KR20130120597A - Composition for anti-inflammatory containing soybean extract of fermentation by flower - Google Patents

Abstract

The present invention relates to an anti-inflammatory composition containing a soybean extract or a fraction thereof as an active ingredient, particularly, to a composition containing a soybean extract or a faction thereof containing 700 ug/g of dipeptides with anti-inflammation activities. A method for preparing the soybean extract comprises the steps of heating soybeans; fermenting the heated soybeans and flowers; and extracting the fermented soybeans.

Description

COMPOSITION FOR ANTI-INFLAMMATORY CONTAINING SOYBEAN EXTRACT OF FERMENTATION BY FLOWER}

The present invention relates to an anti-inflammatory composition comprising soy extract or fractions thereof as an active ingredient, and specifically, the soy extract or fractions thereof relates to a composition comprising a dipeptide having anti-inflammatory activity.

The skin is a part of the body that is directly exposed to the external environment. Exposure to excessive ultraviolet rays, pollutants or irritants causes skin irritation and inflammatory reactions such as erythema, edema, and itching. The skin trouble caused by the skin irritation stress is not only aesthetic problem, but also the substances produced during the inflammatory reaction incidentally cause pigmentation of the skin and promote the breakdown of the skin elastic fibers to affect the increase of skin wrinkles. Known (Toxicol In Vitro. 2004; 18: 231-43, Irritant dermatitis. 1996; pp. 87-111).

Among the cosmetic ingredients, retinoids and fruit acids, which are functional ingredients used for the purpose of exfoliation, skin wrinkle improvement and prevention, are the main substances causing troubles on the skin, and there are many limitations on the use of these substances in concentrations that can exhibit maximum efficacy. (Lancet. 1994; 344: 1570-1571, Bio chem Biophys Res Commun. 1999; 255: 64-69). Therefore, in order to reduce skin irritation and inflammation caused by various irritants and to reduce side effects due to the use of cosmetics, the development of a substance or composition having anti-inflammatory and skin irritation efficacy has been a problem in the art.

The present inventors have confirmed that the extract of soybeans obtained by fermentation using flowers has anti-inflammatory effect and skin irritation-reducing effect, and completed the present invention.

Toxicol In Vitro. 2004; 18: 231-43, Irritant dermatitis. 1996; pp. 87-111 Lancet. 1994; 344: 1570-1571, Bio chem Biophys Res Commun. 1999; 255: 64-69

It is an object of the present invention to provide an anti-inflammatory composition derived from nature and a method for producing the same.

In order to achieve the above object, the present invention includes a soybean extract or a fraction thereof as an active ingredient,

The soybean extract or a fraction thereof provides an anti-inflammatory composition containing at least 700 μg / g of the dipeptide having anti-inflammatory activity with respect to the total extract or fraction weight.

In addition, the present invention is a method of producing a soybean extract for anti-inflammatory, the method

(a) applying heat to the beans;

(b) fermenting the beans with flowers; And

(c) provides a manufacturing method comprising the step of extracting the fermented soybeans.

The composition comprising the extract of Ssam fermented soybean extract of the present invention contains extremely high content of dipeptides, thereby reducing the secretion of interleukin for stimulation, and thus anti-inflammatory and skin irritation compared to general soybean extract or general soybean fermented extract. Efficacy in alleviation, and can be used in various fields such as cosmetics and medicines. In addition, since the present invention is a composition obtained by using flowers and beans, it is naturally friendly and has a low possibility of causing trouble due to low irritation to the skin.

In the present specification, the "flower" is not limited in its kind, and specifically, brown, chrysanthemum, kwanhwa, kanto, lump, gold and silver, painting, homegrown, painting, magnolia, wheat dream, windproof, balsam, pomegranate , Line drawing, line drawing, songhwa, god, softening, Wonhwa, Kwakhwa, gunhwa, phoenix and honghwahwa may be one or more selected from the group consisting of, but is not limited thereto.

Pueraria lobata is a dye plant that yields green color and can be used to medicate each part of the pan. Brown root, the root of 칡, contains isoflavones, which is effective for fever and sweating. Gall flower is a flower of 칡. It is used for headache, fever and excessive chest pain due to excessive drinking, soreness of the limbs, and thirst, anorexia, bloating, and vomiting.

Chrysanthemum ( Dendranthema grandflorum ) is a dried flower of persimmon or white soup , also called Jinguk , Geumjeong, and Cut flowers. Chrysanthemum has a fever in the chest and is effective for stuffy symptoms, pneumonia, bronchitis, headache, stiff shoulders, and high blood pressure. It is used for skin problems such as liver function, hyperemia, menstrual irregularities and acne.

Corolla is a perennial herb, Celosia , belonging to the amaranth family. cristata ) Flowers, leaves alternate, long petiole, ovate or ovate lanceolate, pointed tip, 5-10cm long, 1-3cm wide, basal bottom. Flowers bloom in July-August in red, yellow, and white, and fruits are egg-shaped, with 3-5 black seeds. Coronary artery is used for hemostatic purposes for ophthalmic diseases, hypermenstrual, uterine bleeding, and hemorrhage.

Kanto is the flower of the Asteraceae, Tussilago It is a bud of farfara ) and is also known as Kanto, vortex, vomiting, fairy tale, dongdong, chandong, takuo, and lake. Kwanhwa is used for cough, expectorant, sputum and pulmonary tuberculosis, lung abscess, and is used for external seawater, asthma, and the above-mentioned infectious disease. Kwanhwa is effective in increasing respiratory secretions, antitussive action, respiratory excitability and light anxiety, vasoconstriction, acute bronchitis in children, bronchial asthma, and sore throat.

Goehwa are paintings of trees and beans (Sophora japonica ) buds. Necrosis is used to stop constipation, abortion, and hemostasis when eyeball hyperemia, intestinal bleeding, uterine bleeding, nosebleeds, bloody stool, and blood vomit due to liver fever. It is also effective for hypertension, headache, dizziness, arteriosclerosis, stroke, cerebral hemorrhage, and stuffy chest. Hemostasis, decreased capillary permeability, lowered blood pressure, lowered cholesterol, anticonvulsion, anti-ulcer, anti-radioactivity, frostbite treatment, It has a gentle action.

Gold and Silver Coins ( Lonicera) japonica ) or buds of the same plant. Gold and silver coins are used when the heat is lowered and the chest is thirsty and thirsty. It is good for inflammation, and it causes boils, skin toxins, organ inflammation, and pus. Also used for dysentery, skin tissue necrosis due to heat poisoning, mastitis. It is applied to colitis, gastric ulcer, cystitis, sore throat, tonsillitis, bronchitis, conjunctivitis and swelling, mumps and high fever, purulent infection. Gold and silver has antibacterial, anti-inflammatory, antipyretic, leukocyte phagocytosis, central nervous system excitability, serum cholesterol lowering, ulcer prevention effect.

Drawing of the Rosaceae Peach Tree ( Prunus persica or Prunus say flowers of davidiana ). It has the ability of diuresis, blood, and stool, and treats several species, each, faeces, and dysmenorrhea.

Cactus is a flower of Hierochloe odorata , a perennial herbaceous plant belonging to the flower family. It is used to stop vomiting blood and nosebleeds and to relieve pain.

The flower is a perennial herbaceous plant belonging to the flower family, I mperata. cylindrica ) is a flower. Efficacy in bleeding, orthodoxy, hemostasis, and clear fever. It is used for the treatment of hemorrhage, nasal bleeding, and ulcers.

Magnolia is a leaf of Mallowaceae, Hibiscus syriacus ) is a flower. Magnolia cures intestinal bleeding, dysentery, whites and skin diseases.

Wheat dreams are the buds of Buddleja officinalis . The appearance is irregular conical shape and the outer surface is yellow and is covered with soft hairs. Buds are short rod-shaped, soft in quality. Mildew is used for eye redness, pain, reluctance to sunshine, tears caused by liver fever, cataracts and glaucoma. It also treats children's mama, measles, and sensitized venom in the eye.

Windproof shoes are windproof with mountain ( Saposhnikovia It is a flower of divaricata ). Windproof flowers are white and hang on the end of the main stems and the branches of the branches. The flowering period is from July to August. Windproof shoes are used for the treatment of heart pain, extremity and fracture pain.

Balsam is an annual plant of Impatiens balsamina ) is a flower. Balsam has been known to have inhibitory activity against chromophoric glucose, Pseudomonas aeruginosa, Typhoid bacillus, and Lycobacterium. It is mainly used for the treatment of urinary bleeding, post-birth blood, bruises, scabies, and intestines.

Pomegranate is a pomegranate from the pomegranate ( Punica granatum ) flowers. Pomegranate is used for hemorrhage, bleeding due to nasal bleeding, otitis media, and bleeding.

Liliaceae Inula ( Inula) It is a flower of britannica ). The abdomen circulates and releases clumps, sputum, cough, hiccups, belching, asthma, chronic bronchitis, acute pleurisy, and chest pains as if it's always full. Acupuncture has pharmacological effects such as anticonvulsant and diuretic effects of bronchus.

Daffodil is a perennial plant of buckthorn, Calystegia japonica ) is a flower. Flowers bloom in June-August, light red, with long stems on axillas, running one by one at the end. The two bracts under the calyx are green and heart-shaped. It is used to nourish the face, get rid of freckles on the face, and improve the appearance of the face.

Calendula is Pinus pine densiflora ) flowers. A male flower runs under several new branches of a male and female pine tree, elliptical, and brown. Female flower runs 1 ~ 3 at the top and is ovate about 6mm long. In addition, the pollen is characterized by a yellowish and sweet scent of light, and is mainly used to make food and smuggling, also called Songhwang.

Magnolia with Magnolia denudata ) or bud of the same plant. Shin is treating cold emotions in the body, nasal congestion, nasal congestion, runny nose, lack of smell, headache or loss of concentration, chills, fever, generalized cough and cough . The god has astringent action, capillary dilation action, anti-inflammatory action, hypotensive action, analgesic, sedation, skin fungus and staphylococcus suppressing action.

Softening is yeon with trainingNymphaea nelumbo) Is a flower. Lotus flower has the effect of bleeding, hemostasis, and moisturizing. It is used to treat bleeding and blisters caused by bruises.

The original painting refers to the buds of the Daphne genkwa . Wonhwa is used for species to shed water in asthma, sea water and flanks. Also use only for abdominal swelling, boils, head rashes, urine, and stool.

Quikhwa is mallow flower ( Althaea) rosea ) flower. Moisturization is used for white crabs, cold abdominal pain, inconspicuous pain, bleeding, uterine bleeding, burns, and red nose. It has been reported to be effective against malaria.

Olimaceae green onion ( Allium fistulosum ) flower. Appearance is umbel, flat spherical, muzzle is membranous, ovate or ovate lanceolate, 6 barks are lanceolate, white, a little shorter on outer side. Totalization is used for the treatment of abdominal pain with nasal pain.

Pohwang the units (Typha of units orientalis ) flower. The female flower is 3 ~ 10㎝ long, hangs on the upper part, and has a stem on the ovary, and the pistil is similar to the spatula. A male flower is yellow with a hair like a beard at the bottom. The pollen lowers blood fever, convergence, and hemostatic action, which is used for keratosis, hemostasis, nosebleeds, urine bleeding, and uterine bleeding, and improves blood circulation. The pharmacological action of turmeric has been reported, such as uterine excitement, lowering blood pressure, jingyeong, reduction of blood clotting time, tuberculosis bacterium growth, cholesterol lowering.

Safflower is a safflower of the Asteraceae ( Carthamus tinctorius ) flowers. The pharmacological action of safflower is known to be effective in the contraction of the uterus and intestines through the excitement of smooth muscles. Safflower is effective in the use of blood, pain, pain, pain, aches and pains to treat amenorrhea, egg delivery, stillbirth, fish blood pain, carbuncles and bruises.

In the present specification, the "bean" is not limited in its kind, and specifically, lyric ( Glycin) max ), Rhynchosia Nolubilis ), Black pollen ( Glycine) max ), Cheongtae ( Glycine max ), Emperor ( Glycine max ), Fence beans ( Vicia faba ), kidney beans ( Phaseolus vulgaris ), stained kidney beans ( Phaseolus vulgaris ), red beans ( Vigna angularis ), Soybeans ( Phaseolus angularis ), Bean sprouts ( Glycine max ) and soybean ( Glycine max ) may be one or more selected from the group consisting of, but is not limited thereto.

In one aspect, the present invention includes a soybean extract or a fraction thereof as an active ingredient, wherein the soybean extract or a fraction thereof contains at least 700 μg / g of dipeptide having anti-inflammatory activity with respect to the total extract or fraction weight, anti-inflammatory It relates to a composition for.

The “bean extract” herein may be a crude extract of water, a C ₁ -C ₆ alcohol, and a solvent selected from the group consisting of combinations thereof. The C ₁ -C ₆ alcohol may specifically be methanol or ethanol. When the soybean is extracted with a solvent, it is preferable to extract by adding a solvent corresponding to about 5 to 15 times the soybean, specifically, it is preferable to extract by adding about 10 times the solvent, but is not limited thereto. The extraction may be a heat extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction, and the like, there is no limitation if the extraction is obvious to those skilled in the art. The extraction may be performed at room temperature, but for more efficient extraction, the extraction may be performed under heating conditions, preferably at about 40 to 100 ° C., more preferably at about 80 ° C., but is not limited thereto. It is not. The extraction time is preferably about 2 to 4 hours, more preferably about 3 hours, but is not limited thereto, and may vary depending on conditions such as extraction solvent and extraction temperature. The extraction may be extracted one or more times several times in order to obtain a larger amount of the active ingredient, preferably one to five times, more preferably three times the continuous extraction can be used combined extract.

In the present specification, the soybean extract may include a crude extract of soybean as described above, and may include the crude extract as a soluble fraction of an organic solvent obtained by further extracting the organic extract with a low polarity organic solvent. Examples of the organic solvent include, but are not limited to, hexane, methylene chloride, ethyl acetate, n-butanol, and the like. The extract or the soluble fraction of the extract may be used as it is, or may be used as an extract form by concentration after filtration, and may be used as a form of a lyophilizate by concentration and freeze-drying.

As used herein, "fraction of soybean extract" refers to a component that has been separated through an additional fractionation process for soybean extract. In one embodiment, the fraction of the soybean extract is an ethyl acetate or butanol fraction for the C ₁ ~ C ₅ alcohol extract, specifically, may be an ethyl acetate fraction.

As used herein, "anti-inflammatory activity" means any kind of activity that inhibits or inhibits inflammation. For example, as a non-limiting example, the anti-inflammatory composition of one aspect of the present invention can inhibit the stimulation source by 40% or more, as compared to the experimental group that does not have any treatment on the cells secreting interleukin. In the present specification, the inhibition rate of the stimulus source is obtained by Equation 1 in the embodiment. Alternatively, the anti-inflammatory activity of the present specification means activity showing an increased secretion amount of interleukin similar to or slightly higher than that of the experimental group which did not perform any treatment on cells secreting interleukin even by stimulation of a stimulus that secretes interleukin.

In the composition of one aspect of the present invention, if the soybean extract or a fraction thereof contains less than 700 μg / g of the total weight of the extract or the fraction having anti-inflammatory activity, dramatic anti-inflammatory activity may not be sufficiently observed. In view of the above, the soybean extract or fractions thereof, the dipeptide having an anti-inflammatory activity, based on the total extract or fraction weight, 700 μg / g or more, 750 μg / g or more, 800 μg / g or more, 850 μg / g At least 900 μg / g, at least 950 μg / g, at least 1000 μg / g, at least 1050 μg / g, at least 1100 μg / g, at least 1150 μg / g, at least 1200 μg / g, at least 1250 μg / g, 1300 μg / g or more, 1350 μg / g or more, 1400 μg / g or more, 1450 μg / g or more, 1500 μg / g or more, 1550 μg / g or more, 1600 μg / g or more, 1650 μg / g or more, 1700 μg / g, 1750 µg / g or more, 1800 µg / g or more, 1850 µg / g or more, 1900 µg / g or more, 1950 µg / g or more, or 2000 µg / g or more. Specifically, the soybean extract or a fraction thereof is a dipeptide having an anti-inflammatory activity 700g / g to 9000g / g, 750g / g to 9900g / g, 800g / g to 9800 with respect to the total extract or fraction weight Μg / g, 850 μg / g to 9700 μg / g, 900 μg / g to 9600 μg / g, 950 μg / g to 9500 μg / g, 1000 μg / g to 9400 μg / g, 1050 μg / g to 9300 Μg / g, 1100 μg / g to 9200 μg / g, 1150 μg / g to 9100 μg / g, 1200 μg / g to 9000 μg / g, 1250 μg / g to 8900 μg / g, 1300 μg / g to 8800 Μg / g, 1350 μg / g to 8700 μg / g, 1400 μg / g to 8600 μg / g, 1450 μg / g to 8500 μg / g, 1500 μg / g to 8400 μg / g, 1550 μg / g to 8300 Μg / g, 1600 μg / g to 8200 μg / g, 1650 μg / g to 8100 μg / g, 1700 μg / g to 8000 μg / g, 1750 μg / g to 7900 μg / g, 1800 μg / g to 7800 It may be contained at μg / g, 1850 μg / g to 7700 μg / g, 1900 μg / g to 7600 μg / g, 1950 μg / g to 7500 μg / g or 2000 μg / g to 7400 μg / g.

An anti-inflammatory composition, which is an aspect of the present invention, induces inhibition of one or more of the expression of interleukin-6, the expression of interleukin-8, and the expression of monocyte chemoattractant protein-1, thereby alleviating skin irritation or inflammation, and also retinoication. Skin irritation or inflammation caused by acid or sodium lauryl sulfate.

In a composition of one aspect of the invention, the dipeptide of the extract or fraction may be one or more of Glu-Phe and Glu-Tyr. The weight ratio of Glu-Phe: Glu-Tyr included in the extract or fraction may be 0 to 50: 1, but is not limited thereto. Specifically, 1 to 45: 1, 1 to 40: 1, and 1 to 35: It may be included in a weight ratio of 1, 1-30: 1, 1-25: 1 or 1-20: 1.

In the composition of one aspect of the present invention, the extract or fraction may have an anti-inflammatory activity exhibiting a stimulus suppression rate of 40% or more compared to the untreated group. "Untreated group" refers to an experimental group that has not been treated with cells that secrete interleukin. If the stimulus suppression rate of more than 40% compared to the untreated group, it can be seen to have an antimicrobial activity against inflammation. In view of the above, the extract or fraction contained in the composition of the present invention is a stimulation source, 50% or more, 60% or more, 70% or more, 80% or more, 90% or more, 100% or more, 110 More than 120%, more than 130%, more than 140%, more than 150%, more than 160%, more than 170%, more than 180%, more than 190%, or more than 200% of stimulus suppression. Specifically, the extract or fraction included in the composition of one aspect of the present invention, 50 to 700%, 60 to 690%, 70 to 680%, 80 to 670%, 90 to 660%, 100 to 650 relative to the irritant %, 110-640%, 120-630%, 130-620%, 140-610%, 150-600%, 160-600%, 170-590%, 180-580%, 190-570%, 200-560 A percentage of stimulus inhibition may be indicated.

Alternatively, in the composition of one aspect of the present invention, the extract shows a similar or slightly lower increase in the amount of interleukin secretion than the experimental group without any treatment to the cells secreting the interleukin, even by stimulation of the stimulus that secretes the interleukin. . Specifically, when the extract is treated to the cells, the amount of interleukin secretion is increased by 150% or more compared with the amount of interleukin secretion of cells not treated with the stimulus even by stimulation of the stimulus that secretes interleukin. In the present specification, the “stimulatory source” includes retinoic acid or sodium lauryl sulfate.

In a composition which is one aspect of the present invention, the soybean includes fermented soybean. "Floral fermentation fermentation" of the present specification may include, for example, fermenting soybeans in flowers, fermenting soybeans in petals, or fermenting together soybeans or crushed soybeans, but is not limited thereto. No, it is meant to include all instances of "fermenting together" legumes.

In one aspect of the present invention, the extract or fraction may be contained in an amount of 0.001 to 30% by weight based on the total weight of the composition. If it is less than 0.001% by weight, the desired effect cannot be expected. If it exceeds 30% by weight, the increase of the effect is insignificant compared to the increase in content, and the formulation stability may be deteriorated. In view of the above, the extract or fraction may be contained in 0.005 to 28% by weight, 0.01 to 26% by weight, 0.05 to 24% by weight, 0.1 to 22% by weight or 0.5 to 20% by weight relative to the total weight of the composition. have.

In the composition which is one aspect of the present invention, the flower is brown, chrysanthemum, corolla, Kanto, lump, gold, silver, safflower, nacho, magnolia, wheat dream, windproof, balsam, pomegranate, scallop, daffodil, It may be one or more selected from the group consisting of songhwa, sinhwa, softened, wonhwa, quenization, sum total, sulphate and safflower.

In the composition of one aspect of the present invention, the soybean may be one or more selected from the group consisting of frosted, seomoktae, black Tae, chungtae, yellow, hedge, kidney beans, ground kidney beans, red beans, soybeans, bean sprouts and soybeans.

In one aspect of the invention, the composition may be for reducing interleukin secretion, the interleukin comprises interleukin-8 or interleukin-6.

In a composition which is an aspect of the present invention, the composition may be a pharmaceutical composition.

In one embodiment, the pharmaceutical composition may be anti-inflammatory, and may be a pharmaceutical composition effective for anti-inflammatory action involving interleukin.

When the composition according to the present invention is applied to medicines, it may be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding an inorganic or organic carrier to the composition as an active ingredient.

Examples of the agent for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. Examples of the parenteral administration agent include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method. Surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffering agents, suspending agents and other adjuvants can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously and the like.

In addition, the dosage of the active ingredient will depend on the age, sex, body weight of the subject to be treated, the particular disease or condition to be treated, the severity of the disease or condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of those skilled in the art. Typical dosages are from 0.001 mg / kg / day to 2000 mg / kg / day, more specifically from 0.5 mg / kg / day to 1500 mg / kg / day.

In one aspect of the invention, the composition may be a health food composition. Specifically, the composition may be anti-inflammatory, and may be a health food composition effective for anti-inflammatory action involving interleukin.

The composition according to the invention provides various types of food additives or functional foods comprising. It can be processed into fermented milk, cheese, yogurt, juice, probiotic and health supplement containing the composition, and can be used in the form of various other food additives.

In one embodiment, the composition may contain other ingredients and the like that can give a synergistic effect to the main effect within a range that does not impair the main effect of the present invention. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, supplementary ingredients such as water soluble vitamins, oil soluble vitamins, polymer peptides, polymer polysaccharides and seaweed extract may be further included. The above components may be mixed and selected without difficulty by those skilled in the art depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present invention. For example, the addition amount of the above components may be in the range of 0.01 to 5% by weight, more specifically 0.01 to 3% by weight, based on the total weight of the composition.

The composition according to the present invention may be in various forms such as a solution, an emulsion, a viscous mixture, a tablet, a powder, etc., and may be administered by various methods such as simple drinking, injection administration, spraying or squeezing.

In one aspect of the invention, the composition may be a cosmetic composition.

The cosmetic composition is not particularly limited in formulation, and may be appropriately selected as desired. For example, softening cream (skin lotion and milk lotion), nourishing cream, essence, nourishing cream, massage cream, pack, gel, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, It may be prepared in any one or more formulations selected from the group consisting of body lotion, body cream, body oil and body essence, but is not limited thereto. In addition, the cosmetic composition may be used in the form of an external preparation for skin in the form of ointment, patch, or the like.

The cosmetic composition according to the present invention may be provided in all formulations suitable for topical application. For example, it may be provided as a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in water, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol composition. Compositions of such formulations may be prepared according to conventional methods in the art.

The cosmetic composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, so long as they do not impair the main effect. The cosmetic composition according to the present invention may further comprise a moisturizing agent, an emollient agent, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cold agent or a limiting agent. The blending amount of the above components can be easily selected by those skilled in the art within the range that does not impair the object and effect of the present invention, the blending amount may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight based on the total weight of the composition. have.

In another aspect, the present invention provides a method for producing an anti-inflammatory bean extract, the method

(a) applying heat to the beans;

(b) fermenting the beans with flowers; And

(c) relates to a manufacturing method comprising the step of extracting the fermented soybeans.

Fermenting the beans of step (b) with the flowers in the manufacturing method includes fermenting the beans wrapped in the flowers, or fermenting the beans with petals. none.

In the production method of one aspect of the present invention, the step (b) may be a step of fermenting beans for 5 to 40 ℃ with flowers for 3 to 10 days. In the production method of one aspect of the present invention, when fermented under the above conditions, the extract of soybean may contain a large amount of dipeptide. Specifically, when the fermentation is less than 5 ℃, fermentation can not occur sufficiently, if the fermentation exceeds 40 ℃ soybean itself decay. In view of the above, the temperature of the step of fermenting soybean may be 7 ~ 38 ℃, 9 ~ 36 ℃, 11 ~ 34 ℃, 13 ~ 32 ℃ or 15 ~ 30 ℃, more specifically can be fermented at room temperature have. In addition, if fermentation is less than 3 days, fermentation does not proceed sufficiently, and if more than 10 days, soybeans decay rotten smell. In view of the above, the fermentation period may be 4-9 days, 5-8 days or 6-7 days.

In the manufacturing method of one aspect of the present invention, step (b) may be a step of fermenting beans for 3 to 10 days at room temperature with flowers.

In the production method which is an aspect of the present invention, the production method after the step of fermentation,

(b) removing the flowers from the fermented soybeans.

In the manufacturing method which is one aspect of this invention, the said fermentation includes the step of leaving beans and flowers together in a container. By leaving the beans and flowers together, the anti-inflammatory activity of the extract can be excellent.

In the manufacturing method which is one aspect of this invention, the said container contains an onggi.

In the production method of one aspect of the present invention, the flower is brown, chrysanthemum, corolla, Kanto, lump, gold and silver, flower, homegrown, flower, woody, wheat dream, windproof, balsam, pomegranate, lined, line drawing It may be one or more selected from the group consisting of, songhwa, god, softening, wonhwa, quenization, sum total, huang and safflower.

In the manufacturing method of one aspect of the present invention, the soybean may be one or more selected from the group consisting of frosted, seomoktae, blacktae, chungtae, yellow, hedge, kidney beans, ground kidney beans, red beans, soybeans, bean sprouts and soybeans. .

Hereinafter, the present invention will be described in more detail through preferred embodiments of the present invention, but the following examples and the like are intended to confirm the effects of the present invention by way of example, and the scope of the present invention is not limited thereto.

Example 1 Preparation of Black Bean Extract Fermented with Ssam using a Lotus Flower

2 liters of water was added to 1.5 kg of commercially available Seoritae and boiled at 70 ° C. for 1 hour. After boiled frosted tea, it was cooled for 1 hour at room temperature, and the cooled black beans were wrapped in 30g of lotus inside and tied to the cotton room so that the lotus did not loosen. In this way, boiled black soybeans were wrapped in lotus and the ingredients were placed in the pot and stored in the shade at room temperature for 7 days. Thereafter, after removing the lotus, black beans were collected and pulverized with a blender. Then, 7 liters of purified water was added, extracted under reflux three times, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 195 g of lotus leaf fermented black soybean extract.

Comparative Example 1 Preparation of Black Bean Extract

1.5 kg of commercially available Seoritae was pulverized with a blender, and 7 liters of purified water was added thereto, followed by extraction under reflux three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 180 g of black soybean extract.

Test Example 1 Peptide Content Analysis in Soybean Extract

About 1 g of each sample prepared in Example 1 and Comparative 1 was taken, extracted with ultrasonic waves with 60 ° C. water for 30 minutes, and dispersed well. It was then filtered through a 0.2 μm PVDF membrane filter and 1 μl of each sample solution was injected into a high performance liquid chromatography / ultraviolet absorbance photometer and analyzed. Concentration measurement results of the analysis results are shown in Table 1 below.

Dipeptide Ingredients Peptide Concentration (μg / g) Example 1 Comparative Example 1 Glu-ph 1018 265 Glu-tyr 1227 357

According to Table 1, compared to Comparative Example 1, it can be seen that the dipeptide content of Experimental Example 1 increased by three times or more.

Test Example 2 Confirmation of IL-8 Inhibitory Effect by Retinoic Acid Stimulation in Keratinocyte Line HaCaT of Anti-stimulatory Complex

Test cells, keratinocytes (Cell name: HaCaT obtained from Dr. NE Fusenig, Deutsches Krebsforschungszentrum, Heidelberg, Germany) and 10% fetal bovine serum (Fetal bovine sereum, FBS, obtained from Gibco, USA) and 1% Approximately 2 × 10 in 96 well plates using DMEM (Dulbecco's Modified Eagle Medium, Lonza, USA) medium containing Penicillin-Streptomycin (Gibco, USA) After dispensing at a density of ⁴ cells / well, 37 ° C., 5% CO ₂ Incubated for 24 hours under conditions. After the culture solution was removed, the cells were washed once with 200 ul of phosphate buffered saline (PBS), and 200 ul of DMEM medium containing fetal bovine serum was added and incubated for 24 hours. After treating with Glu-Phe, Glu-Tyr and other dipeptide substances at 0.01% by weight in DMEM medium containing 1% fetal bovine serum for 10 minutes, retinoic acid (Sigma- Aldrich, USA) and further incubated for 24 hours. Interleukin-induced retinoic acid using Interleukin-8 (IL-8), Monocyte chemoattractant protein-1 (MCP-1) ELISA kit (obtained by BD pharmigen, USA) -8 (Interleukin-8; IL-8) free inhibitory effect was measured and the results are shown in Table 2 below.

The inhibitory capacity was calculated based on the absorbance of Recombinant Interleukin-8, a standard material, to obtain a reaction formula between the absorbance and the standard material, and then substituted by the absorbance of the treated material to Interleukin-8 (Interleukin-8; The secretion amount of IL-8) was obtained. Thereafter, the stimulation relaxation rate was evaluated by Equation 1 below.

[Equation 1]

Stimulation alleviation rate (%) = 100 x [1- (Increased IL-8 secretion in the retinoic acid and substance mixture group / IL-8 secretion increased in the retinoic acid alone group)]

Test group Test concentration Stimulus Secretion increase (pg / ml) % Inhibition of stimulus IL-8 IL-8 Untreated control group 24.6 - Retinoic acid 5 uM 57.5 - Hydrocortisone 5 uM Retinoic acid 5 uM 28.9 87.1% Glu-tyr 0.01% Retinoic acid 5 uM 43.9 41.3% Glu-ph 0.01% Retinoic acid 5 uM 35.2 67.7% Val-Lys 0.01% Retinoic acid 5 uM 58.7 -3.7%

In the results of Table 2, the Glu-Tyr and Glu-Phe components at 0.01% concentration showed higher interleukin-8 secretion inhibition against retinoic acid stimulation than when Val-Lys 0.01% was treated.

Experimental Example 3 Evaluation of Inhibitory Activity of IL-6 by Sodium Lauryl Sulphate (SLS) Stimulation in Keratin Cell Line HaCaT of Anti-stimulatory Complex

Test cells, keratinocytes (Cell name: HaCaT obtained from Dr. NE Fusenig, Deutsches Krebsforschungszentrum, Heidelberg, Germany) and 10% fetal bovine serum (Fetal bovine sereum, FBS, obtained from Gibco, USA) and 1% Approximately 2 × 10 in 96 well plates using DMEM (Dulbecco's Modified Eagle Medium, Lonza, USA) medium incorporating Penicillin-Streptomycin (Gibco, USA) After dispensing at a density of ⁴ cells / well, the cells were incubated for 24 hours at 37 ° C. and 5% CO 2. After the culture solution was removed, the cells were washed once with 200 ul of phosphate buffered saline (PBS), and 200 ul of DMEM medium containing fetal bovine serum was added and incubated for 24 hours. After treating Glu-Phe, Glu-Tyr and other dipeptide materials with 0.01% concentration for 10 minutes with DMEM medium containing 1% fetal calf serum, further treatment with 10 ppm sodium lauryl sulfate and incubated for 24 hours. I was. Interleukin-6 (IL-6) activity by sodium lauryl sulfate using Interleukin-6 (IL-6) ELISA kit (obtained by BD pharmigen, USA) using 50 ul of culture medium Inhibitory effect was measured and the results are shown in Table 3 below.

The inhibitory capacity was calculated based on the absorbance of Recombinant Interleukin-6, which is a standard material, to obtain a reaction formula between the absorbance and the standard material. The secretion amount of IL-6) was obtained. Then, the stimulation relaxation rate was evaluated by the following equation (2).

&Quot; (2) "

Stimulation alleviation rate (%) = 100 x [1- (Increased IL-6 secretion in the sodium lauryl sulfate and substance mixture group / IL-6 secretion in the sodium lauryl sulfate alone group)]

Test group Test concentration Stimulus Increased secretion amount (pg / ml) % Inhibition of stimulus IL-6 IL-6 Untreated control group 8.9 - Sodium lauryl sulfate 10 ppm 25.3 Hydrocortisone 5 uM Sodium Lauryl Sulfate 10 ppm 10.43 90.4% Glu-tyr 0.005% Sodium Lauryl Sulfate 10 ppm 13.9 69.1% Glu-ph 0.005% Sodium Lauryl Sulfate 10 ppm 12.9 75.4% Val-Lys 0.005% Sodium Lauryl Sulfate 10 ppm 55.9 -186.2%

The results of Table 3 show that even in the case of interleukin-6, Glu-Tyr and Glu-Phe components at 0.005% concentration were higher in interleukin- stimulation than sodium lauryl sulfate stimulation than when Val-Lys 0.005% was treated. 6 secretion inhibitory activity.

The soybean extract-containing composition according to the present invention is applicable to various formulations as follows, but is not limited thereto.

[Formulation Example 1] Soft capsule

100 mg of soybean extract of Example 1, soybean extract 50 mg, soybean oil 180 mg, red ginseng extract 50 mg, palm oil 2 mg, palm hardened oil 8 mg, lead 4 mg and lecithin 6 mg, 1 capsule according to a conventional method 400 mg of sugar was added to prepare a soft capsule.

[Formulation Example 2] Tablets

100 mg of soybean extract of Example 1, 50 mg of soy extract, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate and 40 mg of 30% ethanol were added to form granules, followed by 60 It was dried at 占 폚 and compressed into tablets using a tablet press.

[Formulation Example 3] Granules

100 mg of soybean extract of Example 1, 50 mg of soy extract, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch were mixed and 100 mg of 30% ethanol was added to form granules, followed by drying at 60 ° C. Formed and filled into fabric. The final weight of the contents was 1 g.

[Example 4] Drinks

100 mg of soybean extract of Example 1, 50 mg of soy extract, 10 g of glucose, 50 mg of red ginseng extract, 2 g of citric acid and 187.8 g of purified water were mixed and filled into bottles. The final dose of the contents was 200 ml.

[Formulation Example 5] Preparation of health food

Soybean extract of Example 1 ...................................... 1000 mg

Vitamin mixture

Vitamin A Acetate ......... 70 μg

Vitamin E ............................................ 1.0 mg

Vitamin B1 ........................................... 0.13 mg

Vitamin B2 .......................................... 0.15 mg

Vitamin B6 ......................................... 0.5 mg

Vitamin B12 ......................... 0.2 μg

Vitamin C ............................................. 10 mg

Biotin ... 10 ㎍

Nicotinic acid amide .................................. 1.7 mg

Folic acid ................................................. ..... 50 μg

Calcium pantothenate .................................... 0.5 mg

Mineral mixture

Ferrous Sulfate ......................................... 1.75 mg

Zinc oxide ............................................ 0.82 mg

Magnesium carbonate ...................................... 25.3 mg

Potassium Phosphate ......................................... 15 mg

Secondary calcium phosphate ...................................... 55 mg

Potassium citrate ............................................ 90 mg

Calcium carbonate .............................................. 100 mg

Magnesium chloride ..................................... 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

Preparation Example 6 Preparation of Healthy Drinks

Soybean Extract of Example 1 ...................................... 1000 mg

Citric acid ................................................. ... 1000 mg

oligosaccharide................................................. .... 100 g

Plum concentrate ................................................ ..... 2 g

Taurine ................................................. ........... 1 g

Purified water was added to the mixture to complete 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated at 85 DEG C for about 1 hour with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, ≪ / RTI >

[Formulation Example 1] Softening lotion (skin lotion)

Compounding ingredient Content (% by weight) Soybean Extract of Example 1 0.0025 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 2] Nutritional lotion (Milk lotion)

Compounding ingredient Content (% by weight) Soybean Extract of Example 1 0.0025 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capric triglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Liquid paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 3] Nourishing cream

Compounding ingredient Content (% by weight) Soybean Extract of Example 1 0.0025 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 4] Massage cream

Compounding ingredient Content (% by weight) Soybean Extract of Example 1 0.0025 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 5] Pack

Compounding ingredient Content (% by weight) Soybean Extract of Example 1 0.0025 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 Good Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 6] ointment

Compounding ingredient Content (% by weight) Soybean Extract of Example 1 0.0025 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservative, coloring, fragrance Suitable amount Purified water Balance

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (20)

Soybean extract or a fraction thereof as an active ingredient,
The soybean extract or fractions thereof contains an anti-inflammatory activity dipeptide 700 g / g or more based on the total weight of the extract or fraction, anti-inflammatory composition. The anti-inflammatory composition of claim 1, wherein the dipeptide is one or more of Glu-Phe and Glu-Tyr. The anti-inflammatory composition according to claim 1, wherein the extract or fraction has an anti-inflammatory activity with a stimulatory inhibitory rate of 40% or more compared to the untreated group. The anti-inflammatory composition of claim 1, wherein the soybean is fermented soybean. The anti-inflammatory composition of claim 1, wherein the extract or fraction is contained in an amount of 0.001 to 30% by weight based on the total weight of the composition. The method of claim 1, wherein the flowers are brown, chrysanthemum, corolla, Kwanhwa, chrysanthemum, gold and silver, flower, homegrown, flower, rose flower, wheat dream, windproof, balsam, pomegranate, sun flower, line drawing, pine, shin Anti-inflammatory composition, which is at least one selected from the group consisting of softening, Wonhwa, Takhwa, gunhwa, sulphate and safflower. The anti-inflammatory composition of claim 1, wherein the soybean is at least one selected from the group consisting of frosted, seomoktae, black, chungtae, yellow, hedgehog, kidney bean, ground kidney bean, red bean, soybean, bean sprout bean and soybean. The anti-inflammatory composition of claim 1, wherein the composition is for reducing interleukin secretion. The composition of claim 8, wherein the interleukin is interleukin-8 or interleukin-6. The anti-inflammatory composition according to any one of claims 1 to 9, wherein the composition is a pharmaceutical composition. 10. The anti-inflammatory composition according to any one of claims 1 to 9, wherein the composition is a health food composition. The anti-inflammatory composition according to any one of claims 1 to 9, wherein the composition is a cosmetic composition. As a method of producing soybean extract for anti-inflammatory, the method
(a) applying heat to the beans;
(b) fermenting the beans with flowers; And
(c) extracting the fermented soybeans. The method of claim 13, wherein step (b) is a step of fermenting the beans with a flower at 5-40 ° C for 3-10 days. The method of claim 14, wherein step (b) is a step of fermenting the beans with a flower at room temperature for 3 to 10 days. The method according to any one of claims 13 to 15, wherein the manufacturing method is after the step of fermentation,
(b) removing the flowers from the fermented soybeans. The method according to any one of claims 13 to 15, wherein the fermentation is placed in a container and left to stand. 18. The method of claim 17, wherein the container is an onggi. The flower of claim 13, wherein the flowers are brown, chrysanthemum, cauliflower, Kanto, lump, gold and silver, flower, homegrown, flower, flower, wheat, flower, balsam, pomegranate, sun flower, line flower, pine flower, fresh flower, One or more selected from the group consisting of softening, Wonhwa, Takhwa, totalization, sulphate and safflower, a method for producing an anti-inflammatory bean extract. The anti-inflammatory bean extract of claim 13, wherein the soybean is at least one selected from the group consisting of frosted, seomoktae, black, chungtae, yellow, hedge bean, kidney bean, ground kidney bean, red bean, soybean, bean sprout bean and soybean. Manufacturing method.