KR20120139310A - Oral composition for preventing or treating caries and periodontal disease - Google Patents

Abstract

PURPOSE: An oral composition containing Lonicera japonica thunberg extract is provided to ensure antibacterial and anti-inflammatory effects and to prevent and treat oral diseases and gingival diseases. CONSTITUTION: An oral composition for preventing and treating periodontal diseases contains 0.1-40 wt% of Lonicera japonica Thunberg extract as an active ingredient. The extract is a 70% ethanol extract, a hexane fraction, a dichloromethane(CH2Cl2) fraction, an ethyl acetate(EtOAc) fraction, a butanol fraction(BuOH) fraction, or a water(H_2O) fraction. The extract has an antibacterial effect against Fusobacterium nucleatum(ATCC 25586), Porphyromonas gingivalis(ATCC 33277), Treponema denticola(ATCC 35405), Tannerella forsythia(ATCC 43037), Aggregatibacter actinomycetemcomitans(ATCC 43718), or Prevotella intermedia(ATCC 25611). [Reference numerals] (AA) Anhuisheng Lonicera japonica Thunberg; (BB,DD,FF) Concentration(μg/ml); (CC) Hubei sheng Lonicera japonica Thunberg; (EE) Dongbei 3 sheng Lonicera japonica Thunberg

Description

Oral composition for preventing or treating caries and periodontal disease

The present invention relates to an oral composition for preventing and treating periodontal disease, and more particularly, to an oral composition for preventing and treating periodontal disease having an excellent anti-inflammatory and antimicrobial effect on periodontal disease, including the gold silver extract as an active ingredient.

Periodontal disease refers to an inflammatory state of tooth support tissue caused by bacteria, and can be separated into gingivitis and periodontitis. The cause is caused by oral bacteria due to poor oral hygiene to form dental plaque. Toothpaste bacterium refers to a mass of bacteria that grows after the bacteria adhere to the tooth surface by using a sticky substance in the saliva as an adhesive. If left untreated, the bacterial membranes are inflamed and sometimes bleed from the gums and cause bad breath. These symptoms are called gingivitis.

As the gingivitis progresses further, the gap between the teeth and the gums deepens and the periodontal sac develops, and the periodontal disease occurs due to the growth of bacteria causing the periodontal disease. As periodontitis progresses, even weak stimuli such as brushing may bleed and swell the gums, often turning into acute inflammation, causing pain. This inflammation lowers the function of making bones, and the bone absorbing effect is increased, the alveolar bone becomes lower and lower, and the alveolar bone is destroyed and eventually loses teeth.

Advanced periodontitis causes tooth loss. Tooth loss due to periodontitis is a serious problem in the adult population after 40 years of age, 40% of the causes of tooth extraction are reported.

The destruction of periodontal ligament and alveolar bone in periodontal disease is due to the degradation of collagen, a major component of these tissues. Although the exact mechanism is not yet known, the degradation of collagen is known to be caused by collagenase secreted by the host and neutral protease secreted by the causative agent of periodontal disease. Therefore, studies have been attempted to inhibit the progression of periodontal disease by inhibiting the activity of collagenase enzyme and protease.

The treatment of periodontal disease has been used for the establishment of improved oral hygiene, non-surgical or surgical calculus removal, root graft, gingival sopa and regeneration of periodontal tissue. However, this surgical treatment is cumbersome to go to the dentist for treatment, and most of the time to go to the chronic treatment when the treatment is not limited to the treatment that is performed to some extent rather than prevention of the disease.

As an additional treatment, systemic antibiotics and topical sustained release formulations have been used, but too much of the drug is delivered to unnecessary areas, resulting in side effects. In addition, recently, an example of isolated periodontal disease bacteria showing resistance to antibiotics has been reported has serious problems.

In order to supplement the limitations of the surgical treatment and the use of antibiotics, the use of oral compositions such as antibacterial, toothpaste containing anti-mortar preparations, and mouthwashes is an aid for the prevention and treatment of bacterial oral diseases such as dental caries and periodontal disease. The effect was reported as.

As a technology for treating periodontal disease targeting plant extracts, Korean Patent No. 138248 discloses a composition for enhancing oral hygiene containing a plant extract, and Korean Patent No. 121550 uses a pogongyoung for oral use. The composition is disclosed. In addition, Korean Patent No. 313491 relates to a toothpaste composition containing an antimicrobial plant extract, and discloses a composition for oral hygiene containing a vinegar, horse chestnut, pogongyoung, bamboo shoots, gall bladder, gold, white leaf, ginkgo biloba, etc. . However, the raw materials used in the above patents are not secured, and most of them are difficult to develop as a composition for oral hygiene. The development of oral hygiene products using raw materials that are not secured as in the prior arts has a problem that it is difficult to commercialize them in the near future due to the high ratio of time and material investment to prove the safety of the raw materials.

In order to solve the problems of the prior art as described above, an object of the present invention is to provide an oral composition for preventing and treating periodontal disease having excellent anti-inflammatory and antimicrobial effect against periodontal disease-causing bacteria.

It is another object of the present invention to provide an oral composition for preventing and treating periodontal disease having anti-inflammatory and antimicrobial effects against periodontal disease as well as caries, oral disease and gum disease.

In another aspect, the present invention is to provide a health food composition, quasi-drugs or pharmaceutical composition for periodontal disease having anti-inflammatory and antimicrobial effect against periodontal disease, caries, oral disease and gum disease.

In order to achieve the above object, the present invention provides an oral composition for preventing and treating periodontal disease, characterized in that it comprises 0.1 to 40% by weight of the extract of gold gilding as an active ingredient.

More preferably, the oral composition for preventing and treating periodontal disease of the present invention is to contain the gold and silver extract 10 to 20% by weight.

The sterling extract is 70% ethanol (EtOH) extract, hexane (hexane) fraction, dichloromethane (CH ₂ Cl ₂ ) fraction, ethyl acetate (EtOAc) fraction, butanol (BuOH) fraction or water (H ₂ O) fraction. desirable.

In particular, the gold coin extract is Fusobacterium nucleatum (ATCC 25586), Porphyromonas gingivalis (ATCC 33277), Treponema denticola (ATCC 35405), Tenerella Fort Excellent anti-inflammatory and antimicrobial effects against periodontal diseases of Tannerella forsythia ( ATCC 43037), Aggregatibacter actinomycetemcomitans (ATCC 43718) and Prevotella intermedia (ATCC 25611) Indicates.

The oral composition for preventing and treating periodontal disease as described above may be prepared in a formulation such as toothpaste, mouthwash, mouthwash, gum massage cream or edible film.

In another aspect, the present invention provides a health food composition for periodontal disease, characterized in that it comprises 0.1 ~ 40% by weight of the extract of the gold silver flower.

In another aspect, the present invention provides a quasi-drug or pharmaceutical composition for periodontal disease, characterized in that it comprises 0.1 ~ 40% by weight of the extract of gold silver.

The oral composition of the present invention has an excellent anti-inflammatory and antimicrobial effect against the causative bacteria of periodontal disease and at the same time has a great effect on the prevention and treatment of caries, oral disease and gum disease. In addition, the oral composition of the present invention is safe to the human body using natural extracts that have proven safety, and there are no side effects of use.

Figure 1 shows a 70% ethanol extract extracted from the gold (silver seongsui, Hubei, Northeast 3 star) gold and silver coins according to an embodiment of the present invention.
Figure 2 shows a process for separating the fractions and the separated fractions from the safe gold silver coin according to an embodiment of the present invention.
Figure 3 shows a process for separating fractions and separated fractions from Hubei gold and silver coins according to an embodiment of the present invention.
Figure 4 shows a process for separating the fractions from the northeastern three gold and silver coins according to an embodiment of the present invention and the separated fractions.
Figure 5 is a graph showing the antimicrobial test results of the gold-silver extract of each region for periodontal disease-causing bacteria (Pugeotbacterium nucleatum) according to an embodiment of the present invention.
Figure 6 is a graph showing the antimicrobial test results of the extract of the gilding silver for each region against periodontal disease causing bacteria (porphyromonas gingivalis) according to an embodiment of the present invention.
Figure 7 is a graph showing the results of the antibacterial test of the gold-silver extract of each region for periodontal disease-causing bacteria (treponema denticola) according to an embodiment of the present invention.
Figure 8 is a graph showing the antimicrobial test results of the gold silver extract of each region for periodontal disease-causing bacteria (Tenella Forsythia) according to an embodiment of the present invention.
Figure 9 is a graph showing the results of cytotoxicity test for 70% ethanol extract (solid content) of the gold and silver extract of each region for gingival fibroblasts according to one embodiment of the present invention.
Figure 10 is a graph showing the cytotoxicity test results for the 70% ethanol extract (solid content) of the gold and silver extract of each region for osteoblasts according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

The present inventors verified direct antimicrobial activity against several anaerobic bacteria causing periodontal disease, and verified that gold and silver extracts had growth inhibition ability against the periodontal disease-causing bacteria, and confirmed that the present invention can be used as a treatment for periodontal disease. It was completed.

The composition for preventing and treating periodontal disease of the present invention is characterized in that it comprises a gold silver extract as an active ingredient.

The Lonicera japonica thunberg refers to a bud of a honeysuckle or a variant thereof. Cold and nonpoisonous, sweet taste When you have a fever with an external feeling and you feel stuffy in your chest and have uncomfortable symptoms and thirst, drink it by car. It is effective when the boil and skin are torn and the poison spreads, and it removes inflammation and pus from appendicitis, peritonitis, endometritis and internal organ inflammation. It is also effective when excreting feces mixed with dysentery.

The silver coin has a rapid effect on the tissue necrosis of the skin due to heat poisoning, the treatment rate is high even in the early mastitis. It lowers the heat, loosens the poison and makes the meridians pass through. It has strong antibacterial effect, which is applied to various inflammatory diseases, fever due to cold, and surgeon's swelling. It is effective for epidemic or malignant pandemic, respiratory disease, syphilis due to virus suppression effect, and is also used for unconsciousness or hand and hand paralysis due to high fever, sore throat, colitis, gastric ulcer, cystitis, pharyngitis, tonsillitis, bronchitis, conjunctivitis and Treat swelling, high fever caused by mumps, and purulent infections. In addition, it has antibacterial activity, inhibits the growth of Staphylococcus aureus, hemolytic Streptococcus, pneumococcal pneumoniae, pertussis, dysentery, Pseudomonas aeruginosa, cholera and Escherichia coli, and is effective in anti-inflammatory and antipyretic action Promote the phagocytosis of white blood cells. Serum cholesterol is lowered consistently and lightly prevents experimental ulcers.

As the gold and silver coins, a normal one or a commercially available one can be purchased and used, of course, in the present invention, those produced in Anhui, Hubei, and Northeastern three provinces of China were used.

The sterling silver extract can be extracted according to a conventional extraction method such as cold needle, warm needle, heating using a solvent such as water and alcohol.

More specifically, the sterling silver extract is first refluxed at 70 ° C. with 70% ethanol (EtOH) and extracted five times for 3 hours to prepare 70% ethanol extract. Then, suspended by adding distilled water to the 70% ethanol extract, the same amount of hexane (hexane) was added to the mixture, left to shake, and fractionated to separate the hexane fraction and the water layer, and then the same amount of dichloromethane (CH ₂ Cl ₂ ), ethyl acetate (EtOAc) and butanol (BuOH) were added sequentially and fractionated according to the same method to separate the dichloromethane fraction, ethyl acetate fraction and butanol fraction, and then the aqueous layer was concentrated to give a water (H ₂ O) fraction. You can get it.

The gold and silver is preferably contained in 0.1 to 40% by weight, more preferably 10 to 20% by weight in the oral composition for preventing and treating periodontal disease of the present invention. If the content is less than 0.1% by weight can not see the anti-inflammatory and antimicrobial effect on as many periodontal disease-causing bacteria as desired, if more than 40% by weight of the anti-inflammatory and antimicrobial effect according to the amount used is small, prepared as an oral treatment Can be difficult.

Oral bacteria include Porphyromonas gingivalis , Fusobacterium nucleatum , Prevotella intermedia , Prevotella loescheii , Triponema denticola ( Treponema denticola ), Porphyromonas endodontalis , Peptococcus anaerobius , Micros prevotii , Eubasterium limosum , Centipedia perio Centipedia periodontii , Selenomonas arterimidis , Fusobacterium periodonticum , Eubacterium spp. , Bacteroides spp. , as the bacteria involved in periodontal disease, fatigue Fort Monastir long lease Bangui (Porphyromonas gingivalis), Peugeot tumefaciens New Lea Tomb (Fusobacterium nucleatum), liquid Martino Bacillus liquid Martino Mai setem Cormier Tansu (Actinobacillus actinomycetemcomitans), Kam fatigue bakteo selector switch (Campylobacter rectus), tene Pasteurella formate when thiazol (Tennerella forsythia), Trail Four nematic denti coke (Treponema denticola) , Prevotella intermedia , Actinomyces Viscosos , Streptococcus mutans , Streptococcus sobrinus .

Among these bacteria, antimicrobial agents that are particularly active against Porphyromonas gingivalis and Fusobacterium nucleatum are believed to be effective in reducing oral bacteria.

Porphyromonas gingivalis is a Gram-negative anaerobic bacterium that is representative of periodontal causative bacteria and is known as a causative agent of bad breath by producing VSC. Porphyromonas gingivalis has strong adhesion and co-aggregates with a plurality of fungal species (published first dental publication, quarterly dental care 20 (1) 31-39, 2006). In addition, porphyromonas gingivalis produces proteases such as collagenase and gingifen, and superoxide dismutase, thereby inhibiting the host's immune response. It is also reported that Ginaffein, which is secreted by Porphyromonas gingivalis, contributes to the coagulation of Actinomyces viscous.

Peugeot bacterium nucleatum is also a Gram-negative anaerobic bacterium and is known to be a causative agent of periodontal disease and to produce VSC and to cause bad breath. Peugeot bacterium nucleatum produces fatty acids that are toxic to periodontal tissue bacteria, inhibits the host's immune response and further promotes the growth of spiroheta and the like.

As described above, Porphyromonas gingivalis and Peugeot bacterium nucleatum inhibit the host's immune response, promote the growth of other oral bacteria and contribute to the formation of plaque.

In the present invention, in order to verify the antimicrobial effect of the sterling silver extract on the causative bacteria of periodontal disease, 70% ethanol (EtOH) extract, hexane (hexane) fraction, dichloromethane (CH ₂ Cl ₂ ) fraction, ethyl of the gold sinter isolated as described above Fusobacterium nucleatum (ATCC 25586), Porphyromonas gingivalis , the cause of periodontal disease, using acetate (EtOAc), butanol (BuOH) and water (H ₂ O) fractions ATCC 33277), Treponema denticola (ATCC 35405), Tannerella forsythia ( ATCC 43037), Aggregatibacter actinomycetemcomitans (ATCC 43718) and Prebotela Growth inhibition against intermedia ( Prevotella intermedia , ATCC 25611) was tested.

Oral composition for preventing and treating periodontal disease of the present invention as described above is effective in periodontal disease, such as gingivitis, periodontitis, not only reduce the inflammation of the gums but also antibiotics to resist from bacterial infection by strengthening the gums. To be able.

The oral composition for preventing and treating periodontal disease of the present invention is not particularly limited in the formulation and may have a conventional formulation, and specifically, formulations such as toothpaste, mouthwash, mouthwash, gum massage cream, and edible film. Can have The oral composition provided by the present invention may contain various bases and additives necessary for formulation according to the formulation, and the type and amount of these components can be easily selected by those skilled in the art. For example, when the formulation of the oral composition is a toothpaste, it may be prepared by adding an abrasive, a humectant, a foaming agent, a binder, a sweetening agent, a pH adjusting agent, a preservative, an active ingredient, a flavoring agent, a whitening agent, a pigment, a solvent, and the like.

In another aspect, the present invention provides a health food composition, quasi-drugs or pharmaceutical compositions for the purpose of improving periodontal disease, including the above-mentioned sterling silver extract as an active ingredient.

In the case of using the silver coin extract of the present invention as a food additive, the silver coin extract may be added as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). In general, in the preparation of food or beverages, the sterling extract of the present invention is added in an amount of 50 parts by weight or less, preferably 25 parts by weight or less based on the raw material. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. Examples of foods to which the substance may be added include meat, sausages, bread, chocolate, candy, jelly, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, drinks, tea , Drinks, alcoholic beverages, vitamin complexes, etc., and includes all of the health food in the usual sense.

When the sterling silver extract of the present invention is used as a beverage, it may contain various flavors or natural carbohydrates, etc. as additional ingredients, as in general beverages. The above-mentioned natural carbohydrates are sugars such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as string and cyclodextrin, xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The proportion of the natural carbohydrate is generally about 0.1-20 g, preferably about 1-10 g per 100 ml of the composition of the present invention.

In addition to the above, when the silver coin extract of the present invention is used as a beverage, various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH regulators, stabilizers, Preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. In addition, when the sterling extract of the present invention is used as a beverage, it may contain a flesh for preparing natural fruit juice, fruit juice beverage and vegetable beverage. These components can be used independently or in combination. The ratio of such additives is not critical, but is generally selected in the range of 0.05 to 50 parts by weight per 100 parts by weight of the beverage containing the gold coin extract of the present invention.

Hereinafter, the present invention will be described in more detail with reference to examples. These embodiments are for purposes of illustration only and are not intended to limit the scope of protection of the present invention.

Example 1 Preparation of Gold Coin Extract

1-1. 70% Ethanol Extract

15 g of gold and silver coins purchased from Anhui, Hubei, and Northeast China were refluxed at 70 ° C. with 70% ethanol (EtOH), and extracted three times for 5 hours to obtain 70% ethanol extract. The gold coins thus obtained were 8.3 g, 4.7 g and 7.6 g, respectively.

Among the 70% ethanol extracts obtained as described above, the highest yield was the anhydrous gold and silver coins, and the weight of the solids was 300 mg, and the weight of the solids was 100 mg for the Hubei gold and the northeast gold coins respectively (Fig. 1). ).

1-2. Gold and Silver Fraction Separation

20 g of gold and silver coins purchased from Anhui, Hubei, and Northeast China, were refluxed at 70 ° C. with 70% ethanol (EtOH), and extracted five times for 3 hours to obtain 70% ethanol extract.

Distilled water was added to the 70% ethanol extract of the gold and silver coins thus obtained, and it suspended and adjusted to 200 ml. The same amount of hexane (hexane) was added thereto, and the mixture was left to stand for shaking, and then fractionated to separate the hexane fraction and the aqueous layer. The same amount of dichloromethane (CH ₂ Cl ₂ ) was added to the aqueous layer, followed by shaking. Was separated. In addition, the same amount of ethyl acetate (EtOAc) was added to the separated aqueous layer, and the mixture was left to shake and fractionated to separate the ethyl acetate fraction and the aqueous layer. Subsequently, butanol was added to the aqueous layer, and the mixture was left to shake. The mixture was separated, the butanol fraction was separated from the aqueous layer, and the aqueous layer was concentrated to obtain a water (H ₂ O) fraction.

Hexane (hexane) fraction, dichloromethane (CH ₂ Cl ₂ ) fraction, ethyl acetate (EtOAc) fraction, butanol (BuOH) fraction and water (H) ₂ O) The separation process of the fractions and the fractions thus obtained are shown in Figures 2 to 4.

In addition, the weight of the fractions for 20g gold silver coins by each region (anseong, Hubei, Northeast 3 star) was measured and shown in Table 1 below.

The units in Table 1 are g.

division Anseong Gold Coin Hubei gold and silver coins Northeast 3 Star Gold and Silver Coin Hexane fraction 0.024 0.071 0.032 Dichloromethane fractions 0.122 0.148 0.118 Ethyl acetate fraction 0.357 0.295 0.398 Butanol fraction 4.28 3.07 3.53 Water fraction 3.536 1.83 3.452 Sum 8.319 5.414 7.53 recovery rate (%) 75.63 87.32 75.30

Example 2. Antibacterial Test

In order to measure the effect of preventing and treating periodontal disease of the sterling silver extract, 70% ethanol extract, hexane fraction, dichloromethane (CH ₂ Cl ₂ ) Antibacterial test was performed on the causative bacteria of periodontal disease using fractions, ethyl acetate (EtOAc) fractions, butanol (BuOH) fractions and water (H ₂ O) fractions.

Periodontal disease causes include Fusobacterium nucleatum (ATCC 25586), Porphyromonas gingivalis (ATCC 33277), Treponema denticola (ATCC 35405), and Tenerella forsythia. Tannerella forsythia ( ATCC 43037), Aggregatibacter actinomycetemcomitans (ATCC 43718) and Prevotella intermedia (ATCC 25611) were used.

Eggrigatibacter actinomycetemcomitans is in a brain-heart infusion (BHI) liquid medium, while Peugeot bacterium nucleatum, pribotella intermedia, and porphyromonas gingivalis are BHI in hemin and vitamin K. In liquid medium containing (vitamin K), Treponema Denticola was mixed with TYGVS and BHI in a 1: 1 ratio, and Tenenella Porcitia was added to media using modified NOS (New Oral Spirochete) media. Diluted to a concentration of × 10 ⁶ cells / ㎖ and inoculated with 150 μl, pre-incubated at 37 ℃ for 24 to 48 hours.

First, the hexane fraction, dichloromethane fraction and ethyl acetate fraction were dissolved in anhydrous alcohol, the remaining 70% ethanol extract, butanol fraction and water fraction was dissolved in distilled water, and then filtered and sterilized with a 0.2㎛ filter to prepare a sample.

Each sample was diluted using serial dilution on a microtitre plate, and each sample was inoculated with a final volume of 150 μl of the pre-cultured periodontal causative bacteria. These were incubated at 37 ° C. for 24 to 48 hours under anaerobic conditions, and then absorbed at 650 nm with an Enzyme-Linked Immunosorbent Assay (ELISA) to test their growth inhibition ability against the causative agent of periodontal disease. 5 to 8 shows the results of the antibacterial test of the gold and silver extract of each region for each periodontal disease-causing bacterium.

As shown in Figures 5 to 8, the gold and silver extract of the present invention and each fraction is the cause of periodontal disease, Peugeot bacterium nucleatum (Fig. 5), Porphyromonas gingivalis (Fig. 6), treponema denti It was confirmed that the antimicrobial activity was very excellent against coke (FIG. 7) and Tennessella porcitia (FIG. 8).

Through these results, it was predicted that the sterling silver extract contained in the oral composition of the present invention would be effective in preventing and treating periodontal disease by inhibiting growth of periodontal disease causing bacteria.

Example 3. Cytotoxicity Test

To test the cytotoxicity of the sterling silver extract, gingival fibroblasts and osteoblasts were cultured under the following conditions and used for the test.

Gingival fibroblast cell lines were used to treat supercultured human GF cells with 10% FBS (PAA Laboratories, Etobicoke, ON, Canada), DMEM high glucose medium supplemented with 1% penicillin / streptomycin (PAA Laboratories, Linz, Incubated at 37 ° C., 5% carbon dioxide, and 99% humidity until 80% confluence was reached. The medium was changed every three days. As osteoblast like cell line, MG63 (ATCC, Manassas, VA, USA) was cultured in the same DMEM medium as the gingival fibroblasts.

First, 70% ethanol extract (solid content) of anisotropic, Hubei, and northeastern gold and silver coins obtained in Example 1 was dissolved in DMSO, and filtered and sterilized using a 0.2 μm cellulose filter.

The prepared gingival fibroblasts and osteoblasts were cultured in 96 wells so that 3 × 10 ³ cells per well were obtained. After 24 hours of incubation was changed to free media. Then, 70% ethanol extracts of the anisotropic, Hubei, and northeastern three silver coins were added at a concentration of 1, 5, 10, 50, 100 ㎍ / ml per well, and then incubated at 37 ° C. for 24 to 72 hours under anaerobic conditions. Thereafter, cell line survival results (survival cell numbers) according to the experimental time obtained through the MTT assay (540 nm) are shown in FIGS. 9 and 10. The experimental result is an average value from repeated experiments.

As shown in Figures 9 and 10, when the gingival fibroblasts were treated with 70% ethanol extracts of ophthalmic, Hubei, and northeastern three, the survival rate of the cells was remarkably increased over time compared to the control without the treatment of the sterling silver extract. It was found from these results that the gilding extract contained in the oral composition of the present invention is excellent without cytotoxicity to gingival fibroblasts (FIG. 9) and osteoblasts (FIG. 10).

Preparation Example 1 Toothpaste

48.0 wt% calcium carbonate, 20.0 wt% glycerin, 2.0 wt% carboxymethylcellulose, 2.0 wt% sodium lauryl sulfate, 1.0 wt% fragrance, 0.1 wt% saccharin, 3.0 wt% anhydrous gold silver 70% ethanol extract of Example 1 , 0.01% by weight of chlorohexidine and the residual amount of water were mixed to prepare a toothpaste having the effect of preventing and treating periodontal disease according to a conventional toothpaste manufacturing method.

Preparation Example 2 Preparation of Mouth Cleaning Agent (Gagle)

0.1% by weight of safety gold silver ethanol extract of Example 1, glycerin 35.5% by weight, polyacrylate sodium 5.0% by weight, sodium lauryl sulfate 1.0% by weight, saccharin sodium 0.1% by weight, ethanol 5.0% by weight, flavor 1.3 By mixing the weight percent and the residual amount of water to prepare a mouthwash (gagle) having the effect of preventing and treating periodontal disease according to a conventional mouthwash preparation method.

Preparation Example 3 Preparation of Oral Spray

0.1% by weight of safety gold silver ethanol extract of Example 1, glycerin 35.5% by weight, polyacrylate sodium 5.0% by weight, sodium lauryl sulfate 1.0% by weight, saccharin sodium 0.1% by weight, ethanol 5.0% by weight, flavor 1.3 By mixing the weight percent and the residual amount of water to prepare an oral spray having a periodontal disease prevention and treatment according to the conventional oral spray manufacturing method.

Preparation Example 4 Chewing Gum Preparation

Preparation of chewing gum by mixing 20.0% by weight of gum base, 54.7% by weight of sugar, 15.0% by weight of glucose, 9.3% by weight of starch syrup, 0.5% by weight of fragrance, and 0.5% by weight of safety gold and silver coin 70% ethanol extract of Example 1 According to the method was prepared chewing gum that has the effect of preventing and treating periodontal disease without losing the properties of chewing gum.

Preparation Example 5 Candy Preparation

Sugar 49.6% by weight, starch syrup 33.0% by weight, citric acid 1.0% by weight, flavor 0.2% by weight, L- menthol 1.0% by weight, 0.8% by weight of anhydrous gold and silver ethanol extract of Example 1 and 14.4% by weight of water Chewing gum having an effect of preventing and treating periodontal disease was prepared according to a conventional candy manufacturing method.

Preparation Example 6 Chocolate Preparation

Cacao butter 20.0% by weight, whole milk powder 20.0% by weight, cacao butter 17.0% by weight, 41.75% by weight, lecithin 0.45% by weight, fragrance 0.2% by weight and 0.6% by weight of anhydrous gold and silver ethanol extract of Example 1 To prepare a chocolate having a periodontal disease prevention and treatment according to the conventional chocolate production method.

Preparation Example 7 Beverage Preparation

Orange juice 30.0% by weight, isomerized sugar 15.33% by weight, citric acid 0.1% by weight, vitamin C 0.04% by weight, fragrance 0.1% by weight, 0.01% by weight of anhydrous gold and silver ethanol extract of Example 1 and 54.42% by weight of water To prepare a beverage having the effect of preventing and treating periodontal disease according to the conventional beverage production method.

Preparation Example 8 Preparation of Edible Film

To prepare a edible film having the effect of preventing and treating periodontal disease by containing 10 to 20% by weight of the gold gilde extract in a conventional edible film.

Although the present invention has been described as the preferred embodiment mentioned above, it is possible to make various modifications or variations without departing from the spirit and scope of the invention. The appended claims also cover such modifications and variations as fall within the spirit of the invention.

Claims (7)

Oral composition for preventing and treating periodontal disease, characterized in that it comprises 0.1 to 40% by weight of the gold silver extract as an active ingredient. The method of claim 1,
The silver coin extract oral composition for preventing and treating periodontal disease, characterized in that it comprises 10-20% by weight in the oral composition. The method of claim 1,
The sterling extract is any one of 70% ethanol (EtOH) extract, hexane (hexane) fraction, dichloromethane (CH ₂ Cl ₂ ) fraction, ethyl acetate (EtOAc) fraction, butanol (BuOH) fraction and water (H ₂ O) fraction Oral composition for preventing and treating periodontal disease, characterized in that one. The method of claim 1,
The silver coin extract is Fusobacterium nucleatum (ATCC 25586), Porphyromonas gingivalis (ATCC 33277), Treponema denticola (ATCC 35405), Tenerella porcitia ( Tannerella forsythia, ATCC 43037), Aggregatibacter actinomycetemcomitans (ATCC 43718) and Prevotella intermedia (ATCC 25611) have antibacterial activity against the causative agent of periodontal disease Oral composition for preventing and treating periodontal disease, characterized in that. The method of claim 1,
Oral composition for preventing and treating periodontal disease, characterized in that the formulation of the composition is a toothpaste, mouthwash, mouthwash, gum massage cream or edible film. Gold functional flower composition for periodontal disease, characterized in that containing 0.1 ~ 40% by weight as an active ingredient. Quasi silver extract or quasi-drugs or pharmaceutical compositions for periodontal disease, characterized in that it comprises 0.1 to 40% by weight as an active ingredient.

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