KR20030021298A - The composition of immune enhancer and intestinal regulator for animal - Google Patents

Abstract

The present invention relates to an animal immunostimulating and formal composition comprising ascorbic acid-treated stabilizing wood vinegar and chitin and lactic acid bacteria culture as a main component, to increase the immunity of the animal when fed to reduce the damage caused by various diseases, The present invention relates to an animal immunostimulating and dressing composition for improving the productivity of the animal by keeping the intestine healthy and improving feed efficiency.

Description

Immunity Enhancement and Formal Composition for Animals {THE COMPOSITION OF IMMUNE ENHANCER AND INTESTINAL REGULATOR FOR ANIMAL}

The present invention relates to an animal immunostimulating and formal composition comprising ascorbic acid-treated stabilizing wood vinegar and chitin and lactic acid bacteria culture as a main component, more specifically Korean Patent Application No. 10-2001-0050219 (ascorbic acid-treated stabilizing wood vinegar) And ascorbic acid-treated stabilizing wood vinegar and tyndallized lactic acid bacteria culture, which are disclosed in the disinfectant composition containing the same, as a main ingredient, to enhance the disease of the disease by enhancing the immune system of animals, strengthening the history of the disease, and suiting the digestive organs. The present invention relates to a composite composition for animal immunostimulation and formal use for the purpose of preventing the occurrence and speeding up recovery in the event of a disease, to reduce the damage.

Wood vinegar is a crude wood vinegar (crude wood vinegar) obtained by cooling the smoke produced when wood carbonizes (combustion temperature 80 ℃ ~ 150 ℃ for conventional charcoal kiln, below 150 ℃ for smoke collection temperature for mechanical). The crude wood vinegar is allowed to stand for at least 2 to 3 days to separate into 2 or 3 layers, and then, when separated into 2 layers, the reddish brown aqueous solution of the middle layer is aged for 3 months or more (ageing). Clear and clear reddish brown and light reddish brown obtained by removing unstable components from wood vinegar by filtering the liquid or crude wood vinegar with a filter or by distillation such as after adsorption and filtration. And the pale yellow water-soluble liquid is called refined wood vinegar (Article 6, Notification No. 1999-35, Forestry Research Institute).

Wood vinegar contains more than 200 organic acids and more than 10 kinds of minerals and vitamins. It has excellent SOD (superoxide dismutase) function that removes harmful free radicals, removes foreign substances in cells and normalizes the respiratory circuit of cells. It is already widely used in foods and medicines as a substance that acts beneficially by keeping cells healthy.

With the recent increase in the consumption of livestock products, consumers' demands for the safety of livestock products have risen more than ever before, and countries around the world are fighting the quarantine war for animal disease prevention, a total knifeless war, to simultaneously protect their livestock producers and consumers. I'm working. Despite the development of state-of-the-art vaccines (prevention drugs) to prevent diseases, the incidence of diseases is increasing every year, and producers of livestock farmers use synthetic antimicrobial agents (chemotherapeutics) or antibiotics for the prevention and treatment of diseases. Although antibiotics are used, the problems caused by their overuse and misuse can lead to a slowing of the therapeutic effect due to the resistance of intestinal and pathogenic bacteria in livestock, and further the use of more antibiotics. have. Furthermore, the effects of these antimicrobial substances on eggs, milk and meat are transferred to and remain in public health, and when resistant bacteria enter the human intestine through livestock foods, they deliver resistance factors to the intestinal bacteria to treat human diseases. Problems are raised that are likely to slow down.

According to the data released by the Ministry of Agriculture and Forestry this year, the inconsistency rate due to disease identification or parasite inspection during the dismantling test during slaughter test is reported as 5.99% for Korean cattle, 2.33% for pigs and 0.39% for chickens. The proportion of inadequate disease caused by digestive diseases was 50.1% in Korean cattle, 26.0% in pigs, and 26.2% in chickens, which was relatively higher than respiratory diseases, circulatory diseases, and reproductive diseases.

Digestive diseases of animals are caused by pathogens such as bacteria, viruses, and parasites, and non-communicable factors such as environment, nutrition, and stress, alone or in combination, and are mainly accompanied by diarrhea. In recent years, intensive large-scale production, high-density breeding, continuous improvement of genetic production capacity of animals, changes in intestinal microflora due to overuse of drugs, deepening nutrient imbalances in feed ingredients, and chemicals for production and storage of raw grains Increasing use of treatments adds stress to animals, leading to atrophy of the digestive system and immune function, making them more resistant to disease.

Selye, when an animal is stressed, contracts the thymus and lymphatic organs, which decreases immune and biological defenses, increases digestive diseases such as indigestion or stomach or duodenal ulcers, Reported an increase in adrenality, and Meyer reported four times higher upper respiratory tract infections when stressed.

Specifically, Infectoius bursal disease, Marek's disease, Reticuloendotheliosis, Hypoglycemia, Actinobacillus pleuropneuomonia, Infectious chicken anemia, Reo virus disease, Fungus The increasing incidence and proliferation of immunodestructive diseases, such as toxin poisoning (Mycotoxicosis), leads to severely impaired immune function in animals, greatly increasing their susceptibility to disease.

Therefore, probiotics are widely used for improving immunity to animals and maintaining and improving the function of the digestive organs. Probiotics are living microbial agents that have a beneficial effect on the host animal by balancing and improving the intestinal microflora, increasing anti-microbial resistance against various diseases, reducing diarrhea, reducing serum cholesterol levels and phagocytosis by leukocytes. (Gerald W. Tannock, 1999, Probiotics a critical review, p1, Horison scientific press).

Isolauri suggests that probiotics affect phagocytosis along with anti-inflammatory cytokins that cause immunoglobulin-A (IgA) reactions when viruses or bacteria enter the animal's small intestine epithelium. It was. Kaila et al. Reported that diarrhea and diarrhea were increased by increasing the secretion of immunoglobulin A (IgA) in the intestine during diarrhea when ingesting lactic acid bacteria. Duffy et al. Rota virus diarrhea was relieved by the immune boosting effect when fed to animals.

However, because these probiotics differ from the test tube of animals, there is a problem in that the evidence indicating permanent settling in the intestine should be administered continuously if the evidence indicating permanent settling is unclear and a continuous effect is required (Fuller, 1992, Livestock Research Vol. 46, No. 46). 1, p44). In addition, the expected effect is insufficient depending on the environmental conditions and there is a problem in stability, so there is a problem in the quality of the distribution process or the processing of feed, especially in the case of feed such as pellets, the number of bacteria decreases during the heat treatment process. Effects such as non-uniformity of effects (In-Ho Lee, 2000, Technical Management Approach to Direct-fed Microbials Theory, p17-19, Shinwoo Plant Livestock Information Center).

Recently, in order to solve the problem of the stability of the probiotics described above, the culture of the bactericidal agent, tindal lactic acid bacteria, has been used for the purpose of enhancing the immunity and diarrhea prevention of livestock. Expensive livestock is not widely used in the situation.

The purpose of the present invention is to study the problem based on a wide range of basic investigations to solve the above problems, as a result of the formulation of ascorbic acid-treated stabilizing wood vinegar complex in the culture of tindal lactic acid bacteria, the existing cultures of tindal lactic acid bacteria when fed to animals It is to provide a composition for immunity boosting and dressing for animals that is superior to the formulation and has an excellent immune enhancing effect.

The present invention relates to an animal immunostimulating and formal composition comprising 2 to 30% by weight of ascorbic acid-treated stabilized wood vinegar and 2 to 20% by weight of tindal lactobacillus culture.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

Ascorbic acid-treated stabilizing wood vinegar used in the present invention (hereinafter referred to as stabilizing wood vinegar) is a stabilizing wood vinegar disclosed in the Republic of Korea Patent Application No. 102001-000000 of the wood vinegar prepared according to the conventional wood vinegar preparation method in the pre-aging step 1-20% by weight of ascorbic acid was added and dissolved, aged at room temperature for 6 months, and then obtained by filtration with a 200 (mesh) filter. Ascorbic acid was used to stabilize and activate wood vinegar, and it is possible to enhance the stability and efficacy of wood vinegar by adding 1-20% by weight of wood vinegar. This prevents the oxidation of wood vinegar by inactivating oxygen oxidation ability by ascorbic acid in the electron transport system, and ascorbic acid combines with heavy metals remaining in wood vinegar to improve the purity of wood vinegar. It seems to be because it affects.

The ascorbic acid-treated stabilizing wood vinegar is preferably used 2 to 30% by weight, less than 2% by weight of the immune boosting and diarrhea prevention effect is insufficient, when more than 30% by weight is excellent but the composition stability There is a disadvantage that this is lowered and inconvenience in use. That is, the stabilized wood vinegar used in the present invention is in a liquid phase, and thus, when the content of the stabilized wood vinegar is increased, the content of the stabilized wood vinegar should be used by adsorbing it to 100 to 200 mesh zeolites in the preparation of animal immunostimulation and formal dressings. This increases the risk of product deterioration, and when added to the feed it takes a lot of mixing time.

The culture of tindal lactic acid bacteria used in the present invention is finally obtained by repeating the lactic acid bacteria strain contained in the GRAS (Generally Regarded As Safe) microorganism listed in the FDA several times during the fermentation process. As a fungicide culture, it has been proved to have an excellent intestinal action and a slight local immune boosting effect, and is widely used in humans and animals as a diarrhea prevention and treatment agent. The bactericidal agent produced by the tindal heat treatment method includes a heat-resistant binding factor that helps to strongly adhere to the receptor site of the intestinal epithelial cell, which is responsible for blocking the adhesion of the pathogens of the pathogen and discharging it into the body. It contains immune enhancing factors that activate gut topical immunity, bacteriostatic substances produced during the process of tindalization, and lactic acid bacteria proliferative substances such as organic acids and vitamin B groups to prevent intestinal digestive diseases such as diarrhea and stool. This fungal culture prevents diarrhea by blocking and eviction, which prevents pathogens from attaching to the intestinal mucosa and excreting it in vitro, and competitive exclusion principles that prevent pathogens from working in the intestine by attaching a much stronger mesenteric membrane than the pathogen. As a new concept diarrhea preventive agent that is completely different from existing antibiotics and probiotics, it is not resistant and stable to heat and acid, so that it can be used safely by feed mills and animal breeders (Hyundai Swine No. 153, p117-119, Hyun-Sung Co., Ltd.).

The content of the tindal lactic acid bacteria culture is preferably 2 to 20% by weight, but less than 2% by weight of the effect of immunopromoting and diarrhea prevention is insufficient, in the case of more than 20% by weight with stabilized wood vinegar Production of the composition itself to maintain the proper ratio is difficult and uneconomical problems are undesirable.

It is preferable to use the tydallized Lactobacillus acidophilus culture (hereinafter, referred to as TLAC) as the chintalized lactic acid bacteria culture.

It is recommended that the composition of the present invention is added to the animal feed, mixed and administered, and mixed and administered to the animal feed at a rate of 0.04 to 0.3%. The rate of addition to the feed can vary depending on the growth stage, health and disease state of the animal.

The present invention can be understood in more detail by the following examples and comparative examples, the following examples are only for illustrating the present invention and are not intended to limit the protection scope of the present invention.

Examples and Comparative Examples

<Example 1>

Stabilized wood vinegar treated with 5% by weight of ascorbic acid described in Korean Patent Application No. 10-2001-0050219 was adsorbed to zeolite (Kangje Zeolite, Wangje Chemical) at a ratio of 1: 4 (stabilized wood vinegar: zeolite) and dried. 40 g of stabilized wood vinegar powder (1% by weight as stabilized wood vinegar) and 10% by weight of TLAC (ZOOLOC concentrated, manufactured by Lacteol, France) were mixed for 5 minutes, and then calcium carbonate was added to 100% by weight and mixed for 5 minutes. Animal immunostimulation and formal preparations were prepared.

<Example 2>

Except for adding 5% by weight of stabilized wood vinegar in the same manner as in Example 1 was prepared for animal immunostimulation and formal preparation.

<Example 3>

Except that 10% by weight of stabilized wood vinegar was prepared in the same manner as in Example 1 to prepare an animal immune booster and formal preparation.

<Example 4>

Except for adding 20% by weight of stabilized wood vinegar in the same manner as in Example 1 was prepared for animal immunostimulation and formal preparation.

Comparative Example 1

Calcium carbonate was added to 10% by weight of TLAC to 100% by weight, and mixed for 7 minutes to prepare an animal immune booster and suit.

Experimental Example 1

Comparative test for the prevention of diarrhea on piglets

1. Experiment Method

The mothers weaned the weaning piglets into 24 groups of 24 and divided them into five groups, and fed 14, 1, 2, 3, 4 and Comparative Example 1 to the weaning pig feed (powdered feed) produced by the Agricultural Cooperatives for 14 days, respectively. The number of diarrhea and the number of coliform bacteria in the feces were measured up to 1 week later. E. coli counts for each group were aseptically collected 1 gram of fecal excreted by each pig in each group, diluted with diluent and incubated for 48 hours at 37 ℃ using a DHL medium (Youngyeon Chemical Co., Ltd.) Measured.

2. Experimental Results

Comparative Example 1 group showed diarrhea incidence with 2 heads of diarrhea and 8.3%, and Example 1 feed group containing 1% by weight of stabilized wood vinegar also did not improve as 2 heads of diarrhea occurred. It was. However, Example 2 feed group containing 5% by weight of stabilized wood vinegar was 4.2% diarrhea incidence, diarrhea incidence of Example 3, 4 pay group was proved to significantly improve the effectiveness of diarrhea prevention when combined prescription of stabilized wood vinegar solution .

In fecal coliforms, 91.1% of the control group of the comparative example 1 was decreased on the 14th day after the end of the salary, and increased rapidly again on the 7th day after the end of the salary. In the pig meal fed Example 1, 93.3% of the salary was reduced on the 14th day and 58% of the end of the salary was reduced, compared to that of Comparative Example 1, but the effect of reducing E. coli was not satisfactory. However, E. coli counts were reduced by 98.2%, 99.1%, and 99.4%, respectively, on day 14 after salary, and 96.5%, 97.1%, and 98.5 on day 7, respectively. The percentage was reduced and proved to be excellent suiting effect. The results are shown in Tables 1 and 2 below.

Diarrhea Incidence and Incidence Rate Example 1 salary group Example 2 salary group Example 3 pay group Example 4 Salary Group Comparative Example 1 salary group Trials 24 heads 24 heads 24 heads 24 heads 24 heads Diarrhea 2 1 head 0 heads 0 heads 2 heads Diarrhea Rate 8.3% 4.2% 0% 0% 8.3%

Average value of E. coli flora in each group 1 day before administration Day 7 Dosing Day 14 Dosing 7 days after suspension Example 1 Salary Group 1.6 × 10 ⁵ 5.6 × 10 ⁴ 1.1 × 10 ⁴ 6.7 × 10 ⁴ Example 2 Salary Group 2.3 × 10 ⁵ 6.1 × 10 ⁴ 4.1 × 10 ³ 8.1 × 10 ³ Example 3 Salary Group 1.8 × 10 ⁵ 2.7 × 10 ⁴ 1.6 × 10 ³ 5.2 × 10 ³ Example 4 Salary Group 2.1 × 10 ⁵ 2.5 × 10 ⁴ 1.2 × 10 ³ 3.1 × 10 ³ Comparative Example 1 Salary Group 1.0 × 10 ⁵ 3.9 × 10 ⁴ 8.9 × 10 ³ 4.6 × 10 ⁴

Experimental Example 2 Humoral Immune Increasing Effect of Chicken

1. Experiment Method

1) Experimental Animal

This experiment was carried out by dividing 75 number of brown laying chicks into 15 groups, each of 15 groups including Example 1, 2, 3, 4 and Comparative Example 1 salary group.

2) Experiment Method

① Administration method: All chicks were inoculated with Newcastle disease virus B1 live poison vaccine at 28 days of age by 106EID50 of allowance, and each of the groups was treated with compositions of Examples 1, 2, 3 and Comparative Example 1 for 14 days from 21 days to 35 days of age. 60 mg daily.

② Measurement of HI antibody: 2 ~ 3cc of blood was collected from subcutaneous vein at 42 days of age, 2 weeks after vaccination, and serum was separated and diluted to 212 using microplate plate. Antibody titer was measured.

2. Experimental Results

In order to investigate the humoral immune-promoting effect of experimental animals, HI antibody titer of Newcastle virus B1 was analyzed two weeks after vaccination, and the comparative group 1 was 58.49 and the administration of Example 1 was also 58.49. However, in Example 2, the pay group was 73.52, which was 25.7% higher than that of Comparative Example 1, and in Examples 3 and 4, respectively, 106.15 and 111.43 were increased by 81.5% and 90.5%, respectively, compared to Comparative Example 1. . The results are shown in Table 3 below.

HI Antibody Titer of Newcastle Virus B1 division Test head lig ₂ titer distribution Average Decimal Correction One 2 3 4 5 6 7 8 9 10 11 12 Example 1 Salary Group 15 - - - 2 4 4 4 One - - - - 5.87 58.49 Example 2 Salary 15 - - - - 4 6 3 2 - - - - 6.20 73.52 Example 3 Salary 15 - - - - 2 5 4 3 One - - - 6.73 106.15 Example 4 Salary Group 15 - - - One - 5 4 5 - - - - 6.80 111.43 Comparative Example 1 salary group 15 - - - One 5 5 3 One - - - - 5.87 58.49

As can be seen from the above, the animal immunosuppression and formal dressing composition prepared by the present invention is superior to the immune boosting and formal effect by applying stabilizing wood vinegar to the culture of tindal lactobacillus culture alone. A synergistic effect can be obtained.

Claims (3)

1 to 20% by weight of ascorbic acid is added to a conventional wood vinegar, dissolved, aged at room temperature for 6 months, and then filtered using a filter. Animal immunostimulating and formal composition for. According to claim 1, Ascorbic acid-treated stabilizing wood vinegar is 2 to 30% by weight, chitin and lactic acid bacteria culture is characterized in that 2-20% by weight of the composition for animal immunostimulation and formal use. According to claim 1 or 2, wherein the chitin is lactic acid bacteria culture animal immunostimulating and formal composition, characterized in that the chitin is Lactobacillus asidophilus culture.