KR20130011800A - O/w emulsion having skin lipid and cosmetic composition comprising the same - Google Patents

Abstract

The present invention relates to an oil-in-water emulsifying composition comprising a skin-like lipid and a cosmetic composition comprising the same, and more particularly, to an intercalation particle by including an amphiphilic lipid complex composed of glycosyl cerebromide, phospholipid, and cholesterol. The present invention relates to an oil-in-water emulsifying composition capable of maintaining high dispersion stability by suppressing aggregation or coalescence and a cosmetic composition comprising the same.

Description

Oil-in-water emulsifying composition comprising skin-like lipids and cosmetic composition comprising same {O / W Emulsion having skin lipid and cosmetic composition comprising the same}

The present invention relates to an oil-in-water emulsified composition comprising a skin-like lipid that maintains high dispersion stability due to no aggregation or coalescence between particles and a cosmetic composition comprising the same.

Emulsion system composed of fine particles is an interface of nanoemulsion prepared by solubilizing an effective substance in nano units, liposomes prepared using self-association of phospholipids, lipid nanoparticles obtained by atomizing solid lipids, and surfactants. The research on the polymer nanoparticles which stabilized, etc. has been extensively performed.

But nanoparticles are colloidal instability due to their small size. That is, it belongs to a thermodynamically unstable system because free energy increases due to the interfacial increase between the continuous and dispersed phases. The particles of such a system increase in size due to the association between the particles, and precipitate, aggregate, and coalesce to affect the emulsified physical properties. In particular, as the oil phase content increases, such colloidal instability rapidly increases, and thus cannot contain a high content of oil phase.

Currently, various stabilizers, dispersants, and surfactants are used to increase colloidal stability of nanoemulsions. In particular, in the case of cosmetic compositions, lipids such as lecithin are most widely used to increase penetration into skin due to skin affinity.

Republic of Korea Patent Registration No. 10-0836035 relates to a nano emulsion having a particle size of 10 ~ 500nm, using a mixture of saturated and unsaturated lecithin as a surfactant, the unsaturated lecithin is 20 to 80% by weight relative to the total amount of the lecithin mixture It is proposed a nano emulsion prepared by the addition of one or more oils or bioactive active ingredients.

International Patent Publication No. 2008/010788 discloses plant sterols, cod liver oil, tocopherols, lecithin to produce improved nanoemulsions comprising ultra-small diameter nano-dimensional particles with diameters distributed over a uniform and clear range. , Lutein, zeaxanthin and soy protein.

Korean Patent Publication No. 2003-0085941 discloses dipalmitoylhydroxyproline and soy isoflavones in an amount of 0.01 to 10% by weight, 1 to 5% by weight of lecithin, 1 to 5% by weight of a solubilizer, and ka It is mentioned that liposomes containing 20 to 50% by weight of prilic / capric triglycerides, glycerol and alcohols can promote skin penetration and sustained release when used in cosmetic compositions.

In the case of the lecithin mentioned in the various patents, the liposome formulation produced by the lecithin can increase the stability in the process of solubilization, but it is not itself a medium for increasing the stability.

The increase in stability can be achieved by inducing an electrostatic repulsion on the outside of the particles with anionic and cationic surfactants and ionic polysaccharides, or by making modified liposomes conjugated with a polymeric material. Korean Patent Registration No. 10-0716802 refers to a polymer-liposomal nanocomposite containing a poly (methacrylic acid-co-n-alkyl methacrylate) copolymer and a lipid to be stably present in cosmetic formulations.

However, the emulsion formed to increase the stability can also increase the average size of the particles when stored for a long time, it is difficult to completely exclude the separation and destruction of the component in the case of the lipophilic active ingredient having an antioxidant effect.

In particular, in the case of oil-in-water emulsions, it is difficult to increase the ratio of the lipid to be solubilized, that is, the lipophilic component, and in order to increase the ratio of the lipophilic component, the addition of lecithin / surfactant or the addition of an additional stabilizer is required. There is a risk of skin toxicity when used. In addition, there is a problem that causes a specific odor and bitter taste when used in food, the formulation has a disadvantage in that it is difficult to apply because the viscosity increases the usability, and may cause a problem in stability.

Republic of Korea Patent Registration No. 10-0836035 International Patent Publication No. 2008/010788 Republic of Korea Patent Publication No. 2003-0085941 Republic of Korea Patent Registration No. 10-0716802

In order to solve the problems of the prior art, the present inventors have found an excellent stability of a creamy composition having lipid-particle particles on the order of several hundreds to hundreds of nm without using lecithin / surfactant. I wanted to develop.

Accordingly, an object of the present invention is to provide an oil-in-water emulsion composition using the amphiphilic lipid complex of the novel composition and a method for preparing the same.

In addition, the present invention has another object to provide a cosmetic composition comprising the oil-in-water emulsion composition.

In order to achieve the above object, the present invention provides an oil-in-water emulsified composition comprising an amphiphilic lipid complex consisting of glycosyl cerebroside, phospholipid, and cholesterol.

In addition,

S1) preparing an aqueous phase including an amphiphilic lipid complex, a humectant and purified water;

S2) preparing an oil phase including a high melting point lipid and a low melting point lipid on a liquid oil;

S3) adding a water phase to the oil phase to prepare a preemulsion;

S4) provides a method for preparing an oil-in-water emulsion composition to perform the ultra-high pressure emulsification process after cooling the preemulsion.

The present invention also provides a cosmetic composition comprising the oil-in-water emulsion composition.

Emulsifying composition according to the present invention can effectively encapsulate the liquid oil component in which the lipid is oil phase through the appropriate combination ratio of the liquid oil and the lipid component of the skin-like substrate to maintain a high dispersion stability without causing aggregation or coalescence between particles. . Accordingly, unlike general microcapsules, there is an advantage that the foreign body does not feel when applied on the skin by not using an insoluble component.

In particular, the emulsified composition, unlike the general emulsification system, by implementing a skin-friendly lipid emulsification system is excellent in skin safety and moisturizing sustainability and improves skin elasticity, skin texture, and skin thickness due to skin-like substrate as well as skin density Since it can improve the high value as a cosmetic can be secured.

1 is a photograph measured using a cold field emission scanning microscope (Cryo-FE-SEM) to confirm the particles of the cream emulsion composition prepared in Example 1.
FIG. 2 is a Turbiscan graph showing dispersion stability as a result of measuring the cream emulsion composition obtained in Example 1, Comparative Examples 1, 3, 4, and 6 at 60 ° C. for 3 days, respectively.
3 is a Turbiscan graph showing the dispersion stability as a result of measuring the cream emulsion composition obtained in Example 1, Comparative Examples 1, 3, 4 and 6, respectively, stored at room temperature (30 ℃) for 3 days.
4 is a graph showing the "Mean value kinetics" for the BS (%) change obtained through the Turbiscan test after storing the cream emulsion compositions of Examples 1, Comparative Examples 1, 3, 4, and 6 at 60 ° C.
FIG. 5 is a graph showing “Mean value kinetics” for the BS (%) change obtained through Turbiscan test after storage of the cream emulsion compositions of Examples 1, Comparative Examples 1, 3, 4, and 6 at 30 ° C. FIG.
Figure 6 (a) is a photograph showing the roughness of the skin before the use of the cream emulsion composition of Example 1, and (b) after 8 weeks.
Figure 7 (a) is a photograph showing the dermal density of the skin before and after the use of the cream emulsion composition of Example 1, (b) 8 weeks.

Hereinafter, the present invention will be described in more detail.

The present invention provides an aqueous type of creamy emulsion composition having nano-sized lipid capsule particles through a skin-like substrate.

Conventionally, lecithin is used as a lipid, and such lecithin is deteriorated in long-term storage, so that the association between particles in the preparation of nanoemulsion is inevitable. Accordingly, the present invention implements a skin-friendly lipid emulsification system using amphiphilic lipid complexes, not lecithin, as a lipid having a skin-like matrix, and effectively encapsulates liquid oil components and improves emulsion stability through high pressure emulsification technology. .

In the present invention, three kinds of lipids were selected as amphiphilic lipid complexes to prepare nanoemulsions having nano-sized lipid capsule particles. These amphiphilic lipid complexes are Glycosyl-cerebrosides, phospholipids, and cholesterol, which can be used in certain weight ratios to produce lipid capsules with a number average size of less than 300 nm.

Specifically, the total amount of the amphiphilic lipid complex is mixed with 65 to 98.9% by weight of glycosyl cerebromide, 0.1 to 30% by weight of phospholipid, and 1.0 to 10.0% by weight of cholesterol.

Amphiphilic lipid complexes having the above combinations can enhance the dispersion stability of the nanoemulsion for a long time during emulsion preparation by the high pressure emulsification method, and due to the structural characteristics of the nano-level lipid capsule particles more easily absorbed and penetrated the skin barrier It helps to double the function of the intended cosmetics.

Lecithin is an amphoteric ionic emulsifier and consists of three kinds of phospholipids: phosphatidylcholine (PC; phosphatidylcholine); Phosphatidylethanolamine (PE; Phosphatidylethanolamine) and phosphatidyl inositol (PI; Phosphotidylinositol), a combination of phospholipids. In contrast, the amphipathic lipid complex used in the present invention is composed of glycosyl cerebromide, phospholipid, and cholesterol, and glycosyl cerebromide is generally a form in which glucose or galactose is bound by glycosidic bond to ceramide. Unlike common neutral ceramides, which are sparingly soluble, are water-soluble lipids soluble in water phases. Thus, amphiphilic lipid complexes can enhance emulsion stabilization by tightening the lipid capsule particle membrane with glycosyl cerebroside and cholesterol in the self-associative structure of phospholipid.

In the amphiphilic lipid complex, glycosyl cerebroside is contained in excess of other phospholipids or cholesterol, and when used below the above range, lipid capsule particle size is increased to 300 nm or more, and particles aggregate together during long-term storage. Occurs and dispersion stability is lowered. On the contrary, when used in excess of the above range, the content of the relatively different composition is reduced, which also leads to an increase in the size of the lipid capsule particle, so that it is appropriately used in the range of the indicated content.

These glycosyl cerebrosides, phospholipids, and cholesterol can be used by purchasing and mixing commercially available products or commercially available products made of these.

The amphipathic lipid complexes described above can be used to prepare nanoemulsions having nano-level lipid capsule particles. In particular, the present invention prepares an oil-in-water emulsion composition using the liquid oil, the high melting point lipid and the low melting point lipid as the oil phase together with the amphiphilic lipid complex.

Preferably, the ratio of each composition constituting the oil-in-water emulsified composition is to satisfy the total 100% by weight.

a) i) 0.1-5.0 wt.% amphiphilic lipid complex,

 Ii) 5.0-30.0% by weight of a moisturizer,

Iii) 0.01∼1.0 wt% of water thickener,

Iii) an aquatic part containing water as remainder,

b) i) high melting point lipid 2.0-10.0 weight%,

Ii) 0.5-10.0% by weight of low melting lipids, and

Iii) It consists of oil phase part containing 2.0 ~ 25.0% by weight of liquid oil.

The amphiphilic lipid complex is used in an amount of 0.1 to 5.0% by weight, preferably 0.5 to 2.0% by weight in the total composition. If the content of the amphiphilic lipid complex is less than the above range, the formulation of capsules may not be sufficiently encapsulated, resulting in poor formulation stability. On the contrary, if the content of the amphiphilic lipid complex exceeds the above range, a high hardness cream formulation may be produced due to excessive use of the lipid component. It is not easy and it is difficult to take advantage of the appearance and usability of the present invention.

Usable humectants include polyhydric alcohols such as glycerin, erythritol, xylitol, maltitol glycerin, propylene glycol, 1,3-butylene glycol, sorbitol, polyglycerin, polyethylene glycol, 1,2-pentanediol and isoprene glycol; Ingredients such as amino acids, sodium lactate, sodium pyrrolidone carboxylate, xyloglucan, quince seed, carrageenan, pectin, met, cranlan, galactan, delmantan sulfate, glycoken, keratan sulfate, chondroitin, mucoy Tin Sulfate, Keratosulfate, Chondroitin, Mucoitin Sulfate, Keratosulfate, Locust Bean Gum, Succinoglucan, Caronym Acid, Hyaluronic Acid, Heparan Sulfate, Sodium Hyaluronate, Collagen, Mucopolysaccharide, Chondroitin Water-soluble high molecular materials which are sulfuric acid; Dimethyl polysiloxane, methylphenyl siloxane; Culture supernatant of lactic acid bacteria and bifidobacteria; And a combination of these.

The moisturizer is used in an amount of 5.0 to 30.0 wt%, preferably 5.0 to 20.0 wt%, in the total oil-in-water emulsion composition. If the content is less than the above range, a sufficient moisturizing effect cannot be imparted. On the contrary, if the content exceeds the above range, the content of the other composition is relatively reduced, so that the effect thereof cannot be secured.

The water-based thickener is to increase the feeling of use by imparting an appropriate viscosity when used as a cosmetic composition, using one selected from the group consisting of carbomer, gelatin, xanthan gum, natural cellulose, high cell, methyl cellulose and combinations thereof. Preferably carbomers are used which are able to transparently increase over a wide range of pH, and at low concentrations.

The water thickener is used in an amount of 0.01 to 1.0% by weight, preferably 0.1 to 0.5% by weight in the total oil-in-water emulsion composition. If the content is less than the above range, the cream may not have a hardness enough to inhibit creaming or sedimentation, whereas if the content exceeds the above range, the cream is too hard to be applied as a cosmetic and the skin is difficult to apply. Since the foreign material feeling by the thickener is felt at the time of application, use suitably within the said range.

Water is distilled water, preferably deionized distilled water is used.

The high melting point lipid according to the present invention uses at least three lipids selected from the group consisting of batyl alcohol, behenyl alcohol, cetostearium alcohol, cetyl alcohol, stearyl alcohol. The high melting point lipid may harden the emulsion film of the nanoemulsion due to the high melting point, thereby preventing particles from agglomerating or coalescing at a high temperature, thereby increasing stability of the cream emulsion composition. As a result of using two types of high melting point lipids in Comparative Examples 4 to 6 of the present invention, the stability over time was low, and aggregation occurred during storage at room temperature and high temperature. Preferably, as the high melting point lipid, batyl alcohol, behenyl alcohol, cetostearic alcohol are used, and these are 1.0: 0.5: 0.5 to 1.0: 1.5: 2.0 (weight ratio), more preferably 1.5: 0.7: 1.3 Use by weight ratio.

The high melting point lipid is used in an amount of 2.0 to 10.0% by weight, preferably 3.0 to 5.0% by weight in the total oil-in-water emulsion composition.

If the content is less than the above range, the stability at room temperature and high temperature cannot be sufficiently secured. On the contrary, if the content exceeds the above range, it is not easy to prepare the emulsion by forming high hardness, so that it is suitably used within the above range.

Low melting point lipids improve the stability of emulsification through the buffering role in rapid temperature changes, and play a role of smoothly applying to the skin without feeling foreign body. The low melting point lipids that can be used include one selected from the group consisting of mango butter, shea butter, copoa seed seed butter, macadamia seed oil, and combinations thereof, and preferably mango butter and macadamia seed oil are used.

The low melting point lipid is used in an amount of 0.5 to 10.0% by weight, preferably 1.0 to 5.0% by weight in the total oil-in-water emulsion composition. If the content is less than the above range, there is no expected effect as described above. On the contrary, if the content exceeds the above range, it is burdensome and heavy to use on the skin, and thus it is appropriately used within the above range.

In addition, the liquid oil used in the present invention may be an oil commonly used in this field so as to dissolve high melting point and low melting point lipids sufficiently. Typically, hydrocarbon-based oils such as polydecene and paraffin oil; Ester synthetic oils; Silicone oil; Animal and vegetable oils; Ethoxylated alkyl ester oils; cholesterol; Cholesteryl sulfate; Phytosphingosine; Sphingoidoid lipids; One selected from the group consisting of C10-40 fatty alcohols, caprylic / capric triglycerides, ceramides and mixtures thereof may be used. Preferably, pentaerythritol tetraethylhexanoate, caprylic / capric triglycerides, vegetable squalane, and hydrogenated C 6-14 olefin polymers are used. The liquid oil is used in an amount of 2.0 to 25.0% by weight, preferably 5.0 to 20.0% by weight in the total oil-in-water emulsion composition. If the content is less than the above range, the oil content is so small that it is difficult to take advantage of the cream formulation, which is different from the general solubilizing gel cream formulation, whereas if the content exceeds the above range, it is difficult for the lipid components to effectively encapsulate the oil particles. Since the long-term stability of deteriorates rapidly, it is suitably used within the above range.

In addition to the above-described composition may be used a variety of compositions, for example hydrocarbon; And one fatty acid, silicone polymer stabilizer selected from the group consisting of lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, and mixtures thereof. Examples of the silicone polymer include bispyg 15 / methylethyldimethyl silane, dimethicone / dimethicone Fiji-10 / 15, dimethicone / polyglycerin-3, dimethicone / dimethiconol, dimethicone / dimethicone vinyl dimethicone , Cyclomethicone / dimethiconol, cyclomethicone / dimethicone, cyclomethicone / trimethylsiloxysilicate, cyclopentasilonic acid / dimethicone, cyclopentasilonic acid / fiji-12 dimethicone, cyclopentasilonic acid / It is preferred that at least one selected from the group consisting of cetearyl dimethicone / vinyl dimethicone, cyclopentasilonic acid / dimethicone / vinyl dimethicone, dimethicone / vinyl dimethicone crosspolymer.

In addition, if necessary, it may further include a solvent or additive as known in the art. The solvent is used to dissolve the oil-soluble component, methanol, ethanol, propanol, butanol and mixed solvents thereof may be used. In addition, the additives may be preservatives, pH adjusters, surfactants, antioxidants, sunscreens, pigments, dyes, fragrances and the like, and may be appropriately selected by those skilled in the art.

The method for preparing the oil-in-water emulsion composition according to the present invention is not particularly limited, and an emulsification method that is commonly used, preferably a high pressure emulsification method, may be used. The high pressure emulsification method uses a microfluidizer (MF), and has an advantage of preparing a stable nanoemulsion while using a minimum amount of an emulsifier. Therefore, it is widely used in research to develop new products that are stable to skin and can control skin absorption.

Specifically, the oil-in-water emulsion composition

S1) preparing an aqueous phase including an amphiphilic lipid complex, a humectant, and purified water;

S2) preparing an oil phase including a high melting point lipid and a low melting point lipid on a liquid oil;

S3) adding a water phase to the oil phase to prepare a preemulsion;

S4) performing a high pressure emulsification process after cooling the preemulsion;

S5) Prepared through the step of degassing after mixing the aqueous phase thickener and other additives to the obtained composition.

Each step will be described in more detail below.

First, an aqueous phase including an amphiphilic lipid complex, a humectant, and purified water is prepared (S1).

At this time, the dissolution is carried out in a vacuum emulsification tank capable of temperature control and stirring by a conventional method, it is carried out so as to be sufficiently dissolved in other water by stirring or heating to 70 ~ 85 ℃ as needed.

Next, an oil phase portion including a high melting point lipid and a low melting point lipid is prepared on the liquid oil (S2).

At this time, the production of the oil phase is also carried out so as to be sufficiently dissolved in the oil by stirring or heating to 70 ~ 85 ℃ as needed, such as the water phase.

Next, the water phase portion is added to the oil phase to prepare a preemulsion (S3).

The preparation of the pre-emulsion is to form a bulky emulsion through primary emulsification by performing stirring for 3 to 10 minutes at a speed of 2000 to 4000 rpm, preferably 3000 rpm using a homo mixer in a vacuum emulsification tank. Is done.

Next, the pre-emulsion is homogenized in an ultrahigh pressure emulsifier after cooling to prepare an emulsion composition (S4).

The cooling is performed by lowering the prepared preemulsion to 50 ~ 60 ℃ to adjust the temperature suitable for emulsification.

The cooled pre-emulsion is put into a high-pressure homogenizer or high-pressure emulsifier commercialized at a temperature of 50 ~ 60 ℃, and treated once at a pressure of 500 ~ 1500bar to prepare a nanoemulsion. At this time, if the temperature or the processing pressure is less than the above range, it is difficult to stably form a sufficient pre-emulsion nanoscale, on the contrary, if it exceeds the above range, it may cause damage to the physiologically active ingredient itself due to a strong force or temperature or rather to aggregate particles. Cannot produce sufficiently small emulsions of nano size.

Next, the oil-in-water emulsified composition is prepared through a step of degassing after mixing the aqueous phase thickener and other additives to the obtained emulsion composition (S5).

The oil-in-water emulsion composition thus prepared is obtained by using an amphiphilic lipid complex in combination with high melting point and low melting point lipids and liquid oils to obtain nanoemulsions with an average size of lipid capsule particles of 300 nm or less, and without using chemical surfactants. High stability can be secured at room temperature. In particular, it is a skin-friendly lipid emulsifying system using amphiphilic lipid complexes, which provides excellent skin safety and moisturizing sustainability, and gives new value as a cosmetic by providing effects of skin elasticity, skin texture, and skin thickness due to skin-like substrates. Do.

When used as a cosmetic composition, the content of the oil-in-water emulsified composition is used in an amount of 0.001 to 99.9% by weight, based on the total weight of the total cosmetic composition, the content is appropriately adjusted according to the formulation of the cosmetic composition.

The cosmetic composition of the present invention may be prepared in any formulation as is known and may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, emulsion foundation, wax foundation, and the like.

In one example, it may be used as a basic cosmetic composition selected from softening cosmetics, nourishing cosmetics, lotions, creams, packs, gels, or patches, a color cosmetic composition selected from lipsticks, makeup bases, or foundations.

In addition, the compositions of each formulation may contain various bases and additives necessary and appropriate for the formulation of the formulation, and nonionic surfactants, silicone polymers, extender pigments, fragrances, preservatives, Fungicides, oxidation stabilizers, organic solvents, ionic or nonionic thickeners, softeners, antioxidants, free radical destroyers, opacifiers, stabilizers, emollients, silicones, α-hydroxy acids, antifoams, humectants, It is prepared by including known compounds such as vitamins, insect repellents, perfumes, preservatives, surfactants, anti-inflammatory agents, fillers, polymers, propellants, basicizing or acidifying agents, or coloring agents.

[Example]

Hereinafter, the present invention will be described in more detail with reference to examples. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. Embodiments of the present invention are provided to more fully explain the present invention to those skilled in the art.

Example  And Comparative example : Preparation of Cream Emulsifying Composition

After dissolving the water phase part and the oil phase part to 80 ° C. to completely dissolve the oil phase part, the oil phase part was slowly added to the water phase part and emulsified at 3000 rpm using a homo mixer for 5 minutes to prepare a pre-emulsion. The preemulsion was cooled to 45 ° C. and then passed once under a pressure of 1300 bar using a microfluidizer. After the addition of the aqueous thickener and additives, and stirred for 3 minutes at 3000rpm with a homogenizer, and then degassed and cooled to 30 ℃ to prepare an oil-in-water cream emulsion composition. At this time, the composition of the water phase part and the oil phase part is shown in Tables 1 to 3 below. At this time, the amphipathic lipid complex used was glycosyl cerebromide, phospholipid, and cholesterol of 70: 25: 5% by weight.

Composition (% by weight) Example 1 Example 2 Example 3 4 to implementation Example 5 Awards
part Amphiphilic lipid
Complex A 1.0 1.0 1.0 1.0 1.0 glycerin 6.0 6.0 6.0 6.0 6.0 Butylene Glycol 7.0 7.0 7.0 7.0 7.0 Bis-Pig-18-Methylethyldimethylsilane 2.0 2.0 2.0 2.0 2.0 Xanthan gum 0.1 0.1 0.1 0.1 0.1 Paid
part Batyl alcohol 1.5 1.5 1.5 1.5 1.5 Behenyl Alcohol 0.7 0.7 0.7 0.7 0.7 Cetostearyl alcohol 1.3 1.3 1.3 1.3 1.3 Mango Butter 1.0 1.0 - - 1.0 Sheer butter - - 1.0 - - Copoasu Seed Butter - - - 1.0 - Macadamia Seed Oil 2.0 2.0 2.0 2.0 2.0 Pentaerythritol tetraethylhexanoate 3.0 - - - - Caprylic / Capric Triglycerides 3.0 6.0 6.0 6.0 3.0 Vegetable squalane 3.0 8.0 8.0 8.0 4.0 Hydrogenated C 6-14 Olefin Poly 5.0 - - - - silicon Dimethicone 0.5 0.5 0.5 0.5 0.5 Cyclomethicone 3.0 3.0 3.0 3.0 3.0 Thickener Carbomer 0.1 0.1 0.1 0.1 0.1 Metal Ion Blocker Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount pH regulator Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount antiseptic Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Purified water Balance Balance Balance Balance Balance

Composition (% by weight) Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Water top Amphiphilic lipid
Complex A - 6.0 - 1.0 1.0 1.0 1.0 Hydrogenated lecithin - - 1.0 - - - - glycerin 6.0 6.0 6.0 6.0 6.0 6.0 6.0 Butylene Glycol 7.0 7.0 7.0 7.0 7.0 7.0 7.0 Bis-Pig-18-Methylethyldimethylsilane 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Xanthan gum 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Floating Batyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Behenyl Alcohol 0.7 0.7 0.7 2.0 - 1.0 0.7 Cetostearyl alcohol 1.3 1.3 1.3 - 2.0 1.0 1.3 Mango Butter 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Macadamia Seed Oil 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Pentaerythritol tetraethylhexanoate 3.0 3.0 3.0 3.0 3.0 3.0 - Caprylic / Capric Triglycerides 3.0 3.0 3.0 3.0 3.0 3.0 12.0 Vegetable squalane 3.0 3.0 3.0 3.0 3.0 3.0 16.0 Hydrogenated C 6-14 Olefin Polymer 5.0 5.0 5.0 5.0 5.0 5.0 - silicon Dimethicone 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Thickener Carbomer 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Metal Ion Blocker Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount pH regulator Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount antiseptic Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Purified water Balance Balance Balance Balance Balance Balance Balance

Composition (% by weight) Comparative Example 8 Comparative Example 9 Comparative Example
10 Comparative Example
11 Comparative Example 12 Comparative Example 13 Comparative Example 14 Geology Amphipathic
Lipid Complex A 1.0 - - - - - - Amphipathic
Lipid Complex B - 1.0 - - - - - Hydrogenated lecithin - - - - - - - Glycosyl Cerebroside - - 1.0 - - 0.5 - Phospholipid - - - 1.0 - 0.5 0.5 cholesterol - - - - 1.0 - 0.5 Awards
part glycerin 6.0 6.0 6.0 6.0 6.0 6.0 6.0 Butylene Glycol 7.0 7.0 7.0 7.0 7.0 7.0 7.0 Bis-Pig-18-Methyletherdimethyl Silane 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Xanthan gum 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Paid
part Batyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Behenyl Alcohol 0.7 0.7 0.7 0.7 0.7 0.7 0.7 Cetostearyl alcohol 1.3 1.3 1.3 1.3 1.3 1.3 1.3 Mango Butter 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Macadamia Seed Oil 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Pentaerythritol tetraethylhexanoate - 3.0  3.0 3.0 3.0 3.0 3.0 Caprylic / Capric Triglycerides 15.0 3.0  3.0 3.0 3.0 3.0 3.0 Vegetable squalane 20.0 3.0  3.0 3.0 3.0 3.0 3.0 Hydrogenated C 6-14 Olefin Polymer - 5.0 5.0 5.0 5.0 5.0 5.0 silicon Dimethicone 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Thickener Carbomer 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Metal Ion Blocker Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount pH regulator Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount antiseptic Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Purified water Balance Balance Balance Balance Balance Balance Balance Amphiphilic Lipid Complex A: = Glycosyl Cerebroside: Phospholipid: Cholesterol = 70: 25: 5 (wt%)
Amphiphilic Lipid Complex B: = Glycosyl Cerebroside: Phospholipid: Cholesterol = 50: 25:25 (wt%)

< Test Example  1> particle size

In order to confirm the lipid capsule particles of the cream emulsion composition prepared in Example 1 was measured using a cold field emission scanning microscope (Cryo-FE-SEM, HITACHI S-4700), the results are shown in FIG.

Referring to Figure 1, the diameter of the lipid capsule particles is measured to be 278nm, it can be seen that the cream emulsified composition has a nano-level lipid capsule particles.

< Test Example 2> Stability Evaluation

The cream emulsion compositions prepared in Examples and Comparative Examples were stored at room temperature, 45 ° C. and 40 ° C. for one month in a circulating (Cycling) thermostatic chamber, and the stability of the cream emulsion composition was observed according to the occurrence of hardness drop, oil flotation and separation. The results are shown in Table 4 below.

Cream stability over temperature and time 45 ° C, 1 month stability 40 ° C, 1 month stability (-15 ～ -40 ℃) Stability over 1 month
cycle Example 1 Good Good Good Example 2 Good Good Good Example 3 Good Good Good Example 4 Good Good Good Example 5 Good Good Good Comparative Example 1 Oil separation Oil separation Oil separation Comparative Example 2 Good Good Gelation Comparative Example 3 Oil floating Oil floating Oil floating Comparative Example 4 Oil floating Good Good Comparative Example 5 Oil floating Good Good Comparative Example 6 Good Good Good Comparative Example 7 Oil floating Good Good Comparative Example 8 Oil floating Good Oil floating Comparative Example 9 Good Good Gelation Comparative Example 10 Oil Good Gelation Comparative Example 11 Oil Good Good Comparative Example 12 Oil Good Oil Comparative Example 13 Oil Good Oil Comparative Example 14 Oil Good Oil

Referring to Table 4, the cream emulsified composition of Example 1 according to the present invention was observed for 1 month at high temperature (45 ℃, 40 ℃, Cycling) conditions, the stability was very excellent, compared to Comparative Examples 1 to 1 The cream emulsion composition of 14 was found to be inferior in stability.

In particular, the cream emulsified composition of Comparative Example 6 was good for each condition, but it was confirmed that the stability of the particles was lowered at higher temperature stability than the cream emulsified composition of Example 1 over time.

< Test Example  3> Stability Assessment ( Turbiscan  analysis)

Turbiscan can qualitatively and quantitatively measure the change in dispersion stability over time under certain temperature conditions, taking into account the dispersion of various samples in terms of concentration and particle size.

The cream emulsion compositions prepared in Examples and Comparative Examples were scanned every 1 hour at 60 ° C. and 30 ° C. for 3 days to measure changes in emulsion stability (ie, dispersion stability) for a total of 3 days. 3 is shown.

2 and 3 store the cream emulsion composition obtained in Example 1 (a), Comparative Examples 1, 3, 4 and 6 (b, c, d, e) for 3 days at 60 ℃ and room temperature (30 ℃), respectively Turbiscan graph showing dispersion stability as measured.

In this experiment, Tubiscan AGS (Formulaction, France) was used, through which the flux of transmitted and backscattered light (Flux;%) was varied depending on the size of each particle in the emulsion composition and the volume fraction of the dispersed and continuous phases. ) Were measured simultaneously. In this case, it is a graph showing the backscattering profile (or transmission) change (Y axis) according to each scan time with respect to the height (X axis) of the sample in the reference mode. The results are given in the reference mode to the 2 ^nd profile as the reference time. In addition, in order to make visual comparison of each sample easy, the x-axis was made to the Meniscus of each sample, and the y-axis was shown to the same scale as +32--20%.

In this case, since the stability change due to aggregation occurs in the entire cream emulsified composition, it can be confirmed by changing the backscattering flux (BS (%)) in the middle region of the Turbiscan graph, where BS (% ) Decrease levels indicate that aggregation occurs. In addition, changes in the bottom and top regions can confirm whether or not phase separation has occurred due to sedimentation / creaming, and an increase in the upper BS (%) value results in a cream phenomenon and a lower BS (%). Decreasing the value means that precipitation occurs.

2 and 3, it can be seen that the cream emulsion composition obtained according to the present invention stably maintains the dispersed phase at a high temperature (60 ℃) and room temperature (30 ℃). On the other hand, in the case of the cream emulsion composition of Comparative Examples 1 to 6, aggregation was common, and in some, sedimentation / creation by particle migration was observed.

4 and 5 show the "Mean value kinetics" for the BS (%) change obtained through the Turbiscan test after the cream emulsion compositions of Examples 1, Comparative Examples 1, 3, 4, and 6 were stored at 60 ° C and 30 ° C. "Is a graph.

4 and 5, the cream emulsion composition of Example 1 showed a high dispersion stability, the cream emulsion composition of Comparative Examples 1, 3, 4 and 6 it can be seen that the change width is large, the dispersion stability is lowered have. These results can be clearly seen from the results of the Turbiscan stabilization index of Table 5 below.

Example 1 Comparative Example 1 Comparative Example 3 Comparative Example 4 Comparative Example 6 Stability Index 0.23 0.99 1.46 0.51 0.42

< Test Example  4> Human Skin Info Test

In order to confirm the stability of the composition of the above prepared to the skin was determined whether the skin irritation, the results obtained are shown in Table 6.

The test was symmetrically closed on the inner region of the upper arm, the scapula under the scapula, and 20 healthy adult male and female experimenters. At this time, after 24 hours after attaching the patch treated with each composition to the test site, the patch was removed and the position was indicated. After 1 hour, the reaction was determined and recorded. After 24 hours and 48 hours, the reaction was determined and recorded.

<Judge criteria>

No reaction at all:-: negative judgment

Faint erythema: +: false positive

Erythema: ++: positive

Edema, papules: +++: neutral

Minor vesicles, necrosis: ++++: kangyangseong

Condition Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Judgment - - - - - - - Condition Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Judgment - - - - - + - Condition Comparative Example 10 Comparative Example 11 Comparative Example 12 Comparative Example 13 Comparative Example 14 Judgment - - - - -

Referring to Table 6, in the case of the emulsion composition of Example 1 according to the present invention it was shown that there is no skin irritation.

In addition, subjective questionnaire evaluation and safety evaluation (skin adverse reaction evaluation by a dermatologist and a subject) of the cream composition of Example 1 according to the present invention were performed. As a result, the cream of Example 1 used in the human application test did not show any symptoms such as itching or human symptoms such as special skin abnormality (vesicle) during the sample evaluation period.

< Test Example  5> skin application test

In order to confirm the effect of improving the skin thickness, dermal density, and skin texture when the cream having the oil-in-water emulsion composition according to the present invention is applied to the skin, a human application test was performed as follows.

-Institution: Ellead

Subjects: 21

-How to use the sample: Wash every morning / evening (twice a day) and then toner → eye cream → essence → eye cream → essence → emulsion → cream (cream of Example 1)

-Assessment Methods:

Instrumental Evaluation (Cutometer, Dermascan-C, PRIMOS High Resolution)

Subjective Survey Evaluation by Subject

Safety assessment (evaluation of skin adverse events by dermatologists and subjects)

Mechanical  evaluation

Using the cream having an oil-in-water emulsifying composition according to the present invention, skin elasticity, skin pore volume, dermal density, and skin texture were measured and the results obtained are shown below.

(1) Skin elasticity : skin elasticity was measured using a cutometer, and the measured elasticity (R2) obtained at this time was analyzed to show skin elasticity improvement rates shown in Tables 7 to 9 below.

Skin elasticity measurement result (R2) Before use 2 weeks after use 4 weeks after use 8 weeks after use Average 0.58 0.68 0.74 0.77 Standard Deviation 0.08 0.05 0.08 0.09

Skin elasticity improvement rate (%) 2 weeks after use 8 weeks after use 8 weeks after use Improvement rate 16.15 27.64 31.98

Skin elasticity statistical analysis result 2 weeks after use 8 weeks after use 8 weeks after use p value 0.000 0.000 0.000

P value: p <0.05

Looking at the Table 7 to Table 9, compared with before using the cream emulsion composition of Example 1 shows a skin elasticity improvement rate of 16.15% after 2 weeks, 27.64% after 4 weeks, 31.98% after 8 weeks, the composition according to the present invention It can help to improve skin elasticity.

(2) Skin thickness : Skin thickness was measured using Dermascan-C, and the obtained skin thickness improvement rate was analyzed by analyzing the measured value (mm) obtained in Table 10 to Table 12.

Skin thickness measurement result (mm) Before use 2 weeks after use 4 weeks after use 8 weeks after use Average 1.48 1.62 1.69 1.74 Standard Deviation 0.18 0.12 0.11 0.13

Skin thickness improvement rate (%) 2 weeks after use 8 weeks after use 8 weeks after use Improvement rate 9.52 13.94 17.69

Skin thickness statistical analysis result 2 weeks after use 8 weeks after use 8 weeks after use p value 0.000 0.000 0.000

P value: p <0.05

Tables 10 to 12 show a skin thickness improvement rate of 17.69% after two weeks of use, four weeks of use, and eight weeks of use, compared to before the use of the cream emulsion composition of Example 1, wherein the skin composition according to the present invention It can help to improve skin thickness.

(3) Skin texture : The skin texture was measured using PRIMOS High Resolution, and the measured value (Ra) obtained at this time was analyzed and the skin texture improvement rates are shown in Tables 13 to 15 below.

Skin texture measurement result (Ra) Before use 2 weeks after use 4 weeks after use 8 weeks after use Average 16.83 15.29 15.46 14.55 Standard Deviation 3.00 2.70 1.67 1.79

Skin texture improvement rate (%) 2 weeks after use 8 weeks after use 8 weeks after use Improvement rate 9.14 8.17 13.56

Skin texture statistical analysis result 2 weeks after use 8 weeks after use 8 weeks after use p value 0.024 0.030 0.002

P value: p <0.05

Looking at the above Table 13 to Table 15, compared to before using the cream emulsion composition of Example 1 showed a skin texture improvement rate of 9.14% after two weeks, 8.17% after four weeks, 13.56% after eight weeks of use. In particular, there were statistically significant differences after 2 weeks, after 4 weeks of use, and after 8 weeks of use compared to before use. Therefore, the cream emulsified composition of Example 1 is believed to help improve skin texture after 2 weeks, 4 weeks, and 8 weeks after use compared to before use.

(4) Dermal Density : The dermal density was measured using Dermascan-C, and the measured values (AU) obtained at this time are shown in Tables 16 to 18 below.

Dermal Density Measurement Results (A. U.) Before use 2 weeks after use 4 weeks after use 8 weeks after use Average 7.57 7.81 7.86 7.95 Standard Deviation 0.60 0.40 0.36 0.22

Dermal Dense Density Improvement (%) 2 weeks after use 8 weeks after use 8 weeks after use Improvement rate 3.14 3.77 5.03

Dermal Density Statistical Analysis Results 2 weeks after use 8 weeks after use 8 weeks after use p value 0.096 0.030 0.008

P value: p <0.05

Looking at the Table 16 to Table 18, compared with before the cream emulsified composition of Example 1 showed 3.14% after two weeks of use, 3.77% after four weeks, 5.03% dermal density improvement after 8 weeks. In particular, after 4 weeks and 8 weeks after the use compared to the statistically significant difference was shown, it can be seen from the results that the skin composition according to the present invention helps to improve the dermal density.

These results can be clearly seen in the concrete photograph.

6 (a) is a photograph showing the roughness of the skin before and after the use of the cream emulsified composition of Example 1 (b) after 8 weeks, it can be seen that the skin roughness is significantly improved and smoothed after 8 weeks.

In addition, Figure 7 (a) is a photograph showing the dermal density of the skin before and after the use of the cream emulsifying composition of Example 1, (b) 8 weeks, it is shown that the dermal density of the skin significantly improved after using the cream emulsion composition Able to know.

Oil-in-water emulsion composition according to the invention can be applied to a variety of cosmetics.

Claims (15)

An oil-in-water emulsified composition comprising an amphipathic lipid complex consisting of glycosyl cerebroside, phospholipid, and cholesterol. The method of claim 1, wherein the amphiphilic lipid complex is 100% by weight in total,
65-98.9% by weight of glycosyl cerebroside,
0.1-30 wt% of phospholipid, and
An oil-in-water emulsified composition comprising 1.0 to 10.0% by weight of cholesterol. The oil-in-water emulsified composition according to claim 1, wherein the oil-in-water emulsified composition comprises 0.1 to 5.0% by weight of the amphiphilic lipid complex in its total composition. According to claim 1, wherein the oil-in-water emulsion composition to satisfy a total 100% by weight,
a) i) 0.1-5.0 wt.% amphiphilic lipid complex,
Ii) 5.0-30.0% by weight of a moisturizer,
Iii) 0.01∼1.0 wt% of water thickener,
Iii) an aquatic part containing water as remainder,
b) i) high melting point lipid 2.0-10.0 weight%,
Ii) 0.5-10.0% by weight of low melting lipids, and
I) An oil-in-water emulsified composition comprising an oil phase portion containing from 2.0 to 25.0% by weight of liquid oil. The method of claim 4, wherein the moisturizing agent is glycerin, erythritol, xylitol, maltitol glycerin, propylene glycol, 1,3-butylene glycol, sorbitol, polyglycerol, polyethylene glycol, 1,2-pentanediol, isoprene glycol Alcohols; Amino acids, sodium lactate, sodium pyrrolidone carboxylate, xyloglucan, quince seed, carrageenan, pectin, met, cardan, galactan, delmantansulfate, glycogen, keratan sulfate, chondroitin, mucoitin sulfate, Kerato sulfate, chondroitin, mucoitin sulfate, keratose sulfate, locust bean gum, succinoglucan, calonym acid, hyaluronic acid, heparan sulfate, sodium hyaluronate, collagen, mucopolysaccharide, chondroitin sulfate Polymeric materials; Dimethyl polysiloxane, methylphenyl siloxane; Culture supernatants which are lactic acid bacteria and bifidus bacteria; And oil-in-water emulsion composition characterized in that it comprises one selected from the group consisting of. The oil-in-water type according to claim 4, wherein the water thickener comprises one selected from the group consisting of carbomer, carbopol, gelatin, xanthan gum, natural cellulose, high cell, methyl cellulose, and combinations thereof. Emulsifying composition. The oil-in-water type emulsion according to claim 4, wherein the high melting point lipid comprises one selected from the group consisting of batyl alcohol, behenyl alcohol, cetostearium alcohol, cetyl alcohol, stearyl alcohol, and combinations thereof. Composition, The method of claim 4, wherein the high melting point lipid is batyl alcohol: behenyl alcohol: An oil-in-water emulsified composition comprising cetostearyl alcohol in a weight ratio of 1.0: 0.5: 0.5 to 1.0: 1.5: 2.0. The oil-in-water emulsion composition according to claim 4, wherein the low-melting lipid comprises one selected from the group consisting of mango butter, shea butter, copoa seed butter, macadamia seed oil, and combinations thereof. The method of claim 4, wherein the liquid oil is a hydrocarbon-based oil including polydecene and paraffin oil; Ester synthetic oils; Silicone oil; Animal and vegetable oils; Ethoxylated alkyl ester oils; cholesterol; Cholesteryl sulfate; Phytosphingosine; Sphingoidoid lipids; C10-40 fatty alcohol, caprylic / capric triglyceride, ceramide, and oil-in-water emulsion composition comprising one selected from the group consisting of a mixture thereof. The group of claim 4, wherein the liquid oil is a group consisting of macadamia seed oil, pentaerythritol tetraethylhexanoate, caprylic / capric triglycerides, vegetable squalane, hydrogenated seed 6-14 olefin polymer, and combinations thereof. Oil-in-water emulsion composition comprising one selected from. The oil-in-water emulsion composition according to claim 1, wherein the oil-in-water emulsion composition is a nano emulsion having a lipid capsule particle size of less than 300 nm. S1) preparing an aqueous phase including an amphiphilic lipid complex, a humectant, and purified water;
S2) preparing an oil phase including a high melting point lipid and a low melting point lipid on a liquid oil;
S3) adding a water phase to the oil phase to prepare a preemulsion;
S4) performing a high pressure emulsification process after cooling the preemulsion;
S5) A process for preparing an oil-in-water emulsion composition according to claim 1, which is performed by degassing after mixing the aqueous phase thickener and other additives with the obtained composition. A cosmetic composition comprising the oil-in-water emulsion composition of claim 1. 15. The cosmetic composition according to claim 14, wherein the cosmetic composition is a cosmetic composition selected from soft cosmetics, nourishing cosmetics, lotions, creams, packs, gels, or patches, lipsticks, makeup bases, or foundations. Cosmetic composition.

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