KR20150066187A - Method of Manufacturing Natural Cosmetic Composition Containing the extract of Moringa oleifera - Google Patents

Abstract

The present invention relates to a nano-emulsion natural cosmetic composition including an extract of Moringa oleifera and to a method for producing the same. More specifically, the composition contains: the extract of Moringa oleifera extracted by a pure compression technology; elastin which is a constituent element of dermis; collagen; and hyaluronic acid. Therefore, the composition: has enhanced effects of preventing skin wrinkles and skin aging; does not irritate skin as the extract of Moringa oleifera extracted from Moringa tree, which is a natural material, is used; and does not pollute environment. The cosmetic composition of the present invention has excellent effects of cleansing skin, soothing skin, and reducing skin wrinkles without irritating skin.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a method for manufacturing a nano-emulsion natural cosmetic composition containing a moringa extract,

To a nanoemulsion natural cosmetic composition comprising a moringa extract.

As the age increases, skin aging occurs, such as wrinkles, loss of elasticity, and skin aging associated with age.

Aging may occur even if the metabolism of fibroblasts is not smooth due to external harmful environment. Fibroblasts are present in the dermal layer of the skin and produce collagen in extracellular epilepsy. Collagen is an important protein that accounts for 30% of the total weight of bioproteins and has a solid triple helix structure. The main functions are known to be mechanical durability of the skin, resistance of connective tissues and support of cell adhesion. Such collagen is reduced in the function of fibroblasts due to aging and photoaging induced by ultraviolet irradiation, and the amount of collagen is reduced, and collagenase activity, which degrades collagen, promotes collagen reduction. It is generally known that at 80 years of age, the thickness of the skin is reduced by about 65% compared with that of 20 years old, and this phenomenon is known to be closely related to the formation of the wrinkles of the skin.

In the dermis of the skin there is elastin present in the connective tissue with collagen. Elastin has the same elasticity as rubber elasticity and is involved in the flexibility and stretchability of the tissue. Such elastin is known to play an important role in skin elasticity and prevention of wrinkles.

<Correlation between collagen and elastin>

On the other hand, hyaluronic acid is a complex polysaccharide composed of amino acid and uronic acid, and is a high molecular compound composed of N-acetylglucosamine and glucuronic acid. It is mainly found in connective tissues such as animal joint fluid, ocular vitreous body fluid, umbilical cord, dermal layer, etc. It is highly viscous and important for preventing intrusion of bacteria or penetration of poison. In addition, if the connective tissue is damaged, the synthesis of hyaluronic acid may temporarily become active for rapid recovery.

The inventors of the present invention have studied various skin physiological activities against natural plant-derived substances that are effective on the skin without polluting the environment. As a result, Moringa extract extracted from Moringa woods in natural plant extracts has been applied to skin such as skin calming, moisturizing, It is confirmed that the composition is suitable for cosmetic use, and at the same time, when a composition containing elastin, collagen and hyaluronic acid components of the skin, which is shrinking with age, is produced, To complete the present invention.

It is therefore an object of the present invention to provide a natural cosmetic composition comprising a moringa extract.

To achieve the above object, the present invention includes a moringa extract in a cosmetic composition. In addition, it is intended to provide cosmetics that are functionally more effective by further including elastin, collagen, and hyaluronic acid.

Preferably, the present invention provides a pharmaceutical composition comprising 22 to 50 parts by weight of a moringa extract, 10 to 15 parts by weight of elastin, 5 to 10 parts by weight of collagen, 10 to 15 parts by weight of hyaluronic acid, 22 to 60 parts by weight of an organic solvent, 5 to 10 parts by weight of tocopherol, 6 to 10 parts by weight of an alkanolamide, 8 to 10 parts by weight of trollamine and 2 to 7 parts by weight of an additive.

Wherein the organic solvent is at least one selected from the group consisting of C ₁ to C ₄ alcohols, acetone, a water / alcohol mixture and 15 to 26 parts by weight of isopropyl alcohol, 2 to 8 parts by weight of polyethoxy alcohol, 5 to 26 parts by weight of an alcohol, wherein the additive comprises 0.5 to 2 parts by weight of sodium tripolyphosphate, 1 to 3 parts by weight of sodium hydroxide and 0.5 to 2 parts by weight of sodium borate.

The present invention also provides a method for producing a cosmetic composition comprising a moringa extract.

Preferably, the method for preparing a cosmetic composition comprising the moringa extract comprises: a first step of adding a Moringa extract to a container provided with a homomixer and heating the mixture to about 80 to 90 캜; A second step of adding a mixture of alkanolamide, tocopherol, elastin, collagen, hyaluronic acid and an emulsifier to the Moringa extract of the first step and stirring at a high speed for about 15 minutes to prepare a homogeneous emulsion phase mixture; A third step of cooling the emulsion phase mixture to a room temperature state; Adding an organic solvent to the mixture in the third step and stirring the mixture at a high speed for about 10 minutes to prepare a cream or mucilage-like nanocolloid; And a fifth step of preparing a water-soluble nano-emulsion by adding trolamine and an additive to the cream or mucilage-phase nanocolloid.

The nano-emulsion composition of the present invention can prevent skin irritation because no preservative is used, and exhibit an excellent effect in soothing, cleaning, moisturizing, wrinkle and skin aging prevention.

Moringa oleifera ( Moringa oleifera ) is one of the most common species of cruciferous perennials in tropical and subtropical climates. Moringa is a 4 to 8 meter high shrub spreading like an umbrella. Leaves 30 to 70 cm long are deciduous, flowers are white and fragrant, and the fruit is long pods of long and triangular shape, To 40 cm. The seeds are curved triangles, with a length of 1.2 cm, a width of 1 cm, and a length of 2 cm, with three membrane wings extending from the seeds. Moringa trees survive for months without water due to the deep roots of very rapid growth that survive extreme conditions.

The meringue is rich in oleic acid, which squeezes the fruit (seed) or uses the oil obtained by hexane extraction for edible purposes. Fruit is green when it is edible like soybean and matured seed contains about 40% oil. The oils obtained from Moringa have been widely used in the manufacture of cosmetics such as perfumes, soaps and cosmetics in addition to good quality cooking.

In traditional medicine, this Moringa oil is used to relieve pain, such as gout, rheumatism, and the like (The Indian Materia Medica, pp 811-816), and Moringa is also used as an antipyretic for oral administration (Hukkeri et al , Indian J. Pharm. Sci. (2006) 68, pp. 124-126).

This Moring paper is known to contain 539 diverse and beneficial biochemically active substances, including 90 nutrients, 46 antioxidants, and 36 anti-inflammatory agents, according to the literature. Particularly, the seeds are selected from the group consisting of sterols (campesterol, stigmasterol,? -Citosterol,? 5-avenasterol and clerosterol), fatty acids (C18: 1-oleic acid - 68 to 76%, C16: (26 to 32%), fiber, omega 6, 9, 10, tocopherol (?,? , delta: 134, 93, and 48 mg / kg, respectively), and the seeds also contain glucosinolates including 4- (α-L-rampopyranosiloxy) -benzyl glucosinolate . The leaves and seeds of Moringa species are also described as containing cytokines such as zeatin, dihydrozine, and isopentyl adenine (US 2006/0222682).

On the other hand, oils obtained from seeds by compression or a significant nonpolar extraction method (especially hexane extraction) contain in particular large amounts of triglycerides and benzylisothiocyanate components.

As used herein, the term "moringa" means a moringa outpost or a variety of organs (e.g., leaves, flowers, stems, roots and seeds) Seed), and most preferably the outpost.

The Moringa extract of the present invention is extracted with pure squeezing or a significant nonpolar extraction method (especially, hexane extraction). The moringa extract of the present invention may also be prepared by conventional methods known in the art, that is, under the conditions of ordinary temperature and pressure, using conventional solvents (e.g., water, anhydrous or hydrous lower Hot water extraction, room temperature extraction, warming extraction, ultrasonic extraction, supercritical extraction, etc.), which are conventionally used, for example, by adding water, alcohol, acetone, ethyl acetate, butyl acetate or 1,3-butylene glycol.

In addition, the extract may be further subjected to filtration or purification.

In addition, the Moringa extract of the present invention can be prepared in a powder state by an additional process such as vacuum distillation and freeze-drying or spray drying.

According to a preferred embodiment of the present invention, the moringa extract of the present invention includes not only the extract obtained by the above-mentioned extraction method, but also an extract obtained by supercritical extraction or ultrasonic extraction by decompression by carbon dioxide and high temperature.

The supercritical fluid extraction refers to supercritical fluid extraction. Generally, the supercritical fluid is extracted from the liquid and the liquid when the gas reaches the critical point at high temperature and high pressure. (J. Chromatogr. A. 1998; 479: 200-205). In addition, it has been reported that the supercritical fluid has a polarity similar to that of a nonpolar solvent.

Carbon dioxide is a supercritical fluid with both liquid and gaseous nature, with the operation of supercritical fluid equipment reaching its critical pressure and temperature, resulting in increased solubility in fat-soluble solutes. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the lipophilic substance contained in the sample is extracted into supercritical carbon dioxide.

When the supernatant carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction vessel after extracting the lipid-soluble substance, components that were not extracted by pure supercritical carbon dioxide can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract an active ingredient by using a mixed fluid in which supercritical carbon dioxide or carbon dioxide is further mixed with a solvent. These solvents may be used alone or in admixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether.

Most of the extracted samples contain carbon dioxide. Since the carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the solvent can be removed by a reduced pressure evaporator.

As the extraction solvent which can be used for the ultrasonic extraction method, one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether can be used.

The extracted sample is recovered by vacuum filtration, and the filtrate is recovered, and the extract is removed by a vacuum evaporator and freeze-dried to obtain an extract.

In addition, the Moringa extract of the present invention also includes extracts which have undergone conventional purification and / or fermentation processes. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, Fraction is also considered to be included in the moringa extract of the present invention. The fermentation product obtained by fermentation for 1 to 7 days while maintaining the pH of 5 to 7 under a temperature condition of 20 to 40 DEG C as a bacterium selected from the group consisting of yeast, lactic acid bacteria, Bacillus subtilis, and mixtures thereof, It is interpreted to be included in the extract.

In addition, the moringa extract of the present invention includes extracts obtained by using a pulping method. Fojera is a pharmaceutical technology that changes the original properties of medicines by processing the medicines based on the oriental medicine theory. Mainly refers to the process of steaming, boiling, roasting, roasting, burning, or mixing processes.

According to a preferred embodiment of the present invention, the outpost of Moringa, for example, the fruit, leaves, stems and roots of Moringa, is then directly extracted by a pure compression extraction method, and the leaves, stem and roots are squeezed by a high- Or by centrifugation.

More specifically, the Moringa extract is prepared by the following method. First, the fruit (seed) of Moringa was washed with purified water and then steamed at about 90 ° C for about 15 minutes to 1 hour. Then, it was pressed into a high-speed press to obtain an extract of the oil component, and the leaves, stems and roots were finely crushed , Steamed at 70 to 80 ° C for 30 minutes to 2 hours, then placed in a high-speed press and compressed to obtain a water-soluble extract. The above-mentioned Moringa fruit squeeze extract, leaf, stem and root squeeze extracts are mixed with each other, ethanol is added in a ratio of 1/5 to 1/20 of the total volume of the extract, and the mixture is refluxed for 30 minutes to 48 hours, Stir or stand and centrifuge or filter to remove any debris. The reflux, stirring or post-separation process is added 2-3 more times as needed. Thereafter, the weight loss is reduced to 5% to 15% (w / v), and 1 to 75% of the weight of the final extract is homogenized to prepare a colloidal composition of the present invention. In the above, the support includes an extract dissolution support such as maltodextrin, lactose, silica or any other cosmetically acceptable extract, for example, propylene glycol / ethoxylated oleo alcohol in various proportions.

The composition of the present invention further contains, as an active ingredient of the present invention, elastin, collagen and hyaluronic acid which are extracellular matrix components constituting the skin, in addition to the above-mentioned Moringa extract.

According to a preferred embodiment of the present invention, the content of Moringa extract, elastin, collagen and hyaluronic acid is 10 to 50 parts by weight, 5 to 20 parts by weight, 2 to 15 parts by weight and 5 to 20 parts by weight, Preferably 22 to 50 parts by weight, 10 to 15 parts by weight, 5 to 10 parts by weight and 10 to 15 parts by weight, respectively. When the content of the above-mentioned Moringa extract, elastin, collagen and hyaluronic acid is less than the above-mentioned respective parts by weight, the skin-cosmetic effect is not sufficiently exhibited or the synergy effect is insufficient, and there is a serious problem in formation of nanoemulsion formulations. In addition, even if the weight of each component exceeds the above-mentioned weight, there is a problem in stability of the composition for forming the nano emulsion.

According to a specific embodiment of the present invention, the cosmetic composition of the present invention comprises 22 to 50 parts by weight of moringa extract, 10 to 15 parts by weight of elastin, 5 to 10 parts by weight of collagen, 10 to 15 parts by weight of hyaluronic acid, , 5 to 10 parts by weight of tocopherol, 4 to 17 parts by weight of an emulsifier, 6 to 10 parts by weight of an alkanolamide, 8 to 10 parts by weight of trollamine and 2 to 7 parts by weight of an additive. Wherein the organic solvent comprises 15 to 26 parts by weight of one selected from the group consisting of C ₁ to C ₄ alcohols, acetone, water / alcohol mixture and isopropyl alcohol, 2 to 8 parts by weight of polyethoxy alcohol and 5 to 26 parts by weight of stearyl alcohol Wherein the emulsifier comprises 1 to 5 parts by weight of propylene glycol and 3 to 12 parts by weight of cryoglyceide, wherein the additive comprises 0.5 to 2 parts by weight of sodium tripolyphosphate, 1 to 3 parts by weight of sodium hydroxide and 0.5 to 5 parts by weight of sodium borate To 2 parts by weight.

According to a preferred embodiment of the present invention, the composition of the present invention follows the following production method.

First, the Moringa extract extracted by the extraction method of the present invention is heated to about 80 to 90 캜 and prepared to be mixed as a liquid raw material. Next, alkanolamide, elastin, collagen and hyaluronic acid were added to the liquid Moringa extract, tocopherol and an emulsifier were added as a preservative, and the mixture was stirred at a high speed of 3,000 rpm for about 15 minutes in a homomixer to obtain a homogeneous emulsion &Lt; / RTI &gt; At this time, it is preferable that a vacuum system is added so that the inside of the homomixer can be vacuum (about 10 ^-3 to 10 ^-4 mmHg). By stirring, the triglyceride and the fatty acid are separated into alkyl groups and are produced in the form of emulsions having a particle size of 0.4 μm or less. At this time, polyhydric alcohols having a carbon number of C ₁₀ to C ₁₄ are added to prepare a suspension to prevent solidification of triglyceride and fatty acid components of the moringa extract. Here, as the emulsifier, propylene glycol and triglyceride of benzyl isothiocyanate are used. Next, the emulsion mixture is cooled to room temperature. The organic solvent boils at about 80 ° C due to its characteristics and is highly volatile, so that the operation at room temperature is safe and efficient. Next, an organic solvent is added to the emulsion phase mixture at room temperature and stirred at a high speed to prepare a creamy or mucilage-like colloid. When the organic solvent is added to the emulsion mixture and stirred in a homomixer at a high speed of 3,000 rpm or more for about 10 minutes, a creamy or mucilage colloid is produced according to the composition ratio of the composition. At this time, the vacuum state can be maintained at the time of stirring. Here, the organic solvent includes isopropyl alcohol and stearyl alcohol and / or polyethoxy alcohol. At this time, the creamy or mucilage colloid in which the alkyl group is separated by the organic solvent becomes a nano-sized colloid having a particle size smaller than the size of the skin cells and having a size of 0.1 탆 or less which is easy to penetrate into the skin cells. Finally, a creamy or mucilage colloid of trollamine and an additive is added to prepare a water-soluble nano-emulsion natural cosmetic cosmetic composition.

At this time, the organic solvent is used in the range of 22 to 60 parts by weight and is added for the purpose of separating the alkyl group (Alkyl group) from the triglyceride fatty acid. Such organic solvents include, but are not limited to, higher alcohols or polyhydric alcohols such as isopropyl alcohol, polyethoxy alcohol and stearyl alcohol. Most preferably, each component of the organic solvent is comprised of 15 to 26 parts by weight of isopropyl alcohol, 2 to 8 parts by weight of polyethoxy alcohol, and 5 to 26 parts by weight of stearyl alcohol. Fatty acids in which alkyl groups are separated exist in the form of monomers. Fatty acids made of such monomers are emulsified in a homomixer, and the particle size is reduced to about 0.4 탆 or less. The alkanolamide is used in the range of 6 to 10 parts by weight, and the alkanolamide is an auxiliary surfactant for accelerating the condensation reaction with the fatty acid and functions as a nonionic surfactant. In addition, the emulsifier serves to emulsify the fatty acid and the alkanolamide, and the emulsifier as an example of the present invention includes propylene glycol and triglyceride. It is not limited thereto. Herein, 3 to 12 parts by weight of triglyceride in the emulsifier is used to accelerate the saponification reaction, thereby shortening the production time of the product. Also, propylene glycol is used in a range of 1 to 5 parts by weight. (Eg, butylparaben, propylparaben), which have problems causing skin irritation, allergies, atopic dermatitis, and the like. However, tocopherol Is used. The tocopherol is used in the range of 5 to 10 parts by weight. And 8 to 10 parts by weight of trollamine are mixed and used in the composition of the present invention. Here, the trolamine serves to promote the saponification reaction. The additive functions to control the saponification reaction of the fatty acid, and sodium tripolyphosphate, sodium hydroxide, and sodium borate are used as additives as an example of the present invention. Here, the additive comprises 0.5 to 2 parts by weight of sodium tripolyphosphate, 1 to 3 parts by weight of sodium hydroxide, and 0.5 to 2 parts by weight of sodium borate. In addition, the colloidal water-soluble natural detergent composition according to an embodiment of the present invention can achieve diversity of applications through controlling moisture and pH, and when pH control is required, citric acid or organic amine can be added.

In addition, the colloidization of the composition of the present invention can be carried out by sedimentation using sediment gravity to obtain a crystalline or amorphous dense precipitate. The method itself is simple, but in addition to gravity, complex forces such as Brownian motion, nucleation and growth due to order-disorder phase transition coexist, so colloidal crystals can be obtained by completely controlling colloid size, density and sedimentation rate. When spherical particles are used and the settling velocity becomes sufficiently low, a crystal lattice having a long-range regularity is formed on the bottom of the vessel through phase transformation. If the particle size is less than 100 nm or the density of the particles is nearly equal to the density of the dispersion medium, the dispersion is balanced. Since the density of the amorphous silica is higher than that of the polymer, it is suitable for the sedimentation method. The crystal structure is mainly a wheat cube structure (ccp).

The disadvantage of this method is that it requires a long time of several weeks or more, it is difficult to control the number of layers included in the three-dimensional lattice, the irregular structure is likely to appear in the gravitational direction, and the most important point is to precipitate into crystal or amorphous structure It is easy. Such a problem is partially solved by using a centrifugal force or an electric field to increase the sedimentation velocity or by increasing the crystallinity by applying vibration.

  The water-soluble nano emulsion cosmetic composition of the present invention produced by such a method can prevent skin irritation due to the absence of preservatives, exhibit excellent effects in soothing, cleansing, moisturizing, wrinkle and skin aging prevention, It is smaller than cell size and easy penetration into skin.

The ingredients contained in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients. For example, conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, vitamins, .

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a flexible lotion (skin), a nutritional lotion (milk lotion), a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder .

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component have.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

<Examples>

Preparation of materials and manufacturing equipment

A homomixer was used as a stirrer, and more specifically, a vacuum homo mixer was used. Such a homomixer was designed to maintain a vacuum state in order to suppress bubbles which may occur during stirring.

The stirrer specifications were set as follows.

- Homo or Ultra 3,600 rpm

- Paddle agitator 750 rpm

- Scraper paddle 50 rpm

The homomixer is capable of performing vacuum, heating and cooling functions, and the paddle agitator and the scraper papers are auxiliary equipment installed in the homomixer. In order to solve the problem that the agitated product is not sufficiently stirred when the product to be stirred is highly viscous, As a result, such a paddle agitator and scraper papers can be simultaneously used at high speed stirring to produce a product having a uniform particle size.

Production Example 1. Preparation of Moringa fruit (seed) extract

First, the fruit (seed) of Moringa was washed with purified water, and then steamed at about 90 ° C for about 15 minutes to 1 hour, and then put into a high-speed press and compressed to obtain an oil component extract. Ethanol of 1/5 to 1/20 of the total volume of the extract was added to the above Moringa fruit squeeze extract. Thereafter, the mixture was refluxed for 30 minutes to 48 hours, stirred or allowed to stand at room temperature, centrifuged or filtered to separate the residue. This refluxing, agitation or post-separation was carried out three more times and then concentrated to a dry weight loss of 5% to 15% (w / v). 1 to 75% of propylene glycol / ethoxylated oleic alcohol based on the weight of the final extract was added and homogenized to prepare a moringa fruit extract of oil phase.

Production Example 2. Production of Moringa leaf, stem and root extract (2)

First, the leaves, stems and roots of Moringa were finely crushed and then steamed at 70 ~ 80 ℃ for 30 minutes ~ 2 hours, then put into a high speed press and squeezed to obtain a water-soluble extract. To the above Moringa leaf, stem and root squeeze extract, ethanol of 1/5 to 1/20 of the total volume of the extract was added. Thereafter, the mixture was refluxed for 30 minutes to 48 hours, stirred or allowed to stand at room temperature, centrifuged or filtered to separate the residue. This refluxing, agitation or post-separation was carried out three more times and then concentrated to a dry weight loss of 5% to 15% (w / v). 1 ~ 75% of propylene glycol / ethoxylated oleo alcohol was added to the weight of the final extract to homogenize to prepare liquid moringa leaves, stem and root extracts.

Production Example 3. Preparation of Moringa mixed extract (3)

First, the fruit (seed) of Moringa was washed with purified water, and then steamed at about 90 ° C for about 15 minutes to 1 hour. Then, it was pressed into a high-speed press to obtain an oil component extract, and the leaves, Then, it was steamed at 70 to 80 ° C for 30 minutes to 2 hours, and then put into a high-speed press and compressed to obtain a water-soluble extract. The Moringa fruit squeeze extract, leaf, stem and root squeeze extracts were mixed with each other, and ethanol of 1/5 to 1/20 of the total volume of the extract was added. Thereafter, the mixture was refluxed for 30 minutes to 48 hours, stirred or allowed to stand at room temperature, and then centrifuged or filtered to separate the residue. This refluxing, agitation or post-separation was carried out three more times and then concentrated to a dry weight loss of 5% to 15% (w / v). 1 to 75% of propylene glycol / ethoxylated oleo alcohol was added to the weight of the final extract, and homogenized to prepare a Moringa mixed extract.

Example 1: Preparation of the natural cosmetic composition of the present invention (1)

40 parts by weight of the Moringa fruit extract of Preparation Example 1 was added to a high-speed homomixer, and the mixture was heated to about 80 to 100 DEG C to make it liquid. 6 parts by weight of alkanolamide, 4 parts by weight of propylene glycol, 5 parts by weight of triglyceride, , 10 parts by weight of elastin, 10 parts by weight of collagen and 20 parts by weight of hyaluronic acid were mixed and stirred in a vacuum state (about 10 ^-3 to 10 ^-4 mmHg) at a rotation speed of 3,000 rpm for about 15 minutes, Lt; / RTI &gt; particle size. The resulting product had a viscosity of about 10,000 cPs. And then to start the cooling unit to normalize the organic solvent is isopropyl alcohol, 18 parts by weight of stearyl alcohol into 7 parts by weight of a polyethoxy alcohol 3 parts by weight of a room temperature (about 24 ℃) and vacuum (10 ^-3 to 10 ^-4 mmHg), the homomixer was agitated at a rotation speed of 3,000 rpm for about 10 minutes. At this time, the resulting product was in the form of a nano emulsion having a particle size of 0.1 탆 or less. As a final step, 0.5 part by weight of sodium borate, 1.5 parts by weight of a homomixer at a rotational speed of 3,000 rpm at room temperature (about 24 ° C) and in a vacuum state (about 10 ^-3 to 10 ^-4 mmHg) by adding 1.5 parts by weight of sodium hydroxide, 2 parts by weight of sodium hydroxide and 8 parts by weight of trollamine, And the resulting mixture was saponified with stirring for a minute to finally prepare a natural cosmetic composition containing a creamy water-soluble moringa extract. The finally produced product has a particle size of 0.1 탆 or less, pH 6.5, viscosity of about 7,000 cPs.

Example 2: Preparation of the cosmetic composition for natural cleansing of the present invention (2)

50 parts by weight of the Moringa mixed extract of Preparation Example 3 was added to a high-speed homomixer, and the mixture was heated to about 80 to 90 DEG C to make it liquid. Then, 6 parts by weight of alkanolamide, 4 parts by weight of propylene glycol, 5 parts by weight of triglyceride, , 10 parts by weight of elastin, 10 parts by weight of collagen and 20 parts by weight of hyaluronic acid were mixed and stirred in a vacuum state (about 10 ^-3 to 10 ^-4 mmHg) at a rotation speed of 3,000 rpm for about 15 minutes, Lt; / RTI &gt; particle size. The resulting product had a viscosity of about 10,000 cPs. And then to start the cooling unit to normalize the organic solvent is isopropyl alcohol, 18 parts by weight of stearyl alcohol into 7 parts by weight of a polyethoxy alcohol 3 parts by weight of a room temperature (about 24 ℃), a vacuum (about 10 ^-3 to 10 ^-4 mmHg), the homomixer was stirred at a rotation speed of 3,000 rpm for about 10 minutes. The resulting product was in the form of a nano emulsion having a particle size of 0.1 탆 or less. As a final step, 0.5 part by weight of sodium borate, 1.5 parts by weight of sodium tripolyphosphate 2 parts by weight of sodium hydroxide and 8 parts by weight of trollamine were added to the homomixer at a room temperature (about 24 ° C) and a vacuum state (about 10 ^-3 to 10 ^-4 mmHg) at a rotation speed of 3,000 rpm for about 10 minutes Liver was stirred and saponified to finally produce a natural cosmetic composition containing a creamy water-soluble moringa extract. The finally produced product has a particle size of 0.1 탆 or less, pH 6.5, viscosity of about 7,000 cPs.

Example 3: Preparation of the natural cosmetic composition of the present invention (3)

12 parts by weight of Moringa fruit (seed) extract of Preparation Example 1, 3 parts by weight of Moringa liquid extract of Preparation Example 2, and 15 parts by weight of water were added to a high-speed homomixer, and the mixture was heated to about 80 to 90 DEG C to form a liquid state. 6 parts by weight of nanolamide, 2 parts by weight of propylene glycol, 5 parts by weight of tocopherol, 10 parts by weight of triglyceride, 15 parts by weight of elastin, 10 parts by weight of collagen and 20 parts by weight of hyaluronic acid were placed in a vacuum state (about 10 ^-3 to 10 ^-4 mmHg ), The homomixer is agitated at a rotation speed of 3,000 rpm for about 15 minutes to form an emulsion having a particle size of 0.4 μm or less. The viscosity of the resulting product is about 5,000 cPs. 24 parts by weight of isopropyl alcohol as an organic solvent, 24 parts by weight of stearyl alcohol and 6 parts by weight of polyethoxy alcohol were placed in a nitrogen atmosphere at room temperature (about 24 ° C) and vacuum (about 10 ^-3 ~ 10 ^-4 mmHg), the homomixer is agitated at a rotation speed of 3,000 rpm for about 10 minutes. The resulting product was made in the form of a nano-emulsion having a particle size of 0.1 탆 or less. (About 24 ° C) and a vacuum state (about 10 ^-3 to 10 ^-4 mmHg) by adding 0.5 part by weight of sodium borate, 1 part by weight of sodium tripolyphosphate, 1.5 parts by weight of sodium hydroxide, and 8 parts by weight of trollamine, , The homomixer is agitated at a rotation speed of 3,000 rpm for about 10 minutes to saponify, and finally a mucous-phase natural cosmetic composition is completed. The final product has a particle size of 0.1 탆 or less, a pH of 6.5, and a viscosity of about 2,000 cPs.

Comparative Example 1. Preparation of a cosmetic composition containing moringa extract

22 parts by weight of Moringa fruit extract of Preparation Example 1 was added to a high-speed homomixer, and the mixture was heated to about 80 to 90 DEG C to make it liquid. Then, 6 parts by weight of alkanolamide, 2 parts by weight of propylene glycol, 10 parts by weight of triglyceride, And the homomixer is agitated in a vacuum state (about 10 ^-3 to 10 ^-4 mmHg) at a rotation speed of 3,000 rpm for about 15 minutes to form an emulsion having a particle size of 0.4 μm or less. The viscosity of the resulting product is about 5,000 cPs. And then to start the cooling unit to normalize the organic solvent is isopropyl alcohol, 24 parts by weight of stearyl alcohol into 24 parts by weight of poly-ethoxy alcohol 6 parts by weight of a room temperature (about 24 ℃), a vacuum (about 10 ^-3 to 10 ^-4 mmHg), the homomixer is agitated at a rotation speed of 3,000 rpm for about 10 minutes. At this time, the resulting product is in the form of a nano-emulsion having a particle size of 0.1 탆 or less. (About 24 ° C), a vacuum state (about 10 ^-3 to 10 ^-4 mmHg), and the like, as a final step, by adding 0.5 parts by weight of sodium borate, 1 part by weight of sodium tripolyphosphate, 1.5 parts by weight of sodium hydroxide, , The homomixer is agitated at a rotation speed of 3,000 rpm for about 10 minutes and then saponified to finally obtain a mucous-colloidal water-soluble natural cosmetic composition. The final product has a particle size of 0.1 탆 or less, a pH of 6.5, and a viscosity of about 2,000 cPs.

Comparative Example 2. Preparation of Moringa extract, elastin, and hyaluronic acid-containing cosmetic composition

12 parts by weight of Moringa fruit extract of Preparation Example 1 was added to a high speed homomixer, and the mixture was heated to about 80 to 100 DEG C to make it liquid. Then, 6 parts by weight of alkanolamide, 2 parts by weight of propylene glycol, 10 parts by weight of triglyceride, 15 parts by weight of elastin and 20 parts by weight of hyaluronic acid were charged and stirred in a vacuum state (about 10 ^-3 to 10 ^-4 mmHg) at a rotation speed of 3,000 rpm for about 15 minutes to form an emulsion having a particle size of 0.4 μm or less . The viscosity of the resulting product is about 5,000 cPs. And then to start the cooling unit to normalize the organic solvent is isopropyl alcohol, 24 parts by weight of stearyl alcohol into 24 parts by weight of poly-ethoxy alcohol 6 parts by weight of a room temperature (about 24 ℃), a vacuum (about 10 ^-3 to 10 ^-4 mmHg), the homomixer is agitated at a rotation speed of 3,000 rpm for about 10 minutes. At this time, the resulting product is in the form of a nano-emulsion having a particle size of 0.1 탆 or less. (About 24 ° C), a vacuum state (about 10 ^-3 to 10 ^-4 mmHg), and the like, as a final step, by adding 0.5 parts by weight of sodium borate, 1 part by weight of sodium tripolyphosphate, 1.5 parts by weight of sodium hydroxide, , The homomixer is agitated at a rotation speed of 3,000 rpm for about 10 minutes and then saponified to finally obtain a meringue extract of the mucous phase and a cosmetic composition containing elastin and hyaluronic acid. The final product has a particle size of 0.1 탆 or less, a pH of 6.5, and a viscosity of about 2,000 cPs.

Comparative Example 3. Preparation of Moringa extract, collagen and elastin-containing cosmetic composition

12 parts by weight of Moringa fruit extract of Preparation Example 1 was added to a high-speed homomixer, and the mixture was heated to about 80 to 90 DEG C to make it liquid. 6 parts by weight of alkanolamide, 2 parts by weight of propylene glycol, 10 parts by weight of triglyceride, 10 parts by weight of elastin and 10 parts by weight of collagen were added and the homomixer was stirred in a vacuum state (about 10 ^-3 to 10 ^-4 mmHg) at a rotation speed of 3,000 rpm for about 15 minutes to form an emulsion having a particle size of 0.4 μm or less I make it. The viscosity of the resulting product is about 5,000 cPs. 24 parts by weight of isopropyl alcohol as an organic solvent, 24 parts by weight of stearyl alcohol and 6 parts by weight of polyethoxy alcohol were placed in a nitrogen atmosphere at room temperature (about 24 ° C) and vacuum (about 10 ^-3 to 10 ^-4 mmHg), the homomixer is agitated at a rotation speed of 3,000 rpm for about 10 minutes. At this time, the resulting product is in the form of a nano-emulsion having a particle size of 0.1 탆 or less. (About 24 ° C), a vacuum state (about 10 ^-3 to 10 ^-4 mmHg), and the like, as a final step, by adding 0.5 parts by weight of sodium borate, 1 part by weight of sodium tripolyphosphate, 1.5 parts by weight of sodium hydroxide, , The homomixer is agitated at a rotation speed of 3,000 rpm for about 10 minutes and then saponified to finally obtain a meringue extract of the mucous phase and a cosmetic composition containing elastin and collagen. The final product has a particle size of 0.1 탆 or less, a pH of 6.5, and a viscosity of about 2,000 cPs.

Comparative Example 4 Preparation of Soybean Oil-Containing Cosmetic Composition

12 parts by weight of soybean oil (soybean oil) extracted by pressing edible beans instead of Moringa extract was added to a high-speed homomixer, and the mixture was heated to about 80 to 90 ° C to be liquid. Then, 6 parts by weight of alkanolamide, 2 parts by weight of propylene glycol, , 10 parts by weight of tocopherol, 10 parts by weight of elastin, 10 parts by weight of collagen and 20 parts by weight of hyaluronic acid were placed in a vacuum state (about 10 ^-3 to 10 ^-4 mmHg) for about 15 minutes at a rotation rate of 3,000 rpm Followed by stirring to form an emulsion having a particle size of 0.4 mu m or less. The viscosity of the resulting product is about 5,000 cPs. 24 parts by weight of isopropyl alcohol as an organic solvent, 24 parts by weight of stearyl alcohol and 6 parts by weight of polyethoxy alcohol were placed in a nitrogen atmosphere at room temperature (about 24 ° C) and vacuum (about 10 ^-3 to 10 ^-4 mmHg), the homomixer is agitated at a rotation speed of 3,000 rpm for about 10 minutes. At this time, the resulting product is in the form of a nano-emulsion having a particle size of 0.1 탆 or less. (About 24 ° C), a vacuum state (about 10 ^-3 to 10 ^-4 mmHg), and the like, as a final step, by adding 0.5 parts by weight of sodium borate, 1 part by weight of sodium tripolyphosphate, 1.5 parts by weight of sodium hydroxide, , The homomixer was agitated at a rotation speed of 3,000 rpm for about 10 minutes and then saponified to finally obtain a mucilage-containing cosmetic composition containing soybean oil. The final product has a particle size of 0.1 탆 or less, a pH of 6.5, and a viscosity of about 2,000 cPs.

Experimental Example 1. Moisturizing Effect of Cosmetic

The clinical moisturizing effects of the cosmetic compositions of Examples 1 to 3 and Comparative Examples 1 to 4 were measured as follows. A, B, and C groups were divided into eight groups (A, B, C, D, E, F, and G) The cosmetic composition compositions of Comparative Examples 1, 2, 3 and 4 were applied to the D, E, F, and G groups, respectively, twice a day for 8 weeks, followed by washing with water. The moisturizing effect was evaluated by Corneometer CM 820 (Corage + Khazaka, Germany) for every 2 weeks and after the start of the test. The experimental results are shown in Table 1 below.

Moisturizing effect division Before use After 2 weeks of use After 4 weeks of use Example 1
(Moringa fruit extract / collagen, elastin, containing hyaluronic acid) 23.6 36.5 45.1 Example 2
(Moringa mixed extract / collagen, elastin, containing hyaluronic acid) 23.8 38.1 51.9 Example 3
(Moringa mixed extract / collagen, elastin, containing hyaluronic acid) 24.1 38.9 49.8 Comparative Example 1
(Containing Moringa fruit extract) 24.3 37.1 45.9 Comparative Example 2
(Moringa fruit extract / elastin, containing hyaluronic acid 23.5 35.3 46.7 Comparative Example 3
(Moringa fruit extract / collagen, containing elastin) 23.5 35.3 47.6 Comparative Example 4
(Containing soybean oil / collagen, elastin, hyaluronic acid) 23.7 24.1 23.9

As shown in Table 1, the cosmetic composition containing the moringa extract showed a considerable moisturizing effect compared to the cosmetic composition containing the soybean oil (Comparative Example 4) instead of the moringa extract. In particular, the cosmetic composition (Example 2 and Example 3) prepared so as to contain all the collagen, elastin and hyaluronic acid in the MORINGA mixed extract had the best moisturizing effect, and in Example 1 , Example 2 (containing 50 parts by weight) and Example 3 (containing 25 parts by weight), which contained a mixture of Moringa fruit extract, leaf, stem and root extract, had higher moisturizing effect.

Experimental Example 2. Sensory Evaluation

The sensory evaluation of the liquid soap of the present invention was carried out. Each evaluation was evaluated by the actual use test for the experimenter. Thirty women (male and female, 20 to 35 years old) were used to wash the liquid soap of Example 3 in the morning and evening. The experimental period was performed for one month, and then the cosmetic effects such as washing, skin irritation, moisturizing (moistness and softness), wrinkle improvement, and overall feeling of use were evaluated based on visual and user evaluations. Respectively. The degree of the effect was evaluated as <excellent effect / excellent / similar / no effect> than general cosmetic soap.

Sensory evaluation of the natural beauty liquid soap of the present invention Beauty effect Very good Great Similar effects no effect Cleaning effect 19 One Skin irritation or soothing effect 18 2 Skin moisturizing effect 18 2 Wrinkle improvement effect 17 2 One Overall effect 19 One

Claims (3)

10 to 15 parts by weight of collagen, 10 to 15 parts by weight of hyaluronic acid, 22 to 60 parts by weight of an organic solvent, 4 to 17 parts by weight of an emulsifying agent, 5 to 10 parts by weight of an emulsifier, 6 to 10 parts by weight of an alkanolamide, 8 to 10 parts by weight of a trollamine and 2 to 7 parts by weight of an additive as a nanoemulsion natural cosmetic composition,
The organic solvent is selected from the group consisting of 15 to 26 parts by weight of at least one selected from the group consisting of C ₁ to C ₄ alcohols, acetone, water / alcohol mixture and isopropyl alcohol, 2 to 8 parts by weight of polyethoxy alcohol and 5 to 26 parts by weight of stearyl alcohol Wherein the emulsifier comprises 1 to 5 parts by weight of propylene glycol and 3 to 12 parts by weight of triglyceride, wherein the additive is 0.5 to 2 parts by weight of sodium tripolyphosphate, 1 to 3 parts by weight of sodium hydroxide and 0.5 to 2 parts by weight of sodium borate The nanoemulsion natural cosmetic composition of the present invention. The nano-emulsion natural cosmetic composition according to claim 1, wherein the moringa extract is extracted by pressing steamed fruits, leaves, stems and roots of Moringa. A first step of adding 22 to 50 parts by weight of moringa extract to a container equipped with a homomixer and heating the mixture to about 80 to 90 캜; The method according to any one of claims 1 to 5, wherein the moringa extract of the first step contains 6 to 10 parts by weight of alkanolamide, 10 to 15 parts by weight of elastin, 5 to 10 parts by weight of collagen, 10 to 15 parts by weight of hyaluronic acid, 5-10 parts by weight of tocopherol, 17 parts by weight, and the mixture is stirred at 3000 to 5000 rpm for about 15 minutes at a high speed to prepare a homogeneous emulsion phase mixture; A third step of cooling the emulsion phase mixture to a room temperature state; Adding 22 to 60 parts by weight of an organic solvent to the mixture of the third step and stirring the mixture at 3000 to 5000 rpm for about 10 minutes at a high speed to produce a cream or mucus-like nanocolloid; And 8 to 10 parts by weight of trollamine and 2 to 7 parts by weight of an additive are added to the cream or mucilage nano-colloid to prepare a water-soluble nanoemulsion. The nano-emulsion natural cosmetic composition containing moringa extract Way.