgc aatgtcttgc attactaccg tcacctccac 26475 agcccctact acagccagca agtcccaaca gcctcggcag agaaaaacag cagcagcggg 26535 gacctccagc agaaaaccag cagcagcagt tagaaagtcc agtgcagcag gaggaggact 26595 gaggatcaca gcgaacgagc cagcgcagac ccgagagctg agaaacagga tctttccaac 26655 cctctatgcc atcttccagc agagtcgggg gcaagagcag gaactgaaag taaaaaaccg 26715 atctctgcgc tcgctcaccc gaagttgttt gtatcacaag agcgaagacc aacttcagcg 26775 cactctcgag gacgccgagg ctctcttcaa caagtactgc gcgctgactc ttaaagagta 26835 gcccgcgccc gcgctcgctc gaaaaaggcg ggaattacgt cacccttggc acctgtcctt 26895 tgccccgtc atg agt aaa gaa att ccc acg cct tac atg tgg agc tat 26943 Met Ser Lys Glu Ile Pro Thr Pro Tyr Met Trp Ser Tyr 5060 5065 5070 cag ccc caa atg gga ctg gca gca ggc gcc tcc cag gac tac tcc 26988 Gln Pro Gln Met Gly Leu Ala Ala Gly Ala Ser Gln Asp Tyr Ser 5075 5080 5085 acc cgc atg aat tgg ctc agc gcc ggg ccc tcg atg atc tca cgg 27033 Thr Arg Met Asn Trp Leu Ser Ala Gly Pro Ser Met Ile Ser Arg 5090 5095 5100 gtt aat gat ata cga gct tac cga aac cag tta ctc cta gaa cag 27078 Val Asn Asp Ile Arg Ala Tyr Arg Asn Gln Leu Leu Leu Glu Gln 5105 5110 5115 tca gct ctc acc acc aca ccc cgc caa cac ctt aat ccc cgg aat 27123 Ser Ala Leu Thr Thr Thr Pro Arg Gln His Leu Asn Pro Arg Asn 5120 5125 5130 tgg ccc gcc gcc ctg gtg tac cag gaa acc ccc gct ccc acc acc 27168 Trp Pro Ala Ala Leu Val Tyr Gln Glu Thr Pro Ala Pro Thr Thr 5135 5140 5145 gta cta ctt cct cga gac gcc cag gcc gaa gtt cag atg act aac 27213 Val Leu Leu Pro Arg Asp Ala Gln Ala Glu Val Gln Met Thr Asn 5150 5155 5160 gca ggt gta cag ctg gcg ggc ggt tcc gcc ctg tgt cgt cac cgg 27258 Ala Gly Val Gln Leu Ala Gly Gly Ser Ala Leu Cys Arg His Arg 5165 5170 5175 cct cag cag agt ata aaa cgc ctg gtg atc aga ggc cga ggt atc 27303 Pro Gln Gln Ser Ile Lys Arg Leu Val Ile Arg Gly Arg Gly Ile 5180 5185 5190 cag ctc aac gac gag tcg gtg agc tct tcg ctt ggt ctg cga cca 27348 Gln Leu Asn Asp Glu Ser Val Ser Ser Ser Leu Gly Leu Arg Pro 5195 5200 5205 gac gga gtc ttc caa atc gcc ggc tgt ggg aga tct tcc ttc act 27393 Asp Gly Val Phe Gln Ile Ala Gly Cys Gly Arg Ser Ser Phe Thr 5210 5215 5220 cct cgt cag gct gtc ctg act ttg gag agt tcg tcc tcg cag ccc 27438 Pro Arg Gln Ala Val Leu Thr Leu Glu Ser Ser Ser Ser Gln Pro 5225 5230 5235 cgc tcg ggc ggc atc ggg act ctc cag ttt gtg gag gag ttt act 27483 Arg Ser Gly Gly Ile Gly Thr Leu Gln Phe Val Glu Glu Phe Thr 5240 5245 5250 ccc tct gtc tac ttc aac ccc ttc tcc ggc tct cct ggc cag tac 27528 Pro Ser Val Tyr Phe Asn Pro Phe Ser Gly Ser Pro Gly Gln Tyr 5255 5260 5265 ccg gac gag ttc ata ccg aac ttc gac gca atc agc gag tca gtg 27573 Pro Asp Glu Phe Ile Pro Asn Phe Asp Ala Ile Ser Glu Ser Val 5270 5275 5280 gat ggc tat gat tg atg tct ggt ggc gcg gct gag tta gct cga ctg 27620 Asp Gly Tyr Asp Met Ser Gly Gly Ala Ala Glu Leu Ala Arg Leu 5285 5290 5295 cga cat cta gac cac tgc cgc cgc ttt cgc tgt ttc gcc cgg gaa 27665 Arg His Leu Asp His Cys Arg Arg Phe Arg Cys Phe Ala Arg Glu 5300 5305 5310 ctc acc gag ttc atc tac ttc gaa ctc ccc gag gag cac cct cag 27710 Leu Thr Glu Phe Ile Tyr Phe Glu Leu Pro Glu Glu His Pro Gln 5315 5320 5325 gga ccg gcc cac gga gtg cgg att acc atc gaa ggg gga ata gac 27755 Gly Pro Ala His Gly Val Arg Ile Thr Ile Glu Gly Gly Ile Asp 5330 5335 5340 tct cgc ctg cat cgg atc ttc tgc cag cga cct gtg ctg att gag 27800 Ser Arg Leu His Arg Ile Phe Cys Gln Arg Pro Val Leu Ile Glu 5345 5350 5355 cgc gac cag gga act aca aca gtc tcc atc tac tgc atc tgt aac 27845 Arg Asp Gln Gly Thr Thr Thr Val Ser Ile Tyr Cys Ile Cys Asn 5360 5365 5370 cac ccc gga ttg cat gaa agc ctt tgc tgt ctt att tgt gct gag 27890 His Pro Gly Leu His Glu Ser Leu Cys Cys Leu Ile Cys Ala Glu 5375 5380 5385 ttt aat aaa aac tgagttaaga ctcaccttcg gactaccgct tcttcaaccc 27942 Phe Asn Lys Asn ggaccttaca acaccagcca gaccctccgt tccagccaga agaaccagac ccttcctcta 28002 atccaggact ctaattctac ctccccagcg ccttttccta ctaaccttcc cgatactaac 28062 aacctcggag ctcagctgca acaccgcttc tccagaagcc tcctttctgc caatactact 28122 actcccaaaa ccggaggtga gctccgtggt ctccctactg acaacccctg ggtggtagcg 28182 ggttttgtag cactaggagt agttgcgggt gggctggtgc ttatcctctg ctacctatac 28242 acaccttgct gtgcttattt agtagtcttg tgctgttggt ttaagaa atg ggg gtc 28298 Met Gly Val 5390 gta cta gtc gcg ctt gct tta ctt tcg ctt ttg ggt ctg ggc tct 28343 Val Leu Val Ala Leu Ala Leu Leu Ser Leu Leu Gly Leu Gly Ser 5395 5400 5405 act acg cta gcg aat cag cct tta cta tta gat cct gat aat gtt 28388 Thr Thr Leu Ala Asn Gln Pro Leu Leu Leu Asp Pro Asp Asn Val 5410 5415 5420 gat cca tgc cta aca ttt gat cca gaa aac tgc aca ctt act ttt 28433 Asp Pro Cys Leu Thr Phe Asp Pro Glu Asn Cys Thr Leu Thr Phe 5425 5430 5435 gca cct gaa aca agt cgc tac tgt gga gtt ctt att agg tgc gga 28478 Ala Pro Glu Thr Ser Arg Tyr Cys Gly Val Leu Ile Arg Cys Gly 5440 5445 5450 cgg gaa tgc agg ccc att gag att aca cac aat aac aaa act tgg 28523 Arg Glu Cys Arg Pro Ile Glu Ile Thr His Asn Asn Lys Thr Trp 5455 5460 5465 aac aac aca tta ttc acc aca tgg tct cca gga gat cct cag tgg 28568 Asn Asn Thr Leu Phe Thr Thr Trp Ser Pro Gly Asp Pro Gln Trp 5470 5475 5480 tat act gtc tct gtc cgg ggt cct gac ggt tcc gtc cgc ata gct 28613 Tyr Thr Val Ser Val Arg Gly Pro Asp Gly Ser Val Arg Ile Ala 5485 5490 5495 aat aac act ttc att ttt gct gaa atg tgc gat atg gtc atg ttc 28658 Asn Asn Thr Phe Ile Phe Ala Glu Met Cys Asp Met Val Met Phe 5500 5505 5510 atg agc aga cag tat aac cta tgg cct ccc agc gag gaa aac att 28703 Met Ser Arg Gln Tyr Asn Leu Trp Pro Pro Ser Glu Glu Asn Ile 5515 5520 5525 gtg gca ttc tcc att gct tat tgc tta tgt act tgc ctt atc act 28748 Val Ala Phe Ser Ile Ala Tyr Cys Leu Cys Thr Cys Leu Ile Thr 5530 5535 5540 gct atc ttg tgt gcg tgc ttg cat ttg ctt att gct att cgc tcc 28793 Ala Ile Leu Cys Ala Cys Leu His Leu Leu Ile Ala Ile Arg Ser 5545 5550 5555 aga aac aat gag gaa aaa gaa aaa atg cct taaccttttt cctcatacct 28843 Arg Asn Asn Glu Glu Lys Glu Lys Met Pro 5560 5565 tttttacagc atg act tct gtc gca gtc att ttt act att att acc ggc 28892 Met Thr Ser Val Ala Val Ile Phe Thr Ile Ile Thr Gly 5570 5575 5580 ttt act act gcc gtg cat gga atg aaa aat gtt aaa cta act gtc 28937 Phe Thr Thr Ala Val His Gly Met Lys Asn Val Lys Leu Thr Val 5585 5590 5595 tat act aac acc aac caa aca ctg gag ggg cct aaa ggg aca gtt 28982 Tyr Thr Asn Thr Asn Gln Thr Leu Glu Gly Pro Lys Gly Thr Val 5600 5605 5610 tca tgg tat tgg tat caa aat ttt ggc gat ctg tct gta tgg ttg 29027 Ser Trp Tyr Trp Tyr Gln Asn Phe Gly Asp Leu Ser Val Trp Leu 5615 5620 5625 tgc gat gga aca acc att aat aag acc att gat tta att aaa tac 29072 Cys Asp Gly Thr Thr Ile Asn Lys Thr Ile Asp Leu Ile Lys Tyr 5630 5635 5640 agt tgc gat tca gat tta aca cta atc aac att aat gct cat tat 29117 Ser Cys Asp Ser Asp Leu Thr Leu Ile Asn Ile Asn Ala His Tyr 5645 5650 5655 gaa ggt tac tat tat gga act gac ata aat gat gta aac ttc tac 29162 Glu Gly Tyr Tyr Tyr Gly Thr Asp Ile Asn Asp Val Asn Phe Tyr 5660 5665 5670 aac att tat gta tca gac cca aca acc att cca act aaa ccc tcc 29207 Asn Ile Tyr Val Ser Asp Pro Thr Thr Ile Pro Thr Lys Pro Ser 5675 5680 5685 aca cac act aaa act tac act aaa act tcc aca cac aca agc atc 29252 Thr His Thr Lys Thr Tyr Thr Lys Thr Ser Thr His Thr Ser Ile 5690 5695 5700 aat gag tta caa ttt cta aaa gct aac ata aca tac aat tct acc 29297 Asn Glu Leu Gln Phe Leu Lys Ala Asn Ile Thr Tyr Asn Ser Thr 5705 5710 5715 atc tcg cct act att ccc aat gaa aca aat att cct aat tca atg 29342 Ile Ser Pro Thr Ile Pro Asn Glu Thr Asn Ile Pro Asn Ser Met 5720 5725 5730 att gga att att gct gca gtt gct att gga atg gcg atc ata ata 29387 Ile Gly Ile Ile Ala Ala Val Ala Ile Gly Met Ala Ile Ile Ile 5735 5740 5745 ata tgt atg atc gtt tat gct tgc tgc tat aaa aaa ctt caa gaa 29432 Ile Cys Met Ile Val Tyr Ala Cys Cys Tyr Lys Lys Leu Gln Glu 5750 5755 5760 gaa aaa tta gat cca cta cta agc ttt gat ttt taaatttttt 29475 Glu Lys Leu Asp Pro Leu Leu Ser Phe Asp Phe 5765 5770 tttgtagaaa c atg ctt ctt cat act tta gct ttt att tcc att ttt 29522 Met Leu Leu His Thr Leu Ala Phe Ile Ser Ile Phe 5775 5780 ggt ttc tca ctt gga ggt aaa ata cat aag aat gtt acc gtg tta 29567 Gly Phe Ser Leu Gly Gly Lys Ile His Lys Asn Val Thr Val Leu 5785 5790 5795 gag ggc gct cca aat ata aca ctc caa gga gtt tat gtc cca ccc 29612 Glu Gly Ala Pro Asn Ile Thr Leu Gln Gly Val Tyr Val Pro Pro 5800 5805 5810 agt caa aaa aga agc act att aac ata act tgg gaa act gta ata 29657 Ser Gln Lys Arg Ser Thr Ile Asn Ile Thr Trp Glu Thr Val Ile 5815 5820 5825 aat gga agc aga cca aat gta tgt gct tta aat tta aca aaa ttc 29702 Asn Gly Ser Arg Pro Asn Val Cys Ala Leu Asn Leu Thr Lys Phe 5830 5835 5840 aaa tgt gag ggt ttt gat ctt act atc ttt aat tta act aaa caa 29747 Lys Cys Glu Gly Phe Asp Leu Thr Ile Phe Asn Leu Thr Lys Gln 5845 5850 5855 gac tcc aaa aat tat ttt ggt gaa agt ata act gtc tta act tct 29792 Asp Ser Lys Asn Tyr Phe Gly Glu Ser Ile Thr Val Leu Thr Ser 5860 5865 5870 ggt tat caa aaa aat tat ata acc cac aat tat gca gtt tat cat 29837 Gly Tyr Gln Lys Asn Tyr Ile Thr His Asn Tyr Ala Val Tyr His 5875 5880 5885 gtt att gtt ata tct cca act act cat gcg ccc tct acc aca caa 29882 Val Ile Val Ile Ser Pro Thr Thr His Ala Pro Ser Thr Thr Gln 5890 5895 5900 gta act aca gct cat tct aac act tac acc cat gta aag gta tta 29927 Val Thr Thr Ala His Ser Asn Thr Tyr Thr His Val Lys Val Leu 5905 5910 5915 aaa gaa aca tac agt act acc aat gtg cag act act ata atc aca 29972 Lys Glu Thr Tyr Ser Thr Thr Asn Val Gln Thr Thr Ile Ile Thr 5920 5925 5930 aaa ata ccc aca aca gct acc tca ttt gct tta cac aaa tca gca 30017 Lys Ile Pro Thr Thr Ala Thr Ser Phe Ala Leu His Lys Ser Ala 5935 5940 5945 ctt tca tgc gcc cca acg ctc acc act ttg cat gct act aaa cct 30062 Leu Ser Cys Ala Pro Thr Leu Thr Thr Leu His Ala Thr Lys Pro 5950 5955 5960 ttt act aat ata tct act ccc tta aaa caa ttt gat cga act atg 30107 Phe Thr Asn Ile Ser Thr Pro Leu Lys Gln Phe Asp Arg Thr Met 5965 5970 5975 aaa ata gaa att acc ttt ctt att gtc ata gga ata att atc att 30152 Lys Ile Glu Ile Thr Phe Leu Ile Val Ile Gly Ile Ile Ile Ile 5980 5985 5990 gca atc ttg ctt tac tac ata ttc tgt cgc caa atc ccc aat gct 30197 Ala Ile Leu Leu Tyr Tyr Ile Phe Cys Arg Gln Ile Pro Asn Ala 5995 6000 6005 caa aga cga cct ata tat aga cct atc ata ggt gaa ccc caa caa 30242 Gln Arg Arg Pro Ile Tyr Arg Pro Ile Ile Gly Glu Pro Gln Gln 6010 6015 6020 ctt caa gtg gag gga ggc tta aga aat ctt ctg ttc tct ttt aca 30287 Leu Gln Val Glu Gly Gly Leu Arg Asn Leu Leu Phe Ser Phe Thr 6025 6030 6035 gta tgg tgatcaacaa tc atg atc cct aga aat ttc ctc ttc acc ata 30335 Val Trp Met Ile Pro Arg Asn Phe Leu Phe Thr Ile 6040 6045 6050 ctc atc tgt gct ttt aat gtt tgt gct act ttc gcc aca gtt gcc 30380 Leu Ile Cys Ala Phe Asn Val Cys Ala Thr Phe Ala Thr Val Ala 6055 6060 6065 aat gtc act cca gac tgt ata gga gca ttt gcc tcc tat gtg ctt 30425 Asn Val Thr Pro Asp Cys Ile Gly Ala Phe Ala Ser Tyr Val Leu 6070 6075 6080 ttc gca ttt att acc tgc atc tgt gtt tgt agc ata gtt tgc ctg 30470 Phe Ala Phe Ile Thr Cys Ile Cys Val Cys Ser Ile Val Cys Leu 6085 6090 6095 gtt att aat ttc ttt caa ctt gta gac tgg gtt ttt gta cgc gtt 30515 Val Ile Asn Phe Phe Gln Leu Val Asp Trp Val Phe Val Arg Val 6100 6105 6110 gcc tac ctg cgg cat cac cct gaa tac cgc aac caa aat gtt gca 30560 Ala Tyr Leu Arg His His Pro Glu Tyr Arg Asn Gln Asn Val Ala 6115 6120 6125 gca att ctt agg ctc att taaaaccatg caaactctgc tactacttct 30608 Ala Ile Leu Arg Leu Ile 6130 gctagttata catccatgtg cctccttaaa ccccacaagc cccacaaaat tacacctaag 30668 aaaatgtaaa tttcaagaac catggaaatt ccttgaatgc tatcatgaaa catctgattt 30728 ccccacatac tggattacaa tcattgggat tgttaatcta gtttcttgca cactattctc 30788 tttccttgtt taccacttat ttgattttgg atggaatgcc ctcaatgcac tcacttaccc 30848 acaagaacca gaggaacata taccactaca aaacatgcaa ccactagcac tagtagaata 30908 tgaaaatgaa ccacagcccc cgatgctccc tgctattagt tacttcaacc taactggagg 30968 ag atg act gac cca ctc gcc gcc tcc gct gct gag gaa cta ctt gat 31015 Met Thr Asp Pro Leu Ala Ala Ser Ala Ala Glu Glu Leu Leu Asp 6135 6140 6145 atg gat ggc cgt gcc tcc gaa cag cga ctc gcc cac cta cgc att 31060 Met Asp Gly Arg Ala Ser Glu Gln Arg Leu Ala His Leu Arg Ile 6150 6155 6160 cgc cag cag cag gaa cgt gca gtc aag gag ctc agg gat gcc att 31105 Arg Gln Gln Gln Glu Arg Ala Val Lys Glu Leu Arg Asp Ala Ile 6165 6170 6175 gag att cac cag tgc aaa aaa ggc ata ttc tgc ttg gta aaa caa 31150 Glu Ile His Gln Cys Lys Lys Gly Ile Phe Cys Leu Val Lys Gln 6180 6185 6190 gcc aag atc tca tac gag atc acc gct aac gac cac cgc ctc tca 31195 Ala Lys Ile Ser Tyr Glu Ile Thr Ala Asn Asp His Arg Leu Ser 6195 6200 6205 tat gag ctt ggc ctg cag cgt cag aaa ttc acc tgc atg gtt gga 31240 Tyr Glu Leu Gly Leu Gln Arg Gln Lys Phe Thr Cys Met Val Gly 6210 6215 6220 atc aat ccc ata gtc atc acc cag caa gct gga gat acc aag ggt 31285 Ile Asn Pro Ile Val Ile Thr Gln Gln Ala Gly Asp Thr Lys Gly 6225 6230 6235 tgt atc cat tgt tcc tgt gaa tcc acc gag tgc atc tac acc ctg 31330 Cys Ile His Cys Ser Cys Glu Ser Thr Glu Cys Ile Tyr Thr Leu 6240 6245 6250 ctg aag acc ctc tgc ggc ctt cga gac cta cta ccc atg aac 31372 Leu Lys Thr Leu Cys Gly Leu Arg Asp Leu Leu Pro Met Asn 6255 6260 6265 taatcaataa ccccctaccc ctttccatca aacccaaaaa acaattaata aaatcactta 31432 cttaaaatca gaaacaaggt ttttgtccaa gttgttttta agcagcacct cacttccctc 31492 ttcccaactc tggtactcta agcctcggcg ggtggcatac ttcctccaca ctttaaaagg 31552 gatgtcaaat tttagttcct cttctttgcc cacaatcttc atttctttat ccccag atg 31611 Met gcc aaa cga gct cga cta agc agc tcc ttc aat ccg gtc tac ccc 31656 Ala Lys Arg Ala Arg Leu Ser Ser Ser Phe Asn Pro Val Tyr Pro 6270 6275 6280 tat gaa gac gaa agc agc tca caa cac cca ttt ata aac cct ggc 31701 Tyr Glu Asp Glu Ser Ser Ser Gln His Pro Phe Ile Asn Pro Gly 6285 6290 6295 ttc att tcc cct aat ggg ttt aca caa agt cca gac gga gct ctt 31746 Phe Ile Ser Pro Asn Gly Phe Thr Gln Ser Pro Asp Gly Ala Leu 6300 6305 6310 aca ctc aag tgt gtt gct cct ctt act acc acc agt ggc gcc ctg 31791 Thr Leu Lys Cys Val Ala Pro Leu Thr Thr Thr Ser Gly Ala Leu 6315 6320 6325 gac att aaa gta gga ggg ggg ctt aag gta gac tcc act gat ggg 31836 Asp Ile Lys Val Gly Gly Gly Leu Lys Val Asp Ser Thr Asp Gly 6330 6335 6340 tcc tta gaa gaa aac ata ggc act aca gaa cca ctc aac aaa tct 31881 Ser Leu Glu Glu Asn Ile Gly Thr Thr Glu Pro Leu Asn Lys Ser 6345 6350 6355 aat cat tcc ata gga tta gca gtg gga aat gga tta caa aca aat 31926 Asn His Ser Ile Gly Leu Ala Val Gly Asn Gly Leu Gln Thr Asn 6360 6365 6370 gaa agc aaa cta tgt gcc aaa tta gga gat gga ctt att ttt gac 31971 Glu Ser Lys Leu Cys Ala Lys Leu Gly Asp Gly Leu Ile Phe Asp 6375 6380 6385 tct tcc aac gcc atc gca ata aaa aac aac act tta tgg aca gga 32016 Ser Ser Asn Ala Ile Ala Ile Lys Asn Asn Thr Leu Trp Thr Gly 6390 6395 6400 gca aaa cca gaa gct aat tgc ata att gag tat gga aaa gaa agc 32061 Ala Lys Pro Glu Ala Asn Cys Ile Ile Glu Tyr Gly Lys Glu Ser 6405 6410 6415 aca gac agc aag ctt act cta gtt ctt gta aaa aat gga gga att 32106 Thr Asp Ser Lys Leu Thr Leu Val Leu Val Lys Asn Gly Gly Ile 6420 6425 6430 gta aat ggc tat gtg acc cta atg gga gcc tcg gac tat gtt aat 32151 Val Asn Gly Tyr Val Thr Leu Met Gly Ala Ser Asp Tyr Val Asn 6435 6440 6445 aca tta ttt aca aac aag tat gcc tcc att aat gta gaa cta tac 32196 Thr Leu Phe Thr Asn Lys Tyr Ala Ser Ile Asn Val Glu Leu Tyr 6450 6455 6460 ttt gac gcc aat ggt cac ctc cta aca gac tcg tct tct ctc aaa 32241 Phe Asp Ala Asn Gly His Leu Leu Thr Asp Ser Ser Ser Leu Lys 6465 6470 6475 act gat tta caa cta aaa tcc caa acc act gag tct agt aca aaa 32286 Thr Asp Leu Gln Leu Lys Ser Gln Thr Thr Glu Ser Ser Thr Lys 6480 6485 6490 ggt ttt atg ccc agt act ata gca tat cca ttt gtc ctt cct aat 32331 Gly Phe Met Pro Ser Thr Ile Ala Tyr Pro Phe Val Leu Pro Asn 6495 6500 6505 gct gga aga gat aat gaa gac tat att tat ggt caa tgc tat tac 32376 Ala Gly Arg Asp Asn Glu Asp Tyr Ile Tyr Gly Gln Cys Tyr Tyr 6510 6515 6520 aaa gca agt agt gat gga acc ctt ttt cca ctg gaa gtt act gtt 32421 Lys Ala Ser Ser Asp Gly Thr Leu Phe Pro Leu Glu Val Thr Val 6525 6530 6535 atg ctt aat aaa cgc ctg cca gat agt agg aca tcc tat gtt atg 32466 Met Leu Asn Lys Arg Leu Pro Asp Ser Arg Thr Ser Tyr Val Met 6540 6545 6550 acc ttc tct tgg tct ttg aat gca act caa gca cca gaa acc act 32511 Thr Phe Ser Trp Ser Leu Asn Ala Thr Gln Ala Pro Glu Thr Thr 6555 6560 6565 caa gcc acc ctc att acc tcc ccc ttc ttt ttt tct tac att aga 32556 Gln Ala Thr Leu Ile Thr Ser Pro Phe Phe Phe Ser Tyr Ile Arg 6570 6575 6580 gaa gat gac tgacaacaaa aaaataaagt tcaacttttt tattgaacaa 32605 Glu Asp Asp tcagtttaca ggattcgagt agttattttg cctccccctt cccatttcat agaatacacc 32665 aatctctccc cacgcacagc tttaaacatt tggattccat ttgagatagt catggatttg 32725 gattccacat tccacacagt ttcagagcta gataatcttg gatcagtgat agatataaat 32785 ccatcggggc agtccttcaa ggtgatttca cagtccagtt gctgtggctg cggctccgga 32845 gtctggatca gagtcatctg gaagaagaac gatgggagtc ataatccgag aacgggatcg 32905 ggcggttgtg tctcatcaaa ccccgaagca gtcgctgtct gcgccgctcc gtacgactgc 32965 tgctgatggg atcggggtcc acagtctctc gaagcatgat tctaatagcc ctcaacatta 33025 acattctggt acgatgcgca cagcagcgca tcctgatctc acttaagtca cagcagtagg 33085 tacaacacaa caccacaatg ttgtttaaca ggccataatt aaaggcgctc cagccaaaac 33145 tcatttcagg aataatttgc ccagcgtggc catcgtacca aatcctgatg taaatcaaat 33205 ggcgccccct ccagaacaca ctgcccacat acatgatctc cttaggcata tgcatattca 33265 caatctctcg gtaccatgga cagcgctggt taatcatgca gccccaaata accttccgga 33325 accaaatggc cagcactgcg cccccagcaa tacattgaag agaaccctgt cgattacagt 33385 gacaatggag aacccacttt tctcgcccat ggattacttg ggaataaaat atatctattg 33445 tggcacaaca cagacataaa tgcatacatc ttctcatcac ccttaactct tcaggggtta 33505 aaaacatatc ccagggaata ggaagctctt gcaaaacagt gaaggtggca gaacaaggca 33565 gaccgcgaac ataacttaca ctgtgcatgg tcaaggtatt gcaatctggt aacagcggat 33625 gctcctcagt catagaagct ctggtttcac tttcctcaca gcgtggtaaa ggggccctca 33685 gttgaggttc cctggtgtaa ggatggtgtc tggcgcacga tgtcgagcgt gcccgcgacc 33745 tcgttgtaat ggagcttctt cctgacattc tcgtattttg caaagcagaa cctagtcttg 33805 gcacagcaca cgtcccgtcg cctcctgtcc cgccgcctag cacgttcagt gtggtaatta 33865 tagtacaacc attcccgtag attggtcaaa agatcttcag cctcagttgt cataaaaact 33925 ccatcatatc ttactgctct gataaaatca ttcacggtag aaagtgcaat gcccagccag 33985 gcaatgcaat tagcttgtgt ttcaaccaaa ggagggggag gaagacatgg aagaaccata 34045 attaattttt atgccagacg atcccgcagt atttctatat ggagatcacg gagatggcac 34105 ctctcgcccc cactgtgttg atgaaaaatg acagctaggt caaacataat gcgattttcc 34165 aggtgctcaa cggtggcttc aagcaaagcc tccaaacgta catccaaaaa caaaagaaca 34225 gcaaaagcag gagcattttc taattcctca atcatcatat tacattcctg taccattccc 34285 aaataatttt catctttcca tccttgaatt attcgtgtta tttcatctgg taaatccaat 34345 ccacacatga gaaatagctc ccggagggcg ccctccaccg gtaatcttaa gcacaccctc 34405 atagtgagaa aatatcgtgc tcctctgtca cctgcagcaa attgagaatg gcaacatcat 34465 actggatgcc actggctcta agttcttctc taagttccag ttgtaaaaac tcttgcatat 34525 catcgccaaa ctgcttagcc ataggtcctc caggaataag agcgggggac gctacagtgc 34585 agaacaagcg catgccaccc caattgcctc cagcaaaagt gaggttgcaa taagcatact 34645 gagaacctcc agtaatatca tccagtgtac tggaaagata atcaggcaga gcttctctta 34705 tacaattaat aatagaaaag tctgccagat gaacatttaa agcctgtggg atgcagatgc 34765 aataagttat cgcgctgcgc tccaacattg ttagtatggt tagtctgtaa aaacaaaaaa 34825 acaaaaaatt acatcacgct gtgctggcga acgggtggat aaatcactct ctccatcacc 34885 aggcaggcta cagggtctcc agcgcgaccc tcgtaaaacc tatcagtatg attaaaaagc 34945 atcaccgaaa ggggttgttg atggccagca tatattattt gcgatgaagc atacaaacca 35005 gaagtgttag tatcagttaa agaaaaaaat cggccaagat agcatctcgg aacgattatg 35065 ctcaatctca aatgcagcaa agcgacacca cgcggatgca aagtaaaatc cacaggagca 35125 taaaaaaagt aattattccc ctcttgcaca ggcagtctag ctcccggtcc ctccaaaatc 35185 acatataaag cttcagcagc catagcttac cgcgcaaatc aggcacagca gtcagataga 35245 gaaaaagctg tgaactgact gcccagcctg tgcgcaatat atagagaacc cttacactga 35305 cgtaattggg caaagtctaa aaaatcccgc caaaaaaaac agcacacgcc caaaagtgtg 35365 tcactcgcta aaaaaatatt tttcacttcc tcgttccgta tatgacgtca attccgcttt 35425 cccacgaatc gtcacttccg gccatcttgc agcgtcacct ccccgcgccg gcccgcccct 35485 tttgaccgtt gaacccgctg gccaatcccc ttccgccctc cattttcaaa agctcatttg 35545 catgttggca ccgttccatt tataaggtat attattgatg atg 35588 <210> 104 <211> 495 <212> PRT <213> Simian adenovirus 32 <400> 104 Met Asp Pro Thr Asn Pro Leu Gln Gln Gly Ile Arg Leu Gly Phe His 1 5 10 15 Ser Ser Ser Phe Val Glu Asn Met Glu Gly Pro Gln Ala Glu Asp Asn 20 25 30 Leu Arg Leu Leu Ala Ser Ala Ala Ser Gly Arg Ser Ser Asn Pro Glu 35 40 45 Thr Pro Thr Gly His Ala Ser Gly Phe Gly Gly Gly Ala Ala Gly Gly 50 55 60 Gln Pro Glu Ser Arg Pro Gly Pro Ser Gly Gly Gly Gly Gly Gly Val 65 70 75 80 Ala Asp Leu Phe Pro Glu Leu Arg Arg Val Leu Thr Arg Ser Thr Ser 85 90 95 Ser Gly Gln Asp Arg Gly Ile Lys Arg Glu Arg Asn Ala Ser Gly His 100 105 110 Asn Ser Arg Thr Glu Leu Ala Leu Ser Leu Met Ser Arg Ser Arg Pro 115 120 125 Glu Thr Ile Trp Trp His Glu Val Gln Ser Glu Gly Arg Asp Glu Val 130 135 140 Ser Ile Leu Gln Glu Lys Tyr Ser Leu Glu Gln Ile Lys Thr Cys Trp 145 150 155 160 Leu Glu Pro Glu Asp Asp Trp Glu Val Ala Ile Arg Asn Tyr Ala Lys 165 170 175 Ile Ser Leu Arg Pro Asp Lys Gln Tyr Lys Ile Thr Lys Lys Ile Asn 180 185 190 Ile Arg Asn Ala Cys Tyr Ile Ala Gly Asn Gly Ala Glu Val Ile Ile 195 200 205 Asp Thr Pro Asp Lys Thr Ala Phe Arg Cys Cys Met Met Gly Met Trp 210 215 220 Pro Gly Val Ala Gly Met Glu Ala Val Thr Leu Met Asn Ile Arg Phe 225 230 235 240 Arg Gly Asp Gly Tyr Asn Gly Ile Val Phe Met Ala Asn Thr Lys Leu 245 250 255 Ile Leu His Gly Cys Ser Phe Phe Gly Phe Asn Asn Thr Cys Val Glu 260 265 270 Ser Trp Gly Gln Val Ser Ile Arg Gly Cys Ser Phe Tyr Ala Cys Trp 275 280 285 Ile Ala Leu Ser Gly Arg Thr Lys Ser Gln Leu Ser Val Lys Lys Cys 290 295 300 Met Phe Glu Arg Cys Asn Leu Gly Ile Leu Asn Glu Gly Glu Ala Arg 305 310 315 320 Val Arg His Cys Ala Ala Thr Glu Thr Gly Cys Phe Ile Leu Ile Lys 325 330 335 Gly Asn Ala Ser Val Arg His Asn Met Ile Cys Gly Pro Ser Asp Glu 340 345 350 Arg Pro Tyr Gln Met Leu Thr Cys Ala Gly Gly His Cys Asn Met Leu 355 360 365 Ala Thr Val His Ile Val Ser His Ala Arg Lys Lys Trp Pro Val Phe 370 375 380 Glu His Asn Val Met Thr Lys Cys Thr Met His Ile Gly Gly Arg Arg 385 390 395 400 Gly Met Phe Met Pro Tyr Gln Cys Asn Met Asn His Val Lys Val Met 405 410 415 Leu Glu Pro Asp Ala Phe Ser Arg Met Ser Leu Thr Gly Ile Phe Asp 420 425 430 Met Asn Val Gln Leu Trp Lys Ile Leu Arg Tyr Asp Glu Thr Lys Ser 435 440 445 Arg Val Arg Ala Cys Glu Cys Gly Gly Lys His Ala Arg Phe Gln Pro 450 455 460 Val Cys Val Asp Val Thr Glu Asp Leu Arg Pro Asp His Leu Val Leu 465 470 475 480 Ala Cys Thr Gly Ala Glu Phe Gly Ser Ser Gly Glu Glu Thr Asp 485 490 495 <210> 105 <211> 138 <212> PRT <213> Simian adenovirus 32 <400> 105 Met Ser Gly Ser Ala Ser Phe Glu Gly Gly Val Phe Ser Pro Tyr Leu 1 5 10 15 Thr Gly Arg Leu Pro Pro Trp Ala Gly Val Arg Gln Asn Val Met Gly 20 25 30 Ser Thr Val Asp Gly Arg Pro Val Gln Pro Ala Asn Ser Ser Thr Leu 35 40 45 Thr Tyr Ala Thr Leu Ser Ser Ser Pro Leu Asp Ala Ala Ala Ala Ala 50 55 60 Ala Ala Ser Ala Ala Ala Asn Thr Val Leu Gly Met Gly Tyr Tyr Gly 65 70 75 80 Ser Ile Val Ala Asn Ser Ser Ser Ser Asn Asn Pro Ser Thr Leu Ala 85 90 95 Glu Asp Lys Leu Leu Val Leu Leu Ala Gln Leu Glu Ala Leu Thr Gln 100 105 110 Arg Leu Gly Glu Leu Ser Gln Gln Val Ala Gln Leu Arg Glu Gln Thr 115 120 125 Glu Ser Ala Val Ala Thr Ala Lys Ser Lys 130 135 <210> 106 <211> 389 <212> PRT <213> Simian adenovirus 32 <400> 106 Met His Pro Val Leu Arg Gln Met Arg Pro Gln Gln Gln Ala Pro Ser 1 5 10 15 Gln Gln Gln Gln Gln Pro Gln Lys Ala Leu Pro Ala Pro Ala Pro Ala 20 25 30 Thr Thr Ala Ala Ala Ala Val Cys Gly Ala Gly Gln Ser Ala Tyr Asp 35 40 45 Leu Asp Leu Glu Glu Gly Glu Gly Leu Ala Arg Leu Gly Ala Pro Ser 50 55 60 Pro Glu Arg His Pro Arg Val Gln Leu Lys Lys Asp Ser Arg Glu Ala 65 70 75 80 Tyr Val Pro Gln Gln Asn Leu Phe Arg Asp Arg Ser Gly Glu Glu Pro 85 90 95 Glu Glu Met Arg Ala Ser Arg Phe Asn Ala Gly Arg Glu Leu Arg His 100 105 110 Gly Leu Asp Arg Arg Arg Val Leu Arg Asp Glu Asp Phe Glu Val Asp 115 120 125 Glu Val Thr Gly Ile Ser Pro Ala Arg Ala His Val Ala Ala Ala Asn 130 135 140 Leu Val Ser Ala Tyr Glu Gln Thr Val Lys Glu Glu Arg Asn Phe Gln 145 150 155 160 Lys Ser Phe Asn Asn His Val Arg Thr Leu Ile Ala Arg Glu Glu Val 165 170 175 Thr Leu Gly Leu Met His Leu Trp Asp Leu Met Glu Ala Ile Thr Gln 180 185 190 Asn Pro Thr Ser Lys Pro Leu Thr Ala Gln Leu Phe Leu Val Val Gln 195 200 205 His Ser Arg Asp Asn Glu Ala Phe Arg Glu Ala Leu Leu Asn Ile Thr 210 215 220 Glu Pro Glu Gly Arg Trp Leu Tyr Asp Leu Ile Asn Ile Leu Gln Ser 225 230 235 240 Ile Ile Val Gln Glu Arg Ser Leu Gly Leu Ala Glu Lys Val Ala Ala 245 250 255 Ile Asn Tyr Ser Val Leu Ser Leu Gly Lys Tyr Tyr Ala Arg Lys Ile 260 265 270 Tyr Lys Thr Pro Tyr Val Pro Ile Asp Lys Glu Val Lys Ile Asp Gly 275 280 285 Phe Tyr Met Arg Met Thr Leu Lys Val Leu Thr Leu Ser Asp Asp Leu 290 295 300 Gly Val Tyr Arg Asn Asp Arg Met His Arg Ala Val Ser Ala Ser Arg 305 310 315 320 Arg Arg Glu Leu Ser Asp Arg Glu Leu Met His Ser Leu Gln Arg Ala 325 330 335 Leu Thr Gly Ala Gly Thr Glu Gly Glu Asn Tyr Phe Asp Met Gly Ala 340 345 350 Asp Leu Gln Trp Gln Pro Ser Arg Arg Ala Leu Asp Ala Ala Gly Cys 355 360 365 Glu Leu Pro Tyr Ile Glu Glu Val Asp Glu Gly Glu Glu Glu Glu Gly 370 375 380 Glu Tyr Leu Glu Asp 385 <210> 107 <211> 587 <212> PRT <213> Simian adenovirus 32 <400> 107 Met Glu Gln Gln Ala Pro Asp Pro Ala Met Arg Ala Ala Leu Gln Ser 1 5 10 15 Gln Pro Ser Gly Ile Asn Ser Ser Asp Asp Trp Thr Gln Ala Met Gln 20 25 30 Arg Ile Met Ala Leu Thr Thr Arg Asn Pro Glu Ala Phe Arg Gln Gln 35 40 45 Pro Gln Ala Asn Arg Leu Ser Ala Ile Leu Glu Ala Val Val Pro Ser 50 55 60 Arg Ser Asn Pro Thr His Glu Lys Val Leu Ala Ile Val Asn Ala Leu 65 70 75 80 Val Glu Asn Lys Ala Ile Arg Pro Asp Glu Ala Gly Leu Val Tyr Asn 85 90 95 Ala Leu Leu Glu Arg Val Ala Arg Tyr Asn Ser Ser Asn Val Gln Thr 100 105 110 Asn Leu Asp Arg Met Val Thr Asp Val Arg Glu Ala Val Ser Gln Arg 115 120 125 Glu Arg Phe Gln Arg Asp Ala Asn Leu Gly Ser Leu Val Ala Leu Asn 130 135 140 Ala Phe Leu Ser Thr Gln Pro Ala Asn Val Pro Arg Gly Gln Gln Asp 145 150 155 160 Tyr Thr Asn Phe Leu Ser Ala Leu Arg Leu Met Val Thr Glu Val Pro 165 170 175 Gln Ser Glu Val Tyr Gln Ser Gly Pro Asp Tyr Phe Phe Gln Thr Ser 180 185 190 Arg Gln Gly Leu Gln Thr Val Asn Leu Ser Gln Ala Phe Lys Asn Leu 195 200 205 Arg Gly Leu Trp Gly Val His Ala Pro Val Gly Asp Arg Ala Thr Val 210 215 220 Ser Ser Leu Leu Thr Pro Asn Ser Arg Leu Leu Leu Leu Leu Val Ser 225 230 235 240 Pro Phe Thr Asp Ser Gly Ser Ile Asp Arg Asn Ser Tyr Leu Gly Tyr 245 250 255 Leu Leu Asn Leu Tyr Arg Glu Ala Ile Gly Gln Ser Gln Val Asp Glu 260 265 270 Gln Thr Tyr Gln Glu Ile Thr Gln Val Ser Arg Ala Leu Gly Gln Glu 275 280 285 Asp Thr Gly Ser Leu Glu Ala Thr Leu Asn Phe Leu Leu Thr Asn Arg 290 295 300 Ser Gln Lys Ile Pro Pro Gln Tyr Ala Leu Thr Ala Glu Glu Glu Arg 305 310 315 320 Ile Leu Arg Tyr Val Gln Gln Ser Val Gly Leu Phe Leu Met Gln Glu 325 330 335 Gly Ala Thr Pro Ser Ala Ala Leu Asp Met Thr Ala Arg Asn Met Glu 340 345 350 Pro Ser Met Tyr Ala Ser Asn Arg Pro Phe Ile Asn Lys Leu Leu Asp 355 360 365 Tyr Leu His Arg Ala Ala Ala Met Asn Ser Asp Tyr Phe Thr Asn Ala 370 375 380 Ile Leu Asn Pro His Trp Leu Pro Pro Pro Gly Phe Tyr Thr Gly Glu 385 390 395 400 Tyr Asp Met Pro Asp Pro Asn Asp Gly Phe Leu Trp Asp Asp Val Asp 405 410 415 Ser Ser Ile Phe Ser Pro Pro Pro Gly Tyr Asn Thr Trp Lys Lys Glu 420 425 430 Gly Gly Asp Arg Arg His Ser Ser Val Ser Leu Ser Gly Ser Arg Gly 435 440 445 Ala Ala Ala Ala Val Pro Glu Ala Ala Ser Pro Phe Pro Ser Leu Pro 450 455 460 Phe Ser Leu Asn Ser Val Arg Ser Ser Glu Leu Gly Arg Ile Thr Arg 465 470 475 480 Pro Arg Leu Met Gly Glu Asp Glu Tyr Leu Asn Asp Ser Leu Leu Arg 485 490 495 Pro Glu Arg Glu Lys Asn Phe Pro Asn Asn Gly Ile Glu Ser Leu Val 500 505 510 Asp Lys Met Ser Arg Trp Lys Thr Tyr Ala Gln Asp His Lys Asp Glu 515 520 525 Pro Arg Ile Leu Gly Ala Ala Ser Gly Thr Thr Arg Arg Arg Gln Arg 530 535 540 His Asp Arg Gln Arg Gly Leu Val Trp Asp Asp Glu Asp Ser Ala Asp 545 550 555 560 Asp Ser Ser Val Leu Asp Leu Gly Gly Arg Gly Gly Gly Asn Pro Phe 565 570 575 Ala His Leu Arg Pro His Phe Gly Arg Met Leu 580 585 <210> 108 <211> 585 <212> PRT <213> Simian adenovirus 32 <400> 108 Met Ser Ser Met Met Arg Arg Ala Val Leu Gly Gly Ala Val Val Tyr 1 5 10 15 Pro Glu Gly Pro Pro Pro Ser Tyr Glu Ser Val Met Gln Gln Gln Ala 20 25 30 Ala Ala Val Met Gln Pro Ser Leu Glu Ala Pro Phe Val Pro Pro Arg 35 40 45 Tyr Leu Ala Pro Thr Glu Gly Arg Asn Ser Ile Arg Tyr Ser Glu Leu 50 55 60 Ala Pro Gln Tyr Asp Thr Thr Arg Leu Tyr Leu Val Asp Asn Lys Ser 65 70 75 80 Ala Asp Ile Ala Ser Leu Asn Tyr Gln Asn Asp His Ser Asn Phe Leu 85 90 95 Thr Thr Val Val Gln Asn Asn Asp Phe Thr Pro Thr Glu Ala Ser Thr 100 105 110 Gln Thr Ile Asn Phe Asp Glu Arg Ser Arg Trp Gly Gly Gln Leu Lys 115 120 125 Thr Ile Met His Thr Asn Met Pro Asn Val Asn Glu Tyr Met Phe Ser 130 135 140 Asn Lys Phe Lys Ala Arg Val Met Val Ser Arg Lys Ala Pro Glu Gly 145 150 155 160 Val Thr Val Asp Asp Thr Tyr Asp His Lys Gln Asp Ile Leu Glu Tyr 165 170 175 Glu Trp Phe Glu Phe Thr Leu Pro Glu Gly Asn Phe Ser Ala Thr Met 180 185 190 Thr Ile Asp Leu Met Asn Asn Ala Ile Ile Asp Asn Tyr Leu Glu Ile 195 200 205 Gly Arg Gln Asn Gly Val Leu Glu Ser Asp Ile Gly Val Lys Phe Asp 210 215 220 Thr Arg Asn Phe Arg Leu Gly Trp Asp Pro Glu Thr Lys Leu Ile Met 225 230 235 240 Pro Gly Val Tyr Thr Tyr Glu Ala Phe His Pro Asp Ile Val Leu Leu 245 250 255 Pro Gly Cys Gly Val Asp Phe Thr Glu Ser Arg Leu Ser Asn Leu Leu 260 265 270 Gly Ile Arg Lys Arg His Pro Phe Gln Glu Gly Phe Gln Ile Leu Tyr 275 280 285 Glu Asp Leu Glu Gly Gly Asn Ile Pro Ala Leu Leu Asp Val Glu Thr 290 295 300 Tyr Glu Lys Ser Lys Lys Glu Asn Ala Gly Thr Thr Thr Glu Gly Thr 305 310 315 320 Thr Thr Val Ala Val Ala Asn Ala Leu Thr Thr Ala Lys Ala Ala Ala 325 330 335 Asn Val Thr Val Asp Val Ile Thr Glu Ile Asn Asn Asn Ser Val Arg 340 345 350 Gly Asp Asn Tyr Leu Ser Ala Asn Asp Met Lys Asp Ser Ser Glu Thr 355 360 365 Thr Val Glu Pro Ala Val Pro Ile Val Val Pro Gly Thr Lys Thr Glu 370 375 380 Thr Glu Thr Glu Thr Lys Thr Pro Thr Ile Gln Pro Leu Lys Lys Asp 385 390 395 400 Ser Lys Ser Arg Ser Tyr Asn Val Leu Glu Asp Glu Val Asn Thr Ala 405 410 415 Tyr Arg Ser Trp Tyr Leu Ser Tyr Asn Tyr Gly Asp Pro Glu Lys Gly 420 425 430 Val Arg Ser Trp Thr Leu Leu Thr Thr Ser Asp Val Thr Cys Gly Ala 435 440 445 Glu Gln Val Tyr Trp Ser Leu Pro Asp Met Met Gln Asp Pro Val Thr 450 455 460 Phe Arg Ser Thr Arg Gln Val Ser Asn Tyr Pro Val Val Gly Ala Glu 465 470 475 480 Leu Met Pro Val Phe Ser Lys Ser Phe Tyr Asn Glu Gln Ala Val Tyr 485 490 495 Ser Gln Gln Leu Arg Gln Thr Thr Ser Leu Thr His Val Phe Asn Arg 500 505 510 Phe Pro Glu Asn Gln Ile Leu Ile Arg Pro Pro Ala Pro Thr Ile Thr 515 520 525 Thr Val Ser Glu Asn Val Pro Ala Leu Thr Asp His Gly Thr Leu Pro 530 535 540 Leu Arg Ser Ser Ile Arg Gly Val Gln Arg Val Thr Val Thr Asp Ala 545 550 555 560 Arg Arg Arg Thr Cys Pro Tyr Val Tyr Lys Ala Leu Gly Ile Val Ala 565 570 575 Pro Arg Val Leu Ser Ser Arg Thr Phe 580 585 <210> 109 <211> 192 <212> PRT <213> Simian adenovirus 32 <400> 109 Met Ser Ile Leu Ile Ser Pro Ser Asn Asn Thr Gly Trp Gly Leu Arg 1 5 10 15 Thr Pro Thr Arg Met Tyr Gly Gly Ala Arg Lys Arg Ser Thr Gln His 20 25 30 Pro Val Arg Val Arg Gly His Phe Arg Ala Pro Trp Gly Ala Leu Lys 35 40 45 Gly Arg Ala Arg Thr Arg Thr Thr Val Asp Asp Val Ile Asp Gln Val 50 55 60 Val Ala Asp Ala Arg Asn Tyr Thr Pro Ala Ala Pro Ala Ser Thr Val 65 70 75 80 Asp Ala Val Ile Asp Ser Val Val Ala Asp Ala Arg Asp Tyr Ala Arg 85 90 95 Arg Lys Ser Arg Arg Arg Arg Ile Ala Arg Arg His Arg Thr Thr Pro 100 105 110 Ala Met Arg Ala Ala Arg Ala Leu Leu Arg Lys Ala Lys Arg Val Gly 115 120 125 Arg Arg Ala Met Leu Arg Ala Ala Arg Arg Ala Ala Ser Gly Ala Ile 130 135 140 Ala Gly Arg Ser Arg Arg Arg Ala Ala Thr Ala Ala Ala Ala Ala Ile 145 150 155 160 Ala Asn Met Ala Gln Pro Arg Arg Gly Asn Val Tyr Trp Val Arg Asp 165 170 175 Ala Thr Thr Gly Gln Arg Val Pro Val Arg Thr Arg Pro Pro Arg Thr 180 185 190 <210> 110 <211> 350 <212> PRT <213> Simian adenovirus 32 <400> 110 Met Ser Lys Arg Lys Tyr Lys Glu Glu Met Leu Gln Val Ile Ala Pro 1 5 10 15 Glu Ile Tyr Gly Pro Pro Val Lys Asp Glu Lys Lys Pro Arg Lys Ile 20 25 30 Lys Arg Val Lys Lys Asp Lys Lys Glu Glu Asp Gly Asp Asp Gly Leu 35 40 45 Val Glu Phe Val Arg Glu Phe Ala Pro Arg Arg Arg Val Gln Trp Arg 50 55 60 Gly Arg Arg Val Arg Pro Val Leu Arg Pro Gly Thr Thr Val Val Phe 65 70 75 80 Thr Pro Gly Glu Arg Ser Ser Thr Thr Phe Lys Arg Ser Tyr Asp Glu 85 90 95 Val Tyr Gly Asp Asp Asp Ile Leu Glu Gln Ala Ala Asp Arg Leu Gly 100 105 110 Glu Phe Ala Tyr Gly Lys Arg Ser Arg Ser Ser Pro Lys Glu Glu Ala 115 120 125 Val Ser Ile Pro Leu Asp His Gly Asn Pro Thr Pro Ser Leu Lys Pro 130 135 140 Val Thr Leu Gln Gln Val Leu Pro Val Pro Pro Arg Arg Gly Val Lys 145 150 155 160 Arg Glu Gly Glu Asp Leu Tyr Pro Thr Met Gln Leu Met Val Pro Lys 165 170 175 Arg Gln Lys Leu Glu Asp Val Leu Glu Lys Met Lys Val Asp Pro Asp 180 185 190 Ile Gln Pro Glu Val Lys Val Arg Pro Ile Lys Gln Val Ala Pro Gly 195 200 205 Leu Gly Val Gln Thr Val Asp Ile Lys Ile Pro Thr Glu Ser Met Glu 210 215 220 Val Gln Thr Glu Pro Ala Lys Pro Thr Ala Thr Ser Ile Glu Val Gln 225 230 235 240 Thr Asp Pro Trp Met Pro Ala Pro Ile Ala Thr Thr Ala Ser Thr Ala 245 250 255 Arg Arg Pro Arg Arg Lys Tyr Gly Pro Ala Ser Leu Leu Met Pro Asn 260 265 270 Tyr Ala Leu His Pro Ser Ile Ile Pro Thr Pro Gly Tyr Arg Gly Thr 275 280 285 Arg Tyr Tyr Arg Ser Arg Ser Thr Thr Ser Arg Arg Arg Lys Thr Pro 290 295 300 Ala Ser Arg Ser Arg Arg Arg Arg Arg Arg Ala Thr Ser Lys Leu Thr 305 310 315 320 Pro Ala Ala Leu Val Arg Arg Val Tyr Arg Asp Gly Arg Ala Glu Pro 325 330 335 Leu Met Leu Pro Arg Ala Arg Tyr His Pro Ser Ile Thr Thr 340 345 350 <210> 111 <211> 75 <212> PRT <213> Simian adenovirus 32 <400> 111 Met Ala Leu Thr Cys Arg Leu Arg Val Pro Ile Thr Gly Tyr Arg Gly 1 5 10 15 Arg Asn Ser Arg Arg Arg Arg Met Leu Gly Arg Gly Met Arg Arg His 20 25 30 Arg Arg Arg Arg Ala Ile Ser Lys Arg Leu Gly Gly Gly Phe Leu Thr 35 40 45 Ala Leu Ile Pro Ile Ile Ala Ala Ala Ile Gly Ala Val Pro Gly Ile 50 55 60 Ala Ser Val Ala Val Gln Ala Ser Gln Arg His 65 70 75 <210> 112 <211> 250 <212> PRT <213> Simian adenovirus 32 <400> 112 Met Glu Asp Ile Asn Phe Ser Ser Leu Ala Pro Arg His Gly Thr Arg 1 5 10 15 Pro Tyr Met Gly Thr Trp Ser Asp Ile Gly Thr Ser Gln Leu Asn Gly 20 25 30 Gly Ala Phe Asn Trp Ser Ser Ile Trp Ser Gly Leu Lys Asn Phe Gly 35 40 45 Ser Thr Ile Lys Thr Tyr Gly Asn Lys Ala Trp Asn Ser Ser Thr Gly 50 55 60 Gln Ala Leu Arg Asn Lys Leu Lys Glu Gln Asn Phe Gln Gln Lys Val 65 70 75 80 Val Asp Gly Ile Ala Ser Gly Ile Asn Gly Val Val Asp Leu Ala Asn 85 90 95 Gln Ala Val Gln Lys Gln Ile Asn Ser Arg Leu Asp Pro Pro Pro Ala 100 105 110 Ala Pro Gly Glu Met Glu Val Glu Glu Glu Leu Pro Pro Leu Glu Lys 115 120 125 Arg Gly Asp Lys Arg Pro Arg Pro Asp Met Glu Glu Thr Leu Val Thr 130 135 140 Arg Gly Asp Glu Pro Pro Pro Tyr Glu Glu Ala Ile Lys Leu Gly Met 145 150 155 160 Pro Thr Thr Arg Pro Ile Ala Pro Met Ala Thr Gly Val Met Lys Pro 165 170 175 Ser Gln Ser His Arg Pro Ala Thr Leu Asp Leu Pro Pro Ala Pro Ala 180 185 190 Ala Ala Ala Pro Ala Pro Lys Pro Val Ala Thr Pro Lys Pro Thr Thr 195 200 205 Val Gln Pro Val Ala Val Ala Arg Pro Arg Pro Gly Gly Thr Pro Arg 210 215 220 Pro Asn Ala Asn Trp Gln Ser Thr Leu Asn Ser Ile Val Gly Leu Gly 225 230 235 240 Val Gln Ser Val Lys Arg Arg Arg Cys Tyr 245 250 <210> 113 <211> 955 <212> PRT <213> Simian adenovirus 32 <400> 113 Met Ala Thr Pro Ser Met Leu Pro Gln Trp Ala Tyr Met His Ile Ala 1 5 10 15 Gly Gln Asp Ala Ser Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala 20 25 30 Arg Ala Thr Asp Thr Tyr Phe Asn Leu Gly Asn Lys Phe Arg Asn Pro 35 40 45 Thr Val Ala Pro Thr His Asp Val Thr Thr Asp Arg Ser Gln Arg Leu 50 55 60 Met Leu Arg Phe Val Pro Val Asp Arg Glu Asp Asn Thr Tyr Ser Tyr 65 70 75 80 Lys Val Arg Tyr Thr Leu Ala Val Gly Asp Asn Arg Val Leu Asp Met 85 90 95 Ala Ser Thr Phe Phe Asp Ile Arg Gly Val Leu Asp Arg Gly Pro Ser 100 105 110 Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser Leu Ala Pro Lys Gly 115 120 125 Ala Pro Asn Thr Ser Gln Trp Leu Ala Glu Gly Thr Asn Asn Ala Ala 130 135 140 Glu Gly Glu Ala Glu Gln Asp Glu Glu Asp Gly Gly Glu Glu Glu Thr 145 150 155 160 Lys Met Ala Thr Tyr Thr Phe Gly Asn Ala Pro Val Lys Ala Asp Ala 165 170 175 Glu Ile Thr Lys Glu Gly Leu Ala Val Gly Val Glu Leu Leu Ala Asp 180 185 190 Asn Asn Thr Lys Pro Ile Tyr Ala Asp Lys Leu Tyr Gln Pro Glu Pro 195 200 205 Gln Val Gly Glu Glu Thr Trp Thr Asp Thr Asp Gly Thr Asn Glu Gln 210 215 220 Tyr Gly Gly Arg Ala Leu Lys Pro Glu Thr Lys Met Lys Pro Cys Tyr 225 230 235 240 Gly Ser Phe Ala Arg Pro Thr Asn Thr Lys Gly Gly Gln Ala Lys Leu 245 250 255 Lys Asn Pro Asp Glu Lys Asp Ile Thr Lys Ile Glu Tyr Asp Val Glu 260 265 270 Met Asp Phe Tyr Glu Leu Lys Ser Gln Val Asn Gly Ser Pro Lys Ile 275 280 285 Val Met Tyr Ala Glu Asn Val Asn Leu Glu Thr Pro Asp Thr His Val 290 295 300 Val Tyr Lys Pro Gly Thr Ser Asp Asp Ser Ser His Ala Asn Leu Gly 305 310 315 320 Gln Gln Ser Met Pro Asn Arg Pro Asn Tyr Ile Gly Phe Arg Asp Asn 325 330 335 Phe Ile Gly Leu Ala Tyr Tyr Asn Ser Thr Gly Asn Met Gly Val Leu 340 345 350 Ala Gly Gln Ala Ser Gln Leu Asn Ala Val Val Asp Leu Gln Asp Arg 355 360 365 Asn Thr Glu Leu Ser Tyr Gln Leu Leu Leu Asp Ser Leu Gly Asp Arg 370 375 380 Thr Arg Tyr Phe Ser Met Trp Asn Gln Ala Val Asp Ser Tyr Asp Pro 385 390 395 400 Asp Val Arg Ile Ile Glu Asn His Gly Val Glu Asp Glu Leu Pro Asn 405 410 415 Tyr Cys Phe Pro Leu Asn Gly Val Gly Ser Glu Thr Glu Arg Tyr Lys 420 425 430 Glu Met Gln Ala Lys Asn Gly Asn Glu Asn Gly Trp Asp Asn Ala Asn 435 440 445 Pro Thr Gly Thr Ser Glu Ile Ala Lys Gly Asn Pro Tyr Ala Met Glu 450 455 460 Ile Asn Leu Gln Ala Asn Leu Trp Arg Ser Phe Leu Tyr Ser Asn Val 465 470 475 480 Ala Leu Tyr Leu Pro Asp Ser Tyr Lys Tyr Thr Pro Ala Asn Ile Thr 485 490 495 Leu Pro Ser Asn Thr Asn Thr Tyr Asp Tyr Leu Asn Gly Arg Val Val 500 505 510 Pro Pro Ser Leu Val Asp Thr Tyr Ile Asn Ile Gly Ala Arg Trp Ser 515 520 525 Leu Asp Ala Met Asp Asn Val Asn Pro Phe Asn His His Arg Asn Ala 530 535 540 Gly Leu Arg Tyr Arg Ser Met Leu Leu Gly Asn Gly Arg Tyr Val Pro 545 550 555 560 Phe His Ile Gln Val Pro Gln Lys Phe Phe Ala Val Lys Asn Leu Leu 565 570 575 Leu Leu Pro Gly Ser Tyr Thr Tyr Glu Trp Asn Phe Arg Lys Asp Val 580 585 590 Asn Met Val Leu Gln Ser Ser Leu Gly Asn Asp Leu Arg Val Asp Gly 595 600 605 Ala Ser Ile Ser Phe Thr Ser Ile Asn Leu Tyr Ala Thr Phe Phe Pro 610 615 620 Met Ala His Asn Thr Ala Ser Thr Leu Glu Ala Met Leu Arg Asn Asp 625 630 635 640 Thr Asn Asp Gln Ser Phe Asn Asp Tyr Leu Ser Ala Ala Asn Met Leu 645 650 655 Tyr Pro Ile Pro Ala Asn Ala Thr Asn Ile Pro Ile Ser Ile Pro Ser 660 665 670 Arg Asn Trp Ala Ala Phe Arg Gly Trp Ser Phe Thr Arg Leu Lys Thr 675 680 685 Lys Glu Thr Pro Ser Leu Gly Ser Gly Phe Asp Pro Tyr Phe Val Tyr 690 695 700 Ser Gly Ser Ile Pro Tyr Leu Asp Gly Thr Phe Tyr Leu Asn His Thr 705 710 715 720 Phe Lys Lys Val Ser Ile Met Phe Asp Ser Ser Val Ser Trp Pro Gly 725 730 735 Asn Asp Arg Leu Leu Ser Pro Asn Glu Phe Glu Ile Lys Arg Thr Val 740 745 750 Asp Gly Glu Gly Tyr Asn Val Ala Gln Cys Asn Met Thr Lys Asp Trp 755 760 765 Phe Leu Val Gln Met Leu Ala Asn Tyr Asn Ile Gly Tyr Gln Gly Phe 770 775 780 Tyr Ile Pro Glu Gly Tyr Lys Asp Arg Met Tyr Ser Phe Phe Arg Asn 785 790 795 800 Phe Gln Pro Met Ser Arg Gln Val Val Asp Glu Val Asn Tyr Thr Asp 805 810 815 Tyr Lys Ala Val Ala Val Pro Tyr Gln His Asn Asn Ser Gly Phe Val 820 825 830 Gly Tyr Met Ala Pro Thr Met Arg Gln Gly Gln Ala Tyr Pro Ala Asn 835 840 845 Tyr Pro Tyr Pro Leu Ile Gly Thr Thr Ala Val Lys Ser Val Thr Gln 850 855 860 Lys Lys Phe Leu Cys Asp Arg Thr Met Trp Arg Ile Pro Phe Ser Ser 865 870 875 880 Asn Phe Met Ser Met Gly Ala Leu Thr Asp Leu Gly Gln Asn Met Leu 885 890 895 Tyr Ala Asn Ser Ala His Ala Leu Asp Met Thr Phe Glu Val Asp Pro 900 905 910 Met Asp Glu Pro Thr Leu Leu Tyr Leu Leu Phe Glu Val Phe Asp Val 915 920 925 Val Arg Val His Gln Pro His Arg Gly Val Ile Glu Ala Val Tyr Leu 930 935 940 Arg Thr Pro Phe Ser Ala Gly Asn Ala Thr Thr 945 950 955 <210> 114 <211> 209 <212> PRT <213> Simian adenovirus 32 <400> 114 Met Ala Cys Gly Ser Gly Asn Gly Ser Ser Glu Gln Glu Leu Arg Ala 1 5 10 15 Ile Ala Arg Asp Leu Gly Cys Gly Pro Tyr Phe Leu Gly Thr Phe Asp 20 25 30 Lys Arg Phe Pro Gly Phe Met Ala Pro Asp Lys Leu Ala Cys Ala Ile 35 40 45 Val Asn Thr Ala Gly Arg Glu Thr Gly Gly Glu His Trp Leu Ala Phe 50 55 60 Gly Trp Asn Pro Arg Ser Asn Thr Cys Tyr Leu Phe Asp Pro Phe Gly 65 70 75 80 Phe Ser Asp Glu Arg Leu Lys Gln Ile Tyr Gln Phe Glu Tyr Glu Gly 85 90 95 Leu Leu Arg Arg Ser Ala Leu Ala Thr Lys Asp Arg Cys Ile Thr Leu 100 105 110 Glu Lys Ser Thr Gln Thr Val Gln Gly Pro Arg Ser Ala Ala Cys Gly 115 120 125 Leu Phe Cys Cys Met Phe Leu His Ala Phe Val His Trp Pro Asp Arg 130 135 140 Pro Met Asp Gly Asn Pro Thr Met Lys Leu Leu Thr Gly Val Pro Asn 145 150 155 160 Ser Met Leu Gln Ser Pro Gln Val Gln Pro Thr Leu Arg His Asn Gln 165 170 175 Glu Ala Leu Tyr Arg Phe Leu Asn Thr His Ser Ser Tyr Phe Arg Ser 180 185 190 His Arg Ala Arg Ile Glu Lys Ala Thr Ala Phe Asp Arg Met Asp Met 195 200 205 Gln <210> 115 <211> 832 <212> PRT <213> Simian adenovirus 32 <400> 115 Met Glu Thr Gln Pro Ser Leu Pro Thr Pro Leu Gln Ala Pro Ser His 1 5 10 15 Leu Ala Pro Ser Ser Asp Glu Glu Glu Gln Ser Leu Thr Thr Pro Pro 20 25 30 Pro Ser Pro Ala Thr Thr Thr Ser Thr Leu Asp Glu Glu Glu Glu Val 35 40 45 Asp Ala Pro Gln Glu Met Gln Val Met Glu Asp Glu Lys Ala Glu Glu 50 55 60 Ile Glu Ala Asp Val Glu Gln Asp Pro Gly Tyr Val Thr Pro Ala Glu 65 70 75 80 His Glu Glu Glu Leu Arg Arg Phe Leu Asp Arg Glu Asp Asn Asn Arg 85 90 95 Pro Glu Gln Gln Ala Asp Gly Asp His Gln Glu Ala Gly Leu Gly Asp 100 105 110 His Val Ala Glu Tyr Leu Thr Gly Leu Gly Gly Glu Asp Val Leu Leu 115 120 125 Lys His Leu Ala Arg Gln Thr Ile Ile Val Lys Asp Ala Leu Leu Asp 130 135 140 Arg Thr Glu Val Pro Ile Ser Val Glu Glu Leu Ser Arg Ala Tyr Glu 145 150 155 160 Leu Asn Leu Phe Ser Pro Arg Val Pro Pro Lys Arg Gln Gln Asn Gly 165 170 175 Thr Cys Glu Pro Asn Pro Arg Leu Asn Phe Tyr Pro Ala Phe Ala Val 180 185 190 Pro Glu Val Leu Ala Thr Tyr His Ile Phe Phe Lys Asn Gln Lys Ile 195 200 205 Pro Val Ser Cys Arg Ala Asn Arg Thr Arg Ala Asp Ala Leu Leu Asn 210 215 220 Leu Gly Pro Gly Ala Arg Leu Pro Asp Ile Ala Ser Leu Glu Glu Val 225 230 235 240 Pro Lys Ile Phe Glu Gly Leu Gly Ser Asp Glu Thr Arg Ala Ala Asn 245 250 255 Ala Leu Gln Gln Gly Glu Asn Gly Met Asp Glu His His Ser Ala Leu 260 265 270 Val Glu Leu Glu Gly Asp Asn Ala Arg Leu Ala Val Leu Lys Arg Ser 275 280 285 Ile Glu Val Thr His Phe Ala Tyr Pro Ala Val Asn Leu Pro Pro Lys 290 295 300 Val Met Ser Ala Val Met Asp Gln Leu Leu Ile Lys Arg Ala Ser Pro 305 310 315 320 Leu Ser Glu Asp Gln Asn Met Gln Asp Pro Asp Ala Ser Asp Glu Gly 325 330 335 Lys Pro Val Val Ser Asp Glu Gln Leu Ser Arg Trp Leu Ser Thr Asn 340 345 350 Ser Pro Arg Asp Leu Glu Glu Arg Arg Lys Leu Met Met Ala Val Val 355 360 365 Leu Val Thr Val Glu Leu Glu Cys Leu Arg Arg Phe Phe Thr Asp Pro 370 375 380 Glu Thr Leu Arg Lys Leu Glu Glu Asn Leu His Tyr Thr Phe Arg His 385 390 395 400 Gly Phe Val Arg Gln Ala Cys Lys Ile Ser Asn Val Glu Leu Thr Asn 405 410 415 Leu Val Ser Tyr Met Gly Ile Leu His Glu Asn Arg Leu Gly Gln Ser 420 425 430 Val Leu His Thr Thr Leu Lys Gly Glu Ala Arg Arg Asp Tyr Ile Arg 435 440 445 Asp Cys Val Tyr Leu Tyr Leu Cys His Thr Trp Gln Thr Gly Met Gly 450 455 460 Val Trp Gln Gln Cys Leu Glu Glu Gln Asn Leu Lys Glu Leu Asp Lys 465 470 475 480 Leu Leu Gln Arg Ser Leu Lys Ala Leu Trp Thr Gly Phe Asp Glu Arg 485 490 495 Thr Val Ala Ser Asp Leu Ala Asp Ile Ile Phe Pro Glu Arg Leu Arg 500 505 510 Val Thr Leu Arg Asn Gly Leu Pro Asp Phe Met Ser Gln Ser Met Leu 515 520 525 Asn Asn Phe Arg Ser Phe Ile Leu Glu Arg Ser Gly Ile Leu Pro Ala 530 535 540 Thr Cys Cys Ala Leu Pro Ser Asp Phe Val Pro Leu Thr Tyr Arg Glu 545 550 555 560 Cys Pro Pro Pro Leu Trp Ser His Cys Tyr Leu Phe Arg Leu Ala Asn 565 570 575 Tyr Leu Ser Tyr His Ser Asp Val Ile Glu Asp Val Ser Gly Asp Gly 580 585 590 Leu Leu Glu Cys His Cys Arg Cys Asn Leu Cys Thr Pro His Arg Ser 595 600 605 Leu Ala Cys Asn Pro Gln Leu Leu Ser Glu Thr Gln Ile Ile Gly Thr 610 615 620 Phe Glu Leu Gln Gly Pro Ser Ser Glu Gly Glu Gly Ser Ser Ser Gly 625 630 635 640 Gln Ser Leu Lys Leu Thr Pro Gly Leu Trp Thr Ser Ala Tyr Leu Arg 645 650 655 Lys Phe Ala Pro Glu Asp Tyr His Pro Tyr Glu Ile Arg Phe Tyr Glu 660 665 670 Asp Gln Ser Gln Pro Pro Lys Ala Glu Leu Ser Ala Cys Val Ile Thr 675 680 685 Gln Gly Ala Ile Leu Ala Gln Leu Gln Ala Ile Gln Lys Ser Arg Gln 690 695 700 Glu Phe Leu Leu Lys Arg Gly Asn Gly Val Tyr Leu Asp Pro Gln Thr 705 710 715 720 Gly Glu Glu Leu Asn Thr Arg Phe Pro Gln Asp Val Pro Ala Pro Arg 725 730 735 Lys Gln Glu Val Glu Gly Ala Ala Ala Ala Pro Arg Gly Tyr Gly Gly 740 745 750 Arg Leu Gly Gln Ser Gly Arg Gly Gly Gly Asp Gly Arg Leu Gly Gln 755 760 765 Pro Gly Arg Gly Gly Gly Gln Pro Gly Gly Arg Gln Phe Gly Gly Gly 770 775 780 Arg Arg Gly Gly Arg Gly Gly Gly Arg Ser Asn Arg Arg Gln Thr Val 785 790 795 800 Val Leu Asp Ser Gly Asp Lys Gln Gly Pro Arg Gln Gln Gln Gln His 805 810 815 Gly Tyr Asn Leu Arg Ser Gly Ser Gly Gly Pro Ala Ala Ser Gln Gln 820 825 830 <210> 116 <211> 227 <212> PRT <213> Simian adenovirus 32 <400> 116 Met Ser Lys Glu Ile Pro Thr Pro Tyr Met Trp Ser Tyr Gln Pro Gln 1 5 10 15 Met Gly Leu Ala Ala Gly Ala Ser Gln Asp Tyr Ser Thr Arg Met Asn 20 25 30 Trp Leu Ser Ala Gly Pro Ser Met Ile Ser Arg Val Asn Asp Ile Arg 35 40 45 Ala Tyr Arg Asn Gln Leu Leu Leu Glu Gln Ser Ala Leu Thr Thr Thr 50 55 60 Pro Arg Gln His Leu Asn Pro Arg Asn Trp Pro Ala Ala Leu Val Tyr 65 70 75 80 Gln Glu Thr Pro Ala Pro Thr Thr Val Leu Leu Pro Arg Asp Ala Gln 85 90 95 Ala Glu Val Gln Met Thr Asn Ala Gly Val Gln Leu Ala Gly Gly Ser 100 105 110 Ala Leu Cys Arg His Arg Pro Gln Gln Ser Ile Lys Arg Leu Val Ile 115 120 125 Arg Gly Arg Gly Ile Gln Leu Asn Asp Glu Ser Val Ser Ser Ser Leu 130 135 140 Gly Leu Arg Pro Asp Gly Val Phe Gln Ile Ala Gly Cys Gly Arg Ser 145 150 155 160 Ser Phe Thr Pro Arg Gln Ala Val Leu Thr Leu Glu Ser Ser Ser Ser 165 170 175 Gln Pro Arg Ser Gly Gly Ile Gly Thr Leu Gln Phe Val Glu Glu Phe 180 185 190 Thr Pro Ser Val Tyr Phe Asn Pro Phe Ser Gly Ser Pro Gly Gln Tyr 195 200 205 Pro Asp Glu Phe Ile Pro Asn Phe Asp Ala Ile Ser Glu Ser Val Asp 210 215 220 Gly Tyr Asp 225 <210> 117 <211> 105 <212> PRT <213> Simian adenovirus 32 <400> 117 Met Ser Gly Gly Ala Ala Glu Leu Ala Arg Leu Arg His Leu Asp His 1 5 10 15 Cys Arg Arg Phe Arg Cys Phe Ala Arg Glu Leu Thr Glu Phe Ile Tyr 20 25 30 Phe Glu Leu Pro Glu Glu His Pro Gln Gly Pro Ala His Gly Val Arg 35 40 45 Ile Thr Ile Glu Gly Gly Ile Asp Ser Arg Leu His Arg Ile Phe Cys 50 55 60 Gln Arg Pro Val Leu Ile Glu Arg Asp Gln Gly Thr Thr Thr Val Ser 65 70 75 80 Ile Tyr Cys Ile Cys Asn His Pro Gly Leu His Glu Ser Leu Cys Cys 85 90 95 Leu Ile Cys Ala Glu Phe Asn Lys Asn 100 105 <210> 118 <211> 178 <212> PRT <213> Simian adenovirus 32 <400> 118 Met Gly Val Val Leu Val Ala Leu Ala Leu Leu Ser Leu Leu Gly Leu 1 5 10 15 Gly Ser Thr Thr Leu Ala Asn Gln Pro Leu Leu Leu Asp Pro Asp Asn 20 25 30 Val Asp Pro Cys Leu Thr Phe Asp Pro Glu Asn Cys Thr Leu Thr Phe 35 40 45 Ala Pro Glu Thr Ser Arg Tyr Cys Gly Val Leu Ile Arg Cys Gly Arg 50 55 60 Glu Cys Arg Pro Ile Glu Ile Thr His Asn Asn Lys Thr Trp Asn Asn 65 70 75 80 Thr Leu Phe Thr Thr Trp Ser Pro Gly Asp Pro Gln Trp Tyr Thr Val 85 90 95 Ser Val Arg Gly Pro Asp Gly Ser Val Arg Ile Ala Asn Asn Thr Phe 100 105 110 Ile Phe Ala Glu Met Cys Asp Met Val Met Phe Met Ser Arg Gln Tyr 115 120 125 Asn Leu Trp Pro Pro Ser Glu Glu Asn Ile Val Ala Phe Ser Ile Ala 130 135 140 Tyr Cys Leu Cys Thr Cys Leu Ile Thr Ala Ile Leu Cys Ala Cys Leu 145 150 155 160 His Leu Leu Ile Ala Ile Arg Ser Arg Asn Asn Glu Glu Lys Glu Lys 165 170 175 Met Pro <210> 119 <211> 204 <212> PRT <213> Simian adenovirus 32 <400> 119 Met Thr Ser Val Ala Val Ile Phe Thr Ile Ile Thr Gly Phe Thr Thr 1 5 10 15 Ala Val His Gly Met Lys Asn Val Lys Leu Thr Val Tyr Thr Asn Thr 20 25 30 Asn Gln Thr Leu Glu Gly Pro Lys Gly Thr Val Ser Trp Tyr Trp Tyr 35 40 45 Gln Asn Phe Gly Asp Leu Ser Val Trp Leu Cys Asp Gly Thr Thr Ile 50 55 60 Asn Lys Thr Ile Asp Leu Ile Lys Tyr Ser Cys Asp Ser Asp Leu Thr 65 70 75 80 Leu Ile Asn Ile Asn Ala His Tyr Glu Gly Tyr Tyr Tyr Gly Thr Asp 85 90 95 Ile Asn Asp Val Asn Phe Tyr Asn Ile Tyr Val Ser Asp Pro Thr Thr 100 105 110 Ile Pro Thr Lys Pro Ser Thr His Thr Lys Thr Tyr Thr Lys Thr Ser 115 120 125 Thr His Thr Ser Ile Asn Glu Leu Gln Phe Leu Lys Ala Asn Ile Thr 130 135 140 Tyr Asn Ser Thr Ile Ser Pro Thr Ile Pro Asn Glu Thr Asn Ile Pro 145 150 155 160 Asn Ser Met Ile Gly Ile Ile Ala Ala Val Ala Ile Gly Met Ala Ile 165 170 175 Ile Ile Ile Cys Met Ile Val Tyr Ala Cys Cys Tyr Lys Lys Leu Gln 180 185 190 Glu Glu Lys Leu Asp Pro Leu Leu Ser Phe Asp Phe 195 200 <210> 120 <211> 269 <212> PRT <213> Simian adenovirus 32 <400> 120 Met Leu Leu His Thr Leu Ala Phe Ile Ser Ile Phe Gly Phe Ser Leu 1 5 10 15 Gly Gly Lys Ile His Lys Asn Val Thr Val Leu Glu Gly Ala Pro Asn 20 25 30 Ile Thr Leu Gln Gly Val Tyr Val Pro Pro Ser Gln Lys Arg Ser Thr 35 40 45 Ile Asn Ile Thr Trp Glu Thr Val Ile Asn Gly Ser Arg Pro Asn Val 50 55 60 Cys Ala Leu Asn Leu Thr Lys Phe Lys Cys Glu Gly Phe Asp Leu Thr 65 70 75 80 Ile Phe Asn Leu Thr Lys Gln Asp Ser Lys Asn Tyr Phe Gly Glu Ser 85 90 95 Ile Thr Val Leu Thr Ser Gly Tyr Gln Lys Asn Tyr Ile Thr His Asn 100 105 110 Tyr Ala Val Tyr His Val Ile Val Ile Ser Pro Thr Thr His Ala Pro 115 120 125 Ser Thr Thr Gln Val Thr Thr Ala His Ser Asn Thr Tyr Thr His Val 130 135 140 Lys Val Leu Lys Glu Thr Tyr Ser Thr Thr Asn Val Gln Thr Thr Ile 145 150 155 160 Ile Thr Lys Ile Pro Thr Thr Ala Thr Ser Phe Ala Leu His Lys Ser 165 170 175 Ala Leu Ser Cys Ala Pro Thr Leu Thr Thr Leu His Ala Thr Lys Pro 180 185 190 Phe Thr Asn Ile Ser Thr Pro Leu Lys Gln Phe Asp Arg Thr Met Lys 195 200 205 Ile Glu Ile Thr Phe Leu Ile Val Ile Gly Ile Ile Ile Ile Ala Ile 210 215 220 Leu Leu Tyr Tyr Ile Phe Cys Arg Gln Ile Pro Asn Ala Gln Arg Arg 225 230 235 240 Pro Ile Tyr Arg Pro Ile Ile Gly Glu Pro Gln Gln Leu Gln Val Glu 245 250 255 Gly Gly Leu Arg Asn Leu Leu Phe Ser Phe Thr Val Trp 260 265 <210> 121 <211> 91 <212> PRT <213> Simian adenovirus 32 <400> 121 Met Ile Pro Arg Asn Phe Leu Phe Thr Ile Leu Ile Cys Ala Phe Asn 1 5 10 15 Val Cys Ala Thr Phe Ala Thr Val Ala Asn Val Thr Pro Asp Cys Ile 20 25 30 Gly Ala Phe Ala Ser Tyr Val Leu Phe Ala Phe Ile Thr Cys Ile Cys 35 40 45 Val Cys Ser Ile Val Cys Leu Val Ile Asn Phe Phe Gln Leu Val Asp 50 55 60 Trp Val Phe Val Arg Val Ala Tyr Leu Arg His His Pro Glu Tyr Arg 65 70 75 80 Asn Gln Asn Val Ala Ala Ile Leu Arg Leu Ile 85 90 <210> 122 <211> 134 <212> PRT <213> Simian adenovirus 32 <400> 122 Met Thr Asp Pro Leu Ala Ala Ser Ala Ala Glu Glu Leu Leu Asp Met 1 5 10 15 Asp Gly Arg Ala Ser Glu Gln Arg Leu Ala His Leu Arg Ile Arg Gln 20 25 30 Gln Gln Glu Arg Ala Val Lys Glu Leu Arg Asp Ala Ile Glu Ile His 35 40 45 Gln Cys Lys Lys Gly Ile Phe Cys Leu Val Lys Gln Ala Lys Ile Ser 50 55 60 Tyr Glu Ile Thr Ala Asn Asp His Arg Leu Ser Tyr Glu Leu Gly Leu 65 70 75 80 Gln Arg Gln Lys Phe Thr Cys Met Val Gly Ile Asn Pro Ile Val Ile 85 90 95 Thr Gln Gln Ala Gly Asp Thr Lys Gly Cys Ile His Cys Ser Cys Glu 100 105 110 Ser Thr Glu Cys Ile Tyr Thr Leu Leu Lys Thr Leu Cys Gly Leu Arg 115 120 125 Asp Leu Leu Pro Met Asn 130 <210> 123 <211> 319 <212> PRT <213> Simian adenovirus 32 <400> 123 Met Ala Lys Arg Ala Arg Leu Ser Ser Ser Phe Asn Pro Val Tyr Pro 1 5 10 15 Tyr Glu Asp Glu Ser Ser Ser Gln His Pro Phe Ile Asn Pro Gly Phe 20 25 30 Ile Ser Pro Asn Gly Phe Thr Gln Ser Pro Asp Gly Ala Leu Thr Leu 35 40 45 Lys Cys Val Ala Pro Leu Thr Thr Thr Ser Gly Ala Leu Asp Ile Lys 50 55 60 Val Gly Gly Gly Leu Lys Val Asp Ser Thr Asp Gly Ser Leu Glu Glu 65 70 75 80 Asn Ile Gly Thr Thr Glu Pro Leu Asn Lys Ser Asn His Ser Ile Gly 85 90 95 Leu Ala Val Gly Asn Gly Leu Gln Thr Asn Glu Ser Lys Leu Cys Ala 100 105 110 Lys Leu Gly Asp Gly Leu Ile Phe Asp Ser Ser Asn Ala Ile Ala Ile 115 120 125 Lys Asn Asn Thr Leu Trp Thr Gly Ala Lys Pro Glu Ala Asn Cys Ile 130 135 140 Ile Glu Tyr Gly Lys Glu Ser Thr Asp Ser Lys Leu Thr Leu Val Leu 145 150 155 160 Val Lys Asn Gly Gly Ile Val Asn Gly Tyr Val Thr Leu Met Gly Ala 165 170 175 Ser Asp Tyr Val Asn Thr Leu Phe Thr Asn Lys Tyr Ala Ser Ile Asn 180 185 190 Val Glu Leu Tyr Phe Asp Ala Asn Gly His Leu Leu Thr Asp Ser Ser 195 200 205 Ser Leu Lys Thr Asp Leu Gln Leu Lys Ser Gln Thr Thr Glu Ser Ser 210 215 220 Thr Lys Gly Phe Met Pro Ser Thr Ile Ala Tyr Pro Phe Val Leu Pro 225 230 235 240 Asn Ala Gly Arg Asp Asn Glu Asp Tyr Ile Tyr Gly Gln Cys Tyr Tyr 245 250 255 Lys Ala Ser Ser Asp Gly Thr Leu Phe Pro Leu Glu Val Thr Val Met 260 265 270 Leu Asn Lys Arg Leu Pro Asp Ser Arg Thr Ser Tyr Val Met Thr Phe 275 280 285 Ser Trp Ser Leu Asn Ala Thr Gln Ala Pro Glu Thr Thr Gln Ala Thr 290 295 300 Leu Ile Thr Ser Pro Phe Phe Phe Ser Tyr Ile Arg Glu Asp Asp 305 310 315 <210> 124 <211> 550 <212> DNA <213> Simian adenovirus 32 <220> <221> CDS <222> (4)..(546) <223> label=Elb\19K <400> 124 tcc atg gag gtt tgg gct atc ttg gaa gat ctc agg cag act aga caa 48 Met Glu Val Trp Ala Ile Leu Glu Asp Leu Arg Gln Thr Arg Gln 1 5 10 15 ctg cta gaa aac gcc tcg gac gga gtc tct agt ctt tgg aga ttc tgg 96 Leu Leu Glu Asn Ala Ser Asp Gly Val Ser Ser Leu Trp Arg Phe Trp 20 25 30 ttc ggt ggt gat cta gct agg cta gtc ttt agg gta aaa cgg gag tat 144 Phe Gly Gly Asp Leu Ala Arg Leu Val Phe Arg Val Lys Arg Glu Tyr 35 40 45 agt gaa gaa ttt gaa aag tta ttg gaa gac agt cca gga ctt ttt gaa 192 Ser Glu Glu Phe Glu Lys Leu Leu Glu Asp Ser Pro Gly Leu Phe Glu 50 55 60 gcc ctt aac ttg ggc cac cag gct cat ttt aag gag aag gtt tta tca 240 Ala Leu Asn Leu Gly His Gln Ala His Phe Lys Glu Lys Val Leu Ser 65 70 75 gtt tta gat ttt tct acc cct ggt aga act gct gct gct gta gct ttc 288 Val Leu Asp Phe Ser Thr Pro Gly Arg Thr Ala Ala Ala Val Ala Phe 80 85 90 95 ctt act ttt ata ttg gat aaa tgg atc cca caa acc cac ttc agc aag 336 Leu Thr Phe Ile Leu Asp Lys Trp Ile Pro Gln Thr His Phe Ser Lys 100 105 110 gga tac gtc ttg gat ttc ata gca gca gct ttg tgg aga aca tgg aag 384 Gly Tyr Val Leu Asp Phe Ile Ala Ala Ala Leu Trp Arg Thr Trp Lys 115 120 125 gcc cgc agg ctg agg ata atc tta gat tac tgg cca gtg cag cct ctg 432 Ala Arg Arg Leu Arg Ile Ile Leu Asp Tyr Trp Pro Val Gln Pro Leu 130 135 140 ggc gta gca gca atc ctg aga cac cca ccg gcc atg cca gcg gtt ttg 480 Gly Val Ala Ala Ile Leu Arg His Pro Pro Ala Met Pro Ala Val Leu 145 150 155 gag gag gag cag cag gag gac aac ccg aga gcc ggc ctg gac cct ccg 528 Glu Glu Glu Gln Gln Glu Asp Asn Pro Arg Ala Gly Leu Asp Pro Pro 160 165 170 175 gtg gag gag gcg gag gag tagc 550 Val Glu Glu Ala Glu Glu 180 <210> 125 <211> 181 <212> PRT <213> Simian adenovirus 32 <400> 125 Met Glu Val Trp Ala Ile Leu Glu Asp Leu Arg Gln Thr Arg Gln Leu 1 5 10 15 Leu Glu Asn Ala Ser Asp Gly Val Ser Ser Leu Trp Arg Phe Trp Phe 20 25 30 Gly Gly Asp Leu Ala Arg Leu Val Phe Arg Val Lys Arg Glu Tyr Ser 35 40 45 Glu Glu Phe Glu Lys Leu Leu Glu Asp Ser Pro Gly Leu Phe Glu Ala 50 55 60 Leu Asn Leu Gly His Gln Ala His Phe Lys Glu Lys Val Leu Ser Val 65 70 75 80 Leu Asp Phe Ser Thr Pro Gly Arg Thr Ala Ala Ala Val Ala Phe Leu 85 90 95 Thr Phe Ile Leu Asp Lys Trp Ile Pro Gln Thr His Phe Ser Lys Gly 100 105 110 Tyr Val Leu Asp Phe Ile Ala Ala Ala Leu Trp Arg Thr Trp Lys Ala 115 120 125 Arg Arg Leu Arg Ile Ile Leu Asp Tyr Trp Pro Val Gln Pro Leu Gly 130 135 140 Val Ala Ala Ile Leu Arg His Pro Pro Ala Met Pro Ala Val Leu Glu 145 150 155 160 Glu Glu Gln Gln Glu Asp Asn Pro Arg Ala Gly Leu Asp Pro Pro Val 165 170 175 Glu Glu Ala Glu Glu 180 <210> 126 <211> 5030 <212> DNA <213> Simian adenovirus 32 <220> <221> CDS <222> (1)..(615) <223> label=22K <220> <221> CDS <222> (1909)..(2352) <223> label=E3\CR1\alpha <220> <221> CDS <222> (4603)..(5025) <223> label=E3\RID\beta <400> 126 atg tcc cag cgc cga gga agc aag aag ttg aag gtg cag ctg ccg ccc 48 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 cca gag gat atg gag gaa gac tgg gac agt cag gca gag gag gag gag 96 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu Glu Glu Glu 20 25 30 atg gaa gat tgg gac agc cag gca gag gag gcg gac agc ctg gag gaa 144 Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Ala Asp Ser Leu Glu Glu 35 40 45 gac agt ttg gag gag gaa gac gag gag gca gag gag gtg gaa gaa gca 192 Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu Ala 50 55 60 acc gcc gcc aaa cag ttg tcc tcg aca gcg gag aca agc agg gcc cca 240 Thr Ala Ala Lys Gln Leu Ser Ser Thr Ala Glu Thr Ser Arg Ala Pro 65 70 75 80 gac agc agc agc agc acg gct aca atc tcc gct ccg ggt cgg ggg gcc 288 Asp Ser Ser Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Arg Gly Ala 85 90 95 cag cgg cgt ccc aac agt aga tgg gac gag acc ggg cga ttc ccg aac 336 Gln Arg Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe Pro Asn 100 105 110 ccg acc acc gct tcc aag acc ggt aag aag gag cgg cag gga tac aag 384 Pro Thr Thr Ala Ser Lys Thr Gly Lys Lys Glu Arg Gln Gly Tyr Lys 115 120 125 tcc tgg cgg ggg cat aag aat gcc atc atc tcc tgc ttg cat gaa tgt 432 Ser Trp Arg Gly His Lys Asn Ala Ile Ile Ser Cys Leu His Glu Cys 130 135 140 ggg ggc aac ata tcc ttc acc cgg cgc tac ctg ctc ttc cac cac ggg 480 Gly Gly Asn Ile Ser Phe Thr Arg Arg Tyr Leu Leu Phe His His Gly 145 150 155 160 gtg aac ttc ccc cgc aat gtc ttg cat tac tac cgt cac ctc cac agc 528 Val Asn Phe Pro Arg Asn Val Leu His Tyr Tyr Arg His Leu His Ser 165 170 175 ccc tac tac agc cag caa gtc cca aca gcc tcg gca gag aaa aac agc 576 Pro Tyr Tyr Ser Gln Gln Val Pro Thr Ala Ser Ala Glu Lys Asn Ser 180 185 190 agc agc ggg gac ctc cag cag aaa acc agc agc agc agt tagaaagtcc 625 Ser Ser Gly Asp Leu Gln Gln Lys Thr Ser Ser Ser Ser 195 200 205 agtgcagcag gaggaggact gaggatcaca gcgaacgagc cagcgcagac ccgagagctg 685 agaaacagga tctttccaac cctctatgcc atcttccagc agagtcgggg gcaagagcag 745 gaactgaaag taaaaaaccg atctctgcgc tcgctcaccc gaagttgttt gtatcacaag 805 agcgaagacc aacttcagcg cactctcgag gacgccgagg ctctcttcaa caagtactgc 865 gcgctgactc ttaaagagta gcccgcgccc gcgctcgctc gaaaaaggcg ggaattacgt 925 cacccttggc acctgtcctt tgccccgtca tgagtaaaga aattcccacg ccttacatgt 985 ggagctatca gccccaaatg ggactggcag caggcgcctc ccaggactac tccacccgca 1045 tgaattggct cagcgccggg ccctcgatga tctcacgggt taatgatata cgagcttacc 1105 gaaaccagtt actcctagaa cagtcagctc tcaccaccac accccgccaa caccttaatc 1165 cccggaattg gcccgccgcc ctggtgtacc aggaaacccc cgctcccacc accgtactac 1225 ttcctcgaga cgcccaggcc gaagttcaga tgactaacgc aggtgtacag ctggcgggcg 1285 gttccgccct gtgtcgtcac cggcctcagc agagtataaa acgcctggtg atcagaggcc 1345 gaggtatcca gctcaacgac gagtcggtga gctcttcgct tggtctgcga ccagacggag 1405 tcttccaaat cgccggctgt gggagatctt ccttcactcc tcgtcaggct gtcctgactt 1465 tggagagttc gtcctcgcag ccccgctcgg gcggcatcgg gactctccag tttgtggagg 1525 agtttactcc ctctgtctac ttcaacccct tctccggctc tcctggccag tacccggacg 1585 agttcatacc gaacttcgac gcaatcagcg agtcagtgga tggctatgat tgatgtctgg 1645 tggcgcggct gagttagctc gactgcgaca tctagaccac tgccgccgct ttcgctgttt 1705 cgcccgggaa ctcaccgagt tcatctactt cgaactcccc gaggagcacc ctcagggacc 1765 ggcccacgga gtgcggatta ccatcgaagg gggaatagac tctcgcctgc atcggatctt 1825 ctgccagcga cctgtgctga ttgagcgcga ccagggaact acaacagtct ccatctactg 1885 catctgtaac caccccggat tgc atg aaa gcc ttt gct gtc tta ttt gtg ctg 1938 Met Lys Ala Phe Ala Val Leu Phe Val Leu 210 215 agt tta ata aaa act gag tta aga ctc acc ttc gga cta ccg ctt ctt 1986 Ser Leu Ile Lys Thr Glu Leu Arg Leu Thr Phe Gly Leu Pro Leu Leu 220 225 230 caa ccc gga cct tac aac acc agc cag acc ctc cgt tcc agc cag aag 2034 Gln Pro Gly Pro Tyr Asn Thr Ser Gln Thr Leu Arg Ser Ser Gln Lys 235 240 245 aac cag acc ctt cct cta atc cag gac tct aat tct acc tcc cca gcg 2082 Asn Gln Thr Leu Pro Leu Ile Gln Asp Ser Asn Ser Thr Ser Pro Ala 250 255 260 cct ttt cct act aac ctt ccc gat act aac aac ctc gga gct cag ctg 2130 Pro Phe Pro Thr Asn Leu Pro Asp Thr Asn Asn Leu Gly Ala Gln Leu 265 270 275 caa cac cgc ttc tcc aga agc ctc ctt tct gcc aat act act act ccc 2178 Gln His Arg Phe Ser Arg Ser Leu Leu Ser Ala Asn Thr Thr Thr Pro 280 285 290 295 aaa acc gga ggt gag ctc cgt ggt ctc cct act gac aac ccc tgg gtg 2226 Lys Thr Gly Gly Glu Leu Arg Gly Leu Pro Thr Asp Asn Pro Trp Val 300 305 310 gta gcg ggt ttt gta gca cta gga gta gtt gcg ggt ggg ctg gtg ctt 2274 Val Ala Gly Phe Val Ala Leu Gly Val Val Ala Gly Gly Leu Val Leu 315 320 325 atc ctc tgc tac cta tac aca cct tgc tgt gct tat tta gta gtc ttg 2322 Ile Leu Cys Tyr Leu Tyr Thr Pro Cys Cys Ala Tyr Leu Val Val Leu 330 335 340 tgc tgt tgg ttt aag aaa tgg ggg tcg tac tagtcgcgct tgctttactt 2372 Cys Cys Trp Phe Lys Lys Trp Gly Ser Tyr 345 350 tcgcttttgg gtctgggctc tactacgcta gcgaatcagc ctttactatt agatcctgat 2432 aatgttgatc catgcctaac atttgatcca gaaaactgca cacttacttt tgcacctgaa 2492 acaagtcgct actgtggagt tcttattagg tgcggacggg aatgcaggcc cattgagatt 2552 acacacaata acaaaacttg gaacaacaca ttattcacca catggtctcc aggagatcct 2612 cagtggtata ctgtctctgt ccggggtcct gacggttccg tccgcatagc taataacact 2672 ttcatttttg ctgaaatgtg cgatatggtc atgttcatga gcagacagta taacctatgg 2732 cctcccagcg aggaaaacat tgtggcattc tccattgctt attgcttatg tacttgcctt 2792 atcactgcta tcttgtgtgc gtgcttgcat ttgcttattg ctattcgctc cagaaacaat 2852 gaggaaaaag aaaaaatgcc ttaacctttt tcctcatacc ttttttacag catgacttct 2912 gtcgcagtca tttttactat tattaccggc tttactactg ccgtgcatgg aatgaaaaat 2972 gttaaactaa ctgtctatac taacaccaac caaacactgg aggggcctaa agggacagtt 3032 tcatggtatt ggtatcaaaa ttttggcgat ctgtctgtat ggttgtgcga tggaacaacc 3092 attaataaga ccattgattt aattaaatac agttgcgatt cagatttaac actaatcaac 3152 attaatgctc attatgaagg ttactattat ggaactgaca taaatgatgt aaacttctac 3212 aacatttatg tatcagaccc aacaaccatt ccaactaaac cctccacaca cactaaaact 3272 tacactaaaa cttccacaca cacaagcatc aatgagttac aatttctaaa agctaacata 3332 acatacaatt ctaccatctc gcctactatt cccaatgaaa caaatattcc taattcaatg 3392 attggaatta ttgctgcagt tgctattgga atggcgatca taataatatg tatgatcgtt 3452 tatgcttgct gctataaaaa acttcaagaa gaaaaattag atccactact aagctttgat 3512 ttttaaattt ttttttgtag aaacatgctt cttcatactt tagcttttat ttccattttt 3572 ggtttctcac ttggaggtaa aatacataag aatgttaccg tgttagaggg cgctccaaat 3632 ataacactcc aaggagttta tgtcccaccc agtcaaaaaa gaagcactat taacataact 3692 tgggaaactg taataaatgg aagcagacca aatgtatgtg ctttaaattt aacaaaattc 3752 aaatgtgagg gttttgatct tactatcttt aatttaacta aacaagactc caaaaattat 3812 tttggtgaaa gtataactgt cttaacttct ggttatcaaa aaaattatat aacccacaat 3872 tatgcagttt atcatgttat tgttatatct ccaactactc atgcgccctc taccacacaa 3932 gtaactacag ctcattctaa cacttacacc catgtaaagg tattaaaaga aacatacagt 3992 actaccaatg tgcagactac tataatcaca aaaataccca caacagctac ctcatttgct 4052 ttacacaaat cagcactttc atgcgcccca acgctcacca ctttgcatgc tactaaacct 4112 tttactaata tatctactcc cttaaaacaa tttgatcgaa ctatgaaaat agaaattacc 4172 tttcttattg tcataggaat aattatcatt gcaatcttgc tttactacat attctgtcgc 4232 caaatcccca atgctcaaag acgacctata tatagaccta tcataggtga accccaacaa 4292 cttcaagtgg agggaggctt aagaaatctt ctgttctctt ttacagtatg gtgatcaaca 4352 atcatgatcc ctagaaattt cctcttcacc atactcatct gtgcttttaa tgtttgtgct 4412 actttcgcca cagttgccaa tgtcactcca gactgtatag gagcatttgc ctcctatgtg 4472 cttttcgcat ttattacctg catctgtgtt tgtagcatag tttgcctggt tattaatttc 4532 tttcaacttg tagactgggt ttttgtacgc gttgcctacc tgcggcatca ccctgaatac 4592 cgcaaccaaa atg ttg cag caa ttc tta ggc tca ttt aaa acc atg caa 4641 Met Leu Gln Gln Phe Leu Gly Ser Phe Lys Thr Met Gln 355 360 365 act ctg cta cta ctt ctg cta gtt ata cat cca tgt gcc tcc tta aac 4689 Thr Leu Leu Leu Leu Leu Leu Val Ile His Pro Cys Ala Ser Leu Asn 370 375 380 ccc aca agc ccc aca aaa tta cac cta aga aaa tgt aaa ttt caa gaa 4737 Pro Thr Ser Pro Thr Lys Leu His Leu Arg Lys Cys Lys Phe Gln Glu 385 390 395 cca tgg aaa ttc ctt gaa tgc tat cat gaa aca tct gat ttc ccc aca 4785 Pro Trp Lys Phe Leu Glu Cys Tyr His Glu Thr Ser Asp Phe Pro Thr 400 405 410 tac tgg att aca atc att ggg att gtt aat cta gtt tct tgc aca cta 4833 Tyr Trp Ile Thr Ile Ile Gly Ile Val Asn Leu Val Ser Cys Thr Leu 415 420 425 430 ttc tct ttc ctt gtt tac cac tta ttt gat ttt gga tgg aat gcc ctc 4881 Phe Ser Phe Leu Val Tyr His Leu Phe Asp Phe Gly Trp Asn Ala Leu 435 440 445 aat gca ctc act tac cca caa gaa cca gag gaa cat ata cca cta caa 4929 Asn Ala Leu Thr Tyr Pro Gln Glu Pro Glu Glu His Ile Pro Leu Gln 450 455 460 aac atg caa cca cta gca cta gta gaa tat gaa aat gaa cca cag ccc 4977 Asn Met Gln Pro Leu Ala Leu Val Glu Tyr Glu Asn Glu Pro Gln Pro 465 470 475 ccg atg ctc cct gct att agt tac ttc aac cta act gga gga gat gac 5025 Pro Met Leu Pro Ala Ile Ser Tyr Phe Asn Leu Thr Gly Gly Asp Asp 480 485 490 tgacc 5030 <210> 127 <211> 205 <212> PRT <213> Simian adenovirus 32 <400> 127 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu Glu Glu Glu 20 25 30 Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Ala Asp Ser Leu Glu Glu 35 40 45 Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu Ala 50 55 60 Thr Ala Ala Lys Gln Leu Ser Ser Thr Ala Glu Thr Ser Arg Ala Pro 65 70 75 80 Asp Ser Ser Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Arg Gly Ala 85 90 95 Gln Arg Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe Pro Asn 100 105 110 Pro Thr Thr Ala Ser Lys Thr Gly Lys Lys Glu Arg Gln Gly Tyr Lys 115 120 125 Ser Trp Arg Gly His Lys Asn Ala Ile Ile Ser Cys Leu His Glu Cys 130 135 140 Gly Gly Asn Ile Ser Phe Thr Arg Arg Tyr Leu Leu Phe His His Gly 145 150 155 160 Val Asn Phe Pro Arg Asn Val Leu His Tyr Tyr Arg His Leu His Ser 165 170 175 Pro Tyr Tyr Ser Gln Gln Val Pro Thr Ala Ser Ala Glu Lys Asn Ser 180 185 190 Ser Ser Gly Asp Leu Gln Gln Lys Thr Ser Ser Ser Ser 195 200 205 <210> 128 <211> 148 <212> PRT <213> Simian adenovirus 32 <400> 128 Met Lys Ala Phe Ala Val Leu Phe Val Leu Ser Leu Ile Lys Thr Glu 1 5 10 15 Leu Arg Leu Thr Phe Gly Leu Pro Leu Leu Gln Pro Gly Pro Tyr Asn 20 25 30 Thr Ser Gln Thr Leu Arg Ser Ser Gln Lys Asn Gln Thr Leu Pro Leu 35 40 45 Ile Gln Asp Ser Asn Ser Thr Ser Pro Ala Pro Phe Pro Thr Asn Leu 50 55 60 Pro Asp Thr Asn Asn Leu Gly Ala Gln Leu Gln His Arg Phe Ser Arg 65 70 75 80 Ser Leu Leu Ser Ala Asn Thr Thr Thr Pro Lys Thr Gly Gly Glu Leu 85 90 95 Arg Gly Leu Pro Thr Asp Asn Pro Trp Val Val Ala Gly Phe Val Ala 100 105 110 Leu Gly Val Val Ala Gly Gly Leu Val Leu Ile Leu Cys Tyr Leu Tyr 115 120 125 Thr Pro Cys Cys Ala Tyr Leu Val Val Leu Cys Cys Trp Phe Lys Lys 130 135 140 Trp Gly Ser Tyr 145 <210> 129 <211> 141 <212> PRT <213> Simian adenovirus 32 <400> 129 Met Leu Gln Gln Phe Leu Gly Ser Phe Lys Thr Met Gln Thr Leu Leu 1 5 10 15 Leu Leu Leu Leu Val Ile His Pro Cys Ala Ser Leu Asn Pro Thr Ser 20 25 30 Pro Thr Lys Leu His Leu Arg Lys Cys Lys Phe Gln Glu Pro Trp Lys 35 40 45 Phe Leu Glu Cys Tyr His Glu Thr Ser Asp Phe Pro Thr Tyr Trp Ile 50 55 60 Thr Ile Ile Gly Ile Val Asn Leu Val Ser Cys Thr Leu Phe Ser Phe 65 70 75 80 Leu Val Tyr His Leu Phe Asp Phe Gly Trp Asn Ala Leu Asn Ala Leu 85 90 95 Thr Tyr Pro Gln Glu Pro Glu Glu His Ile Pro Leu Gln Asn Met Gln 100 105 110 Pro Leu Ala Leu Val Glu Tyr Glu Asn Glu Pro Gln Pro Pro Met Leu 115 120 125 Pro Ala Ile Ser Tyr Phe Asn Leu Thr Gly Gly Asp Asp 130 135 140 <210> 130 <211> 880 <212> DNA <213> Simian adenovirus 32 <220> <221> CDS <222> (3)..(579) <223> label=Ela <220> <221> CDS <222> (668)..(873) <223> label=Ela <400> 130 aa atg aga cac ctg cga ttc ctg cct cag gaa atc tcc atc gag acc 47 Met Arg His Leu Arg Phe Leu Pro Gln Glu Ile Ser Ile Glu Thr 1 5 10 15 ggg aat gaa ata cta cag ctt gtg gta aat gcc ctg atg gga gac gat 95 Gly Asn Glu Ile Leu Gln Leu Val Val Asn Ala Leu Met Gly Asp Asp 20 25 30 ccg gag ccg cct gcg cat ccg ttc gat cct cct acg ctt cat gaa ctg 143 Pro Glu Pro Pro Ala His Pro Phe Asp Pro Pro Thr Leu His Glu Leu 35 40 45 tat gat tta gag gta gac ggg ccg gag gat cct aac gag gaa gct gtg 191 Tyr Asp Leu Glu Val Asp Gly Pro Glu Asp Pro Asn Glu Glu Ala Val 50 55 60 aat ggt ttt ttt agc gaa tct atg cta ttg gct gct aat gaa gga gtg 239 Asn Gly Phe Phe Ser Glu Ser Met Leu Leu Ala Ala Asn Glu Gly Val 65 70 75 gac ata gac cca ccg tcg gag acc ctc gat acc cca ggg gtg att gtg 287 Asp Ile Asp Pro Pro Ser Glu Thr Leu Asp Thr Pro Gly Val Ile Val 80 85 90 95 gag agc ggc aga ggt ggg aaa aca ttg cct gaa ctt ggt gct gct gaa 335 Glu Ser Gly Arg Gly Gly Lys Thr Leu Pro Glu Leu Gly Ala Ala Glu 100 105 110 atg gac ttg cgc tgt tat gaa gag ggc ttt cct ccg agt gat gat gaa 383 Met Asp Leu Arg Cys Tyr Glu Glu Gly Phe Pro Pro Ser Asp Asp Glu 115 120 125 gag gag gaa aat gtg cag tcg atc cag acc gca gcg ggt gag gga atg 431 Glu Glu Glu Asn Val Gln Ser Ile Gln Thr Ala Ala Gly Glu Gly Met 130 135 140 aaa gct gcc aat gat ggt ttt aag ttg gac tgc ccg gag ctg cct gga 479 Lys Ala Ala Asn Asp Gly Phe Lys Leu Asp Cys Pro Glu Leu Pro Gly 145 150 155 cat ggc tgt aag tct tgt gaa ttt cac agg aat agt act gga cta aaa 527 His Gly Cys Lys Ser Cys Glu Phe His Arg Asn Ser Thr Gly Leu Lys 160 165 170 175 gaa ctg ttg tgc tcg ctt tgc tat atg aga acg cac tgc cat ttt att 575 Glu Leu Leu Cys Ser Leu Cys Tyr Met Arg Thr His Cys His Phe Ile 180 185 190 tac a gtaagtgtgt ctaacttaaa tttaaaggga cagtgtagca gtttagtgtc 629 Tyr tgttgaatgt gggatttatg tctttgtgat ttttatag gt cct gtg tct gat gct 684 Ser Pro Val Ser Asp Ala 195 gat gaa tcg cct tct cct gat tca act acc tca cct cct gaa att cag 732 Asp Glu Ser Pro Ser Pro Asp Ser Thr Thr Ser Pro Pro Glu Ile Gln 200 205 210 gcg cca gtc cct gca aac gta tgc aag ccc att cct gtg aag gct aag 780 Ala Pro Val Pro Ala Asn Val Cys Lys Pro Ile Pro Val Lys Ala Lys 215 220 225 230 cct ggg aaa cgc cct gct gtg gat aaa ctg gag gac ttg ctt gag ggt 828 Pro Gly Lys Arg Pro Ala Val Asp Lys Leu Glu Asp Leu Leu Glu Gly 235 240 245 ggg gat gga cct ttg gac ttg agt acc cgg aaa ctg cca agg caa 873 Gly Asp Gly Pro Leu Asp Leu Ser Thr Arg Lys Leu Pro Arg Gln 250 255 260 tgagtgc 880 <210> 131 <211> 261 <212> PRT <213> Simian adenovirus 32 <400> 131 Met Arg His Leu Arg Phe Leu Pro Gln Glu Ile Ser Ile Glu Thr Gly 1 5 10 15 Asn Glu Ile Leu Gln Leu Val Val Asn Ala Leu Met Gly Asp Asp Pro 20 25 30 Glu Pro Pro Ala His Pro Phe Asp Pro Pro Thr Leu His Glu Leu Tyr 35 40 45 Asp Leu Glu Val Asp Gly Pro Glu Asp Pro Asn Glu Glu Ala Val Asn 50 55 60 Gly Phe Phe Ser Glu Ser Met Leu Leu Ala Ala Asn Glu Gly Val Asp 65 70 75 80 Ile Asp Pro Pro Ser Glu Thr Leu Asp Thr Pro Gly Val Ile Val Glu 85 90 95 Ser Gly Arg Gly Gly Lys Thr Leu Pro Glu Leu Gly Ala Ala Glu Met 100 105 110 Asp Leu Arg Cys Tyr Glu Glu Gly Phe Pro Pro Ser Asp Asp Glu Glu 115 120 125 Glu Glu Asn Val Gln Ser Ile Gln Thr Ala Ala Gly Glu Gly Met Lys 130 135 140 Ala Ala Asn Asp Gly Phe Lys Leu Asp Cys Pro Glu Leu Pro Gly His 145 150 155 160 Gly Cys Lys Ser Cys Glu Phe His Arg Asn Ser Thr Gly Leu Lys Glu 165 170 175 Leu Leu Cys Ser Leu Cys Tyr Met Arg Thr His Cys His Phe Ile Tyr 180 185 190 Ser Pro Val Ser Asp Ala Asp Glu Ser Pro Ser Pro Asp Ser Thr Thr 195 200 205 Ser Pro Pro Glu Ile Gln Ala Pro Val Pro Ala Asn Val Cys Lys Pro 210 215 220 Ile Pro Val Lys Ala Lys Pro Gly Lys Arg Pro Ala Val Asp Lys Leu 225 230 235 240 Glu Asp Leu Leu Glu Gly Gly Asp Gly Pro Leu Asp Leu Ser Thr Arg 245 250 255 Lys Leu Pro Arg Gln 260 <210> 132 <211> 890 <212> DNA <213> Simian adenovirus 32 <220> <221> CDS <222> (1)..(358) <223> label=33K <220> <221> CDS <222> (528)..(883) <223> label=33K <400> 132 atg tcc cag cgc cga gga agc aag aag ttg aag gtg cag ctg ccg ccc 48 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 cca gag gat atg gag gaa gac tgg gac agt cag gca gag gag gag gag 96 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu Glu Glu Glu 20 25 30 atg gaa gat tgg gac agc cag gca gag gag gcg gac agc ctg gag gaa 144 Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Ala Asp Ser Leu Glu Glu 35 40 45 gac agt ttg gag gag gaa gac gag gag gca gag gag gtg gaa gaa gca 192 Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu Ala 50 55 60 acc gcc gcc aaa cag ttg tcc tcg aca gcg gag aca agc agg gcc cca 240 Thr Ala Ala Lys Gln Leu Ser Ser Thr Ala Glu Thr Ser Arg Ala Pro 65 70 75 80 gac agc agc agc agc acg gct aca atc tcc gct ccg ggt cgg ggg gcc 288 Asp Ser Ser Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Arg Gly Ala 85 90 95 cag cgg cgt ccc aac agt aga tgg gac gag acc ggg cga ttc ccg aac 336 Gln Arg Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe Pro Asn 100 105 110 ccg acc acc gct tcc aag acc g gtaagaagga gcggcaggga tacaagtcct 388 Pro Thr Thr Ala Ser Lys Thr 115 ggcgggggca taagaatgcc atcatctcct gcttgcatga atgtgggggc aacatatcct 448 tcacccggcg ctacctgctc ttccaccacg gggtgaactt cccccgcaat gtcttgcatt 508 actaccgtca cctccacag cc cct act aca gcc agc aag tcc caa cag cct 559 Ala Pro Thr Thr Ala Ser Lys Ser Gln Gln Pro 125 130 cgg cag aga aaa aca gca gca gcg ggg acc tcc agc aga aaa cca gca 607 Arg Gln Arg Lys Thr Ala Ala Ala Gly Thr Ser Ser Arg Lys Pro Ala 135 140 145 gca gca gtt aga aag tcc agt gca gca gga gga gga ctg agg atc aca 655 Ala Ala Val Arg Lys Ser Ser Ala Ala Gly Gly Gly Leu Arg Ile Thr 150 155 160 gcg aac gag cca gcg cag acc cga gag ctg aga aac agg atc ttt cca 703 Ala Asn Glu Pro Ala Gln Thr Arg Glu Leu Arg Asn Arg Ile Phe Pro 165 170 175 acc ctc tat gcc atc ttc cag cag agt cgg ggg caa gag cag gaa ctg 751 Thr Leu Tyr Ala Ile Phe Gln Gln Ser Arg Gly Gln Glu Gln Glu Leu 180 185 190 aaa gta aaa aac cga tct ctg cgc tcg ctc acc cga agt tgt ttg tat 799 Lys Val Lys Asn Arg Ser Leu Arg Ser Leu Thr Arg Ser Cys Leu Tyr 195 200 205 210 cac aag agc gaa gac caa ctt cag cgc act ctc gag gac gcc gag gct 847 His Lys Ser Glu Asp Gln Leu Gln Arg Thr Leu Glu Asp Ala Glu Ala 215 220 225 ctc ttc aac aag tac tgc gcg ctg act ctt aaa gag tagcccg 890 Leu Phe Asn Lys Tyr Cys Ala Leu Thr Leu Lys Glu 230 235 <210> 133 <211> 238 <212> PRT <213> Simian adenovirus 32 <400> 133 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu Glu Glu Glu 20 25 30 Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Ala Asp Ser Leu Glu Glu 35 40 45 Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu Ala 50 55 60 Thr Ala Ala Lys Gln Leu Ser Ser Thr Ala Glu Thr Ser Arg Ala Pro 65 70 75 80 Asp Ser Ser Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Arg Gly Ala 85 90 95 Gln Arg Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe Pro Asn 100 105 110 Pro Thr Thr Ala Ser Lys Thr Ala Pro Thr Thr Ala Ser Lys Ser Gln 115 120 125 Gln Pro Arg Gln Arg Lys Thr Ala Ala Ala Gly Thr Ser Ser Arg Lys 130 135 140 Pro Ala Ala Ala Val Arg Lys Ser Ser Ala Ala Gly Gly Gly Leu Arg 145 150 155 160 Ile Thr Ala Asn Glu Pro Ala Gln Thr Arg Glu Leu Arg Asn Arg Ile 165 170 175 Phe Pro Thr Leu Tyr Ala Ile Phe Gln Gln Ser Arg Gly Gln Glu Gln 180 185 190 Glu Leu Lys Val Lys Asn Arg Ser Leu Arg Ser Leu Thr Arg Ser Cys 195 200 205 Leu Tyr His Lys Ser Glu Asp Gln Leu Gln Arg Thr Leu Glu Asp Ala 210 215 220 Glu Ala Leu Phe Asn Lys Tyr Cys Ala Leu Thr Leu Lys Glu 225 230 235 <210> 134 <211> 35694 <212> DNA <213> Simian adenovirus 33 <220> <221> repeat_region <222> (1)..(131) <223> label=ITR <220> <221> CDS <222> (1925)..(3409) <223> label=Elb\55K <220> <221> CDS <222> (3504)..(3917) <223> label=pIX <220> <221> misc_feature <222> (3986)..(5607) <223> complement (3986..5316, 5595..5607) label=IVa2 <220> <221> misc_feature <222> (5089)..(13926) <223> complement (5089..8658, 13918..13926) label=pol <220> <221> misc_feature <222> (8460)..(13926) <223> complement (8460..10439, 13918..13926) label=pTP <220> <221> CDS <222> (10924)..(12093) <223> label=52K <220> <221> CDS <222> (12121)..(13884) <223> label=pIIIa <220> <221> CDS <222> (13972)..(15684) <223> label=penton <220> <221> CDS <222> (15692)..(16264) <223> label=pVII <220> <221> CDS <222> (16309)..(17358) <223> label=V <220> <221> CDS <222> (17390)..(17614) <223> label=pX <220> <221> CDS <222> (17691)..(18440) <223> label=pVI <220> <221> CDS <222> (18565)..(21417) <223> label=hexon <220> <221> CDS <222> (21457)..(22083) <223> label=protease <220> <221> misc_feature <222> (22177)..(23724) <223> complement label=DBP <220> <221> CDS <222> (23755)..(26253) <223> label=100K <220> <221> CDS <222> (26901)..(27581) <223> label=pVIII <220> <221> CDS <222> (27584)..(27901) <223> label=E3\12.5K <220> <221> CDS <222> (28295)..(28810) <223> label=E3\gp19K <220> <221> CDS <222> (29395)..(29958) <223> label=E3\CR1\gamma <220> <221> CDS <222> (29979)..(30335) <223> label=E3\CR1\delta <220> <221> CDS <222> (30380)..(30652) <223> label=E3\RID\alpha <220> <221> CDS <222> (31057)..(31461) <223> label=E3\14.7K <220> <221> CDS <222> (31699)..(32673) <223> label=fiber <220> <221> misc_feature <222> (32721)..(33880) <223> complement (32721..32969, 33683..33880) label=E4\orf6/7 <220> <221> misc_feature <222> (32969)..(33880) <223> complement label=E4\orf6 <220> <221> misc_feature <222> (33771)..(34151) <223> complement label=E4\orf4 <220> <221> misc_feature <222> (34164)..(34514) <223> complement label=E4\orf3 <220> <221> misc_feature <222> (34514)..(34900) <223> complement label=E4\orf2 <220> <221> misc_feature <222> (34945)..(35316) <223> complement label=E4\orf1 <220> <221> repeat_region <222> (35564)..(35694) <223> complement label=ITR <400> 134 catcatcaat aatatacctt ataaatggaa cggtgccaac atgcaaatga gcttttgaaa 60 atggagggcg gaaggggatt ggctacgggt tcaacggtca aagggggcgg gccggcgcgg 120 ggaggtgacg tgatttgtgt gggaggagtt atgttgcaag ttctcgcggt aaaagtgacg 180 caaaacgagg tgtggtttga acacggaagt agacagtttt cccgcgctta ctgacaggat 240 atgaggtagt tttgggcgga tgcaagtgaa aattctccat tttcgcgcga aaactgaatg 300 aggaagtgaa tttctgagta atttcgcgtt tatgacaggg tggagtattt gccgagggcc 360 gagtagactt tgaccgatta cgtggaggtt tcgattaccg tgtttttcac ctaaatttcc 420 gcgtacggtg tcaaagtcct gtgtttttac gtaggtgtca gctgatcgct agggtattta 480 aacctgacga gttccgtcaa gaggccactc ttgagtgcca gcgagaagag ttttctcctc 540 cgcgccgcga gtcagttctg cactttgaaa atgagacacc tgcgattcct gccacaggag 600 attatctcca gcgagaccgg gatcgaaata ctggagttcg tggtaaatac cctgatggga 660 gacgatccgg agccgcccgt gcagcctttc gatccaccta cgcttcacga actgtatgat 720 ttagaggtag acgggccgga ggatcccaat gaggaagctg tgaatgggtt ttttactgat 780 tctatgctgc tagctgctga ggaaggattg gacgtaaacc ctcctccgga gacccttgat 840 accccagggg tggttgtgga aagcggcaga ggtgggaaaa aattgcctga tctgggagca 900 gctgaaatgg acttgcgttg ttatgaagag ggttttcctc cgagtgatga tgaagatgag 960 gaaagtgagc agtccatcca gaccgcagtg aatgagggag tgaaagctgc cagtgatgtt 1020 tttaagttgg actgtccgga gctgcctgga catggctgta agtcttgtga atttcacagg 1080 aataacactg gaatgaaaga actattatgc tcgctttgct atatgagaac gcactgccac 1140 tttatttaca gtaagtgtgt ttaagtgaaa tttaaaggaa cagtgaagct gttttaataa 1200 ctttgttgaa tgggggattt atgttttact tgtgattttt ttataggtcc tgtgtctgat 1260 gatgattcgc cttctcctga ttcaactacc tcacctcctg aaattcaggc gcccgtccct 1320 gcaaacgtat gcaagcccat tcctgtgaag cctaagcctg ggaaacgccc tgctgtggat 1380 aagcttgagg acttgttgga gggtggggat ggacctttgg actttagtac ccggaaactg 1440 ccaaggcaat gagtgccctg cacctgtgtt tatttaatgt gacgtcagta tttatgtgag 1500 agtgccatgt aataaaatta tgtcagctgc tgagtgtttt attgcttctt gggtggggac 1560 ttggatatat aagtaggagc agacctgtgt ggttagctca cagcagcctg ctgccatcca 1620 tggaggtttg ggctatcttg gaagacctta gacagactag gctactgcta gaaaacgcct 1680 cggacggagt ctctggcctt tggagattct ggttcggtgg tgatctagct aggctagtct 1740 ttaggataaa acaggactac agggaagaat ttgaaaagtt attggacgac agtccaggac 1800 tttttgaagc tcttaacttg ggccatcagg ctcattttaa ggagaaggtt ttatcagttt 1860 tagatttttc tactcctggt agaactgctg ctgctgtagc ctttcttact tttatattgg 1920 ataa atg gat ccg cca aac cca ctt cag caa ggg ata cgt ttt gga ttt 1969 Met Asp Pro Pro Asn Pro Leu Gln Gln Gly Ile Arg Phe Gly Phe 1 5 10 15 cat agc agc agc ttt gtg gag aac atg gaa ggc tcg cag gat gag gac 2017 His Ser Ser Ser Phe Val Glu Asn Met Glu Gly Ser Gln Asp Glu Asp 20 25 30 aat ctt aga tta ctg gcc agt gca gcc tct ggg cgt agc agg gat cct 2065 Asn Leu Arg Leu Leu Ala Ser Ala Ala Ser Gly Arg Ser Arg Asp Pro 35 40 45 gag aca ccc acc ggc cat gcc agc ggt tct gga gga gga gca gca gga 2113 Glu Thr Pro Thr Gly His Ala Ser Gly Ser Gly Gly Gly Ala Ala Gly 50 55 60 gga caa tcc gag agc cgg cct gga ccc tcc ggt gga gga ggc gga gga 2161 Gly Gln Ser Glu Ser Arg Pro Gly Pro Ser Gly Gly Gly Gly Gly Gly 65 70 75 gta gct gac ctg ttt cct gaa ctg cga cgg gtg ctt act agg tct acg 2209 Val Ala Asp Leu Phe Pro Glu Leu Arg Arg Val Leu Thr Arg Ser Thr 80 85 90 95 tcc agt gga cag gac agg ggc att aag agg gag agg aat cct agt ggg 2257 Ser Ser Gly Gln Asp Arg Gly Ile Lys Arg Glu Arg Asn Pro Ser Gly 100 105 110 cat aat tca aga act gag ttg gct tta agt tta atg agt cgc agg cgt 2305 His Asn Ser Arg Thr Glu Leu Ala Leu Ser Leu Met Ser Arg Arg Arg 115 120 125 cct gaa act gtt tgg tgg cat gag gtt cag agc gaa ggc agg gat gaa 2353 Pro Glu Thr Val Trp Trp His Glu Val Gln Ser Glu Gly Arg Asp Glu 130 135 140 gtt tca ata ttg cag gag aaa tat tcc cta gaa caa ctt aag acc tgt 2401 Val Ser Ile Leu Gln Glu Lys Tyr Ser Leu Glu Gln Leu Lys Thr Cys 145 150 155 tgg ttg gaa cct gag gat gat tgg gag gtg gcc att ggg aat tat gct 2449 Trp Leu Glu Pro Glu Asp Asp Trp Glu Val Ala Ile Gly Asn Tyr Ala 160 165 170 175 aag ata tct ctg agg cct gat aaa cag tat aga att act aag aag att 2497 Lys Ile Ser Leu Arg Pro Asp Lys Gln Tyr Arg Ile Thr Lys Lys Ile 180 185 190 aat atc aga aat gca tgc tac ata tca ggg aat ggg gcc gag gtt ata 2545 Asn Ile Arg Asn Ala Cys Tyr Ile Ser Gly Asn Gly Ala Glu Val Ile 195 200 205 ata gat acg caa gat aaa gca gct ttt aga tgt tgt atg atg ggt atg 2593 Ile Asp Thr Gln Asp Lys Ala Ala Phe Arg Cys Cys Met Met Gly Met 210 215 220 tgg cca ggg gtg gtc ggc atg gaa gca gta aca ctt atg aat att agg 2641 Trp Pro Gly Val Val Gly Met Glu Ala Val Thr Leu Met Asn Ile Arg 225 230 235 ttt aga ggg gat ggg tat aat ggg att gtc ttt atg gct aac act aag 2689 Phe Arg Gly Asp Gly Tyr Asn Gly Ile Val Phe Met Ala Asn Thr Lys 240 245 250 255 ctg att ctg cat ggt tgt agc ttt ttt ggg ttt aat aat act tgt gta 2737 Leu Ile Leu His Gly Cys Ser Phe Phe Gly Phe Asn Asn Thr Cys Val 260 265 270 gaa gct tgg ggg caa gtc agt gta agg ggt tgt agt ttt tat gca tgc 2785 Glu Ala Trp Gly Gln Val Ser Val Arg Gly Cys Ser Phe Tyr Ala Cys 275 280 285 tgg att gca aca tca ggt agg gtc aag agt cag ttg tct gtg aag aaa 2833 Trp Ile Ala Thr Ser Gly Arg Val Lys Ser Gln Leu Ser Val Lys Lys 290 295 300 tgc atg ttt gag aga tgt aat ctg ggc ata ctg aat gaa ggc gaa gca 2881 Cys Met Phe Glu Arg Cys Asn Leu Gly Ile Leu Asn Glu Gly Glu Ala 305 310 315 agg gtc cgc cac tgc gct gct aca gaa act ggc tgc ttc att cta ata 2929 Arg Val Arg His Cys Ala Ala Thr Glu Thr Gly Cys Phe Ile Leu Ile 320 325 330 335 aag gga aat gcc agt gtg aag cat aac atg atc tgt gga cct tcg gat 2977 Lys Gly Asn Ala Ser Val Lys His Asn Met Ile Cys Gly Pro Ser Asp 340 345 350 gag agg cct tat cag atg ctg acc tgc gct ggt gga cat tgc aat atg 3025 Glu Arg Pro Tyr Gln Met Leu Thr Cys Ala Gly Gly His Cys Asn Met 355 360 365 ctt gct acc gtg cat atc gtt tct cat gca cgc aag aaa tgg cct gta 3073 Leu Ala Thr Val His Ile Val Ser His Ala Arg Lys Lys Trp Pro Val 370 375 380 ttc gaa cat aat gtg atg acc aag tgc acc atg cac ata ggt ggt cgc 3121 Phe Glu His Asn Val Met Thr Lys Cys Thr Met His Ile Gly Gly Arg 385 390 395 agg gga atg ttt atg cct tac cag tgt aac atg aat cat gtg aag gtg 3169 Arg Gly Met Phe Met Pro Tyr Gln Cys Asn Met Asn His Val Lys Val 400 405 410 415 atg ttg gaa cca gat gcc ttt tcc aga atg agc tta aca gga atc ttt 3217 Met Leu Glu Pro Asp Ala Phe Ser Arg Met Ser Leu Thr Gly Ile Phe 420 425 430 gat atg aat gtg caa cta tgg aag atc ctg aga tat gat gac acc aaa 3265 Asp Met Asn Val Gln Leu Trp Lys Ile Leu Arg Tyr Asp Asp Thr Lys 435 440 445 tcg agg gtg cgc gca tgc gaa tgc gga ggc aag cat gcc aga ttc cag 3313 Ser Arg Val Arg Ala Cys Glu Cys Gly Gly Lys His Ala Arg Phe Gln 450 455 460 ccg gtg tgt gtg gat gtg act gaa gac ctg aga ccc gat cat ttg gtg 3361 Pro Val Cys Val Asp Val Thr Glu Asp Leu Arg Pro Asp His Leu Val 465 470 475 ctt gcc tgc act gga gcg gag ttc ggt tct agt ggg gaa gaa act gac 3409 Leu Ala Cys Thr Gly Ala Glu Phe Gly Ser Ser Gly Glu Glu Thr Asp 480 485 490 495 taaagtgagt agtggggcat gctgtggagg gtattccagg cgggtaaggt gggcagattg 3469 ggtaaattct gtttgtttct gtcttgcagc tacc atg agt gga agc gct tct ttt 3524 Met Ser Gly Ser Ala Ser Phe 500 gag ggg gga gtc ttt agc cct tat ctg acg ggc agg ctc cca ccc tgg 3572 Glu Gly Gly Val Phe Ser Pro Tyr Leu Thr Gly Arg Leu Pro Pro Trp 505 510 515 gca gga gtt cgt cag aat gtc atg gga tcc act gtg gat ggg aga ccc 3620 Ala Gly Val Arg Gln Asn Val Met Gly Ser Thr Val Asp Gly Arg Pro 520 525 530 gtc cag ccc gcc aat tcc tca acg ctg acc tat gcc act ttg agc tct 3668 Val Gln Pro Ala Asn Ser Ser Thr Leu Thr Tyr Ala Thr Leu Ser Ser 535 540 545 550 tca ccc ttg gat gca gct gca gcc gcc gcc gcc tct gct gcc gcc aac 3716 Ser Pro Leu Asp Ala Ala Ala Ala Ala Ala Ala Ser Ala Ala Ala Asn 555 560 565 acc gtc ctt gga atg ggc tat tat gga agc atc gtt gcc aat tcc agt 3764 Thr Val Leu Gly Met Gly Tyr Tyr Gly Ser Ile Val Ala Asn Ser Ser 570 575 580 tcc tct aat aac cct tcg acc ctg gct gag gac aag cta ctt gtc ctc 3812 Ser Ser Asn Asn Pro Ser Thr Leu Ala Glu Asp Lys Leu Leu Val Leu 585 590 595 ttg gct cag ctt gag gcc ttg acc cag cgc cta ggc gaa ctg tct cag 3860 Leu Ala Gln Leu Glu Ala Leu Thr Gln Arg Leu Gly Glu Leu Ser Gln 600 605 610 cag gtg gcc cag ttg cgc gag caa act gag tct gct gtt gcc aca gca 3908 Gln Val Ala Gln Leu Arg Glu Gln Thr Glu Ser Ala Val Ala Thr Ala 615 620 625 630 aag tct aaa taaagattcc caaatcaata aataaaggag atccttgttg 3957 Lys Ser Lys attgtaaaac aagtgtaatg aatctttatt tgatttttcg cgcgcggtat gccctggacc 4017 accggtctcg atcattgaga actcggtgga tcttttccag gatcctgtag aggtgggatt 4077 gaatgtttag atacatgggc attaggccgt ctcgggggtg gagatagctc cattgaagag 4137 cctcatgctc cggggtagtg ttataaatca cccagtcata acaaggtcgg agtgcatggt 4197 gttgcacaat atcttttagg agcaggctaa ttgcaacggg gaggccctta gtgtaggtgt 4257 ttacaaatct gttgagctgg gacgggtgca tccggggtga aattatatgc attttggact 4317 gaatcttaag gttggcaatg ttgccgccta gatcccgtct cgggttcata ttgtgcagga 4377 ccaccaagac agtgtatccg gtgcacttgg gaaatttatc atgcagctta gagggaaaag 4437 catgaaaaaa tttggagacg cctttgtggc cgcccagatt ctccatgcac tcatccataa 4497 tgatagcgat ggggccgtgg gcggcggcgc gggcgaacac gttccggggg tctgacacat 4557 catagttatg ctcctgagtc aggtcatcat aagccatttt aataaacttg gggcggaggg 4617 tgccagattg ggggatgaaa gttccctcgg gccccggagc atagtttccc tcacatattt 4677 gcatttccca ggctttcagt tcagaggggg ggatcatgtc cacctgcggg gctataaaaa 4737 ataccgtttc tggagccggg gtgattaact gggatgagag caaattcctg agcagctgag 4797 acttgccgca cccagtggga ccgtaaatga ccccgattac gggttgcaga tggtagttta 4857 gggagcggca gctgccgtcc tcccggagca ggggggccac ttcgttcatc atttccctta 4917 catggatatt ttcccgcacc aagtccgtta ggaggcgctc tcccccaagg gatagaagct 4977 cctggagcga ggagaagttt ttcagcggct tcagcccgtc agccatgggc attttggaga 5037 gagtctgttg caagagctcg agccggtccc agagctcggt gatgtgttct atggcatctc 5097 tatccagcag acctcctcgt ttcgcgggtt gggacggctc ctggagtagg gaatcagacg 5157 atgggcgtcc agcgctgcca gggtccgatc cttccatggt cgcagcgtcc gagtcagggt 5217 tgtttccgtc acggtgaagg ggtgcgcgcc tggttgggcg cttgcgaggg tgcgcttcag 5277 gctcatcctg ctggtcgaga accgctgccg atcggcgccc tgcatgtcgg ccaggtagca 5337 gtttaccatg agttcgtagt tgagagcctc ggccgcgtgg cctttggcgc ggagcttacc 5397 tttggaagtt ttctggcagg cggggcagta gagacacttg agggcataca gcttgggcgc 5457 gaggaagatg gattcggggg agtatgcatc cgcaccgcag gaggcgcaga cggtttcgca 5517 ctccacgagc caggtcagat ccggctcatc ggggtcaaaa acaagttttc cgccatgttt 5577 tttgatgcgt ttcttacctt tggtttccat gagttcgtgt ccccgctggg tgacaaagag 5637 gctgtccgtg tccccgtaga ccgactttat gggcctgtcc tcgagcggag tgccttggtc 5697 ctcttcgtag aggaacccag tccactctga tacaaaggcg cgcgtccagg ccagcacaaa 5757 ggaggccacg tgggaggggt agcggtcgtt gtcaaccagg gggtccacct tctctacggt 5817 atgtaaacac atgtccccct cctccacatc caagaatgtg attggcttgt aagtgtaggc 5877 cacgtgacca gtggtccccg ccgggggggt ataaaagggg gcgggcctct gttcgtcctc 5937 actgtcttcc ggatcgctgt ccaggagcgc cagctgttgg ggtaggtatt ccctctcgaa 5997 ggcgggcatg acctctgcac tcaggttgtc agtttctagg aacgaggagg atttgatatt 6057 gacagtacca gccgagatgc ctttcatgag actttcgtcc atctggtcag aaaatacaat 6117 cttcttgttg tccagcttgg tggcaaatga tccatagagg gcattggata gaagcttggc 6177 gatggagcgc atggtttggt tcttttcctt gtccgcgcgc tccttggcgg cgatgttgag 6237 ctggacgtac tcgcgcgcca cacatttcca ttcagggaag atggttgtca gttcatctgg 6297 aactattctg actcgccatc ccctattgtg cagggttatc agatccacac tggtggccac 6357 ctcgcctcgg aggggctcat tggtccagca gagtcgacct ccttttcttg aacagaaagg 6417 ggggaggggg tctagcatga gctcatcagg ggggtccgca tctatggtga atattcccgg 6477 gagcagatcc ttgtcaaaat agctgatggt ggcgggatca tccaaagtca tctgccattc 6537 tcgagctgcc agcgcgcgct cataggggtt gagaggggtg ccccagggca tggggtgggt 6597 gagcgcggag gcatacatgc cacagatatc atagacatag aggggctctt cgaggatgcc 6657 gatgtaagtg ggataacagc gcccccctct gatgcttgct cgcacatagt catagagttc 6717 atgtgagggg gcgagaagac ccgggcccag attggtgcgg ttgggttttt ccgccctgta 6777 aacgatctgg cgaaagatgg catgggaatt ggaagagatg gtaggtctct gaaagatgtt 6837 aaaatgggca tgaggtaggc ctacagagtc ccttatgaag tgggcatatg actcttgcag 6897 cttggctacc agctcggcgg tgacgagtac atccagggca cagtagtcga gagtctcttg 6957 gatgatgtca taacgcggtt ggcttttctt ttcccacagc tcgcggttga gaaggtattc 7017 ttcgcgatcc ttccagtact cttcgagggg aaacccgtct ttgtctgcac ggtaagagcc 7077 cagcatgtag aactgattga ctgccttgta gggacagcat cccttctcca ctgggagaga 7137 gtatgcttgg gctgccttgc gcagcgaggt atgagtgagg gcaaaagtgt ccctgaccat 7197 gactttgagg aattgatact tgaagtcgat gtcatcacag gccccctgtt cccagagttg 7257 gaagtccacc cgcttcttgt aggcggggtt gggcaaagcg aaagtaacat cattgaagag 7317 gatcttgccg gccctgggca tgaaatttcg ggtgattctg aaaggctgag gcacctctgc 7377 tcggttattg ataacctgag cggccaagac gatctcatca aagccattga tgttgtgccc 7437 cactatgtac agttctatga atcgaggggt gcccctgaca tgaggcagct tcttgagttc 7497 ttcaaaagtg aggtctgtag ggtcagtgag agcatagtgt tcgagggccc attcgtgcag 7557 gtgagggttc gctttgagga aggaggacca gaggtccact gccagtgctg tttgtaactg 7617 gtcccggtac tggcgaaaat gctggccgac tgccatcttt tctggggtga cgcagtagaa 7677 ggttttgggg tcctgctgcc agcgatccca cttgagtttc atggcgaggt cataggcgat 7737 gttgacgagc cgctcgtccc cagagagttt catgaccagc atgaagggga ttagctgctt 7797 gccaaaggac cccatccagg tgtaggtttc cacatcgtag gtgaggaaga gcctttctgt 7857 gcgaggatga gagccgatcg ggaagaactg gatctcctgc caccagttgg aggaatggct 7917 gttgatgtga tggaagtaga actccctgcg gcgcgccgag cattcatgct tgtgcttgta 7977 cagacggccg cagtactcgc agcgcttcac gggatgcacc tcatgaatga gttgtacctg 8037 gcttcctttg acgagaaatt tcagtgggaa gttgaggcct ggcgcttgta cctcgcgctc 8097 tactatgttg tctgcatcgg cctggccatc ttctgtctcg atggtggtca tgctgacgag 8157 cccccgcggg aggcaagtcc agacctcggc gcggcagggg cggagctcga ggacgagagc 8217 gcgcaggccg gagttgtcca gggtcctgag acgctgcgga gtcaggttag taggtagtgt 8277 caggagattg acttgcatga tcttttcgag ggcgtgcggg aggttcagat ggtacttgat 8337 ctccacgggt ccgttggtgg agatgtcgat ggcttgcagg gttccgtgcc ccttgggcgc 8397 taccaccgtg cccttgtttt tccttttggg cggcggtggc tctgttgctt cttgcatgtt 8457 cagaagcggt ggcgagggcg cgcgccgggc ggcaggggcg gctcgggacc cggcggcatg 8517 gctggcagtg gcacgtcggc gccgcgcgcg ggtaggttct ggtactgcgc cctgagaaga 8577 cttgcgtgcg cgacgacgcg gcggttgacg tcctggatct gacgcctctg ggtgaaagct 8637 accggccccg tgagcttgaa cctgaaagag agttcaacag aatcaatctc ggtatcgttg 8697 acggcggctt gcctcaggat ctcttgcacg tcgcccgagt tgtcctggta ggcgatctcg 8757 gccatgaact gctcgatctc ttcctcttga agatctccgc ggcccgctct ctcgacggtg 8817 gccgcaaggt cgttggagat gcgccccatg agttgagaga atgcattcat gcccgcctcg 8877 ttccagacgc ggctgtagac cacggccccc tcgggatctc tcgcgcgcat gaccacctgg 8937 gcgaggttga gctccacgtg gcgggtgaag accgcatagt tgcataggcg ctggaaaagg 8997 tagttgagtg tggtggcgat gtgctcggca acgaagaaat acatgatcca tcgtctcagc 9057 ggcatctcgc tgacatcgcc cagggcttcc aagcgctcca tggcctcgta gaagtccacg 9117 gcgaagttga aaaactggga gttgcgcgcg gacacggtca actcctcttc cagaagacgg 9177 atgagttcgg cgatggtggc gcgcacctca cgctcgaaag cccccgggat ttcttcctcc 9237 tcctcttcct caatctcttc ttcttccact aacatctctt cttcctcttc aggtgggggt 9297 ggaggaggag ggggaacgcg gcgacgccgg cggcgcacgg gcagacggtc gataaatctt 9357 tcaatgacct ctccgcggcg gcggcgcatg gtctcggtga cggcacgacc gttctccctg 9417 ggtctcagag tgaagacgcc tccgcgcatc tccctgaagt ggtgactggg aggctctccg 9477 ttgggcaggg acagggcgct gattatgcat tttatcaatt gccccgtagg gactccgcgc 9537 aaggacctga tcgtctcaag atccacggga tctgaaaacc tttcgacgaa agcgtctaac 9597 cagtcgcaat cgcaaggtag gctgagcact gtttcttgcg ggcgggggcg gctagacgct 9657 tggtcggggt tctctctttc ttctccttcc tcctcttggg agggtgagac gatgctgctg 9717 gtgatgaaat taaaataggc agttttgaga cggcggatgg tggcgaggag caccaggtct 9777 ttgggaccgg cttgttggat gcgcaggcga tgggccattc cccaagcatt atcctggcat 9837 ctggccagat ctttatagta gtcttgcatg agtcgttcca cgggcacttc ttcttcgccc 9897 gctctgccat gcatgcgagt gagcccgaac ccgcgcatgg gctggacaag tgccaggtcc 9957 gctactaccc tttcggcgag gatggcttgc tgcacctggg tgagggtggc ttggaagtcg 10017 tcaaagtcca cgaagcggtg gtaggccccg gtgttaatgg tgtaggagca gttggccatg 10077 actgaccagt tgactgtctg gtgccccggg cgcacgagct cggtgtactt gaggcgcgag 10137 taggcgcggg tgtcaaagat gtaatcgttg caggtgcgca ccaggtactg gtagccgatg 10197 agaaagtgtg gcggtggctg gcggtagagg ggccatcgct ctgtagccgg ggcgccaggg 10257 gcgaggtctt ccagcatgag gcggtggtag ccgtagatgt acctggacat ccaggtgata 10317 ccggaggcgg tggttgatgc acgtgggaac tcgcgcacgc ggttccagat gttgcgcagc 10377 ggcatgaagt agttcatggt aggcacggtc tggccagtga ggcgcgcgca gtcattgatg 10437 ctctatagac acggagaaaa cgaaagcgat gagcggctcg actccgtggc ctggaggaac 10497 gtgaacgggt tgggtcgcgg tgtaccccgg ttcgagacca aagccaagcg agcacactcg 10557 gatcggccgg agccgcggct aacgtggtat tggcaatccc gtctcgaccc agccgacgaa 10617 tatccaggat acggagtaga gtcgtttttg ctgcttgttg ctttttcctg gacgggtgcc 10677 agtgccgcgt caagctttag aacgctcagt tctcggggcc gggagtggct cgcgcccgta 10737 gtctggagaa tcaatcgcca gggttgcgtt gcggtatgcc ccggttcgag cctcagcgcg 10797 gctcgtatcg gccggtttcc gcggcaagcg agggtttggc agccccgtca tttctaagac 10857 cccgccagcc gacttctcca gtttacggga gcgagccctc tttttttttt gttttttgtc 10917 gcccag atg cat cca gtg ctg cga cag atg cgc ccc cag caa cag gcc 10965 Met His Pro Val Leu Arg Gln Met Arg Pro Gln Gln Gln Ala 635 640 645 cct tct cag caa cag cag cta cag caa cag cca caa aag gct ctt cct 11013 Pro Ser Gln Gln Gln Gln Leu Gln Gln Gln Pro Gln Lys Ala Leu Pro 650 655 660 gct cct gca act act gca gct gca gcc gtg agc ggc gcg gga cag ccc 11061 Ala Pro Ala Thr Thr Ala Ala Ala Ala Val Ser Gly Ala Gly Gln Pro 665 670 675 gcc tat gat ctg gac ttg gaa gag ggc gag gga ttg gcg cgc ctg ggg 11109 Ala Tyr Asp Leu Asp Leu Glu Glu Gly Glu Gly Leu Ala Arg Leu Gly 680 685 690 695 gct ccc tcg ccc gag cgg cac ccg cgg gtg caa ctg aaa aag gac tct 11157 Ala Pro Ser Pro Glu Arg His Pro Arg Val Gln Leu Lys Lys Asp Ser 700 705 710 cgc gag gcg tac gtg ccc cat cag aac ctg ttc agg gac agg agc ggc 11205 Arg Glu Ala Tyr Val Pro His Gln Asn Leu Phe Arg Asp Arg Ser Gly 715 720 725 gag gag ccc gag gag atg cga gca tct cga ttt aac gcg ggt cgc gag 11253 Glu Glu Pro Glu Glu Met Arg Ala Ser Arg Phe Asn Ala Gly Arg Glu 730 735 740 ctg cgc cac ggt ctg gat cga aga cgg gtg ctg cgg gac gag gat ttc 11301 Leu Arg His Gly Leu Asp Arg Arg Arg Val Leu Arg Asp Glu Asp Phe 745 750 755 gag gtt gat gag gcg acg ggg atc agc tcc gct agg gca cat gtg gcc 11349 Glu Val Asp Glu Ala Thr Gly Ile Ser Ser Ala Arg Ala His Val Ala 760 765 770 775 gcg gcc aac ctt gtc tcg gcc tac gag cag acc gtg aag gag gag cgc 11397 Ala Ala Asn Leu Val Ser Ala Tyr Glu Gln Thr Val Lys Glu Glu Arg 780 785 790 aac ttc caa aaa tct ttc aac aac cat gtg cgc act ctg atc gcc cgc 11445 Asn Phe Gln Lys Ser Phe Asn Asn His Val Arg Thr Leu Ile Ala Arg 795 800 805 gag gaa gtg acc ctg ggt ctg atg cac ctg tgg gac ctg atg gaa gcc 11493 Glu Glu Val Thr Leu Gly Leu Met His Leu Trp Asp Leu Met Glu Ala 810 815 820 atc acc cag aac ccc act agc aaa ccc ctg aca gcc cag ctg ttt ctg 11541 Ile Thr Gln Asn Pro Thr Ser Lys Pro Leu Thr Ala Gln Leu Phe Leu 825 830 835 gta gtg caa cat agc agg gac aat gag gcg ttc agg gag gcg ctg ctg 11589 Val Val Gln His Ser Arg Asp Asn Glu Ala Phe Arg Glu Ala Leu Leu 840 845 850 855 aac atc acc gag ccc gag ggg aga tgg ttg tat gat ctg atc aat atc 11637 Asn Ile Thr Glu Pro Glu Gly Arg Trp Leu Tyr Asp Leu Ile Asn Ile 860 865 870 ctg caa agc att gta gtg cag gaa cgc agc ctg ggt ctg gcc gag aaa 11685 Leu Gln Ser Ile Val Val Gln Glu Arg Ser Leu Gly Leu Ala Glu Lys 875 880 885 gtg gct gcc att aac tac tcg gtc ttg agt ctg ggc aag tac tac gct 11733 Val Ala Ala Ile Asn Tyr Ser Val Leu Ser Leu Gly Lys Tyr Tyr Ala 890 895 900 cgc aag atc tac aag acc ccc tac gtg ccc ata gac aag gag gtg aag 11781 Arg Lys Ile Tyr Lys Thr Pro Tyr Val Pro Ile Asp Lys Glu Val Lys 905 910 915 ata gat ggg ttt tac atg cgc atg act ctc aag gtg ctg act ctc agt 11829 Ile Asp Gly Phe Tyr Met Arg Met Thr Leu Lys Val Leu Thr Leu Ser 920 925 930 935 gac gat ctg ggg gtg tac cgc aac gac agg atg cac cgc gcg gtg agc 11877 Asp Asp Leu Gly Val Tyr Arg Asn Asp Arg Met His Arg Ala Val Ser 940 945 950 gcc agc agg agg cgc gag ctg agc gac aga gaa ctt atg cac agc ttg 11925 Ala Ser Arg Arg Arg Glu Leu Ser Asp Arg Glu Leu Met His Ser Leu 955 960 965 caa aga gct ctg acg ggg gct ggg acc gag ggg gag aac tac ttt gac 11973 Gln Arg Ala Leu Thr Gly Ala Gly Thr Glu Gly Glu Asn Tyr Phe Asp 970 975 980 atg gga gcg gac ttg caa tgg cag ccc agc cgc agg gcc ctg gag gct 12021 Met Gly Ala Asp Leu Gln Trp Gln Pro Ser Arg Arg Ala Leu Glu Ala 985 990 995 gcg ggg tgt gag ctt cct tac ata gaa gag gtg gat gaa ggc gag 12066 Ala Gly Cys Glu Leu Pro Tyr Ile Glu Glu Val Asp Glu Gly Glu 1000 1005 1010 gac gag gag ggc gag tac ctg gaa gac tgatggcgcg acccgtattt 12113 Asp Glu Glu Gly Glu Tyr Leu Glu Asp 1015 1020 ttgctag atg gaa cag cag cag gca ccg gac ccc gca atg cgg gcg 12159 Met Glu Gln Gln Gln Ala Pro Asp Pro Ala Met Arg Ala 1025 1030 1035 gcg ctg cag agc cag ccg tcc ggc att aac tcc tcg gac gat tgg 12204 Ala Leu Gln Ser Gln Pro Ser Gly Ile Asn Ser Ser Asp Asp Trp 1040 1045 1050 acc cag gcc atg caa cgc atc atg gcg ctg acg acc cgc aac ccc 12249 Thr Gln Ala Met Gln Arg Ile Met Ala Leu Thr Thr Arg Asn Pro 1055 1060 1065 gaa gcc ttt aga cag caa ccc cag gcc aac cgc ctt tcg gcc atc 12294 Glu Ala Phe Arg Gln Gln Pro Gln Ala Asn Arg Leu Ser Ala Ile 1070 1075 1080 ctg gag gcc gta gtt cct tcc cgc tcc aac ccc acc cac gag aag 12339 Leu Glu Ala Val Val Pro Ser Arg Ser Asn Pro Thr His Glu Lys 1085 1090 1095 gtc ctg gcc atc gtg aac gcg ctg gtg gag aac aag gcc atc cgt 12384 Val Leu Ala Ile Val Asn Ala Leu Val Glu Asn Lys Ala Ile Arg 1100 1105 1110 ccc gat gag gcc ggg ctg gta tac aat gcc ctc ttg gag cgc gtg 12429 Pro Asp Glu Ala Gly Leu Val Tyr Asn Ala Leu Leu Glu Arg Val 1115 1120 1125 gcc cgc tac aac agc agc aac gtg cag acc aac ctg gat cgg atg 12474 Ala Arg Tyr Asn Ser Ser Asn Val Gln Thr Asn Leu Asp Arg Met 1130 1135 1140 gtg tcc gat gtg cgc gag gcc gtg tct cag cgc gag cgg ttc cag 12519 Val Ser Asp Val Arg Glu Ala Val Ser Gln Arg Glu Arg Phe Gln 1145 1150 1155 cgc gac gcc aac ttg ggg tcg ctg gta gcg ctg aac gcc ttc ctc 12564 Arg Asp Ala Asn Leu Gly Ser Leu Val Ala Leu Asn Ala Phe Leu 1160 1165 1170 agc acc cag ccc gcc aac gtg ccc cgt ggc cag caa gac tat aca 12609 Ser Thr Gln Pro Ala Asn Val Pro Arg Gly Gln Gln Asp Tyr Thr 1175 1180 1185 aac ttt ttg agt gca ttg aga ctc atg gta gct gag gtg ccc cag 12654 Asn Phe Leu Ser Ala Leu Arg Leu Met Val Ala Glu Val Pro Gln 1190 1195 1200 agc gag gtg tac cag tcc ggg cca gat tac ttc ttc cag acc agt 12699 Ser Glu Val Tyr Gln Ser Gly Pro Asp Tyr Phe Phe Gln Thr Ser 1205 1210 1215 aga cag ggc ttg cag aca gtg aac ctg acc cag gct ttc aag aac 12744 Arg Gln Gly Leu Gln Thr Val Asn Leu Thr Gln Ala Phe Lys Asn 1220 1225 1230 ctg aag ggt ctg tgg gga gtg cac gcc ccg gta ggg gat cgc gcg 12789 Leu Lys Gly Leu Trp Gly Val His Ala Pro Val Gly Asp Arg Ala 1235 1240 1245 acc gtg tct agc ttg ctg act ccc aac tcc cgc ctg ctg ctg ctg 12834 Thr Val Ser Ser Leu Leu Thr Pro Asn Ser Arg Leu Leu Leu Leu 1250 1255 1260 ctg gta tcc ccc ttc act gac agc ggt agc att gac cgc aac tcc 12879 Leu Val Ser Pro Phe Thr Asp Ser Gly Ser Ile Asp Arg Asn Ser 1265 1270 1275 tac ttg ggc tac ctg ctg aac ctg tat cgc gag gcc ata ggg cag 12924 Tyr Leu Gly Tyr Leu Leu Asn Leu Tyr Arg Glu Ala Ile Gly Gln 1280 1285 1290 agc cag gtg gac gag cag acc tac cag gaa atc acc caa gtg agc 12969 Ser Gln Val Asp Glu Gln Thr Tyr Gln Glu Ile Thr Gln Val Ser 1295 1300 1305 cgc gcc ctg ggt cag gaa gac acg ggc agt ttg gaa gcc acc ctg 13014 Arg Ala Leu Gly Gln Glu Asp Thr Gly Ser Leu Glu Ala Thr Leu 1310 1315 1320 aac ttc ttg ctg acc aac cgg tcg cag aag atc cct cct cag tac 13059 Asn Phe Leu Leu Thr Asn Arg Ser Gln Lys Ile Pro Pro Gln Tyr 1325 1330 1335 gcg ctt acc gcg gag gag gag cgg atc ctg aga tac gtg cag cag 13104 Ala Leu Thr Ala Glu Glu Glu Arg Ile Leu Arg Tyr Val Gln Gln 1340 1345 1350 agc gtt gga ctg ttc ctg atg cag gag ggg gcg acc cct acc gcc 13149 Ser Val Gly Leu Phe Leu Met Gln Glu Gly Ala Thr Pro Thr Ala 1355 1360 1365 gcg ctg gac atg aca gct cga aac atg gag ccc agc atg tat gcc 13194 Ala Leu Asp Met Thr Ala Arg Asn Met Glu Pro Ser Met Tyr Ala 1370 1375 1380 agt aac cga cct ttc atc aac aaa ctg ctg gac tac ctg cac agg 13239 Ser Asn Arg Pro Phe Ile Asn Lys Leu Leu Asp Tyr Leu His Arg 1385 1390 1395 gca gcc gcc atg aac tct gat tat ttc acc aat gct atc ttg aac 13284 Ala Ala Ala Met Asn Ser Asp Tyr Phe Thr Asn Ala Ile Leu Asn 1400 1405 1410 ccc cac tgg ttg ccc ccg cct ggt ttc tac acg ggc gag tac gac 13329 Pro His Trp Leu Pro Pro Pro Gly Phe Tyr Thr Gly Glu Tyr Asp 1415 1420 1425 atg ccc gac cca aat gac ggg ttc ctg tgg gac gat gtg gac agc 13374 Met Pro Asp Pro Asn Asp Gly Phe Leu Trp Asp Asp Val Asp Ser 1430 1435 1440 agc ata ttc tcc ccg cct ccc ggt tat acc gtt tgg aag aag gaa 13419 Ser Ile Phe Ser Pro Pro Pro Gly Tyr Thr Val Trp Lys Lys Glu 1445 1450 1455 ggg ggc gat aga agg cac tct tcc gtg tcg ctg tcc gga acg gct 13464 Gly Gly Asp Arg Arg His Ser Ser Val Ser Leu Ser Gly Thr Ala 1460 1465 1470 ggt gct gcc gct gcg gtg ccc gaa gct gca agt cct ttc cct agc 13509 Gly Ala Ala Ala Ala Val Pro Glu Ala Ala Ser Pro Phe Pro Ser 1475 1480 1485 ttg ccc ttt tca cta aac agc gtt cgc agc agt gaa ctg ggg aga 13554 Leu Pro Phe Ser Leu Asn Ser Val Arg Ser Ser Glu Leu Gly Arg 1490 1495 1500 ata acc cgc ccg cgc tta atg ggc gag gat gag tac ttg aat gac 13599 Ile Thr Arg Pro Arg Leu Met Gly Glu Asp Glu Tyr Leu Asn Asp 1505 1510 1515 tct ttg ctg agg cca gag agg gaa aag aac ttc ccc aac aat gga 13644 Ser Leu Leu Arg Pro Glu Arg Glu Lys Asn Phe Pro Asn Asn Gly 1520 1525 1530 ata gaa agc ctg gtg gat aag atg agt aga tgg aag acc tat gcg 13689 Ile Glu Ser Leu Val Asp Lys Met Ser Arg Trp Lys Thr Tyr Ala 1535 1540 1545 cag gat cac aga gat gag cct agg atc ttg ggg gct aca agc gga 13734 Gln Asp His Arg Asp Glu Pro Arg Ile Leu Gly Ala Thr Ser Gly 1550 1555 1560 gcg acc cgt aga cgc cag cgg cat gac agg cag agg ggt ctt gtg 13779 Ala Thr Arg Arg Arg Gln Arg His Asp Arg Gln Arg Gly Leu Val 1565 1570 1575 tgg gac gat gag gac tcg gcc gat gac agc agc gtg ttg gac ttg 13824 Trp Asp Asp Glu Asp Ser Ala Asp Asp Ser Ser Val Leu Asp Leu 1580 1585 1590 ggt ggg aga gga gtg ggc aac ccg ttc gct cat ctg cgt ccc cga 13869 Gly Gly Arg Gly Val Gly Asn Pro Phe Ala His Leu Arg Pro Arg 1595 1600 1605 ttt gga cgc atg ttg taaaagtgaa agtaaaataa aaaggcaact caccaaggcc 13924 Phe Gly Arg Met Leu 1610 atggcgaccg agcgtgcgtt cgttcttttc tgttatctgt gtctagt atg atg agg 13980 Met Met Arg agg cga gcc gtg cta ggt gga gcg gtg gtg tat ccg gag ggt cct 14025 Arg Arg Ala Val Leu Gly Gly Ala Val Val Tyr Pro Glu Gly Pro 1615 1620 1625 cct cct tcg tac gag agc gtg atg cag cag cag gcg gcg gcg gcg 14070 Pro Pro Ser Tyr Glu Ser Val Met Gln Gln Gln Ala Ala Ala Ala 1630 1635 1640 atg atg cag ccc cca ctg gag gct ccc ttc gta ccc ccg cgg tac 14115 Met Met Gln Pro Pro Leu Glu Ala Pro Phe Val Pro Pro Arg Tyr 1645 1650 1655 ctg gcg cct acg gag ggg aga aac agc att cgt tac tcg gag ctg 14160 Leu Ala Pro Thr Glu Gly Arg Asn Ser Ile Arg Tyr Ser Glu Leu 1660 1665 1670 tca ccc cag tac gat acc acc aag ttg tat ctg gtg gac aac aag 14205 Ser Pro Gln Tyr Asp Thr Thr Lys Leu Tyr Leu Val Asp Asn Lys 1675 1680 1685 tcg gcg gac atc gcc tcc ctg aac tat cag aac gac cac agc aac 14250 Ser Ala Asp Ile Ala Ser Leu Asn Tyr Gln Asn Asp His Ser Asn 1690 1695 1700 ttc ctg acc acg gtg gtg cag aac aat gac ttt acc ccc acg gag 14295 Phe Leu Thr Thr Val Val Gln Asn Asn Asp Phe Thr Pro Thr Glu 1705 1710 1715 gcc agc acc cag acc atc aac ttt gac gag cgg tcg cgg tgg ggc 14340 Ala Ser Thr Gln Thr Ile Asn Phe Asp Glu Arg Ser Arg Trp Gly 1720 1725 1730 ggt cag ctg aag acc atc atg cac acc aac atg ccc aac gtg aac 14385 Gly Gln Leu Lys Thr Ile Met His Thr Asn Met Pro Asn Val Asn 1735 1740 1745 gag tac atg ttc agc aac aag ttc aag gcg cgg gtg atg gtg tcc 14430 Glu Tyr Met Phe Ser Asn Lys Phe Lys Ala Arg Val Met Val Ser 1750 1755 1760 aga aag gct cct gaa ggt gtt aca gta gat gac aaa tat gat cac 14475 Arg Lys Ala Pro Glu Gly Val Thr Val Asp Asp Lys Tyr Asp His 1765 1770 1775 aag caa gat att ctt aaa tat gag tgg ttt gag ttt aca ctg cca 14520 Lys Gln Asp Ile Leu Lys Tyr Glu Trp Phe Glu Phe Thr Leu Pro 1780 1785 1790 gaa ggc aac ttc tca gcc act atg aca att gat tta atg aac aat 14565 Glu Gly Asn Phe Ser Ala Thr Met Thr Ile Asp Leu Met Asn Asn 1795 1800 1805 gcc atc ata gac aac tac ctg gga gtt ggt aga cag aat gga gtc 14610 Ala Ile Ile Asp Asn Tyr Leu Gly Val Gly Arg Gln Asn Gly Val 1810 1815 1820 ctg gag agt gac att ggt gtc aag ttt gac act aga aac ttc agg 14655 Leu Glu Ser Asp Ile Gly Val Lys Phe Asp Thr Arg Asn Phe Arg 1825 1830 1835 ctc ggg tgg gac cca gaa act aag tta atc atg ccc ggg gtc tac 14700 Leu Gly Trp Asp Pro Glu Thr Lys Leu Ile Met Pro Gly Val Tyr 1840 1845 1850 acc tat gag gca ttc cat cct gac att gta ttg ctg cct ggt tgc 14745 Thr Tyr Glu Ala Phe His Pro Asp Ile Val Leu Leu Pro Gly Cys 1855 1860 1865 ggg gta gac ttt act gaa agc cgc ctt agt aac ttg ctt ggc atc 14790 Gly Val Asp Phe Thr Glu Ser Arg Leu Ser Asn Leu Leu Gly Ile 1870 1875 1880 agg aag aga cat cca ttc cag gag ggt ttc aaa atc atg tat gaa 14835 Arg Lys Arg His Pro Phe Gln Glu Gly Phe Lys Ile Met Tyr Glu 1885 1890 1895 gat ctt gaa ggg ggt aat att cct gcc ctt ttg gat gtt act gcc 14880 Asp Leu Glu Gly Gly Asn Ile Pro Ala Leu Leu Asp Val Thr Ala 1900 1905 1910 tat gag gaa agc aaa aag gat acc act act gaa aca ggc gaa aag 14925 Tyr Glu Glu Ser Lys Lys Asp Thr Thr Thr Glu Thr Gly Glu Lys 1915 1920 1925 gcg gtg gtt gaa agt gaa act gaa gcc atg act gaa aca acc aca 14970 Ala Val Val Glu Ser Glu Thr Glu Ala Met Thr Glu Thr Thr Thr 1930 1935 1940 ctg gct gtt gca gag gaa act agt gaa gat gat aat ata act aga 15015 Leu Ala Val Ala Glu Glu Thr Ser Glu Asp Asp Asn Ile Thr Arg 1945 1950 1955 gga gat act tat ata act gaa aaa caa aaa cgt gaa gct gca gct 15060 Gly Asp Thr Tyr Ile Thr Glu Lys Gln Lys Arg Glu Ala Ala Ala 1960 1965 1970 gca gag gca gaa cta tta ctt atg gct gaa gtt aaa aaa gag tta 15105 Ala Glu Ala Glu Leu Leu Leu Met Ala Glu Val Lys Lys Glu Leu 1975 1980 1985 aag atc caa cct tta gaa aaa gac agc aag agt aga agc tat aat 15150 Lys Ile Gln Pro Leu Glu Lys Asp Ser Lys Ser Arg Ser Tyr Asn 1990 1995 2000 gtc ttg gaa gac aaa atc aac aca gcc tac cgc agc tgg tac ctg 15195 Val Leu Glu Asp Lys Ile Asn Thr Ala Tyr Arg Ser Trp Tyr Leu 2005 2010 2015 tcc tac aat tat ggc gac cct gag aaa gga ata agg tcc tgg aca 15240 Ser Tyr Asn Tyr Gly Asp Pro Glu Lys Gly Ile Arg Ser Trp Thr 2020 2025 2030 ctg ctc acc acc tcg gat gtc acc tgc ggg gcg gag cag gtc tac 15285 Leu Leu Thr Thr Ser Asp Val Thr Cys Gly Ala Glu Gln Val Tyr 2035 2040 2045 tgg tcg ctc cca gac atg atg caa gac ccc gtc acc ttc cgc tcc 15330 Trp Ser Leu Pro Asp Met Met Gln Asp Pro Val Thr Phe Arg Ser 2050 2055 2060 acg aga caa gtc aac aac tac cca gtg gtg ggt gca gag ctt atg 15375 Thr Arg Gln Val Asn Asn Tyr Pro Val Val Gly Ala Glu Leu Met 2065 2070 2075 ccc gtc ttc tca aag agt ttc tac aac gag caa gcc gtg tac tcc 15420 Pro Val Phe Ser Lys Ser Phe Tyr Asn Glu Gln Ala Val Tyr Ser 2080 2085 2090 cag cag ctc cgc cag tcc acc tcg ctc acg cac gtc ttc aac cgc 15465 Gln Gln Leu Arg Gln Ser Thr Ser Leu Thr His Val Phe Asn Arg 2095 2100 2105 ttc cct gag aac cag atc ctc atc cgc ccg ccg gcg ccc aca att 15510 Phe Pro Glu Asn Gln Ile Leu Ile Arg Pro Pro Ala Pro Thr Ile 2110 2115 2120 acc acc gtc agt gaa aac gtt cct gct ctc aca gat cac ggg acc 15555 Thr Thr Val Ser Glu Asn Val Pro Ala Leu Thr Asp His Gly Thr 2125 2130 2135 ctg ccg ttg cgc agc agt atc cgg gga gtc cag cgc gtg acc gtt 15600 Leu Pro Leu Arg Ser Ser Ile Arg Gly Val Gln Arg Val Thr Val 2140 2145 2150 act gac gcc aga cgc cgc acc tgt ccc tac gtt tac aag gcc ctg 15645 Thr Asp Ala Arg Arg Arg Thr Cys Pro Tyr Val Tyr Lys Ala Leu 2155 2160 2165 ggc ata gtc gcg ccg cgc gtc ctt tca agc cgc act ttc taaaaaa 15691 Gly Ile Val Ala Pro Arg Val Leu Ser Ser Arg Thr Phe 2170 2175 2180 atg tcc att ctc atc tcg ccc agt aat aat acc ggt tgg gga ctg 15736 Met Ser Ile Leu Ile Ser Pro Ser Asn Asn Thr Gly Trp Gly Leu 2185 2190 2195 cgc gcg ccc acc aag atg tac gga ggc gcc cgc aaa cgc tct acc 15781 Arg Ala Pro Thr Lys Met Tyr Gly Gly Ala Arg Lys Arg Ser Thr 2200 2205 2210 cag cac cct gtg cgc gtg cgt ggt cat ttc cgc gct ccc tgg ggc 15826 Gln His Pro Val Arg Val Arg Gly His Phe Arg Ala Pro Trp Gly 2215 2220 2225 gcc ctc aag ggc cgt acc cgc act cgg acc acg gtc gat gat gtg 15871 Ala Leu Lys Gly Arg Thr Arg Thr Arg Thr Thr Val Asp Asp Val 2230 2235 2240 atc gac cag gtg gtc gcc gat gct cgt aat tat act cct act gcg 15916 Ile Asp Gln Val Val Ala Asp Ala Arg Asn Tyr Thr Pro Thr Ala 2245 2250 2255 cct aca tct act gtg gat gca gtt att gac agc gtg gtg gca aac 15961 Pro Thr Ser Thr Val Asp Ala Val Ile Asp Ser Val Val Ala Asn 2260 2265 2270 gcc cgc gcc tat gct cgt cgc aag agc cga agg agg cgc att gcc 16006 Ala Arg Ala Tyr Ala Arg Arg Lys Ser Arg Arg Arg Arg Ile Ala 2275 2280 2285 agg cgc cac cgg gct act ccc gcc atg cga gct gca aga gct ctt 16051 Arg Arg His Arg Ala Thr Pro Ala Met Arg Ala Ala Arg Ala Leu 2290 2295 2300 ctg cgg agg gcc aaa cgt gtg ggg cga aga gcc atg ctt aga gcg 16096 Leu Arg Arg Ala Lys Arg Val Gly Arg Arg Ala Met Leu Arg Ala 2305 2310 2315 gcc aga cgc gcg gct tca ggt gcc agc agc ggc agg tcc cgc agg 16141 Ala Arg Arg Ala Ala Ser Gly Ala Ser Ser Gly Arg Ser Arg Arg 2320 2325 2330 cgt gcg gcc acg gcg gca gca gcg gcc att gcc aac atg gcc caa 16186 Arg Ala Ala Thr Ala Ala Ala Ala Ala Ile Ala Asn Met Ala Gln 2335 2340 2345 ccg cga aga ggc aat gtg tac tgg gtg cgc gac gcc tct ggt cag 16231 Pro Arg Arg Gly Asn Val Tyr Trp Val Arg Asp Ala Ser Gly Gln 2350 2355 2360 cgc gtg ccc gtg cgc acc cgc ccc cct cgc act tgaagatact 16274 Arg Val Pro Val Arg Thr Arg Pro Pro Arg Thr 2365 2370 gagcagtctc cgatgttgtg tcccagcggc gagg atg tcc aag cgc aaa tac 16326 Met Ser Lys Arg Lys Tyr 2375 aag gaa gag atg ctc cag gtc atc gcg cct gaa atc tac ggt cca 16371 Lys Glu Glu Met Leu Gln Val Ile Ala Pro Glu Ile Tyr Gly Pro 2380 2385 2390 ccg gtg aag gat gaa aaa aag ccc cgc aaa atc aag cgg gtc aaa 16416 Pro Val Lys Asp Glu Lys Lys Pro Arg Lys Ile Lys Arg Val Lys 2395 2400 2405 aag gac aaa aag gaa gaa gat ggc gat gat ggg ctg gtg gag ttt 16461 Lys Asp Lys Lys Glu Glu Asp Gly Asp Asp Gly Leu Val Glu Phe 2410 2415 2420 gtg cgc gag ttc gcc cca agg cgg cgc gtg cag tgg cgc ggg cgc 16506 Val Arg Glu Phe Ala Pro Arg Arg Arg Val Gln Trp Arg Gly Arg 2425 2430 2435 aaa gtg cgt caa gtg ctg aga ccc ggg acc act gtg gtc ttc aca 16551 Lys Val Arg Gln Val Leu Arg Pro Gly Thr Thr Val Val Phe Thr 2440 2445 2450 cct ggc gag cgt tcc agc agt act ttt aag cgg tcc tat gat gag 16596 Pro Gly Glu Arg Ser Ser Ser Thr Phe Lys Arg Ser Tyr Asp Glu 2455 2460 2465 gtg tac ggg gat gac gat att ctt gag cag gcg gca gac cgc ctg 16641 Val Tyr Gly Asp Asp Asp Ile Leu Glu Gln Ala Ala Asp Arg Leu 2470 2475 2480 ggc gag ttt gct tat ggc aag cgc act cga tcc agt ccc aag gag 16686 Gly Glu Phe Ala Tyr Gly Lys Arg Thr Arg Ser Ser Pro Lys Glu 2485 2490 2495 gag gcg gtg tcc atc ccc ttg gat cat gga aat ccc acc ccc agc 16731 Glu Ala Val Ser Ile Pro Leu Asp His Gly Asn Pro Thr Pro Ser 2500 2505 2510 ctc aaa cca gtc acc ctg cag caa gtg ctg ccc gta cct ccg cgg 16776 Leu Lys Pro Val Thr Leu Gln Gln Val Leu Pro Val Pro Pro Arg 2515 2520 2525 aga ggc gtg aag cgc gag ggt gag gac ctg tat ccc acc atg cag 16821 Arg Gly Val Lys Arg Glu Gly Glu Asp Leu Tyr Pro Thr Met Gln 2530 2535 2540 cta atg gtg ccc aag cgc cag agg cta gaa gac gta ctg gag aaa 16866 Leu Met Val Pro Lys Arg Gln Arg Leu Glu Asp Val Leu Glu Lys 2545 2550 2555 atg aaa gtg gat ccc gat atc cag cct gag gtc aaa gtg aga ccc 16911 Met Lys Val Asp Pro Asp Ile Gln Pro Glu Val Lys Val Arg Pro 2560 2565 2570 atc aag gaa gtg gcg cca ggt ttg gga gta caa acc gtg gac atc 16956 Ile Lys Glu Val Ala Pro Gly Leu Gly Val Gln Thr Val Asp Ile 2575 2580 2585 aag att ccc acc gag tcc atg gaa gtg cag acc gaa cct gca aag 17001 Lys Ile Pro Thr Glu Ser Met Glu Val Gln Thr Glu Pro Ala Lys 2590 2595 2600 ccc aca gcc acc tcc att gag gtg cag acg gat ccc tgg atg ccc 17046 Pro Thr Ala Thr Ser Ile Glu Val Gln Thr Asp Pro Trp Met Pro 2605 2610 2615 gcg ccc gtt gcc gcc cac agc acc act cga aga ccc cgg cga aag 17091 Ala Pro Val Ala Ala His Ser Thr Thr Arg Arg Pro Arg Arg Lys 2620 2625 2630 tat ggc cca gca agt ctg cta atg ccc aac tat gct ctg cac cca 17136 Tyr Gly Pro Ala Ser Leu Leu Met Pro Asn Tyr Ala Leu His Pro 2635 2640 2645 tcc atc att ccc act ccg ggt tac cga ggc act cgc tac tac cgc 17181 Ser Ile Ile Pro Thr Pro Gly Tyr Arg Gly Thr Arg Tyr Tyr Arg 2650 2655 2660 agc cgg agc agc acc tcc cgc cgc cgc aaa aca cct gca agt cgc 17226 Ser Arg Ser Ser Thr Ser Arg Arg Arg Lys Thr Pro Ala Ser Arg 2665 2670 2675 act cgc cgt cgc cgc cgc cgc acc acc gcc agc aaa ctg acg ccc 17271 Thr Arg Arg Arg Arg Arg Arg Thr Thr Ala Ser Lys Leu Thr Pro 2680 2685 2690 gcc gcc ctg gtg cgg aga gtg tac cgc gat ggt cgc gct gaa cct 17316 Ala Ala Leu Val Arg Arg Val Tyr Arg Asp Gly Arg Ala Glu Pro 2695 2700 2705 ctg acg ctg ccg cgc gcg cgc tac cat ccg agc atc acc act 17358 Leu Thr Leu Pro Arg Ala Arg Tyr His Pro Ser Ile Thr Thr 2710 2715 2720 taatgactgt tgccgctgcc tccttgcaga t atg gcc ctc act tgc cgc ctt 17410 Met Ala Leu Thr Cys Arg Leu 2725 2730 cgc gtc ccc att act ggc tac cga gga aga aac tcg cgc cgt aga 17455 Arg Val Pro Ile Thr Gly Tyr Arg Gly Arg Asn Ser Arg Arg Arg 2735 2740 2745 agg atg ttg ggg cgc ggg atg cgt cgc cac aga cga agg cgc gct 17500 Arg Met Leu Gly Arg Gly Met Arg Arg His Arg Arg Arg Arg Ala 2750 2755 2760 atc agc aag cgg ttg ggg ggt ggc ttt ctg ccc gct cta att ccc 17545 Ile Ser Lys Arg Leu Gly Gly Gly Phe Leu Pro Ala Leu Ile Pro 2765 2770 2775 atc atc gcc gcg gcg atc ggg gct ata cca ggc ata gct tcc gtg 17590 Ile Ile Ala Ala Ala Ile Gly Ala Ile Pro Gly Ile Ala Ser Val 2780 2785 2790 gcg gtt cag gcc tcg cag cgc cac tgacattgga aaaacttata aataaaaata 17644 Ala Val Gln Ala Ser Gln Arg His 2795 gaatggactc tgacgctcct ggtcctgtga ctatgttttt gtagag atg gaa gac 17699 Met Glu Asp 2800 atc aat ttt tca tcc ctg gct ccg cga cac ggc acg agg ccg tac 17744 Ile Asn Phe Ser Ser Leu Ala Pro Arg His Gly Thr Arg Pro Tyr 2805 2810 2815 atg ggc acc tgg agc gac atc ggc acg agc caa ctg aac ggg ggc 17789 Met Gly Thr Trp Ser Asp Ile Gly Thr Ser Gln Leu Asn Gly Gly 2820 2825 2830 gcc ttc aat tgg agc agt atc tgg agc ggg ctt aaa aat ttt ggc 17834 Ala Phe Asn Trp Ser Ser Ile Trp Ser Gly Leu Lys Asn Phe Gly 2835 2840 2845 tcg acc ata aaa acc tat ggg aac aaa gct tgg aac agc agc aca 17879 Ser Thr Ile Lys Thr Tyr Gly Asn Lys Ala Trp Asn Ser Ser Thr 2850 2855 2860 ggg cag gct ctg aga aat aag ctt aaa gag cag aac ttc caa cag 17924 Gly Gln Ala Leu Arg Asn Lys Leu Lys Glu Gln Asn Phe Gln Gln 2865 2870 2875 aag gtg gtt gat ggg atc gcc tct ggt att aat ggc gta gtg gat 17969 Lys Val Val Asp Gly Ile Ala Ser Gly Ile Asn Gly Val Val Asp 2880 2885 2890 ctg gcc aac cag gct gtg cag aaa cag ata aac agc cgc ctg gac 18014 Leu Ala Asn Gln Ala Val Gln Lys Gln Ile Asn Ser Arg Leu Asp 2895 2900 2905 ccg ccg ccc gca gcc cct ggc gaa atg gaa gtt gag gaa gag ctc 18059 Pro Pro Pro Ala Ala Pro Gly Glu Met Glu Val Glu Glu Glu Leu 2910 2915 2920 cct ccg ctg gaa aag cgg ggc gac aag cgt ccg cgt ccc gat ctg 18104 Pro Pro Leu Glu Lys Arg Gly Asp Lys Arg Pro Arg Pro Asp Leu 2925 2930 2935 gag gag acg ctg gtg acg cgc gca gac gag ccc cct tca tac gag 18149 Glu Glu Thr Leu Val Thr Arg Ala Asp Glu Pro Pro Ser Tyr Glu 2940 2945 2950 gag gca gtg aag ctc gga atg ccc act acc aag cct ata gct ccc 18194 Glu Ala Val Lys Leu Gly Met Pro Thr Thr Lys Pro Ile Ala Pro 2955 2960 2965 atg gcc acc ggg gtg atg aaa cct tct cag tcg cat cgg ccc gcc 18239 Met Ala Thr Gly Val Met Lys Pro Ser Gln Ser His Arg Pro Ala 2970 2975 2980 acc ttg gac ttg cct cct ccc cct gct gct gca gcg cca gtt ccc 18284 Thr Leu Asp Leu Pro Pro Pro Pro Ala Ala Ala Ala Pro Val Pro 2985 2990 2995 aag cct gtc gct acc aga aag ccc acc gcc gca cag ccc gtc gcc 18329 Lys Pro Val Ala Thr Arg Lys Pro Thr Ala Ala Gln Pro Val Ala 3000 3005 3010 gta gcc aga ccg cga cct ggg ggc acg ccg cgc ccg aat gca aac 18374 Val Ala Arg Pro Arg Pro Gly Gly Thr Pro Arg Pro Asn Ala Asn 3015 3020 3025 tgg cag agt act ctg aac agc atc gtg ggt ctg ggc gta cag agt 18419 Trp Gln Ser Thr Leu Asn Ser Ile Val Gly Leu Gly Val Gln Ser 3030 3035 3040 gta aag cgc cgt cgc tgc ttt taattaaata tggagtagcg cttaacttgc 18470 Val Lys Arg Arg Arg Cys Phe 3045 ttgtctgtgt gtatgtatca tcaccacgcc gccgcagcag cagcagcaga ggagaaagga 18530 agaggtcgcg cgccgaggct gagttgcttt caag atg gcc acc cca tcg atg 18582 Met Ala Thr Pro Ser Met 3050 ctg ccc cag tgg gca tac atg cac atc gcc gga cag gat gct tcg 18627 Leu Pro Gln Trp Ala Tyr Met His Ile Ala Gly Gln Asp Ala Ser 3055 3060 3065 gag tac ctg agt ccg ggt ctg gtg cag ttc gcc cgt gcc aca gac 18672 Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala Arg Ala Thr Asp 3070 3075 3080 acc tac ttc aat ctg ggg aac aag ttt agg aac ccc acc gtg gcc 18717 Thr Tyr Phe Asn Leu Gly Asn Lys Phe Arg Asn Pro Thr Val Ala 3085 3090 3095 cct acc cac gat gtg acc acc gac cgt agc cag cgg ctg atg ctg 18762 Pro Thr His Asp Val Thr Thr Asp Arg Ser Gln Arg Leu Met Leu 3100 3105 3110 cgc ttt gtg ccc gtt gat cgg gag gac aat acc tac tct tac aaa 18807 Arg Phe Val Pro Val Asp Arg Glu Asp Asn Thr Tyr Ser Tyr Lys 3115 3120 3125 gtt cgc tac aca ctg gct gtg ggc gac aac aga gtg ctg gac atg 18852 Val Arg Tyr Thr Leu Ala Val Gly Asp Asn Arg Val Leu Asp Met 3130 3135 3140 gcc agc acc ttc ttt gac atc agg ggg gtg ctt gac aga ggt ccc 18897 Ala Ser Thr Phe Phe Asp Ile Arg Gly Val Leu Asp Arg Gly Pro 3145 3150 3155 agt ttc aag cca tac tct ggc aca gct tac aat tcc ctg gcg cct 18942 Ser Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser Leu Ala Pro 3160 3165 3170 aag ggc gcg ccc aat aca tgc cag tgg att gcc aag ggg gcg cct 18987 Lys Gly Ala Pro Asn Thr Cys Gln Trp Ile Ala Lys Gly Ala Pro 3175 3180 3185 gtt acc gat caa gac aat gaa gaa cag gaa tta aca gat gtt act 19032 Val Thr Asp Gln Asp Asn Glu Glu Gln Glu Leu Thr Asp Val Thr 3190 3195 3200 tac gct ttt ggc aat gct cca gta caa gca gaa gcc aaa att aca 19077 Tyr Ala Phe Gly Asn Ala Pro Val Gln Ala Glu Ala Lys Ile Thr 3205 3210 3215 aaa gat ggt ctg cca gta ggt ttg gaa att aca gaa gat gaa caa 19122 Lys Asp Gly Leu Pro Val Gly Leu Glu Ile Thr Glu Asp Glu Gln 3220 3225 3230 aag tca att tat gca gac aaa ttg tat cag cca gag ccc caa att 19167 Lys Ser Ile Tyr Ala Asp Lys Leu Tyr Gln Pro Glu Pro Gln Ile 3235 3240 3245 ggc gat gaa caa tgg cat gac acc act ggc act aat gaa caa tac 19212 Gly Asp Glu Gln Trp His Asp Thr Thr Gly Thr Asn Glu Gln Tyr 3250 3255 3260 ggc ggc aga gct cta aaa ccg gcc acc aac atg aaa cca tgt tat 19257 Gly Gly Arg Ala Leu Lys Pro Ala Thr Asn Met Lys Pro Cys Tyr 3265 3270 3275 ggc tca ttt gcc aga ccc aca aat aaa aaa ggc ggt cag gct aaa 19302 Gly Ser Phe Ala Arg Pro Thr Asn Lys Lys Gly Gly Gln Ala Lys 3280 3285 3290 act aga aaa ata gaa aag gaa gag aat gga gtt aaa acc gta act 19347 Thr Arg Lys Ile Glu Lys Glu Glu Asn Gly Val Lys Thr Val Thr 3295 3300 3305 gaa gaa gct gac att gat atg gac ttt tat gac tta aga tca caa 19392 Glu Glu Ala Asp Ile Asp Met Asp Phe Tyr Asp Leu Arg Ser Gln 3310 3315 3320 aga gca aat ttt gat cct aaa att gtt ctt tat tct gaa aat gta 19437 Arg Ala Asn Phe Asp Pro Lys Ile Val Leu Tyr Ser Glu Asn Val 3325 3330 3335 aat ttg gaa act cca gat aca cat att gtg tat aaa cca gga aca 19482 Asn Leu Glu Thr Pro Asp Thr His Ile Val Tyr Lys Pro Gly Thr 3340 3345 3350 gat gaa act agt tcc tct gtt aac ttg gga cag cag gca atg ccc 19527 Asp Glu Thr Ser Ser Ser Val Asn Leu Gly Gln Gln Ala Met Pro 3355 3360 3365 aac aga ccc aac tac att ggt ttt agg gac aac ttc att gga ctt 19572 Asn Arg Pro Asn Tyr Ile Gly Phe Arg Asp Asn Phe Ile Gly Leu 3370 3375 3380 atg ttt tac aac agt acc ggc aac atg ggc gtg ctg gcc ggg caa 19617 Met Phe Tyr Asn Ser Thr Gly Asn Met Gly Val Leu Ala Gly Gln 3385 3390 3395 gct tct cag tta aat gct gtg gtt gac ttg cag gac agg aac aca 19662 Ala Ser Gln Leu Asn Ala Val Val Asp Leu Gln Asp Arg Asn Thr 3400 3405 3410 gaa ctg tcc tac cag ctg ctg ctt gac tct ctg ggt gac aga acc 19707 Glu Leu Ser Tyr Gln Leu Leu Leu Asp Ser Leu Gly Asp Arg Thr 3415 3420 3425 aga tac ttt agc atg tgg aat cag gcc gtg gat agc tat gac cca 19752 Arg Tyr Phe Ser Met Trp Asn Gln Ala Val Asp Ser Tyr Asp Pro 3430 3435 3440 gac gtg cgc att att gaa aac cac ggt gtg gaa gac gaa ctt cct 19797 Asp Val Arg Ile Ile Glu Asn His Gly Val Glu Asp Glu Leu Pro 3445 3450 3455 aac tat tgt ttt cca tta gat gga gtg gga cca att acg ggc act 19842 Asn Tyr Cys Phe Pro Leu Asp Gly Val Gly Pro Ile Thr Gly Thr 3460 3465 3470 tat cag ggg gtt gag cct gat gga aac aat gga aac tgg aag aaa 19887 Tyr Gln Gly Val Glu Pro Asp Gly Asn Asn Gly Asn Trp Lys Lys 3475 3480 3485 aac aca aac ata aat gga gca aat gaa att ggc aag gga aat aac 19932 Asn Thr Asn Ile Asn Gly Ala Asn Glu Ile Gly Lys Gly Asn Asn 3490 3495 3500 tat gct atg gaa att aat cta caa gct aac ctc tgg aga agt ttt 19977 Tyr Ala Met Glu Ile Asn Leu Gln Ala Asn Leu Trp Arg Ser Phe 3505 3510 3515 cta tat tcc aat gtg gct ctg tat tta cca gac ggt tac aaa tat 20022 Leu Tyr Ser Asn Val Ala Leu Tyr Leu Pro Asp Gly Tyr Lys Tyr 3520 3525 3530 acc cca gcc aat gtt aca ctg cca gaa aac aaa aac acc tat ggc 20067 Thr Pro Ala Asn Val Thr Leu Pro Glu Asn Lys Asn Thr Tyr Gly 3535 3540 3545 tat ata aac gga cga gta gta tcc cca tct ttg gtg gat tca tac 20112 Tyr Ile Asn Gly Arg Val Val Ser Pro Ser Leu Val Asp Ser Tyr 3550 3555 3560 atc aac att gga gcc aga tgg tct ttg gat ctt atg gac aat gta 20157 Ile Asn Ile Gly Ala Arg Trp Ser Leu Asp Leu Met Asp Asn Val 3565 3570 3575 aac cca ttc aat cac cac cgc aat gca ggc ctg cgt tac cgt tcc 20202 Asn Pro Phe Asn His His Arg Asn Ala Gly Leu Arg Tyr Arg Ser 3580 3585 3590 atg ctt tta gga aat ggt cgc tat gtg cct ttc cac atc caa gtg 20247 Met Leu Leu Gly Asn Gly Arg Tyr Val Pro Phe His Ile Gln Val 3595 3600 3605 cct cag aaa ttc ttt gct gtc aag aac ctg ttg ctt ctt ccc ggc 20292 Pro Gln Lys Phe Phe Ala Val Lys Asn Leu Leu Leu Leu Pro Gly 3610 3615 3620 tcc tac acc tat gag tgg aac ttc aga aag gac gta aac atg gtc 20337 Ser Tyr Thr Tyr Glu Trp Asn Phe Arg Lys Asp Val Asn Met Val 3625 3630 3635 ctg caa agt tcc ctt ggt aat gat ctc aga act gat ggt gct agc 20382 Leu Gln Ser Ser Leu Gly Asn Asp Leu Arg Thr Asp Gly Ala Ser 3640 3645 3650 atc agt ttt acc agc atc aat cta tat gct acc ttt ttc ccc atg 20427 Ile Ser Phe Thr Ser Ile Asn Leu Tyr Ala Thr Phe Phe Pro Met 3655 3660 3665 gcc cac aac act gct tcc acc ctt gaa gcc atg ctg cgc aat gac 20472 Ala His Asn Thr Ala Ser Thr Leu Glu Ala Met Leu Arg Asn Asp 3670 3675 3680 acc aat gac cag tca ttt aat gac tac ctt tct gca gct aac atg 20517 Thr Asn Asp Gln Ser Phe Asn Asp Tyr Leu Ser Ala Ala Asn Met 3685 3690 3695 ctc tac cct att cca gcc aat gca acc aac atc ccc att tcc att 20562 Leu Tyr Pro Ile Pro Ala Asn Ala Thr Asn Ile Pro Ile Ser Ile 3700 3705 3710 ccc tct cgc aat tgg gcc gcc ttc agg ggc tgg tcc ttc acc aga 20607 Pro Ser Arg Asn Trp Ala Ala Phe Arg Gly Trp Ser Phe Thr Arg 3715 3720 3725 ctc aaa acc aag gag acc cca tct ctg gga tca ggg ttc gat ccc 20652 Leu Lys Thr Lys Glu Thr Pro Ser Leu Gly Ser Gly Phe Asp Pro 3730 3735 3740 tac ttt gtc tat tct ggt tct att ccc tac ctt gat ggc acc ttc 20697 Tyr Phe Val Tyr Ser Gly Ser Ile Pro Tyr Leu Asp Gly Thr Phe 3745 3750 3755 tac ctt aac cac act ttc aag aag gtc tcc atc atg ttt gac tcc 20742 Tyr Leu Asn His Thr Phe Lys Lys Val Ser Ile Met Phe Asp Ser 3760 3765 3770 tca gtc agc tgg cca ggc aat gac agg ctt cta act cca aat gag 20787 Ser Val Ser Trp Pro Gly Asn Asp Arg Leu Leu Thr Pro Asn Glu 3775 3780 3785 ttt gaa atc aaa cgc act gtg gat ggg gaa ggg tac aat gtg gct 20832 Phe Glu Ile Lys Arg Thr Val Asp Gly Glu Gly Tyr Asn Val Ala 3790 3795 3800 caa tgc aac atg acc aag gac tgg ttc ctg gtt caa atg ctc gcc 20877 Gln Cys Asn Met Thr Lys Asp Trp Phe Leu Val Gln Met Leu Ala 3805 3810 3815 aac tac aac att ggc tac cag ggc ttc tac atc cca gag ggg tac 20922 Asn Tyr Asn Ile Gly Tyr Gln Gly Phe Tyr Ile Pro Glu Gly Tyr 3820 3825 3830 aag gat cgc atg tac tcc ttc ttc aga aac ttc cag ccc atg agt 20967 Lys Asp Arg Met Tyr Ser Phe Phe Arg Asn Phe Gln Pro Met Ser 3835 3840 3845 agg cag gtg gtt gat gag atc aac tac aag gag tac caa gct gtc 21012 Arg Gln Val Val Asp Glu Ile Asn Tyr Lys Glu Tyr Gln Ala Val 3850 3855 3860 aca ctt gct tac cag cac aac aac tct ggc ttt gtg ggt tac cat 21057 Thr Leu Ala Tyr Gln His Asn Asn Ser Gly Phe Val Gly Tyr His 3865 3870 3875 gca ccc act ctc cgt cag ggt caa cca tac cca gct aac tac cca 21102 Ala Pro Thr Leu Arg Gln Gly Gln Pro Tyr Pro Ala Asn Tyr Pro 3880 3885 3890 tac ccg ctt att gga acc act gct gtc acc agc gtc acc cag aaa 21147 Tyr Pro Leu Ile Gly Thr Thr Ala Val Thr Ser Val Thr Gln Lys 3895 3900 3905 aag ttc ttg tgc gac agg acc atg tgg cgc atc ccc ttc tcc agc 21192 Lys Phe Leu Cys Asp Arg Thr Met Trp Arg Ile Pro Phe Ser Ser 3910 3915 3920 aac ttc atg tcc atg ggt gcc ctt acc gac ctg ggg cag aac atg 21237 Asn Phe Met Ser Met Gly Ala Leu Thr Asp Leu Gly Gln Asn Met 3925 3930 3935 ctt tat gct aac tca gct cat gcg ctg gac atg act ttt gag gtg 21282 Leu Tyr Ala Asn Ser Ala His Ala Leu Asp Met Thr Phe Glu Val 3940 3945 3950 gat ccc atg gat gag ccc aca ctg ctt tat ctt ctt ttc gaa gtc 21327 Asp Pro Met Asp Glu Pro Thr Leu Leu Tyr Leu Leu Phe Glu Val 3955 3960 3965 ttc gac gtg gtc aga gtg cac cag cca cac cgc ggc gtc atc gag 21372 Phe Asp Val Val Arg Val His Gln Pro His Arg Gly Val Ile Glu 3970 3975 3980 gcc gtc tac ctg cgc aca ccg ttc tcg gcc ggc aac gcc acc aca 21417 Ala Val Tyr Leu Arg Thr Pro Phe Ser Ala Gly Asn Ala Thr Thr 3985 3990 3995 taagaagcct cttgcttctt gcaagcagca gcagcagcc atg aca tgc ggg tcc 21471 Met Thr Cys Gly Ser 4000 gga aac ggc tcc agc gag caa gag ctc aaa gcc atc gtc cga gac 21516 Gly Asn Gly Ser Ser Glu Gln Glu Leu Lys Ala Ile Val Arg Asp 4005 4010 4015 ctg ggc tgc gga ccc tat ttc ctg gga acc ttt gac aag cgt ttc 21561 Leu Gly Cys Gly Pro Tyr Phe Leu Gly Thr Phe Asp Lys Arg Phe 4020 4025 4030 ccg ggg ttc atg gcc ccc gac aag ctc gcc tgc gcc ata gtc aac 21606 Pro Gly Phe Met Ala Pro Asp Lys Leu Ala Cys Ala Ile Val Asn 4035 4040 4045 act gcc ggc cgc gag acg ggg gga gag cac tgg ctg gct ttt ggt 21651 Thr Ala Gly Arg Glu Thr Gly Gly Glu His Trp Leu Ala Phe Gly 4050 4055 4060 tgg aac ccg cgc tcc aac acc tgc tac ctt ttt gat cct ttt ggg 21696 Trp Asn Pro Arg Ser Asn Thr Cys Tyr Leu Phe Asp Pro Phe Gly 4065 4070 4075 ttc tcg gat gag cgg ctc aaa cag att tac cag ttt gag tac gag 21741 Phe Ser Asp Glu Arg Leu Lys Gln Ile Tyr Gln Phe Glu Tyr Glu 4080 4085 4090 ggg ctc ctg cgc cgc agt gcc ctt gct acc aaa gac cgc tgc atc 21786 Gly Leu Leu Arg Arg Ser Ala Leu Ala Thr Lys Asp Arg Cys Ile 4095 4100 4105 acc ctg gaa aag tcc acc cag acc gtg cag ggc ccg cgc tca gcc 21831 Thr Leu Glu Lys Ser Thr Gln Thr Val Gln Gly Pro Arg Ser Ala 4110 4115 4120 gcc tgt gga ctt ttt tgc tgt atg ttc ctt cat gcc ttt gtg cac 21876 Ala Cys Gly Leu Phe Cys Cys Met Phe Leu His Ala Phe Val His 4125 4130 4135 tgg ccc gac cgc ccc atg gac gga aac ccc acc atg aag ttg ctg 21921 Trp Pro Asp Arg Pro Met Asp Gly Asn Pro Thr Met Lys Leu Leu 4140 4145 4150 act ggg gtg ccc aac agc atg ctc caa tca ccc caa gtc cag ccc 21966 Thr Gly Val Pro Asn Ser Met Leu Gln Ser Pro Gln Val Gln Pro 4155 4160 4165 acc ctg cgc cgc aac cag gag gcg cta tac cgc ttc cta aac acc 22011 Thr Leu Arg Arg Asn Gln Glu Ala Leu Tyr Arg Phe Leu Asn Thr 4170 4175 4180 cac tca tct tac ttt cgt tct cac cgc gcg cgc atc gaa agg gcc 22056 His Ser Ser Tyr Phe Arg Ser His Arg Ala Arg Ile Glu Arg Ala 4185 4190 4195 acc gcg ttt gac cgt atg gat atg caa taataagtca tgtaaaaacc 22103 Thr Ala Phe Asp Arg Met Asp Met Gln 4200 4205 gtgttcaata aacagcactt tatttttaca tgcactgagg ctctggtttg ctcattcatt 22163 catcattcac tcagaagtcg aatgggttct ggcgggagtc agagtgtccc gcgggcaggg 22223 atacgttgcg gaactggaac ctgttctgcc acttgaactc ggggatcacc agcttgggaa 22283 ctggaatctc ggggaaggtg tcttgccaca actttctggt cagttgcaaa gcgccaagca 22343 ggtcaggagc agagatcttg aaatcacagt tggggccggc attctggacg cgggagttgc 22403 ggtacactgg gttgcagcac tggaacacca tcaaggcggg gtgtctcacg cttgccagca 22463 cggtcgggtc actgatggta gtcacatcca agtcttcagc attggccatc ccaaagggtg 22523 tcatcttaca ggtctgcctg cccatcacgg gagcgcagcc gggcttgtgg ttgcaatcgc 22583 agtgaatggg gatcagcatc atcctggctt ggtcgggggt tatccctggg tacacggcct 22643 tcatgaaggc ttcgtactgc ttgaaagctt cctgggcctt acttccctcg gtgtagaaca 22703 tcccacagga cttgctggaa aattgattag tagcacagtt ggcatcattc acacagcagc 22763 gggcatcgtt gttggccagc tggaccacat ttctgcccca gcggttctgg gtgatcttgg 22823 ctcggtctgg gttctccttc atagcgcgct gcccgttctc gctcgccaca tccatctcga 22883 taatgtggtc cttctggatc atgatagtgc catgcaggca tttcaccttg ccttcataat 22943 cggtgcagcc atgagcccac agagcgcatc cggtgcactc ccaattattg tgggcgatct 23003 cagaataaga atgcaccaat ccctgcatga atcttcccat catcgctgtc agggtcttca 23063 tgctggtaaa ggtcaggggg atgccacggt gctcctcgtt cacatactgg tggcagatac 23123 gcttgtactg ctcgtgctgt tctggcatca gcttgaaaga ggttctcagg tcattatcca 23183 gcctgtacct ctccattagc acagccatta cttccatgcc cttctcccag gcagaaacca 23243 ggggaaggct catggaattt ctaacagaaa tagcagctac tttagccaga gggtcatcct 23303 tgtcaatctt ctcaacactt cttttgccat ccttctcagt gatgcgcacg ggtgggtagc 23363 tgaagcccac ggccaccagc tccgcctctt ctctttcttc ttcgctgtcc tgactgatgt 23423 cttgcagagg gacatgcttg gtcttcctgg gcttcttctt gggagggatc gggggagggc 23483 tgctgctccg ctccggagac agggaggacc gcgaagtttc gctcaccagt accacctggc 23543 tctcggtaga agaaccggac cccacgcggc ggtaggtgtt cctcttcggg ggcagaggtg 23603 gaggcgactg cgatgggctg cggtccggcc tgggaggcgg atggctggca gagcctcttc 23663 cgcgttcggg ggtgtgctcc cggtggcggt cgcttgactg atttcctccg cggctggcca 23723 ttgtgttctc ctaggcagag aaacaacaga c atg gag act cag cca tca ctg 23775 Met Glu Thr Gln Pro Ser Leu 4210 4215 cca aca tcg ctg caa gcg cca tca cac ctc gcc ccc agc agc gac 23820 Pro Thr Ser Leu Gln Ala Pro Ser His Leu Ala Pro Ser Ser Asp 4220 4225 4230 gag gag gag agc tta acc acc cca cca ccc agt ccc gcc acc acc 23865 Glu Glu Glu Ser Leu Thr Thr Pro Pro Pro Ser Pro Ala Thr Thr 4235 4240 4245 acc tct acc ctc gaa gat gag gag gag gtc gac gca ccc cag gag 23910 Thr Ser Thr Leu Glu Asp Glu Glu Glu Val Asp Ala Pro Gln Glu 4250 4255 4260 atg cag gcg cag gat atg gag gat gtg aaa gcg gaa gag att gag 23955 Met Gln Ala Gln Asp Met Glu Asp Val Lys Ala Glu Glu Ile Glu 4265 4270 4275 gca gat gtc gag cag gac ccg ggc tat gtg aca ccg gcg gag cac 24000 Ala Asp Val Glu Gln Asp Pro Gly Tyr Val Thr Pro Ala Glu His 4280 4285 4290 gag gag gag ctg aaa cgc ttt cta gac aga gag gaa gtt gac agc 24045 Glu Glu Glu Leu Lys Arg Phe Leu Asp Arg Glu Glu Val Asp Ser 4295 4300 4305 cgc cca gag cat caa gca gat ggc gat cac cag gag gct ggg ctc 24090 Arg Pro Glu His Gln Ala Asp Gly Asp His Gln Glu Ala Gly Leu 4310 4315 4320 ggg gat cat gtc gcc gac tac ctc acc ggg ctt ggc tca gag gac 24135 Gly Asp His Val Ala Asp Tyr Leu Thr Gly Leu Gly Ser Glu Asp 4325 4330 4335 gtg ctc ctc aag cat cta gca agg cag tcc atc ata gtc aaa gat 24180 Val Leu Leu Lys His Leu Ala Arg Gln Ser Ile Ile Val Lys Asp 4340 4345 4350 gca gtg ctc gac cgc acc gaa gtg ccc atc agt gtg gaa gag ctc 24225 Ala Val Leu Asp Arg Thr Glu Val Pro Ile Ser Val Glu Glu Leu 4355 4360 4365 agc cgc gcc tac gag ctc aac ctc ttt tcg cct cgg gtg ccc ccc 24270 Ser Arg Ala Tyr Glu Leu Asn Leu Phe Ser Pro Arg Val Pro Pro 4370 4375 4380 aag cgg cag cca aac ggc acc tgc gag ccc aac cct cgc ctt aac 24315 Lys Arg Gln Pro Asn Gly Thr Cys Glu Pro Asn Pro Arg Leu Asn 4385 4390 4395 ttc tat cca gct ttt act gtc ccc gaa gtg ctg gcc acc tac cac 24360 Phe Tyr Pro Ala Phe Thr Val Pro Glu Val Leu Ala Thr Tyr His 4400 4405 4410 atc ttt ttc aag aac caa aag att cca gtc tcc tgc cgc gcc aac 24405 Ile Phe Phe Lys Asn Gln Lys Ile Pro Val Ser Cys Arg Ala Asn 4415 4420 4425 cgc acc cgg gcc gat gcc ctg ctt aac ttg gga ccg ggc gct tgc 24450 Arg Thr Arg Ala Asp Ala Leu Leu Asn Leu Gly Pro Gly Ala Cys 4430 4435 4440 tta cct gat ata gct tcc ttg gaa gag gtt cca aag atc ttc gaa 24495 Leu Pro Asp Ile Ala Ser Leu Glu Glu Val Pro Lys Ile Phe Glu 4445 4450 4455 ggt ctg ggt agt gat gag act cgg gca gca aat gct ctg caa cag 24540 Gly Leu Gly Ser Asp Glu Thr Arg Ala Ala Asn Ala Leu Gln Gln 4460 4465 4470 gga gag aat ggc atg gat gaa cac cac agc gct ctg gtg gag ctg 24585 Gly Glu Asn Gly Met Asp Glu His His Ser Ala Leu Val Glu Leu 4475 4480 4485 gaa ggt gac aat gcc agg ctt gca gtg ctc aag cgc agt atc gtg 24630 Glu Gly Asp Asn Ala Arg Leu Ala Val Leu Lys Arg Ser Ile Val 4490 4495 4500 gtc acc cat ttt gcc tat ccc gct gtt aac ctg ccc ccc aaa gtc 24675 Val Thr His Phe Ala Tyr Pro Ala Val Asn Leu Pro Pro Lys Val 4505 4510 4515 atg agc gcg gtc atg gac cat ctg ctc atc aaa cga gca agt ccc 24720 Met Ser Ala Val Met Asp His Leu Leu Ile Lys Arg Ala Ser Pro 4520 4525 4530 ctt tca gaa gac cag aac atg cag gat cca gac gcc tcg gac gag 24765 Leu Ser Glu Asp Gln Asn Met Gln Asp Pro Asp Ala Ser Asp Glu 4535 4540 4545 ggc aag ccg gta gtc agt gac gag cag cta tct cgc tgg ctg ggt 24810 Gly Lys Pro Val Val Ser Asp Glu Gln Leu Ser Arg Trp Leu Gly 4550 4555 4560 acc aac tcc ccg cga gac ttg gaa gag agg cgc aag ctc atg atg 24855 Thr Asn Ser Pro Arg Asp Leu Glu Glu Arg Arg Lys Leu Met Met 4565 4570 4575 gct gta gtg cta gtg act gtg gag ctg gag tgt ctg cgc cgc ttt 24900 Ala Val Val Leu Val Thr Val Glu Leu Glu Cys Leu Arg Arg Phe 4580 4585 4590 ttc acc gac cct gag acc ctg cgc aag cta gag gag aac ctg cac 24945 Phe Thr Asp Pro Glu Thr Leu Arg Lys Leu Glu Glu Asn Leu His 4595 4600 4605 tac act ttt aga cat ggc ttc gtg cgc cag gca tgc aag att tcc 24990 Tyr Thr Phe Arg His Gly Phe Val Arg Gln Ala Cys Lys Ile Ser 4610 4615 4620 aac gtg gag ctc acc aac ctg gtt tcc tac atg ggc att ttg cat 25035 Asn Val Glu Leu Thr Asn Leu Val Ser Tyr Met Gly Ile Leu His 4625 4630 4635 gag aac cgc ctg ggg cag agc gtc ctg cac acc acc ctg aag ggg 25080 Glu Asn Arg Leu Gly Gln Ser Val Leu His Thr Thr Leu Lys Gly 4640 4645 4650 gag gcc cgc cgc gac tac atc cga gac tgt gtc tac ctc tac ctc 25125 Glu Ala Arg Arg Asp Tyr Ile Arg Asp Cys Val Tyr Leu Tyr Leu 4655 4660 4665 tgc cat acc tgg cag act ggc atg ggt gtg tgg caa cag tgt ttg 25170 Cys His Thr Trp Gln Thr Gly Met Gly Val Trp Gln Gln Cys Leu 4670 4675 4680 gaa gag cag aac cta aaa gag ctg gac aag ctc ttg cag aga tcc 25215 Glu Glu Gln Asn Leu Lys Glu Leu Asp Lys Leu Leu Gln Arg Ser 4685 4690 4695 ctc aaa gcc ctg tgg aca ggt ttt gac gag cgc acc gtc gcc tcg 25260 Leu Lys Ala Leu Trp Thr Gly Phe Asp Glu Arg Thr Val Ala Ser 4700 4705 4710 gac ctg gcg gac atc atc ttc ccc gag cgt ctc agg gtt act ctg 25305 Asp Leu Ala Asp Ile Ile Phe Pro Glu Arg Leu Arg Val Thr Leu 4715 4720 4725 cgc aac ggc ctg cct gac ttc atg agc cag agc atg ctt aac aac 25350 Arg Asn Gly Leu Pro Asp Phe Met Ser Gln Ser Met Leu Asn Asn 4730 4735 4740 ttt cgc tct ttc atc ctg gaa cgc tcc ggt atc ctg ccc gcc acc 25395 Phe Arg Ser Phe Ile Leu Glu Arg Ser Gly Ile Leu Pro Ala Thr 4745 4750 4755 tgc tgc gcg ctg ccc tcc gac ttt gtg cct ctt agc tac cga gag 25440 Cys Cys Ala Leu Pro Ser Asp Phe Val Pro Leu Ser Tyr Arg Glu 4760 4765 4770 tgc ccc ccg ccg cta tgg agc cac tgc tac ctg ttc cgc ctg gcc 25485 Cys Pro Pro Pro Leu Trp Ser His Cys Tyr Leu Phe Arg Leu Ala 4775 4780 4785 aac tac ctc tcc tac cac tcg gat gtg atc gag gat gtg agc gga 25530 Asn Tyr Leu Ser Tyr His Ser Asp Val Ile Glu Asp Val Ser Gly 4790 4795 4800 gac ggc ctg ctg gaa tgc cac tgc cgc tgc aat ctc tgc aca ccc 25575 Asp Gly Leu Leu Glu Cys His Cys Arg Cys Asn Leu Cys Thr Pro 4805 4810 4815 cac cgt tcc ctc gcc tgc aac ccc cag ttg ctg agc gag acc cag 25620 His Arg Ser Leu Ala Cys Asn Pro Gln Leu Leu Ser Glu Thr Gln 4820 4825 4830 atc atc ggc acc ttc gag ttg cag ggt ccc agc agt gaa ggc gag 25665 Ile Ile Gly Thr Phe Glu Leu Gln Gly Pro Ser Ser Glu Gly Glu 4835 4840 4845 ggg tct tct ccg ggg cag agt ctg aaa ctg aca ccg ggg ctg tgg 25710 Gly Ser Ser Pro Gly Gln Ser Leu Lys Leu Thr Pro Gly Leu Trp 4850 4855 4860 acc tcc gcc tac ctg cgc aag ttt cac ccc gag gac tac cac ccc 25755 Thr Ser Ala Tyr Leu Arg Lys Phe His Pro Glu Asp Tyr His Pro 4865 4870 4875 tat gag atc agg ttc tat gag gac caa tca cat cct ccc aaa gtc 25800 Tyr Glu Ile Arg Phe Tyr Glu Asp Gln Ser His Pro Pro Lys Val 4880 4885 4890 gag ctc tca gcc tgc gtc atc acc cag ggg gca att ctg gcc caa 25845 Glu Leu Ser Ala Cys Val Ile Thr Gln Gly Ala Ile Leu Ala Gln 4895 4900 4905 ttg caa gcc atc caa aaa tcc cgc caa gaa ttt ctg atg aaa aag 25890 Leu Gln Ala Ile Gln Lys Ser Arg Gln Glu Phe Leu Met Lys Lys 4910 4915 4920 ggg agc ggg gtc tac ctc gac ccc cag acc ggt gag gag ctc aac 25935 Gly Ser Gly Val Tyr Leu Asp Pro Gln Thr Gly Glu Glu Leu Asn 4925 4930 4935 aca agg ttc ccc cag gat gtc cca gcg ccg agg aag caa gaa gct 25980 Thr Arg Phe Pro Gln Asp Val Pro Ala Pro Arg Lys Gln Glu Ala 4940 4945 4950 gaa ggt gca gct gcc gcc ccc aga gga tat gga gga aga ctg gga 26025 Glu Gly Ala Ala Ala Ala Pro Arg Gly Tyr Gly Gly Arg Leu Gly 4955 4960 4965 cag tca ggc aga gga agc gga gga gat gga aga ttg gga cag cca 26070 Gln Ser Gly Arg Gly Ser Gly Gly Asp Gly Arg Leu Gly Gln Pro 4970 4975 4980 ggc aga gga ggt gga cag cct gga gga aga cag ttt gga gga gga 26115 Gly Arg Gly Gly Gly Gln Pro Gly Gly Arg Gln Phe Gly Gly Gly 4985 4990 4995 aga cga gga ggc aga gga ggt gga aga agc aac cgc cgc caa aca 26160 Arg Arg Gly Gly Arg Gly Gly Gly Arg Ser Asn Arg Arg Gln Thr 5000 5005 5010 gtt gtc ctc ggc ggc gga gac aag caa gtc ccc aga cag cag cac 26205 Val Val Leu Gly Gly Gly Asp Lys Gln Val Pro Arg Gln Gln His 5015 5020 5025 ggc tac cat ctc cgc tcc ggg tcg ggg ggc cca gcg gcg gcc caa 26250 Gly Tyr His Leu Arg Ser Gly Ser Gly Gly Pro Ala Ala Ala Gln 5030 5035 5040 cag tagatgggac gagaccgggc gcttcccgaa cccgaccacc gcttccaaga 26303 Gln ccggtaagaa ggagcgacag ggatacaagt cctggcgggg acataaaaac gctatcatct 26363 cctgcttgca tgaatgcggg ggcaacatat ccttcacccg gcgctacctg ctcttccacc 26423 acggggtgaa cttcccccgc aatatcttgc attactaccg tcacctccac agcccctact 26483 gcagccagca agccccggca acctcggcag aaaaagacag cagcggcaac ggggaccaga 26543 aaaccagcag ttagaaaatc cacagcaagt gcagcaggag gaggactgag gatcacagcg 26603 aacgagccag cgcagaccag agagctgagg aatcggatct ttccaaccct ctatgccatc 26663 ttccagcaga gtcgggggca agagcaggaa ctgaaagtaa aaaaccgatc tctgcgctcg 26723 ctcaccagaa gttgtttgta tcacaagagc gaagaccaac ttcagcgcac tctcgaggac 26783 gccgaggctc tcttcaacaa gtactgcgcg ctgactctta aagagtagcc cttgcccgcg 26843 ctcgctcgaa aaaggcggga atcacgtcac ccttggcacc tgtcctttgc cctcgtc 26900 atg agt aaa gaa att ccc acg cct tac atg tgg agc tat cag ccc 26945 Met Ser Lys Glu Ile Pro Thr Pro Tyr Met Trp Ser Tyr Gln Pro 5045 5050 5055 caa atg ggg ttg gca gca ggc gcc tcc cag gac tac tcc acc cgc 26990 Gln Met Gly Leu Ala Ala Gly Ala Ser Gln Asp Tyr Ser Thr Arg 5060 5065 5070 atg aat tgg ctc agc gcc ggg ccc tcg atg atc tca cgg gtt aat 27035 Met Asn Trp Leu Ser Ala Gly Pro Ser Met Ile Ser Arg Val Asn 5075 5080 5085 gat ata cta gct tat cga aac cag tta ctc cta gaa cag tca gct 27080 Asp Ile Leu Ala Tyr Arg Asn Gln Leu Leu Leu Glu Gln Ser Ala 5090 5095 5100 ctc acc acc aca ccc cgc caa cac ctt aat ccc cgg aat tgg ccc 27125 Leu Thr Thr Thr Pro Arg Gln His Leu Asn Pro Arg Asn Trp Pro 5105 5110 5115 gcc gcc ctg gtg tac cag gaa act ccc gct ccc acc acc gta cta 27170 Ala Ala Leu Val Tyr Gln Glu Thr Pro Ala Pro Thr Thr Val Leu 5120 5125 5130 ctt cct cga gac gcc cag gcc gaa gtt cag atg act aac gca ggt 27215 Leu Pro Arg Asp Ala Gln Ala Glu Val Gln Met Thr Asn Ala Gly 5135 5140 5145 gta cag ctg gcg ggc ggt tcc gcc ctg tgt cgt cac cgg cct cgg 27260 Val Gln Leu Ala Gly Gly Ser Ala Leu Cys Arg His Arg Pro Arg 5150 5155 5160 cag agt ata aaa cgc ctg gtg atc aga ggc cga ggt atc cag ctc 27305 Gln Ser Ile Lys Arg Leu Val Ile Arg Gly Arg Gly Ile Gln Leu 5165 5170 5175 aac gac gag tcg gtg agc tct tcg ctt ggt ctg cga cca gac gga 27350 Asn Asp Glu Ser Val Ser Ser Ser Leu Gly Leu Arg Pro Asp Gly 5180 5185 5190 gtc ttc cag atc gcc ggc tgt gga aga tct tcc ttc act cct cgt 27395 Val Phe Gln Ile Ala Gly Cys Gly Arg Ser Ser Phe Thr Pro Arg 5195 5200 5205 cag gct gtg ctg act ttg gag agt tcg tcc tcg cag ccc cgc tcg 27440 Gln Ala Val Leu Thr Leu Glu Ser Ser Ser Ser Gln Pro Arg Ser 5210 5215 5220 ggc ggc atc ggg act ctc cag ttc gtg gag gag ttt act ccc tct 27485 Gly Gly Ile Gly Thr Leu Gln Phe Val Glu Glu Phe Thr Pro Ser 5225 5230 5235 gtg tac ttc aac ccc ttc tcc ggc tct cct ggc cag tac ccg gac 27530 Val Tyr Phe Asn Pro Phe Ser Gly Ser Pro Gly Gln Tyr Pro Asp 5240 5245 5250 gag ttc ata ccg aac ttc gac gca atc agc gag tca gtg gat ggc 27575 Glu Phe Ile Pro Asn Phe Asp Ala Ile Ser Glu Ser Val Asp Gly 5255 5260 5265 tat gat tg atg tct aat ggt ggc gcg gct gag cta gct cga ctg cga 27622 Tyr Asp Met Ser Asn Gly Gly Ala Ala Glu Leu Ala Arg Leu Arg 5270 5275 5280 cat cta gac cac tgc cgc cgc ttt cgc tgc ttc gcc cgg gaa ctc 27667 His Leu Asp His Cys Arg Arg Phe Arg Cys Phe Ala Arg Glu Leu 5285 5290 5295 acc gag ttc atc tac ttc gaa ctc ccc gag gag cac cct cag ggg 27712 Thr Glu Phe Ile Tyr Phe Glu Leu Pro Glu Glu His Pro Gln Gly 5300 5305 5310 ccg gcc cac gga gtg cgg att acc atc gaa ggg gga ata gac tct 27757 Pro Ala His Gly Val Arg Ile Thr Ile Glu Gly Gly Ile Asp Ser 5315 5320 5325 cgc ctg cat cgg atc ttc tcc cag cga ccc gtg ctg atc gag cgc 27802 Arg Leu His Arg Ile Phe Ser Gln Arg Pro Val Leu Ile Glu Arg 5330 5335 5340 gac cag gga aat aca acc atc tcc atc tac tgc atc tgt aac cac 27847 Asp Gln Gly Asn Thr Thr Ile Ser Ile Tyr Cys Ile Cys Asn His 5345 5350 5355 ccc gga ttg cat gaa agc ctt tgc tgt ctt att tgt gct gag ttt 27892 Pro Gly Leu His Glu Ser Leu Cys Cys Leu Ile Cys Ala Glu Phe 5360 5365 5370 aat aaa aac tgagttaaga ccctcctacg gactaccgct tcttcaacca 27941 Asn Lys Asn ggactttaca acaacaccaa ccagaccctc cgttccagcc agaagaccca gacccttcct 28001 cctctgatcc aggactctaa ctctaccttc ccagcaccat cccctactaa ccttcccgaa 28061 actaacaacc tcggagctca actgcaacac cgcctttccc gaagcctcct ttctgccaat 28121 actaccactc ccaaaaccgg aggtgagctc cgcggtctcc ccactgacga cccctgggtg 28181 gtagcgggtt ttgtaacgtt aggagtagtt gcgggtgggc ttgtgctgat cctttgctac 28241 ctatacacac cttgctgtgc atatttagtt atattgtgct gctggtttaa gaa atg 28297 Met 5375 ggg acc cta cta gtc gtg ctt gct tta ctt tcg ctt ttg gga ctg 28342 Gly Thr Leu Leu Val Val Leu Ala Leu Leu Ser Leu Leu Gly Leu 5380 5385 5390 ggc tct gct aat ctc att cct cct gat cac gat cca tgt gtg aca 28387 Gly Ser Ala Asn Leu Ile Pro Pro Asp His Asp Pro Cys Val Thr 5395 5400 5405 ttt gat cca gaa aac tgc aca ctc acc ttt gca cct gaa aca agc 28432 Phe Asp Pro Glu Asn Cys Thr Leu Thr Phe Ala Pro Glu Thr Ser 5410 5415 5420 cgc tac tgc gga gta gtt att agg tgc gga ctg gaa tgc agg ccc 28477 Arg Tyr Cys Gly Val Val Ile Arg Cys Gly Leu Glu Cys Arg Pro 5425 5430 5435 att gaa att aca cac aat aac aaa act tgg aac aat aca tta ttc 28522 Ile Glu Ile Thr His Asn Asn Lys Thr Trp Asn Asn Thr Leu Phe 5440 5445 5450 acc aca tgg caa cca gga tat cct cag tgg tat act gtc tct gtc 28567 Thr Thr Trp Gln Pro Gly Tyr Pro Gln Trp Tyr Thr Val Ser Val 5455 5460 5465 cgg ggt cct gac ggt tcc gtc cgc atg gct aat aac act ttc att 28612 Arg Gly Pro Asp Gly Ser Val Arg Met Ala Asn Asn Thr Phe Ile 5470 5475 5480 ttt gct gaa atg tgc gat atg gtc atg ttt atg agc aga cag tat 28657 Phe Ala Glu Met Cys Asp Met Val Met Phe Met Ser Arg Gln Tyr 5485 5490 5495 gac cta tgg cct ccc agc aaa gaa aac att gtg gca ttc tcc att 28702 Asp Leu Trp Pro Pro Ser Lys Glu Asn Ile Val Ala Phe Ser Ile 5500 5505 5510 gtt tat tgc ttg gga aca tgc atc atc act gct atc gtg tgt gtg 28747 Val Tyr Cys Leu Gly Thr Cys Ile Ile Thr Ala Ile Val Cys Val 5515 5520 5525 tgc ata cac ttg ctt ata gtc att cgc ccc aga aac agc aat gag 28792 Cys Ile His Leu Leu Ile Val Ile Arg Pro Arg Asn Ser Asn Glu 5530 5535 5540 gaa aaa gag aaa atg ccc taactttttt cacaactttt tttcagccat 28840 Glu Lys Glu Lys Met Pro 5545 gccttcagct ttttttcttc ttactattgt tgctgttatt tccgcacaaa caatagtaga 28900 tgttccactt ggttctaact acacactaat aggtcctaca atccattcag aagttacctg 28960 gtgcaggctt aatactgaag actactataa tgtattttgt gatggggatg atgacattca 29020 agtaacctgt aacaaacaga atcttacact cattaatgtt accaaaagtt acaatggtta 29080 ctattatgga tatgatagat ctggcagtga atttaaaaat tacctggtac gaacaattcc 29140 acccattaca aacattaaaa tagagaaact ccaaatggat agtgacattt taagtaatct 29200 tacaatatcc cccaccacac catctgaaca aaacattcca agttcaatga ttgcaattat 29260 tgcggcggtg gcagtgggaa tggcaatcat aataacatgt atgattgttt atgcttgctg 29320 ctacaagaaa atcaggcgtg aaaaacaaga ttcactacta aattatgatt tttaacttct 29380 tattttaaca gaca atg att ttc att aca gtt ctt ctt gcc atc ttt aac 29430 Met Ile Phe Ile Thr Val Leu Leu Ala Ile Phe Asn 5550 5555 tta cta tca gcc tct cat ggg cgc aca cat gtc act cta act act 29475 Leu Leu Ser Ala Ser His Gly Arg Thr His Val Thr Leu Thr Thr 5560 5565 5570 ggt tcc aca tac aca cta aaa ggc cca gaa ggt cat aat ggt gtt 29520 Gly Ser Thr Tyr Thr Leu Lys Gly Pro Glu Gly His Asn Gly Val 5575 5580 5585 att tgg tgg aaa cta ttt gat gat gga ggg ttt gtt agt ccc tgc 29565 Ile Trp Trp Lys Leu Phe Asp Asp Gly Gly Phe Val Ser Pro Cys 5590 5595 5600 agc aca tct aat aga tat tta tgt aat ggt aaa gac cta act att 29610 Ser Thr Ser Asn Arg Tyr Leu Cys Asn Gly Lys Asp Leu Thr Ile 5605 5610 5615 att aat gtc aca aaa cac gac aat ggc tac tat tat ggg acc aat 29655 Ile Asn Val Thr Lys His Asp Asn Gly Tyr Tyr Tyr Gly Thr Asn 5620 5625 5630 tat att aca agt tta gat tac acc att act gtc ata tcg cct act 29700 Tyr Ile Thr Ser Leu Asp Tyr Thr Ile Thr Val Ile Ser Pro Thr 5635 5640 5645 aca cca gca ccg cgc aaa atc aca act ttc tct agc agc agc gct 29745 Thr Pro Ala Pro Arg Lys Ile Thr Thr Phe Ser Ser Ser Ser Ala 5650 5655 5660 aaa aac aca atc aaa att aat aca act gct ata aaa atg ctc caa 29790 Lys Asn Thr Ile Lys Ile Asn Thr Thr Ala Ile Lys Met Leu Gln 5665 5670 5675 aaa atg gct tct aat tat acc cca ccc gct acc aat gcg ctt cct 29835 Lys Met Ala Ser Asn Tyr Thr Pro Pro Ala Thr Asn Ala Leu Pro 5680 5685 5690 aaa tca att att gga ata att gta gcg gcg gta gtg ggg ctg gca 29880 Lys Ser Ile Ile Gly Ile Ile Val Ala Ala Val Val Gly Leu Ala 5695 5700 5705 att att att tct tgc ata att tat tat gcc tgc tgc tat aga aaa 29925 Ile Ile Ile Ser Cys Ile Ile Tyr Tyr Ala Cys Cys Tyr Arg Lys 5710 5715 5720 ata aaa gga gac ccc cta cta agc ttt gat att taattttttt 29968 Ile Lys Gly Asp Pro Leu Leu Ser Phe Asp Ile 5725 5730 tcatagcacc atg aaa ttc cta tgt gta tta gct ttt tca gtt ttt agc 30017 Met Lys Phe Leu Cys Val Leu Ala Phe Ser Val Phe Ser 5735 5740 5745 ttt tgc aca tcc acc ccc atc acc att gtc aat gtg cag act act 30062 Phe Cys Thr Ser Thr Pro Ile Thr Ile Val Asn Val Gln Thr Thr 5750 5755 5760 tta aat cat gtt aat act aca aat tac aca tct acc tcc tat gca 30107 Leu Asn His Val Asn Thr Thr Asn Tyr Thr Ser Thr Ser Tyr Ala 5765 5770 5775 acc ata cat acc cag ctt att cct ttt tcc aca att aaa gcc aat 30152 Thr Ile His Thr Gln Leu Ile Pro Phe Ser Thr Ile Lys Ala Asn 5780 5785 5790 cct cag act aaa ttt gca cta caa cta gaa atc act atc cta att 30197 Pro Gln Thr Lys Phe Ala Leu Gln Leu Glu Ile Thr Ile Leu Ile 5795 5800 5805 gtg att gga ata act att cta gct gtt ctt ctt tat ttt ata ttc 30242 Val Ile Gly Ile Thr Ile Leu Ala Val Leu Leu Tyr Phe Ile Phe 5810 5815 5820 tgc cgc caa ata ccc aat gtt cat aaa aaa cca aaa aga caa ccc 30287 Cys Arg Gln Ile Pro Asn Val His Lys Lys Pro Lys Arg Gln Pro 5825 5830 5835 att tat tgt cct atg att agt aaa cct cac ttg gcc tta aat gaa 30332 Ile Tyr Cys Pro Met Ile Ser Lys Pro His Leu Ala Leu Asn Glu 5840 5845 5850 atc taaggtctat tcttttcttt tttacagtat ggtgatcacc aatc atg atc cct 30388 Ile Met Ile Pro 5855 aga aat ttc ttc ttc acc ata ctc atc tgt gct ttc aat gtc tgt 30433 Arg Asn Phe Phe Phe Thr Ile Leu Ile Cys Ala Phe Asn Val Cys 5860 5865 5870 gct acc ttc acc gca gta gcc act gca acc cca gac tgt ata gga 30478 Ala Thr Phe Thr Ala Val Ala Thr Ala Thr Pro Asp Cys Ile Gly 5875 5880 5885 gca ttt gct tcc tat gta ctt ttt gcc ttt gtt act tgc atc tgc 30523 Ala Phe Ala Ser Tyr Val Leu Phe Ala Phe Val Thr Cys Ile Cys 5890 5895 5900 gtg tgt agc ata gtc tgc ctg gtt att aat ttt ttc caa ctt gta 30568 Val Cys Ser Ile Val Cys Leu Val Ile Asn Phe Phe Gln Leu Val 5905 5910 5915 gac tgg atc ttt gta cga att gcc tac ctg cgt cac cat ccc gaa 30613 Asp Trp Ile Phe Val Arg Ile Ala Tyr Leu Arg His His Pro Glu 5920 5925 5930 tac cgc aat caa aat gtt gcg gca ctt ctt agg ctt att taaaaccatg 30662 Tyr Arg Asn Gln Asn Val Ala Ala Leu Leu Arg Leu Ile 5935 5940 caggctatgc taccagtcat tctaattctg ctgctaccct gcgttgcctt agctcccaca 30722 accactcgca ctccacctga acaacttaga aaatgcaaat ttcaacaacc atggtcattc 30782 cttgattgct accatgaaaa atctgatttt cccacatact ggatagtgat tgttggaata 30842 attaacatac tctcatgtac cttattctca ttcctaatat accccatatt tagttttggg 30902 tggaatgctc ccaatgcact gggttaccca caaattccag aggaacacat tgcactacag 30962 aacatgcaac agccactaga tctaatagat tatgaaaatg agccacagcc tccactactc 31022 cctgccatta gctacttcaa cctaaccggt ggag atg act gat cag ctc aac 31074 Met Thr Asp Gln Leu Asn 5945 5950 gcc tcc act gct gcc gtg gat ctg ctt gac atg gat ggc cgt acc 31119 Ala Ser Thr Ala Ala Val Asp Leu Leu Asp Met Asp Gly Arg Thr 5955 5960 5965 tca gaa cag cgt ctc gcc caa cta cgc ata cgt cag caa cag gaa 31164 Ser Glu Gln Arg Leu Ala Gln Leu Arg Ile Arg Gln Gln Gln Glu 5970 5975 5980 cgt gcc gcc aag gag ctc agg aat gcc atc gag att cac cag tgt 31209 Arg Ala Ala Lys Glu Leu Arg Asn Ala Ile Glu Ile His Gln Cys 5985 5990 5995 aaa aaa gga atc ttc tgc ttg gta aaa caa gcc aag att tcc tac 31254 Lys Lys Gly Ile Phe Cys Leu Val Lys Gln Ala Lys Ile Ser Tyr 6000 6005 6010 gag atc act gct aat gac cac cgt ctg tca tat gag ctt gtt cag 31299 Glu Ile Thr Ala Asn Asp His Arg Leu Ser Tyr Glu Leu Val Gln 6015 6020 6025 cag cga cag aaa ttc act tgc atg gtg gga atc aac ccc ata gta 31344 Gln Arg Gln Lys Phe Thr Cys Met Val Gly Ile Asn Pro Ile Val 6030 6035 6040 atc acc cag caa tct gga gat acc aag ggc tgt atc cat tgt tcc 31389 Ile Thr Gln Gln Ser Gly Asp Thr Lys Gly Cys Ile His Cys Ser 6045 6050 6055 tgt gac tcc acc gag tgc atc tac acc ctg ctg aag acc ctc tgc 31434 Cys Asp Ser Thr Glu Cys Ile Tyr Thr Leu Leu Lys Thr Leu Cys 6060 6065 6070 ggc ctt cga gac ctc cta ccc atg aac taatcaatgg cccttcccca 31481 Gly Leu Arg Asp Leu Leu Pro Met Asn 6075 attccaaaat agcaattaaa attatcaata aagatcactt acttgaaatc agcaataagg 31541 tttctgtcaa aattttctcc cagcagcacc tcactcccct cttcccaact ctggtactct 31601 aaacctcggc gggcggcata ctttctccac actttgaaag ggatgtcaaa ttttatttcc 31661 tcttctttgc ccacaatctt catttcttta tccccag atg gcc aaa cgg gct 31713 Met Ala Lys Arg Ala 6080 cgt cta agc agc tcc ttc aac ccg gtg tac ccc tat gaa gac gag 31758 Arg Leu Ser Ser Ser Phe Asn Pro Val Tyr Pro Tyr Glu Asp Glu 6085 6090 6095 agc agc tca caa cac cca ttt ata aac ccc ggc ttc att tcc cct 31803 Ser Ser Ser Gln His Pro Phe Ile Asn Pro Gly Phe Ile Ser Pro 6100 6105 6110 gat ggc ttt aca caa agc cca gac gga gtt cta aca ctg aaa tgt 31848 Asp Gly Phe Thr Gln Ser Pro Asp Gly Val Leu Thr Leu Lys Cys 6115 6120 6125 gtt tcc cct ctt act acc acc agt ggc gct cta gac att aaa gtg 31893 Val Ser Pro Leu Thr Thr Thr Ser Gly Ala Leu Asp Ile Lys Val 6130 6135 6140 gga aga ggg ctt aaa gta gat agc act gat ggt tcc ctg gaa gaa 31938 Gly Arg Gly Leu Lys Val Asp Ser Thr Asp Gly Ser Leu Glu Glu 6145 6150 6155 aat ata gac att aca gct ccc ctc act aaa ttt aac cac tca gta 31983 Asn Ile Asp Ile Thr Ala Pro Leu Thr Lys Phe Asn His Ser Val 6160 6165 6170 gga tta gca ttt ggc gac ggt cta gaa aca aaa gaa aac aag ctt 32028 Gly Leu Ala Phe Gly Asp Gly Leu Glu Thr Lys Glu Asn Lys Leu 6175 6180 6185 tat gta aaa ctt gga gat gga ctt aaa ttt agc tct ggc agt ata 32073 Tyr Val Lys Leu Gly Asp Gly Leu Lys Phe Ser Ser Gly Ser Ile 6190 6195 6200 tac att gac cat gat gtt aac act tta tgg aca gga gtc aat cca 32118 Tyr Ile Asp His Asp Val Asn Thr Leu Trp Thr Gly Val Asn Pro 6205 6210 6215 agt gct aac tgt ata att aca gac aat gga gaa acc aat gac agc 32163 Ser Ala Asn Cys Ile Ile Thr Asp Asn Gly Glu Thr Asn Asp Ser 6220 6225 6230 aag ctt acc cta ata ctt gtt aag tca ggt gga tta ata aat gct 32208 Lys Leu Thr Leu Ile Leu Val Lys Ser Gly Gly Leu Ile Asn Ala 6235 6240 6245 tat gtc tca tta atg ggt gac tca gac aca gtc aat aaa tta acc 32253 Tyr Val Ser Leu Met Gly Asp Ser Asp Thr Val Asn Lys Leu Thr 6250 6255 6260 aca gaa aaa agt gct caa att acc gtt gac ata tac ttt gat aat 32298 Thr Glu Lys Ser Ala Gln Ile Thr Val Asp Ile Tyr Phe Asp Asn 6265 6270 6275 caa gga aaa gtt ctt act gaa cta tcg gcc ctt aaa aca gat ctt 32343 Gln Gly Lys Val Leu Thr Glu Leu Ser Ala Leu Lys Thr Asp Leu 6280 6285 6290 aaa cat aaa ttt ggt caa aac atg gct tct agc gaa gta tca aac 32388 Lys His Lys Phe Gly Gln Asn Met Ala Ser Ser Glu Val Ser Asn 6295 6300 6305 tgc aaa ggc ttt atg cca agc tta aat gca tac cca ttc aga aat 32433 Cys Lys Gly Phe Met Pro Ser Leu Asn Ala Tyr Pro Phe Arg Asn 6310 6315 6320 cca act aaa cct acc aaa gga aga gaa gac tac att tat gga ata 32478 Pro Thr Lys Pro Thr Lys Gly Arg Glu Asp Tyr Ile Tyr Gly Ile 6325 6330 6335 act tac tat caa gcc aca gat ggt aat ctc tat gag cta aaa act 32523 Thr Tyr Tyr Gln Ala Thr Asp Gly Asn Leu Tyr Glu Leu Lys Thr 6340 6345 6350 act att act cta aac cac agt gtc att agt tct cta tgt gca tat 32568 Thr Ile Thr Leu Asn His Ser Val Ile Ser Ser Leu Cys Ala Tyr 6355 6360 6365 gca atg cac att tca tgg tca tgg gac acc gta aca gag cca gag 32613 Ala Met His Ile Ser Trp Ser Trp Asp Thr Val Thr Glu Pro Glu 6370 6375 6380 aca aca ccc act act ctt att acc tcc ccc ttc tcc ttt tcc tat 32658 Thr Thr Pro Thr Thr Leu Ile Thr Ser Pro Phe Ser Phe Ser Tyr 6385 6390 6395 atc aga gaa gat gac tgacaacaaa aaataaagtt caaaattttt tattgaaaat 32713 Ile Arg Glu Asp Asp 6400 cagtttacag gattcgagta gttattttgc ctcccccttc ccatttcata gtatacacca 32773 atctctcccc ccgcacagct tggaacattt ggattccatt tgagatagtc atggatctag 32833 attctacatt ccacacagtt tcagagctag ctaatcttgg atcagtgata gatataaacc 32893 catcgggaaa gtccttcatg gtggtttcac agtccagttg ctgaggttgc ggctccggag 32953 tctggatcag agtcatctgg aagaagaacg atgggagtca taatccgaga acgggatcgg 33013 gcggttgtgt ctcatcaaac cccgaagcag tcgctgtctg cgccgctccg tgcgactgct 33073 gctgatggga tctgggtcca cagtctctcg aatcatgatt ttaatagccc tcagcattaa 33133 catcctggtg cgatgcgcac agcaccgcat tctaatctca cttaggtcac tgcagtaggt 33193 acagcacatc acaataatgt tgttcaacag gccataatta aaggcgctcc agccaaaact 33253 catctcaggg acaattgcta cagcgtggcc atcgtaccaa atcctgaggt aaatcaaatg 33313 gcgccccctc cagaacacac tgcccacata catgatctcc ttgggcatgt gcagattcac 33373 aatttctcgg taccatggac agcgctggtt tatcatgcag ccctgaataa ctttcctgaa 33433 ccaaatggcc agcactgctc ccccagcaat acattgaaga gaaccctgct gattacaatg 33493 acaatggaga acccacttct ctcgcccatg aaccacttgg gaataaaata tatctatatt 33553 ggcacagcac aagcatatat gcatacatct cctcatcacc cttaactctt caggggttaa 33613 aaccatatcc cagggaatag gaagctcttg caaaacagtg aaaccggcag aacaaggaag 33673 accacgaaca taacttacac tatgcatggt cagggtatta caatctggca acagcgggtg 33733 gtcctcggtc atagaagccc tggtctcatt ttcctcacag cgtggtaaag gggccctcat 33793 gcgaggatcc ctggtgtaag gttggtgcct ggcgcacgat gtcgagcgtg cacgcgacct 33853 cgttgtaatg gaggctcttc ctgacattct cgtattttgc agcgcaaaac ctggtcttag 33913 cacagcagac ttctcttcgc cttctatctc gtcgcctagc gcgttcagtg tggtaattga 33973 agtacagcca ttcccgcaga ttggtcaaaa gatcctcggc ctcagttgtc atgaaaactc 34033 catcatatct gatcgctcta ataaaatcat tcacggtaga caatgcaatt cccaaccaag 34093 caatgcaatt agcttgagtt tcgatcaggg gtgggggagg aagagatgga agaaccataa 34153 ttaattttta ttccaaacgg tcccgtagta cttcaaaatg cagatcgcgg agatggcacc 34213 tctcgccccc actgtgttga tgaaaaatga cagctaggtc aaacatgatg cgattttcaa 34273 ggtgctcaac ggtggcttca agcaaagcct ccactctcac atccaaaaac aaaagaatag 34333 caaaagcagg agcatgttct aattcctcaa tcatcatatt acattcctgt accattccca 34393 ggtaattttc atctttccag ccttgtatta ttcgtgttaa ttcttgttgt aaatccaatc 34453 cacacataag aaagagctcc cggagagcac cctccaccgg cattcttaaa cacaccctca 34513 tagtgaaaaa atatcgtgct cctctgtcac ctgcagcaaa ttgagaatgg caacatcaaa 34573 ctgaatgcca ttggctctaa gttcatctct aagttcaagt tgtaaatact ctttcatatc 34633 atcgccaaac tgcttggcca taggtccgcc aggaataaga gcgggggacg ctacagtgca 34693 gaacaagcga agacctcccc aattgcctcc agcaaaagtg aggttacaat aagcatactg 34753 agaacctcca gtgatatcat ccagtgtact ggaaagataa tcaggcagag cttctcgtat 34813 aaaattaata atagaaaagt tgtccagatg aacatttaaa gactgtggga tgcagatgca 34873 ataagttatc gcgctgcgtt ccagcattgt tagtatggtt agtctgtaaa aacaaaaaga 34933 gtaaaaaatt acatcacgct agcctggcga acgggtggat aaatcactct ctccaacacc 34993 aggcaggcta cagggtctcc agcgcgaccc tcgtaaaacc tgtccgtatg attaaaaagc 35053 aacaccgaaa gactttgctg atggccagca tggatgattt gtgaggaagc atacaatcca 35113 gaagtgttag tatcagttaa agaaaaaaat cggccaatat agcatctagg aacaattatg 35173 ctcaatctca aatgcagcaa agcgacacct cgtggatgca aagtaaaatc cacaggcgca 35233 taaaaataat acatattccc ctcttgcaca ggcagtgtag ctcccggccc ctccaaaaac 35293 acatacaaag cttcagcagc catagcttac cgcgcaagtc aggcagagca gacagataat 35353 gggatagctc taaactgtct gcccagcctg tgcgcaatat atagagaacc cttacactga 35413 cgtaattggg caaagtctaa aaaatcccgc caaaaaccag cacacgccca gaaactatgt 35473 cacccgctaa aaaaataatt ttcacttcct cgttccgtga gtgacgtcag ttcctctttc 35533 ccacgtgtcg tcactgccgg gtatcttgca acgtcaccac cccgcgccgg cccgccccct 35593 ttgaccgttg aacccgtagc caatcccctt ccgccctcca ttttcaaaag ctcatttgca 35653 tgttggcacc gttccattta taaggtatat tattgatgat g 35694 <210> 135 <211> 495 <212> PRT <213> Simian adenovirus 33 <400> 135 Met Asp Pro Pro Asn Pro Leu Gln Gln Gly Ile Arg Phe Gly Phe His 1 5 10 15 Ser Ser Ser Phe Val Glu Asn Met Glu Gly Ser Gln Asp Glu Asp Asn 20 25 30 Leu Arg Leu Leu Ala Ser Ala Ala Ser Gly Arg Ser Arg Asp Pro Glu 35 40 45 Thr Pro Thr Gly His Ala Ser Gly Ser Gly Gly Gly Ala Ala Gly Gly 50 55 60 Gln Ser Glu Ser Arg Pro Gly Pro Ser Gly Gly Gly Gly Gly Gly Val 65 70 75 80 Ala Asp Leu Phe Pro Glu Leu Arg Arg Val Leu Thr Arg Ser Thr Ser 85 90 95 Ser Gly Gln Asp Arg Gly Ile Lys Arg Glu Arg Asn Pro Ser Gly His 100 105 110 Asn Ser Arg Thr Glu Leu Ala Leu Ser Leu Met Ser Arg Arg Arg Pro 115 120 125 Glu Thr Val Trp Trp His Glu Val Gln Ser Glu Gly Arg Asp Glu Val 130 135 140 Ser Ile Leu Gln Glu Lys Tyr Ser Leu Glu Gln Leu Lys Thr Cys Trp 145 150 155 160 Leu Glu Pro Glu Asp Asp Trp Glu Val Ala Ile Gly Asn Tyr Ala Lys 165 170 175 Ile Ser Leu Arg Pro Asp Lys Gln Tyr Arg Ile Thr Lys Lys Ile Asn 180 185 190 Ile Arg Asn Ala Cys Tyr Ile Ser Gly Asn Gly Ala Glu Val Ile Ile 195 200 205 Asp Thr Gln Asp Lys Ala Ala Phe Arg Cys Cys Met Met Gly Met Trp 210 215 220 Pro Gly Val Val Gly Met Glu Ala Val Thr Leu Met Asn Ile Arg Phe 225 230 235 240 Arg Gly Asp Gly Tyr Asn Gly Ile Val Phe Met Ala Asn Thr Lys Leu 245 250 255 Ile Leu His Gly Cys Ser Phe Phe Gly Phe Asn Asn Thr Cys Val Glu 260 265 270 Ala Trp Gly Gln Val Ser Val Arg Gly Cys Ser Phe Tyr Ala Cys Trp 275 280 285 Ile Ala Thr Ser Gly Arg Val Lys Ser Gln Leu Ser Val Lys Lys Cys 290 295 300 Met Phe Glu Arg Cys Asn Leu Gly Ile Leu Asn Glu Gly Glu Ala Arg 305 310 315 320 Val Arg His Cys Ala Ala Thr Glu Thr Gly Cys Phe Ile Leu Ile Lys 325 330 335 Gly Asn Ala Ser Val Lys His Asn Met Ile Cys Gly Pro Ser Asp Glu 340 345 350 Arg Pro Tyr Gln Met Leu Thr Cys Ala Gly Gly His Cys Asn Met Leu 355 360 365 Ala Thr Val His Ile Val Ser His Ala Arg Lys Lys Trp Pro Val Phe 370 375 380 Glu His Asn Val Met Thr Lys Cys Thr Met His Ile Gly Gly Arg Arg 385 390 395 400 Gly Met Phe Met Pro Tyr Gln Cys Asn Met Asn His Val Lys Val Met 405 410 415 Leu Glu Pro Asp Ala Phe Ser Arg Met Ser Leu Thr Gly Ile Phe Asp 420 425 430 Met Asn Val Gln Leu Trp Lys Ile Leu Arg Tyr Asp Asp Thr Lys Ser 435 440 445 Arg Val Arg Ala Cys Glu Cys Gly Gly Lys His Ala Arg Phe Gln Pro 450 455 460 Val Cys Val Asp Val Thr Glu Asp Leu Arg Pro Asp His Leu Val Leu 465 470 475 480 Ala Cys Thr Gly Ala Glu Phe Gly Ser Ser Gly Glu Glu Thr Asp 485 490 495 <210> 136 <211> 138 <212> PRT <213> Simian adenovirus 33 <400> 136 Met Ser Gly Ser Ala Ser Phe Glu Gly Gly Val Phe Ser Pro Tyr Leu 1 5 10 15 Thr Gly Arg Leu Pro Pro Trp Ala Gly Val Arg Gln Asn Val Met Gly 20 25 30 Ser Thr Val Asp Gly Arg Pro Val Gln Pro Ala Asn Ser Ser Thr Leu 35 40 45 Thr Tyr Ala Thr Leu Ser Ser Ser Pro Leu Asp Ala Ala Ala Ala Ala 50 55 60 Ala Ala Ser Ala Ala Ala Asn Thr Val Leu Gly Met Gly Tyr Tyr Gly 65 70 75 80 Ser Ile Val Ala Asn Ser Ser Ser Ser Asn Asn Pro Ser Thr Leu Ala 85 90 95 Glu Asp Lys Leu Leu Val Leu Leu Ala Gln Leu Glu Ala Leu Thr Gln 100 105 110 Arg Leu Gly Glu Leu Ser Gln Gln Val Ala Gln Leu Arg Glu Gln Thr 115 120 125 Glu Ser Ala Val Ala Thr Ala Lys Ser Lys 130 135 <210> 137 <211> 390 <212> PRT <213> Simian adenovirus 33 <400> 137 Met His Pro Val Leu Arg Gln Met Arg Pro Gln Gln Gln Ala Pro Ser 1 5 10 15 Gln Gln Gln Gln Leu Gln Gln Gln Pro Gln Lys Ala Leu Pro Ala Pro 20 25 30 Ala Thr Thr Ala Ala Ala Ala Val Ser Gly Ala Gly Gln Pro Ala Tyr 35 40 45 Asp Leu Asp Leu Glu Glu Gly Glu Gly Leu Ala Arg Leu Gly Ala Pro 50 55 60 Ser Pro Glu Arg His Pro Arg Val Gln Leu Lys Lys Asp Ser Arg Glu 65 70 75 80 Ala Tyr Val Pro His Gln Asn Leu Phe Arg Asp Arg Ser Gly Glu Glu 85 90 95 Pro Glu Glu Met Arg Ala Ser Arg Phe Asn Ala Gly Arg Glu Leu Arg 100 105 110 His Gly Leu Asp Arg Arg Arg Val Leu Arg Asp Glu Asp Phe Glu Val 115 120 125 Asp Glu Ala Thr Gly Ile Ser Ser Ala Arg Ala His Val Ala Ala Ala 130 135 140 Asn Leu Val Ser Ala Tyr Glu Gln Thr Val Lys Glu Glu Arg Asn Phe 145 150 155 160 Gln Lys Ser Phe Asn Asn His Val Arg Thr Leu Ile Ala Arg Glu Glu 165 170 175 Val Thr Leu Gly Leu Met His Leu Trp Asp Leu Met Glu Ala Ile Thr 180 185 190 Gln Asn Pro Thr Ser Lys Pro Leu Thr Ala Gln Leu Phe Leu Val Val 195 200 205 Gln His Ser Arg Asp Asn Glu Ala Phe Arg Glu Ala Leu Leu Asn Ile 210 215 220 Thr Glu Pro Glu Gly Arg Trp Leu Tyr Asp Leu Ile Asn Ile Leu Gln 225 230 235 240 Ser Ile Val Val Gln Glu Arg Ser Leu Gly Leu Ala Glu Lys Val Ala 245 250 255 Ala Ile Asn Tyr Ser Val Leu Ser Leu Gly Lys Tyr Tyr Ala Arg Lys 260 265 270 Ile Tyr Lys Thr Pro Tyr Val Pro Ile Asp Lys Glu Val Lys Ile Asp 275 280 285 Gly Phe Tyr Met Arg Met Thr Leu Lys Val Leu Thr Leu Ser Asp Asp 290 295 300 Leu Gly Val Tyr Arg Asn Asp Arg Met His Arg Ala Val Ser Ala Ser 305 310 315 320 Arg Arg Arg Glu Leu Ser Asp Arg Glu Leu Met His Ser Leu Gln Arg 325 330 335 Ala Leu Thr Gly Ala Gly Thr Glu Gly Glu Asn Tyr Phe Asp Met Gly 340 345 350 Ala Asp Leu Gln Trp Gln Pro Ser Arg Arg Ala Leu Glu Ala Ala Gly 355 360 365 Cys Glu Leu Pro Tyr Ile Glu Glu Val Asp Glu Gly Glu Asp Glu Glu 370 375 380 Gly Glu Tyr Leu Glu Asp 385 390 <210> 138 <211> 588 <212> PRT <213> Simian adenovirus 33 <400> 138 Met Glu Gln Gln Gln Ala Pro Asp Pro Ala Met Arg Ala Ala Leu Gln 1 5 10 15 Ser Gln Pro Ser Gly Ile Asn Ser Ser Asp Asp Trp Thr Gln Ala Met 20 25 30 Gln Arg Ile Met Ala Leu Thr Thr Arg Asn Pro Glu Ala Phe Arg Gln 35 40 45 Gln Pro Gln Ala Asn Arg Leu Ser Ala Ile Leu Glu Ala Val Val Pro 50 55 60 Ser Arg Ser Asn Pro Thr His Glu Lys Val Leu Ala Ile Val Asn Ala 65 70 75 80 Leu Val Glu Asn Lys Ala Ile Arg Pro Asp Glu Ala Gly Leu Val Tyr 85 90 95 Asn Ala Leu Leu Glu Arg Val Ala Arg Tyr Asn Ser Ser Asn Val Gln 100 105 110 Thr Asn Leu Asp Arg Met Val Ser Asp Val Arg Glu Ala Val Ser Gln 115 120 125 Arg Glu Arg Phe Gln Arg Asp Ala Asn Leu Gly Ser Leu Val Ala Leu 130 135 140 Asn Ala Phe Leu Ser Thr Gln Pro Ala Asn Val Pro Arg Gly Gln Gln 145 150 155 160 Asp Tyr Thr Asn Phe Leu Ser Ala Leu Arg Leu Met Val Ala Glu Val 165 170 175 Pro Gln Ser Glu Val Tyr Gln Ser Gly Pro Asp Tyr Phe Phe Gln Thr 180 185 190 Ser Arg Gln Gly Leu Gln Thr Val Asn Leu Thr Gln Ala Phe Lys Asn 195 200 205 Leu Lys Gly Leu Trp Gly Val His Ala Pro Val Gly Asp Arg Ala Thr 210 215 220 Val Ser Ser Leu Leu Thr Pro Asn Ser Arg Leu Leu Leu Leu Leu Val 225 230 235 240 Ser Pro Phe Thr Asp Ser Gly Ser Ile Asp Arg Asn Ser Tyr Leu Gly 245 250 255 Tyr Leu Leu Asn Leu Tyr Arg Glu Ala Ile Gly Gln Ser Gln Val Asp 260 265 270 Glu Gln Thr Tyr Gln Glu Ile Thr Gln Val Ser Arg Ala Leu Gly Gln 275 280 285 Glu Asp Thr Gly Ser Leu Glu Ala Thr Leu Asn Phe Leu Leu Thr Asn 290 295 300 Arg Ser Gln Lys Ile Pro Pro Gln Tyr Ala Leu Thr Ala Glu Glu Glu 305 310 315 320 Arg Ile Leu Arg Tyr Val Gln Gln Ser Val Gly Leu Phe Leu Met Gln 325 330 335 Glu Gly Ala Thr Pro Thr Ala Ala Leu Asp Met Thr Ala Arg Asn Met 340 345 350 Glu Pro Ser Met Tyr Ala Ser Asn Arg Pro Phe Ile Asn Lys Leu Leu 355 360 365 Asp Tyr Leu His Arg Ala Ala Ala Met Asn Ser Asp Tyr Phe Thr Asn 370 375 380 Ala Ile Leu Asn Pro His Trp Leu Pro Pro Pro Gly Phe Tyr Thr Gly 385 390 395 400 Glu Tyr Asp Met Pro Asp Pro Asn Asp Gly Phe Leu Trp Asp Asp Val 405 410 415 Asp Ser Ser Ile Phe Ser Pro Pro Pro Gly Tyr Thr Val Trp Lys Lys 420 425 430 Glu Gly Gly Asp Arg Arg His Ser Ser Val Ser Leu Ser Gly Thr Ala 435 440 445 Gly Ala Ala Ala Ala Val Pro Glu Ala Ala Ser Pro Phe Pro Ser Leu 450 455 460 Pro Phe Ser Leu Asn Ser Val Arg Ser Ser Glu Leu Gly Arg Ile Thr 465 470 475 480 Arg Pro Arg Leu Met Gly Glu Asp Glu Tyr Leu Asn Asp Ser Leu Leu 485 490 495 Arg Pro Glu Arg Glu Lys Asn Phe Pro Asn Asn Gly Ile Glu Ser Leu 500 505 510 Val Asp Lys Met Ser Arg Trp Lys Thr Tyr Ala Gln Asp His Arg Asp 515 520 525 Glu Pro Arg Ile Leu Gly Ala Thr Ser Gly Ala Thr Arg Arg Arg Gln 530 535 540 Arg His Asp Arg Gln Arg Gly Leu Val Trp Asp Asp Glu Asp Ser Ala 545 550 555 560 Asp Asp Ser Ser Val Leu Asp Leu Gly Gly Arg Gly Val Gly Asn Pro 565 570 575 Phe Ala His Leu Arg Pro Arg Phe Gly Arg Met Leu 580 585 <210> 139 <211> 571 <212> PRT <213> Simian adenovirus 33 <400> 139 Met Met Arg Arg Arg Ala Val Leu Gly Gly Ala Val Val Tyr Pro Glu 1 5 10 15 Gly Pro Pro Pro Ser Tyr Glu Ser Val Met Gln Gln Gln Ala Ala Ala 20 25 30 Ala Met Met Gln Pro Pro Leu Glu Ala Pro Phe Val Pro Pro Arg Tyr 35 40 45 Leu Ala Pro Thr Glu Gly Arg Asn Ser Ile Arg Tyr Ser Glu Leu Ser 50 55 60 Pro Gln Tyr Asp Thr Thr Lys Leu Tyr Leu Val Asp Asn Lys Ser Ala 65 70 75 80 Asp Ile Ala Ser Leu Asn Tyr Gln Asn Asp His Ser Asn Phe Leu Thr 85 90 95 Thr Val Val Gln Asn Asn Asp Phe Thr Pro Thr Glu Ala Ser Thr Gln 100 105 110 Thr Ile Asn Phe Asp Glu Arg Ser Arg Trp Gly Gly Gln Leu Lys Thr 115 120 125 Ile Met His Thr Asn Met Pro Asn Val Asn Glu Tyr Met Phe Ser Asn 130 135 140 Lys Phe Lys Ala Arg Val Met Val Ser Arg Lys Ala Pro Glu Gly Val 145 150 155 160 Thr Val Asp Asp Lys Tyr Asp His Lys Gln Asp Ile Leu Lys Tyr Glu 165 170 175 Trp Phe Glu Phe Thr Leu Pro Glu Gly Asn Phe Ser Ala Thr Met Thr 180 185 190 Ile Asp Leu Met Asn Asn Ala Ile Ile Asp Asn Tyr Leu Gly Val Gly 195 200 205 Arg Gln Asn Gly Val Leu Glu Ser Asp Ile Gly Val Lys Phe Asp Thr 210 215 220 Arg Asn Phe Arg Leu Gly Trp Asp Pro Glu Thr Lys Leu Ile Met Pro 225 230 235 240 Gly Val Tyr Thr Tyr Glu Ala Phe His Pro Asp Ile Val Leu Leu Pro 245 250 255 Gly Cys Gly Val Asp Phe Thr Glu Ser Arg Leu Ser Asn Leu Leu Gly 260 265 270 Ile Arg Lys Arg His Pro Phe Gln Glu Gly Phe Lys Ile Met Tyr Glu 275 280 285 Asp Leu Glu Gly Gly Asn Ile Pro Ala Leu Leu Asp Val Thr Ala Tyr 290 295 300 Glu Glu Ser Lys Lys Asp Thr Thr Thr Glu Thr Gly Glu Lys Ala Val 305 310 315 320 Val Glu Ser Glu Thr Glu Ala Met Thr Glu Thr Thr Thr Leu Ala Val 325 330 335 Ala Glu Glu Thr Ser Glu Asp Asp Asn Ile Thr Arg Gly Asp Thr Tyr 340 345 350 Ile Thr Glu Lys Gln Lys Arg Glu Ala Ala Ala Ala Glu Ala Glu Leu 355 360 365 Leu Leu Met Ala Glu Val Lys Lys Glu Leu Lys Ile Gln Pro Leu Glu 370 375 380 Lys Asp Ser Lys Ser Arg Ser Tyr Asn Val Leu Glu Asp Lys Ile Asn 385 390 395 400 Thr Ala Tyr Arg Ser Trp Tyr Leu Ser Tyr Asn Tyr Gly Asp Pro Glu 405 410 415 Lys Gly Ile Arg Ser Trp Thr Leu Leu Thr Thr Ser Asp Val Thr Cys 420 425 430 Gly Ala Glu Gln Val Tyr Trp Ser Leu Pro Asp Met Met Gln Asp Pro 435 440 445 Val Thr Phe Arg Ser Thr Arg Gln Val Asn Asn Tyr Pro Val Val Gly 450 455 460 Ala Glu Leu Met Pro Val Phe Ser Lys Ser Phe Tyr Asn Glu Gln Ala 465 470 475 480 Val Tyr Ser Gln Gln Leu Arg Gln Ser Thr Ser Leu Thr His Val Phe 485 490 495 Asn Arg Phe Pro Glu Asn Gln Ile Leu Ile Arg Pro Pro Ala Pro Thr 500 505 510 Ile Thr Thr Val Ser Glu Asn Val Pro Ala Leu Thr Asp His Gly Thr 515 520 525 Leu Pro Leu Arg Ser Ser Ile Arg Gly Val Gln Arg Val Thr Val Thr 530 535 540 Asp Ala Arg Arg Arg Thr Cys Pro Tyr Val Tyr Lys Ala Leu Gly Ile 545 550 555 560 Val Ala Pro Arg Val Leu Ser Ser Arg Thr Phe 565 570 <210> 140 <211> 191 <212> PRT <213> Simian adenovirus 33 <400> 140 Met Ser Ile Leu Ile Ser Pro Ser Asn Asn Thr Gly Trp Gly Leu Arg 1 5 10 15 Ala Pro Thr Lys Met Tyr Gly Gly Ala Arg Lys Arg Ser Thr Gln His 20 25 30 Pro Val Arg Val Arg Gly His Phe Arg Ala Pro Trp Gly Ala Leu Lys 35 40 45 Gly Arg Thr Arg Thr Arg Thr Thr Val Asp Asp Val Ile Asp Gln Val 50 55 60 Val Ala Asp Ala Arg Asn Tyr Thr Pro Thr Ala Pro Thr Ser Thr Val 65 70 75 80 Asp Ala Val Ile Asp Ser Val Val Ala Asn Ala Arg Ala Tyr Ala Arg 85 90 95 Arg Lys Ser Arg Arg Arg Arg Ile Ala Arg Arg His Arg Ala Thr Pro 100 105 110 Ala Met Arg Ala Ala Arg Ala Leu Leu Arg Arg Ala Lys Arg Val Gly 115 120 125 Arg Arg Ala Met Leu Arg Ala Ala Arg Arg Ala Ala Ser Gly Ala Ser 130 135 140 Ser Gly Arg Ser Arg Arg Arg Ala Ala Thr Ala Ala Ala Ala Ala Ile 145 150 155 160 Ala Asn Met Ala Gln Pro Arg Arg Gly Asn Val Tyr Trp Val Arg Asp 165 170 175 Ala Ser Gly Gln Arg Val Pro Val Arg Thr Arg Pro Pro Arg Thr 180 185 190 <210> 141 <211> 350 <212> PRT <213> Simian adenovirus 33 <400> 141 Met Ser Lys Arg Lys Tyr Lys Glu Glu Met Leu Gln Val Ile Ala Pro 1 5 10 15 Glu Ile Tyr Gly Pro Pro Val Lys Asp Glu Lys Lys Pro Arg Lys Ile 20 25 30 Lys Arg Val Lys Lys Asp Lys Lys Glu Glu Asp Gly Asp Asp Gly Leu 35 40 45 Val Glu Phe Val Arg Glu Phe Ala Pro Arg Arg Arg Val Gln Trp Arg 50 55 60 Gly Arg Lys Val Arg Gln Val Leu Arg Pro Gly Thr Thr Val Val Phe 65 70 75 80 Thr Pro Gly Glu Arg Ser Ser Ser Thr Phe Lys Arg Ser Tyr Asp Glu 85 90 95 Val Tyr Gly Asp Asp Asp Ile Leu Glu Gln Ala Ala Asp Arg Leu Gly 100 105 110 Glu Phe Ala Tyr Gly Lys Arg Thr Arg Ser Ser Pro Lys Glu Glu Ala 115 120 125 Val Ser Ile Pro Leu Asp His Gly Asn Pro Thr Pro Ser Leu Lys Pro 130 135 140 Val Thr Leu Gln Gln Val Leu Pro Val Pro Pro Arg Arg Gly Val Lys 145 150 155 160 Arg Glu Gly Glu Asp Leu Tyr Pro Thr Met Gln Leu Met Val Pro Lys 165 170 175 Arg Gln Arg Leu Glu Asp Val Leu Glu Lys Met Lys Val Asp Pro Asp 180 185 190 Ile Gln Pro Glu Val Lys Val Arg Pro Ile Lys Glu Val Ala Pro Gly 195 200 205 Leu Gly Val Gln Thr Val Asp Ile Lys Ile Pro Thr Glu Ser Met Glu 210 215 220 Val Gln Thr Glu Pro Ala Lys Pro Thr Ala Thr Ser Ile Glu Val Gln 225 230 235 240 Thr Asp Pro Trp Met Pro Ala Pro Val Ala Ala His Ser Thr Thr Arg 245 250 255 Arg Pro Arg Arg Lys Tyr Gly Pro Ala Ser Leu Leu Met Pro Asn Tyr 260 265 270 Ala Leu His Pro Ser Ile Ile Pro Thr Pro Gly Tyr Arg Gly Thr Arg 275 280 285 Tyr Tyr Arg Ser Arg Ser Ser Thr Ser Arg Arg Arg Lys Thr Pro Ala 290 295 300 Ser Arg Thr Arg Arg Arg Arg Arg Arg Thr Thr Ala Ser Lys Leu Thr 305 310 315 320 Pro Ala Ala Leu Val Arg Arg Val Tyr Arg Asp Gly Arg Ala Glu Pro 325 330 335 Leu Thr Leu Pro Arg Ala Arg Tyr His Pro Ser Ile Thr Thr 340 345 350 <210> 142 <211> 75 <212> PRT <213> Simian adenovirus 33 <400> 142 Met Ala Leu Thr Cys Arg Leu Arg Val Pro Ile Thr Gly Tyr Arg Gly 1 5 10 15 Arg Asn Ser Arg Arg Arg Arg Met Leu Gly Arg Gly Met Arg Arg His 20 25 30 Arg Arg Arg Arg Ala Ile Ser Lys Arg Leu Gly Gly Gly Phe Leu Pro 35 40 45 Ala Leu Ile Pro Ile Ile Ala Ala Ala Ile Gly Ala Ile Pro Gly Ile 50 55 60 Ala Ser Val Ala Val Gln Ala Ser Gln Arg His 65 70 75 <210> 143 <211> 250 <212> PRT <213> Simian adenovirus 33 <400> 143 Met Glu Asp Ile Asn Phe Ser Ser Leu Ala Pro Arg His Gly Thr Arg 1 5 10 15 Pro Tyr Met Gly Thr Trp Ser Asp Ile Gly Thr Ser Gln Leu Asn Gly 20 25 30 Gly Ala Phe Asn Trp Ser Ser Ile Trp Ser Gly Leu Lys Asn Phe Gly 35 40 45 Ser Thr Ile Lys Thr Tyr Gly Asn Lys Ala Trp Asn Ser Ser Thr Gly 50 55 60 Gln Ala Leu Arg Asn Lys Leu Lys Glu Gln Asn Phe Gln Gln Lys Val 65 70 75 80 Val Asp Gly Ile Ala Ser Gly Ile Asn Gly Val Val Asp Leu Ala Asn 85 90 95 Gln Ala Val Gln Lys Gln Ile Asn Ser Arg Leu Asp Pro Pro Pro Ala 100 105 110 Ala Pro Gly Glu Met Glu Val Glu Glu Glu Leu Pro Pro Leu Glu Lys 115 120 125 Arg Gly Asp Lys Arg Pro Arg Pro Asp Leu Glu Glu Thr Leu Val Thr 130 135 140 Arg Ala Asp Glu Pro Pro Ser Tyr Glu Glu Ala Val Lys Leu Gly Met 145 150 155 160 Pro Thr Thr Lys Pro Ile Ala Pro Met Ala Thr Gly Val Met Lys Pro 165 170 175 Ser Gln Ser His Arg Pro Ala Thr Leu Asp Leu Pro Pro Pro Pro Ala 180 185 190 Ala Ala Ala Pro Val Pro Lys Pro Val Ala Thr Arg Lys Pro Thr Ala 195 200 205 Ala Gln Pro Val Ala Val Ala Arg Pro Arg Pro Gly Gly Thr Pro Arg 210 215 220 Pro Asn Ala Asn Trp Gln Ser Thr Leu Asn Ser Ile Val Gly Leu Gly 225 230 235 240 Val Gln Ser Val Lys Arg Arg Arg Cys Phe 245 250 <210> 144 <211> 951 <212> PRT <213> Simian adenovirus 33 <400> 144 Met Ala Thr Pro Ser Met Leu Pro Gln Trp Ala Tyr Met His Ile Ala 1 5 10 15 Gly Gln Asp Ala Ser Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala 20 25 30 Arg Ala Thr Asp Thr Tyr Phe Asn Leu Gly Asn Lys Phe Arg Asn Pro 35 40 45 Thr Val Ala Pro Thr His Asp Val Thr Thr Asp Arg Ser Gln Arg Leu 50 55 60 Met Leu Arg Phe Val Pro Val Asp Arg Glu Asp Asn Thr Tyr Ser Tyr 65 70 75 80 Lys Val Arg Tyr Thr Leu Ala Val Gly Asp Asn Arg Val Leu Asp Met 85 90 95 Ala Ser Thr Phe Phe Asp Ile Arg Gly Val Leu Asp Arg Gly Pro Ser 100 105 110 Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser Leu Ala Pro Lys Gly 115 120 125 Ala Pro Asn Thr Cys Gln Trp Ile Ala Lys Gly Ala Pro Val Thr Asp 130 135 140 Gln Asp Asn Glu Glu Gln Glu Leu Thr Asp Val Thr Tyr Ala Phe Gly 145 150 155 160 Asn Ala Pro Val Gln Ala Glu Ala Lys Ile Thr Lys Asp Gly Leu Pro 165 170 175 Val Gly Leu Glu Ile Thr Glu Asp Glu Gln Lys Ser Ile Tyr Ala Asp 180 185 190 Lys Leu Tyr Gln Pro Glu Pro Gln Ile Gly Asp Glu Gln Trp His Asp 195 200 205 Thr Thr Gly Thr Asn Glu Gln Tyr Gly Gly Arg Ala Leu Lys Pro Ala 210 215 220 Thr Asn Met Lys Pro Cys Tyr Gly Ser Phe Ala Arg Pro Thr Asn Lys 225 230 235 240 Lys Gly Gly Gln Ala Lys Thr Arg Lys Ile Glu Lys Glu Glu Asn Gly 245 250 255 Val Lys Thr Val Thr Glu Glu Ala Asp Ile Asp Met Asp Phe Tyr Asp 260 265 270 Leu Arg Ser Gln Arg Ala Asn Phe Asp Pro Lys Ile Val Leu Tyr Ser 275 280 285 Glu Asn Val Asn Leu Glu Thr Pro Asp Thr His Ile Val Tyr Lys Pro 290 295 300 Gly Thr Asp Glu Thr Ser Ser Ser Val Asn Leu Gly Gln Gln Ala Met 305 310 315 320 Pro Asn Arg Pro Asn Tyr Ile Gly Phe Arg Asp Asn Phe Ile Gly Leu 325 330 335 Met Phe Tyr Asn Ser Thr Gly Asn Met Gly Val Leu Ala Gly Gln Ala 340 345 350 Ser Gln Leu Asn Ala Val Val Asp Leu Gln Asp Arg Asn Thr Glu Leu 355 360 365 Ser Tyr Gln Leu Leu Leu Asp Ser Leu Gly Asp Arg Thr Arg Tyr Phe 370 375 380 Ser Met Trp Asn Gln Ala Val Asp Ser Tyr Asp Pro Asp Val Arg Ile 385 390 395 400 Ile Glu Asn His Gly Val Glu Asp Glu Leu Pro Asn Tyr Cys Phe Pro 405 410 415 Leu Asp Gly Val Gly Pro Ile Thr Gly Thr Tyr Gln Gly Val Glu Pro 420 425 430 Asp Gly Asn Asn Gly Asn Trp Lys Lys Asn Thr Asn Ile Asn Gly Ala 435 440 445 Asn Glu Ile Gly Lys Gly Asn Asn Tyr Ala Met Glu Ile Asn Leu Gln 450 455 460 Ala Asn Leu Trp Arg Ser Phe Leu Tyr Ser Asn Val Ala Leu Tyr Leu 465 470 475 480 Pro Asp Gly Tyr Lys Tyr Thr Pro Ala Asn Val Thr Leu Pro Glu Asn 485 490 495 Lys Asn Thr Tyr Gly Tyr Ile Asn Gly Arg Val Val Ser Pro Ser Leu 500 505 510 Val Asp Ser Tyr Ile Asn Ile Gly Ala Arg Trp Ser Leu Asp Leu Met 515 520 525 Asp Asn Val Asn Pro Phe Asn His His Arg Asn Ala Gly Leu Arg Tyr 530 535 540 Arg Ser Met Leu Leu Gly Asn Gly Arg Tyr Val Pro Phe His Ile Gln 545 550 555 560 Val Pro Gln Lys Phe Phe Ala Val Lys Asn Leu Leu Leu Leu Pro Gly 565 570 575 Ser Tyr Thr Tyr Glu Trp Asn Phe Arg Lys Asp Val Asn Met Val Leu 580 585 590 Gln Ser Ser Leu Gly Asn Asp Leu Arg Thr Asp Gly Ala Ser Ile Ser 595 600 605 Phe Thr Ser Ile Asn Leu Tyr Ala Thr Phe Phe Pro Met Ala His Asn 610 615 620 Thr Ala Ser Thr Leu Glu Ala Met Leu Arg Asn Asp Thr Asn Asp Gln 625 630 635 640 Ser Phe Asn Asp Tyr Leu Ser Ala Ala Asn Met Leu Tyr Pro Ile Pro 645 650 655 Ala Asn Ala Thr Asn Ile Pro Ile Ser Ile Pro Ser Arg Asn Trp Ala 660 665 670 Ala Phe Arg Gly Trp Ser Phe Thr Arg Leu Lys Thr Lys Glu Thr Pro 675 680 685 Ser Leu Gly Ser Gly Phe Asp Pro Tyr Phe Val Tyr Ser Gly Ser Ile 690 695 700 Pro Tyr Leu Asp Gly Thr Phe Tyr Leu Asn His Thr Phe Lys Lys Val 705 710 715 720 Ser Ile Met Phe Asp Ser Ser Val Ser Trp Pro Gly Asn Asp Arg Leu 725 730 735 Leu Thr Pro Asn Glu Phe Glu Ile Lys Arg Thr Val Asp Gly Glu Gly 740 745 750 Tyr Asn Val Ala Gln Cys Asn Met Thr Lys Asp Trp Phe Leu Val Gln 755 760 765 Met Leu Ala Asn Tyr Asn Ile Gly Tyr Gln Gly Phe Tyr Ile Pro Glu 770 775 780 Gly Tyr Lys Asp Arg Met Tyr Ser Phe Phe Arg Asn Phe Gln Pro Met 785 790 795 800 Ser Arg Gln Val Val Asp Glu Ile Asn Tyr Lys Glu Tyr Gln Ala Val 805 810 815 Thr Leu Ala Tyr Gln His Asn Asn Ser Gly Phe Val Gly Tyr His Ala 820 825 830 Pro Thr Leu Arg Gln Gly Gln Pro Tyr Pro Ala Asn Tyr Pro Tyr Pro 835 840 845 Leu Ile Gly Thr Thr Ala Val Thr Ser Val Thr Gln Lys Lys Phe Leu 850 855 860 Cys Asp Arg Thr Met Trp Arg Ile Pro Phe Ser Ser Asn Phe Met Ser 865 870 875 880 Met Gly Ala Leu Thr Asp Leu Gly Gln Asn Met Leu Tyr Ala Asn Ser 885 890 895 Ala His Ala Leu Asp Met Thr Phe Glu Val Asp Pro Met Asp Glu Pro 900 905 910 Thr Leu Leu Tyr Leu Leu Phe Glu Val Phe Asp Val Val Arg Val His 915 920 925 Gln Pro His Arg Gly Val Ile Glu Ala Val Tyr Leu Arg Thr Pro Phe 930 935 940 Ser Ala Gly Asn Ala Thr Thr 945 950 <210> 145 <211> 209 <212> PRT <213> Simian adenovirus 33 <400> 145 Met Thr Cys Gly Ser Gly Asn Gly Ser Ser Glu Gln Glu Leu Lys Ala 1 5 10 15 Ile Val Arg Asp Leu Gly Cys Gly Pro Tyr Phe Leu Gly Thr Phe Asp 20 25 30 Lys Arg Phe Pro Gly Phe Met Ala Pro Asp Lys Leu Ala Cys Ala Ile 35 40 45 Val Asn Thr Ala Gly Arg Glu Thr Gly Gly Glu His Trp Leu Ala Phe 50 55 60 Gly Trp Asn Pro Arg Ser Asn Thr Cys Tyr Leu Phe Asp Pro Phe Gly 65 70 75 80 Phe Ser Asp Glu Arg Leu Lys Gln Ile Tyr Gln Phe Glu Tyr Glu Gly 85 90 95 Leu Leu Arg Arg Ser Ala Leu Ala Thr Lys Asp Arg Cys Ile Thr Leu 100 105 110 Glu Lys Ser Thr Gln Thr Val Gln Gly Pro Arg Ser Ala Ala Cys Gly 115 120 125 Leu Phe Cys Cys Met Phe Leu His Ala Phe Val His Trp Pro Asp Arg 130 135 140 Pro Met Asp Gly Asn Pro Thr Met Lys Leu Leu Thr Gly Val Pro Asn 145 150 155 160 Ser Met Leu Gln Ser Pro Gln Val Gln Pro Thr Leu Arg Arg Asn Gln 165 170 175 Glu Ala Leu Tyr Arg Phe Leu Asn Thr His Ser Ser Tyr Phe Arg Ser 180 185 190 His Arg Ala Arg Ile Glu Arg Ala Thr Ala Phe Asp Arg Met Asp Met 195 200 205 Gln <210> 146 <211> 833 <212> PRT <213> Simian adenovirus 33 <400> 146 Met Glu Thr Gln Pro Ser Leu Pro Thr Ser Leu Gln Ala Pro Ser His 1 5 10 15 Leu Ala Pro Ser Ser Asp Glu Glu Glu Ser Leu Thr Thr Pro Pro Pro 20 25 30 Ser Pro Ala Thr Thr Thr Ser Thr Leu Glu Asp Glu Glu Glu Val Asp 35 40 45 Ala Pro Gln Glu Met Gln Ala Gln Asp Met Glu Asp Val Lys Ala Glu 50 55 60 Glu Ile Glu Ala Asp Val Glu Gln Asp Pro Gly Tyr Val Thr Pro Ala 65 70 75 80 Glu His Glu Glu Glu Leu Lys Arg Phe Leu Asp Arg Glu Glu Val Asp 85 90 95 Ser Arg Pro Glu His Gln Ala Asp Gly Asp His Gln Glu Ala Gly Leu 100 105 110 Gly Asp His Val Ala Asp Tyr Leu Thr Gly Leu Gly Ser Glu Asp Val 115 120 125 Leu Leu Lys His Leu Ala Arg Gln Ser Ile Ile Val Lys Asp Ala Val 130 135 140 Leu Asp Arg Thr Glu Val Pro Ile Ser Val Glu Glu Leu Ser Arg Ala 145 150 155 160 Tyr Glu Leu Asn Leu Phe Ser Pro Arg Val Pro Pro Lys Arg Gln Pro 165 170 175 Asn Gly Thr Cys Glu Pro Asn Pro Arg Leu Asn Phe Tyr Pro Ala Phe 180 185 190 Thr Val Pro Glu Val Leu Ala Thr Tyr His Ile Phe Phe Lys Asn Gln 195 200 205 Lys Ile Pro Val Ser Cys Arg Ala Asn Arg Thr Arg Ala Asp Ala Leu 210 215 220 Leu Asn Leu Gly Pro Gly Ala Cys Leu Pro Asp Ile Ala Ser Leu Glu 225 230 235 240 Glu Val Pro Lys Ile Phe Glu Gly Leu Gly Ser Asp Glu Thr Arg Ala 245 250 255 Ala Asn Ala Leu Gln Gln Gly Glu Asn Gly Met Asp Glu His His Ser 260 265 270 Ala Leu Val Glu Leu Glu Gly Asp Asn Ala Arg Leu Ala Val Leu Lys 275 280 285 Arg Ser Ile Val Val Thr His Phe Ala Tyr Pro Ala Val Asn Leu Pro 290 295 300 Pro Lys Val Met Ser Ala Val Met Asp His Leu Leu Ile Lys Arg Ala 305 310 315 320 Ser Pro Leu Ser Glu Asp Gln Asn Met Gln Asp Pro Asp Ala Ser Asp 325 330 335 Glu Gly Lys Pro Val Val Ser Asp Glu Gln Leu Ser Arg Trp Leu Gly 340 345 350 Thr Asn Ser Pro Arg Asp Leu Glu Glu Arg Arg Lys Leu Met Met Ala 355 360 365 Val Val Leu Val Thr Val Glu Leu Glu Cys Leu Arg Arg Phe Phe Thr 370 375 380 Asp Pro Glu Thr Leu Arg Lys Leu Glu Glu Asn Leu His Tyr Thr Phe 385 390 395 400 Arg His Gly Phe Val Arg Gln Ala Cys Lys Ile Ser Asn Val Glu Leu 405 410 415 Thr Asn Leu Val Ser Tyr Met Gly Ile Leu His Glu Asn Arg Leu Gly 420 425 430 Gln Ser Val Leu His Thr Thr Leu Lys Gly Glu Ala Arg Arg Asp Tyr 435 440 445 Ile Arg Asp Cys Val Tyr Leu Tyr Leu Cys His Thr Trp Gln Thr Gly 450 455 460 Met Gly Val Trp Gln Gln Cys Leu Glu Glu Gln Asn Leu Lys Glu Leu 465 470 475 480 Asp Lys Leu Leu Gln Arg Ser Leu Lys Ala Leu Trp Thr Gly Phe Asp 485 490 495 Glu Arg Thr Val Ala Ser Asp Leu Ala Asp Ile Ile Phe Pro Glu Arg 500 505 510 Leu Arg Val Thr Leu Arg Asn Gly Leu Pro Asp Phe Met Ser Gln Ser 515 520 525 Met Leu Asn Asn Phe Arg Ser Phe Ile Leu Glu Arg Ser Gly Ile Leu 530 535 540 Pro Ala Thr Cys Cys Ala Leu Pro Ser Asp Phe Val Pro Leu Ser Tyr 545 550 555 560 Arg Glu Cys Pro Pro Pro Leu Trp Ser His Cys Tyr Leu Phe Arg Leu 565 570 575 Ala Asn Tyr Leu Ser Tyr His Ser Asp Val Ile Glu Asp Val Ser Gly 580 585 590 Asp Gly Leu Leu Glu Cys His Cys Arg Cys Asn Leu Cys Thr Pro His 595 600 605 Arg Ser Leu Ala Cys Asn Pro Gln Leu Leu Ser Glu Thr Gln Ile Ile 610 615 620 Gly Thr Phe Glu Leu Gln Gly Pro Ser Ser Glu Gly Glu Gly Ser Ser 625 630 635 640 Pro Gly Gln Ser Leu Lys Leu Thr Pro Gly Leu Trp Thr Ser Ala Tyr 645 650 655 Leu Arg Lys Phe His Pro Glu Asp Tyr His Pro Tyr Glu Ile Arg Phe 660 665 670 Tyr Glu Asp Gln Ser His Pro Pro Lys Val Glu Leu Ser Ala Cys Val 675 680 685 Ile Thr Gln Gly Ala Ile Leu Ala Gln Leu Gln Ala Ile Gln Lys Ser 690 695 700 Arg Gln Glu Phe Leu Met Lys Lys Gly Ser Gly Val Tyr Leu Asp Pro 705 710 715 720 Gln Thr Gly Glu Glu Leu Asn Thr Arg Phe Pro Gln Asp Val Pro Ala 725 730 735 Pro Arg Lys Gln Glu Ala Glu Gly Ala Ala Ala Ala Pro Arg Gly Tyr 740 745 750 Gly Gly Arg Leu Gly Gln Ser Gly Arg Gly Ser Gly Gly Asp Gly Arg 755 760 765 Leu Gly Gln Pro Gly Arg Gly Gly Gly Gln Pro Gly Gly Arg Gln Phe 770 775 780 Gly Gly Gly Arg Arg Gly Gly Arg Gly Gly Gly Arg Ser Asn Arg Arg 785 790 795 800 Gln Thr Val Val Leu Gly Gly Gly Asp Lys Gln Val Pro Arg Gln Gln 805 810 815 His Gly Tyr His Leu Arg Ser Gly Ser Gly Gly Pro Ala Ala Ala Gln 820 825 830 Gln <210> 147 <211> 227 <212> PRT <213> Simian adenovirus 33 <400> 147 Met Ser Lys Glu Ile Pro Thr Pro Tyr Met Trp Ser Tyr Gln Pro Gln 1 5 10 15 Met Gly Leu Ala Ala Gly Ala Ser Gln Asp Tyr Ser Thr Arg Met Asn 20 25 30 Trp Leu Ser Ala Gly Pro Ser Met Ile Ser Arg Val Asn Asp Ile Leu 35 40 45 Ala Tyr Arg Asn Gln Leu Leu Leu Glu Gln Ser Ala Leu Thr Thr Thr 50 55 60 Pro Arg Gln His Leu Asn Pro Arg Asn Trp Pro Ala Ala Leu Val Tyr 65 70 75 80 Gln Glu Thr Pro Ala Pro Thr Thr Val Leu Leu Pro Arg Asp Ala Gln 85 90 95 Ala Glu Val Gln Met Thr Asn Ala Gly Val Gln Leu Ala Gly Gly Ser 100 105 110 Ala Leu Cys Arg His Arg Pro Arg Gln Ser Ile Lys Arg Leu Val Ile 115 120 125 Arg Gly Arg Gly Ile Gln Leu Asn Asp Glu Ser Val Ser Ser Ser Leu 130 135 140 Gly Leu Arg Pro Asp Gly Val Phe Gln Ile Ala Gly Cys Gly Arg Ser 145 150 155 160 Ser Phe Thr Pro Arg Gln Ala Val Leu Thr Leu Glu Ser Ser Ser Ser 165 170 175 Gln Pro Arg Ser Gly Gly Ile Gly Thr Leu Gln Phe Val Glu Glu Phe 180 185 190 Thr Pro Ser Val Tyr Phe Asn Pro Phe Ser Gly Ser Pro Gly Gln Tyr 195 200 205 Pro Asp Glu Phe Ile Pro Asn Phe Asp Ala Ile Ser Glu Ser Val Asp 210 215 220 Gly Tyr Asp 225 <210> 148 <211> 106 <212> PRT <213> Simian adenovirus 33 <400> 148 Met Ser Asn Gly Gly Ala Ala Glu Leu Ala Arg Leu Arg His Leu Asp 1 5 10 15 His Cys Arg Arg Phe Arg Cys Phe Ala Arg Glu Leu Thr Glu Phe Ile 20 25 30 Tyr Phe Glu Leu Pro Glu Glu His Pro Gln Gly Pro Ala His Gly Val 35 40 45 Arg Ile Thr Ile Glu Gly Gly Ile Asp Ser Arg Leu His Arg Ile Phe 50 55 60 Ser Gln Arg Pro Val Leu Ile Glu Arg Asp Gln Gly Asn Thr Thr Ile 65 70 75 80 Ser Ile Tyr Cys Ile Cys Asn His Pro Gly Leu His Glu Ser Leu Cys 85 90 95 Cys Leu Ile Cys Ala Glu Phe Asn Lys Asn 100 105 <210> 149 <211> 172 <212> PRT <213> Simian adenovirus 33 <400> 149 Met Gly Thr Leu Leu Val Val Leu Ala Leu Leu Ser Leu Leu Gly Leu 1 5 10 15 Gly Ser Ala Asn Leu Ile Pro Pro Asp His Asp Pro Cys Val Thr Phe 20 25 30 Asp Pro Glu Asn Cys Thr Leu Thr Phe Ala Pro Glu Thr Ser Arg Tyr 35 40 45 Cys Gly Val Val Ile Arg Cys Gly Leu Glu Cys Arg Pro Ile Glu Ile 50 55 60 Thr His Asn Asn Lys Thr Trp Asn Asn Thr Leu Phe Thr Thr Trp Gln 65 70 75 80 Pro Gly Tyr Pro Gln Trp Tyr Thr Val Ser Val Arg Gly Pro Asp Gly 85 90 95 Ser Val Arg Met Ala Asn Asn Thr Phe Ile Phe Ala Glu Met Cys Asp 100 105 110 Met Val Met Phe Met Ser Arg Gln Tyr Asp Leu Trp Pro Pro Ser Lys 115 120 125 Glu Asn Ile Val Ala Phe Ser Ile Val Tyr Cys Leu Gly Thr Cys Ile 130 135 140 Ile Thr Ala Ile Val Cys Val Cys Ile His Leu Leu Ile Val Ile Arg 145 150 155 160 Pro Arg Asn Ser Asn Glu Glu Lys Glu Lys Met Pro 165 170 <210> 150 <211> 188 <212> PRT <213> Simian adenovirus 33 <400> 150 Met Ile Phe Ile Thr Val Leu Leu Ala Ile Phe Asn Leu Leu Ser Ala 1 5 10 15 Ser His Gly Arg Thr His Val Thr Leu Thr Thr Gly Ser Thr Tyr Thr 20 25 30 Leu Lys Gly Pro Glu Gly His Asn Gly Val Ile Trp Trp Lys Leu Phe 35 40 45 Asp Asp Gly Gly Phe Val Ser Pro Cys Ser Thr Ser Asn Arg Tyr Leu 50 55 60 Cys Asn Gly Lys Asp Leu Thr Ile Ile Asn Val Thr Lys His Asp Asn 65 70 75 80 Gly Tyr Tyr Tyr Gly Thr Asn Tyr Ile Thr Ser Leu Asp Tyr Thr Ile 85 90 95 Thr Val Ile Ser Pro Thr Thr Pro Ala Pro Arg Lys Ile Thr Thr Phe 100 105 110 Ser Ser Ser Ser Ala Lys Asn Thr Ile Lys Ile Asn Thr Thr Ala Ile 115 120 125 Lys Met Leu Gln Lys Met Ala Ser Asn Tyr Thr Pro Pro Ala Thr Asn 130 135 140 Ala Leu Pro Lys Ser Ile Ile Gly Ile Ile Val Ala Ala Val Val Gly 145 150 155 160 Leu Ala Ile Ile Ile Ser Cys Ile Ile Tyr Tyr Ala Cys Cys Tyr Arg 165 170 175 Lys Ile Lys Gly Asp Pro Leu Leu Ser Phe Asp Ile 180 185 <210> 151 <211> 119 <212> PRT <213> Simian adenovirus 33 <400> 151 Met Lys Phe Leu Cys Val Leu Ala Phe Ser Val Phe Ser Phe Cys Thr 1 5 10 15 Ser Thr Pro Ile Thr Ile Val Asn Val Gln Thr Thr Leu Asn His Val 20 25 30 Asn Thr Thr Asn Tyr Thr Ser Thr Ser Tyr Ala Thr Ile His Thr Gln 35 40 45 Leu Ile Pro Phe Ser Thr Ile Lys Ala Asn Pro Gln Thr Lys Phe Ala 50 55 60 Leu Gln Leu Glu Ile Thr Ile Leu Ile Val Ile Gly Ile Thr Ile Leu 65 70 75 80 Ala Val Leu Leu Tyr Phe Ile Phe Cys Arg Gln Ile Pro Asn Val His 85 90 95 Lys Lys Pro Lys Arg Gln Pro Ile Tyr Cys Pro Met Ile Ser Lys Pro 100 105 110 His Leu Ala Leu Asn Glu Ile 115 <210> 152 <211> 91 <212> PRT <213> Simian adenovirus 33 <400> 152 Met Ile Pro Arg Asn Phe Phe Phe Thr Ile Leu Ile Cys Ala Phe Asn 1 5 10 15 Val Cys Ala Thr Phe Thr Ala Val Ala Thr Ala Thr Pro Asp Cys Ile 20 25 30 Gly Ala Phe Ala Ser Tyr Val Leu Phe Ala Phe Val Thr Cys Ile Cys 35 40 45 Val Cys Ser Ile Val Cys Leu Val Ile Asn Phe Phe Gln Leu Val Asp 50 55 60 Trp Ile Phe Val Arg Ile Ala Tyr Leu Arg His His Pro Glu Tyr Arg 65 70 75 80 Asn Gln Asn Val Ala Ala Leu Leu Arg Leu Ile 85 90 <210> 153 <211> 135 <212> PRT <213> Simian adenovirus 33 <400> 153 Met Thr Asp Gln Leu Asn Ala Ser Thr Ala Ala Val Asp Leu Leu Asp 1 5 10 15 Met Asp Gly Arg Thr Ser Glu Gln Arg Leu Ala Gln Leu Arg Ile Arg 20 25 30 Gln Gln Gln Glu Arg Ala Ala Lys Glu Leu Arg Asn Ala Ile Glu Ile 35 40 45 His Gln Cys Lys Lys Gly Ile Phe Cys Leu Val Lys Gln Ala Lys Ile 50 55 60 Ser Tyr Glu Ile Thr Ala Asn Asp His Arg Leu Ser Tyr Glu Leu Val 65 70 75 80 Gln Gln Arg Gln Lys Phe Thr Cys Met Val Gly Ile Asn Pro Ile Val 85 90 95 Ile Thr Gln Gln Ser Gly Asp Thr Lys Gly Cys Ile His Cys Ser Cys 100 105 110 Asp Ser Thr Glu Cys Ile Tyr Thr Leu Leu Lys Thr Leu Cys Gly Leu 115 120 125 Arg Asp Leu Leu Pro Met Asn 130 135 <210> 154 <211> 325 <212> PRT <213> Simian adenovirus 33 <400> 154 Met Ala Lys Arg Ala Arg Leu Ser Ser Ser Phe Asn Pro Val Tyr Pro 1 5 10 15 Tyr Glu Asp Glu Ser Ser Ser Gln His Pro Phe Ile Asn Pro Gly Phe 20 25 30 Ile Ser Pro Asp Gly Phe Thr Gln Ser Pro Asp Gly Val Leu Thr Leu 35 40 45 Lys Cys Val Ser Pro Leu Thr Thr Thr Ser Gly Ala Leu Asp Ile Lys 50 55 60 Val Gly Arg Gly Leu Lys Val Asp Ser Thr Asp Gly Ser Leu Glu Glu 65 70 75 80 Asn Ile Asp Ile Thr Ala Pro Leu Thr Lys Phe Asn His Ser Val Gly 85 90 95 Leu Ala Phe Gly Asp Gly Leu Glu Thr Lys Glu Asn Lys Leu Tyr Val 100 105 110 Lys Leu Gly Asp Gly Leu Lys Phe Ser Ser Gly Ser Ile Tyr Ile Asp 115 120 125 His Asp Val Asn Thr Leu Trp Thr Gly Val Asn Pro Ser Ala Asn Cys 130 135 140 Ile Ile Thr Asp Asn Gly Glu Thr Asn Asp Ser Lys Leu Thr Leu Ile 145 150 155 160 Leu Val Lys Ser Gly Gly Leu Ile Asn Ala Tyr Val Ser Leu Met Gly 165 170 175 Asp Ser Asp Thr Val Asn Lys Leu Thr Thr Glu Lys Ser Ala Gln Ile 180 185 190 Thr Val Asp Ile Tyr Phe Asp Asn Gln Gly Lys Val Leu Thr Glu Leu 195 200 205 Ser Ala Leu Lys Thr Asp Leu Lys His Lys Phe Gly Gln Asn Met Ala 210 215 220 Ser Ser Glu Val Ser Asn Cys Lys Gly Phe Met Pro Ser Leu Asn Ala 225 230 235 240 Tyr Pro Phe Arg Asn Pro Thr Lys Pro Thr Lys Gly Arg Glu Asp Tyr 245 250 255 Ile Tyr Gly Ile Thr Tyr Tyr Gln Ala Thr Asp Gly Asn Leu Tyr Glu 260 265 270 Leu Lys Thr Thr Ile Thr Leu Asn His Ser Val Ile Ser Ser Leu Cys 275 280 285 Ala Tyr Ala Met His Ile Ser Trp Ser Trp Asp Thr Val Thr Glu Pro 290 295 300 Glu Thr Thr Pro Thr Thr Leu Ile Thr Ser Pro Phe Ser Phe Ser Tyr 305 310 315 320 Ile Arg Glu Asp Asp 325 <210> 155 <211> 560 <212> DNA <213> Simian adenovirus 33 <220> <221> CDS <222> (10)..(552) <223> label=Elb\19K <400> 155 ctgccatcc atg gag gtt tgg gct atc ttg gaa gac ctt aga cag act agg 51 Met Glu Val Trp Ala Ile Leu Glu Asp Leu Arg Gln Thr Arg 1 5 10 cta ctg cta gaa aac gcc tcg gac gga gtc tct ggc ctt tgg aga ttc 99 Leu Leu Leu Glu Asn Ala Ser Asp Gly Val Ser Gly Leu Trp Arg Phe 15 20 25 30 tgg ttc ggt ggt gat cta gct agg cta gtc ttt agg ata aaa cag gac 147 Trp Phe Gly Gly Asp Leu Ala Arg Leu Val Phe Arg Ile Lys Gln Asp 35 40 45 tac agg gaa gaa ttt gaa aag tta ttg gac gac agt cca gga ctt ttt 195 Tyr Arg Glu Glu Phe Glu Lys Leu Leu Asp Asp Ser Pro Gly Leu Phe 50 55 60 gaa gct ctt aac ttg ggc cat cag gct cat ttt aag gag aag gtt tta 243 Glu Ala Leu Asn Leu Gly His Gln Ala His Phe Lys Glu Lys Val Leu 65 70 75 tca gtt tta gat ttt tct act cct ggt aga act gct gct gct gta gcc 291 Ser Val Leu Asp Phe Ser Thr Pro Gly Arg Thr Ala Ala Ala Val Ala 80 85 90 ttt ctt act ttt ata ttg gat aaa tgg atc cgc caa acc cac ttc agc 339 Phe Leu Thr Phe Ile Leu Asp Lys Trp Ile Arg Gln Thr His Phe Ser 95 100 105 110 aag gga tac gtt ttg gat ttc ata gca gca gct ttg tgg aga aca tgg 387 Lys Gly Tyr Val Leu Asp Phe Ile Ala Ala Ala Leu Trp Arg Thr Trp 115 120 125 aag gct cgc agg atg agg aca atc tta gat tac tgg cca gtg cag cct 435 Lys Ala Arg Arg Met Arg Thr Ile Leu Asp Tyr Trp Pro Val Gln Pro 130 135 140 ctg ggc gta gca ggg atc ctg aga cac cca ccg gcc atg cca gcg gtt 483 Leu Gly Val Ala Gly Ile Leu Arg His Pro Pro Ala Met Pro Ala Val 145 150 155 ctg gag gag gag cag cag gag gac aat ccg aga gcc ggc ctg gac cct 531 Leu Glu Glu Glu Gln Gln Glu Asp Asn Pro Arg Ala Gly Leu Asp Pro 160 165 170 ccg gtg gag gag gcg gag gag tagctgac 560 Pro Val Glu Glu Ala Glu Glu 175 180 <210> 156 <211> 181 <212> PRT <213> Simian adenovirus 33 <400> 156 Met Glu Val Trp Ala Ile Leu Glu Asp Leu Arg Gln Thr Arg Leu Leu 1 5 10 15 Leu Glu Asn Ala Ser Asp Gly Val Ser Gly Leu Trp Arg Phe Trp Phe 20 25 30 Gly Gly Asp Leu Ala Arg Leu Val Phe Arg Ile Lys Gln Asp Tyr Arg 35 40 45 Glu Glu Phe Glu Lys Leu Leu Asp Asp Ser Pro Gly Leu Phe Glu Ala 50 55 60 Leu Asn Leu Gly His Gln Ala His Phe Lys Glu Lys Val Leu Ser Val 65 70 75 80 Leu Asp Phe Ser Thr Pro Gly Arg Thr Ala Ala Ala Val Ala Phe Leu 85 90 95 Thr Phe Ile Leu Asp Lys Trp Ile Arg Gln Thr His Phe Ser Lys Gly 100 105 110 Tyr Val Leu Asp Phe Ile Ala Ala Ala Leu Trp Arg Thr Trp Lys Ala 115 120 125 Arg Arg Met Arg Thr Ile Leu Asp Tyr Trp Pro Val Gln Pro Leu Gly 130 135 140 Val Ala Gly Ile Leu Arg His Pro Pro Ala Met Pro Ala Val Leu Glu 145 150 155 160 Glu Glu Gln Gln Glu Asp Asn Pro Arg Ala Gly Leu Asp Pro Pro Val 165 170 175 Glu Glu Ala Glu Glu 180 <210> 157 <211> 5120 <212> DNA <213> Simian adenovirus 33 <220> <221> CDS <222> (2)..(604) <223> label=22K <220> <221> CDS <222> (1908)..(2357) <223> label=E3\CR1\alpha <220> <221> CDS <222> (2838)..(3422) <223> label=E3\CR1\beta <220> <221> CDS <222> (4677)..(5111) <223> label=E3\RID\beta <400> 157 g atg tcc cag cgc cga gga agc aag aag ctg aag gtg cag ctg ccg ccc 49 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 cca gag gat atg gag gaa gac tgg gac agt cag gca gag gaa gcg gag 97 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu Glu Ala Glu 20 25 30 gag atg gaa gat tgg gac agc cag gca gag gag gtg gac agc ctg gag 145 Glu Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Val Asp Ser Leu Glu 35 40 45 gaa gac agt ttg gag gag gaa gac gag gag gca gag gag gtg gaa gaa 193 Glu Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu 50 55 60 gca acc gcc gcc aaa cag ttg tcc tcg gcg gcg gag aca agc aag tcc 241 Ala Thr Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Ser 65 70 75 80 cca gac agc agc acg gct acc atc tcc gct ccg ggt cgg ggg gcc cag 289 Pro Asp Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Arg Gly Ala Gln 85 90 95 cgg cgg ccc aac agt aga tgg gac gag acc ggg cgc ttc ccg aac ccg 337 Arg Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe Pro Asn Pro 100 105 110 acc acc gct tcc aag acc ggt aag aag gag cga cag gga tac aag tcc 385 Thr Thr Ala Ser Lys Thr Gly Lys Lys Glu Arg Gln Gly Tyr Lys Ser 115 120 125 tgg cgg gga cat aaa aac gct atc atc tcc tgc ttg cat gaa tgc ggg 433 Trp Arg Gly His Lys Asn Ala Ile Ile Ser Cys Leu His Glu Cys Gly 130 135 140 ggc aac ata tcc ttc acc cgg cgc tac ctg ctc ttc cac cac ggg gtg 481 Gly Asn Ile Ser Phe Thr Arg Arg Tyr Leu Leu Phe His His Gly Val 145 150 155 160 aac ttc ccc cgc aat atc ttg cat tac tac cgt cac ctc cac agc ccc 529 Asn Phe Pro Arg Asn Ile Leu His Tyr Tyr Arg His Leu His Ser Pro 165 170 175 tac tgc agc cag caa gcc ccg gca acc tcg gca gaa aaa gac agc agc 577 Tyr Cys Ser Gln Gln Ala Pro Ala Thr Ser Ala Glu Lys Asp Ser Ser 180 185 190 ggc aac ggg gac cag aaa acc agc agt tagaaaatcc acagcaagtg 624 Gly Asn Gly Asp Gln Lys Thr Ser Ser 195 200 cagcaggagg aggactgagg atcacagcga acgagccagc gcagaccaga gagctgagga 684 atcggatctt tccaaccctc tatgccatct tccagcagag tcgggggcaa gagcaggaac 744 tgaaagtaaa aaaccgatct ctgcgctcgc tcaccagaag ttgtttgtat cacaagagcg 804 aagaccaact tcagcgcact ctcgaggacg ccgaggctct cttcaacaag tactgcgcgc 864 tgactcttaa agagtagccc ttgcccgcgc tcgctcgaaa aaggcgggaa tcacgtcacc 924 cttggcacct gtcctttgcc ctcgtcatga gtaaagaaat tcccacgcct tacatgtgga 984 gctatcagcc ccaaatgggg ttggcagcag gcgcctccca ggactactcc acccgcatga 1044 attggctcag cgccgggccc tcgatgatct cacgggttaa tgatatacta gcttatcgaa 1104 accagttact cctagaacag tcagctctca ccaccacacc ccgccaacac cttaatcccc 1164 ggaattggcc cgccgccctg gtgtaccagg aaactcccgc tcccaccacc gtactacttc 1224 ctcgagacgc ccaggccgaa gttcagatga ctaacgcagg tgtacagctg gcgggcggtt 1284 ccgccctgtg tcgtcaccgg cctcggcaga gtataaaacg cctggtgatc agaggccgag 1344 gtatccagct caacgacgag tcggtgagct cttcgcttgg tctgcgacca gacggagtct 1404 tccagatcgc cggctgtgga agatcttcct tcactcctcg tcaggctgtg ctgactttgg 1464 agagttcgtc ctcgcagccc cgctcgggcg gcatcgggac tctccagttc gtggaggagt 1524 ttactccctc tgtgtacttc aaccccttct ccggctctcc tggccagtac ccggacgagt 1584 tcataccgaa cttcgacgca atcagcgagt cagtggatgg ctatgattga tgtctaatgg 1644 tggcgcggct gagctagctc gactgcgaca tctagaccac tgccgccgct ttcgctgctt 1704 cgcccgggaa ctcaccgagt tcatctactt cgaactcccc gaggagcacc ctcaggggcc 1764 ggcccacgga gtgcggatta ccatcgaagg gggaatagac tctcgcctgc atcggatctt 1824 ctcccagcga cccgtgctga tcgagcgcga ccagggaaat acaaccatct ccatctactg 1884 catctgtaac caccccggat tgc atg aaa gcc ttt gct gtc tta ttt gtg ctg 1937 Met Lys Ala Phe Ala Val Leu Phe Val Leu 205 210 agt tta ata aaa act gag tta aga ccc tcc tac gga cta ccg ctt ctt 1985 Ser Leu Ile Lys Thr Glu Leu Arg Pro Ser Tyr Gly Leu Pro Leu Leu 215 220 225 caa cca gga ctt tac aac aac acc aac cag acc ctc cgt tcc agc cag 2033 Gln Pro Gly Leu Tyr Asn Asn Thr Asn Gln Thr Leu Arg Ser Ser Gln 230 235 240 aag acc cag acc ctt cct cct ctg atc cag gac tct aac tct acc ttc 2081 Lys Thr Gln Thr Leu Pro Pro Leu Ile Gln Asp Ser Asn Ser Thr Phe 245 250 255 cca gca cca tcc cct act aac ctt ccc gaa act aac aac ctc gga gct 2129 Pro Ala Pro Ser Pro Thr Asn Leu Pro Glu Thr Asn Asn Leu Gly Ala 260 265 270 275 caa ctg caa cac cgc ctt tcc cga agc ctc ctt tct gcc aat act acc 2177 Gln Leu Gln His Arg Leu Ser Arg Ser Leu Leu Ser Ala Asn Thr Thr 280 285 290 act ccc aaa acc gga ggt gag ctc cgc ggt ctc ccc act gac gac ccc 2225 Thr Pro Lys Thr Gly Gly Glu Leu Arg Gly Leu Pro Thr Asp Asp Pro 295 300 305 tgg gtg gta gcg ggt ttt gta acg tta gga gta gtt gcg ggt ggg ctt 2273 Trp Val Val Ala Gly Phe Val Thr Leu Gly Val Val Ala Gly Gly Leu 310 315 320 gtg ctg atc ctt tgc tac cta tac aca cct tgc tgt gca tat tta gtt 2321 Val Leu Ile Leu Cys Tyr Leu Tyr Thr Pro Cys Cys Ala Tyr Leu Val 325 330 335 ata ttg tgc tgc tgg ttt aag aaa tgg gga ccc tac tagtcgtgct 2367 Ile Leu Cys Cys Trp Phe Lys Lys Trp Gly Pro Tyr 340 345 350 tgctttactt tcgcttttgg gactgggctc tgctaatctc attcctcctg atcacgatcc 2427 atgtgtgaca tttgatccag aaaactgcac actcaccttt gcacctgaaa caagccgcta 2487 ctgcggagta gttattaggt gcggactgga atgcaggccc attgaaatta cacacaataa 2547 caaaacttgg aacaatacat tattcaccac atggcaacca ggatatcctc agtggtatac 2607 tgtctctgtc cggggtcctg acggttccgt ccgcatggct aataacactt tcatttttgc 2667 tgaaatgtgc gatatggtca tgtttatgag cagacagtat gacctatggc ctcccagcaa 2727 agaaaacatt gtggcattct ccattgttta ttgcttggga acatgcatca tcactgctat 2787 cgtgtgtgtg tgcatacact tgcttatagt cattcgcccc agaaacagca atg agg 2843 Met Arg aaa aag aga aaa tgc cct aac ttt ttt cac aac ttt ttt tca gcc atg 2891 Lys Lys Arg Lys Cys Pro Asn Phe Phe His Asn Phe Phe Ser Ala Met 355 360 365 cct tca gct ttt ttt ctt ctt act att gtt gct gtt att tcc gca caa 2939 Pro Ser Ala Phe Phe Leu Leu Thr Ile Val Ala Val Ile Ser Ala Gln 370 375 380 385 aca ata gta gat gtt cca ctt ggt tct aac tac aca cta ata ggt cct 2987 Thr Ile Val Asp Val Pro Leu Gly Ser Asn Tyr Thr Leu Ile Gly Pro 390 395 400 aca atc cat tca gaa gtt acc tgg tgc agg ctt aat act gaa gac tac 3035 Thr Ile His Ser Glu Val Thr Trp Cys Arg Leu Asn Thr Glu Asp Tyr 405 410 415 tat aat gta ttt tgt gat ggg gat gat gac att caa gta acc tgt aac 3083 Tyr Asn Val Phe Cys Asp Gly Asp Asp Asp Ile Gln Val Thr Cys Asn 420 425 430 aaa cag aat ctt aca ctc att aat gtt acc aaa agt tac aat ggt tac 3131 Lys Gln Asn Leu Thr Leu Ile Asn Val Thr Lys Ser Tyr Asn Gly Tyr 435 440 445 tat tat gga tat gat aga tct ggc agt gaa ttt aaa aat tac ctg gta 3179 Tyr Tyr Gly Tyr Asp Arg Ser Gly Ser Glu Phe Lys Asn Tyr Leu Val 450 455 460 465 cga aca att cca ccc att aca aac att aaa ata gag aaa ctc caa atg 3227 Arg Thr Ile Pro Pro Ile Thr Asn Ile Lys Ile Glu Lys Leu Gln Met 470 475 480 gat agt gac att tta agt aat ctt aca ata tcc ccc acc aca cca tct 3275 Asp Ser Asp Ile Leu Ser Asn Leu Thr Ile Ser Pro Thr Thr Pro Ser 485 490 495 gaa caa aac att cca agt tca atg att gca att att gcg gcg gtg gca 3323 Glu Gln Asn Ile Pro Ser Ser Met Ile Ala Ile Ile Ala Ala Val Ala 500 505 510 gtg gga atg gca atc ata ata aca tgt atg att gtt tat gct tgc tgc 3371 Val Gly Met Ala Ile Ile Ile Thr Cys Met Ile Val Tyr Ala Cys Cys 515 520 525 tac aag aaa atc agg cgt gaa aaa caa gat tca cta cta aat tat gat 3419 Tyr Lys Lys Ile Arg Arg Glu Lys Gln Asp Ser Leu Leu Asn Tyr Asp 530 535 540 545 ttt taacttctta ttttaacaga caatgatttt cattacagtt cttcttgcca 3472 Phe tctttaactt actatcagcc tctcatgggc gcacacatgt cactctaact actggttcca 3532 catacacact aaaaggccca gaaggtcata atggtgttat ttggtggaaa ctatttgatg 3592 atggagggtt tgttagtccc tgcagcacat ctaatagata tttatgtaat ggtaaagacc 3652 taactattat taatgtcaca aaacacgaca atggctacta ttatgggacc aattatatta 3712 caagtttaga ttacaccatt actgtcatat cgcctactac accagcaccg cgcaaaatca 3772 caactttctc tagcagcagc gctaaaaaca caatcaaaat taatacaact gctataaaaa 3832 tgctccaaaa aatggcttct aattataccc cacccgctac caatgcgctt cctaaatcaa 3892 ttattggaat aattgtagcg gcggtagtgg ggctggcaat tattatttct tgcataattt 3952 attatgcctg ctgctataga aaaataaaag gagaccccct actaagcttt gatatttaat 4012 tttttttcat agcaccatga aattcctatg tgtattagct ttttcagttt ttagcttttg 4072 cacatccacc cccatcacca ttgtcaatgt gcagactact ttaaatcatg ttaatactac 4132 aaattacaca tctacctcct atgcaaccat acatacccag cttattcctt tttccacaat 4192 taaagccaat cctcagacta aatttgcact acaactagaa atcactatcc taattgtgat 4252 tggaataact attctagctg ttcttcttta ttttatattc tgccgccaaa tacccaatgt 4312 tcataaaaaa ccaaaaagac aacccattta ttgtcctatg attagtaaac ctcacttggc 4372 cttaaatgaa atctaaggtc tattcttttc ttttttacag tatggtgatc accaatcatg 4432 atccctagaa atttcttctt caccatactc atctgtgctt tcaatgtctg tgctaccttc 4492 accgcagtag ccactgcaac cccagactgt ataggagcat ttgcttccta tgtacttttt 4552 gcctttgtta cttgcatctg cgtgtgtagc atagtctgcc tggttattaa ttttttccaa 4612 cttgtagact ggatctttgt acgaattgcc tacctgcgtc accatcccga ataccgcaat 4672 caaa atg ttg cgg cac ttc tta ggc tta ttt aaa acc atg cag gct atg 4721 Met Leu Arg His Phe Leu Gly Leu Phe Lys Thr Met Gln Ala Met 550 555 560 cta cca gtc att cta att ctg ctg cta ccc tgc gtt gcc tta gct ccc 4769 Leu Pro Val Ile Leu Ile Leu Leu Leu Pro Cys Val Ala Leu Ala Pro 565 570 575 aca acc act cgc act cca cct gaa caa ctt aga aaa tgc aaa ttt caa 4817 Thr Thr Thr Arg Thr Pro Pro Glu Gln Leu Arg Lys Cys Lys Phe Gln 580 585 590 caa cca tgg tca ttc ctt gat tgc tac cat gaa aaa tct gat ttt ccc 4865 Gln Pro Trp Ser Phe Leu Asp Cys Tyr His Glu Lys Ser Asp Phe Pro 595 600 605 aca tac tgg ata gtg att gtt gga ata att aac ata ctc tca tgt acc 4913 Thr Tyr Trp Ile Val Ile Val Gly Ile Ile Asn Ile Leu Ser Cys Thr 610 615 620 625 tta ttc tca ttc cta ata tac ccc ata ttt agt ttt ggg tgg aat gct 4961 Leu Phe Ser Phe Leu Ile Tyr Pro Ile Phe Ser Phe Gly Trp Asn Ala 630 635 640 ccc aat gca ctg ggt tac cca caa att cca gag gaa cac att gca cta 5009 Pro Asn Ala Leu Gly Tyr Pro Gln Ile Pro Glu Glu His Ile Ala Leu 645 650 655 cag aac atg caa cag cca cta gat cta ata gat tat gaa aat gag cca 5057 Gln Asn Met Gln Gln Pro Leu Asp Leu Ile Asp Tyr Glu Asn Glu Pro 660 665 670 cag cct cca cta ctc cct gcc att agc tac ttc aac cta acc ggt gga 5105 Gln Pro Pro Leu Leu Pro Ala Ile Ser Tyr Phe Asn Leu Thr Gly Gly 675 680 685 gat gac tgatcagct 5120 Asp Asp 690 <210> 158 <211> 201 <212> PRT <213> Simian adenovirus 33 <400> 158 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu Glu Ala Glu 20 25 30 Glu Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Val Asp Ser Leu Glu 35 40 45 Glu Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu 50 55 60 Ala Thr Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Ser 65 70 75 80 Pro Asp Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Arg Gly Ala Gln 85 90 95 Arg Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe Pro Asn Pro 100 105 110 Thr Thr Ala Ser Lys Thr Gly Lys Lys Glu Arg Gln Gly Tyr Lys Ser 115 120 125 Trp Arg Gly His Lys Asn Ala Ile Ile Ser Cys Leu His Glu Cys Gly 130 135 140 Gly Asn Ile Ser Phe Thr Arg Arg Tyr Leu Leu Phe His His Gly Val 145 150 155 160 Asn Phe Pro Arg Asn Ile Leu His Tyr Tyr Arg His Leu His Ser Pro 165 170 175 Tyr Cys Ser Gln Gln Ala Pro Ala Thr Ser Ala Glu Lys Asp Ser Ser 180 185 190 Gly Asn Gly Asp Gln Lys Thr Ser Ser 195 200 <210> 159 <211> 150 <212> PRT <213> Simian adenovirus 33 <400> 159 Met Lys Ala Phe Ala Val Leu Phe Val Leu Ser Leu Ile Lys Thr Glu 1 5 10 15 Leu Arg Pro Ser Tyr Gly Leu Pro Leu Leu Gln Pro Gly Leu Tyr Asn 20 25 30 Asn Thr Asn Gln Thr Leu Arg Ser Ser Gln Lys Thr Gln Thr Leu Pro 35 40 45 Pro Leu Ile Gln Asp Ser Asn Ser Thr Phe Pro Ala Pro Ser Pro Thr 50 55 60 Asn Leu Pro Glu Thr Asn Asn Leu Gly Ala Gln Leu Gln His Arg Leu 65 70 75 80 Ser Arg Ser Leu Leu Ser Ala Asn Thr Thr Thr Pro Lys Thr Gly Gly 85 90 95 Glu Leu Arg Gly Leu Pro Thr Asp Asp Pro Trp Val Val Ala Gly Phe 100 105 110 Val Thr Leu Gly Val Val Ala Gly Gly Leu Val Leu Ile Leu Cys Tyr 115 120 125 Leu Tyr Thr Pro Cys Cys Ala Tyr Leu Val Ile Leu Cys Cys Trp Phe 130 135 140 Lys Lys Trp Gly Pro Tyr 145 150 <210> 160 <211> 195 <212> PRT <213> Simian adenovirus 33 <400> 160 Met Arg Lys Lys Arg Lys Cys Pro Asn Phe Phe His Asn Phe Phe Ser 1 5 10 15 Ala Met Pro Ser Ala Phe Phe Leu Leu Thr Ile Val Ala Val Ile Ser 20 25 30 Ala Gln Thr Ile Val Asp Val Pro Leu Gly Ser Asn Tyr Thr Leu Ile 35 40 45 Gly Pro Thr Ile His Ser Glu Val Thr Trp Cys Arg Leu Asn Thr Glu 50 55 60 Asp Tyr Tyr Asn Val Phe Cys Asp Gly Asp Asp Asp Ile Gln Val Thr 65 70 75 80 Cys Asn Lys Gln Asn Leu Thr Leu Ile Asn Val Thr Lys Ser Tyr Asn 85 90 95 Gly Tyr Tyr Tyr Gly Tyr Asp Arg Ser Gly Ser Glu Phe Lys Asn Tyr 100 105 110 Leu Val Arg Thr Ile Pro Pro Ile Thr Asn Ile Lys Ile Glu Lys Leu 115 120 125 Gln Met Asp Ser Asp Ile Leu Ser Asn Leu Thr Ile Ser Pro Thr Thr 130 135 140 Pro Ser Glu Gln Asn Ile Pro Ser Ser Met Ile Ala Ile Ile Ala Ala 145 150 155 160 Val Ala Val Gly Met Ala Ile Ile Ile Thr Cys Met Ile Val Tyr Ala 165 170 175 Cys Cys Tyr Lys Lys Ile Arg Arg Glu Lys Gln Asp Ser Leu Leu Asn 180 185 190 Tyr Asp Phe 195 <210> 161 <211> 145 <212> PRT <213> Simian adenovirus 33 <400> 161 Met Leu Arg His Phe Leu Gly Leu Phe Lys Thr Met Gln Ala Met Leu 1 5 10 15 Pro Val Ile Leu Ile Leu Leu Leu Pro Cys Val Ala Leu Ala Pro Thr 20 25 30 Thr Thr Arg Thr Pro Pro Glu Gln Leu Arg Lys Cys Lys Phe Gln Gln 35 40 45 Pro Trp Ser Phe Leu Asp Cys Tyr His Glu Lys Ser Asp Phe Pro Thr 50 55 60 Tyr Trp Ile Val Ile Val Gly Ile Ile Asn Ile Leu Ser Cys Thr Leu 65 70 75 80 Phe Ser Phe Leu Ile Tyr Pro Ile Phe Ser Phe Gly Trp Asn Ala Pro 85 90 95 Asn Ala Leu Gly Tyr Pro Gln Ile Pro Glu Glu His Ile Ala Leu Gln 100 105 110 Asn Met Gln Gln Pro Leu Asp Leu Ile Asp Tyr Glu Asn Glu Pro Gln 115 120 125 Pro Pro Leu Leu Pro Ala Ile Ser Tyr Phe Asn Leu Thr Gly Gly Asp 130 135 140 Asp 145 <210> 162 <211> 880 <212> DNA <213> Simian adenovirus 33 <220> <221> CDS <222> (1)..(580) <223> label=Ela <220> <221> CDS <222> (677)..(879) <223> label=Ela <400> 162 atg aga cac ctg cga ttc ctg cca cag gag att atc tcc agc gag acc 48 Met Arg His Leu Arg Phe Leu Pro Gln Glu Ile Ile Ser Ser Glu Thr 1 5 10 15 ggg atc gaa ata ctg gag ttc gtg gta aat acc ctg atg gga gac gat 96 Gly Ile Glu Ile Leu Glu Phe Val Val Asn Thr Leu Met Gly Asp Asp 20 25 30 ccg gag ccg ccc gtg cag cct ttc gat cca cct acg ctt cac gaa ctg 144 Pro Glu Pro Pro Val Gln Pro Phe Asp Pro Pro Thr Leu His Glu Leu 35 40 45 tat gat tta gag gta gac ggg ccg gag gat ccc aat gag gaa gct gtg 192 Tyr Asp Leu Glu Val Asp Gly Pro Glu Asp Pro Asn Glu Glu Ala Val 50 55 60 aat ggg ttt ttt act gat tct atg ctg cta gct gct gag gaa gga ttg 240 Asn Gly Phe Phe Thr Asp Ser Met Leu Leu Ala Ala Glu Glu Gly Leu 65 70 75 80 gac gta aac cct cct ccg gag acc ctt gat acc cca ggg gtg gtt gtg 288 Asp Val Asn Pro Pro Pro Glu Thr Leu Asp Thr Pro Gly Val Val Val 85 90 95 gaa agc ggc aga ggt ggg aaa aaa ttg cct gat ctg gga gca gct gaa 336 Glu Ser Gly Arg Gly Gly Lys Lys Leu Pro Asp Leu Gly Ala Ala Glu 100 105 110 atg gac ttg cgt tgt tat gaa gag ggt ttt cct ccg agt gat gat gaa 384 Met Asp Leu Arg Cys Tyr Glu Glu Gly Phe Pro Pro Ser Asp Asp Glu 115 120 125 gat gag gaa agt gag cag tcc atc cag acc gca gtg aat gag gga gtg 432 Asp Glu Glu Ser Glu Gln Ser Ile Gln Thr Ala Val Asn Glu Gly Val 130 135 140 aaa gct gcc agt gat gtt ttt aag ttg gac tgt ccg gag ctg cct gga 480 Lys Ala Ala Ser Asp Val Phe Lys Leu Asp Cys Pro Glu Leu Pro Gly 145 150 155 160 cat ggc tgt aag tct tgt gaa ttt cac agg aat aac act gga atg aaa 528 His Gly Cys Lys Ser Cys Glu Phe His Arg Asn Asn Thr Gly Met Lys 165 170 175 gaa cta tta tgc tcg ctt tgc tat atg aga acg cac tgc cac ttt att 576 Glu Leu Leu Cys Ser Leu Cys Tyr Met Arg Thr His Cys His Phe Ile 180 185 190 tac a gtaagtgtgt ttaagtgaaa tttaaaggaa cagtgaagct gttttaataa 630 Tyr ctttgttgaa tgggggattt atgttttact tgtgattttt ttatag gt cct gtg 684 Ser Pro Val 195 tct gat gat gat tcg cct tct cct gat tca act acc tca cct cct gaa 732 Ser Asp Asp Asp Ser Pro Ser Pro Asp Ser Thr Thr Ser Pro Pro Glu 200 205 210 att cag gcg ccc gtc cct gca aac gta tgc aag ccc att cct gtg aag 780 Ile Gln Ala Pro Val Pro Ala Asn Val Cys Lys Pro Ile Pro Val Lys 215 220 225 cct aag cct ggg aaa cgc cct gct gtg gat aag ctt gag gac ttg ttg 828 Pro Lys Pro Gly Lys Arg Pro Ala Val Asp Lys Leu Glu Asp Leu Leu 230 235 240 gag ggt ggg gat gga cct ttg gac ttt agt acc cgg aaa ctg cca agg 876 Glu Gly Gly Asp Gly Pro Leu Asp Phe Ser Thr Arg Lys Leu Pro Arg 245 250 255 260 caa t 880 Gln <210> 163 <211> 261 <212> PRT <213> Simian adenovirus 33 <400> 163 Met Arg His Leu Arg Phe Leu Pro Gln Glu Ile Ile Ser Ser Glu Thr 1 5 10 15 Gly Ile Glu Ile Leu Glu Phe Val Val Asn Thr Leu Met Gly Asp Asp 20 25 30 Pro Glu Pro Pro Val Gln Pro Phe Asp Pro Pro Thr Leu His Glu Leu 35 40 45 Tyr Asp Leu Glu Val Asp Gly Pro Glu Asp Pro Asn Glu Glu Ala Val 50 55 60 Asn Gly Phe Phe Thr Asp Ser Met Leu Leu Ala Ala Glu Glu Gly Leu 65 70 75 80 Asp Val Asn Pro Pro Pro Glu Thr Leu Asp Thr Pro Gly Val Val Val 85 90 95 Glu Ser Gly Arg Gly Gly Lys Lys Leu Pro Asp Leu Gly Ala Ala Glu 100 105 110 Met Asp Leu Arg Cys Tyr Glu Glu Gly Phe Pro Pro Ser Asp Asp Glu 115 120 125 Asp Glu Glu Ser Glu Gln Ser Ile Gln Thr Ala Val Asn Glu Gly Val 130 135 140 Lys Ala Ala Ser Asp Val Phe Lys Leu Asp Cys Pro Glu Leu Pro Gly 145 150 155 160 His Gly Cys Lys Ser Cys Glu Phe His Arg Asn Asn Thr Gly Met Lys 165 170 175 Glu Leu Leu Cys Ser Leu Cys Tyr Met Arg Thr His Cys His Phe Ile 180 185 190 Tyr Ser Pro Val Ser Asp Asp Asp Ser Pro Ser Pro Asp Ser Thr Thr 195 200 205 Ser Pro Pro Glu Ile Gln Ala Pro Val Pro Ala Asn Val Cys Lys Pro 210 215 220 Ile Pro Val Lys Pro Lys Pro Gly Lys Arg Pro Ala Val Asp Lys Leu 225 230 235 240 Glu Asp Leu Leu Glu Gly Gly Asp Gly Pro Leu Asp Phe Ser Thr Arg 245 250 255 Lys Leu Pro Arg Gln 260 <210> 164 <211> 890 <212> DNA <213> Simian adenovirus 33 <220> <221> CDS <222> (12)..(366) <223> label=33K <220> <221> CDS <222> (536)..(888) <223> label=33K <400> 164 gttcccccag g atg tcc cag cgc cga gga agc aag aag ctg aag gtg cag 50 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln 1 5 10 ctg ccg ccc cca gag gat atg gag gaa gac tgg gac agt cag gca gag 98 Leu Pro Pro Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu 15 20 25 gaa gcg gag gag atg gaa gat tgg gac agc cag gca gag gag gtg gac 146 Glu Ala Glu Glu Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Val Asp 30 35 40 45 agc ctg gag gaa gac agt ttg gag gag gaa gac gag gag gca gag gag 194 Ser Leu Glu Glu Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu 50 55 60 gtg gaa gaa gca acc gcc gcc aaa cag ttg tcc tcg gcg gcg gag aca 242 Val Glu Glu Ala Thr Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr 65 70 75 agc aag tcc cca gac agc agc acg gct acc atc tcc gct ccg ggt cgg 290 Ser Lys Ser Pro Asp Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Arg 80 85 90 ggg gcc cag cgg cgg ccc aac agt aga tgg gac gag acc ggg cgc ttc 338 Gly Ala Gln Arg Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe 95 100 105 ccg aac ccg acc acc gct tcc aag acc g gtaagaagga gcgacaggga 386 Pro Asn Pro Thr Thr Ala Ser Lys Thr 110 115 tacaagtcct ggcggggaca taaaaacgct atcatctcct gcttgcatga atgcgggggc 446 aacatatcct tcacccggcg ctacctgctc ttccaccacg gggtgaactt cccccgcaat 506 atcttgcatt actaccgtca cctccacag cc cct act gca gcc agc aag ccc 558 Ala Pro Thr Ala Ala Ser Lys Pro 120 125 cgg caa cct cgg cag aaa aag aca gca gcg gca acg ggg acc aga aaa 606 Arg Gln Pro Arg Gln Lys Lys Thr Ala Ala Ala Thr Gly Thr Arg Lys 130 135 140 cca gca gtt aga aaa tcc aca gca agt gca gca gga gga gga ctg agg 654 Pro Ala Val Arg Lys Ser Thr Ala Ser Ala Ala Gly Gly Gly Leu Arg 145 150 155 atc aca gcg aac gag cca gcg cag acc aga gag ctg agg aat cgg atc 702 Ile Thr Ala Asn Glu Pro Ala Gln Thr Arg Glu Leu Arg Asn Arg Ile 160 165 170 ttt cca acc ctc tat gcc atc ttc cag cag agt cgg ggg caa gag cag 750 Phe Pro Thr Leu Tyr Ala Ile Phe Gln Gln Ser Arg Gly Gln Glu Gln 175 180 185 190 gaa ctg aaa gta aaa aac cga tct ctg cgc tcg ctc acc aga agt tgt 798 Glu Leu Lys Val Lys Asn Arg Ser Leu Arg Ser Leu Thr Arg Ser Cys 195 200 205 ttg tat cac aag agc gaa gac caa ctt cag cgc act ctc gag gac gcc 846 Leu Tyr His Lys Ser Glu Asp Gln Leu Gln Arg Thr Leu Glu Asp Ala 210 215 220 gag gct ctc ttc aac aag tac tgc gcg ctg act ctt aaa gag ta 890 Glu Ala Leu Phe Asn Lys Tyr Cys Ala Leu Thr Leu Lys Glu 225 230 235 <210> 165 <211> 236 <212> PRT <213> Simian adenovirus 33 <400> 165 Met Ser Gln Arg Arg Gly Ser Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Ala Glu Glu Ala Glu 20 25 30 Glu Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Val Asp Ser Leu Glu 35 40 45 Glu Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu 50 55 60 Ala Thr Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Ser 65 70 75 80 Pro Asp Ser Ser Thr Ala Thr Ile Ser Ala Pro Gly Arg Gly Ala Gln 85 90 95 Arg Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Phe Pro Asn Pro 100 105 110 Thr Thr Ala Ser Lys Thr Ala Pro Thr Ala Ala Ser Lys Pro Arg Gln 115 120 125 Pro Arg Gln Lys Lys Thr Ala Ala Ala Thr Gly Thr Arg Lys Pro Ala 130 135 140 Val Arg Lys Ser Thr Ala Ser Ala Ala Gly Gly Gly Leu Arg Ile Thr 145 150 155 160 Ala Asn Glu Pro Ala Gln Thr Arg Glu Leu Arg Asn Arg Ile Phe Pro 165 170 175 Thr Leu Tyr Ala Ile Phe Gln Gln Ser Arg Gly Gln Glu Gln Glu Leu 180 185 190 Lys Val Lys Asn Arg Ser Leu Arg Ser Leu Thr Arg Ser Cys Leu Tyr 195 200 205 His Lys Ser Glu Asp Gln Leu Gln Arg Thr Leu Glu Asp Ala Glu Ala 210 215 220 Leu Phe Asn Lys Tyr Cys Ala Leu Thr Leu Lys Glu 225 230 235 <210> 166 <211> 35606 <212> DNA <213> Simian adenovirus 35 <220> <221> repeat_region <222> (1)..(126) <223> label=ITR <220> <221> CDS <222> (1911)..(3395) <223> label=E1b\55K <220> <221> CDS <222> (3491)..(3904) <223> label=pIX <220> <221> misc_feature <222> (3965)..(5586) <223> complement (3965..5295, 5574..5586) label=IVa2 <220> <221> misc_feature <222> (5068)..(13877) <223> complement (5068..8643, 13869..13877) label=pol <220> <221> misc_feature <222> (8445)..(13877) <223> complement (8445..10418, 13869..13877) label=pTP <220> <221> CDS <222> (10886)..(12052) <223> label=52K <220> <221> CDS <222> (12083)..(13843) <223> label=pIIIa <220> <221> CDS <222> (13919)..(15607) <223> label=penton <220> <221> CDS <222> (15621)..(16196) <223> label=pVII <220> <221> CDS <222> (16239)..(17297) <223> label=V <220> <221> CDS <222> (17329)..(17556) <223> label=pX <220> <221> CDS <222> (17632)..(18381) <223> label=pVI <220> <221> CDS <222> (18510)..(21377) <223> label=hexon <220> <221> misc_feature <222> (22116)..(23663) <223> complement label=DBP <220> <221> CDS <222> (23694)..(26177) <223> label=100K <220> <221> CDS <222> (26824)..(27504) <223> label=pVIII <220> <221> CDS <222> (27507)..(27824) <223> label=E3\12.5K <220> <221> CDS <222> (28206)..(28721) <223> label=E3\gp19K <220> <221> CDS <222> (29435)..(29872) <223> label=E3\CR1\gamma <220> <221> CDS <222> (29891)..(30190) <223> label=E3\CR1\delta <220> <221> CDS <222> (30234)..(30506) <223> label=E3\RID\alpha <220> <221> CDS <222> (30911)..(31315) <223> label=E3\14.7K <220> <221> CDS <222> (31550)..(32608) <223> label=fiber <220> <221> misc_feature <222> (32654)..(33825) <223> complement (32654..32902, 33613..33825) label=E4\orf\6/7 <220> <221> misc_feature <222> (32902)..(33825) <223> complement label=E4\orf6 <220> <221> misc_feature <222> (33701)..(34081) <223> complement label=E4\orf4 <220> <221> misc_feature <222> (34095)..(34445) <223> complement label=E4\orf3 <220> <221> misc_feature <222> (34445)..(34831) <223> complement label=E4\orf2 <220> <221> misc_feature <222> (34873)..(35244) <223> complement label=E4\orf1 <220> <221> repeat_region <222> (35481)..(35606) <223> complement label=ITR <400> 166 catcatcaat aatatacctt atagatggaa tggtgccaat atgtaaatga ggtggtttga 60 aaatggagag cggaagggga ttggcttggg gttcaacggt cacggggcgg cgcgggaagg 120 tgacgtatgc gtgggtgtgg ctaagatgca agctgtcgcg gtatttctga cgtaaacgag 180 gtggagttta aacacggaag tacacagttt cccgcgctta ttgacaggaa atgaggtagt 240 tttgggcgga tgcaagtgaa aattcctcat tttcgcgcga aaactgaatg aggaagtgaa 300 tatctgagta atttcgtgtt tatgacaggg tggagtattt accgagggcc gagtagactt 360 tgaccgatta cgtggaggtt tcgattaccg tgtttttcac ctaaatttcc gcgtacggtg 420 tcaaagtcct gtgtttttac gtaggtgtca gctgatcgcc agggtattta aacctgacga 480 gttccgtcaa gaggccactc ttgagtgcca gcgagaagag ttttctcctc cgcgctgcga 540 gtcagatctc cacttcgaaa atgagacacc tgcgtttcct gtcccaggag atagtctcca 600 ctgaaactgg gaatgaaata ctgcagtttg tggtaaatac tctgatggga gacgatccag 660 agccgcctga gccatctttt gatcctccta cgcttcatga attatatgat ttagaggtag 720 acggaccgga ggaccctaat gaggacgacg tgaatgggtt ttttactgat tctatgttat 780 tagctgctaa tgagggagtg gatttagacc caccttctgg aactcttgat actccagggg 840 tgattgtgga aagcgacata aatgggaaaa atttacctga tttgggtgct gctgaattgg 900 acttgcactg ctatgaagag ggttttcctc cgagtgatga tgaagatgtg gagaatgagc 960 agtcaattca gaccgcagcg ggtgagggag tgaaagcagc cagtgatggt tttaagttgg 1020 actgcccgat gctgcctgga catggctgta agtcttgtga atttcacagg aaaaatactg 1080 gagtaaaaga aatattatgc tcgctttgtt atatgagagc gcattgccac tttatttaca 1140 gtaagtgtgt ttaagttaaa tttaaaggaa cagtagctgt ttttataact cttggatggg 1200 tgatttatgt tttgcttgtg attttttata ggtcctgtgt ctgatgctga tgaatcgcct 1260 tctcctgatt caactacctc acctcctgaa attcaggcac ccgtccctgc aaatgtatgc 1320 aagcccattc ctgtgaagct taagcctggg aaacgccctg ctgtggataa acttgaggat 1380 ttgctggagg gtgtggatga acctttggac ttgtgtaccc ggaaaatacc aaggcaatga 1440 gtgccccgca cctgtgttta tttaatgacg tcactattta tgtgagagtg ccatgtaata 1500 aaattatgtc agctgctgag tgttttattg tttcttgggt gggacttggg atatataagt 1560 aggagcagac ctgtgtggtt agctcacagc agcttgcttc catccatgga ggtttgggcc 1620 atcttggaag atcttaggca gactaggcaa ctgctagaaa acgcctcgga cggagtctct 1680 ggtctttgga gattctggtt cggtggtgat ctggctagac tagtctttag aataaaacag 1740 gattacaggc aagaatttga aaagttattg gacgactgtt caggactttt tgaagctctt 1800 aacttgggcc accaggctca ttttaaggag aaggttttat cagttttgga tttttctacc 1860 cctggtagaa ctgctgctgc tgtagctttc cttacattca tatttgataa atg gat 1916 Met Asp 1 ccc aca gac cca ctt cag caa ggg ata cgt ttt gga ttt cat agc agc 1964 Pro Thr Asp Pro Leu Gln Gln Gly Ile Arg Phe Gly Phe His Ser Ser 5 10 15 agc ttt gtg gag aac atg gaa ggc tcg cag gat gag gac aat ctt aga 2012 Ser Phe Val Glu Asn Met Glu Gly Ser Gln Asp Glu Asp Asn Leu Arg 20 25 30 tta ctg gcc agt aca gcc tct ggg cgt agc agg gat cct gag aca ccc 2060 Leu Leu Ala Ser Thr Ala Ser Gly Arg Ser Arg Asp Pro Glu Thr Pro 35 40 45 50 acc gac cat gcc agc ggt ttt gga gga gga gca cca aga gga caa tcc 2108 Thr Asp His Ala Ser Gly Phe Gly Gly Gly Ala Pro Arg Gly Gln Ser 55 60 65 gag agt cgg cct gga ccc tcc ggt gga gga ggc gga gga gta gct gac 2156 Glu Ser Arg Pro Gly Pro Ser Gly Gly Gly Gly Gly Gly Val Ala Asp 70 75 80 ttg ttt cct gaa ctg cga cgg gtg ctt act aga tct aca acc agt gga 2204 Leu Phe Pro Glu Leu Arg Arg Val Leu Thr Arg Ser Thr Thr Ser Gly 85 90 95 cgg gac agg ggc att aag agg gaa agg aat cct agt gga act aat ccc 2252 Arg Asp Arg Gly Ile Lys Arg Glu Arg Asn Pro Ser Gly Thr Asn Pro 100 105 110 aga tct gag ttg gct tta agt ttg atg agt cgc aga cgt cct gaa act 2300 Arg Ser Glu Leu Ala Leu Ser Leu Met Ser Arg Arg Arg Pro Glu Thr 115 120 125 130 ata tgg tgg cat gag gtt cag aat gag ggc agg gat gaa gta tca ata 2348 Ile Trp Trp His Glu Val Gln Asn Glu Gly Arg Asp Glu Val Ser Ile 135 140 145 ttg caa gag aaa tat tct cta gaa cag gtg aaa aca tgt tgg ttg gag 2396 Leu Gln Glu Lys Tyr Ser Leu Glu Gln Val Lys Thr Cys Trp Leu Glu 150 155 160 cct gag gat gat tgg gag gtt gcc att agg aat tat gcc aag ata gct 2444 Pro Glu Asp Asp Trp Glu Val Ala Ile Arg Asn Tyr Ala Lys Ile Ala 165 170 175 ttg agg cct gat aaa ttg tac aga att act aaa cgg att aat att aga 2492 Leu Arg Pro Asp Lys Leu Tyr Arg Ile Thr Lys Arg Ile Asn Ile Arg 180 185 190 aat gca tgt tat ata tca ggg aat ggg gct gag gta gtg ata gac act 2540 Asn Ala Cys Tyr Ile Ser Gly Asn Gly Ala Glu Val Val Ile Asp Thr 195 200 205 210 caa gac aga aca gtt ttt aga tgc tgc atg atg ggt atg tgg cca ggg 2588 Gln Asp Arg Thr Val Phe Arg Cys Cys Met Met Gly Met Trp Pro Gly 215 220 225 gtg gtt ggc atg gag gca gta acc ctt atg aat gta aag ttt aga ggg 2636 Val Val Gly Met Glu Ala Val Thr Leu Met Asn Val Lys Phe Arg Gly 230 235 240 gat ggg tat aat ggt gtg gtt ttt atg gct aat act aaa ttg att ttg 2684 Asp Gly Tyr Asn Gly Val Val Phe Met Ala Asn Thr Lys Leu Ile Leu 245 250 255 cat ggt tgt agc ttt ttt ggt ttt aat aat ata tgt gtg gaa gct tgg 2732 His Gly Cys Ser Phe Phe Gly Phe Asn Asn Ile Cys Val Glu Ala Trp 260 265 270 ggg cag gtg agt gta aga ggc tgt agt ttc tat gca tgc tgg att gca 2780 Gly Gln Val Ser Val Arg Gly Cys Ser Phe Tyr Ala Cys Trp Ile Ala 275 280 285 290 aca tca ggc agg acc aag agt caa ttg tct gta aag aaa tgt atg ttt 2828 Thr Ser Gly Arg Thr Lys Ser Gln Leu Ser Val Lys Lys Cys Met Phe 295 300 305 gag aga tgt aac ctg ggc ata ctg aat gaa gga gaa gcc aga gtc agc 2876 Glu Arg Cys Asn Leu Gly Ile Leu Asn Glu Gly Glu Ala Arg Val Ser 310 315 320 cac tgt gct tct tcc gaa act ggc tgt ttc ata ttg ata aag gga aat 2924 His Cys Ala Ser Ser Glu Thr Gly Cys Phe Ile Leu Ile Lys Gly Asn 325 330 335 gcc aat gtg aaa cat aat atg atc tgt gga ccc tca gat gag agg cct 2972 Ala Asn Val Lys His Asn Met Ile Cys Gly Pro Ser Asp Glu Arg Pro 340 345 350 tat cag atg ctg aca tgt gct ggc gga cat tgc aat atg ctg gct acc 3020 Tyr Gln Met Leu Thr Cys Ala Gly Gly His Cys Asn Met Leu Ala Thr 355 360 365 370 gtg cat att gtt tct cac cca cgc aag aaa tgg cct gtt ttg gaa cat 3068 Val His Ile Val Ser His Pro Arg Lys Lys Trp Pro Val Leu Glu His 375 380 385 aat gtg atg acc aaa tgc act atg cac gta ggt ggt cgc aga gga atg 3116 Asn Val Met Thr Lys Cys Thr Met His Val Gly Gly Arg Arg Gly Met 390 395 400 tta atg cca tac cag tgt aac atg aat aat gtg aaa gtg atg ttg gag 3164 Leu Met Pro Tyr Gln Cys Asn Met Asn Asn Val Lys Val Met Leu Glu 405 410 415 cca gat gca ttt tcc aga atg agt tta aca gga atc ttt gac atg aat 3212 Pro Asp Ala Phe Ser Arg Met Ser Leu Thr Gly Ile Phe Asp Met Asn 420 425 430 ctg caa ata tgg aag atc ctg aga tat gat gac acg aag tcg agg gta 3260 Leu Gln Ile Trp Lys Ile Leu Arg Tyr Asp Asp Thr Lys Ser Arg Val 435 440 445 450 cgc gca tgc gag tgc ggg ggc aaa cat gcc agg ttc cag ccg gtg tgt 3308 Arg Ala Cys Glu Cys Gly Gly Lys His Ala Arg Phe Gln Pro Val Cys 455 460 465 gtg gat gtg act gaa gaa cta agg cca gat cat ttg gtg att gcc tgc 3356 Val Asp Val Thr Glu Glu Leu Arg Pro Asp His Leu Val Ile Ala Cys 470 475 480 act gga gcg gag ttc ggt tct agt ggt gaa gaa act gac taaagtgagt 3405 Thr Gly Ala Glu Phe Gly Ser Ser Gly Glu Glu Thr Asp 485 490 495 agtagtggga tggtttggat ggactctaat gtgaataaga tggacagatt gggtaaattt 3465 ttgttttttc tgtcttgcag ctgtc atg agt gga agc gct tct ttt gag ggg 3517 Met Ser Gly Ser Ala Ser Phe Glu Gly 500 gga gtc ttt agc cct tat ctg acg ggc cgt ctc cca cca tgg gca gga 3565 Gly Val Phe Ser Pro Tyr Leu Thr Gly Arg Leu Pro Pro Trp Ala Gly 505 510 515 520 gta cgt cag aat gtc atg gga tct act gtg gat ggg aga cca gtc cag 3613 Val Arg Gln Asn Val Met Gly Ser Thr Val Asp Gly Arg Pro Val Gln 525 530 535 ccc gcc aat tca tca aca ctg acc tat gcc act ttg agc tct tca ccc 3661 Pro Ala Asn Ser Ser Thr Leu Thr Tyr Ala Thr Leu Ser Ser Ser Pro 540 545 550 ttg gat gca gct gca gct gct gcc gcc tct gct gcc gcc aac acc gtc 3709 Leu Asp Ala Ala Ala Ala Ala Ala Ala Ser Ala Ala Ala Asn Thr Val 555 560 565 ctt gga att ggc tat tat gga agc atc gtt gcc aat acc agt tcc tca 3757 Leu Gly Ile Gly Tyr Tyr Gly Ser Ile Val Ala Asn Thr Ser Ser Ser 570 575 580 aat aac cct tcg acc ctg gct gag gac aag cta ctt gtt ctt ttg gcg 3805 Asn Asn Pro Ser Thr Leu Ala Glu Asp Lys Leu Leu Val Leu Leu Ala 585 590 595 600 cag ctt gag gcg ttg acc cag cgc ctg ggt gaa ctg tct cag cag gtg 3853 Gln Leu Glu Ala Leu Thr Gln Arg Leu Gly Glu Leu Ser Gln Gln Val 605 610 615 gcc cag ctg cgc gag caa act gag tct gct gtt gcc aca gca aag tct 3901 Ala Gln Leu Arg Glu Gln Thr Glu Ser Ala Val Ala Thr Ala Lys Ser 620 625 630 aaa taaagattaa tcaataaata aaggagatac ttgttgattt taaactgtaa 3954 Lys tgaatcttta tttgattttt cgcgcacggt atgccctgga ccaccggtct cgatcattga 4014 gaactcggtg gatcttttcc aggaccctgt agaggtggga ttgaatgttt agatacattg 4074 gcattaggcc gtctcgaggg tggagatagc tccattgaag agcctcgtgt tccggggtag 4134 tgttataaat cacccagtca taacaaggtc ggagtgcatg atgttgcaca atatctttaa 4194 ggagcaggct gattgcaact gggagcccct tggtgtatgt gtttacaaat ctgttgagct 4254 gagatggatg cattctgggt gaaattatat gcatttttga ctggatcttg aggttggcaa 4314 tgttgccgcc cagatcccgt ctcgggttca tgttatgcag gaccaccaag acggtgtatc 4374 cggtgcactt aggaaattta tcatgcagct tagatggaaa agcatgaaaa aatttggaga 4434 cgcctttgtg tccgcccaaa ttctccatgc actcatccat aatgatagca atggggccgt 4494 gggcggcggc acgggcaaac acgttccggg gatctgacac atcatagtta tgctcctgag 4554 acaggtcatc ataagccatt ttaataaact ttgggcgtag ggtgccagat tggggtataa 4614 atgttccctc gggccccgga gcatagtttc cctcacagat ttgcatttcc caggctttca 4674 gttcagaggg ggggatcatg tccacctgcg gggctataaa aaataccgtt tctggggctg 4734 gggtgattaa ctgtgatgat agcaaattcc ttagcagctg tgacttgcca cacccagtgg 4794 ggccgtaaat gaccccgatt acgggttgca gatggtagtt tagggagcgg cagctgccgt 4854 cctctcggag caggggggcc acttcgttca tcatttccct tacatggata ttttcccgca 4914 ccaagtccgt taggaggcgc tctccaccta gggataaaag ttcctggagg gaggagaagt 4974 ttttgagcgg cttcagcccg tcagccatgg gcattttgga gagagtctgt tgcaagagct 5034 cgagccgatc ccaaagctcg gttatgtgtt ctatggcatc tcgatccagc aaacctcctc 5094 gtttcgcgga ttggggcggc tcctggagta gggtatcaga cgatgggcgt ccagcgctgc 5154 cagtgtccga tccttccatg gtcgcagcgt ccgagtcagg gttgtttccg tcacggtgaa 5214 tgggtgcgcg cctggttgtg cgcttgcgag ggtgcgcctc aggctcatcc tgctggtcga 5274 gaaccgctgc cgatcggcgc cctgcatgtc ggccaggtag cagtttacca tgagttcgta 5334 gttgagcgct tcggccgcat ggcctttggc gcggagctta cctttggaag ttttgtgaca 5394 ggagggacag tatagacact taagggcata cagcttgggt gcgaggaaga ttgattcggg 5454 ggagtatgca tctgcgccgc aggaggcgca gacagtttcg cattccacga gccatgtcag 5514 atctggttca tctgggtcaa aaacaagttt tccgccatat tttttgatgc gtttcttacc 5574 ttttgtctcc atgagttcgt gtcctcgctg ggtgacaaag aggctgtctg tgtccccgta 5634 gaccgacttt ataggcctgt cctcgagcgg agtgcctcgg tcctcttcgt ataggaatcc 5694 cgaccactct gatacaaagg cgcgtgtcca ggctagcaca aatgaggcta cttgggaagg 5754 gtagcggtcg ttgtcaacca gggggtccac cttctctaca gtatgtaaac acatgtcccc 5814 ctcctccaca tccagaaatg tgattggctt gtaaaggtat gccacgtgac cgggagtccc 5874 agccgggggg gtataaaagg gggcgggtct ctgttcgtcc tcactgtctt ccggatcgct 5934 gtccaggagc gccaactgtt ggggtaggta ttccctctcg aaggcaggca taacctctgc 5994 actcaggttg tcagtttcta ggaacgatga ggatttgata ttgacagtgc ctgctgagat 6054 gcctttcatg agactttcgt ccatttggtc agaaaagaca atctttttgt tgtccaactt 6114 ggtagcaaag gatccatata gggcattgga taggagcttg gctatggagc gcatggtttg 6174 attcttttcc ttgtccgcgc gttccttggc ggcgatgttc agctggacat attcgcgcgc 6234 caggcacttc cattcaggga agatggttgt cagttcatcc ggcacaattc tgacttgcca 6294 gcccctatta tgtagggtta tcagatccac actggtggcc acctctcctc gaagaggttc 6354 gttggtccag cagagccgac ccccctttct cgaacagaaa gggggtagag ggtctagcat 6414 gagctcatca ggggggtctg catccatggt gaagattcct ggaagtaggt ccttgtcaaa 6474 atagctgatg ggggtgggat catctaaagc catctgccat tctcgagctg ctagcgcgcg 6534 ctcatatggg ttcagtggtg taccccaggg catgggatgg gtgagcgcag aggcatacat 6594 gccacagatg tcatagacat aaaggggctc ttctagtatg ccgatgtatg tgggataaca 6654 tcgcccccct ctgatgcttg ctcgcacata attatagagc tcatgagatg gggcaaggag 6714 acccgggccc agattagtgc ggttgggctt ctctgccctg tagacaattt ggcgaaagat 6774 ggcatgggaa ttagaagaga tagttggcct ttggaatatg ttaaagtggg catggggtaa 6834 acctacagaa tccctgatga agtgggcata tgattcttgc aacttggcca ctagctctgc 6894 ggtgaccagg acgtccatgg cgcagtagtc gagggtctct ttgatgatgt cataacctgg 6954 ttggtttttt ttttcccaca gctcgcggtt gaggaggtat tcttcgcgat ccttccagta 7014 ctcttcgagg ggaaacccgt ctttgtctgc acggtaagag cccagcatgt agaattgatt 7074 gactgccttg taaggacagc accccttctc cacagggaga gagtatgctt gagcggcttt 7134 gcgcagtgag gtatgagtaa gggcgaaggt gtccctgacc ataactttga ggaactggta 7194 cttgaagtcg atgtcgtcac acgtcccctg ttcccagagt tggaagtcca cccgcttctt 7254 gtaggcgggg ttgggcaaag cgaaagtaac atcgttgaag agaatcttgc cggccctggg 7314 caaaaaattg cgggtaatgc ggaaaggctg gggcacctct gctcgattat tgatcacttg 7374 cgcagctagg acgatctcgt caaagccgtt aatgttgtgc cccactatgt acatttctat 7434 gaatcgtggg gagcctctga tgtgaggtag ctttttgagc tcttcgaagg tgaggtctgt 7494 agggtcagag agagcgtagt gttcgagggc ccattcgtgc aggtgagggt ttgcattcat 7554 gaaagatgac caaagatcca ctgccagtgc tgtttgtaac tggtcccggt actggcgaaa 7614 atgctgaccg actgccatct tttctggggt gacacagtag aatgttttgg ggtcctgctg 7674 ccaacgatcc cacttgagtt tcatggcgag atcgtaggcg atgttgacga gccgttcgtc 7734 ccccgaaagt ttcatgacca gcatgaaggg gactagctgc tttccaaagg accccatcca 7794 ggtgtaggtt tccacatcgt aggtgaggaa gagcctttct gtgcgaggat gagagccaat 7854 cgggaagaac tggatctcct gccaccagtt ggaggaatgg ctgttgatgt gatggaagta 7914 gaactccctt cggcgcgccg agcattcatg cttgtgcttg tacagacggc cgcagtactc 7974 gcagcgctgc acgggatgca cctcatgaat gagttgtacc tggtttcctt tgacaagaaa 8034 tttcagtggg aagttgaggc ctggcgtctg tacctcgtgc tctactatgt tatttgcatc 8094 ggcctggcca tcttctgtct cgatggtggt catgctgacg agaccccgcg ggaggcaagt 8154 ccagatctcg gcgcgggagg ggcggagctc gaggacgaga gcgcgcaggc cggagctgtc 8214 cagggtcctg agtcgctgcg gagtcaggtt agtagggagg ctctggagat tgacttgcaa 8274 gattttttcg agggcatggg ggaggttaag atggtacttg atctctactg gtccgttggt 8334 ggagatgtcg atggcttgca gggttccatg tcccttgggc gccaccactg tgcccttgtt 8394 tttccttttt ggcgggagtg gtggtggctc tgttgcttct tgcatgttca gaatcggtgg 8454 cgagggcgag cgccgggcgg taggggcggc tcgggccccg gtggcatggc cggcagtggc 8514 acgtcggcgc cgcgtgcggg taggttctgg tactgcgccc tgagaagact tgcgtgcgca 8574 acgacgcggc ggttgacgtc ttggatctgc cgcctctggg tgaaagctac cggacccgtg 8634 agcttgaacc tgaaagagag ttcaacagaa tcaatttcgg tatcgttaac ggcggcctgt 8694 ctcaggatct cttgcacgtc gcctgagttg tcctggtagg cgatctcggc catgaattgc 8754 tcgatttctt cctcctgaag atctccgcga cccgctctct cgacggtggc cgcgaggtcg 8814 ttggaaatgc gggccatgag ttgagagaat gcattcatgc ccgcctcgtt ccagacgcgg 8874 ctgtagacca cggccccttc gggatctctt gcgcgcatga ccacctgggc aaggttgagc 8934 tccacgtggc gcgtgaagac cgcatagttg cagaggcgct ggtataggta gttgagtgtg 8994 gtggcgatat gctcggtgac gaagaagtac atgatccatc gtctcagcgg catttcgctg 9054 acatcgccca gggcttccaa gcgctccatg gcctcgtaga agtccacggc gaagttgaaa 9114 aactgagagt ttcgcgcgga cacggtcaac tcctcctcca gaagacggat gagttcggcg 9174 atggtggcgc gcacttcgcg ctcgaaggcc cccgggattt cttcctcctc ttctaactct 9234 tcttccacta acatctcttc ttcctcttca ggcgggggcg gaggaggagg agggggtacg 9294 cggcgacgcc ggcggcgcac gggcaaacgg tcgatgaatc tttcaatgac ctctccgcgg 9354 cggcggcgca tggtctcggt gacggcacgg ccgttctccc tgggtctcaa agtgaaaacg 9414 cctccgcgca tctccctgaa gtggtgactt gggggctctc cgttgggcag tgaaagggcg 9474 ctgattatgc actttatcaa ttgtcctgta gggactccgc gcaaggacct gatcgtctca 9534 agatccacgg gatctgaaaa tctttcaacg aaagcgtcta accagtcgca atcgcaaggt 9594 aggctgagca ctgtttcttg ctggcggggg tggctacacg ctgggtcggg gttctctctt 9654 tcttctcctt cctcctcttg ggagggtgag acgatgctgc tggtgatgaa attaaaatag 9714 gcagttctga gacggcggat ggtggcgagg agcaccaggt ctttgggacc ggcttgctgg 9774 atgcgcaggc gattggccat tccccaagca ttatcctgac acctggccag atttttgtag 9834 tagtcttgca taagtcgctc cacgggcact tcttcttcgc ccgctctgcc atgcatgcgc 9894 gtgagcccaa acccacgcat gggctggaca agtgccaggt ctgctacgac cctttctgcg 9954 aggatggctt gctgcacctg agtgagggtg gcttggaaat cgtcgaagtc cacaaaacga 10014 tggtaggccc cggtgttgat ggtgtaagag cagttggcca tgactgacca gttaactgtc 10074 tggtgccccg ggcgcacaag ctcggtgtac ttgaggcgcg agtaggcgcg ggtgtcaaag 10134 atgtaatcgt tacaggtgcg caccaggtac tggtagccga tgagaaagtg cggcggcggc 10194 tggcggtata ggggccatcg ctctgtagcc ggggcgccag gggcgaggtc ttccagcatg 10254 aggcggtgat aaccgtagat gtacctggac atccaggtga taccggaggc ggtggtggat 10314 gcccgcggga actcgcgtac gcggttccag atgttgcgca gcggcatgaa gtagttcatg 10374 gtaggcacgg tttggcccgt gaggcgcgca cagtcgttga tgctctagac atacgggcaa 10434 aaacgaaagc ggtcagcggc tcgtctccgt ggcctggagg ctaagcgaac gggttgggct 10494 gcgcgtgtac cccggttcga atctcggatc aggctggagc cgcagctaac gtggtactgg 10554 cactcccgtc tcgacccagg cctgcacaaa acctccagga tacggaggcg ggtcgttttt 10614 tttttttttt gctttttcct ggattggagc cagtgctgcg tcaagcttta gaacgctcag 10674 ttcgcggggt tgggagtggc tcgcgcccgt agtctggaga atcaatcgcc agggttgcgt 10734 tgcggtgtgc cccggttcga gtcttagcgc gccggatcgg ccggtttccg cgacaagcga 10794 gggtttggca gccccgtcat ttctaagacc ccgccagccg acttctccag tttacgggag 10854 cgagccctct tttttttttt ttgttgccca g atg cat ccc gtg ctg cga cag 10906 Met His Pro Val Leu Arg Gln 635 640 atg cgc ccc cag caa cag ccc cct tct cag cag cag cta cag caa cag 10954 Met Arg Pro Gln Gln Gln Pro Pro Ser Gln Gln Gln Leu Gln Gln Gln 645 650 655 cca caa aag gct ctt cct gct cct gta act act gcg gct gca gcc gtc 11002 Pro Gln Lys Ala Leu Pro Ala Pro Val Thr Thr Ala Ala Ala Ala Val 660 665 670 agc ggc gcg gga cag ccc gcc tat gat ctg gac ttg gaa gag ggc gag 11050 Ser Gly Ala Gly Gln Pro Ala Tyr Asp Leu Asp Leu Glu Glu Gly Glu 675 680 685 gga ctg gcg cgc ctg ggt gca cca tcg ccc gag cgg cac ccg cgg gtg 11098 Gly Leu Ala Arg Leu Gly Ala Pro Ser Pro Glu Arg His Pro Arg Val 690 695 700 caa ctg aaa aag gac tct cgc gag gcg tac gtg ccc cag cag aac ctg 11146 Gln Leu Lys Lys Asp Ser Arg Glu Ala Tyr Val Pro Gln Gln Asn Leu 705 710 715 720 ttc agg gac agg agc ggc gag gag cct gag gaa atg cga gct tcc cgc 11194 Phe Arg Asp Arg Ser Gly Glu Glu Pro Glu Glu Met Arg Ala Ser Arg 725 730 735 ttt aac gcg ggt cgc gaa ctg cgt cac ggt ctg gac cga aga cgg gtg 11242 Phe Asn Ala Gly Arg Glu Leu Arg His Gly Leu Asp Arg Arg Arg Val 740 745 750 ctg cgt gat gat gat ttt gaa gtc gat gaa gtg aca gga ata agt cct 11290 Leu Arg Asp Asp Asp Phe Glu Val Asp Glu Val Thr Gly Ile Ser Pro 755 760 765 gct agg gca cat gtg gcc gcg gcc aac cta gta tca gct tac gag cag 11338 Ala Arg Ala His Val Ala Ala Ala Asn Leu Val Ser Ala Tyr Glu Gln 770 775 780 acc gtg aag gag gag cgc aac ttt caa aaa tct ttc aac aac cat gtg 11386 Thr Val Lys Glu Glu Arg Asn Phe Gln Lys Ser Phe Asn Asn His Val 785 790 795 800 cgc acc ctg att gcc cgc gag gaa gtg aca ctg ggt ctg atg cac ctg 11434 Arg Thr Leu Ile Ala Arg Glu Glu Val Thr Leu Gly Leu Met His Leu 805 810 815 tgg gac ctg atg gaa gct att acc cag aac ccc acc agc aaa cct ctg 11482 Trp Asp Leu Met Glu Ala Ile Thr Gln Asn Pro Thr Ser Lys Pro Leu 820 825 830 acc gct cag ctg ttt ctg gtg gtg caa cat agt aga gac aat gag gca 11530 Thr Ala Gln Leu Phe Leu Val Val Gln His Ser Arg Asp Asn Glu Ala 835 840 845 ttt agg gag gcg ctg ttg aac att act gag ccc gag ggg aga tgg ttg 11578 Phe Arg Glu Ala Leu Leu Asn Ile Thr Glu Pro Glu Gly Arg Trp Leu 850 855 860 tat gat ctt atc aat att ctg caa agt ata ata gtg caa gaa cgt agc 11626 Tyr Asp Leu Ile Asn Ile Leu Gln Ser Ile Ile Val Gln Glu Arg Ser 865 870 875 880 ctg ggt cta gct gag aag gtg gct gct att aac tac tcg gtc ttg agc 11674 Leu Gly Leu Ala Glu Lys Val Ala Ala Ile Asn Tyr Ser Val Leu Ser 885 890 895 ctg ggc aag cac tac gct cgc aag atc tac aaa acc cca tac gta cct 11722 Leu Gly Lys His Tyr Ala Arg Lys Ile Tyr Lys Thr Pro Tyr Val Pro 900 905 910 ata gac aag gag gtg aag ata gat ggg ttt tat atg cgc atg act ctc 11770 Ile Asp Lys Glu Val Lys Ile Asp Gly Phe Tyr Met Arg Met Thr Leu 915 920 925 aag gtg ctg acc tta agt gac gat ctg gga gtg tac cgc aac gac agg 11818 Lys Val Leu Thr Leu Ser Asp Asp Leu Gly Val Tyr Arg Asn Asp Arg 930 935 940 atg cac cgc gca gtg agc gcc agc aga agg cgt gag ctg agc gac aga 11866 Met His Arg Ala Val Ser Ala Ser Arg Arg Arg Glu Leu Ser Asp Arg 945 950 955 960 gaa ctt atg cac agc ttg caa aga gct ctg acg ggg gct gga acc gag 11914 Glu Leu Met His Ser Leu Gln Arg Ala Leu Thr Gly Ala Gly Thr Glu 965 970 975 ggg gag acc tac ttt gac atg gga gcg gac ttg cag tgg cag ccc agt 11962 Gly Glu Thr Tyr Phe Asp Met Gly Ala Asp Leu Gln Trp Gln Pro Ser 980 985 990 cgc agg gcc ctg gac gca gca ggg tat gag ctt cct tac ata gaa gag 12010 Arg Arg Ala Leu Asp Ala Ala Gly Tyr Glu Leu Pro Tyr Ile Glu Glu 995 1000 1005 gtg gat gca ggc cag gat gag gag ggc gag tac ctg gaa gac 12052 Val Asp Ala Gly Gln Asp Glu Glu Gly Glu Tyr Leu Glu Asp 1010 1015 1020 tgatggcgcg accatccata tttttgctag atg gaa cag cag gca ccg gac 12103 Met Glu Gln Gln Ala Pro Asp 1025 ccc gca ata cgg gcg gcg cta cag agc cag ccg tcc ggc att aac 12148 Pro Ala Ile Arg Ala Ala Leu Gln Ser Gln Pro Ser Gly Ile Asn 1030 1035 1040 tcc tcg gac gat tgg agc cag gcc atg caa cgc atc atg gcg ctg 12193 Ser Ser Asp Asp Trp Ser Gln Ala Met Gln Arg Ile Met Ala Leu 1045 1050 1055 acg acc cgc aac ccc gaa gcc ttt aga cag caa ccc cag gcc aac 12238 Thr Thr Arg Asn Pro Glu Ala Phe Arg Gln Gln Pro Gln Ala Asn 1060 1065 1070 cgc ctt tct gcc atc ctg gag gcc gta gtg ccc tcc cgc tcc aac 12283 Arg Leu Ser Ala Ile Leu Glu Ala Val Val Pro Ser Arg Ser Asn 1075 1080 1085 ccc aca cac gag aag gtc ctg gcc atc gtg aac gcg ctg gtg gag 12328 Pro Thr His Glu Lys Val Leu Ala Ile Val Asn Ala Leu Val Glu 1090 1095 1100 aac aaa gcc ata cgt ccc gat gag gct ggg ctg gta tac aat gcc 12373 Asn Lys Ala Ile Arg Pro Asp Glu Ala Gly Leu Val Tyr Asn Ala 1105 1110 1115 cta ttg gag cgc gta gcc cgt tac aac agc agc aac gtg cag acc 12418 Leu Leu Glu Arg Val Ala Arg Tyr Asn Ser Ser Asn Val Gln Thr 1120 1125 1130 aac ctg gac cgg atg gtg acc gat gtg cgc gag gcc gtg tcc cag 12463 Asn Leu Asp Arg Met Val Thr Asp Val Arg Glu Ala Val Ser Gln 1135 1140 1145 cgc gag cgg ttc cag cga gac gcc aat tta ggg tcg ctg gtg gct 12508 Arg Glu Arg Phe Gln Arg Asp Ala Asn Leu Gly Ser Leu Val Ala 1150 1155 1160 ttg aac gcc ttc ctc agc act cag cct gcc aac gtg cct cgc ggt 12553 Leu Asn Ala Phe Leu Ser Thr Gln Pro Ala Asn Val Pro Arg Gly 1165 1170 1175 cag caa gac tac aca aac ttt cta agt gca tta aga ctc atg gtg 12598 Gln Gln Asp Tyr Thr Asn Phe Leu Ser Ala Leu Arg Leu Met Val 1180 1185 1190 gcc gaa gtc cct caa agc gaa gtg tac cag tcc ggg cca gac tac 12643 Ala Glu Val Pro Gln Ser Glu Val Tyr Gln Ser Gly Pro Asp Tyr 1195 1200 1205 ttt ttc cag acc agc aga cag ggc ttg cag aca gtg aac ctg agt 12688 Phe Phe Gln Thr Ser Arg Gln Gly Leu Gln Thr Val Asn Leu Ser 1210 1215 1220 cag gct ttt aag aac ctg aat ggt ctg tgg gga gtg cgc gcc cca 12733 Gln Ala Phe Lys Asn Leu Asn Gly Leu Trp Gly Val Arg Ala Pro 1225 1230 1235 gtg gga gat cgg gcg acc gtg tct agc ttg ctg acc ccc aac tcc 12778 Val Gly Asp Arg Ala Thr Val Ser Ser Leu Leu Thr Pro Asn Ser 1240 1245 1250 cgc cta cta ctt ctc ttg gta gcc cca ttc act gac agc ggt agc 12823 Arg Leu Leu Leu Leu Leu Val Ala Pro Phe Thr Asp Ser Gly Ser 1255 1260 1265 atc gac cgt aat tct tac ttg ggc tat ctg ttg aac ctg tat cgc 12868 Ile Asp Arg Asn Ser Tyr Leu Gly Tyr Leu Leu Asn Leu Tyr Arg 1270 1275 1280 gag gcc ata ggg caa act cag gta gat gag caa acc tat caa gaa 12913 Glu Ala Ile Gly Gln Thr Gln Val Asp Glu Gln Thr Tyr Gln Glu 1285 1290 1295 att acc caa gtg agc cgc gct ctg ggt cag gag gac act ggc agc 12958 Ile Thr Gln Val Ser Arg Ala Leu Gly Gln Glu Asp Thr Gly Ser 1300 1305 1310 ttg gaa gcc acc tta aac ttc ttg ctg acc aac cgg tcg cag aag 13003 Leu Glu Ala Thr Leu Asn Phe Leu Leu Thr Asn Arg Ser Gln Lys 1315 1320 1325 atc cct cct cag tat gcg ctt acc gcg gag gag gaa cgg atc ctg 13048 Ile Pro Pro Gln Tyr Ala Leu Thr Ala Glu Glu Glu Arg Ile Leu 1330 1335 1340 aga tac gtg cag cag agc gtg gga ctg ttc cta atg cag gag ggg 13093 Arg Tyr Val Gln Gln Ser Val Gly Leu Phe Leu Met Gln Glu Gly 1345 1350 1355 gcg act cct act gct gcg ctc gat atg aca gcc cga aac atg gag 13138 Ala Thr Pro Thr Ala Ala Leu Asp Met Thr Ala Arg Asn Met Glu 1360 1365 1370 ccc agc atg tat gcc agt aac cgg cct ttt atc aat aaa ctg cta 13183 Pro Ser Met Tyr Ala Ser Asn Arg Pro Phe Ile Asn Lys Leu Leu 1375 1380 1385 gac tac tta cac agg gcg gct gct atg aac tct gat tat ttc acc 13228 Asp Tyr Leu His Arg Ala Ala Ala Met Asn Ser Asp Tyr Phe Thr 1390 1395 1400 aat gct atc ctg aac ccc cat tgg ctg ccc cca cct ggg ttc tat 13273 Asn Ala Ile Leu Asn Pro His Trp Leu Pro Pro Pro Gly Phe Tyr 1405 1410 1415 acg ggc gag tat gac atg ccc gac ccc aat gac ggg ttt tta tgg 13318 Thr Gly Glu Tyr Asp Met Pro Asp Pro Asn Asp Gly Phe Leu Trp 1420 1425 1430 gac gat gtg gac agt agt gtt ttc tcc ccg cct cct ggt tat aac 13363 Asp Asp Val Asp Ser Ser Val Phe Ser Pro Pro Pro Gly Tyr Asn 1435 1440 1445 act tgg aag aag gaa ggt ggc gat aga agg cac tct tcc gtg tca 13408 Thr Trp Lys Lys Glu Gly Gly Asp Arg Arg His Ser Ser Val Ser 1450 1455 1460 ctg tcc ggg gca acg ggt gct gcc gca gcg gtt ccc gag gct gca 13453 Leu Ser Gly Ala Thr Gly Ala Ala Ala Ala Val Pro Glu Ala Ala 1465 1470 1475 agt cct ttc cct agt ttg cca ttt tcg cta aac agt gta cgc agc 13498 Ser Pro Phe Pro Ser Leu Pro Phe Ser Leu Asn Ser Val Arg Ser 1480 1485 1490 agt gag ctg gga aga ata acc cgt cct cgc ttg atc ggc gag gag 13543 Ser Glu Leu Gly Arg Ile Thr Arg Pro Arg Leu Ile Gly Glu Glu 1495 1500 1505 gag tat ttg aac gac tcc ctg ttg aga ccc gag agg gag aag aat 13588 Glu Tyr Leu Asn Asp Ser Leu Leu Arg Pro Glu Arg Glu Lys Asn 1510 1515 1520 ttc ccc aac aac ggg ata gaa agc ttg gtt gac aaa atg aac cgc 13633 Phe Pro Asn Asn Gly Ile Glu Ser Leu Val Asp Lys Met Asn Arg 1525 1530 1535 tgg aag acg tac gcg cac gat cac agg gac gat ccc cgg gcg ctg 13678 Trp Lys Thr Tyr Ala His Asp His Arg Asp Asp Pro Arg Ala Leu 1540 1545 1550 ggg gat agc cgg ggc agc gct acc cgt aaa cgc cag tgg cac gac 13723 Gly Asp Ser Arg Gly Ser Ala Thr Arg Lys Arg Gln Trp His Asp 1555 1560 1565 agg cag cgg ggc ctg gtg tgg gcc gat gag gat tcc gcc gac gac 13768 Arg Gln Arg Gly Leu Val Trp Ala Asp Glu Asp Ser Ala Asp Asp 1570 1575 1580 agc agc gtg ttg gac ttg ggt ggg agt ggt ggt aac ccg ttc gct 13813 Ser Ser Val Leu Asp Leu Gly Gly Ser Gly Gly Asn Pro Phe Ala 1585 1590 1595 cac ctg cgc ccc cgc gtc ggg cgc ctg atg taagaaaccg aaaataaata 13863 His Leu Arg Pro Arg Val Gly Arg Leu Met 1600 1605 ctcaccaagg ccatggcgac cagcgtgcgt tcgtttcttc tctgttatat ctagt atg 13921 Met 1610 atg agg cga acc gtg cta ggc gga gcg gtg gtg tat ccg gag ggt 13966 Met Arg Arg Thr Val Leu Gly Gly Ala Val Val Tyr Pro Glu Gly 1615 1620 1625 cct cct cct tcg tac gag agc gtg atg cag cag gcg gcg gcg gcg 14011 Pro Pro Pro Ser Tyr Glu Ser Val Met Gln Gln Ala Ala Ala Ala 1630 1635 1640 gcg atg cag cca cca ctg gag gct ccc ttt gta ccc cct cgg tac 14056 Ala Met Gln Pro Pro Leu Glu Ala Pro Phe Val Pro Pro Arg Tyr 1645 1650 1655 ctg gca cct acg gag ggg aga aac agc att cgt tac tcg gag ctg 14101 Leu Ala Pro Thr Glu Gly Arg Asn Ser Ile Arg Tyr Ser Glu Leu 1660 1665 1670 gca cca ttg tat gat acc acc cgg ttg tat ttg gtg gac aac aag 14146 Ala Pro Leu Tyr Asp Thr Thr Arg Leu Tyr Leu Val Asp Asn Lys 1675 1680 1685 tcc gcg gac atc gcc tca ctg aac tat cag aac gac cac agc aac 14191 Ser Ala Asp Ile Ala Ser Leu Asn Tyr Gln Asn Asp His Ser Asn 1690 1695 1700 ttc ctc acc acg gtg gtg caa aac aat gac ttt acc ccc acg gag 14236 Phe Leu Thr Thr Val Val Gln Asn Asn Asp Phe Thr Pro Thr Glu 1705 1710 1715 gcc agc acc cag acc atc aac ttt gac gag cgg tcg cga tgg ggc 14281 Ala Ser Thr Gln Thr Ile Asn Phe Asp Glu Arg Ser Arg Trp Gly 1720 1725 1730 ggt cag ctg aag act atc atg cac acc aac atg ccc aac gtg aac 14326 Gly Gln Leu Lys Thr Ile Met His Thr Asn Met Pro Asn Val Asn 1735 1740 1745 gag tac atg ttt agc aac aag ttc aaa gct cgg gtg atg gtg tct 14371 Glu Tyr Met Phe Ser Asn Lys Phe Lys Ala Arg Val Met Val Ser 1750 1755 1760 aga aag gct cct gaa ggt gtc aca gta gat gac aat tat gat cac 14416 Arg Lys Ala Pro Glu Gly Val Thr Val Asp Asp Asn Tyr Asp His 1765 1770 1775 aag cag gat att ttg gaa tat gag tgg ttt gag ttt act cta ccg 14461 Lys Gln Asp Ile Leu Glu Tyr Glu Trp Phe Glu Phe Thr Leu Pro 1780 1785 1790 gaa ggg aac ttc tca gcc aca atg acc att gac cta atg aac aat 14506 Glu Gly Asn Phe Ser Ala Thr Met Thr Ile Asp Leu Met Asn Asn 1795 1800 1805 gcc atc att gat aat tac ctt gaa gtg ggc aga cag aat gga gtg 14551 Ala Ile Ile Asp Asn Tyr Leu Glu Val Gly Arg Gln Asn Gly Val 1810 1815 1820 ttg gag agt gac att ggt gtt aaa ttt gac acc agg aac ttt aga 14596 Leu Glu Ser Asp Ile Gly Val Lys Phe Asp Thr Arg Asn Phe Arg 1825 1830 1835 ctg ggt tgg gat ccg gaa act aag ttg att atg cct ggg gtt tac 14641 Leu Gly Trp Asp Pro Glu Thr Lys Leu Ile Met Pro Gly Val Tyr 1840 1845 1850 acc tat gag gca ttc cat cct gac att gta ttg ttg cct ggt tgc 14686 Thr Tyr Glu Ala Phe His Pro Asp Ile Val Leu Leu Pro Gly Cys 1855 1860 1865 gga gtt gac ttt act gaa agt cgc ctt agt aac ttg ctt ggt atc 14731 Gly Val Asp Phe Thr Glu Ser Arg Leu Ser Asn Leu Leu Gly Ile 1870 1875 1880 agg aaa aga cac cca ttc cag gag ggt ttt aag atc ttg tat gag 14776 Arg Lys Arg His Pro Phe Gln Glu Gly Phe Lys Ile Leu Tyr Glu 1885 1890 1895 gat ctt gaa ggg ggt aat atc ccg gcc ctg ttg gat gta gaa gcc 14821 Asp Leu Glu Gly Gly Asn Ile Pro Ala Leu Leu Asp Val Glu Ala 1900 1905 1910 tat gag aac agt aag aaa gaa caa gaa gcc aaa aca gaa gcc gct 14866 Tyr Glu Asn Ser Lys Lys Glu Gln Glu Ala Lys Thr Glu Ala Ala 1915 1920 1925 aaa gct gct gct att gct aaa gcc aac ata gtt gtc agc gac cct 14911 Lys Ala Ala Ala Ile Ala Lys Ala Asn Ile Val Val Ser Asp Pro 1930 1935 1940 gta agg gtg gct aat gcc gaa gaa gtc aga gga gac aac tat aca 14956 Val Arg Val Ala Asn Ala Glu Glu Val Arg Gly Asp Asn Tyr Thr 1945 1950 1955 gct tca tct gtt gca act gaa gaa tcg cta ttg gct gct gtg gcc 15001 Ala Ser Ser Val Ala Thr Glu Glu Ser Leu Leu Ala Ala Val Ala 1960 1965 1970 gaa acc gaa act aca gag aca aaa ctc act att aaa cct gta gaa 15046 Glu Thr Glu Thr Thr Glu Thr Lys Leu Thr Ile Lys Pro Val Glu 1975 1980 1985 aaa gac agc aag agt aga agt tac aat gtc ttg gaa gat aaa gtc 15091 Lys Asp Ser Lys Ser Arg Ser Tyr Asn Val Leu Glu Asp Lys Val 1990 1995 2000 aat aca gcc tac cgc agc tgg tac ctg tcc tac aac tat ggt gac 15136 Asn Thr Ala Tyr Arg Ser Trp Tyr Leu Ser Tyr Asn Tyr Gly Asp 2005 2010 2015 cct gaa aaa gga gtc cgt tcc tgg aca ctg ctc acc acc tcg gat 15181 Pro Glu Lys Gly Val Arg Ser Trp Thr Leu Leu Thr Thr Ser Asp 2020 2025 2030 gtc acc tgt gga gca gag cag gtg tac tgg tcg ctc cca gac atg 15226 Val Thr Cys Gly Ala Glu Gln Val Tyr Trp Ser Leu Pro Asp Met 2035 2040 2045 atg cag gac cct gtc aca ttc cgt tcc acg aga caa gtc agc aac 15271 Met Gln Asp Pro Val Thr Phe Arg Ser Thr Arg Gln Val Ser Asn 2050 2055 2060 tat cca gtg gta ggt gca gag ctc atg ccg gtc ttc tca aag agt 15316 Tyr Pro Val Val Gly Ala Glu Leu Met Pro Val Phe Ser Lys Ser 2065 2070 2075 ttc tac aac gag caa gcc gtg tac tcc cag cag ctt cgc cag tcc 15361 Phe Tyr Asn Glu Gln Ala Val Tyr Ser Gln Gln Leu Arg Gln Ser 2080 2085 2090 acc tcg ctc acg cac gtc ttc aac cgc ttc cct gag aac cag atc 15406 Thr Ser Leu Thr His Val Phe Asn Arg Phe Pro Glu Asn Gln Ile 2095 2100 2105 ctc atc cgc ccg cca gcg ccc acc att acc acc gtc agt gaa aac 15451 Leu Ile Arg Pro Pro Ala Pro Thr Ile Thr Thr Val Ser Glu Asn 2110 2115 2120 gtt cct gct ctc aca gat cac ggg acc ctg ccg ttg cgc agc agt 15496 Val Pro Ala Leu Thr Asp His Gly Thr Leu Pro Leu Arg Ser Ser 2125 2130 2135 atc cgg gga gtc cag cgc gtg acc gtt act gac gcc aga cgc cgc 15541 Ile Arg Gly Val Gln Arg Val Thr Val Thr Asp Ala Arg Arg Arg 2140 2145 2150 acc tgc ccc tac gtc tac aag gcc ctg ggc ata gtc gcg ccg cgc 15586 Thr Cys Pro Tyr Val Tyr Lys Ala Leu Gly Ile Val Ala Pro Arg 2155 2160 2165 gtc ctt tca agc cgc act ttc taaaaaaaaa aaa atg tcc att ctt atc 15635 Val Leu Ser Ser Arg Thr Phe Met Ser Ile Leu Ile 2170 2175 tca cct agt aat aac acc ggt tgg ggc ctg cgc gcg cca agc aag 15680 Ser Pro Ser Asn Asn Thr Gly Trp Gly Leu Arg Ala Pro Ser Lys 2180 2185 2190 atg tac gga ggt gct cgc aaa cgc tct aca cag cac cct gtg cgc 15725 Met Tyr Gly Gly Ala Arg Lys Arg Ser Thr Gln His Pro Val Arg 2195 2200 2205 gtg cgc ggg cac ttc cgc gct cca tgg ggc gcc ctc aag ggt cgt 15770 Val Arg Gly His Phe Arg Ala Pro Trp Gly Ala Leu Lys Gly Arg 2210 2215 2220 acc cgc act aga acc acc gtc gat gat gtg atc gac cag gtg gtg 15815 Thr Arg Thr Arg Thr Thr Val Asp Asp Val Ile Asp Gln Val Val 2225 2230 2235 gcc gat gct cgt aat tat act cct act gca cct aca tct act gtg 15860 Ala Asp Ala Arg Asn Tyr Thr Pro Thr Ala Pro Thr Ser Thr Val 2240 2245 2250 gat gca gtt att gac agc gta gtg gct gac gcc cgc gcc tat gct 15905 Asp Ala Val Ile Asp Ser Val Val Ala Asp Ala Arg Ala Tyr Ala 2255 2260 2265 cgc cgg aag agc agg cgg aga cgc atc gcc agg cgc cac cgg gct 15950 Arg Arg Lys Ser Arg Arg Arg Arg Ile Ala Arg Arg His Arg Ala 2270 2275 2280 act ccc gct atg cga gcg gca aga gct ctg cta cgg agg gcc aaa 15995 Thr Pro Ala Met Arg Ala Ala Arg Ala Leu Leu Arg Arg Ala Lys 2285 2290 2295 cgc gtg ggg cga aga gct atg ctt aga gcg gcc aga cgc gcg gct 16040 Arg Val Gly Arg Arg Ala Met Leu Arg Ala Ala Arg Arg Ala Ala 2300 2305 2310 tca ggt gcc agt gcc ggc agg tcc cgc agg cgc gca gcc acg gcg 16085 Ser Gly Ala Ser Ala Gly Arg Ser Arg Arg Arg Ala Ala Thr Ala 2315 2320 2325 gca gca gcg gcc att gcc aac atg gcc caa ccg cga aga ggc aat 16130 Ala Ala Ala Ala Ile Ala Asn Met Ala Gln Pro Arg Arg Gly Asn 2330 2335 2340 gtg tac tgg gtg cgc gac gcc acc acc ggc cag cgc gtg ccc gtg 16175 Val Tyr Trp Val Arg Asp Ala Thr Thr Gly Gln Arg Val Pro Val 2345 2350 2355 cgc acc cgc ccc cct cgc tct tagaagatac tgagcagtct ccgatgttgt 16226 Arg Thr Arg Pro Pro Arg Ser 2360 gtcccagcga gg atg tcc aag cgc aaa tac aag gaa gag atg ctc cag 16274 Met Ser Lys Arg Lys Tyr Lys Glu Glu Met Leu Gln 2365 2370 2375 gtc atc gcg cct gaa atc tac ggt ccg ccg gtg aag gat gaa aaa 16319 Val Ile Ala Pro Glu Ile Tyr Gly Pro Pro Val Lys Asp Glu Lys 2380 2385 2390 aag ccc cgc aaa atc aag cgg gtc aaa aag gac aaa aag gaa gaa 16364 Lys Pro Arg Lys Ile Lys Arg Val Lys Lys Asp Lys Lys Glu Glu 2395 2400 2405 gat ggc aat gat ggt ctg gtg gag ttt gta cgc gag ttc gcc cca 16409 Asp Gly Asn Asp Gly Leu Val Glu Phe Val Arg Glu Phe Ala Pro 2410 2415 2420 agg cgg cgt gtg cag tgg cgt gga cgc aaa gtg cgg cct gtg ctg 16454 Arg Arg Arg Val Gln Trp Arg Gly Arg Lys Val Arg Pro Val Leu 2425 2430 2435 aga cct gga acc acg gtg gtc ttt acg ccc ggc gag cgc acc agc 16499 Arg Pro Gly Thr Thr Val Val Phe Thr Pro Gly Glu Arg Thr Ser 2440 2445 2450 act gct ttt aag cga tcc tat gat gag gtg tat ggg gat gat gat 16544 Thr Ala Phe Lys Arg Ser Tyr Asp Glu Val Tyr Gly Asp Asp Asp 2455 2460 2465 att ctg gag cag gcg gcc gac cgc ctg ggc gag ttt gct tat ggc 16589 Ile Leu Glu Gln Ala Ala Asp Arg Leu Gly Glu Phe Ala Tyr Gly 2470 2475 2480 aag cgc tcc cgc tcc agc ccc aag gag gag gcg gtg tcc att ccc 16634 Lys Arg Ser Arg Ser Ser Pro Lys Glu Glu Ala Val Ser Ile Pro 2485 2490 2495 ttg gac aat ggg aat ccc acc cct agc ctc aag cca gtc acc ctg 16679 Leu Asp Asn Gly Asn Pro Thr Pro Ser Leu Lys Pro Val Thr Leu 2500 2505 2510 cag caa gtg ctg ccc gtg cct cca cgc aga ggc aac aag cga gag 16724 Gln Gln Val Leu Pro Val Pro Pro Arg Arg Gly Asn Lys Arg Glu 2515 2520 2525 ggt gag gat ctg tat ccc acg atg caa ttg atg gtg ccc aag cgc 16769 Gly Glu Asp Leu Tyr Pro Thr Met Gln Leu Met Val Pro Lys Arg 2530 2535 2540 cag cgg ctg gag gac gtg ctg gag aaa atg aaa gtg gat ccc gat 16814 Gln Arg Leu Glu Asp Val Leu Glu Lys Met Lys Val Asp Pro Asp 2545 2550 2555 ata caa cct gag gtc aaa gtg aga ccc atc aag cag gtg gcg cca 16859 Ile Gln Pro Glu Val Lys Val Arg Pro Ile Lys Gln Val Ala Pro 2560 2565 2570 ggt ttg gga gta caa acc gta gac atc aag att ccc acc gag tcc 16904 Gly Leu Gly Val Gln Thr Val Asp Ile Lys Ile Pro Thr Glu Ser 2575 2580 2585 atg gaa gtc caa acc gaa cct gca aag ccc aca acc acc tcc att 16949 Met Glu Val Gln Thr Glu Pro Ala Lys Pro Thr Thr Thr Ser Ile 2590 2595 2600 gag gtg caa acg gat ccc tgg atg tcc gca ccc gtt aca gct caa 16994 Glu Val Gln Thr Asp Pro Trp Met Ser Ala Pro Val Thr Ala Gln 2605 2610 2615 gct gct gtc aac acc act cga aga tcc cgg cga aag tac ggt cca 17039 Ala Ala Val Asn Thr Thr Arg Arg Ser Arg Arg Lys Tyr Gly Pro 2620 2625 2630 gca agt ttg ctg atg cca aat tat gct ctg cac cca tct att att 17084 Ala Ser Leu Leu Met Pro Asn Tyr Ala Leu His Pro Ser Ile Ile 2635 2640 2645 cca act ccg ggt tac cga ggc act cgc tac tac cgc agc cgg agc 17129 Pro Thr Pro Gly Tyr Arg Gly Thr Arg Tyr Tyr Arg Ser Arg Ser 2650 2655 2660 agc act tcc cgc cgt cgc cgc aaa aca cct gca agt cgt agt cac 17174 Ser Thr Ser Arg Arg Arg Arg Lys Thr Pro Ala Ser Arg Ser His 2665 2670 2675 cgt cgt cgt cgc cgc ccc gcc agc aat ctg acc ccc gct gct ctg 17219 Arg Arg Arg Arg Arg Pro Ala Ser Asn Leu Thr Pro Ala Ala Leu 2680 2685 2690 gtg cgg aga gtg tat cgc gat ggc cgc gca gat ccc ctg acg ttg 17264 Val Arg Arg Val Tyr Arg Asp Gly Arg Ala Asp Pro Leu Thr Leu 2695 2700 2705 cca cgc gta cgc tac cat cca agc atc aca act taacgactgt 17307 Pro Arg Val Arg Tyr His Pro Ser Ile Thr Thr 2710 2715 tgccgctgcc tccttgcaga t atg gcc ctc act tgc cgc ctt cgt gtc ccc 17358 Met Ala Leu Thr Cys Arg Leu Arg Val Pro 2720 2725 att act ggc tac cga gga aga aac tcg cgc cgt aga aga ggg atg 17403 Ile Thr Gly Tyr Arg Gly Arg Asn Ser Arg Arg Arg Arg Gly Met 2730 2735 2740 ttg ggg cgc ggg atg cga cgc cac agg cgg cgg cgc gct atc agc 17448 Leu Gly Arg Gly Met Arg Arg His Arg Arg Arg Arg Ala Ile Ser 2745 2750 2755 aaa agg ctg ggg ggt ggc ttt ctg cct gct ctg atc ccc atc ata 17493 Lys Arg Leu Gly Gly Gly Phe Leu Pro Ala Leu Ile Pro Ile Ile 2760 2765 2770 gcc gcg gcg atc ggg gcg ata cca ggc ata gct tcc gtg gcg gtt 17538 Ala Ala Ala Ile Gly Ala Ile Pro Gly Ile Ala Ser Val Ala Val 2775 2780 2785 cag gcc tcg cag cgc cac tgacattgga aaaacttata aataaaatag 17586 Gln Ala Ser Gln Arg His 2790 aatggactct gatgctcctg gtcctgtgac tatgtttttg tagag atg gaa gac 17640 Met Glu Asp 2795 atc aat ttt tca tcc ctg gct ccg cga cac ggc acg agg ccg tac 17685 Ile Asn Phe Ser Ser Leu Ala Pro Arg His Gly Thr Arg Pro Tyr 2800 2805 2810 atg ggc acc tgg agc gac atc ggc acc agc caa ctg aac ggg ggc 17730 Met Gly Thr Trp Ser Asp Ile Gly Thr Ser Gln Leu Asn Gly Gly 2815 2820 2825 gcc ttc aat tgg agc agt atc tgg agc ggg ctt aaa aat ttt ggc 17775 Ala Phe Asn Trp Ser Ser Ile Trp Ser Gly Leu Lys Asn Phe Gly 2830 2835 2840 tct acc ata aaa acc tat ggg aac aaa gct tgg aac agc agc aca 17820 Ser Thr Ile Lys Thr Tyr Gly Asn Lys Ala Trp Asn Ser Ser Thr 2845 2850 2855 ggg cag gca ctg aga aat aag ctt aaa gag caa aac ttc caa cag 17865 Gly Gln Ala Leu Arg Asn Lys Leu Lys Glu Gln Asn Phe Gln Gln 2860 2865 2870 aag gtg gtt gat ggg atc gcc tct ggt atc aat ggg gtg gtg gat 17910 Lys Val Val Asp Gly Ile Ala Ser Gly Ile Asn Gly Val Val Asp 2875 2880 2885 ctg gcc aac cag gcc gtg cag aaa cag ata aac agc cgc ctg gac 17955 Leu Ala Asn Gln Ala Val Gln Lys Gln Ile Asn Ser Arg Leu Asp 2890 2895 2900 ccg ccg ccg tca gcc ccg ggt gaa atg gaa gtg gag gaa gat ctc 18000 Pro Pro Pro Ser Ala Pro Gly Glu Met Glu Val Glu Glu Asp Leu 2905 2910 2915 cct ccc ctt gaa aag cgg ggc gac aag cgt ccg cgc ccc gat ctg 18045 Pro Pro Leu Glu Lys Arg Gly Asp Lys Arg Pro Arg Pro Asp Leu 2920 2925 2930 gag gag aca cta gtc aca cgc tca gac gac ccg ccc tcc tac gag 18090 Glu Glu Thr Leu Val Thr Arg Ser Asp Asp Pro Pro Ser Tyr Glu 2935 2940 2945 gag gca gtg aag ctt gga atg ccc acc acc aga cct gta gcc ccc 18135 Glu Ala Val Lys Leu Gly Met Pro Thr Thr Arg Pro Val Ala Pro 2950 2955 2960 atg gct acc ggg gta atg aaa cct tct cag tca cac cga ccc gct 18180 Met Ala Thr Gly Val Met Lys Pro Ser Gln Ser His Arg Pro Ala 2965 2970 2975 acc ttg gac ttg cct cct ccc cct act gct gca gcg cct gct cgc 18225 Thr Leu Asp Leu Pro Pro Pro Pro Thr Ala Ala Ala Pro Ala Arg 2980 2985 2990 aag cct gtc gct acc ccg aag ccc acc acc gta cag ccc gtc gcc 18270 Lys Pro Val Ala Thr Pro Lys Pro Thr Thr Val Gln Pro Val Ala 2995 3000 3005 gta gcc agg ccg cgt cct ggg ggc act cca cgt cca aat gca aac 18315 Val Ala Arg Pro Arg Pro Gly Gly Thr Pro Arg Pro Asn Ala Asn 3010 3015 3020 tgg cag agt act ctg aac agc atc gtg ggt ctg ggc gtg caa agt 18360 Trp Gln Ser Thr Leu Asn Ser Ile Val Gly Leu Gly Val Gln Ser 3025 3030 3035 gta aag cgc cgt cgc tgc ttt taaattaaat atggagtagc gcttaacttg 18411 Val Lys Arg Arg Arg Cys Phe 3040 cctgtctgtg tgtgtatgtg tcatcatcac gccgccgccg cagcaacagc agaggagaaa 18471 aggaagaggt cgcgcgccga ggctgagttg ctttcaag atg gcc acc cca tcg 18524 Met Ala Thr Pro Ser 3045 atg ctg ccc cag tgg gca tac atg cac atc gcc gga cag gat gct 18569 Met Leu Pro Gln Trp Ala Tyr Met His Ile Ala Gly Gln Asp Ala 3050 3055 3060 tcg gag tac ctg agt ccg ggt ctg gtg cag ttc gcc cgc gcc aca 18614 Ser Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala Arg Ala Thr 3065 3070 3075 gac acc tac ttc aat ctg ggg aac aag ttt agg aac cct acc gtg 18659 Asp Thr Tyr Phe Asn Leu Gly Asn Lys Phe Arg Asn Pro Thr Val 3080 3085 3090 gcg ccc acc cat gat gtg acc acc gac cgc agt caa cgg ctg atg 18704 Ala Pro Thr His Asp Val Thr Thr Asp Arg Ser Gln Arg Leu Met 3095 3100 3105 ctc cgc ttt gtg ccc gtt gac cgg gag gac aat acc tac tca tac 18749 Leu Arg Phe Val Pro Val Asp Arg Glu Asp Asn Thr Tyr Ser Tyr 3110 3115 3120 aaa gtt cga tac acc ttg gct gtg ggc gac aac aga gtg ctg gat 18794 Lys Val Arg Tyr Thr Leu Ala Val Gly Asp Asn Arg Val Leu Asp 3125 3130 3135 atg gcc agt act ttc ttt gac att cgg ggt gtg ttg gat aga ggc 18839 Met Ala Ser Thr Phe Phe Asp Ile Arg Gly Val Leu Asp Arg Gly 3140 3145 3150 cct agc ttc aag cca tat tct ggc act gct tac aac tca ttg gcc 18884 Pro Ser Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser Leu Ala 3155 3160 3165 cct aag ggc gct ccc aat aca tct cag tgg att gct gaa ggc gta 18929 Pro Lys Gly Ala Pro Asn Thr Ser Gln Trp Ile Ala Glu Gly Val 3170 3175 3180 aaa aaa gaa aat ggg gaa gct gac aat gaa gca gct gtc gaa gag 18974 Lys Lys Glu Asn Gly Glu Ala Asp Asn Glu Ala Ala Val Glu Glu 3185 3190 3195 gaa gag gaa gag aaa aat ctt acc act tac act ttt gga aat gcc 19019 Glu Glu Glu Glu Lys Asn Leu Thr Thr Tyr Thr Phe Gly Asn Ala 3200 3205 3210 cca gtg aaa gca gaa ggt ggt gat atc act aaa gac aaa ggt ctt 19064 Pro Val Lys Ala Glu Gly Gly Asp Ile Thr Lys Asp Lys Gly Leu 3215 3220 3225 cca att ggt tca gaa att aca gac ggc aaa gcc aaa cca att tat 19109 Pro Ile Gly Ser Glu Ile Thr Asp Gly Lys Ala Lys Pro Ile Tyr 3230 3235 3240 gca gat aaa cta tac caa cca gaa cct cag gtg gga gag gaa act 19154 Ala Asp Lys Leu Tyr Gln Pro Glu Pro Gln Val Gly Glu Glu Thr 3245 3250 3255 tgg act gac aca gat gga aca act gag aag tat ggt ggt aga gct 19199 Trp Thr Asp Thr Asp Gly Thr Thr Glu Lys Tyr Gly Gly Arg Ala 3260 3265 3270 cta aag cca gaa act aaa atg aaa ccc tgc tat ggg tct ttt gct 19244 Leu Lys Pro Glu Thr Lys Met Lys Pro Cys Tyr Gly Ser Phe Ala 3275 3280 3285 aaa ccc act aac gtc aaa ggc gga cag gca aaa caa aaa act act 19289 Lys Pro Thr Asn Val Lys Gly Gly Gln Ala Lys Gln Lys Thr Thr 3290 3295 3300 gaa caa ctg caa aac cag cag gtt gaa tat gat att gac atg aac 19334 Glu Gln Leu Gln Asn Gln Gln Val Glu Tyr Asp Ile Asp Met Asn 3305 3310 3315 ttt ttt gat caa gcg tca cag aaa gca aac ttc agt cca aaa att 19379 Phe Phe Asp Gln Ala Ser Gln Lys Ala Asn Phe Ser Pro Lys Ile 3320 3325 3330 gtg atg tat gca gaa aat gta gac ttg gaa acc cca gac act cac 19424 Val Met Tyr Ala Glu Asn Val Asp Leu Glu Thr Pro Asp Thr His 3335 3340 3345 gtg gtg tac aaa cct ggt act tca gaa gaa agt tct cat gct aat 19469 Val Val Tyr Lys Pro Gly Thr Ser Glu Glu Ser Ser His Ala Asn 3350 3355 3360 ctc ggt caa caa tct atg ccc aac aga ccc aac tac att ggc ttt 19514 Leu Gly Gln Gln Ser Met Pro Asn Arg Pro Asn Tyr Ile Gly Phe 3365 3370 3375 aga gat aac ttt att gga ctt atg tac tac aac agt act ggc aac 19559 Arg Asp Asn Phe Ile Gly Leu Met Tyr Tyr Asn Ser Thr Gly Asn 3380 3385 3390 atg gga gtg ctg gca ggt caa gca tcc caa ttg aat gcg gtg gtt 19604 Met Gly Val Leu Ala Gly Gln Ala Ser Gln Leu Asn Ala Val Val 3395 3400 3405 gac ttg cag gac aga aac aca gaa cta tca tat caa cta ctg ctt 19649 Asp Leu Gln Asp Arg Asn Thr Glu Leu Ser Tyr Gln Leu Leu Leu 3410 3415 3420 gat tct ctg ggt gac aga acc aga tac ttc agc atg tgg aat caa 19694 Asp Ser Leu Gly Asp Arg Thr Arg Tyr Phe Ser Met Trp Asn Gln 3425 3430 3435 gca gtc gat agc tat gat cct gat gtg cgc att att gaa aat cat 19739 Ala Val Asp Ser Tyr Asp Pro Asp Val Arg Ile Ile Glu Asn His 3440 3445 3450 ggg gtg gaa gat gag ctt ccc aac tac tgc ttt cca ttg gat gga 19784 Gly Val Glu Asp Glu Leu Pro Asn Tyr Cys Phe Pro Leu Asp Gly 3455 3460 3465 gta ggg gta cca aca act agt tac aaa ata att gaa cca aat gga 19829 Val Gly Val Pro Thr Thr Ser Tyr Lys Ile Ile Glu Pro Asn Gly 3470 3475 3480 gag ggt gca gat tgg aaa gag cct gac ata aat gga aca agt gaa 19874 Glu Gly Ala Asp Trp Lys Glu Pro Asp Ile Asn Gly Thr Ser Glu 3485 3490 3495 att gga caa gga aat ctc ttt gcc atg gaa att aac ctc caa gct 19919 Ile Gly Gln Gly Asn Leu Phe Ala Met Glu Ile Asn Leu Gln Ala 3500 3505 3510 aat ctc tgg aga agt ttt ctt tat tcc aat gtg gct ctg tat ctc 19964 Asn Leu Trp Arg Ser Phe Leu Tyr Ser Asn Val Ala Leu Tyr Leu 3515 3520 3525 cca gac tcc tac aaa tac acc cca gcc aat gtc act ctt cca act 20009 Pro Asp Ser Tyr Lys Tyr Thr Pro Ala Asn Val Thr Leu Pro Thr 3530 3535 3540 aac acc aac act tat gac tac atg aat ggg cgg gtg gtt ccc cca 20054 Asn Thr Asn Thr Tyr Asp Tyr Met Asn Gly Arg Val Val Pro Pro 3545 3550 3555 tcc cta gtg gat acc tac gta aac att ggc gcc aga tgg tct ttg 20099 Ser Leu Val Asp Thr Tyr Val Asn Ile Gly Ala Arg Trp Ser Leu 3560 3565 3570 gat gcc atg gac aat gtc aac ccc ttt aac cat cac cgc aac gct 20144 Asp Ala Met Asp Asn Val Asn Pro Phe Asn His His Arg Asn Ala 3575 3580 3585 ggc ctg cga tac cgg tcc atg ctt ttg ggc aat ggt cgc tac gtg 20189 Gly Leu Arg Tyr Arg Ser Met Leu Leu Gly Asn Gly Arg Tyr Val 3590 3595 3600 cct ttc cac att caa gtg cct cag aaa ttc ttt gct gtg aag aac 20234 Pro Phe His Ile Gln Val Pro Gln Lys Phe Phe Ala Val Lys Asn 3605 3610 3615 ctg ctg ctt cta ccc ggt tct tac acc tac gag tgg aac ttc aga 20279 Leu Leu Leu Leu Pro Gly Ser Tyr Thr Tyr Glu Trp Asn Phe Arg 3620 3625 3630 aag gat gtg aac atg gtc ctg cag agt tcc ctt ggt aat gat ctc 20324 Lys Asp Val Asn Met Val Leu Gln Ser Ser Leu Gly Asn Asp Leu 3635 3640 3645 cgg gtc gat ggt gcc agc atc agt ttt acc agc atc aat ctc tat 20369 Arg Val Asp Gly Ala Ser Ile Ser Phe Thr Ser Ile Asn Leu Tyr 3650 3655 3660 gcc acc ttc ttc ccc atg gcc cac aac act gcc tcc acc ctt gaa 20414 Ala Thr Phe Phe Pro Met Ala His Asn Thr Ala Ser Thr Leu Glu 3665 3670 3675 gcc atg ctg cgc aat gac acc aat gat caa tca ttc aat gac tac 20459 Ala Met Leu Arg Asn Asp Thr Asn Asp Gln Ser Phe Asn Asp Tyr 3680 3685 3690 ctt tct gca gcc aac atg ctc tac ccc atc ccg gcc aac gct acc 20504 Leu Ser Ala Ala Asn Met Leu Tyr Pro Ile Pro Ala Asn Ala Thr 3695 3700 3705 aac gtt ccc atc tcc att ccc tct cgt aac tgg gcc gcc ttc aga 20549 Asn Val Pro Ile Ser Ile Pro Ser Arg Asn Trp Ala Ala Phe Arg 3710 3715 3720 ggc tgg tcc ttc acc aga ctc aaa acc aaa gag act ccc tct ttg 20594 Gly Trp Ser Phe Thr Arg Leu Lys Thr Lys Glu Thr Pro Ser Leu 3725 3730 3735 gga tca ggg ttc gat ccc tac ttt gtt tac tct ggt tct ata cct 20639 Gly Ser Gly Phe Asp Pro Tyr Phe Val Tyr Ser Gly Ser Ile Pro 3740 3745 3750 tac ctg gat ggt acc ttc tac ctt aac cac act ttt aag aaa gtc 20684 Tyr Leu Asp Gly Thr Phe Tyr Leu Asn His Thr Phe Lys Lys Val 3755 3760 3765 tct atc atg ttt gac tct tca gtc agc tgg cct ggt aat gac aga 20729 Ser Ile Met Phe Asp Ser Ser Val Ser Trp Pro Gly Asn Asp Arg 3770 3775 3780 ttg cta act cca aat gag ttc gaa atc aag cgc aca gtt gat ggg 20774 Leu Leu Thr Pro Asn Glu Phe Glu Ile Lys Arg Thr Val Asp Gly 3785 3790 3795 gaa ggc tac aat gtg gcc caa tgt aac atg acc aaa gac tgg ttc 20819 Glu Gly Tyr Asn Val Ala Gln Cys Asn Met Thr Lys Asp Trp Phe 3800 3805 3810 ctg gtc cag atg ctt gcc aac tac aac att gga tac cag ggc ttc 20864 Leu Val Gln Met Leu Ala Asn Tyr Asn Ile Gly Tyr Gln Gly Phe 3815 3820 3825 tac gtt cct gag ggt tac aag gat cgc atg tac tcc ttc ttc aga 20909 Tyr Val Pro Glu Gly Tyr Lys Asp Arg Met Tyr Ser Phe Phe Arg 3830 3835 3840 aac ttc cag ccc atg agt aga cag gtg gtt gat gag att aac tac 20954 Asn Phe Gln Pro Met Ser Arg Gln Val Val Asp Glu Ile Asn Tyr 3845 3850 3855 aaa gac tat aaa gct gtc gcc gta ccc tac cag cat aat aac tct 20999 Lys Asp Tyr Lys Ala Val Ala Val Pro Tyr Gln His Asn Asn Ser 3860 3865 3870 ggc ttt gtg ggt tac atg gct cct acc atg cgt cag ggt caa gcg 21044 Gly Phe Val Gly Tyr Met Ala Pro Thr Met Arg Gln Gly Gln Ala 3875 3880 3885 tac cct gct aac tac cca tac ccc cta att gga acc act gca gta 21089 Tyr Pro Ala Asn Tyr Pro Tyr Pro Leu Ile Gly Thr Thr Ala Val 3890 3895 3900 acc agt gtc acc cag aaa aaa ttc ctg tgc gac agg acc atg tgg 21134 Thr Ser Val Thr Gln Lys Lys Phe Leu Cys Asp Arg Thr Met Trp 3905 3910 3915 cgc atc cca ttc tct agc aac ttc atg tcc atg ggt gcc ctt aca 21179 Arg Ile Pro Phe Ser Ser Asn Phe Met Ser Met Gly Ala Leu Thr 3920 3925 3930 gac ctg gga cag aac ttg ctg tat gcc aac tca gcc cat gcg ctg 21224 Asp Leu Gly Gln Asn Leu Leu Tyr Ala Asn Ser Ala His Ala Leu 3935 3940 3945 gac atg act ttt gag gtg gat ccc atg gat gag ccc acc ctg ctt 21269 Asp Met Thr Phe Glu Val Asp Pro Met Asp Glu Pro Thr Leu Leu 3950 3955 3960 tat ctt ctt ttt gaa gta ttc gac gtg gtc aga gtg cac caa cca 21314 Tyr Leu Leu Phe Glu Val Phe Asp Val Val Arg Val His Gln Pro 3965 3970 3975 cac cgc ggc gtc atc gag gcc gtc tac ctg cgc aca ccg ttc tcg 21359 His Arg Gly Val Ile Glu Ala Val Tyr Leu Arg Thr Pro Phe Ser 3980 3985 3990 gct ggt aac gcc acc aca taagaaacct gcttcttgca aggtgcagcc 21407 Ala Gly Asn Ala Thr Thr 3995 atggcctgcg ggtccggaaa cggctccagc gagcaagagc tcagagccat cgtccgagac 21467 cttggctgtg gaccctactt cctgggaacc tttgacaaac gcttcccggg gtttatggct 21527 ccaaacaagc tggcctgcgc cattgtcaac acagccggtc gcgagacggg gggagagcac 21587 tggttggctt ttggttggaa cccgcgctcc aacacatgct acctttttga tccgtttgga 21647 ttctcggatg accgtctcaa gcagatctac cagtttgaat acgaggggtt actgcgccgc 21707 agcgcccttg ctactaagga tcgctgcatt accttggaaa agtccaccca aaccgtgcag 21767 ggtccgcgct ccgccgcttg tggacttttt tgctgcatgt ttctccatgc ctttgtacac 21827 tggccagacc gccccatgga cggtaacccc accatgaagt tgcttacggg agtgcccaac 21887 agcatgctcc agtcacccca agtccagccc accctgcgca ggaaccagga ggcgctctac 21947 catttcctca acacacattc atcttacttt cgttctcacc gcgcacgtat cgaaagggct 22007 actgcgttcg atcgtatggg atattaataa gtcatgtaaa accgtgttca ataaacagaa 22067 ctttattttt tacatgcact ggtggtttct cattcattta ttcactcaga agtcgaaggg 22127 gttttggcgg gaatcagagt gacccgcggg cagggatacg ttgcggaact ggaactgagc 22187 ctgccacttg aattcgggga tcaccagctt gggaactggc aggtcaggca ggatgtcgct 22247 ccacagcttc ctggtcagtt gcagggctcc caacaggtca ggagctgaaa tcttgaaatc 22307 gcaattggga cccgtgctct gagcgcggga gttgcgatac acagggttgc aacactggaa 22367 caccatcagc gacgggtatt tcacactcgc cagcacagtg ggatcggtga taattcccac 22427 atccaggtct tcggcattgg ccatgctaaa gggggtcatc ttgcatgtct gtctgcccat 22487 agccggtacc cagcctggct tgtggttgca atcgcagcgc agagggatca gcatcatctt 22547 ggcctggtcg gatctcatac cgggatacac agctttcatg aaagcttcat attgcttgaa 22607 agcctgttgg gccttgctac cctcagtgta gaacatccca caagacttgc tagagaactg 22667 gttagcagca catccggcat cattcacaca acagcgagcg tcgttgttgg ctatttgcac 22727 cacactcctg ccccagcggt tctgggtgat cttggttcgc tcagggttct ccttcagcgc 22787 ccgttgaccg ttttcgcttg ccacatccat ttctatgata tgttccttct gaatcatgat 22847 gttgccatgc aaacacttca gcttgccttc ataatcatta catccatgtg accacagcgc 22907 gcatcccgta cactcccagt tattgtgagc gatctcagaa taggaatgca ccaacccctg 22967 caggaatctt cccatcatgg ttgagagggt cttgttactg gtgaaagtca gcgggacgcc 23027 tcgatgctcc tcgttcacat actggtggca aattcgcttg tactgttcat gctgctctgg 23087 cataagcttg aaagaggttc ttaggtcatt ctccagcctg tacttctcca tcagcacagc 23147 cattacttcc atgccctttt cccaggcaga aaccaggggt aggctcatgg aatttctaac 23207 agaaatagca gctactttag ccagagggtc atccttgtca atcttctcaa cacttctttt 23267 gccatccttc tcagtgatgc gcacgggtgg gtagctgaag cccacggcca ccagctccgc 23327 ctcttctctt tcttcttcgc tgtcctgact gatgtcttgt aaagggacat gcttggtctt 23387 cctgggcttc tttttggggg gtattggcgg agggctgctg ctccgctccg gagacatgga 23447 ggaccgcgaa gtttcgctca ccagtaccac ctggctctcg gtagaagaac cggaccccac 23507 acggcggtag gtgttcctct tcgggggcag aggtggaggt gactgcgatg ggctgcggtc 23567 cggcctggga ggcggatgac tggcagagcc ccttccgcgt tcgggggtgt gctcccggtg 23627 gcggtcgctt gactgatttc ctccgcggct ggccattgtg ttctcctagg cagagaaaca 23687 acagac atg gag act cag cca tcg ctg cca aca ccg ctg caa gca cca 23735 Met Glu Thr Gln Pro Ser Leu Pro Thr Pro Leu Gln Ala Pro 4000 4005 4010 tca cac ctc gcc tcc agc gat gag gag gag gaa caa agc tta acc 23780 Ser His Leu Ala Ser Ser Asp Glu Glu Glu Glu Gln Ser Leu Thr 4015 4020 4025 gcc cca cca ccc agt ccc gcc acc acc acc tct acc ctc gag gat 23825 Ala Pro Pro Pro Ser Pro Ala Thr Thr Thr Ser Thr Leu Glu Asp 4030 4035 4040 gag gag gtc gac gca ccc cag gag ata cgg acg cag gat atg gag 23870 Glu Glu Val Asp Ala Pro Gln Glu Ile Arg Thr Gln Asp Met Glu 4045 4050 4055 gat gag aaa gcg gaa gag att gag gca gat atc gag cag gac cca 23915 Asp Glu Lys Ala Glu Glu Ile Glu Ala Asp Ile Glu Gln Asp Pro 4060 4065 4070 ggc tat gtg aca ccg gcc gag cac gag gaa gag ctg aga cgc ttt 23960 Gly Tyr Val Thr Pro Ala Glu His Glu Glu Glu Leu Arg Arg Phe 4075 4080 4085 cta gag aaa gat gat gac aac cgt cca gaa cag caa gca gat ggc 24005 Leu Glu Lys Asp Asp Asp Asn Arg Pro Glu Gln Gln Ala Asp Gly 4090 4095 4100 gat cag cag aat gtt ggg ctc ggg gat cat gtt gtc gac tac ctc 24050 Asp Gln Gln Asn Val Gly Leu Gly Asp His Val Val Asp Tyr Leu 4105 4110 4115 acc ggc ctt ggt ggg gag gac gtg ctc ctc aaa cac cta gca agg 24095 Thr Gly Leu Gly Gly Glu Asp Val Leu Leu Lys His Leu Ala Arg 4120 4125 4130 cag tcg atc ata atc aaa gat gca ctg ctt gat cgc agc gaa gtg 24140 Gln Ser Ile Ile Ile Lys Asp Ala Leu Leu Asp Arg Ser Glu Val 4135 4140 4145 ccc atc agt gtg gaa gag ctc agc cgc gcc tac gag ctc aac ctg 24185 Pro Ile Ser Val Glu Glu Leu Ser Arg Ala Tyr Glu Leu Asn Leu 4150 4155 4160 ttc tcg cct cgg gta ccc ccc aag cgt cag cca aac ggc acc tgc 24230 Phe Ser Pro Arg Val Pro Pro Lys Arg Gln Pro Asn Gly Thr Cys 4165 4170 4175 gag ccc aac cct cgc ctc aac ttc tat ccc gca ttc acc gtc ccc 24275 Glu Pro Asn Pro Arg Leu Asn Phe Tyr Pro Ala Phe Thr Val Pro 4180 4185 4190 gag gtg ctg gct acc tac cac ata ttt ttc aaa aac caa aaa att 24320 Glu Val Leu Ala Thr Tyr His Ile Phe Phe Lys Asn Gln Lys Ile 4195 4200 4205 cca att tcc tgc cgc gcc aac cga act cgc gcc gat gcc ctg ctc 24365 Pro Ile Ser Cys Arg Ala Asn Arg Thr Arg Ala Asp Ala Leu Leu 4210 4215 4220 aac ttg gga cct ggc gct tgc tta cct gat ata act tcc ttg gaa 24410 Asn Leu Gly Pro Gly Ala Cys Leu Pro Asp Ile Thr Ser Leu Glu 4225 4230 4235 gag gtc cca aag atc ttc gaa ggt ctg ggc agt gat gag act cgg 24455 Glu Val Pro Lys Ile Phe Glu Gly Leu Gly Ser Asp Glu Thr Arg 4240 4245 4250 gcc gca aat gct ctg caa cag gga gag aat ggc atc gat gaa cat 24500 Ala Ala Asn Ala Leu Gln Gln Gly Glu Asn Gly Ile Asp Glu His 4255 4260 4265 cac agc gct ctg gtg gag ttg gag ggc gat aat gcc cga cta gca 24545 His Ser Ala Leu Val Glu Leu Glu Gly Asp Asn Ala Arg Leu Ala 4270 4275 4280 gta ctc aag cgc agt atc gag gtg acc cat ttt gca tac ccc gct 24590 Val Leu Lys Arg Ser Ile Glu Val Thr His Phe Ala Tyr Pro Ala 4285 4290 4295 gtc aac ctg cct ccc aaa gtc atg agc gct gtc atg gat cag ata 24635 Val Asn Leu Pro Pro Lys Val Met Ser Ala Val Met Asp Gln Ile 4300 4305 4310 ctc att aaa cgc gca agt ccc ctt tca gaa aac atg cag gat cca 24680 Leu Ile Lys Arg Ala Ser Pro Leu Ser Glu Asn Met Gln Asp Pro 4315 4320 4325 gac gcc tcg gat gag ggc aag cca gtg gtc agt gat gaa cag cta 24725 Asp Ala Ser Asp Glu Gly Lys Pro Val Val Ser Asp Glu Gln Leu 4330 4335 4340 tct cgc tgg ctg ggc acc aac tcc cca cga gac ttg gaa gag cgg 24770 Ser Arg Trp Leu Gly Thr Asn Ser Pro Arg Asp Leu Glu Glu Arg 4345 4350 4355 cgc aag ctc atg atg gcc gtg gtg cta gtt act gtg gaa atg gag 24815 Arg Lys Leu Met Met Ala Val Val Leu Val Thr Val Glu Met Glu 4360 4365 4370 tgt ctt cgc cgc ttc ttc act gac ccc gag aca ctg cgc aag ctc 24860 Cys Leu Arg Arg Phe Phe Thr Asp Pro Glu Thr Leu Arg Lys Leu 4375 4380 4385 gag gag aac cta cac tac act ttt aga cat gga ttt gtg aga cag 24905 Glu Glu Asn Leu His Tyr Thr Phe Arg His Gly Phe Val Arg Gln 4390 4395 4400 gca tgc aag atc tcc aac gtg gag ctt acc aac ctg gtt tcc tac 24950 Ala Cys Lys Ile Ser Asn Val Glu Leu Thr Asn Leu Val Ser Tyr 4405 4410 4415 atg ggc att ttg cat gaa aac aga ctc gga cag agc gtg ctg cac 24995 Met Gly Ile Leu His Glu Asn Arg Leu Gly Gln Ser Val Leu His 4420 4425 4430 acc acc ctg aag ggg gaa gcc cgt cgc gac tac atc cgc gac act 25040 Thr Thr Leu Lys Gly Glu Ala Arg Arg Asp Tyr Ile Arg Asp Thr 4435 4440 4445 gtc tac ctc tac ctc tgc cat acc tgg cag act ggt atg ggt gtg 25085 Val Tyr Leu Tyr Leu Cys His Thr Trp Gln Thr Gly Met Gly Val 4450 4455 4460 tgg cag cag tgt ttg gaa gaa caa aac ctg aaa gaa cta gac aag 25130 Trp Gln Gln Cys Leu Glu Glu Gln Asn Leu Lys Glu Leu Asp Lys 4465 4470 4475 ctc tta cag aga tcc ctc aaa acc ttg tgg acg ggt ttt gac gag 25175 Leu Leu Gln Arg Ser Leu Lys Thr Leu Trp Thr Gly Phe Asp Glu 4480 4485 4490 cgc aca gtc gcc tct gat ctg gca gat ctc atc ttc cca gag cgt 25220 Arg Thr Val Ala Ser Asp Leu Ala Asp Leu Ile Phe Pro Glu Arg 4495 4500 4505 ctc agg act act ctg cgc aac ggg ctg cct gac ttc atg aac cag 25265 Leu Arg Thr Thr Leu Arg Asn Gly Leu Pro Asp Phe Met Asn Gln 4510 4515 4520 agc atg att aac aac ttt cgc tct ttc atc ctg gaa cgc tcc ggt 25310 Ser Met Ile Asn Asn Phe Arg Ser Phe Ile Leu Glu Arg Ser Gly 4525 4530 4535 atc ctg ccc gcc acc tgc tgt gcg cta cca tcc gac ttt gtg cct 25355 Ile Leu Pro Ala Thr Cys Cys Ala Leu Pro Ser Asp Phe Val Pro 4540 4545 4550 ctg acc tac cgc gag tgc ccc cca ccg cta tgg agc cac tgc tac 25400 Leu Thr Tyr Arg Glu Cys Pro Pro Pro Leu Trp Ser His Cys Tyr 4555 4560 4565 ctg ttc cgc ctg gcc aac tac cta tca tac cac tcg gat gtg atc 25445 Leu Phe Arg Leu Ala Asn Tyr Leu Ser Tyr His Ser Asp Val Ile 4570 4575 4580 gag gat gtg agc gga gat ggc ctg ctt gag tgc cac tgc cgc tgt 25490 Glu Asp Val Ser Gly Asp Gly Leu Leu Glu Cys His Cys Arg Cys 4585 4590 4595 aat ctc tgc tca cca cat cgc tcc ctc gtc tgt aac ccc cag ttg 25535 Asn Leu Cys Ser Pro His Arg Ser Leu Val Cys Asn Pro Gln Leu 4600 4605 4610 ctt agc gaa acc caa att ata ggc acc ttc gaa ttg cag ggt ccc 25580 Leu Ser Glu Thr Gln Ile Ile Gly Thr Phe Glu Leu Gln Gly Pro 4615 4620 4625 agc agc gaa ggc gag ggg tct tct cct ggg caa agt ttg aaa ctg 25625 Ser Ser Glu Gly Glu Gly Ser Ser Pro Gly Gln Ser Leu Lys Leu 4630 4635 4640 acc ccg gga ctg tgg acc tcc gcc tac ctg cgc aag ttc tcc ccc 25670 Thr Pro Gly Leu Trp Thr Ser Ala Tyr Leu Arg Lys Phe Ser Pro 4645 4650 4655 gag gac tac cac ccc tat gag atc agg ttc tat gaa gac caa tca 25715 Glu Asp Tyr His Pro Tyr Glu Ile Arg Phe Tyr Glu Asp Gln Ser 4660 4665 4670 cag ccg ccc aaa gct gag ctc tca gcg tgc gtc atc acc cag ggg 25760 Gln Pro Pro Lys Ala Glu Leu Ser Ala Cys Val Ile Thr Gln Gly 4675 4680 4685 gca att ttg gcc caa ttg caa gcc atc caa aaa tcc cgc caa gaa 25805 Ala Ile Leu Ala Gln Leu Gln Ala Ile Gln Lys Ser Arg Gln Glu 4690 4695 4700 ttt ttg ctg aaa aag ggt aac gga gtc tac ctc gac ccc cag act 25850 Phe Leu Leu Lys Lys Gly Asn Gly Val Tyr Leu Asp Pro Gln Thr 4705 4710 4715 ggt gag gag ctc aac aca agg ttc tct cag gat gtc tca gcg ccg 25895 Gly Glu Glu Leu Asn Thr Arg Phe Ser Gln Asp Val Ser Ala Pro 4720 4725 4730 agg aaa caa gaa gtt gaa agt gca gct gcc gcc ccc aga gga tat 25940 Arg Lys Gln Glu Val Glu Ser Ala Ala Ala Ala Pro Arg Gly Tyr 4735 4740 4745 gga gga aga ctg gga cag tca gac aga gga gat gga aga ttg gga 25985 Gly Gly Arg Leu Gly Gln Ser Asp Arg Gly Asp Gly Arg Leu Gly 4750 4755 4760 cag cca ggc aga gga gga gga gga cag cct gga gga aga cag ttt 26030 Gln Pro Gly Arg Gly Gly Gly Gly Gln Pro Gly Gly Arg Gln Phe 4765 4770 4775 gga gga gga aga cga gga ggc aga gga ggt gga aga agc aac cgc 26075 Gly Gly Gly Arg Arg Gly Gly Arg Gly Gly Gly Arg Ser Asn Arg 4780 4785 4790 cgc caa aca gtt gtc ctc ggc ggc gga gac aag caa ggc cac aga 26120 Arg Gln Thr Val Val Leu Gly Gly Gly Asp Lys Gln Gly His Arg 4795 4800 4805 caa cac cac agc tac cat ctc cgt tcc ggg tcg ggg ggt cca gca 26165 Gln His His Ser Tyr His Leu Arg Ser Gly Ser Gly Gly Pro Ala 4810 4815 4820 ccg tcc caa cag tagatgggat gagaccgggc gactcccgaa tgcgaccacc 26217 Pro Ser Gln Gln 4825 gcttctaaga ctggtaagaa ggagcggcag ggatacaagt cctggcgggg gcataagaac 26277 gctatcatat cctgcttgca tgaatgcggg ggcaacatat ccttcacccg ccgctacctg 26337 ctcttccacc acggggtgaa cttcccccgc aatgtcttgc attactaccg tcacctccac 26397 agcccctact acagccagca agcctcggca gaaaaagaca acagcagcaa gaacctccag 26457 cagaaaacca gcagcagtta gaacacccac agcaggtgca acaggaggag gactgagaat 26517 cacagcgaac gagccagcgc agacccgaga gctgagaaac cggatttttc caaccctcta 26577 tgccatcttc caacagagtc gggggcaaga gcaggaactg aaagtaaaaa accgatcttt 26637 gcgctcgctc acccgaagtt gtttgtatca caagagcgaa gaccaacttc agcgcactct 26697 cgaggacgcc gaggctctct tcaacaagta ctgcgcgctc actcttaaag agtagcccgc 26757 gcccgcgcta gctcgaaaaa aggcgggaat tacgtcaccc attggcgcct gtcctttgcc 26817 ctcgtc atg agt aaa gaa att ccc acg cct tac atg tgg agt tat caa 26865 Met Ser Lys Glu Ile Pro Thr Pro Tyr Met Trp Ser Tyr Gln 4830 4835 4840 ccc caa atg gga ctg gca gca ggc gcc tcc cag gac tac tcc acc 26910 Pro Gln Met Gly Leu Ala Ala Gly Ala Ser Gln Asp Tyr Ser Thr 4845 4850 4855 cgt atg aat tgg ctc agc gcc ggt ccc tcg atg atc tca cgg gtt 26955 Arg Met Asn Trp Leu Ser Ala Gly Pro Ser Met Ile Ser Arg Val 4860 4865 4870 aat gat ata cga gct tat cga aac caa tta ctc cta gaa cag tca 27000 Asn Asp Ile Arg Ala Tyr Arg Asn Gln Leu Leu Leu Glu Gln Ser 4875 4880 4885 gca ctt acc gcc aca ccc aga caa cac ctt aat ccc cgg aat tgg 27045 Ala Leu Thr Ala Thr Pro Arg Gln His Leu Asn Pro Arg Asn Trp 4890 4895 4900 ccc gcc gcc ctg gtg tac cag gaa acc ccc gct ccc acc acc gtc 27090 Pro Ala Ala Leu Val Tyr Gln Glu Thr Pro Ala Pro Thr Thr Val 4905 4910 4915 cta ctt cct cga gac gcc cag gcc gaa gtt cag atg act aac gca 27135 Leu Leu Pro Arg Asp Ala Gln Ala Glu Val Gln Met Thr Asn Ala 4920 4925 4930 ggt gta cag ctg gct ggc ggt tcc gcc ctg tgt cgt cac cgg cct 27180 Gly Val Gln Leu Ala Gly Gly Ser Ala Leu Cys Arg His Arg Pro 4935 4940 4945 caa cag agt ata aaa cgc ctg gtg atc aga ggc cga ggt atc cag 27225 Gln Gln Ser Ile Lys Arg Leu Val Ile Arg Gly Arg Gly Ile Gln 4950 4955 4960 ctc aac gac gag tcg gtg agc tct tcg ctt ggt cta cga cca gac 27270 Leu Asn Asp Glu Ser Val Ser Ser Ser Leu Gly Leu Arg Pro Asp 4965 4970 4975 gga gtc ttc caa att gcc ggc tgc ggg aga tct tcc ttc act cct 27315 Gly Val Phe Gln Ile Ala Gly Cys Gly Arg Ser Ser Phe Thr Pro 4980 4985 4990 cgt cag gct gta ctg act ttg gag agt tcg tca tcg cag ccc cgc 27360 Arg Gln Ala Val Leu Thr Leu Glu Ser Ser Ser Ser Gln Pro Arg 4995 5000 5005 tcg ggt ggc atc ggg act ctc caa ttt gtg gag gag ttt act ccc 27405 Ser Gly Gly Ile Gly Thr Leu Gln Phe Val Glu Glu Phe Thr Pro 5010 5015 5020 tct gtc tac ttc aac ccc ttc tcc ggc tct cct ggg cat tat ccg 27450 Ser Val Tyr Phe Asn Pro Phe Ser Gly Ser Pro Gly His Tyr Pro 5025 5030 5035 gac gag ttc ata cca aac ttc gac gca atc agc gag tca gtg gat 27495 Asp Glu Phe Ile Pro Asn Phe Asp Ala Ile Ser Glu Ser Val Asp 5040 5045 5050 ggc tat gat tg atg tct aat ggt ggc gcg gct gag cta gct cga ctg 27542 Gly Tyr Asp Met Ser Asn Gly Gly Ala Ala Glu Leu Ala Arg Leu 5055 5060 5065 cga cat cta gac cac tgc cgc cgc ttt cgc tgc ttt gcc cga gaa 27587 Arg His Leu Asp His Cys Arg Arg Phe Arg Cys Phe Ala Arg Glu 5070 5075 5080 ctc acc gag ttc atc tac ttc gaa ata ccc gag gag cac cct caa 27632 Leu Thr Glu Phe Ile Tyr Phe Glu Ile Pro Glu Glu His Pro Gln 5085 5090 5095 gga ccg gcc cac gga gtg cgt att acc atc gaa ggg ggg ata gac 27677 Gly Pro Ala His Gly Val Arg Ile Thr Ile Glu Gly Gly Ile Asp 5100 5105 5110 tct cgc ctg cat cgg atc ttc tgc cag cga ccc gtg cta atc gag 27722 Ser Arg Leu His Arg Ile Phe Cys Gln Arg Pro Val Leu Ile Glu 5115 5120 5125 cgc gac cag gga aac acc aca gtc tcc atc tac tgc atc tgt aac 27767 Arg Asp Gln Gly Asn Thr Thr Val Ser Ile Tyr Cys Ile Cys Asn 5130 5135 5140 cac ccc gga ttg cat gaa agc ctt tgc tgt ctt att tgt gct gag 27812 His Pro Gly Leu His Glu Ser Leu Cys Cys Leu Ile Cys Ala Glu 5145 5150 5155 ttt aat aaa aac tgagttaaga ctctcctacg gactaccaat tcttcaactc 27864 Phe Asn Lys Asn 5160 ggactttata acaatcagac cctccgttca agtcagaaga ccccaaccct tcctctgatc 27924 caggaatcta attctacctc cccagcacca cactttacta gccttcccga aactaacaac 27984 ctcggagctc aactgcacca cttttccaga agccttctct ctgccaatac taccactccc 28044 agaaccggag gtgagctccg tggtcttcct aataacaacc cctgggtggt aactgggttt 28104 gtaacgctag gtgtagttgc gggtgggctt gtgcttgtcc tttgctacct atacacacct 28164 tgctgtgctt atttagtaat cttgtgttgc tggtttaaga a atg ggg gcc cta 28217 Met Gly Ala Leu cta gtc gcg ctt gct tta ctt tca ctt ttg gat ctg ggc tct act 28262 Leu Val Ala Leu Ala Leu Leu Ser Leu Leu Asp Leu Gly Ser Thr 5165 5170 5175 atg cta gtt cag cct gta cta ttt gat cca tgc ctc aat ttt gat 28307 Met Leu Val Gln Pro Val Leu Phe Asp Pro Cys Leu Asn Phe Asp 5180 5185 5190 cca gac aac tgc aca ctc act ttt gct cca gag gct ggc cgc tgt 28352 Pro Asp Asn Cys Thr Leu Thr Phe Ala Pro Glu Ala Gly Arg Cys 5195 5200 5205 gga gtt ctt att agg tgc gga cgg gaa tgc agt ccc att gaa ata 28397 Gly Val Leu Ile Arg Cys Gly Arg Glu Cys Ser Pro Ile Glu Ile 5210 5215 5220 cac cac aat aac aaa att tgg aac aat acc tta ttc acc aca tgg 28442 His His Asn Asn Lys Ile Trp Asn Asn Thr Leu Phe Thr Thr Trp 5225 5230 5235 cag cca gga gac cct gag tgg tat act gtc tct gtc cgt ggt cct 28487 Gln Pro Gly Asp Pro Glu Trp Tyr Thr Val Ser Val Arg Gly Pro 5240 5245 5250 gac ggt tcc atc cgc act gct aat aac act ttt att ttt gct gag 28532 Asp Gly Ser Ile Arg Thr Ala Asn Asn Thr Phe Ile Phe Ala Glu 5255 5260 5265 atg tgc gat ctg acc atg ttc atg agc aaa cag tat aac cta tgg 28577 Met Cys Asp Leu Thr Met Phe Met Ser Lys Gln Tyr Asn Leu Trp 5270 5275 5280 cct cca agc aag gag aac att gtg gca ttc tcc att gct tat ttc 28622 Pro Pro Ser Lys Glu Asn Ile Val Ala Phe Ser Ile Ala Tyr Phe 5285 5290 5295 ttg tgt acg tgt ctc att act gct att cta tgt atc tgc ata cac 28667 Leu Cys Thr Cys Leu Ile Thr Ala Ile Leu Cys Ile Cys Ile His 5300 5305 5310 ttg ctt att tgc cac cgc cac aga aac agc aat gag gaa aaa gag 28712 Leu Leu Ile Cys His Arg His Arg Asn Ser Asn Glu Glu Lys Glu 5315 5320 5325 aaa atg cct tgagcttttt ctcatttttg tttttttttt gtttacagcc 28761 Lys Met Pro 5330 atggcttcag ttatagctct aattattgcc agcattctca ctgccgcaca gggacaaaca 28821 attgtctata ttaccttagg tcataaccac actcttatag gaccccaaat tagttcacag 28881 gttatatgga ccaaacttgg aagtgttgat tattttgaca taatctgcaa cagaactaaa 28941 ccaatatttg taacctgtaa caaacaaaat ctcaccttaa ttaatgttag cgaaatttac 29001 agcggttact attatggtta tgacagacac agcagtgaat ataaaaatta cctagttcgc 29061 ataactcaac ccaaaaccac aaaaatgcca aataaggcaa aaattcaaat ggttagcgca 29121 ttagaacatc ttacatatcc caccacaccc gatgagagaa acattccaaa ttcaatgatt 29181 gccattattg cggcggtggc agtgggaatg gcactaataa taatttgtat gttcctatat 29241 gcttgttact gtagaaagtt tcatcacaaa caggattccc tactaaattt ttgacattta 29301 attttttata cagctatggt ttccactaca gccttttttg ttattagtag ccttgcagct 29361 gtcacttatg gtcgctcaca cctcactgta actgttggct caacttgtac actacaagga 29421 ccccaagaag ggc atg tca gtt ggt gga gaa tat gat agt gga tgg ttc 29470 Met Ser Val Gly Gly Glu Tyr Asp Ser Gly Trp Phe 5335 5340 att agg cca tgt gac cag cct ggt aac aaa ttt ttc tgc aac ggg 29515 Ile Arg Pro Cys Asp Gln Pro Gly Asn Lys Phe Phe Cys Asn Gly 5345 5350 5355 aga gac ttg acc att att aac atc aca gta aat gac cag ggc ttc 29560 Arg Asp Leu Thr Ile Ile Asn Ile Thr Val Asn Asp Gln Gly Phe 5360 5365 5370 tat tat gga act aac tat aaa aat aac tta gat tac aac att atc 29605 Tyr Tyr Gly Thr Asn Tyr Lys Asn Asn Leu Asp Tyr Asn Ile Ile 5375 5380 5385 gta gtg cca gcc acc act cca gct ccc cgc aaa acc act ttc ttt 29650 Val Val Pro Ala Thr Thr Pro Ala Pro Arg Lys Thr Thr Phe Phe 5390 5395 5400 agc agc agt gcc agt att tct aaa aca gct tct gca agc ttc aaa 29695 Ser Ser Ser Ala Ser Ile Ser Lys Thr Ala Ser Ala Ser Phe Lys 5405 5410 5415 aaa ttc gct tta cgt aat tcc aca acc tct tcc act tcc aat atg 29740 Lys Phe Ala Leu Arg Asn Ser Thr Thr Ser Ser Thr Ser Asn Met 5420 5425 5430 tct aaa tca gta atc ggc atc gct gct gcc gcg ata gtg gga tta 29785 Ser Lys Ser Val Ile Gly Ile Ala Ala Ala Ala Ile Val Gly Leu 5435 5440 5445 atg att ata att ttg tgc ata atc tac tac gcc tgc tgc tat aga 29830 Met Ile Ile Ile Leu Cys Ile Ile Tyr Tyr Ala Cys Cys Tyr Arg 5450 5455 5460 aaa cat gaa caa aaa agc gat ccc ttg ctg aat ttt gat att 29872 Lys His Glu Gln Lys Ser Asp Pro Leu Leu Asn Phe Asp Ile 5465 5470 5475 taattttttt atagcatc atg aaa aaa cta agt atc cta gct ttt att ttg 29923 Met Lys Lys Leu Ser Ile Leu Ala Phe Ile Leu 5480 5485 ttt gaa aca ttt acc aat gtg cag act act tta agt cat gat ata 29968 Phe Glu Thr Phe Thr Asn Val Gln Thr Thr Leu Ser His Asp Ile 5490 5495 5500 gag aac cac act acc tct tat gtg ccc aca aac att act acc cat 30013 Glu Asn His Thr Thr Ser Tyr Val Pro Thr Asn Ile Thr Thr His 5505 5510 5515 ccc aaa cat gct atg caa cta gaa atc acc atg cta att gta gtt 30058 Pro Lys His Ala Met Gln Leu Glu Ile Thr Met Leu Ile Val Val 5520 5525 5530 gta ata ctt att cta gct atc att ttc tat ttt aca cta tgc cgc 30103 Val Ile Leu Ile Leu Ala Ile Ile Phe Tyr Phe Thr Leu Cys Arg 5535 5540 5545 caa ata cct aat att cat aaa aat tct aaa aga cgt ccc atc tat 30148 Gln Ile Pro Asn Ile His Lys Asn Ser Lys Arg Arg Pro Ile Tyr 5550 5555 5560 tgc cct gtg att agt cga ccc cat atg act cta aat gaa atc 30190 Cys Pro Val Ile Ser Arg Pro His Met Thr Leu Asn Glu Ile 5565 5570 5575 taagatcatc tatttctctt ttacagtatg gtgaacacca atc atg att cct aga 30245 Met Ile Pro Arg 5580 aat ttc ttc ttc acc ata ctc atc tgt gct ttt aat gtc tgt gcc 30290 Asn Phe Phe Phe Thr Ile Leu Ile Cys Ala Phe Asn Val Cys Ala 5585 5590 5595 acc ttc aca gca gta gcc act gca acc cca gac tgt ata gga cca 30335 Thr Phe Thr Ala Val Ala Thr Ala Thr Pro Asp Cys Ile Gly Pro 5600 5605 5610 ttt gct tca tat aca ctt ttc gct ttt gtc gct tgc acc tgc gtg 30380 Phe Ala Ser Tyr Thr Leu Phe Ala Phe Val Ala Cys Thr Cys Val 5615 5620 5625 tgt agc gta gtc tgc ctg gtt att aat ttt ttc caa ctt gta gac 30425 Cys Ser Val Val Cys Leu Val Ile Asn Phe Phe Gln Leu Val Asp 5630 5635 5640 tgg atc ttt gta cga ctt gcc tac ctg cgt cac cat ccc gaa tac 30470 Trp Ile Phe Val Arg Leu Ala Tyr Leu Arg His His Pro Glu Tyr 5645 5650 5655 cgc aat caa cat gtt gcg gca ctt ctc aga ctt att taaaaccatg 30516 Arg Asn Gln His Val Ala Ala Leu Leu Arg Leu Ile 5660 5665 caggctatac taccagtcat tctgcttctg ttgctcccct gcgatacctt aacccccgtc 30576 gctaatcgta ccccacctga acaacttaga aaatgcaaat tccaacaacc atggacattc 30636 cttgattgct accgagaaaa atctgatttc cctacatact ggattatgat cattggaatt 30696 gtcaatctag tttcttgcac actattctct ttccttgttt atcatttttt tgattttgga 30756 tggaatgccc ccaatgcact cacttaccca caagaaccag aggaacatat cccactacag 30816 aacatgcaac agccaatagc tataatagat tatgacaatg agccacagcc ctcgctgctt 30876 cctgctatta gttacttcaa cctaaccggt ggag atg act gac cca ctc gcc 30928 Met Thr Asp Pro Leu Ala 5670 5675 gcc tcc act gct gcc gag gaa cta ctt gat atg gac ggc cgc gcc 30973 Ala Ser Thr Ala Ala Glu Glu Leu Leu Asp Met Asp Gly Arg Ala 5680 5685 5690 tca gaa cag cga ctc gcc cga cta cgc ata cgc cag cag cag gaa 31018 Ser Glu Gln Arg Leu Ala Arg Leu Arg Ile Arg Gln Gln Gln Glu 5695 5700 5705 cgt gcc gcc aag gag ctc agg gat gct att gaa att cac cag tgc 31063 Arg Ala Ala Lys Glu Leu Arg Asp Ala Ile Glu Ile His Gln Cys 5710 5715 5720 aaa aaa ggc ata ttc tgt ctg gtg aaa caa gcc aag att tcc tac 31108 Lys Lys Gly Ile Phe Cys Leu Val Lys Gln Ala Lys Ile Ser Tyr 5725 5730 5735 gag att acc aat act gac cat cgc ctc tca tac gag ctc gga ccg 31153 Glu Ile Thr Asn Thr Asp His Arg Leu Ser Tyr Glu Leu Gly Pro 5740 5745 5750 cag cgg caa aaa ttc act tgt atg gtg gga atc aac ccc ata atc 31198 Gln Arg Gln Lys Phe Thr Cys Met Val Gly Ile Asn Pro Ile Ile 5755 5760 5765 atc acc cag caa gct gga gat acc aag ggt tgc atc cac tgt tcc 31243 Ile Thr Gln Gln Ala Gly Asp Thr Lys Gly Cys Ile His Cys Ser 5770 5775 5780 tgc agt tcc acc gag tgc atc tac acc cta ctg aag acc ctc tgc 31288 Cys Ser Ser Thr Glu Cys Ile Tyr Thr Leu Leu Lys Thr Leu Cys 5785 5790 5795 ggc ctt cga gac ctc ata ccc atg aac taatcaaccc agcccctcac 31335 Gly Leu Arg Asp Leu Ile Pro Met Asn 5800 ttaccaatta cataaagcca attaataaaa atcacttact tgaaatcaga aataaggttt 31395 ctgtctacgt tgtttccaag cagcacctca cttccctctt cccaactctg gtactctaag 31455 cctcggcggg tggcatactt cctccacact ttgaaaggga tgtcaaattt tagttcctct 31515 tctttgccca caatcttcat ttctttatcc ccag atg gcc aaa cgg gct cgg 31567 Met Ala Lys Arg Ala Arg 5805 5810 cta agc agc tca ttc aat ccg gtc tac cca tat gaa gat gaa agc 31612 Leu Ser Ser Ser Phe Asn Pro Val Tyr Pro Tyr Glu Asp Glu Ser 5815 5820 5825 agc tca caa cac ccc ttt ata aac cct ggt ttc att tcc tca aat 31657 Ser Ser Gln His Pro Phe Ile Asn Pro Gly Phe Ile Ser Ser Asn 5830 5835 5840 ggt ttt aca caa agc cca gat gga gtt cta act ctt aaa tgt gtt 31702 Gly Phe Thr Gln Ser Pro Asp Gly Val Leu Thr Leu Lys Cys Val 5845 5850 5855 aat ccg ctc act acc gcc agc gga ccc ctc caa ctt aaa gtt gga 31747 Asn Pro Leu Thr Thr Ala Ser Gly Pro Leu Gln Leu Lys Val Gly 5860 5865 5870 agc agt ctt aca gta gat act atc gat ggg tct ttg gag gaa aat 31792 Ser Ser Leu Thr Val Asp Thr Ile Asp Gly Ser Leu Glu Glu Asn 5875 5880 5885 ata act gcc gca gcg cca ctc act aaa act aac cac tcc ata ggt 31837 Ile Thr Ala Ala Ala Pro Leu Thr Lys Thr Asn His Ser Ile Gly 5890 5895 5900 tta tca ata gga tct ggc ttg caa aca aag gat gat aaa ctt tgt 31882 Leu Ser Ile Gly Ser Gly Leu Gln Thr Lys Asp Asp Lys Leu Cys 5905 5910 5915 tta tcg ctg gga gat ggg ttg gta aca aag gat gat aaa cta tgt 31927 Leu Ser Leu Gly Asp Gly Leu Val Thr Lys Asp Asp Lys Leu Cys 5920 5925 5930 tta tcg ctg gga gat ggg tta ata aca aaa gat gat aca cta tgt 31972 Leu Ser Leu Gly Asp Gly Leu Ile Thr Lys Asp Asp Thr Leu Cys 5935 5940 5945 gcc aaa cta gga cat ggc ctt gtg ttt gac tct tcc aat gct atc 32017 Ala Lys Leu Gly His Gly Leu Val Phe Asp Ser Ser Asn Ala Ile 5950 5955 5960 acc ata gaa aac aac acc ttg tgg aca ggt gca aaa cca agc gcc 32062 Thr Ile Glu Asn Asn Thr Leu Trp Thr Gly Ala Lys Pro Ser Ala 5965 5970 5975 aac tgt gta att aaa gag gga gaa gat tcc cca gac tgt aag ctc 32107 Asn Cys Val Ile Lys Glu Gly Glu Asp Ser Pro Asp Cys Lys Leu 5980 5985 5990 act tta gtt cta gtg aag aat gga gga ctg ata aat gga tac ata 32152 Thr Leu Val Leu Val Lys Asn Gly Gly Leu Ile Asn Gly Tyr Ile 5995 6000 6005 aca tta atg gga gac tca gaa tat act aac acc ttg ttt aaa aac 32197 Thr Leu Met Gly Asp Ser Glu Tyr Thr Asn Thr Leu Phe Lys Asn 6010 6015 6020 aaa caa gtt aca atc gat gta aac ctc gca ttt gat aat acc ggc 32242 Lys Gln Val Thr Ile Asp Val Asn Leu Ala Phe Asp Asn Thr Gly 6025 6030 6035 caa att atc act tac cta tca tct ctt aaa agt aac ctg aac ttc 32287 Gln Ile Ile Thr Tyr Leu Ser Ser Leu Lys Ser Asn Leu Asn Phe 6040 6045 6050 aaa gac aac caa aac atg gct act gga acc ata acc agt gcc aaa 32332 Lys Asp Asn Gln Asn Met Ala Thr Gly Thr Ile Thr Ser Ala Lys 6055 6060 6065 ggc ttc atg cca agc acc act gcc tat cca ttt ata aca tac gcc 32377 Gly Phe Met Pro Ser Thr Thr Ala Tyr Pro Phe Ile Thr Tyr Ala 6070 6075 6080 act cag tcc cta aat gaa gat tac att tat gga gag tgt tac tac 32422 Thr Gln Ser Leu Asn Glu Asp Tyr Ile Tyr Gly Glu Cys Tyr Tyr 6085 6090 6095 aaa tct acc aat gga act ctc ttt cca cta aaa gtt act gtc aca 32467 Lys Ser Thr Asn Gly Thr Leu Phe Pro Leu Lys Val Thr Val Thr 6100 6105 6110 cta aac aga cgt atg tca gct tct gga atg gcc tat gct atg aat 32512 Leu Asn Arg Arg Met Ser Ala Ser Gly Met Ala Tyr Ala Met Asn 6115 6120 6125 ttt tca tgg tct cta aat gca gag gaa gcc ccg gaa act acc gaa 32557 Phe Ser Trp Ser Leu Asn Ala Glu Glu Ala Pro Glu Thr Thr Glu 6130 6135 6140 gtc act ctc att acc tcc ccc ttc ttt ttt tct tac atc aga gaa 32602 Val Thr Leu Ile Thr Ser Pro Phe Phe Phe Ser Tyr Ile Arg Glu 6145 6150 6155 gat gac tgacaacaaa aataaagatc aactttttta ttgaaaatca gtttacaaga 32658 Asp Asp ttcgagtagt tattttgccc ccctcttccc attttataga atacacaatt ctctccccac 32718 gcacagcttt gaacatttga attccattag agatagacat agttttagat tccacattcc 32778 acacagtttc agagcgggcc aatcttggat cagtgataga tataaagcca tcggaacagt 32838 ctttcaaggt ggtttcacag tccaactgct gcggctgcgg ttccggagtt tggattagag 32898 tcatctggaa gaagaacgat gggagtcata atccgagaac ggtatcgggc ggttgtgtct 32958 caaacctcga agcagtcgct gtctgcgccg ctccgtgcga ctgctgctga tgggatcagg 33018 atccacagtc tctcgaagca tgattttaat agccctcaac attaacatcc tggtgcgatg 33078 tgcacaacaa cgcattctaa tctcacttag ctcactacag taggtacaac acattaccac 33138 aatgttgttt aacaggccat aattaaaggt gctccagcca aaactcatct cagggataat 33198 catacccgcg tgaccatcgt accagatctt aatgtaaatc aaatggcgcc ccctccagaa 33258 cacactgccc acatacataa tctccttggg catatgcatg ttcacaatct ctctatacca 33318 tggacagcgc tggttaatca tacagcccat aataaccttc cggaaccaaa tagccagcaa 33378 tgctccccca gcaatacatt gaagagaacc aggctgttta cagtgacaat gaagaaccca 33438 cttctctcgc ccatggatca cttgagaatg aaatatatct atagtagcac aacacaaaca 33498 taaatgcatg catcttttca taactcttaa ctcttcgggg gttagaaaca tatcccaggg 33558 aatgggaagc tcttgcaaaa cagtaaagct ggcagaacaa ggaagaccgc gaacataact 33618 tacactgtgc atggtcaggg tattacaatc tggtaacagc ggatggtctt cagtcataga 33678 agctctggtt tcattttcct cacagcgtgg taaaggggcc ctcaaatgag ggtccatgat 33738 gtacggatga tgtctgtgac atgacgtcga tcgtgcacgc gacctcgttg taatggagct 33798 gcttcctgac attctcgtat tttgcatgac agaacctagc cttagcacaa cacacttctc 33858 ttcgccttct atcacgccgc ctagcgcgtt cagtgtggta attgaagtac agccattccc 33918 gtagatttgt caaaagttcc tcagcttcag ttgttatgaa aactccatca tatctgatcg 33978 ctctgataaa atcattcact gtagaatggg caatgcccaa ccatgcaata caattagctt 34038 gagtttcaac caaaggaggg ggaggaagac atggaagaac cataattaat tttttatgcc 34098 agacgatctc gcagtatttc taaatgaaga tcacgaagat ggcacctctc gcccccactg 34158 tgttgatgaa aaataacagc taagtcaaac acgatgcgat tttcaagatg ctcaatggtg 34218 gcttcaagca aagcctccac acgcacatcc aaaaacaaaa gaacagcaaa agaaggagca 34278 tgttctaatt cctcaatcat catattacat tcctgtacca ttcccagata attttcatct 34338 ttccagcctt gaattaatcg tgtcatttct tcttgtaaat ccaatccaca catgagaaag 34398 agctctcgga gggcaccctc caccaccatc cttaagcaca ccctcataat gacaaaatat 34458 cttgctcctg tgtcacctgc agcaaattga gaatggcaac atcaaacgac atgccattgg 34518 ctctaagctc ttctctaagt tcaagttgta aaaactcctt caaatcatcg ccaaactgct 34578 tggccatagg tccgccagga ataagagcgg gggacgctac tgtacagaac aagcggagac 34638 ctccccagtg agatccagca aaagtgaggt tacaataagc atactgagaa cctccagtga 34698 tatcatccaa tgtgctggaa acataatcag gcagagtttc tcgtataaaa ttaataaaag 34758 aaaattctgc cagatgaaca tttaaaagtt ctggaataca gatgcaataa gttaccgcgc 34818 tgcgctccaa cattgttagt acgattagtc tgtaaaaaaa gcacaaaaaa attacatcat 34878 gctagcctgg cgaacggatg gataaatcac tctctccaac accaggcagg ctacagggtc 34938 cccaacgcga ccctcgtaaa acctgtcagt atgattaaaa agcatcaccg aaagaggctg 34998 ttgatgagca gcgaatatta tttgcgatga agcatacaat ccagaagtgt tagtatcagt 35058 taaagaaaaa aaacgtccaa tatagcatct gggaacaatt atgctcaatc tcaaatgcag 35118 caaagcgaca cctctcggat gcaaagtaaa atccacagga gcataaaaaa tgtaattatt 35178 cccctcttgc acaggcagcc tagctcccgg cccctccaaa atcacataca aaacttcagc 35238 agccatagct taccgcacaa atcaggcaca gcagtcagga aaactataaa ctgactgccg 35298 cctgtgcgca atatatagag aacctataca ctgacgtaat cgaacaaagt ctaaaaaaaa 35358 atcccgccaa aaccagcaca cgcccaaaaa ctgtgtcatc cactaaaaaa aatctcactt 35418 cctcattccg taaaatcgtc acgtcctctt tcccacgaaa cgtcacttcc ggccatcttg 35478 taacgtcacc ttcccgcgcc gccccgtgac cgttgaaccc caagccaatc cccttccgct 35538 ctccattttc aaaccacctc atttacatat tggcaccatt ccatctataa ggtatattat 35598 tgatgatg 35606 <210> 167 <211> 495 <212> PRT <213> Simian adenovirus 35 <400> 167 Met Asp Pro Thr Asp Pro Leu Gln Gln Gly Ile Arg Phe Gly Phe His 1 5 10 15 Ser Ser Ser Phe Val Glu Asn Met Glu Gly Ser Gln Asp Glu Asp Asn 20 25 30 Leu Arg Leu Leu Ala Ser Thr Ala Ser Gly Arg Ser Arg Asp Pro Glu 35 40 45 Thr Pro Thr Asp His Ala Ser Gly Phe Gly Gly Gly Ala Pro Arg Gly 50 55 60 Gln Ser Glu Ser Arg Pro Gly Pro Ser Gly Gly Gly Gly Gly Gly Val 65 70 75 80 Ala Asp Leu Phe Pro Glu Leu Arg Arg Val Leu Thr Arg Ser Thr Thr 85 90 95 Ser Gly Arg Asp Arg Gly Ile Lys Arg Glu Arg Asn Pro Ser Gly Thr 100 105 110 Asn Pro Arg Ser Glu Leu Ala Leu Ser Leu Met Ser Arg Arg Arg Pro 115 120 125 Glu Thr Ile Trp Trp His Glu Val Gln Asn Glu Gly Arg Asp Glu Val 130 135 140 Ser Ile Leu Gln Glu Lys Tyr Ser Leu Glu Gln Val Lys Thr Cys Trp 145 150 155 160 Leu Glu Pro Glu Asp Asp Trp Glu Val Ala Ile Arg Asn Tyr Ala Lys 165 170 175 Ile Ala Leu Arg Pro Asp Lys Leu Tyr Arg Ile Thr Lys Arg Ile Asn 180 185 190 Ile Arg Asn Ala Cys Tyr Ile Ser Gly Asn Gly Ala Glu Val Val Ile 195 200 205 Asp Thr Gln Asp Arg Thr Val Phe Arg Cys Cys Met Met Gly Met Trp 210 215 220 Pro Gly Val Val Gly Met Glu Ala Val Thr Leu Met Asn Val Lys Phe 225 230 235 240 Arg Gly Asp Gly Tyr Asn Gly Val Val Phe Met Ala Asn Thr Lys Leu 245 250 255 Ile Leu His Gly Cys Ser Phe Phe Gly Phe Asn Asn Ile Cys Val Glu 260 265 270 Ala Trp Gly Gln Val Ser Val Arg Gly Cys Ser Phe Tyr Ala Cys Trp 275 280 285 Ile Ala Thr Ser Gly Arg Thr Lys Ser Gln Leu Ser Val Lys Lys Cys 290 295 300 Met Phe Glu Arg Cys Asn Leu Gly Ile Leu Asn Glu Gly Glu Ala Arg 305 310 315 320 Val Ser His Cys Ala Ser Ser Glu Thr Gly Cys Phe Ile Leu Ile Lys 325 330 335 Gly Asn Ala Asn Val Lys His Asn Met Ile Cys Gly Pro Ser Asp Glu 340 345 350 Arg Pro Tyr Gln Met Leu Thr Cys Ala Gly Gly His Cys Asn Met Leu 355 360 365 Ala Thr Val His Ile Val Ser His Pro Arg Lys Lys Trp Pro Val Leu 370 375 380 Glu His Asn Val Met Thr Lys Cys Thr Met His Val Gly Gly Arg Arg 385 390 395 400 Gly Met Leu Met Pro Tyr Gln Cys Asn Met Asn Asn Val Lys Val Met 405 410 415 Leu Glu Pro Asp Ala Phe Ser Arg Met Ser Leu Thr Gly Ile Phe Asp 420 425 430 Met Asn Leu Gln Ile Trp Lys Ile Leu Arg Tyr Asp Asp Thr Lys Ser 435 440 445 Arg Val Arg Ala Cys Glu Cys Gly Gly Lys His Ala Arg Phe Gln Pro 450 455 460 Val Cys Val Asp Val Thr Glu Glu Leu Arg Pro Asp His Leu Val Ile 465 470 475 480 Ala Cys Thr Gly Ala Glu Phe Gly Ser Ser Gly Glu Glu Thr Asp 485 490 495 <210> 168 <211> 138 <212> PRT <213> Simian adenovirus 35 <400> 168 Met Ser Gly Ser Ala Ser Phe Glu Gly Gly Val Phe Ser Pro Tyr Leu 1 5 10 15 Thr Gly Arg Leu Pro Pro Trp Ala Gly Val Arg Gln Asn Val Met Gly 20 25 30 Ser Thr Val Asp Gly Arg Pro Val Gln Pro Ala Asn Ser Ser Thr Leu 35 40 45 Thr Tyr Ala Thr Leu Ser Ser Ser Pro Leu Asp Ala Ala Ala Ala Ala 50 55 60 Ala Ala Ser Ala Ala Ala Asn Thr Val Leu Gly Ile Gly Tyr Tyr Gly 65 70 75 80 Ser Ile Val Ala Asn Thr Ser Ser Ser Asn Asn Pro Ser Thr Leu Ala 85 90 95 Glu Asp Lys Leu Leu Val Leu Leu Ala Gln Leu Glu Ala Leu Thr Gln 100 105 110 Arg Leu Gly Glu Leu Ser Gln Gln Val Ala Gln Leu Arg Glu Gln Thr 115 120 125 Glu Ser Ala Val Ala Thr Ala Lys Ser Lys 130 135 <210> 169 <211> 389 <212> PRT <213> Simian adenovirus 35 <400> 169 Met His Pro Val Leu Arg Gln Met Arg Pro Gln Gln Gln Pro Pro Ser 1 5 10 15 Gln Gln Gln Leu Gln Gln Gln Pro Gln Lys Ala Leu Pro Ala Pro Val 20 25 30 Thr Thr Ala Ala Ala Ala Val Ser Gly Ala Gly Gln Pro Ala Tyr Asp 35 40 45 Leu Asp Leu Glu Glu Gly Glu Gly Leu Ala Arg Leu Gly Ala Pro Ser 50 55 60 Pro Glu Arg His Pro Arg Val Gln Leu Lys Lys Asp Ser Arg Glu Ala 65 70 75 80 Tyr Val Pro Gln Gln Asn Leu Phe Arg Asp Arg Ser Gly Glu Glu Pro 85 90 95 Glu Glu Met Arg Ala Ser Arg Phe Asn Ala Gly Arg Glu Leu Arg His 100 105 110 Gly Leu Asp Arg Arg Arg Val Leu Arg Asp Asp Asp Phe Glu Val Asp 115 120 125 Glu Val Thr Gly Ile Ser Pro Ala Arg Ala His Val Ala Ala Ala Asn 130 135 140 Leu Val Ser Ala Tyr Glu Gln Thr Val Lys Glu Glu Arg Asn Phe Gln 145 150 155 160 Lys Ser Phe Asn Asn His Val Arg Thr Leu Ile Ala Arg Glu Glu Val 165 170 175 Thr Leu Gly Leu Met His Leu Trp Asp Leu Met Glu Ala Ile Thr Gln 180 185 190 Asn Pro Thr Ser Lys Pro Leu Thr Ala Gln Leu Phe Leu Val Val Gln 195 200 205 His Ser Arg Asp Asn Glu Ala Phe Arg Glu Ala Leu Leu Asn Ile Thr 210 215 220 Glu Pro Glu Gly Arg Trp Leu Tyr Asp Leu Ile Asn Ile Leu Gln Ser 225 230 235 240 Ile Ile Val Gln Glu Arg Ser Leu Gly Leu Ala Glu Lys Val Ala Ala 245 250 255 Ile Asn Tyr Ser Val Leu Ser Leu Gly Lys His Tyr Ala Arg Lys Ile 260 265 270 Tyr Lys Thr Pro Tyr Val Pro Ile Asp Lys Glu Val Lys Ile Asp Gly 275 280 285 Phe Tyr Met Arg Met Thr Leu Lys Val Leu Thr Leu Ser Asp Asp Leu 290 295 300 Gly Val Tyr Arg Asn Asp Arg Met His Arg Ala Val Ser Ala Ser Arg 305 310 315 320 Arg Arg Glu Leu Ser Asp Arg Glu Leu Met His Ser Leu Gln Arg Ala 325 330 335 Leu Thr Gly Ala Gly Thr Glu Gly Glu Thr Tyr Phe Asp Met Gly Ala 340 345 350 Asp Leu Gln Trp Gln Pro Ser Arg Arg Ala Leu Asp Ala Ala Gly Tyr 355 360 365 Glu Leu Pro Tyr Ile Glu Glu Val Asp Ala Gly Gln Asp Glu Glu Gly 370 375 380 Glu Tyr Leu Glu Asp 385 <210> 170 <211> 587 <212> PRT <213> Simian adenovirus 35 <400> 170 Met Glu Gln Gln Ala Pro Asp Pro Ala Ile Arg Ala Ala Leu Gln Ser 1 5 10 15 Gln Pro Ser Gly Ile Asn Ser Ser Asp Asp Trp Ser Gln Ala Met Gln 20 25 30 Arg Ile Met Ala Leu Thr Thr Arg Asn Pro Glu Ala Phe Arg Gln Gln 35 40 45 Pro Gln Ala Asn Arg Leu Ser Ala Ile Leu Glu Ala Val Val Pro Ser 50 55 60 Arg Ser Asn Pro Thr His Glu Lys Val Leu Ala Ile Val Asn Ala Leu 65 70 75 80 Val Glu Asn Lys Ala Ile Arg Pro Asp Glu Ala Gly Leu Val Tyr Asn 85 90 95 Ala Leu Leu Glu Arg Val Ala Arg Tyr Asn Ser Ser Asn Val Gln Thr 100 105 110 Asn Leu Asp Arg Met Val Thr Asp Val Arg Glu Ala Val Ser Gln Arg 115 120 125 Glu Arg Phe Gln Arg Asp Ala Asn Leu Gly Ser Leu Val Ala Leu Asn 130 135 140 Ala Phe Leu Ser Thr Gln Pro Ala Asn Val Pro Arg Gly Gln Gln Asp 145 150 155 160 Tyr Thr Asn Phe Leu Ser Ala Leu Arg Leu Met Val Ala Glu Val Pro 165 170 175 Gln Ser Glu Val Tyr Gln Ser Gly Pro Asp Tyr Phe Phe Gln Thr Ser 180 185 190 Arg Gln Gly Leu Gln Thr Val Asn Leu Ser Gln Ala Phe Lys Asn Leu 195 200 205 Asn Gly Leu Trp Gly Val Arg Ala Pro Val Gly Asp Arg Ala Thr Val 210 215 220 Ser Ser Leu Leu Thr Pro Asn Ser Arg Leu Leu Leu Leu Leu Val Ala 225 230 235 240 Pro Phe Thr Asp Ser Gly Ser Ile Asp Arg Asn Ser Tyr Leu Gly Tyr 245 250 255 Leu Leu Asn Leu Tyr Arg Glu Ala Ile Gly Gln Thr Gln Val Asp Glu 260 265 270 Gln Thr Tyr Gln Glu Ile Thr Gln Val Ser Arg Ala Leu Gly Gln Glu 275 280 285 Asp Thr Gly Ser Leu Glu Ala Thr Leu Asn Phe Leu Leu Thr Asn Arg 290 295 300 Ser Gln Lys Ile Pro Pro Gln Tyr Ala Leu Thr Ala Glu Glu Glu Arg 305 310 315 320 Ile Leu Arg Tyr Val Gln Gln Ser Val Gly Leu Phe Leu Met Gln Glu 325 330 335 Gly Ala Thr Pro Thr Ala Ala Leu Asp Met Thr Ala Arg Asn Met Glu 340 345 350 Pro Ser Met Tyr Ala Ser Asn Arg Pro Phe Ile Asn Lys Leu Leu Asp 355 360 365 Tyr Leu His Arg Ala Ala Ala Met Asn Ser Asp Tyr Phe Thr Asn Ala 370 375 380 Ile Leu Asn Pro His Trp Leu Pro Pro Pro Gly Phe Tyr Thr Gly Glu 385 390 395 400 Tyr Asp Met Pro Asp Pro Asn Asp Gly Phe Leu Trp Asp Asp Val Asp 405 410 415 Ser Ser Val Phe Ser Pro Pro Pro Gly Tyr Asn Thr Trp Lys Lys Glu 420 425 430 Gly Gly Asp Arg Arg His Ser Ser Val Ser Leu Ser Gly Ala Thr Gly 435 440 445 Ala Ala Ala Ala Val Pro Glu Ala Ala Ser Pro Phe Pro Ser Leu Pro 450 455 460 Phe Ser Leu Asn Ser Val Arg Ser Ser Glu Leu Gly Arg Ile Thr Arg 465 470 475 480 Pro Arg Leu Ile Gly Glu Glu Glu Tyr Leu Asn Asp Ser Leu Leu Arg 485 490 495 Pro Glu Arg Glu Lys Asn Phe Pro Asn Asn Gly Ile Glu Ser Leu Val 500 505 510 Asp Lys Met Asn Arg Trp Lys Thr Tyr Ala His Asp His Arg Asp Asp 515 520 525 Pro Arg Ala Leu Gly Asp Ser Arg Gly Ser Ala Thr Arg Lys Arg Gln 530 535 540 Trp His Asp Arg Gln Arg Gly Leu Val Trp Ala Asp Glu Asp Ser Ala 545 550 555 560 Asp Asp Ser Ser Val Leu Asp Leu Gly Gly Ser Gly Gly Asn Pro Phe 565 570 575 Ala His Leu Arg Pro Arg Val Gly Arg Leu Met 580 585 <210> 171 <211> 563 <212> PRT <213> Simian adenovirus 35 <400> 171 Met Met Arg Arg Thr Val Leu Gly Gly Ala Val Val Tyr Pro Glu Gly 1 5 10 15 Pro Pro Pro Ser Tyr Glu Ser Val Met Gln Gln Ala Ala Ala Ala Ala 20 25 30 Met Gln Pro Pro Leu Glu Ala Pro Phe Val Pro Pro Arg Tyr Leu Ala 35 40 45 Pro Thr Glu Gly Arg Asn Ser Ile Arg Tyr Ser Glu Leu Ala Pro Leu 50 55 60 Tyr Asp Thr Thr Arg Leu Tyr Leu Val Asp Asn Lys Ser Ala Asp Ile 65 70 75 80 Ala Ser Leu Asn Tyr Gln Asn Asp His Ser Asn Phe Leu Thr Thr Val 85 90 95 Val Gln Asn Asn Asp Phe Thr Pro Thr Glu Ala Ser Thr Gln Thr Ile 100 105 110 Asn Phe Asp Glu Arg Ser Arg Trp Gly Gly Gln Leu Lys Thr Ile Met 115 120 125 His Thr Asn Met Pro Asn Val Asn Glu Tyr Met Phe Ser Asn Lys Phe 130 135 140 Lys Ala Arg Val Met Val Ser Arg Lys Ala Pro Glu Gly Val Thr Val 145 150 155 160 Asp Asp Asn Tyr Asp His Lys Gln Asp Ile Leu Glu Tyr Glu Trp Phe 165 170 175 Glu Phe Thr Leu Pro Glu Gly Asn Phe Ser Ala Thr Met Thr Ile Asp 180 185 190 Leu Met Asn Asn Ala Ile Ile Asp Asn Tyr Leu Glu Val Gly Arg Gln 195 200 205 Asn Gly Val Leu Glu Ser Asp Ile Gly Val Lys Phe Asp Thr Arg Asn 210 215 220 Phe Arg Leu Gly Trp Asp Pro Glu Thr Lys Leu Ile Met Pro Gly Val 225 230 235 240 Tyr Thr Tyr Glu Ala Phe His Pro Asp Ile Val Leu Leu Pro Gly Cys 245 250 255 Gly Val Asp Phe Thr Glu Ser Arg Leu Ser Asn Leu Leu Gly Ile Arg 260 265 270 Lys Arg His Pro Phe Gln Glu Gly Phe Lys Ile Leu Tyr Glu Asp Leu 275 280 285 Glu Gly Gly Asn Ile Pro Ala Leu Leu Asp Val Glu Ala Tyr Glu Asn 290 295 300 Ser Lys Lys Glu Gln Glu Ala Lys Thr Glu Ala Ala Lys Ala Ala Ala 305 310 315 320 Ile Ala Lys Ala Asn Ile Val Val Ser Asp Pro Val Arg Val Ala Asn 325 330 335 Ala Glu Glu Val Arg Gly Asp Asn Tyr Thr Ala Ser Ser Val Ala Thr 340 345 350 Glu Glu Ser Leu Leu Ala Ala Val Ala Glu Thr Glu Thr Thr Glu Thr 355 360 365 Lys Leu Thr Ile Lys Pro Val Glu Lys Asp Ser Lys Ser Arg Ser Tyr 370 375 380 Asn Val Leu Glu Asp Lys Val Asn Thr Ala Tyr Arg Ser Trp Tyr Leu 385 390 395 400 Ser Tyr Asn Tyr Gly Asp Pro Glu Lys Gly Val Arg Ser Trp Thr Leu 405 410 415 Leu Thr Thr Ser Asp Val Thr Cys Gly Ala Glu Gln Val Tyr Trp Ser 420 425 430 Leu Pro Asp Met Met Gln Asp Pro Val Thr Phe Arg Ser Thr Arg Gln 435 440 445 Val Ser Asn Tyr Pro Val Val Gly Ala Glu Leu Met Pro Val Phe Ser 450 455 460 Lys Ser Phe Tyr Asn Glu Gln Ala Val Tyr Ser Gln Gln Leu Arg Gln 465 470 475 480 Ser Thr Ser Leu Thr His Val Phe Asn Arg Phe Pro Glu Asn Gln Ile 485 490 495 Leu Ile Arg Pro Pro Ala Pro Thr Ile Thr Thr Val Ser Glu Asn Val 500 505 510 Pro Ala Leu Thr Asp His Gly Thr Leu Pro Leu Arg Ser Ser Ile Arg 515 520 525 Gly Val Gln Arg Val Thr Val Thr Asp Ala Arg Arg Arg Thr Cys Pro 530 535 540 Tyr Val Tyr Lys Ala Leu Gly Ile Val Ala Pro Arg Val Leu Ser Ser 545 550 555 560 Arg Thr Phe <210> 172 <211> 192 <212> PRT <213> Simian adenovirus 35 <400> 172 Met Ser Ile Leu Ile Ser Pro Ser Asn Asn Thr Gly Trp Gly Leu Arg 1 5 10 15 Ala Pro Ser Lys Met Tyr Gly Gly Ala Arg Lys Arg Ser Thr Gln His 20 25 30 Pro Val Arg Val Arg Gly His Phe Arg Ala Pro Trp Gly Ala Leu Lys 35 40 45 Gly Arg Thr Arg Thr Arg Thr Thr Val Asp Asp Val Ile Asp Gln Val 50 55 60 Val Ala Asp Ala Arg Asn Tyr Thr Pro Thr Ala Pro Thr Ser Thr Val 65 70 75 80 Asp Ala Val Ile Asp Ser Val Val Ala Asp Ala Arg Ala Tyr Ala Arg 85 90 95 Arg Lys Ser Arg Arg Arg Arg Ile Ala Arg Arg His Arg Ala Thr Pro 100 105 110 Ala Met Arg Ala Ala Arg Ala Leu Leu Arg Arg Ala Lys Arg Val Gly 115 120 125 Arg Arg Ala Met Leu Arg Ala Ala Arg Arg Ala Ala Ser Gly Ala Ser 130 135 140 Ala Gly Arg Ser Arg Arg Arg Ala Ala Thr Ala Ala Ala Ala Ala Ile 145 150 155 160 Ala Asn Met Ala Gln Pro Arg Arg Gly Asn Val Tyr Trp Val Arg Asp 165 170 175 Ala Thr Thr Gly Gln Arg Val Pro Val Arg Thr Arg Pro Pro Arg Ser 180 185 190 <210> 173 <211> 353 <212> PRT <213> Simian adenovirus 35 <400> 173 Met Ser Lys Arg Lys Tyr Lys Glu Glu Met Leu Gln Val Ile Ala Pro 1 5 10 15 Glu Ile Tyr Gly Pro Pro Val Lys Asp Glu Lys Lys Pro Arg Lys Ile 20 25 30 Lys Arg Val Lys Lys Asp Lys Lys Glu Glu Asp Gly Asn Asp Gly Leu 35 40 45 Val Glu Phe Val Arg Glu Phe Ala Pro Arg Arg Arg Val Gln Trp Arg 50 55 60 Gly Arg Lys Val Arg Pro Val Leu Arg Pro Gly Thr Thr Val Val Phe 65 70 75 80 Thr Pro Gly Glu Arg Thr Ser Thr Ala Phe Lys Arg Ser Tyr Asp Glu 85 90 95 Val Tyr Gly Asp Asp Asp Ile Leu Glu Gln Ala Ala Asp Arg Leu Gly 100 105 110 Glu Phe Ala Tyr Gly Lys Arg Ser Arg Ser Ser Pro Lys Glu Glu Ala 115 120 125 Val Ser Ile Pro Leu Asp Asn Gly Asn Pro Thr Pro Ser Leu Lys Pro 130 135 140 Val Thr Leu Gln Gln Val Leu Pro Val Pro Pro Arg Arg Gly Asn Lys 145 150 155 160 Arg Glu Gly Glu Asp Leu Tyr Pro Thr Met Gln Leu Met Val Pro Lys 165 170 175 Arg Gln Arg Leu Glu Asp Val Leu Glu Lys Met Lys Val Asp Pro Asp 180 185 190 Ile Gln Pro Glu Val Lys Val Arg Pro Ile Lys Gln Val Ala Pro Gly 195 200 205 Leu Gly Val Gln Thr Val Asp Ile Lys Ile Pro Thr Glu Ser Met Glu 210 215 220 Val Gln Thr Glu Pro Ala Lys Pro Thr Thr Thr Ser Ile Glu Val Gln 225 230 235 240 Thr Asp Pro Trp Met Ser Ala Pro Val Thr Ala Gln Ala Ala Val Asn 245 250 255 Thr Thr Arg Arg Ser Arg Arg Lys Tyr Gly Pro Ala Ser Leu Leu Met 260 265 270 Pro Asn Tyr Ala Leu His Pro Ser Ile Ile Pro Thr Pro Gly Tyr Arg 275 280 285 Gly Thr Arg Tyr Tyr Arg Ser Arg Ser Ser Thr Ser Arg Arg Arg Arg 290 295 300 Lys Thr Pro Ala Ser Arg Ser His Arg Arg Arg Arg Arg Pro Ala Ser 305 310 315 320 Asn Leu Thr Pro Ala Ala Leu Val Arg Arg Val Tyr Arg Asp Gly Arg 325 330 335 Ala Asp Pro Leu Thr Leu Pro Arg Val Arg Tyr His Pro Ser Ile Thr 340 345 350 Thr <210> 174 <211> 76 <212> PRT <213> Simian adenovirus 35 <400> 174 Met Ala Leu Thr Cys Arg Leu Arg Val Pro Ile Thr Gly Tyr Arg Gly 1 5 10 15 Arg Asn Ser Arg Arg Arg Arg Gly Met Leu Gly Arg Gly Met Arg Arg 20 25 30 His Arg Arg Arg Arg Ala Ile Ser Lys Arg Leu Gly Gly Gly Phe Leu 35 40 45 Pro Ala Leu Ile Pro Ile Ile Ala Ala Ala Ile Gly Ala Ile Pro Gly 50 55 60 Ile Ala Ser Val Ala Val Gln Ala Ser Gln Arg His 65 70 75 <210> 175 <211> 250 <212> PRT <213> Simian adenovirus 35 <400> 175 Met Glu Asp Ile Asn Phe Ser Ser Leu Ala Pro Arg His Gly Thr Arg 1 5 10 15 Pro Tyr Met Gly Thr Trp Ser Asp Ile Gly Thr Ser Gln Leu Asn Gly 20 25 30 Gly Ala Phe Asn Trp Ser Ser Ile Trp Ser Gly Leu Lys Asn Phe Gly 35 40 45 Ser Thr Ile Lys Thr Tyr Gly Asn Lys Ala Trp Asn Ser Ser Thr Gly 50 55 60 Gln Ala Leu Arg Asn Lys Leu Lys Glu Gln Asn Phe Gln Gln Lys Val 65 70 75 80 Val Asp Gly Ile Ala Ser Gly Ile Asn Gly Val Val Asp Leu Ala Asn 85 90 95 Gln Ala Val Gln Lys Gln Ile Asn Ser Arg Leu Asp Pro Pro Pro Ser 100 105 110 Ala Pro Gly Glu Met Glu Val Glu Glu Asp Leu Pro Pro Leu Glu Lys 115 120 125 Arg Gly Asp Lys Arg Pro Arg Pro Asp Leu Glu Glu Thr Leu Val Thr 130 135 140 Arg Ser Asp Asp Pro Pro Ser Tyr Glu Glu Ala Val Lys Leu Gly Met 145 150 155 160 Pro Thr Thr Arg Pro Val Ala Pro Met Ala Thr Gly Val Met Lys Pro 165 170 175 Ser Gln Ser His Arg Pro Ala Thr Leu Asp Leu Pro Pro Pro Pro Thr 180 185 190 Ala Ala Ala Pro Ala Arg Lys Pro Val Ala Thr Pro Lys Pro Thr Thr 195 200 205 Val Gln Pro Val Ala Val Ala Arg Pro Arg Pro Gly Gly Thr Pro Arg 210 215 220 Pro Asn Ala Asn Trp Gln Ser Thr Leu Asn Ser Ile Val Gly Leu Gly 225 230 235 240 Val Gln Ser Val Lys Arg Arg Arg Cys Phe 245 250 <210> 176 <211> 956 <212> PRT <213> Simian adenovirus 35 <400> 176 Met Ala Thr Pro Ser Met Leu Pro Gln Trp Ala Tyr Met His Ile Ala 1 5 10 15 Gly Gln Asp Ala Ser Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala 20 25 30 Arg Ala Thr Asp Thr Tyr Phe Asn Leu Gly Asn Lys Phe Arg Asn Pro 35 40 45 Thr Val Ala Pro Thr His Asp Val Thr Thr Asp Arg Ser Gln Arg Leu 50 55 60 Met Leu Arg Phe Val Pro Val Asp Arg Glu Asp Asn Thr Tyr Ser Tyr 65 70 75 80 Lys Val Arg Tyr Thr Leu Ala Val Gly Asp Asn Arg Val Leu Asp Met 85 90 95 Ala Ser Thr Phe Phe Asp Ile Arg Gly Val Leu Asp Arg Gly Pro Ser 100 105 110 Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser Leu Ala Pro Lys Gly 115 120 125 Ala Pro Asn Thr Ser Gln Trp Ile Ala Glu Gly Val Lys Lys Glu Asn 130 135 140 Gly Glu Ala Asp Asn Glu Ala Ala Val Glu Glu Glu Glu Glu Glu Lys 145 150 155 160 Asn Leu Thr Thr Tyr Thr Phe Gly Asn Ala Pro Val Lys Ala Glu Gly 165 170 175 Gly Asp Ile Thr Lys Asp Lys Gly Leu Pro Ile Gly Ser Glu Ile Thr 180 185 190 Asp Gly Lys Ala Lys Pro Ile Tyr Ala Asp Lys Leu Tyr Gln Pro Glu 195 200 205 Pro Gln Val Gly Glu Glu Thr Trp Thr Asp Thr Asp Gly Thr Thr Glu 210 215 220 Lys Tyr Gly Gly Arg Ala Leu Lys Pro Glu Thr Lys Met Lys Pro Cys 225 230 235 240 Tyr Gly Ser Phe Ala Lys Pro Thr Asn Val Lys Gly Gly Gln Ala Lys 245 250 255 Gln Lys Thr Thr Glu Gln Leu Gln Asn Gln Gln Val Glu Tyr Asp Ile 260 265 270 Asp Met Asn Phe Phe Asp Gln Ala Ser Gln Lys Ala Asn Phe Ser Pro 275 280 285 Lys Ile Val Met Tyr Ala Glu Asn Val Asp Leu Glu Thr Pro Asp Thr 290 295 300 His Val Val Tyr Lys Pro Gly Thr Ser Glu Glu Ser Ser His Ala Asn 305 310 315 320 Leu Gly Gln Gln Ser Met Pro Asn Arg Pro Asn Tyr Ile Gly Phe Arg 325 330 335 Asp Asn Phe Ile Gly Leu Met Tyr Tyr Asn Ser Thr Gly Asn Met Gly 340 345 350 Val Leu Ala Gly Gln Ala Ser Gln Leu Asn Ala Val Val Asp Leu Gln 355 360 365 Asp Arg Asn Thr Glu Leu Ser Tyr Gln Leu Leu Leu Asp Ser Leu Gly 370 375 380 Asp Arg Thr Arg Tyr Phe Ser Met Trp Asn Gln Ala Val Asp Ser Tyr 385 390 395 400 Asp Pro Asp Val Arg Ile Ile Glu Asn His Gly Val Glu Asp Glu Leu 405 410 415 Pro Asn Tyr Cys Phe Pro Leu Asp Gly Val Gly Val Pro Thr Thr Ser 420 425 430 Tyr Lys Ile Ile Glu Pro Asn Gly Glu Gly Ala Asp Trp Lys Glu Pro 435 440 445 Asp Ile Asn Gly Thr Ser Glu Ile Gly Gln Gly Asn Leu Phe Ala Met 450 455 460 Glu Ile Asn Leu Gln Ala Asn Leu Trp Arg Ser Phe Leu Tyr Ser Asn 465 470 475 480 Val Ala Leu Tyr Leu Pro Asp Ser Tyr Lys Tyr Thr Pro Ala Asn Val 485 490 495 Thr Leu Pro Thr Asn Thr Asn Thr Tyr Asp Tyr Met Asn Gly Arg Val 500 505 510 Val Pro Pro Ser Leu Val Asp Thr Tyr Val Asn Ile Gly Ala Arg Trp 515 520 525 Ser Leu Asp Ala Met Asp Asn Val Asn Pro Phe Asn His His Arg Asn 530 535 540 Ala Gly Leu Arg Tyr Arg Ser Met Leu Leu Gly Asn Gly Arg Tyr Val 545 550 555 560 Pro Phe His Ile Gln Val Pro Gln Lys Phe Phe Ala Val Lys Asn Leu 565 570 575 Leu Leu Leu Pro Gly Ser Tyr Thr Tyr Glu Trp Asn Phe Arg Lys Asp 580 585 590 Val Asn Met Val Leu Gln Ser Ser Leu Gly Asn Asp Leu Arg Val Asp 595 600 605 Gly Ala Ser Ile Ser Phe Thr Ser Ile Asn Leu Tyr Ala Thr Phe Phe 610 615 620 Pro Met Ala His Asn Thr Ala Ser Thr Leu Glu Ala Met Leu Arg Asn 625 630 635 640 Asp Thr Asn Asp Gln Ser Phe Asn Asp Tyr Leu Ser Ala Ala Asn Met 645 650 655 Leu Tyr Pro Ile Pro Ala Asn Ala Thr Asn Val Pro Ile Ser Ile Pro 660 665 670 Ser Arg Asn Trp Ala Ala Phe Arg Gly Trp Ser Phe Thr Arg Leu Lys 675 680 685 Thr Lys Glu Thr Pro Ser Leu Gly Ser Gly Phe Asp Pro Tyr Phe Val 690 695 700 Tyr Ser Gly Ser Ile Pro Tyr Leu Asp Gly Thr Phe Tyr Leu Asn His 705 710 715 720 Thr Phe Lys Lys Val Ser Ile Met Phe Asp Ser Ser Val Ser Trp Pro 725 730 735 Gly Asn Asp Arg Leu Leu Thr Pro Asn Glu Phe Glu Ile Lys Arg Thr 740 745 750 Val Asp Gly Glu Gly Tyr Asn Val Ala Gln Cys Asn Met Thr Lys Asp 755 760 765 Trp Phe Leu Val Gln Met Leu Ala Asn Tyr Asn Ile Gly Tyr Gln Gly 770 775 780 Phe Tyr Val Pro Glu Gly Tyr Lys Asp Arg Met Tyr Ser Phe Phe Arg 785 790 795 800 Asn Phe Gln Pro Met Ser Arg Gln Val Val Asp Glu Ile Asn Tyr Lys 805 810 815 Asp Tyr Lys Ala Val Ala Val Pro Tyr Gln His Asn Asn Ser Gly Phe 820 825 830 Val Gly Tyr Met Ala Pro Thr Met Arg Gln Gly Gln Ala Tyr Pro Ala 835 840 845 Asn Tyr Pro Tyr Pro Leu Ile Gly Thr Thr Ala Val Thr Ser Val Thr 850 855 860 Gln Lys Lys Phe Leu Cys Asp Arg Thr Met Trp Arg Ile Pro Phe Ser 865 870 875 880 Ser Asn Phe Met Ser Met Gly Ala Leu Thr Asp Leu Gly Gln Asn Leu 885 890 895 Leu Tyr Ala Asn Ser Ala His Ala Leu Asp Met Thr Phe Glu Val Asp 900 905 910 Pro Met Asp Glu Pro Thr Leu Leu Tyr Leu Leu Phe Glu Val Phe Asp 915 920 925 Val Val Arg Val His Gln Pro His Arg Gly Val Ile Glu Ala Val Tyr 930 935 940 Leu Arg Thr Pro Phe Ser Ala Gly Asn Ala Thr Thr 945 950 955 <210> 177 <211> 828 <212> PRT <213> Simian adenovirus 35 <400> 177 Met Glu Thr Gln Pro Ser Leu Pro Thr Pro Leu Gln Ala Pro Ser His 1 5 10 15 Leu Ala Ser Ser Asp Glu Glu Glu Glu Gln Ser Leu Thr Ala Pro Pro 20 25 30 Pro Ser Pro Ala Thr Thr Thr Ser Thr Leu Glu Asp Glu Glu Val Asp 35 40 45 Ala Pro Gln Glu Ile Arg Thr Gln Asp Met Glu Asp Glu Lys Ala Glu 50 55 60 Glu Ile Glu Ala Asp Ile Glu Gln Asp Pro Gly Tyr Val Thr Pro Ala 65 70 75 80 Glu His Glu Glu Glu Leu Arg Arg Phe Leu Glu Lys Asp Asp Asp Asn 85 90 95 Arg Pro Glu Gln Gln Ala Asp Gly Asp Gln Gln Asn Val Gly Leu Gly 100 105 110 Asp His Val Val Asp Tyr Leu Thr Gly Leu Gly Gly Glu Asp Val Leu 115 120 125 Leu Lys His Leu Ala Arg Gln Ser Ile Ile Ile Lys Asp Ala Leu Leu 130 135 140 Asp Arg Ser Glu Val Pro Ile Ser Val Glu Glu Leu Ser Arg Ala Tyr 145 150 155 160 Glu Leu Asn Leu Phe Ser Pro Arg Val Pro Pro Lys Arg Gln Pro Asn 165 170 175 Gly Thr Cys Glu Pro Asn Pro Arg Leu Asn Phe Tyr Pro Ala Phe Thr 180 185 190 Val Pro Glu Val Leu Ala Thr Tyr His Ile Phe Phe Lys Asn Gln Lys 195 200 205 Ile Pro Ile Ser Cys Arg Ala Asn Arg Thr Arg Ala Asp Ala Leu Leu 210 215 220 Asn Leu Gly Pro Gly Ala Cys Leu Pro Asp Ile Thr Ser Leu Glu Glu 225 230 235 240 Val Pro Lys Ile Phe Glu Gly Leu Gly Ser Asp Glu Thr Arg Ala Ala 245 250 255 Asn Ala Leu Gln Gln Gly Glu Asn Gly Ile Asp Glu His His Ser Ala 260 265 270 Leu Val Glu Leu Glu Gly Asp Asn Ala Arg Leu Ala Val Leu Lys Arg 275 280 285 Ser Ile Glu Val Thr His Phe Ala Tyr Pro Ala Val Asn Leu Pro Pro 290 295 300 Lys Val Met Ser Ala Val Met Asp Gln Ile Leu Ile Lys Arg Ala Ser 305 310 315 320 Pro Leu Ser Glu Asn Met Gln Asp Pro Asp Ala Ser Asp Glu Gly Lys 325 330 335 Pro Val Val Ser Asp Glu Gln Leu Ser Arg Trp Leu Gly Thr Asn Ser 340 345 350 Pro Arg Asp Leu Glu Glu Arg Arg Lys Leu Met Met Ala Val Val Leu 355 360 365 Val Thr Val Glu Met Glu Cys Leu Arg Arg Phe Phe Thr Asp Pro Glu 370 375 380 Thr Leu Arg Lys Leu Glu Glu Asn Leu His Tyr Thr Phe Arg His Gly 385 390 395 400 Phe Val Arg Gln Ala Cys Lys Ile Ser Asn Val Glu Leu Thr Asn Leu 405 410 415 Val Ser Tyr Met Gly Ile Leu His Glu Asn Arg Leu Gly Gln Ser Val 420 425 430 Leu His Thr Thr Leu Lys Gly Glu Ala Arg Arg Asp Tyr Ile Arg Asp 435 440 445 Thr Val Tyr Leu Tyr Leu Cys His Thr Trp Gln Thr Gly Met Gly Val 450 455 460 Trp Gln Gln Cys Leu Glu Glu Gln Asn Leu Lys Glu Leu Asp Lys Leu 465 470 475 480 Leu Gln Arg Ser Leu Lys Thr Leu Trp Thr Gly Phe Asp Glu Arg Thr 485 490 495 Val Ala Ser Asp Leu Ala Asp Leu Ile Phe Pro Glu Arg Leu Arg Thr 500 505 510 Thr Leu Arg Asn Gly Leu Pro Asp Phe Met Asn Gln Ser Met Ile Asn 515 520 525 Asn Phe Arg Ser Phe Ile Leu Glu Arg Ser Gly Ile Leu Pro Ala Thr 530 535 540 Cys Cys Ala Leu Pro Ser Asp Phe Val Pro Leu Thr Tyr Arg Glu Cys 545 550 555 560 Pro Pro Pro Leu Trp Ser His Cys Tyr Leu Phe Arg Leu Ala Asn Tyr 565 570 575 Leu Ser Tyr His Ser Asp Val Ile Glu Asp Val Ser Gly Asp Gly Leu 580 585 590 Leu Glu Cys His Cys Arg Cys Asn Leu Cys Ser Pro His Arg Ser Leu 595 600 605 Val Cys Asn Pro Gln Leu Leu Ser Glu Thr Gln Ile Ile Gly Thr Phe 610 615 620 Glu Leu Gln Gly Pro Ser Ser Glu Gly Glu Gly Ser Ser Pro Gly Gln 625 630 635 640 Ser Leu Lys Leu Thr Pro Gly Leu Trp Thr Ser Ala Tyr Leu Arg Lys 645 650 655 Phe Ser Pro Glu Asp Tyr His Pro Tyr Glu Ile Arg Phe Tyr Glu Asp 660 665 670 Gln Ser Gln Pro Pro Lys Ala Glu Leu Ser Ala Cys Val Ile Thr Gln 675 680 685 Gly Ala Ile Leu Ala Gln Leu Gln Ala Ile Gln Lys Ser Arg Gln Glu 690 695 700 Phe Leu Leu Lys Lys Gly Asn Gly Val Tyr Leu Asp Pro Gln Thr Gly 705 710 715 720 Glu Glu Leu Asn Thr Arg Phe Ser Gln Asp Val Ser Ala Pro Arg Lys 725 730 735 Gln Glu Val Glu Ser Ala Ala Ala Ala Pro Arg Gly Tyr Gly Gly Arg 740 745 750 Leu Gly Gln Ser Asp Arg Gly Asp Gly Arg Leu Gly Gln Pro Gly Arg 755 760 765 Gly Gly Gly Gly Gln Pro Gly Gly Arg Gln Phe Gly Gly Gly Arg Arg 770 775 780 Gly Gly Arg Gly Gly Gly Arg Ser Asn Arg Arg Gln Thr Val Val Leu 785 790 795 800 Gly Gly Gly Asp Lys Gln Gly His Arg Gln His His Ser Tyr His Leu 805 810 815 Arg Ser Gly Ser Gly Gly Pro Ala Pro Ser Gln Gln 820 825 <210> 178 <211> 227 <212> PRT <213> Simian adenovirus 35 <400> 178 Met Ser Lys Glu Ile Pro Thr Pro Tyr Met Trp Ser Tyr Gln Pro Gln 1 5 10 15 Met Gly Leu Ala Ala Gly Ala Ser Gln Asp Tyr Ser Thr Arg Met Asn 20 25 30 Trp Leu Ser Ala Gly Pro Ser Met Ile Ser Arg Val Asn Asp Ile Arg 35 40 45 Ala Tyr Arg Asn Gln Leu Leu Leu Glu Gln Ser Ala Leu Thr Ala Thr 50 55 60 Pro Arg Gln His Leu Asn Pro Arg Asn Trp Pro Ala Ala Leu Val Tyr 65 70 75 80 Gln Glu Thr Pro Ala Pro Thr Thr Val Leu Leu Pro Arg Asp Ala Gln 85 90 95 Ala Glu Val Gln Met Thr Asn Ala Gly Val Gln Leu Ala Gly Gly Ser 100 105 110 Ala Leu Cys Arg His Arg Pro Gln Gln Ser Ile Lys Arg Leu Val Ile 115 120 125 Arg Gly Arg Gly Ile Gln Leu Asn Asp Glu Ser Val Ser Ser Ser Leu 130 135 140 Gly Leu Arg Pro Asp Gly Val Phe Gln Ile Ala Gly Cys Gly Arg Ser 145 150 155 160 Ser Phe Thr Pro Arg Gln Ala Val Leu Thr Leu Glu Ser Ser Ser Ser 165 170 175 Gln Pro Arg Ser Gly Gly Ile Gly Thr Leu Gln Phe Val Glu Glu Phe 180 185 190 Thr Pro Ser Val Tyr Phe Asn Pro Phe Ser Gly Ser Pro Gly His Tyr 195 200 205 Pro Asp Glu Phe Ile Pro Asn Phe Asp Ala Ile Ser Glu Ser Val Asp 210 215 220 Gly Tyr Asp 225 <210> 179 <211> 106 <212> PRT <213> Simian adenovirus 35 <400> 179 Met Ser Asn Gly Gly Ala Ala Glu Leu Ala Arg Leu Arg His Leu Asp 1 5 10 15 His Cys Arg Arg Phe Arg Cys Phe Ala Arg Glu Leu Thr Glu Phe Ile 20 25 30 Tyr Phe Glu Ile Pro Glu Glu His Pro Gln Gly Pro Ala His Gly Val 35 40 45 Arg Ile Thr Ile Glu Gly Gly Ile Asp Ser Arg Leu His Arg Ile Phe 50 55 60 Cys Gln Arg Pro Val Leu Ile Glu Arg Asp Gln Gly Asn Thr Thr Val 65 70 75 80 Ser Ile Tyr Cys Ile Cys Asn His Pro Gly Leu His Glu Ser Leu Cys 85 90 95 Cys Leu Ile Cys Ala Glu Phe Asn Lys Asn 100 105 <210> 180 <211> 172 <212> PRT <213> Simian adenovirus 35 <400> 180 Met Gly Ala Leu Leu Val Ala Leu Ala Leu Leu Ser Leu Leu Asp Leu 1 5 10 15 Gly Ser Thr Met Leu Val Gln Pro Val Leu Phe Asp Pro Cys Leu Asn 20 25 30 Phe Asp Pro Asp Asn Cys Thr Leu Thr Phe Ala Pro Glu Ala Gly Arg 35 40 45 Cys Gly Val Leu Ile Arg Cys Gly Arg Glu Cys Ser Pro Ile Glu Ile 50 55 60 His His Asn Asn Lys Ile Trp Asn Asn Thr Leu Phe Thr Thr Trp Gln 65 70 75 80 Pro Gly Asp Pro Glu Trp Tyr Thr Val Ser Val Arg Gly Pro Asp Gly 85 90 95 Ser Ile Arg Thr Ala Asn Asn Thr Phe Ile Phe Ala Glu Met Cys Asp 100 105 110 Leu Thr Met Phe Met Ser Lys Gln Tyr Asn Leu Trp Pro Pro Ser Lys 115 120 125 Glu Asn Ile Val Ala Phe Ser Ile Ala Tyr Phe Leu Cys Thr Cys Leu 130 135 140 Ile Thr Ala Ile Leu Cys Ile Cys Ile His Leu Leu Ile Cys His Arg 145 150 155 160 His Arg Asn Ser Asn Glu Glu Lys Glu Lys Met Pro 165 170 <210> 181 <211> 146 <212> PRT <213> Simian adenovirus 35 <400> 181 Met Ser Val Gly Gly Glu Tyr Asp Ser Gly Trp Phe Ile Arg Pro Cys 1 5 10 15 Asp Gln Pro Gly Asn Lys Phe Phe Cys Asn Gly Arg Asp Leu Thr Ile 20 25 30 Ile Asn Ile Thr Val Asn Asp Gln Gly Phe Tyr Tyr Gly Thr Asn Tyr 35 40 45 Lys Asn Asn Leu Asp Tyr Asn Ile Ile Val Val Pro Ala Thr Thr Pro 50 55 60 Ala Pro Arg Lys Thr Thr Phe Phe Ser Ser Ser Ala Ser Ile Ser Lys 65 70 75 80 Thr Ala Ser Ala Ser Phe Lys Lys Phe Ala Leu Arg Asn Ser Thr Thr 85 90 95 Ser Ser Thr Ser Asn Met Ser Lys Ser Val Ile Gly Ile Ala Ala Ala 100 105 110 Ala Ile Val Gly Leu Met Ile Ile Ile Leu Cys Ile Ile Tyr Tyr Ala 115 120 125 Cys Cys Tyr Arg Lys His Glu Gln Lys Ser Asp Pro Leu Leu Asn Phe 130 135 140 Asp Ile 145 <210> 182 <211> 100 <212> PRT <213> Simian adenovirus 35 <400> 182 Met Lys Lys Leu Ser Ile Leu Ala Phe Ile Leu Phe Glu Thr Phe Thr 1 5 10 15 Asn Val Gln Thr Thr Leu Ser His Asp Ile Glu Asn His Thr Thr Ser 20 25 30 Tyr Val Pro Thr Asn Ile Thr Thr His Pro Lys His Ala Met Gln Leu 35 40 45 Glu Ile Thr Met Leu Ile Val Val Val Ile Leu Ile Leu Ala Ile Ile 50 55 60 Phe Tyr Phe Thr Leu Cys Arg Gln Ile Pro Asn Ile His Lys Asn Ser 65 70 75 80 Lys Arg Arg Pro Ile Tyr Cys Pro Val Ile Ser Arg Pro His Met Thr 85 90 95 Leu Asn Glu Ile 100 <210> 183 <211> 91 <212> PRT <213> Simian adenovirus 35 <400> 183 Met Ile Pro Arg Asn Phe Phe Phe Thr Ile Leu Ile Cys Ala Phe Asn 1 5 10 15 Val Cys Ala Thr Phe Thr Ala Val Ala Thr Ala Thr Pro Asp Cys Ile 20 25 30 Gly Pro Phe Ala Ser Tyr Thr Leu Phe Ala Phe Val Ala Cys Thr Cys 35 40 45 Val Cys Ser Val Val Cys Leu Val Ile Asn Phe Phe Gln Leu Val Asp 50 55 60 Trp Ile Phe Val Arg Leu Ala Tyr Leu Arg His His Pro Glu Tyr Arg 65 70 75 80 Asn Gln His Val Ala Ala Leu Leu Arg Leu Ile 85 90 <210> 184 <211> 135 <212> PRT <213> Simian adenovirus 35 <400> 184 Met Thr Asp Pro Leu Ala Ala Ser Thr Ala Ala Glu Glu Leu Leu Asp 1 5 10 15 Met Asp Gly Arg Ala Ser Glu Gln Arg Leu Ala Arg Leu Arg Ile Arg 20 25 30 Gln Gln Gln Glu Arg Ala Ala Lys Glu Leu Arg Asp Ala Ile Glu Ile 35 40 45 His Gln Cys Lys Lys Gly Ile Phe Cys Leu Val Lys Gln Ala Lys Ile 50 55 60 Ser Tyr Glu Ile Thr Asn Thr Asp His Arg Leu Ser Tyr Glu Leu Gly 65 70 75 80 Pro Gln Arg Gln Lys Phe Thr Cys Met Val Gly Ile Asn Pro Ile Ile 85 90 95 Ile Thr Gln Gln Ala Gly Asp Thr Lys Gly Cys Ile His Cys Ser Cys 100 105 110 Ser Ser Thr Glu Cys Ile Tyr Thr Leu Leu Lys Thr Leu Cys Gly Leu 115 120 125 Arg Asp Leu Ile Pro Met Asn 130 135 <210> 185 <211> 353 <212> PRT <213> Simian adenovirus 35 <400> 185 Met Ala Lys Arg Ala Arg Leu Ser Ser Ser Phe Asn Pro Val Tyr Pro 1 5 10 15 Tyr Glu Asp Glu Ser Ser Ser Gln His Pro Phe Ile Asn Pro Gly Phe 20 25 30 Ile Ser Ser Asn Gly Phe Thr Gln Ser Pro Asp Gly Val Leu Thr Leu 35 40 45 Lys Cys Val Asn Pro Leu Thr Thr Ala Ser Gly Pro Leu Gln Leu Lys 50 55 60 Val Gly Ser Ser Leu Thr Val Asp Thr Ile Asp Gly Ser Leu Glu Glu 65 70 75 80 Asn Ile Thr Ala Ala Ala Pro Leu Thr Lys Thr Asn His Ser Ile Gly 85 90 95 Leu Ser Ile Gly Ser Gly Leu Gln Thr Lys Asp Asp Lys Leu Cys Leu 100 105 110 Ser Leu Gly Asp Gly Leu Val Thr Lys Asp Asp Lys Leu Cys Leu Ser 115 120 125 Leu Gly Asp Gly Leu Ile Thr Lys Asp Asp Thr Leu Cys Ala Lys Leu 130 135 140 Gly His Gly Leu Val Phe Asp Ser Ser Asn Ala Ile Thr Ile Glu Asn 145 150 155 160 Asn Thr Leu Trp Thr Gly Ala Lys Pro Ser Ala Asn Cys Val Ile Lys 165 170 175 Glu Gly Glu Asp Ser Pro Asp Cys Lys Leu Thr Leu Val Leu Val Lys 180 185 190 Asn Gly Gly Leu Ile Asn Gly Tyr Ile Thr Leu Met Gly Asp Ser Glu 195 200 205 Tyr Thr Asn Thr Leu Phe Lys Asn Lys Gln Val Thr Ile Asp Val Asn 210 215 220 Leu Ala Phe Asp Asn Thr Gly Gln Ile Ile Thr Tyr Leu Ser Ser Leu 225 230 235 240 Lys Ser Asn Leu Asn Phe Lys Asp Asn Gln Asn Met Ala Thr Gly Thr 245 250 255 Ile Thr Ser Ala Lys Gly Phe Met Pro Ser Thr Thr Ala Tyr Pro Phe 260 265 270 Ile Thr Tyr Ala Thr Gln Ser Leu Asn Glu Asp Tyr Ile Tyr Gly Glu 275 280 285 Cys Tyr Tyr Lys Ser Thr Asn Gly Thr Leu Phe Pro Leu Lys Val Thr 290 295 300 Val Thr Leu Asn Arg Arg Met Ser Ala Ser Gly Met Ala Tyr Ala Met 305 310 315 320 Asn Phe Ser Trp Ser Leu Asn Ala Glu Glu Ala Pro Glu Thr Thr Glu 325 330 335 Val Thr Leu Ile Thr Ser Pro Phe Phe Phe Ser Tyr Ile Arg Glu Asp 340 345 350 Asp <210> 186 <211> 550 <212> DNA <213> Simian adenovirus 35 <220> <221> CDS <222> (6)..(548) <223> label=E1b\19K <400> 186 catcc atg gag gtt tgg gcc atc ttg gaa gat ctt agg cag act agg caa 50 Met Glu Val Trp Ala Ile Leu Glu Asp Leu Arg Gln Thr Arg Gln 1 5 10 15 ctg cta gaa aac gcc tcg gac gga gtc tct ggt ctt tgg aga ttc tgg 98 Leu Leu Glu Asn Ala Ser Asp Gly Val Ser Gly Leu Trp Arg Phe Trp 20 25 30 ttc ggt ggt gat ctg gct aga cta gtc ttt aga ata aaa cag gat tac 146 Phe Gly Gly Asp Leu Ala Arg Leu Val Phe Arg Ile Lys Gln Asp Tyr 35 40 45 agg caa gaa ttt gaa aag tta ttg gac gac tgt tca gga ctt ttt gaa 194 Arg Gln Glu Phe Glu Lys Leu Leu Asp Asp Cys Ser Gly Leu Phe Glu 50 55 60 gct ctt aac ttg ggc cac cag gct cat ttt aag gag aag gtt tta tca 242 Ala Leu Asn Leu Gly His Gln Ala His Phe Lys Glu Lys Val Leu Ser 65 70 75 gtt ttg gat ttt tct acc cct ggt aga act gct gct gct gta gct ttc 290 Val Leu Asp Phe Ser Thr Pro Gly Arg Thr Ala Ala Ala Val Ala Phe 80 85 90 95 ctt aca ttc ata ttt gat aaa tgg atc cca cag acc cac ttc agc aag 338 Leu Thr Phe Ile Phe Asp Lys Trp Ile Pro Gln Thr His Phe Ser Lys 100 105 110 gga tac gtt ttg gat ttc ata gca gca gct ttg tgg aga aca tgg aag 386 Gly Tyr Val Leu Asp Phe Ile Ala Ala Ala Leu Trp Arg Thr Trp Lys 115 120 125 gct cgc agg atg agg aca atc tta gat tac tgg cca gta cag cct ctg 434 Ala Arg Arg Met Arg Thr Ile Leu Asp Tyr Trp Pro Val Gln Pro Leu 130 135 140 ggc gta gca ggg atc ctg aga cac cca ccg acc atg cca gcg gtt ttg 482 Gly Val Ala Gly Ile Leu Arg His Pro Pro Thr Met Pro Ala Val Leu 145 150 155 gag gag gag cac caa gag gac aat ccg aga gtc ggc ctg gac cct ccg 530 Glu Glu Glu His Gln Glu Asp Asn Pro Arg Val Gly Leu Asp Pro Pro 160 165 170 175 gtg gag gag gcg gag gag ta 550 Val Glu Glu Ala Glu Glu 180 <210> 187 <211> 181 <212> PRT <213> Simian adenovirus 35 <400> 187 Met Glu Val Trp Ala Ile Leu Glu Asp Leu Arg Gln Thr Arg Gln Leu 1 5 10 15 Leu Glu Asn Ala Ser Asp Gly Val Ser Gly Leu Trp Arg Phe Trp Phe 20 25 30 Gly Gly Asp Leu Ala Arg Leu Val Phe Arg Ile Lys Gln Asp Tyr Arg 35 40 45 Gln Glu Phe Glu Lys Leu Leu Asp Asp Cys Ser Gly Leu Phe Glu Ala 50 55 60 Leu Asn Leu Gly His Gln Ala His Phe Lys Glu Lys Val Leu Ser Val 65 70 75 80 Leu Asp Phe Ser Thr Pro Gly Arg Thr Ala Ala Ala Val Ala Phe Leu 85 90 95 Thr Phe Ile Phe Asp Lys Trp Ile Pro Gln Thr His Phe Ser Lys Gly 100 105 110 Tyr Val Leu Asp Phe Ile Ala Ala Ala Leu Trp Arg Thr Trp Lys Ala 115 120 125 Arg Arg Met Arg Thr Ile Leu Asp Tyr Trp Pro Val Gln Pro Leu Gly 130 135 140 Val Ala Gly Ile Leu Arg His Pro Pro Thr Met Pro Ala Val Leu Glu 145 150 155 160 Glu Glu His Gln Glu Asp Asn Pro Arg Val Gly Leu Asp Pro Pro Val 165 170 175 Glu Glu Ala Glu Glu 180 <210> 188 <211> 9670 <212> DNA <213> Simian adenovirus 35 <220> <221> CDS <222> (3)..(611) <223> label=protease <220> <221> CDS <222> (4632)..(5225) <223> label=22K <220> <221> CDS <222> (6531)..(6968) <223> label=E3\CR1\alpha <220> <221> CDS <222> (7449)..(8042) <223> label=E3\CR1\beta <220> <221> CDS <222> (9231)..(9665) <223> label=E3\RID\beta <400> 188 gg atg agc cca ccc tgc ttt atc ttc ttt ttg aag tat tcg acg tgg 47 Met Ser Pro Pro Cys Phe Ile Phe Phe Leu Lys Tyr Ser Thr Trp 1 5 10 15 tca gag tgc acc aac cac acc gcg gcg tca tcg agg ccg tct acc tgc 95 Ser Glu Cys Thr Asn His Thr Ala Ala Ser Ser Arg Pro Ser Thr Cys 20 25 30 gca cac cgt tct cgg ctg gta acg cca cca cat aag aaa cct gct tct 143 Ala His Arg Ser Arg Leu Val Thr Pro Pro His Lys Lys Pro Ala Ser 35 40 45 tgc aag gtg cag cca tgg cct gcg ggt ccg gaa acg gct cca gcg agc 191 Cys Lys Val Gln Pro Trp Pro Ala Gly Pro Glu Thr Ala Pro Ala Ser 50 55 60 aag agc tca gag cca tcg tcc gag acc ttg gct gtg gac cct act tcc 239 Lys Ser Ser Glu Pro Ser Ser Glu Thr Leu Ala Val Asp Pro Thr Ser 65 70 75 tgg gaa cct ttg aca aac gct tcc cgg ggt tta tgg ctc caa aca agc 287 Trp Glu Pro Leu Thr Asn Ala Ser Arg Gly Leu Trp Leu Gln Thr Ser 80 85 90 95 tgg cct gcg cca ttg tca aca cag ccg gtc gcg aga cgg ggg gag agc 335 Trp Pro Ala Pro Leu Ser Thr Gln Pro Val Ala Arg Arg Gly Glu Ser 100 105 110 act ggt tgg ctt ttg gtt gga acc cgc gct cca aca cat gct acc ttt 383 Thr Gly Trp Leu Leu Val Gly Thr Arg Ala Pro Thr His Ala Thr Phe 115 120 125 ttg atc cgt ttg gat tct cgg atg acc gtc tca agc aga tct acc agt 431 Leu Ile Arg Leu Asp Ser Arg Met Thr Val Ser Ser Arg Ser Thr Ser 130 135 140 ttg aat acg agg ggt tac tgc gcc gca gcg ccc ttg cta cta agg atc 479 Leu Asn Thr Arg Gly Tyr Cys Ala Ala Ala Pro Leu Leu Leu Arg Ile 145 150 155 gct gca tta cct tgg aaa agt cca ccc aaa ccg tgc agg gtc cgc gct 527 Ala Ala Leu Pro Trp Lys Ser Pro Pro Lys Pro Cys Arg Val Arg Ala 160 165 170 175 ccg ccg ctt gtg gac ttt ttt gct gca tgt ttc tcc atg cct ttg tac 575 Pro Pro Leu Val Asp Phe Phe Ala Ala Cys Phe Ser Met Pro Leu Tyr 180 185 190 act ggc cag acc gcc cca tgg acg gta acc cca cca tgaagttgct 621 Thr Gly Gln Thr Ala Pro Trp Thr Val Thr Pro Pro 195 200 tacgggagtg cccaacagca tgctccagtc accccaagtc cagcccaccc tgcgcaggaa 681 ccaggaggcg ctctaccatt tcctcaacac acattcatct tactttcgtt ctcaccgcgc 741 acgtatcgaa agggctactg cgttcgatcg tatgggatat taataagtca tgtaaaaccg 801 tgttcaataa acagaacttt attttttaca tgcactggtg gtttctcatt catttattca 861 ctcagaagtc gaaggggttt tggcgggaat cagagtgacc cgcgggcagg gatacgttgc 921 ggaactggaa ctgagcctgc cacttgaatt cggggatcac cagcttggga actggcaggt 981 caggcaggat gtcgctccac agcttcctgg tcagttgcag ggctcccaac aggtcaggag 1041 ctgaaatctt gaaatcgcaa ttgggacccg tgctctgagc gcgggagttg cgatacacag 1101 ggttgcaaca ctggaacacc atcagcgacg ggtatttcac actcgccagc acagtgggat 1161 cggtgataat tcccacatcc aggtcttcgg cattggccat gctaaagggg gtcatcttgc 1221 atgtctgtct gcccatagcc ggtacccagc ctggcttgtg gttgcaatcg cagcgcagag 1281 ggatcagcat catcttggcc tggtcggatc tcataccggg atacacagct ttcatgaaag 1341 cttcatattg cttgaaagcc tgttgggcct tgctaccctc agtgtagaac atcccacaag 1401 acttgctaga gaactggtta gcagcacatc cggcatcatt cacacaacag cgagcgtcgt 1461 tgttggctat ttgcaccaca ctcctgcccc agcggttctg ggtgatcttg gttcgctcag 1521 ggttctcctt cagcgcccgt tgaccgtttt cgcttgccac atccatttct atgatatgtt 1581 ccttctgaat catgatgttg ccatgcaaac acttcagctt gccttcataa tcattacatc 1641 catgtgacca cagcgcgcat cccgtacact cccagttatt gtgagcgatc tcagaatagg 1701 aatgcaccaa cccctgcagg aatcttccca tcatggttga gagggtcttg ttactggtga 1761 aagtcagcgg gacgcctcga tgctcctcgt tcacatactg gtggcaaatt cgcttgtact 1821 gttcatgctg ctctggcata agcttgaaag aggttcttag gtcattctcc agcctgtact 1881 tctccatcag cacagccatt acttccatgc ccttttccca ggcagaaacc aggggtaggc 1941 tcatggaatt tctaacagaa atagcagcta ctttagccag agggtcatcc ttgtcaatct 2001 tctcaacact tcttttgcca tccttctcag tgatgcgcac gggtgggtag ctgaagccca 2061 cggccaccag ctccgcctct tctctttctt cttcgctgtc ctgactgatg tcttgtaaag 2121 ggacatgctt ggtcttcctg ggcttctttt tggggggtat tggcggaggg ctgctgctcc 2181 gctccggaga catggaggac cgcgaagttt cgctcaccag taccacctgg ctctcggtag 2241 aagaaccgga ccccacacgg cggtaggtgt tcctcttcgg gggcagaggt ggaggtgact 2301 gcgatgggct gcggtccggc ctgggaggcg gatgactggc agagcccctt ccgcgttcgg 2361 gggtgtgctc ccggtggcgg tcgcttgact gatttcctcc gcggctggcc attgtgttct 2421 cctaggcaga gaaacaacag acatggagac tcagccatcg ctgccaacac cgctgcaagc 2481 accatcacac ctcgcctcca gcgatgagga ggaggaacaa agcttaaccg ccccaccacc 2541 cagtcccgcc accaccacct ctaccctcga ggatgaggag gtcgacgcac cccaggagat 2601 acggacgcag gatatggagg atgagaaagc ggaagagatt gaggcagata tcgagcagga 2661 cccaggctat gtgacaccgg ccgagcacga ggaagagctg agacgctttc tagagaaaga 2721 tgatgacaac cgtccagaac agcaagcaga tggcgatcag cagaatgttg ggctcgggga 2781 tcatgttgtc gactacctca ccggccttgg tggggaggac gtgctcctca aacacctagc 2841 aaggcagtcg atcataatca aagatgcact gcttgatcgc agcgaagtgc ccatcagtgt 2901 ggaagagctc agccgcgcct acgagctcaa cctgttctcg cctcgggtac cccccaagcg 2961 tcagccaaac ggcacctgcg agcccaaccc tcgcctcaac ttctatcccg cattcaccgt 3021 ccccgaggtg ctggctacct accacatatt tttcaaaaac caaaaaattc caatttcctg 3081 ccgcgccaac cgaactcgcg ccgatgccct gctcaacttg ggacctggcg cttgcttacc 3141 tgatataact tccttggaag aggtcccaaa gatcttcgaa ggtctgggca gtgatgagac 3201 tcgggccgca aatgctctgc aacagggaga gaatggcatc gatgaacatc acagcgctct 3261 ggtggagttg gagggcgata atgcccgact agcagtactc aagcgcagta tcgaggtgac 3321 ccattttgca taccccgctg tcaacctgcc tcccaaagtc atgagcgctg tcatggatca 3381 gatactcatt aaacgcgcaa gtcccctttc agaaaacatg caggatccag acgcctcgga 3441 tgagggcaag ccagtggtca gtgatgaaca gctatctcgc tggctgggca ccaactcccc 3501 acgagacttg gaagagcggc gcaagctcat gatggccgtg gtgctagtta ctgtggaaat 3561 ggagtgtctt cgccgcttct tcactgaccc cgagacactg cgcaagctcg aggagaacct 3621 acactacact tttagacatg gatttgtgag acaggcatgc aagatctcca acgtggagct 3681 taccaacctg gtttcctaca tgggcatttt gcatgaaaac agactcggac agagcgtgct 3741 gcacaccacc ctgaaggggg aagcccgtcg cgactacatc cgcgacactg tctacctcta 3801 cctctgccat acctggcaga ctggtatggg tgtgtggcag cagtgtttgg aagaacaaaa 3861 cctgaaagaa ctagacaagc tcttacagag atccctcaaa accttgtgga cgggttttga 3921 cgagcgcaca gtcgcctctg atctggcaga tctcatcttc ccagagcgtc tcaggactac 3981 tctgcgcaac gggctgcctg acttcatgaa ccagagcatg attaacaact ttcgctcttt 4041 catcctggaa cgctccggta tcctgcccgc cacctgctgt gcgctaccat ccgactttgt 4101 gcctctgacc taccgcgagt gccccccacc gctatggagc cactgctacc tgttccgcct 4161 ggccaactac ctatcatacc actcggatgt gatcgaggat gtgagcggag atggcctgct 4221 tgagtgccac tgccgctgta atctctgctc accacatcgc tccctcgtct gtaaccccca 4281 gttgcttagc gaaacccaaa ttataggcac cttcgaattg cagggtccca gcagcgaagg 4341 cgaggggtct tctcctgggc aaagtttgaa actgaccccg ggactgtgga cctccgccta 4401 cctgcgcaag ttctcccccg aggactacca cccctatgag atcaggttct atgaagacca 4461 atcacagccg cccaaagctg agctctcagc gtgcgtcatc acccaggggg caattttggc 4521 ccaattgcaa gccatccaaa aatcccgcca agaatttttg ctgaaaaagg gtaacggagt 4581 ctacctcgac ccccagactg gtgaggagct caacacaagg ttctctcagg atg tct 4637 Met Ser 205 cag cgc cga gga aac aag aag ttg aaa gtg cag ctg ccg ccc cca gag 4685 Gln Arg Arg Gly Asn Lys Lys Leu Lys Val Gln Leu Pro Pro Pro Glu 210 215 220 gat atg gag gaa gac tgg gac agt cag aca gag gag atg gaa gat tgg 4733 Asp Met Glu Glu Asp Trp Asp Ser Gln Thr Glu Glu Met Glu Asp Trp 225 230 235 gac agc cag gca gag gag gag gag gac agc ctg gag gaa gac agt ttg 4781 Asp Ser Gln Ala Glu Glu Glu Glu Asp Ser Leu Glu Glu Asp Ser Leu 240 245 250 gag gag gaa gac gag gag gca gag gag gtg gaa gaa gca acc gcc gcc 4829 Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu Ala Thr Ala Ala 255 260 265 aaa cag ttg tcc tcg gcg gcg gag aca agc aag gcc aca gac aac acc 4877 Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Ala Thr Asp Asn Thr 270 275 280 285 aca gct acc atc tcc gtt ccg ggt cgg ggg gtc cag cac cgt ccc aac 4925 Thr Ala Thr Ile Ser Val Pro Gly Arg Gly Val Gln His Arg Pro Asn 290 295 300 agt aga tgg gat gag acc ggg cga ctc ccg aat gcg acc acc gct tct 4973 Ser Arg Trp Asp Glu Thr Gly Arg Leu Pro Asn Ala Thr Thr Ala Ser 305 310 315 aag act ggt aag aag gag cgg cag gga tac aag tcc tgg cgg ggg cat 5021 Lys Thr Gly Lys Lys Glu Arg Gln Gly Tyr Lys Ser Trp Arg Gly His 320 325 330 aag aac gct atc ata tcc tgc ttg cat gaa tgc ggg ggc aac ata tcc 5069 Lys Asn Ala Ile Ile Ser Cys Leu His Glu Cys Gly Gly Asn Ile Ser 335 340 345 ttc acc cgc cgc tac ctg ctc ttc cac cac ggg gtg aac ttc ccc cgc 5117 Phe Thr Arg Arg Tyr Leu Leu Phe His His Gly Val Asn Phe Pro Arg 350 355 360 365 aat gtc ttg cat tac tac cgt cac ctc cac agc ccc tac tac agc cag 5165 Asn Val Leu His Tyr Tyr Arg His Leu His Ser Pro Tyr Tyr Ser Gln 370 375 380 caa gcc tcg gca gaa aaa gac aac agc agc aag aac ctc cag cag aaa 5213 Gln Ala Ser Ala Glu Lys Asp Asn Ser Ser Lys Asn Leu Gln Gln Lys 385 390 395 acc agc agc agt tagaacaccc acagcaggtg caacaggagg aggactgaga 5265 Thr Ser Ser Ser 400 atcacagcga acgagccagc gcagacccga gagctgagaa accggatttt tccaaccctc 5325 tatgccatct tccaacagag tcgggggcaa gagcaggaac tgaaagtaaa aaaccgatct 5385 ttgcgctcgc tcacccgaag ttgtttgtat cacaagagcg aagaccaact tcagcgcact 5445 ctcgaggacg ccgaggctct cttcaacaag tactgcgcgc tcactcttaa agagtagccc 5505 gcgcccgcgc tagctcgaaa aaaggcggga attacgtcac ccattggcgc ctgtcctttg 5565 ccctcgtcat gagtaaagaa attcccacgc cttacatgtg gagttatcaa ccccaaatgg 5625 gactggcagc aggcgcctcc caggactact ccacccgtat gaattggctc agcgccggtc 5685 cctcgatgat ctcacgggtt aatgatatac gagcttatcg aaaccaatta ctcctagaac 5745 agtcagcact taccgccaca cccagacaac accttaatcc ccggaattgg cccgccgccc 5805 tggtgtacca ggaaaccccc gctcccacca ccgtcctact tcctcgagac gcccaggccg 5865 aagttcagat gactaacgca ggtgtacagc tggctggcgg ttccgccctg tgtcgtcacc 5925 ggcctcaaca gagtataaaa cgcctggtga tcagaggccg aggtatccag ctcaacgacg 5985 agtcggtgag ctcttcgctt ggtctacgac cagacggagt cttccaaatt gccggctgcg 6045 ggagatcttc cttcactcct cgtcaggctg tactgacttt ggagagttcg tcatcgcagc 6105 cccgctcggg tggcatcggg actctccaat ttgtggagga gtttactccc tctgtctact 6165 tcaacccctt ctccggctct cctgggcatt atccggacga gttcatacca aacttcgacg 6225 caatcagcga gtcagtggat ggctatgatt gatgtctaat ggtggcgcgg ctgagctagc 6285 tcgactgcga catctagacc actgccgccg ctttcgctgc tttgcccgag aactcaccga 6345 gttcatctac ttcgaaatac ccgaggagca ccctcaagga ccggcccacg gagtgcgtat 6405 taccatcgaa ggggggatag actctcgcct gcatcggatc ttctgccagc gacccgtgct 6465 aatcgagcgc gaccagggaa acaccacagt ctccatctac tgcatctgta accaccccgg 6525 attgc atg aaa gcc ttt gct gtc tta ttt gtg ctg agt tta ata aaa act 6575 Met Lys Ala Phe Ala Val Leu Phe Val Leu Ser Leu Ile Lys Thr 405 410 415 gag tta aga ctc tcc tac gga cta cca att ctt caa ctc gga ctt tat 6623 Glu Leu Arg Leu Ser Tyr Gly Leu Pro Ile Leu Gln Leu Gly Leu Tyr 420 425 430 aac aat cag acc ctc cgt tca agt cag aag acc cca acc ctt cct ctg 6671 Asn Asn Gln Thr Leu Arg Ser Ser Gln Lys Thr Pro Thr Leu Pro Leu 435 440 445 atc cag gaa tct aat tct acc tcc cca gca cca cac ttt act agc ctt 6719 Ile Gln Glu Ser Asn Ser Thr Ser Pro Ala Pro His Phe Thr Ser Leu 450 455 460 ccc gaa act aac aac ctc gga gct caa ctg cac cac ttt tcc aga agc 6767 Pro Glu Thr Asn Asn Leu Gly Ala Gln Leu His His Phe Ser Arg Ser 465 470 475 480 ctt ctc tct gcc aat act acc act ccc aga acc gga ggt gag ctc cgt 6815 Leu Leu Ser Ala Asn Thr Thr Thr Pro Arg Thr Gly Gly Glu Leu Arg 485 490 495 ggt ctt cct aat aac aac ccc tgg gtg gta act ggg ttt gta acg cta 6863 Gly Leu Pro Asn Asn Asn Pro Trp Val Val Thr Gly Phe Val Thr Leu 500 505 510 ggt gta gtt gcg ggt ggg ctt gtg ctt gtc ctt tgc tac cta tac aca 6911 Gly Val Val Ala Gly Gly Leu Val Leu Val Leu Cys Tyr Leu Tyr Thr 515 520 525 cct tgc tgt gct tat tta gta atc ttg tgt tgc tgg ttt aag aaa tgg 6959 Pro Cys Cys Ala Tyr Leu Val Ile Leu Cys Cys Trp Phe Lys Lys Trp 530 535 540 ggg ccc tac tagtcgcgct tgctttactt tcacttttgg atctgggctc 7008 Gly Pro Tyr 545 tactatgcta gttcagcctg tactatttga tccatgcctc aattttgatc cagacaactg 7068 cacactcact tttgctccag aggctggccg ctgtggagtt cttattaggt gcggacggga 7128 atgcagtccc attgaaatac accacaataa caaaatttgg aacaatacct tattcaccac 7188 atggcagcca ggagaccctg agtggtatac tgtctctgtc cgtggtcctg acggttccat 7248 ccgcactgct aataacactt ttatttttgc tgagatgtgc gatctgacca tgttcatgag 7308 caaacagtat aacctatggc ctccaagcaa ggagaacatt gtggcattct ccattgctta 7368 tttcttgtgt acgtgtctca ttactgctat tctatgtatc tgcatacact tgcttatttg 7428 ccaccgccac agaaacagca atg agg aaa aag aga aaa tgc ctt gag ctt ttt 7481 Met Arg Lys Lys Arg Lys Cys Leu Glu Leu Phe 550 555 ctc att ttt gtt ttt ttt ttg ttt aca gcc atg gct tca gtt ata gct 7529 Leu Ile Phe Val Phe Phe Leu Phe Thr Ala Met Ala Ser Val Ile Ala 560 565 570 cta att att gcc agc att ctc act gcc gca cag gga caa aca att gtc 7577 Leu Ile Ile Ala Ser Ile Leu Thr Ala Ala Gln Gly Gln Thr Ile Val 575 580 585 590 tat att acc tta ggt cat aac cac act ctt ata gga ccc caa att agt 7625 Tyr Ile Thr Leu Gly His Asn His Thr Leu Ile Gly Pro Gln Ile Ser 595 600 605 tca cag gtt ata tgg acc aaa ctt gga agt gtt gat tat ttt gac ata 7673 Ser Gln Val Ile Trp Thr Lys Leu Gly Ser Val Asp Tyr Phe Asp Ile 610 615 620 atc tgc aac aga act aaa cca ata ttt gta acc tgt aac aaa caa aat 7721 Ile Cys Asn Arg Thr Lys Pro Ile Phe Val Thr Cys Asn Lys Gln Asn 625 630 635 ctc acc tta att aat gtt agc gaa att tac agc ggt tac tat tat ggt 7769 Leu Thr Leu Ile Asn Val Ser Glu Ile Tyr Ser Gly Tyr Tyr Tyr Gly 640 645 650 tat gac aga cac agc agt gaa tat aaa aat tac cta gtt cgc ata act 7817 Tyr Asp Arg His Ser Ser Glu Tyr Lys Asn Tyr Leu Val Arg Ile Thr 655 660 665 670 caa ccc aaa acc aca aaa atg cca aat aag gca aaa att caa atg gtt 7865 Gln Pro Lys Thr Thr Lys Met Pro Asn Lys Ala Lys Ile Gln Met Val 675 680 685 agc gca tta gaa cat ctt aca tat ccc acc aca ccc gat gag aga aac 7913 Ser Ala Leu Glu His Leu Thr Tyr Pro Thr Thr Pro Asp Glu Arg Asn 690 695 700 att cca aat tca atg att gcc att att gcg gcg gtg gca gtg gga atg 7961 Ile Pro Asn Ser Met Ile Ala Ile Ile Ala Ala Val Ala Val Gly Met 705 710 715 gca cta ata ata att tgt atg ttc cta tat gct tgt tac tgt aga aag 8009 Ala Leu Ile Ile Ile Cys Met Phe Leu Tyr Ala Cys Tyr Cys Arg Lys 720 725 730 ttt cat cac aaa cag gat tcc cta cta aat ttt tgacatttaa ttttttatac 8062 Phe His His Lys Gln Asp Ser Leu Leu Asn Phe 735 740 745 agctatggtt tccactacag ccttttttgt tattagtagc cttgcagctg tcacttatgg 8122 tcgctcacac ctcactgtaa ctgttggctc aacttgtaca ctacaaggac cccaagaagg 8182 gcatgtcagt tggtggagaa tatgatagtg gatggttcat taggccatgt gaccagcctg 8242 gtaacaaatt tttctgcaac gggagagact tgaccattat taacatcaca gtaaatgacc 8302 agggcttcta ttatggaact aactataaaa ataacttaga ttacaacatt atcgtagtgc 8362 cagccaccac tccagctccc cgcaaaacca ctttctttag cagcagtgcc agtatttcta 8422 aaacagcttc tgcaagcttc aaaaaattcg ctttacgtaa ttccacaacc tcttccactt 8482 ccaatatgtc taaatcagta atcggcatcg ctgctgccgc gatagtggga ttaatgatta 8542 taattttgtg cataatctac tacgcctgct gctatagaaa acatgaacaa aaaagcgatc 8602 ccttgctgaa ttttgatatt taattttttt atagcatcat gaaaaaacta agtatcctag 8662 cttttatttt gtttgaaaca tttaccaatg tgcagactac tttaagtcat gatatagaga 8722 accacactac ctcttatgtg cccacaaaca ttactaccca tcccaaacat gctatgcaac 8782 tagaaatcac catgctaatt gtagttgtaa tacttattct agctatcatt ttctatttta 8842 cactatgccg ccaaatacct aatattcata aaaattctaa aagacgtccc atctattgcc 8902 ctgtgattag tcgaccccat atgactctaa atgaaatcta agatcatcta tttctctttt 8962 acagtatggt gaacaccaat catgattcct agaaatttct tcttcaccat actcatctgt 9022 gcttttaatg tctgtgccac cttcacagca gtagccactg caaccccaga ctgtatagga 9082 ccatttgctt catatacact tttcgctttt gtcgcttgca cctgcgtgtg tagcgtagtc 9142 tgcctggtta ttaatttttt ccaacttgta gactggatct ttgtacgact tgcctacctg 9202 cgtcaccatc ccgaataccg caatcaac atg ttg cgg cac ttc tca gac tta 9254 Met Leu Arg His Phe Ser Asp Leu 750 ttt aaa acc atg cag gct ata cta cca gtc att ctg ctt ctg ttg ctc 9302 Phe Lys Thr Met Gln Ala Ile Leu Pro Val Ile Leu Leu Leu Leu Leu 755 760 765 ccc tgc gat acc tta acc ccc gtc gct aat cgt acc cca cct gaa caa 9350 Pro Cys Asp Thr Leu Thr Pro Val Ala Asn Arg Thr Pro Pro Glu Gln 770 775 780 785 ctt aga aaa tgc aaa ttc caa caa cca tgg aca ttc ctt gat tgc tac 9398 Leu Arg Lys Cys Lys Phe Gln Gln Pro Trp Thr Phe Leu Asp Cys Tyr 790 795 800 cga gaa aaa tct gat ttc cct aca tac tgg att atg atc att gga att 9446 Arg Glu Lys Ser Asp Phe Pro Thr Tyr Trp Ile Met Ile Ile Gly Ile 805 810 815 gtc aat cta gtt tct tgc aca cta ttc tct ttc ctt gtt tat cat ttt 9494 Val Asn Leu Val Ser Cys Thr Leu Phe Ser Phe Leu Val Tyr His Phe 820 825 830 ttt gat ttt gga tgg aat gcc ccc aat gca ctc act tac cca caa gaa 9542 Phe Asp Phe Gly Trp Asn Ala Pro Asn Ala Leu Thr Tyr Pro Gln Glu 835 840 845 cca gag gaa cat atc cca cta cag aac atg caa cag cca ata gct ata 9590 Pro Glu Glu His Ile Pro Leu Gln Asn Met Gln Gln Pro Ile Ala Ile 850 855 860 865 ata gat tat gac aat gag cca cag ccc tcg ctg ctt cct gct att agt 9638 Ile Asp Tyr Asp Asn Glu Pro Gln Pro Ser Leu Leu Pro Ala Ile Ser 870 875 880 tac ttc aac cta acc ggt gga gat gac tgacc 9670 Tyr Phe Asn Leu Thr Gly Gly Asp Asp 885 890 <210> 189 <211> 203 <212> PRT <213> Simian adenovirus 35 <400> 189 Met Ser Pro Pro Cys Phe Ile Phe Phe Leu Lys Tyr Ser Thr Trp Ser 1 5 10 15 Glu Cys Thr Asn His Thr Ala Ala Ser Ser Arg Pro Ser Thr Cys Ala 20 25 30 His Arg Ser Arg Leu Val Thr Pro Pro His Lys Lys Pro Ala Ser Cys 35 40 45 Lys Val Gln Pro Trp Pro Ala Gly Pro Glu Thr Ala Pro Ala Ser Lys 50 55 60 Ser Ser Glu Pro Ser Ser Glu Thr Leu Ala Val Asp Pro Thr Ser Trp 65 70 75 80 Glu Pro Leu Thr Asn Ala Ser Arg Gly Leu Trp Leu Gln Thr Ser Trp 85 90 95 Pro Ala Pro Leu Ser Thr Gln Pro Val Ala Arg Arg Gly Glu Ser Thr 100 105 110 Gly Trp Leu Leu Val Gly Thr Arg Ala Pro Thr His Ala Thr Phe Leu 115 120 125 Ile Arg Leu Asp Ser Arg Met Thr Val Ser Ser Arg Ser Thr Ser Leu 130 135 140 Asn Thr Arg Gly Tyr Cys Ala Ala Ala Pro Leu Leu Leu Arg Ile Ala 145 150 155 160 Ala Leu Pro Trp Lys Ser Pro Pro Lys Pro Cys Arg Val Arg Ala Pro 165 170 175 Pro Leu Val Asp Phe Phe Ala Ala Cys Phe Ser Met Pro Leu Tyr Thr 180 185 190 Gly Gln Thr Ala Pro Trp Thr Val Thr Pro Pro 195 200 <210> 190 <211> 198 <212> PRT <213> Simian adenovirus 35 <400> 190 Met Ser Gln Arg Arg Gly Asn Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Thr Glu Glu Met Glu 20 25 30 Asp Trp Asp Ser Gln Ala Glu Glu Glu Glu Asp Ser Leu Glu Glu Asp 35 40 45 Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu Ala Thr 50 55 60 Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Ala Thr Asp 65 70 75 80 Asn Thr Thr Ala Thr Ile Ser Val Pro Gly Arg Gly Val Gln His Arg 85 90 95 Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Leu Pro Asn Ala Thr Thr 100 105 110 Ala Ser Lys Thr Gly Lys Lys Glu Arg Gln Gly Tyr Lys Ser Trp Arg 115 120 125 Gly His Lys Asn Ala Ile Ile Ser Cys Leu His Glu Cys Gly Gly Asn 130 135 140 Ile Ser Phe Thr Arg Arg Tyr Leu Leu Phe His His Gly Val Asn Phe 145 150 155 160 Pro Arg Asn Val Leu His Tyr Tyr Arg His Leu His Ser Pro Tyr Tyr 165 170 175 Ser Gln Gln Ala Ser Ala Glu Lys Asp Asn Ser Ser Lys Asn Leu Gln 180 185 190 Gln Lys Thr Ser Ser Ser 195 <210> 191 <211> 146 <212> PRT <213> Simian adenovirus 35 <400> 191 Met Lys Ala Phe Ala Val Leu Phe Val Leu Ser Leu Ile Lys Thr Glu 1 5 10 15 Leu Arg Leu Ser Tyr Gly Leu Pro Ile Leu Gln Leu Gly Leu Tyr Asn 20 25 30 Asn Gln Thr Leu Arg Ser Ser Gln Lys Thr Pro Thr Leu Pro Leu Ile 35 40 45 Gln Glu Ser Asn Ser Thr Ser Pro Ala Pro His Phe Thr Ser Leu Pro 50 55 60 Glu Thr Asn Asn Leu Gly Ala Gln Leu His His Phe Ser Arg Ser Leu 65 70 75 80 Leu Ser Ala Asn Thr Thr Thr Pro Arg Thr Gly Gly Glu Leu Arg Gly 85 90 95 Leu Pro Asn Asn Asn Pro Trp Val Val Thr Gly Phe Val Thr Leu Gly 100 105 110 Val Val Ala Gly Gly Leu Val Leu Val Leu Cys Tyr Leu Tyr Thr Pro 115 120 125 Cys Cys Ala Tyr Leu Val Ile Leu Cys Cys Trp Phe Lys Lys Trp Gly 130 135 140 Pro Tyr 145 <210> 192 <211> 198 <212> PRT <213> Simian adenovirus 35 <400> 192 Met Arg Lys Lys Arg Lys Cys Leu Glu Leu Phe Leu Ile Phe Val Phe 1 5 10 15 Phe Leu Phe Thr Ala Met Ala Ser Val Ile Ala Leu Ile Ile Ala Ser 20 25 30 Ile Leu Thr Ala Ala Gln Gly Gln Thr Ile Val Tyr Ile Thr Leu Gly 35 40 45 His Asn His Thr Leu Ile Gly Pro Gln Ile Ser Ser Gln Val Ile Trp 50 55 60 Thr Lys Leu Gly Ser Val Asp Tyr Phe Asp Ile Ile Cys Asn Arg Thr 65 70 75 80 Lys Pro Ile Phe Val Thr Cys Asn Lys Gln Asn Leu Thr Leu Ile Asn 85 90 95 Val Ser Glu Ile Tyr Ser Gly Tyr Tyr Tyr Gly Tyr Asp Arg His Ser 100 105 110 Ser Glu Tyr Lys Asn Tyr Leu Val Arg Ile Thr Gln Pro Lys Thr Thr 115 120 125 Lys Met Pro Asn Lys Ala Lys Ile Gln Met Val Ser Ala Leu Glu His 130 135 140 Leu Thr Tyr Pro Thr Thr Pro Asp Glu Arg Asn Ile Pro Asn Ser Met 145 150 155 160 Ile Ala Ile Ile Ala Ala Val Ala Val Gly Met Ala Leu Ile Ile Ile 165 170 175 Cys Met Phe Leu Tyr Ala Cys Tyr Cys Arg Lys Phe His His Lys Gln 180 185 190 Asp Ser Leu Leu Asn Phe 195 <210> 193 <211> 145 <212> PRT <213> Simian adenovirus 35 <400> 193 Met Leu Arg His Phe Ser Asp Leu Phe Lys Thr Met Gln Ala Ile Leu 1 5 10 15 Pro Val Ile Leu Leu Leu Leu Leu Pro Cys Asp Thr Leu Thr Pro Val 20 25 30 Ala Asn Arg Thr Pro Pro Glu Gln Leu Arg Lys Cys Lys Phe Gln Gln 35 40 45 Pro Trp Thr Phe Leu Asp Cys Tyr Arg Glu Lys Ser Asp Phe Pro Thr 50 55 60 Tyr Trp Ile Met Ile Ile Gly Ile Val Asn Leu Val Ser Cys Thr Leu 65 70 75 80 Phe Ser Phe Leu Val Tyr His Phe Phe Asp Phe Gly Trp Asn Ala Pro 85 90 95 Asn Ala Leu Thr Tyr Pro Gln Glu Pro Glu Glu His Ile Pro Leu Gln 100 105 110 Asn Met Gln Gln Pro Ile Ala Ile Ile Asp Tyr Asp Asn Glu Pro Gln 115 120 125 Pro Ser Leu Leu Pro Ala Ile Ser Tyr Phe Asn Leu Thr Gly Gly Asp 130 135 140 Asp 145 <210> 194 <211> 880 <212> DNA <213> Simian adenovirus 35 <220> <221> CDS <222> (1)..(580) <223> label=Ela <220> <221> CDS <222> (672)..(877) <223> label=Ela <400> 194 atg aga cac ctg cgt ttc ctg tcc cag gag ata gtc tcc act gaa act 48 Met Arg His Leu Arg Phe Leu Ser Gln Glu Ile Val Ser Thr Glu Thr 1 5 10 15 ggg aat gaa ata ctg cag ttt gtg gta aat act ctg atg gga gac gat 96 Gly Asn Glu Ile Leu Gln Phe Val Val Asn Thr Leu Met Gly Asp Asp 20 25 30 cca gag ccg cct gag cca tct ttt gat cct cct acg ctt cat gaa tta 144 Pro Glu Pro Pro Glu Pro Ser Phe Asp Pro Pro Thr Leu His Glu Leu 35 40 45 tat gat tta gag gta gac gga ccg gag gac cct aat gag gac gac gtg 192 Tyr Asp Leu Glu Val Asp Gly Pro Glu Asp Pro Asn Glu Asp Asp Val 50 55 60 aat ggg ttt ttt act gat tct atg tta tta gct gct aat gag gga gtg 240 Asn Gly Phe Phe Thr Asp Ser Met Leu Leu Ala Ala Asn Glu Gly Val 65 70 75 80 gat tta gac cca cct tct gga act ctt gat act cca ggg gtg att gtg 288 Asp Leu Asp Pro Pro Ser Gly Thr Leu Asp Thr Pro Gly Val Ile Val 85 90 95 gaa agc gac ata aat ggg aaa aat tta cct gat ttg ggt gct gct gaa 336 Glu Ser Asp Ile Asn Gly Lys Asn Leu Pro Asp Leu Gly Ala Ala Glu 100 105 110 ttg gac ttg cac tgc tat gaa gag ggt ttt cct ccg agt gat gat gaa 384 Leu Asp Leu His Cys Tyr Glu Glu Gly Phe Pro Pro Ser Asp Asp Glu 115 120 125 gat gtg gag aat gag cag tca att cag acc gca gcg ggt gag gga gtg 432 Asp Val Glu Asn Glu Gln Ser Ile Gln Thr Ala Ala Gly Glu Gly Val 130 135 140 aaa gca gcc agt gat ggt ttt aag ttg gac tgc ccg atg ctg cct gga 480 Lys Ala Ala Ser Asp Gly Phe Lys Leu Asp Cys Pro Met Leu Pro Gly 145 150 155 160 cat ggc tgt aag tct tgt gaa ttt cac agg aaa aat act gga gta aaa 528 His Gly Cys Lys Ser Cys Glu Phe His Arg Lys Asn Thr Gly Val Lys 165 170 175 gaa ata tta tgc tcg ctt tgt tat atg aga gcg cat tgc cac ttt att 576 Glu Ile Leu Cys Ser Leu Cys Tyr Met Arg Ala His Cys His Phe Ile 180 185 190 tac a gtaagtgtgt ttaagttaaa tttaaaggaa cagtagctgt ttttataact 630 Tyr cttggatggg tgatttatgt tttgcttgtg attttttata g gt cct gtg tct gat 685 Ser Pro Val Ser Asp 195 gct gat gaa tcg cct tct cct gat tca act acc tca cct cct gaa att 733 Ala Asp Glu Ser Pro Ser Pro Asp Ser Thr Thr Ser Pro Pro Glu Ile 200 205 210 cag gca ccc gtc cct gca aat gta tgc aag ccc att cct gtg aag ctt 781 Gln Ala Pro Val Pro Ala Asn Val Cys Lys Pro Ile Pro Val Lys Leu 215 220 225 230 aag cct ggg aaa cgc cct gct gtg gat aaa ctt gag gat ttg ctg gag 829 Lys Pro Gly Lys Arg Pro Ala Val Asp Lys Leu Glu Asp Leu Leu Glu 235 240 245 ggt gtg gat gaa cct ttg gac ttg tgt acc cgg aaa ata cca agg caa 877 Gly Val Asp Glu Pro Leu Asp Leu Cys Thr Arg Lys Ile Pro Arg Gln 250 255 260 tga 880 <210> 195 <211> 262 <212> PRT <213> Simian adenovirus 35 <400> 195 Met Arg His Leu Arg Phe Leu Ser Gln Glu Ile Val Ser Thr Glu Thr 1 5 10 15 Gly Asn Glu Ile Leu Gln Phe Val Val Asn Thr Leu Met Gly Asp Asp 20 25 30 Pro Glu Pro Pro Glu Pro Ser Phe Asp Pro Pro Thr Leu His Glu Leu 35 40 45 Tyr Asp Leu Glu Val Asp Gly Pro Glu Asp Pro Asn Glu Asp Asp Val 50 55 60 Asn Gly Phe Phe Thr Asp Ser Met Leu Leu Ala Ala Asn Glu Gly Val 65 70 75 80 Asp Leu Asp Pro Pro Ser Gly Thr Leu Asp Thr Pro Gly Val Ile Val 85 90 95 Glu Ser Asp Ile Asn Gly Lys Asn Leu Pro Asp Leu Gly Ala Ala Glu 100 105 110 Leu Asp Leu His Cys Tyr Glu Glu Gly Phe Pro Pro Ser Asp Asp Glu 115 120 125 Asp Val Glu Asn Glu Gln Ser Ile Gln Thr Ala Ala Gly Glu Gly Val 130 135 140 Lys Ala Ala Ser Asp Gly Phe Lys Leu Asp Cys Pro Met Leu Pro Gly 145 150 155 160 His Gly Cys Lys Ser Cys Glu Phe His Arg Lys Asn Thr Gly Val Lys 165 170 175 Glu Ile Leu Cys Ser Leu Cys Tyr Met Arg Ala His Cys His Phe Ile 180 185 190 Tyr Ser Pro Val Ser Asp Ala Asp Glu Ser Pro Ser Pro Asp Ser Thr 195 200 205 Thr Ser Pro Pro Glu Ile Gln Ala Pro Val Pro Ala Asn Val Cys Lys 210 215 220 Pro Ile Pro Val Lys Leu Lys Pro Gly Lys Arg Pro Ala Val Asp Lys 225 230 235 240 Leu Glu Asp Leu Leu Glu Gly Val Asp Glu Pro Leu Asp Leu Cys Thr 245 250 255 Arg Lys Ile Pro Arg Gln 260 <210> 196 <211> 880 <212> DNA <213> Simian adenovirus 35 <220> <221> CDS <222> (12)..(360) <223> label=33K <220> <221> CDS <222> (530)..(879) <223> label=33K <400> 196 gttctctcag g atg tct cag cgc cga gga aac aag aag ttg aaa gtg cag 50 Met Ser Gln Arg Arg Gly Asn Lys Lys Leu Lys Val Gln 1 5 10 ctg ccg ccc cca gag gat atg gag gaa gac tgg gac agt cag aca gag 98 Leu Pro Pro Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Thr Glu 15 20 25 gag atg gaa gat tgg gac agc cag gca gag gag gag gag gac agc ctg 146 Glu Met Glu Asp Trp Asp Ser Gln Ala Glu Glu Glu Glu Asp Ser Leu 30 35 40 45 gag gaa gac agt ttg gag gag gaa gac gag gag gca gag gag gtg gaa 194 Glu Glu Asp Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu 50 55 60 gaa gca acc gcc gcc aaa cag ttg tcc tcg gcg gcg gag aca agc aag 242 Glu Ala Thr Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys 65 70 75 gcc aca gac aac acc aca gct acc atc tcc gtt ccg ggt cgg ggg gtc 290 Ala Thr Asp Asn Thr Thr Ala Thr Ile Ser Val Pro Gly Arg Gly Val 80 85 90 cag cac cgt ccc aac agt aga tgg gat gag acc ggg cga ctc ccg aat 338 Gln His Arg Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Leu Pro Asn 95 100 105 gcg acc acc gct tct aag act g gtaagaagga gcggcaggga tacaagtcct 390 Ala Thr Thr Ala Ser Lys Thr 110 115 ggcgggggca taagaacgct atcatatcct gcttgcatga atgcgggggc aacatatcct 450 tcacccgccg ctacctgctc ttccaccacg gggtgaactt cccccgcaat gtcttgcatt 510 actaccgtca cctccacag cc cct act aca gcc agc aag cct cgg cag aaa 561 Ala Pro Thr Thr Ala Ser Lys Pro Arg Gln Lys 120 125 aag aca aca gca gca aga acc tcc agc aga aaa cca gca gca gtt aga 609 Lys Thr Thr Ala Ala Arg Thr Ser Ser Arg Lys Pro Ala Ala Val Arg 130 135 140 aca ccc aca gca ggt gca aca gga gga gga ctg aga atc aca gcg aac 657 Thr Pro Thr Ala Gly Ala Thr Gly Gly Gly Leu Arg Ile Thr Ala Asn 145 150 155 gag cca gcg cag acc cga gag ctg aga aac cgg att ttt cca acc ctc 705 Glu Pro Ala Gln Thr Arg Glu Leu Arg Asn Arg Ile Phe Pro Thr Leu 160 165 170 175 tat gcc atc ttc caa cag agt cgg ggg caa gag cag gaa ctg aaa gta 753 Tyr Ala Ile Phe Gln Gln Ser Arg Gly Gln Glu Gln Glu Leu Lys Val 180 185 190 aaa aac cga tct ttg cgc tcg ctc acc cga agt tgt ttg tat cac aag 801 Lys Asn Arg Ser Leu Arg Ser Leu Thr Arg Ser Cys Leu Tyr His Lys 195 200 205 agc gaa gac caa ctt cag cgc act ctc gag gac gcc gag gct ctc ttc 849 Ser Glu Asp Gln Leu Gln Arg Thr Leu Glu Asp Ala Glu Ala Leu Phe 210 215 220 aac aag tac tgc gcg ctc act ctt aaa gag t 880 Asn Lys Tyr Cys Ala Leu Thr Leu Lys Glu 225 230 <210> 197 <211> 233 <212> PRT <213> Simian adenovirus 35 <400> 197 Met Ser Gln Arg Arg Gly Asn Lys Lys Leu Lys Val Gln Leu Pro Pro 1 5 10 15 Pro Glu Asp Met Glu Glu Asp Trp Asp Ser Gln Thr Glu Glu Met Glu 20 25 30 Asp Trp Asp Ser Gln Ala Glu Glu Glu Glu Asp Ser Leu Glu Glu Asp 35 40 45 Ser Leu Glu Glu Glu Asp Glu Glu Ala Glu Glu Val Glu Glu Ala Thr 50 55 60 Ala Ala Lys Gln Leu Ser Ser Ala Ala Glu Thr Ser Lys Ala Thr Asp 65 70 75 80 Asn Thr Thr Ala Thr Ile Ser Val Pro Gly Arg Gly Val Gln His Arg 85 90 95 Pro Asn Ser Arg Trp Asp Glu Thr Gly Arg Leu Pro Asn Ala Thr Thr 100 105 110 Ala Ser Lys Thr Ala Pro Thr Thr Ala Ser Lys Pro Arg Gln Lys Lys 115 120 125 Thr Thr Ala Ala Arg Thr Ser Ser Arg Lys Pro Ala Ala Val Arg Thr 130 135 140 Pro Thr Ala Gly Ala Thr Gly Gly Gly Leu Arg Ile Thr Ala Asn Glu 145 150 155 160 Pro Ala Gln Thr Arg Glu Leu Arg Asn Arg Ile Phe Pro Thr Leu Tyr 165 170 175 Ala Ile Phe Gln Gln Ser Arg Gly Gln Glu Gln Glu Leu Lys Val Lys 180 185 190 Asn Arg Ser Leu Arg Ser Leu Thr Arg Ser Cys Leu Tyr His Lys Ser 195 200 205 Glu Asp Gln Leu Gln Arg Thr Leu Glu Asp Ala Glu Ala Leu Phe Asn 210 215 220 Lys Tyr Cys Ala Leu Thr Leu Lys Glu 225 230 <210> 198 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 198 cgcgccgagc attcatgctt gtacgtaccc acgcacagct ttaaacattt g 51 <210> 199 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 199 aattcaaatg tttaaagctg tgcgtgggta cgtacaagca tgaatgctcg g 51 <210> 200 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 200 taccaccagc ggcgcgccag acatcaag 28 <210> 201 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 201 aaatggaatt caaatgttta aagctgtg 28 <210> 202 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 202 ttgtagcata gtttgcctgg 20

Claims (21)

  1. (a) SAdV-28의 헥손 단백질, SEQ ID NO: 11의 아미노산 1 내지 944; SAdV-27의 헥손 단백질, SEQ ID NO: 49의 아미노산 1 내지 956; SAdV-29의 헥손 단백질, SEQ ID NO: 81의 아미노산 1 내지 954; SAdV-32의 헥손 단백질, SEQ ID NO: 113의 아미노산 1 내지 955; SAdV-33의 헥손 단백질, SEQ ID NO: 144의 아미노산 1 내지 951; SAdV-35의 헥손 단백질, SEQ ID NO: 176의 아미노산 1 내지 956;
    (b) SAdV-28의 펜톤 단백질, SEQ ID NO: 6의 아미노산 1 내지 582; SAdV-27의 펜톤 단백질, SEQ ID NO: 44의 아미노산 1 내지 562; SAdV-29의 펜톤 단백질, SEQ ID NO: 76의 아미노산 1 내지 576; SAdV-32의 펜톤 단백질, SEQ ID NO: 108의 아미노산 1 내지 585; SAdV-33의 펜톤 단백질, SEQ ID NO: 139의 아미노산 1 내지 571; SAdV-35의 펜톤 단백질, SEQ ID NO: 171의 아미노산 1 내지 563; 및
    (c) SAdV-28의 섬유 단백질, SEQ ID NO: 21의 아미노산 1 내지 323, SAdV-27의 섬유 단백질, SEQ ID NO: 59의 아미노산 1 내지 325; SAdV-29의 섬유 단백질, SEQ ID NO: 91의 아미노산 1 내지 324; SAdV-32의 섬유 단백질, SEQ ID NO: 123의 아미노산 1 내지 319; SAdV-33의 섬유 단백질, SEQ ID NO: 154의 아미노산 1 내지 325; SAdV-35의 섬유 단백질, SEQ ID NO: 185의 아미노산 1 내지 353;
    로 구성되는 군으로부터 선택되는 캡시드 단백질을 포함하는 캡시드를 가지며,
    상기 캡시드는 숙주 세포에서 그것의 전사, 번역 및/또는 발현을 지시하는 발현 조절 서열에 작동가능하게 연결된 유전자를 전달하는 이종성 분자를 단백질막으로 싸는 아데노바이러스.
  2. 제 1 항에 있어서, 복제 및 단백질 막화에 필요한 5' 및 3' 아데노바이러스 시스-구성요소를 추가로 포함하는 것을 특징으로 하는 아데노바이러스.
  3. 제 1 항에 있어서, 상기 아데노바이러스는 E1 유전자의 모두 또는 일부를 결핍하는 것을 특징으로 하는 아데노바이러스.
  4. 제 3 항에 있어서, 상기 아데노바이러스는 복제-결함인 것을 특징으로 하는 아데노바이러스.
  5. 제 5 항에 있어서, 상기 바이러스는 하이브리드 캡시드인 것을 특징으로 하는 아데노바이러스.
  6. 제 5 항에 있어서, 상기 벡터는 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33, 및 SAdV-35로부터 선택되는 하나 이상의 캡시드 단백질을 포함하는 것을 특징으로 하는 아데노바이러스.
  7. SAdV 헥손 단백질의 단편은 길이에 있어서 약 50개의 아미노산의 N-말단 또는 C-말단 절단을 가지는 SEQ ID NO: 11, 49, 81, 113, 144 또는 176의 SAdV 헥손 단백질 또는
    SEQ ID NO: 11, 49, 81, 113, 144 또는 176의 아미노산 잔기 125 내지 443;
    SEQ ID NO: 11, 49, 81, 113, 144 또는 176의 아미노산 잔기 138 내지 441;
    SEQ ID NO: 11, 49, 81, 113, 144 또는 176의 아미노산 잔기 138 내지 163;
    SEQ ID NO: 11, 49, 81, 113, 144 또는 176의 아미노산 잔기 170 내지 176; 및
    SEQ ID NO: 11, 49, 81, 113, 144 또는 176의 아미노산 잔기 404 내지 430으로 구성되는 군으로부터 선택되는 유인원 아데노바이러스 헥손 단백질의 단편 및 SAdV에 이종성인 핵산 서열을 함유하는 헥손을 포함하는 캡시드를 가지는 재조합 아데노바이러스.
  8. 제 7 항에 있어서, 캡시드는 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 또는 SAdV-35 섬유 단백질을 추가로 포함하는 것을 특징으로 하는 재조합 아데노바이러스.
  9. 제 7 항에 있어서, 캡시드는 추가로 SAdV-28, SAdV-27, SAdV-29, SAdV-32, SAdV-33 또는 SAdV-35 펜톤 단백질을 포함하는 것을 특징으로 하는 재조합 아데노바이러스.
  10. 제 7 항에 있어서, 상기 아데노바이러스는 복제 및 단백질 막화에 필요한 5' 및 3' 아데노바이러스 시스-구성요소를 포함하는 슈도타입화된 아데노바이러스이고, 상기 시스-구성요소는 아데노바이러스 5' 역위 말단 반복 및 아데노바이러스 3' 역위 말단 반복을 포함하는 것을 특징으로 하는 재조합 아데노바이러스.
  11. 제 7 항에 있어서, 아데노바이러스는 숙주 세포에서 생성물의 발현을 지시하는 서열에 작동가능하게 연결된 생성물을 암호화하는 핵산 서열을 포함하는 것을 특징으로 하는 재조합 아데노바이러스.
  12. 제 7 항에 있어서, 재조합 아데노바이러스는 하나 이상의 아데노바이러스 유전자를 포함하는 것을 특징으로 하는 재조합 아데노바이러스.
  13. 제 7 항에 있어서, 재조합 아데노바이러스는 복제-결함인 것을 특징으로 하는 재조합 아데노바이러스.
  14. 제 13 항에 있어서, 재조합 아데노바이러스는 아데노바이러스 E1에서 결실된 것을 특징으로 하는 재조합 아데노바이러스.
  15. 약학적으로 허용가능한 담체 중에 제 1 항 내지 제 14 항 중 어느 한 항의 바이러스를 포함하는 조성물.
  16. 제 1 항 내지 제 14 항 중 어느 한 항에 따르는 바이러스를 피험자에게 전달하는 단계를 포함하는 아데노바이러스 수용체를 가지는 세포를 표적화하는 방법.
  17. 유인원 아데노바이러스 28 핵산 SEQ ID NO:1의 1 내지 35610 및 그것의 보체;
    유인원 아데노바이러스 29 핵산 SEQ ID NO: 71의 1 내지 35646 및 그것의 보체;
    유인원 아데노바이러스 32 핵산 SEQ ID NO: 103의 1 내지 35588 및 그것의 보체;
    유인원 아데노바이러스 33 핵산 SEQ ID NO: 134의 1 내지 35694 및 그것의 보체; 및
    유인원 아데노바이러스 35 핵산 SEQ ID NO: 166의 1 내지 35606 및 그것의 보체
    로 구성되는 군으로부터 선택되는 분리된 유인원 아데노바이러스 아데노바이러스 핵산.
  18. (a) 5' 역위 말단 반복 (ITR) 서열;
    (b) 아데노바이러스 E1a 영역;
    (c) 아데노바이러스 E1b 영역, 또는 작은 T, 거대한 T, 및 IX 영역에 대한 오픈리딩프레임으로 구성되는 군 중에서 선택되는 그것의 단편;
    (d) E2b 영역, 또는 pTP, 폴리머라아제, 및 IVa2에 대한 오픈리딩프레임으로 구성되는 군으로부터 선택되는 그것의 단편;
    (e) L1 영역, 또는 28.1 kD 단백질, 52/55 kD 단백질, 및 IIIa 단백질에 대한 오픈리딩프레임으로 구성되는 군 중에서 선택되는 그것의 단편;
    (f) L2 영역, 또는 펜톤, VII, VI, 및 pX/Mu 단백질에 대한 오픈리딩프레임으로 구성되는 군으로부터 선택되는 그것의 단편;
    (g) L3 영역, 또는 VI, 헥손, 또는 엔도프로테아제에 대한 오픈리딩프레임으로 구성되는 군으로부터 선택되는 그것의 단편;
    (h) E2a 단백질 또는 DNA-결합 단백질(DBP)에 대한 오픈리딩프레임;
    (i) L4 영역, 또는 100 kD 단백질, 33 kD 상동체, 및 VIII에 대한 오픈리딩프레임으로 구성되는 군으로부터 선택되는 그것의 단편;
    (j) E3 영역, 또는 12.5K 단백질, CR1-알파, gp19K; CR1-베타; CR1-감마; RID-알파; RID-베타; 및 14.7 K에 대한 오픈리딩프레임으로 구성되는 군으로부터 선택되는 그것의 단편;
    (k) L5 영역, 또는 섬유 단백질에 대한 오픈리딩프레임으로부터 선택되는 단편;
    (l) E4 영역, 또는 E4 ORF6/7, E4 ORF6, E4 ORF4, E4 ORF3, E4 ORF2, 및 E4 ORF1에 대한 오픈리딩프레임으로 구성되는 군으로부터 선택되는 그것의 단편; 및
    (m) SAdV-28, SEQ ID NO:1, SAdV-27, SEQ ID NO: 39, SAdV-29, SEQ ID NO: 71, SAdV-32, SEQ ID NO: 103, SAdV-33, SEQ ID NO: 134 또는 SAdV-35, SEQ ID NO: 166의 3' ITR
    로 구성되는 하나 이상의 군으로부터 선택되는 유인원 아데노바이러스 핵산 서열을 포함하는 벡터.
  19. 제 18 항에 따르는 핵산 서열에 의해 암호화되는 유인원 아데노바이러스 단백질.
  20. 하기의 군으로부터 선택되는 하나 이상의 유인원 아데노바이러스 단백질을 포함하는 조성물:
    Figure pat00009

    Figure pat00010
  21. 제 20 항에 따르는 조성물을 피험자에 전달하는 단계를 포함하며, 상기 조성물은 헥손, 펜톤 및 섬유소로부터 선택되는 하나 이상의 유인원 아데노바이러스 SAdV-28, 27, -29, -32, -32, 및 -35 단백질인 아데노바이러스 수용체를 가지는 세포를 표적화하는 방법.
KR1020167026644A 2007-11-28 2008-11-24 유인원 아과 ｂ 아데노바이러스 ｓａｄｖ-28,27,-29,-32,-33, 및 -35 및 그것의 사용 KR101761683B1 (ko)

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US454207P 2007-11-28 2007-11-28
US453407P 2007-11-28 2007-11-28
US456707P 2007-11-28 2007-11-28
US453307P 2007-11-28 2007-11-28
US453107P 2007-11-28 2007-11-28
US446607P 2007-11-28 2007-11-28
US61/004,533 2007-11-28
US61/004,542 2007-11-28
US61/004,567 2007-11-28
US61/004,531 2007-11-28
US61/004,534 2007-11-28
US61/004,466 2007-11-28
PCT/US2008/013065 WO2009073103A2 (en) 2007-11-28 2008-11-24 Simian subfamily b adenoviruses sadv-28,27,-29,-32,-33, and -35 and uses thereof

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