KR20050075906A - Precess for eliminating anthraquinone from leaves of aloe vera - Google Patents

Abstract

The present invention relates to a method for removing anthraquinone from fresh aloe vera leaves, and more particularly, to a method for removing anthraquinone from fresh aloe vera leaves by applying a temperature shock to the fresh aloe vera leaves.

Description

Preservation for eliminating anthraquinone from leaves of aloe vera}

The present invention relates to a method for removing anthraquinone from fresh aloe vera leaves, and more particularly, to a method for removing anthraquinone from fresh aloe vera leaves by applying a temperature shock to the fresh aloe vera leaves.

Aloe vera is a botanist and is a perennial herb of the genus Aloe vera of the family Liliaceae. It is native to Africa and now grows widely in tropical and temperate regions, and has become a world-class medicinal plant. Currently used aloe vera is Aloe vera Linne (Aloe bradesis Miller) and Aloe vera aborescens (Aloe arborescens). Aloe vera is not only toxic and no side effects, but also heals a variety of conditions to solve problems such as various diseases, X-ray irradiation.

Aloe vera contains aloe vera that acts on each gut, including gastrointestinal tract and digestive system, aloe vera emodine, a polyalkylate having a molecular weight of about 450,000, which has fluid improvement and anticancer activity as a strong alkyl, and aloe veratine that has antibacterial activity, ulcers It contains various components, such as aloe veraulsin, which acts on, and aomycin, which has antitumor antiviral action.

Due to the various components of aloe vera, aloe vera is bactericidal, anti-ulcer cell reactivity, cardiac blood circulation, cholesterol lowering, visceral function and endocrine stabilization, dry stomach edible enhancement , Anti-cancer action, body fluid alkalizing action, collagen improvement, skin beauty action, detoxification action, history-resistant anti-inflammatory action, anti-allergic antihistamine action, etc. It is a state.

Referring to a conventional method for preparing aloe vera gel, the raw aloe vera leaves are first washed, the outer shell is removed, and then the anthraquinone is removed from the aloe vera gel.

As a method for removing anthraquinone from the aloe vera gel, "a method of preparing aloe vera gel containing fiber using ionized water (Korean Patent Publication No. 1997-0009619)", "a gel is removed from the vera leaf. Separating and adding vitamin C stabilized aloe vera juice preparation method (Korean Patent Publication No. 1996-0011516), "Aloe vera fruit granules using only the flesh part of the aloe vera leaf completely removed Contained functional drink and its manufacturing method (Korean Patent Publication No. 1996-0036957), "" Cutting the bottom of the washed aloe vera leaf, and then cut it horizontally 20 ~ in hypertonic, isotonic, hypotonic aqueous solution Aloe vera extract obtained by leaching for 30 minutes and preparation method thereof (Korean Patent Publication No. 1993-1062), "Aloe vera gel solution in which aloe is prepared by immersing aloe vera gel in ethanol. This production method, etc. (Korea Patent Publication No. 1996-104316 No.) "are known.

However, when the anthraquinone is removed from the aloe vera gel as described above, there is a problem in that the content of polysaccharides is lower than that of the anthraquinone from the raw aloe vera leaves.

Therefore, the present inventors confirmed that removing anthraquinone from raw aloe vera leaves rather than aloe vera gel can increase polysaccharide content, and found a method of removing anthraquinone from raw aloe vera leaves by applying a temperature shock to the fresh aloe vera leaves. The present invention has been completed.

Accordingly, it is an object of the present invention to provide a method for removing anthraquinone from fresh aloe vera leaves by subjecting the fresh aloe vera leaves to temperature shock.

In the present invention, the upper and lower portions of the washed aloe vera leaf is cut and then immersed in distilled water at 20 to 60 ℃ for 5 to 30 minutes to remove the anthraquinone.

The specifications for aloe gel products in the dietary supplements regulate the anthraquinone content to 0.005% or less. Anthraquinones in aloe vera are known as browning agents and are also known to be extremely irritant laxatives.

Accordingly, the present invention provides a method for removing anthraquinone from raw aloe vera leaves but not aloe vera gel.

Anthraquinone removal process according to the present invention is as follows:

1) collecting and washing the fresh aloe vera leaves;

Aloe Vera leaves grown for more than 3 years are collected and the surface of the fresh leaves is washed with water.

2) partially cutting the aloe vera leaf; And

The upper and lower parts of the fresh aloe vera leaves are cut in a conventional manner.

3) dipping the aloe vera leaf in distilled water to remove the anthraquinone

The cut raw leaves are immersed in a water bath containing 20 to 60 ° C. of distilled water to remove anthraquinones. At this time, in the case of the peeled raw leaves, the water-soluble effective ingredients are more likely to escape because the contact surface with the distilled water is large, but the aloe fresh leaves cut in the present invention do not escape the water-soluble active ingredients because the contact surface between the distilled water and the aloe gel is small.

[Example 1] A method for preparing an aloe gel by removing anthraquinone from fresh aloe leaves

3 kg of aloe vera leaves grown for more than 3 years were collected and the surface of fresh leaves was washed with water. The top and bottom portions of the washed aloe vera leaves were cut to a constant size in a conventional manner. The raw aloe vera leaf was cut into a water bath containing 500 ml of distilled water, and the temperature was set to room temperature (20 ° C.), followed by immersion for 30 minutes. After removing the outer skin of the deposited aloe vera leaves, aloe gel was prepared by pulverizing twice with a green juice.

Comparative Example 1 A method for preparing aloe vera gel

3 kg of aloe vera leaves grown for more than 3 years were collected and the surface of fresh leaves was washed with water. The washed aloe vera fresh leaves were peeled and placed in a water bath containing 500 ml of distilled water, and then the temperature was set to room temperature (20 ° C.), and then immersed for 30 minutes. The deposited aloe gel was ground twice with a green juice to prepare an aloe gel.

[Comparative Example 2] Aloe leaves raw raw leaves

Aloe was prepared in the same manner as in Example 1, except that the upper and lower portions of the fresh leaves were peeled off without being cut.

[Test Example 1] Comparison of Anthraquinone Content by Deposition

<Method of measuring anthraquinone content>

1. Test apparatus

Reflux condenser, water bath, 500 ml aliquot, 300 ml beaker, funnel, filter paper, 50, 100, 250 ml measuring cylinder, 250 ml water, agitator, 1, 5 and 10 ml pipette, spectrophotometer, filter no.2 (110 mm)

2. Reagent

Ferric chloride, concentrated hydrochloric acid, sodium hydroxide, carbon tetrachloride, methanol, magnesium acetate

3. Test method

500 ml of distilled water was put into a water bath, and the temperature was set to 60 degreeC. 50 g (S) of Aloe Vera leaves of Example 1 and Comparative Examples 1 and 2 were each slightly taken by hand into a 250 ml beaker. 10 ml of methanol and 10 ml of 60 ° C. distilled water were added, followed by stirring for 30 seconds. Then, 75 ml of 60 ° C. distilled water was added thereto, followed by stirring in a stir 3.5 for 30 minutes, followed by standing at room temperature. The oriental filter was laid, and naturally filtered using a funnel, and distilled water was added to the filtrate to obtain a volume of 200 ml. 10 ml of the filtrate was placed in a fat water, 1 ml of 60% ferric chloride solution and 6 ml of concentrated hydrochloric acid were mixed, and a reflux cooler was installed in the water. Water was put into an aluminum pan using a gas burner and heated for 4 hours. After cooling with water, the solution was transferred to a separatory filter, and the cooler and the water were washed with 10 ml of distilled water, and the tax solution was added to the separatory filter. 4 ml of 1N NaOH was added, and extracted repeatedly with 20 ml of carbon tetrachloride. The carbon tetrachloride layer was washed with 10 ml of distilled water and carbon tetrachloride was added to make the volume 100 ml. 20 ml of the filtrate was evaporated to dryness in a 100 ml beaker. 10 ml of 0.5% methanol magnesium acetate solution was added to the residue to dissolve it, and used as a test solution. The absorbance was measured using methanol as a reference solution at a wavelength of 512 nm of 1 cm of the dissolved liquid layer.

In order to determine the content of the anthraquinone remaining in the aloe vera gel using the absorbance measured by the above method was calculated in the same manner as in Equation 1 below.

S: Amount of fresh aloe vera leaves or gel of Example 1 and Comparative Examples 1 to 2 g

A: absorbance

<Result>

Table 1 shows the results of comparing and measuring the anthraquinone content contained in the aloe vera gel of Example 1 and Comparative Examples 1 and 2.

Comparative Example 1 Comparative Example 2 Example 1 Anthraquinone Content (%) 0.0025 0.003 0.0015

[Test Example 2] Comparison of the anthraquinone content according to the change of deposition temperature

After varying the deposition temperature, the anthraquinone content of the aloe vera gel prepared by removing anthraquinone from the freshly cut aloe vera leaves in the same manner as in Example 1 was measured in the same manner as in Test Example 1. Is shown in Table 2 below.

Deposition temperature change Anthraquinone Content (%) Deposition at room temperature (immersion time 30 minutes) 0.0015 15 minutes at 28 ° C, 15 minutes at 38 ° C 0.0013 Immersion 30 minutes at 15 ℃ 0.0015 Immersion at 50 ° C for 7 minutes 30 seconds 0.0007 5 minutes at 60 ° C 0.0004

As can be seen from the above results, it was found that the content of anthraquinone decreased as the deposition temperature increased, and thus, more anthraquinone was removed from the aloe vera leaves.

[Test Example 3] Comparison of the anthraquinone content according to the change in deposition time

After varying the deposition time, the anthraquinone content of the aloe vera gel prepared by removing anthraquinone from the freshly cut aloe vera leaves in the same manner as in Example 1 was measured in the same manner as in Test Example 1. It is shown in Table 3 below. At this time, the temperature of the distilled water is 50 ℃.

Deposition time 7 minutes 30 seconds 15 mins 30 minutes Anthraquinone Content (%) 0.0007 0.0005 0.0004

As can be seen from the above results, the longer the deposition time was found to decrease the content of anthraquinone, and thus it can be seen that the anthraquinone was most removed from the fresh leaves of Aloe Vera when the deposition time was more than 30 minutes.

[Test Example 4] Comparison of the anthraquinone content according to the change in deposition time

After varying the deposition time, the anthraquinone content of the aloe vera gel prepared by removing anthraquinone from the freshly cut aloe vera leaves in the same manner as in Example 1 was measured in the same manner as in Test Example 1. It is shown in Table 4 below. At this time, the temperature of the distilled distilled water is 60 ℃.

Deposition time 5 minutes 7 minutes 30 seconds 10 minutes Anthraquinone Content (%) 0.0004 0.0003 0.00007

As a result of Test Example 3, it was found that the content of anthraquinone decreases as the deposition time increases.

Test Example 5 Measurement of Polysaccharide Loss

In order to determine the degree of polysaccharide loss of the aloe vera gel prepared in Examples 1 and Comparative Examples 1 and 2, the average molecular weight of each aloe vera gel was measured using SE C-MALLS-RI Analysis Conditions. Table 5 shows.

Comparative Example 1 Comparative Example 2 Example 1 Average molecular weight (g / mol) 539,000 1,215,000 1,210,000

Test Example 6 Comparison of Acetyl Group Content by Deposition

<Method of measuring acetyl group content>

1. Test apparatus

UV spectrometer

2. Reagent

Sodium hydroxide [NaOH], HydroxylamineHCl [(NH ₃ OH) Cl], Iron chloride [FeCl ₃ · 6H ₂ O], Acetyl choline chloride [C ₇ H ₁₆ ClNO ₂ ], Sodium acetate [CH ₃ COONA · 3H ₂ O]

3. Test method

Acetyl group was quantified as follows by Hestrin's method. 1 ml of the sample was added to the test tube, and 2 ml of 1: 1 (v / v) of hydroxyamine reagent (2M Hydroxylamine HCl: 3.5N NaOH) was added. After 1 minute, 1 ml of a solution diluted twice with distilled water of conc. The pH was about 1.2, and 10 g of Iron chloride [FeCl ₃ · 6H ₂ O] was dissolved in 0.1 N HCl, and 1 ml of iron reagent (100 ml) was added and centrifuged at 300 rpm, and the absorbance was measured at 450 nm. The acetyl group was quantified using Equation 2.

`` At: absorbance of the sample

Cs: concentration of standard solution

Ma: acetyl group molecular weight

As: absorbance of standard sample

Ws: weight of sample

Vs: volume of sample

Mucopolysaccharides of aloe contained in aloe consist of a mixture of polysaccharides, and "polysaccharides" are used to include both oligomers and polymers of carbohydrates. This polysaccharide group is named acetylmannan. This patent measured the acetyl group as part of the determination of aloe acetylmannan (regular linear polymer of acetylated mannose monomer), and the results are shown in Table 6 below.

Comparative Example 1 Comparative Example 2 Example 1 Acetyl group content (%) 28.59 44.10 43.23

As described above, in the present invention, in order to remove the anthraquinone while reducing the polysaccharide loss of the aloe vera, anthraquinone can be removed while the polysaccharide loss is less by partially cutting the raw leaves of the aloe vera rather than the aloe vera gel. there was.

Claims (2)

1) collecting and washing; 2) partially cutting; And 3) immersing the fresh aloe vera leaves in distilled water; Containing, Method for removing anthraquinone from aloe vera raw leaves. The method of claim 1, wherein the distilled water immersion step is characterized in that the aloe vera raw leaves are immersed in distilled water at 20 ~ 60 ℃.