KR20160055118A - Cosmetic composition comprising of the mixed herb fermentes and preparation method of the same - Google Patents

Abstract

The present invention relates to a cosmetic composition which contains a fermented extract composed of soybeans, mistletoe, and imperata as an active ingredient and exhibits excellent moisturizing and relieving effects. More specifically, provided is a cosmetic composition (product name: Natural HG or NHG) exhibiting excellent moisturizing and relieving effects, by containing an extract which is produced by fermenting an extract of soybeans, mistletoe, and imperata using fermenting microorganism such as Saccharomyces cerevisiae or Lactobacillus spp., and then extracting the same thereafter.

Description

[0001] The present invention relates to a mixed fermentation extract of soybean, mistletoe and hair root, and a cosmetic composition for moisturizing and soothing the same,

The invention soybean (Soybean), mistletoe (Viscum Album), roots (Imperata Cylindrica) to The present invention relates to a cosmetic composition having excellent moisturizing and soothing effect, and more particularly, to a cosmetic composition having a fermented microorganism such as Saccharomyces cerevisiae and Lactobacillus spp . (Natural HG or NHG), which has excellent moisturizing and soothing effects, containing fermented extract obtained by first solid fermentation and second liquid fermentation using the above-mentioned fermented extract.

Patent Document: Korean Application No. 10-2001-0022014

Therefore, the inventors of the present invention have made efforts to obtain a method capable of obtaining an effective ingredient at the highest possible yield in comparison with the above-mentioned conventional techniques and apply it to cosmetics. As a result, it has been found that the method of producing the fermented soybean, mistletoe, The present invention provides a cosmetic composition which exhibits a moisturizing effect, a skin soothing effect, an anti-allergic effect and an anti-inflammatory effect of the present invention by applying it to a cosmetic composition in an enriched manner.

The objects and advantages of the present invention will become more apparent from the following detailed description of the invention when taken in conjunction with the accompanying drawings.

In order to achieve the above object, the present invention provides a cosmetic composition comprising a fermented extract obtained by fermenting soybean, mistletoe, and hair roots in a first solid fermentation and a second liquid fermentation using yeast or lactic acid bacteria, respectively, followed by extraction and filtration .

Preferably, the fermentation is carried out in the presence of a strain selected from the group consisting of soybean, mistletoe, hair fungus, yeast strain, lactic acid bacterium, Bacillus subtilis strain, or a mixture thereof, at a temperature of 20 to 40 DEG C for 1 to 7 days Which is obtained by fermentation.

Further, preferably, the cosmetic composition is characterized in that the yeast is Saccharomyces cerevisisae.

Preferably, the extraction process is performed at a temperature of 40 to 90 DEG C for 1 to 24 hours.

Preferably, the extraction is carried out using a solvent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane, And then extracting the mixture by using the cosmetic composition.

Preferably, the soybean, mistletoe, and hair fungus fermented extract are contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the cosmetic composition is characterized in that it exhibits a moisturizing effect, a skin soothing effect, an anti-allergic effect and an anti-inflammatory effect.

The cosmetic composition according to the present invention contains an effective ingredient of soybean, mistletoe, and hair fungus fermented extract and is free of skin irritation and has good solubility, affinity and stability for various bases, additives and organic solvents for cosmetics, And skin soothing effect.

The present invention provides a cosmetic composition comprising a fermented extract consisting of soybean, mistletoe, and hair root obtained by fermenting soybean, mistletoe, and hair root respectively and extracting and filtering.

According to the present invention, the fermentation extract composed of soybean, mistletoe and hair root has excellent anti-inflammatory effect.

Therefore, the cosmetic composition of the present invention comprising the fermented extract comprising soybean, mistletoe, and hair root having various functions as an active ingredient can be usefully used because of its excellent antiallergic effect.

FIG. 1 is a graph showing the erythema reducing effect of a cream containing a mixed fermentation extract composed of soybean, mistletoe and hair roots according to the present invention,
FIG. 2 is a graph showing the moisturizing effect of the mixed fermented extract composed of soybean, mistletoe,
FIG. 3 is a graph showing skin moisture retention rate of mixed fermented extract composed of soybean, mistletoe, and hair roots according to the present invention over time,
FIG. 4 is a graph showing skin sedation and anti-inflammatory effects according to the concentration of mixed fermentation extract composed of soybean, mistletoe,
FIG. 5 is a graph showing another anti-inflammatory effect according to the concentration of mixed fermentation extract composed of soybean, mistletoe,
FIG. 6 is a graph showing the anti-allergy effect depending on the concentration of the mixed fermentation extract composed of soybean, mistletoe and hair roots according to the present invention

The present invention relates to a cosmetic composition having an excellent moisturizing and soothing effect and containing a fermented extract consisting of soybean, mistletoe and hair root obtained by mixing and fermenting soybean, mistletoe, and hair root respectively, and more particularly to a cosmetic composition excellent in soybean, mistletoe, Was fermented in two stages by using yeast and lactic acid bacteria, and extracted and filtered to develop a cosmetic composition having excellent moisturizing and soothing effect containing an active ingredient of a fermented extract consisting of soybean, mistletoe and hair roots with increased physiologically active substance content Respectively.

The cosmetic composition of the present invention as described above is a cosmetic composition having excellent moisturizing and soothing effect and containing a mixed fermentation extract consisting of soybean, mistletoe and hair root obtained by mixing and fermenting soybean, mistletoe and hair root followed by extraction and filtration.

The fermented extract of the present invention composed of soybean, mistletoe, and hair roots of the present invention has excellent moisturizing effect and is suitable for use as a raw material for cosmetic compositions.

The present inventors have found that, in order to find a method capable of obtaining a physiologically active substance having a variety of effects, in particular, through a filtration process at the highest possible yield, fermentation of soybean, mistletoe, It was confirmed that the content of the active ingredient was increased through the process and it was confirmed that the moisturizing effect was obtained by applying it to the cosmetic material, thereby completing the present invention.

The soybean, mistletoe and hair roots contained in the cosmetic composition of the present invention are characterized by being composed of 1 to 50 parts by weight of soybean, 1 to 50 parts by weight of mistletoe and 1 to 50 parts by weight of hair roots on the basis of dry weight, Is obtained by performing solid fermentation and liquid fermentation for 1 to 7 days while maintaining the pH at 5 to 7 under a temperature condition of 20 to 40 캜 in a bacterium selected from the group consisting of yeast, lactic acid bacteria, Bacillus subtilis, or a mixture thereof More preferably Saccharomyces cerevisisae, and most preferably fermentation is carried out using Saccharomyces cerevisiae KCTC 7904 strain.

The soybean, mistletoe, and hair roots used in the present invention can be prepared by drying conventionally known soybean, mistletoe, and hair roots, and they are thoroughly washed, dried and pulverized, and then made into fine powder And the primary lactic acid bacteria are inoculated at a temperature of 40 to 60 DEG C for 12 to 24 hours and solid-cultured to obtain a primary culture. Add 100 g / L of soybeans, mistletoe, and hair roots to the culture medium of Saccharomyces cerevisiae (Saccharomyces cerevisiae KCTC 7904). 1 to 4% of glucose is used as a carbon source, preferably 1 to 2%, 0.1 to 1% of peptone is added, preferably 0.2 to 0.5% is further added, and yeast is inoculated again, Liquid culture. The culturing was carried out using a 5 L fermenter at a temperature of 20 to 40 ° C for 1 to 7 days while maintaining the pH at 5 to 7. After the culture, the culture is centrifuged to remove the culture bacteria first.

Preferably, the extraction is carried out at a temperature of 40 to 90 DEG C for 1 to 24 hours, preferably at a temperature of 40 to 60 DEG C for 12 to 24 hours, most preferably at a temperature of 50 DEG C Treat for 24 hours.

Also preferably, the extraction is carried out in an extraction solvent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane, , More preferably extracted with ethanol, 70% ethanol aqueous solution or water, most preferably with 70% ethanol aqueous solution.

Ethanol was added to the resulting fermented extract to make a final 70% (v / v) ethanol aqueous solution, and the mixture was refluxed three times for 5 hours. After cooling, Filter through filter paper. When the filtration is completed, the extract obtained in the extracting step is transferred to the concentration tank and concentrated under reduced pressure, followed by lyophilization. Concentration under reduced pressure was performed using a rotary vacuum concentrator. Concentration conditions were as follows: 700 ml of the extract was put into a 1,000 ml round flask, and the mixture was concentrated under reduced pressure for 2 hours while rotating at a temperature of from 650 to 1200 rpm at a temperature of from 650 to 750 mmHg at a temperature of from 650 to 750 mmHg. The concentrate was concentrated under reduced pressure, cooled to -40 ° C, maintained at -40 ° C for 2 to 4 hours, then dropped to -80 through trap freezing, kept at 10-30 mTorr for 120 minutes Lt; / RTI > Thereafter, the temperature is maintained at -20 ° C for 240 minutes, at 0 ° C for 40 minutes, at 15 ° C for 340 minutes, at 30 ° C for 130 minutes, and at 1300 minutes until completely dried.

For reference, the fermentation extract composed of soybean, mistletoe and hair roots is an extract obtained by ultra-high pressure treatment of a fermentation product obtained by fermenting soybean, mistletoe and root, or the extract is a powder obtained by vacuum-concentrating and lyophilizing the extract, Are preferred.

The fermentation extract composed of soybean, mistletoe, and hair root is not limited to a specific range, but is preferably contained in an amount of 0.0001 to 30% by weight based on the total weight of the cosmetic composition. When the content of the extract is less than 0.0001% by weight, the moisturizing effect is not exhibited. When the content of the extract is more than 30% by weight, the effect of increasing the content of the extract is insignificant, and there is a problem in safety and stability.

The ingredients contained in the cosmetic composition of the present invention include, in addition to the above-mentioned effective ingredients, the ingredients conventionally used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, And a carrier.

The cosmetic composition of the present invention may be prepared in any form conventionally produced in the art and may be in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, Oils, powder foundations, emulsion foundations, wax foundations and sprays, but are not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

The cosmetic composition of the present invention can be applied to cosmetics that can help maintain moisturization and elasticity of the skin, which increases skin's defense power in winter.

In addition, the cosmetic composition of the present invention can be applied to cosmetics which are expected to have a soothing effect of skin and an anti-inflammatory effect.

The cosmetic process of the present invention refers to all cosmetic processes using the cosmetic composition of the present invention. That is, all methods known in the art using a cosmetic composition belong to the cosmetic method of the present invention.

In addition, the present invention provides a cosmetic method for obtaining a skin moisturizing effect using a cosmetic composition comprising the soybean, mistletoe, and hair fungus fermented extract.

Refers to all cosmetic methods for applying the cosmetic composition of the present invention to human skin. That is, all methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. The cosmetic composition having excellent skin moisturizing effect, skin soothing effect, anti-allergy effect and anti-inflammatory effect according to the present invention can be used according to the usual usage method and can be used differently depending on the skin condition or taste of the user.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention, and it is to be understood by those skilled in the art that the present invention is not limited thereto It will be obvious.

Example 1 Preparation of fermented extract consisting of soybean, mistletoe and hair roots (trade name: Natural HG or NHG)

* A culture solution of Saccharomyces cerevisiae (Saccharomyces cerevisiae KCTC 7904) was added to the primary fermentation product at a level of 0.5% based on the solid content. 1 to 4% of glucose, preferably 2% (W / V) was added as a carbon source, and 0.1% of peptone was added thereto. The culture was carried out for 5 days using a 5 L fermenter while maintaining the pH at 5-7 at 37 ° C. After culturing the microorganism, the culture was centrifuged and the culture was firstly removed. The soybean, the mistletoe, and the hair roots of the cultures were removed from the primary cultures. The cultures were heated to 50 ° C, subjected to ultrahigh pressure treatment for 24 hours, and then cooled to room temperature. Ethanol was added to the final fermented product composed of soybeans treated with ultrahigh pressure, mistletoe, and hair roots to make a final 70% (v / v) ethanol aqueous solution. After refluxing for 3 hours for 5 hours, Filtered through filter paper. When the filtration is completed, the extract obtained in the extracting step is transferred to a concentration tank and concentrated under reduced pressure at 50 DEG C or below, followed by lyophilization. The lyophilized product was dissolved in 2% of 50% glycerin to prepare 20 g of a fermented extract (Example 1) composed of soybean, mistletoe and hair root.

Example 2 Preparation of Nutritive Cream Containing Mixed Fermented Extract Composed of Soybean, Mistletoe and Hair Root According to the Present Invention

A step of heating the purified water portion to 80 DEG C to dissolve, a step of emulsifying by adding an oily portion heated and dissolved at 80 DEG C, a step of adding and mixing a thickener dissolved by heating at 60 DEG C, and a step of adding and mixing cosmetic ingredients And cooled to 30 ° C., degassed and filtered, and aged at 50 ° C. to produce nutritional cream as shown in Table 1.

Classification Raw material name Weight (% by weight) Purified water portion Purified water 60.36 Propylene glycol 5.0 glycerin 6.0 Xanthan gum 0.1 Triethanolamine 0.7 Methylparaben 0.2 Phenoxyethanol 0.1 The fermented extract of the present invention 2.0 Oil component Squalane 8.0 Cetyl alcohol 2.2 Cyclopentasiloxane 2.0 Stearic acid 1.5 Glyceryl monostearate 1.0 Microcrystalline wax 0.6 PIG-100 0.3 Tocopheryl acetate 1.0 Shi Butter 1.0 Propylparaben 0.1 Orizanol 0.1 Non-sabo roll 0.2 Dimethicone 0.5 Polyoxyethylene sorbitan monostearate 0.8 Thickener Carbomer 0.24 Purified water 2.4 Beauty ingredient Sodium ascorbate 0.1 Phospholipids 1.0 Prascenta extract 0.5 Aloe extract 0.5 Tocopherol acetate 0.5 Aloe extract 1.0 Sum 100.00

Experimental Example 1 Skin irritation and protective effect of nutritional cream containing mixed fermented extract consisting of soybean, mistletoe and hair roots according to the present invention

A predetermined amount (10 m / 5 cm ² ) of the nutritional cream prepared in Example 3 was applied to the forehead of 40 healthy adults (20 men and 20 women), and the other portions were covered with aluminum foil for 30 minutes Thereafter, a long wavelength ultraviolet ray was irradiated with a dose of 10.2 J / cm < 2 > using an ultraviolet light irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, it was confirmed that the nutritional cream had no phototoxicity and had a skin protection effect because no adverse effects such as erythema were generated in the skin. Forty healthy men and women (20 males and 20 females) were divided into two groups, and the experimental group in which the nutritional cream prepared in Example 2 was applied by a predetermined amount (10 m / 5 cm ² ) , And Example 2 was prepared without containing the fermented extract composed of hair roots. As a control group, after 1 hour, 3 hours, and 6 hours, the skin elasticity was made into a cylindrical hollow having a diameter of 10 mm and an empty hollow cylindrical shape. The results showed that the skin elasticity was improved by using the nutritional cream containing the fermented extract consisting of soybean, mistletoe and hair roots of the present invention, as compared with the control group, .

<Experimental Example 2> Test for confirming the soothing effect of the nutritional cream containing the mixed fermented extract consisting of soybean, mistletoe and hair roots according to the present invention

The human skin test of the nutritional cream prepared in Example 2 was performed on the forehead portion of 40 healthy adult men (20 men and 20 women). The entire portion of the test subject was thoroughly wiped with 70% ethanol, and then the pin chamber containing 20 占 퐇 of the nutritional cream prepared in Example 2 was closed on the common portion for 24 hours by using an adhesive tape. After 24 hours, the adhesive tape and the pin chamber were removed, and the sample remaining on the skin with the gauze was gently wiped off and the skin condition was observed and again after 24 hours. As a result of the experiment, as shown in FIG. 1, in the human skin test for confirming the skin irritation of the nutritional cream containing the fermented extract composed of soybean, mistletoe and hair roots according to the present invention, no positive reaction was observed, Respectively.

Experimental Example 3 Measurement of moisturizing effect of mixed fermented extract consisting of soybean, mistletoe and hair roots according to the present invention with time

Samples of comparative experimental groups containing 0.2% of hyaluronic acid and a control group of soybean, mistletoe and herbal fermented extract were used in the same manner as in Experimental Example 3. In general, the degree of hydration of the stratum corneum, that is, the evaluation method referred to as the skin moisture content, is mainly evaluated using the electrical characteristics of the stratum corneum. The most widely used method of evaluating the water content is a capacitance method. Healthy subjects are selected and the cosmetics are applied to the entire abdomen to evaluate the electric capacity over time. In this experiment, the skin capacitance was measured using a moisture checker MY-808S (Scalar, Tokyo). The results were expressed as moisture content (%). 2, the moisture content (%) of the fermented extract composed of soybean, mistletoe and hair roots according to the present invention was found to be relatively higher than that of the control (sample 1) and the comparative experiment group (sample 2) using 0.2% of hyaluronic acid Respectively. In the case of the fermented extract consisting of soybean, mistletoe and hair roots of the present invention, the moisture content difference was about twice as much as that of the control group after 1 hour immediately after application, and the water retention amount was maintained constant up to 3 hours And that the cosmetic composition containing the fermented extract composed of soybean, mistletoe and hair roots of the present invention had excellent moisturizing effect.

In FIG. 3, the skin moisture retention ratio after 2, 4, 6, and 8 hours after application of Natural HG on the whole arm of the fermented extract consisting of soybean, mistletoe and hair roots according to the present invention was found to be significantly different I could.

<Experimental Example 4> Measurement of skin sedative and anti-inflammatory effect according to the concentration of the fermented extract of soybean, mistletoe, and hair roots according to the present invention

Samples of the control and experimental groups of soybean, mistletoe, and follicular fermentation extracts were used in the same manner as in Experimental Example 3. Various inflammatory mediators are involved in the pathogenesis of inflammation, and the causes of the disease vary. Treatment of macrophages with lipopolysaccharide (LPS), known as endotoxin, increases NO biosynthesis by inducible NOS (iNOS) expression and induces an inflammatory response. NO is a pathological secondary signal transducer secreted by many cells and regulates the activity of COX and acts synergistically to the inflammatory response. By measuring NO produced by LPS induced by LPS in Raw264.7 cells, the inflammatory response inhibitory effect of the fermented extract composed of soybean, mistletoe and hair roots according to the present invention can be determined. In FIG. 4, it was confirmed that the skin soothing effect was increased by about 23% in comparison with the control group, according to the concentration of the fermented extract composed of soybean, mistletoe and hair roots according to the present invention.

In addition, 5-LO, 5-lipoxygenase can be produced by adding molecular oxygen to an unsaturated fatty acid having 1,4- cis, cis- pentadiene (1,4-pentadiene) structure in the molecule such as linoleic acid, linolenic acid and arachidonic acid , Is an enzyme that produces 1,3- cis, trans- diene 5-hydroperoxide. It is involved in the first reaction of leucotriene biosynthesis involved in various inflammatory and allergic diseases from arachidonic acid. Thus, the inhibitory activity of lipoxygenase was measured using SLO (soybean lipoxygenase) similar to 5-LO in structural and mechanism, and as shown in FIG. 5, the fermented extract composed of soybean, mistletoe, Excellent.

<Experimental Example 5> Measurement of anti-allergic effect according to the concentration of the fermented extract of mixed soybean, mistletoe, and hair root according to the present invention

Samples of the control and experimental groups of soybean, mistletoe, and follicular fermentation extracts were used in the same manner as in Experimental Example 3. Chemical agents that cause allergies Histamine and other substances increase the vascular permeability, expand the blood vessels, smooth muscle contraction, and the action of the gland to enhance the action of the characteristic. Hexamycin is an important mediator of allergic reactions with Histamine. Hexosaminidase is released from cells and is used as an indicator of immune cell degranulation. In FIG. 6, it was confirmed that the anti-allergic effect of the fermented extract consisting of soybean, mistletoe and hair roots according to the present invention increased the skin soothing effect by about 25% as compared with the control.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, &Lt; RTI ID = 0.0 &gt; and / or &lt; / RTI &gt;

Claims (3)

A method for producing a fermented extract of soybean, mistletoe, and hair root, comprising: Each of the above-
Obtaining a mixture in which 1 to 50 parts by weight of a dry fine powder is contained in an amount of 1 to 50 parts by weight; Obtaining a primary solid culture by inoculating primary lactic acid bacteria in the mixture obtained in the above step and solid-culturing at 40 to 60 ° C for 12 to 24 hours; The secondary solid phase culture obtained by inoculating Saccharomyces cerevisiae yeast strain to the primary solid fermentation product obtained in the above step and further adding a carbon source and a nitrogen source was maintained at a pH of 5 to 7 at a temperature of 20 to 40 ° C Liquid culture for 1 to 7 days; A step of centrifuging the secondary culture obtained in the above step, refluxing with ethanol, and then filtering the mixture; and a method of producing a mixed fermented extract of soybean, mistletoe and root
A mixed fermented extract of soybean, mistletoe and hair root produced according to the method of claim 1
The skin soothing, anti-inflammatory and anti-allergic functional cosmetic composition containing the mixed fermentation extract of soybean, mistletoe and hair root of claim 2

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