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KR20140131150A - Pseudo pollutant containing high content of lipid derived of microalgae - Google Patents

Pseudo pollutant containing high content of lipid derived of microalgae Download PDF

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Publication number
KR20140131150A
KR20140131150A KR1020130050132A KR20130050132A KR20140131150A KR 20140131150 A KR20140131150 A KR 20140131150A KR 1020130050132 A KR1020130050132 A KR 1020130050132A KR 20130050132 A KR20130050132 A KR 20130050132A KR 20140131150 A KR20140131150 A KR 20140131150A
Authority
KR
South Korea
Prior art keywords
microalgae
lipid
pseudo
active ingredient
present
Prior art date
Application number
KR1020130050132A
Other languages
Korean (ko)
Inventor
한종인
성민아
정재인
Original Assignee
한국과학기술원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국과학기술원 filed Critical 한국과학기술원
Priority to KR1020130050132A priority Critical patent/KR20140131150A/en
Publication of KR20140131150A publication Critical patent/KR20140131150A/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00Production of fats or fatty oils from raw materials
    • C11B1/10Production of fats or fatty oils from raw materials by extracting
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a pseudo pollutant containing lipid derived from microalgae as an active ingredient. More specifically, the present invention provides a pseudo pollutant containing lipid having similar physical properties to crude oil or oil from microalgae, which is able to be conveniently cultivated within a short period, as an active ingredient for the marine pollution prevention training.

Description

[0001] The present invention relates to a virtual pollutant containing microalgae lipid as an active ingredient,

The present invention relates to a virtual contaminant comprising microalgae lipid as an active ingredient, and more particularly to a method for cultivating microalgae comprising culturing a microalgae, concentrating the microalgae to be cultured, and extracting lipids from the concentrated microalgae A method for producing microalgae lipids, a virtual pollutant containing microalgae lipid produced by the above method as an active ingredient, and a method for producing the same.

As a photoautotroph, microalgae are photosynthetic organisms that make the sun the sole source of energy and carbon dioxide as the only carbon source and have the advantage of higher growth rate than other photosynthetic organisms, especially terrestrial organisms. High productivity. Generally, microalgae exhibit a characteristic that the amount thereof is doubled within 24 hours.

Microalgae live in the marine environment and are the lowest in the marine ecosystem and are used primarily as food for fish fry. Microalgae Biomass is rich in nutrients such as protein, carbohydrates, vitamins and minerals and contains lipids that are essential for the growth of fish fry. In fact, many fish farms operate a micro-algae cultivation field around fish farms for use as rice for chewing.

In order to prepare for oil spill accidents, control training is being carried out globally by using virtual contaminants having properties similar to those of oil. On the other hand, virtual contaminants are floating, mobile oil-like substances that must float on the surface for at least 2 hours and should be easily identifiable. In addition, virtual contaminants, which are frequently used in a wide range of categories, should be characterized by low cost.

On the other hand, virtual contaminants are liquid virtual materials and solid virtual materials. In the past, substances such as popcorn, orange, and rice bran have been used, but they are known to have a bad influence on marine resources utilization. Research is underway to develop alternative liquid materials for liquids. On the other hand, U.S. Patent Nos. 7,528,097 and 7,691,908 disclose a technique for producing biodegradable non-emulsion-like crude oil derived from natural materials such as canola oil, cottonseed oil, soybean oil and sunflower oil in detail, Natural materials have a disadvantage in that the supply is not constant, and there is a problem that mass production is difficult. In addition, biodegradable non-emulsion-like crude oil derived from such a natural substance may be contrary to the Marine Environment Management Act.

In order to solve the above problems, the present invention relates to a virtual pollutant containing microalgae lipid as an active ingredient.

In order to achieve the above object, the present invention provides a virtual pollutant containing microalgae lipid as an active ingredient.

In addition, the microalgae lipid may be obtained by (a) culturing microalgae; (B) concentration of the cultured microalgae; And (c) extracting lipids from the concentrated microalgae.

In addition, the present invention provides a method for producing a virtual contaminant comprising microalgae lipid as an active ingredient.

The virtual contaminants containing the microalgae lipid as an active ingredient of the present invention can be recovered from microalgae capable of mass culture and production, and lipids having physical properties similar to those of crude oil or oil can be easily supplied and received. In addition, the present invention can use lipid components contained in microalgae for marine control training.

Hereinafter, the present invention will be described in detail.

The present invention provides a virtual contaminant comprising an algae lipid as an active ingredient.

The present invention also provides a method for producing a virtual contaminant comprising microalgae lipid as an active ingredient.

The microalgae are selected from strains which have a high lipid content and are capable of mass culture and production so as to meet the object of the present invention and are selected from the group consisting of Chlorella sp., Rhodotorula sp., Scenedesmus sp. two anri Ella (Duanliella sp.), nanno keulroriseu (Nannochloris sp.), bidul Pia (Biddulphia sp.), Kane Chitose Ross (Chaetoceros sp.), mono Douce (Monodus sp.), in Paris, Saturday keulroriseu (Parietochloris sp .), Emilia California (Emiliania sp.), isobutyl Cri sheath (Isochrysis sp.), L Pseudomonas (Phaeomonas sp.), glow predetermined mask matrix (Glossomastrix sp.), APA furnace kapsa (Aphanocapsa sp.), Spirulina (Spirulina such as Rhodotorula sp., Trichodesmium sp., Hemiselmis sp., Rhodomonas sp., Gymnodinium sp., etc., but preferably Rhodotorula sp. sp.) may be cultured, but the present invention is not limited thereto.

The microalgae lipids comprise (a) culturing microalgae; (B) concentration of the cultured microalgae; And (c) extracting lipids from the concentrated microalgae.

The step (a) may be a conventional microalgae cultivation step, but may include, but is not limited to, a raceway pond or a photobioreactor method. Carbon dioxide which is essential for photosynthesis such as photoauthotroph culture in the microalgae culture step can be supplied by a conventional method used in the art. On the other hand, a microalgae culture step can be performed by feeding a nutrient source necessary for microalgae growth such as nitrogen and phosphorus by using a heterotrophic culture method. In the step of culturing the microalgae, impurities or suspended substances may be additionally removed by filtration or coagulant.

The step (b) is a step of concentrating the microalgae cultured in step (a), wherein the concentration of the microalgae obtained by the step of culturing the microalgae is less than 1 g / L, , Flocculation, osmotic, or centrifugal separation may be used, but the present invention is not limited thereto.

The step (c) is a step of extracting lipids from the microalgae concentrated in step (b), and may be carried out using a physical method and / or a chemical method. Physical methods include disruption of microalgae cell walls using ultrasound waves such as ultrasonic waves or microwaves, selective extraction of lipid components, or decomposition of components such as proteins and carbohydrates constituting microalgae using pyrolysis Only components are selectively extracted. The chemical method may be performed by a solvent extraction method in which an organic solvent is used to selectively dissolve lipid components contained in microalgae. The organic solvent may be hexane, ether, chloroform, And a mixture thereof. Of these, hexane is preferably used. The solvent extraction method can be carried out by a conventional stirring extraction apparatus.

The step (c) may further include the step of applying pressure to the concentrated microalgae in the step (b), which may increase the efficiency of microalgae lipid extraction.

Meanwhile, the lipid may be extracted in the form of a fatty acid such as C18: 0, C18: 1, or C18: 2.

The present invention also provides a method for producing a virtual contaminant comprising microalgae lipid as an active ingredient.

The virtual contaminants containing the microalgae lipid as an active ingredient of the present invention can be used for marine control training in which the physical properties of lipids extracted from microalgae are similar to crude oil or oil.

Claims (2)

A virtual contaminant containing microalgae lipid as an active ingredient. The method according to claim 1,
The lipid may be selected from the group consisting of: (a) microalgae culture; (B) concentration of the cultured microalgae; And (c) extracting lipids from the concentrated microalgae.
KR1020130050132A 2013-05-03 2013-05-03 Pseudo pollutant containing high content of lipid derived of microalgae KR20140131150A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020130050132A KR20140131150A (en) 2013-05-03 2013-05-03 Pseudo pollutant containing high content of lipid derived of microalgae

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020130050132A KR20140131150A (en) 2013-05-03 2013-05-03 Pseudo pollutant containing high content of lipid derived of microalgae

Publications (1)

Publication Number Publication Date
KR20140131150A true KR20140131150A (en) 2014-11-12

Family

ID=52452687

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020130050132A KR20140131150A (en) 2013-05-03 2013-05-03 Pseudo pollutant containing high content of lipid derived of microalgae

Country Status (1)

Country Link
KR (1) KR20140131150A (en)

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