KR102722620B1 - Manufacturing method of animal feed additives - Google Patents

Abstract

The present invention discloses a method for manufacturing a pet feed additive, comprising: a step of culturing a composite strain culture by inoculating a medium with lactic acid bacteria and Bacillus subtilis; a step of separating the composite strain culture into a composite strain and a culture supernatant, and then separately selecting the culture supernatant; a step of dissolving an inorganic mineral in the culture supernatant to form an inorganic mineral solution; a step of dissolving an amino acid in the culture supernatant to form an amino acid solution; a step of mixing the inorganic mineral solution and the amino acid solution to form an organic mineral solution through a chelating reaction; and a step of freeze-drying the organic mineral solution.

Description

{Manufacturing method of animal feed additives}

The present invention relates to a method for producing a pet feed additive, comprising: a step of culturing a composite strain culture by inoculating a medium with lactic acid bacteria and Bacillus subtilis; a step of separating the composite strain culture into a composite strain and a culture supernatant, and then separately selecting the culture supernatant; a step of dissolving an inorganic mineral in the culture supernatant to form an inorganic mineral solution; a step of dissolving an amino acid in the culture supernatant to form an amino acid solution; a step of mixing the inorganic mineral solution and the amino acid solution to form an organic mineral solution through a chelating reaction; and a step of freeze-drying the organic mineral solution.

As modern society continues to experience changes in its population structure, such as the acceleration of aging and the increase in single-person households, the number of people who perceive their pets as family members or life companions rather than simply pets is increasing.

Pets live with people and are protected as family members, but they are also exposed to polluted natural environments such as air and water pollution, processed foods, and stress that people experience, so they are vulnerable to various diseases such as skin diseases, respiratory diseases, and digestive disorders that are common to people. In addition, pets mainly live indoors, so they do not get much exercise, which raises concerns about obesity and increases the possibility of secondary diseases.

In the past, people's perception of pet health was such that when pets showed symptoms of various diseases, they were mostly treated by taking them to a veterinary hospital for treatment or prescription medication, and passive care was sufficient.

However, recently, the guardians' thoughts on the health of their pets are focusing on ways to prepare for various diseases and infections in advance by improving the fundamental immune system, similar to human health management.

Accordingly, in order for companion animals to live healthier lives, active development of technologies for various pet foods, nutritional supplements, and medicines is underway, and the domestic pet-related market size is showing a rapid growth trend every year. For example, essential nutrients in pet food compositions are evenly mixed, or high-protein or vitamins and minerals are mixed in high amounts to supplement specific nutrients or physiologically active ingredients, and in addition to feed, nutritional supplements for companion animals are released to enhance immunity. Or, programs such as feeding systems for companion animals that can effectively feed appropriate amounts of feed and nutritional supplements are developed.

Antibiotics, which have been used as a representative example of such feed additives, have been continuously raising issues regarding their overuse, and in particular, developed countries such as the UK and the US have established committees to deal with such issues, and the EU and WHO have also been strictly prohibiting the use of antibiotics, antimicrobials, growth promoters (AMGPs) and hormones used in humans since 1997.

Accordingly, there is a growing demand for the development of new products to replace antibiotics or probiotics for feed additives in Korea, and interest in enzymes, probiotics, and plant-based bioactive substances as alternatives to antibiotics is increasing.

However, in the case of domestic antibiotic substitutes and feed additives, most are simple liquid probiotics, and research and development is focused on finding microorganisms rather than formulations.

Meanwhile, pet food generally contains various nutrients, and representative minerals include zinc (Zn), chromium (Cr), magnesium (Mg), and iron (Fe).

Although the above minerals exist in small proportions in animal tissues and act as heavy metals that cause poisoning when present in excessive amounts, they are very important because they act as essential trace minerals that are absolutely necessary in the body.

However, if the above minerals are inorganic, their absorption rate in the body is very low, so if the amount used is very high, it can cause poisoning symptoms in animals, and minerals that are not absorbed can be excreted from the body and become a source of soil and water pollution.

In addition, since the bioavailability of inorganic minerals included in feed additives is only 20-30% or less, and the remainder is released as manure, developed countries are strengthening regulations on the use of such inorganic minerals, and Korea is also restricting them. In particular, as of March 25, 2020, the compost maturity requirement has been implemented, and the copper and zinc contents in compost must be managed below 500 ppm and 1,200 ppm, respectively, so the management of inorganic minerals in feed is urgent.

Republic of Korea Patent Publication No. 10-1929492 (Animal feed additive and its manufacturing method)

The present invention was invented to solve the above problems, and the purpose of the present invention is to provide a method for manufacturing a pet feed additive that can increase the growth rate by increasing the absorption rate in the body, can expect an effect of improving immune function, and can reduce the amount of minerals excreted from the body.

For the above purpose, the present invention comprises a medium comprising 94 to 98 wt% of purified water, 1 to 3 wt% of glucose, 0.5 to 2 wt% of yeast extract, 0.1 to 0.5 wt% of sodium chloride (NaCl), and 0.1 to 0.5 wt% of potassium hydrogen phosphate ( _K2HPO4 ), which is sterilized at 120 to 125°C for 15 minutes, and then lactic acid bacteria and Bacillus subtilis are inoculated into the medium, and then cultured _for 24 to 36 hours at a temperature of 30 to 37°C, an air injection amount of 0.2 to 0.4 vvm, and a rotation speed of 50 to 200 rpm, and one or more of a seawater-derived mineral concentrate, an aqueous sulfur dispersion, or an aqueous illite dispersion is added to the medium so as to promote the growth of the lactic acid bacteria and Bacillus subtilis, thereby culturing a complex strain culture. It is characterized by including a culturing step; a culture supernatant preparation step of separating the composite strain culture into a composite strain and a culture supernatant, and then separately selecting the culture supernatant; an inorganic mineral solution composition step of dissolving inorganic minerals in the culture supernatant to create an inorganic mineral solution; an amino acid solution composition step of dissolving amino acids in the culture supernatant to create an amino acid solution; an organic mineral solution composition step of mixing the inorganic mineral solution and the amino acid solution through a chelating reaction to create an organic mineral solution; and a freeze-drying step of freeze-drying the organic mineral solution.
In addition, the present invention comprises a medium comprising 30 to 50 wt% of glucose, 20 to 40 wt% of yeast culture, 25 to 30 wt% of corn starch, and 1 to 5 wt% of glutamic acid, sterilizing the medium at 120 to 125°C for 15 minutes, inoculating the medium with lactic acid bacteria and Bacillus subtilis, and culturing the medium at a temperature of 30 to 37°C, an air injection amount of 0.2 to 0.4 vvm, and a rotation speed of 50 to 200 rpm for 24 to 36 hours, wherein one or more of a seawater-derived mineral concentrate, an aqueous sulfur dispersion, or an aqueous illite dispersion is added to the medium so as to promote the growth of the lactic acid bacteria and Bacillus subtilis, thereby culturing the complex strain culture, the complex strain culture culturing step; a culture supernatant preparation step of separating the complex strain culture into a complex strain and a culture supernatant, and then separately selecting the culture supernatant; It is characterized by including an inorganic mineral solution composition step of dissolving inorganic minerals in the culture supernatant to create an inorganic mineral solution; an amino acid solution composition step of dissolving amino acids in the culture supernatant to create an amino acid solution; an organic mineral solution composition step of mixing the inorganic mineral solution and the amino acid solution to create an organic mineral solution through a chelating reaction; and a freeze-drying step of freeze-drying the organic mineral solution.

delete

In addition, in the present invention, the step (S30) of forming an inorganic mineral solution is characterized in that the step comprises forming an inorganic mineral solution by dissolving 1 to 10 parts by weight of any one inorganic mineral selected from the group consisting of chromium (Cr), zinc (Zn), iron (Fe), copper (Cu), selenium (Se), manganese (Mn), boron (B), titanium (Ti), germanium (Ge), and calcium (Ca) with respect to 100 parts by weight of the culture supernatant, and an organic acid is further added to adjust the pH of the culture supernatant to promote the dissolution of the inorganic mineral.

In addition, in the amino acid solution composition step of the present invention, the amino acid is glycine, and 0.1 to 10 parts by weight of glycine is added per 100 parts by weight of the culture supernatant, and glutamic acid is further added to promote a chelating reaction with the inorganic mineral solution.

In addition, in the step of forming the amino acid solution according to the present invention, the amino acid is glycine, and 0.1 to 10 parts by weight of the glycine is added based on 100 parts by weight of the culture supernatant, and glutamic acid is further added to promote a chelating reaction with the inorganic mineral solution.

In addition, the present invention is characterized by including a step of mixing and stirring the organic mineral powder manufactured in the freeze-drying step, water, excipients, preservatives, and functional additives.

The pet feed additive manufactured according to a preferred embodiment of the present invention, which is made as described above, can increase the growth rate by improving the absorption rate of minerals in the body through the chelate bond of minerals and amino acids, can expect the effect of improving immune function, and has a remarkable effect of reducing the amount of minerals excreted from the body.

Figure 1 is a manufacturing process diagram of a pet feed additive according to the present invention.

Hereinafter, a method for manufacturing a pet feed additive according to the present invention will be described in more detail with reference to the attached drawings.

The present invention discloses a method for manufacturing a pet feed additive that can increase the growth rate by improving the absorption rate of minerals in the body through a chelate bond between minerals and amino acids, can expect an effect of improving immune function, and can reduce the amount of minerals excreted from the body.

FIG. 1 is a manufacturing process diagram of a pet feed additive according to a preferred embodiment of the present invention. As shown in FIG. 1, the present invention includes a step of culturing a complex strain culture solution (S10); a step of preparing a culture supernatant (S20); a step of forming an inorganic mineral solution (S30); a step of forming an amino acid solution (S40); a step of forming an organic mineral solution (S50); and a step of freeze-drying (S60).

First, the complex strain culture solution culturing step (10) is a step of culturing the complex strain culture solution by inoculating lactic acid bacteria and Bacillus subtilis into the medium, and the culture supernatant preparation step (S20) is a step of separating the complex strain culture solution into the complex strain and the culture supernatant, and then selecting the culture supernatant separately.

The above medium can be used as an appropriate medium to facilitate the cultivation of lactic acid bacteria and Bacillus subtilis.

As an example, the medium may be a medium composed of purified water, glucose, yeast extract, sodium chloride (NaCl), and potassium hydrogen phosphate (K ₂ HPO ₄ ). Preferably, the medium may be composed of 94 to 98 wt% of purified water, 1 to 3 wt% of glucose, 0.5 to 2 wt% of yeast extract, 0.1 to 0.5 wt% of sodium chloride (NaCl), and 0.1 to 0.5 wt% of potassium hydrogen phosphate (K ₂ HPO ₄ ). Here, when the glucose is less than 1 wt% with respect to the total weight of the medium composition, enzyme activity is not induced, and when it exceeds 3 wt%, enzyme activity does not increase further, which is inefficient. If the yeast extract is less than 0.5 wt% based on the total weight of the medium composition, the strain cannot grow smoothly, and if it exceeds 2 wt%, it is inefficient because it does not have a significant effect on the strain growth amount even if it is not used more than that. In addition, the potassium hydrogen phosphate (K ₂ HPO ₄ ) is a culture promoter, and if it is less than 0.1 wt% based on the total weight of the medium composition, the number of lactic acid bacteria may decrease, which may lower the yield.

In addition, according to another embodiment of the present invention, the medium may contain glucose, yeast culture, corn starch, and glutamic acid. Preferably, the medium may contain 30 to 50 wt% of glucose, 20 to 40 wt% of yeast culture, 25 to 30 wt% of corn starch, and 1 to 5 wt% of glutamic acid. Glucose is completely decomposed in about 3 days, leaving no residual sugar, thereby providing nutrients necessary for the medium while maintaining the purity of the cultured mycelia.

Additionally, the medium may include yeast extract, anhydrous glucose, soy peptone, taurine, and minerals, or may include anhydrous glucose, yeast extract, sodium acetate, and dibasic potassium phosphate (K ₂ HPO ₄ ), or may include yeast extract, yeast culture, glucose, sodium acetate, and citric acid, or may include yeast extract, sugar, and glucose.

The above lactic acid bacteria, after entering the intestines, attach to intestinal epithelial cells, thereby preventing the colonization of harmful microorganisms in the intestines and secreting antibacterial substances, thereby suppressing the growth of harmful microorganisms and improving diarrhea and constipation, as well as enhancing immune activity, having anticancer effects, and lowering serum cholesterol.

In the present invention, the lactic acid bacteria inoculated into the medium are Lactobacillus bulgaricus , Lactobacillus acidophilus , Lactobacillus delbrueckii , Lactobacillus plantarum , Lactobacillus rhamnosus , Lactobacillus delbrueckii subsp. lactis, Latobacillus lactis, Lactobacillus reuteri , Lactobacillus brevis , Lactobacillus salivarius , Lactobacillus casei, Lactobacillus It can be one selected from Lactobacillus curvatus, Lactobacillus crispatus , Lactobacillus paracasei , Lactobacillus fermentum, Lactobacillus perolens, or Lactobacillus helveticus .

The above-mentioned Bacillus subtilis increases feed utilization by producing protein and starch decomposition enzymes, and has strong heat resistance due to its medium and high temperature. It improves feed efficiency by promoting the absorption of nutrients and producing organic products, and relieves stress, enhances immunity, and promotes growth. In addition, Bacillus subtilis decomposes a large number of carbohydrates to produce acid, and secretes a large amount of protein into the medium.

In the present invention, the Bacillus subtilis inoculated into the medium may be one selected from Bacillus amyloliquefaciens , Bacillus macerans, Bacillus mojavensis , Bacillus pumilus , Bacillus vallismortis , Bacillus velezensis , Bacillus lentus , Bacillus licheniformis , Bacillus cereus , Bacillus coagulans, or Bacillus polyfermenticus .

When culturing the above complex strain culture, it is preferable to first sterilize the medium at 120 to 125°C for 15 minutes, then inoculate the medium with lactic acid bacteria and Bacillus subtilis, and then culture for 24 to 36 hours at a temperature of 30 to 37°C, an air injection amount of 0.2 to 0.4 vvm, and a rotation speed of 50 to 200 rpm.

Here, a seawater-derived mineral concentrate, an aqueous sulfur dispersion or an aqueous illite dispersion can be added to the medium to promote the growth of the lactic acid bacteria and Bacillus subtilis.

Concentrated mineral water derived from seawater refers to concentrated mineral water with a salinity of 30-40% with increased mineral content by concentrating seawater or brine using solar salt, or by mixing concentrated seawater and concentrated sea salt.

Seawater usually has a salinity of 33 to 37% and contains various minerals, including nutrients such as nitrogen, phosphorus, and silicon necessary for plant growth. However, since seawater contains an excess of sodium, the sodium can be removed to concentrate the seawater and used. The resulting seawater-derived mineral concentrate has a significantly reduced salt content and is a liquid mineral mixed with a high content of minerals useful for companion animals, such as magnesium, potassium, and calcium. Aqueous solutions containing minerals such as magnesium, potassium, and calcium have low solubility in water, and therefore, only a small amount is absorbed when consumed by companion animals, which reduces the efficiency of use. However, the above seawater-derived mineral concentrate does not have minerals precipitated but exists in an excessively dissolved state, so companion animals can easily absorb it, thereby increasing the efficiency of use of the minerals.

Preferably, the seawater used is deep ocean water. Deep ocean water is seawater that generally exists in the ocean at a depth of 200 m or less, and since there is no phytoplankton that consumes nutrients and photosynthesis does not occur in the deep sea where sunlight does not reach, it is rich in inorganic nutrients such as nitrogen, phosphorus, and silica, and since it remains stagnant in a deep place for a long time, various minerals such as magnesium, calcium, potassium, and sodium and trace elements such as zinc, selenium, and manganese exist in a stable form in large quantities, and there is no pollution by the atmosphere or inflowing water, and the number of living bacteria is not high compared to surface water, and general bacteria are not contaminated, so it has superior cleanliness compared to surface water or groundwater.

The above sulfur aqueous dispersion refers to an aqueous solution containing 10 to 20 wt% of sulfur powder. Sulfur is an essential element that constitutes a living organism, and in particular, it affects the production of amino acids and is necessary for protein synthesis, and thus is effective in improving growth and development and meat quality, and strengthens immunity. However, since inorganic sulfur or synthetic organic sulfur of mineral substances exhibits toxicity and strong acidity, legal sulfur can be used.

The above-mentioned aqueous dispersion of illite refers to an aqueous solution containing 10 to 20 wt% of illite powder. Illite is a non-swelling mineral that is formed when potassium, magnesium, etc. are dissolved during the weathering of mica clay minerals. The silicon dioxide (SiO ₂ ) component, which accounts for 60 to 70% of the composition, solves problems such as oversupply of mineral components, and together with the mineral components derived from seawater, increases the survival of lactic acid bacteria.

In the present invention, one or more of the above-mentioned seawater-derived mineral concentrate, sulfur aqueous dispersion, or illite aqueous dispersion may be added, and it is preferable that the total amount is added in an amount of 1 to 10 parts by weight per 100 parts by weight of the medium. Preferably, all three types are added for the above-mentioned various effects.

The method for separating the above culture solution into strain and culture supernatant can utilize various known methods, for example, the culture solution can be placed in a centrifuge to separate the strain and culture supernatant. The above culture supernatant is a metabolic product produced during the fermentation culture process of lactic acid bacteria and Bacillus subtilis, and can provide various functional effects by the above-mentioned lactic acid bacteria and Bacillus subtilis.

Next, the step of forming the inorganic mineral solution (S20) is a step of forming the inorganic mineral solution by dissolving inorganic minerals in the culture supernatant.

In the present invention, the inorganic mineral may be any one mineral selected from the group consisting of chromium (Cr), zinc (Zn), iron (Fe), copper (Cu), selenium (Se), manganese (Mn), boron (B), titanium (Ti), germanium (Ge), and calcium (Ca).

An adequate supply of minerals such as chromium (Cr), zinc (Zn), iron (Fe), and copper (Cu) is essential for the growth of companion animals. For example, zinc (Zn) is a component of the enzymes involved in growth, so when it is deficient, it can lead to decreased feed intake and feed efficiency, reproductive organ developmental delay, pregnancy and lactation disorders, abnormal skeletal formation, and weakened resistance to skin pathogens. In addition, iron (Fe) is a component of heme, which forms hemoglobin, which transports oxygen in the body. When it is deficient, hemoglobin synthesis decreases, lowering the energy metabolism rate and causing anemia. In addition, selenium (Se) is a trace mineral essential for various antioxidant, anti-inflammatory, and antiviral effects, and as an antioxidant, it is known to remove active oxygen such as hydrogen peroxide that causes cell membrane damage, prevent or delay the aging and degeneration of tissues, and help strengthen immunity and improve blood circulation. That is, although these minerals occupy a small proportion in animal tissues, they play a very diverse and important role in forming the skeleton, regulating the osmotic pressure in the body, maintaining the acid-base balance of body fluids, and acting as activators in enzyme systems or as components of the enzymes themselves.

According to an embodiment of the present invention, the inorganic mineral solution may be prepared by dissolving 1 to 10 parts by weight of the inorganic mineral per 100 parts by weight of the culture supernatant. If the inorganic mineral is dissolved in less than 1 part by weight, the expected effect may not be obtained due to insufficient content. In addition, if the mineral is in an inorganic form, the absorption rate in the body is significantly reduced, and unabsorbed minerals are excreted from the body and become a source of soil and water pollution, so it is preferable that it does not exceed 10 parts by weight.

In addition, in the step (S20) of forming the inorganic mineral solution according to an embodiment of the present invention, an organic acid may be further added to adjust the pH of the culture supernatant when dissolving the inorganic mineral in the culture supernatant to promote the dissolution of the inorganic mineral.

Organic acid is a general term for organic compounds that are acidic, and organic compounds containing carboxyl groups and sulfonic groups are representative organic acids. The organic acid can be one selected from acetic acid, butyric acid, palmitic acid, oxalic acid, and tartaric acid.

The above organic acid can be used in a liquid form by dissolving it in distilled water.

The organic acid may be added in an amount of 0.5 to 0.7 parts by weight per 100 parts by weight of the culture supernatant. For example, in the process of dissolving the inorganic mineral, 0.5 to 0.7 parts by weight of acetic acid may be additionally added per 100 parts by weight of the culture supernatant in the process of dissolving the inorganic mineral after adding the inorganic mineral to the culture supernatant. This may enable the dissolution of the inorganic mineral to occur more smoothly.

Next, the amino acid solution composition step (S30) is a step of dissolving amino acids in the culture supernatant to create an amino acid solution.

According to one embodiment of the present invention, the amino acid solution can be prepared by dissolving 0.1 to 10 parts by weight of amino acid per 100 parts by weight of the culture supernatant.

Here, the amino acid can be one selected from the group consisting of lysine, arginine, histidine, cysteine, phenylalanine, tyrosine, methionine, and glycine.

Preferably, the amino acid may be glycine, and the amino acid solution composition step may be performed by dissolving 0.1 to 10 parts by weight of glycine per 100 parts by weight of the culture supernatant to form an amino acid solution.

Additionally, according to an embodiment of the present invention, glutamic acid may be further added in the amino acid solution composition step to assist in the chelation of glycine.

Glutamic acid is an activator that activates the amino group of glycine, and forms an intermediate that can react with the amino group, facilitating the bonding reaction. That is, by adding glutamic acid, the activation of the amino group is enhanced, and the efficiency of the organic passivation bonding reaction is increased.

The above glutamic acid may be added in an amount of 0.1 to 10 parts by weight per 100 parts by weight of the culture supernatant, and preferably may be added in a weight ratio of 1:1 with the glycine.

Meanwhile, when phenylalanine is selected as an amino acid, diisopropylcarbodiimide (DIC) can be added as an activator, and when methionine is selected, diisopropylcarbodiimide (DIC) or HATU (Hexafluorophosphate Azabenzotriazole Tetramethyl Uronium) can be added.

Next, the organic mineral solution composition step (S40) is a step of mixing the inorganic mineral solution and the amino acid solution to form an organic mineral solution through a chelating reaction. At this time, the inorganic mineral solution and the amino acid solution can be mixed at a weight ratio of 1:1.

Chelation refers to a bond in which a mineral is held by a ligand as if with claws, and a chelate chemical bond refers to a state in which a chelating agent, an organic molecule with special properties, surrounds a metal element and forms a heterocyclic structural bond as if it were holding it tightly with tweezers on both sides.

When amino acids are chelated with inorganic minerals and converted into organic trace elements, the mineral absorption rate of companion animals is rapidly improved due to the binding action of surface ligands, and there are effects such as improved growth rate, milk production, reproductive efficiency, weight improvement, and immune function. In addition, as the digestion and absorption rate of minerals increases, the generation of fecal by-products can be reduced, and by suppressing substances causing odors generated in the intestines during digestion, air and water pollution can be prevented, and excessive mineral accumulation in the soil can be prevented.

According to a recently published research report, organic chromium lowers blood sugar, cholesterol and body fat, and strengthens muscle (GW Evans, Int J Biosocial Med Research 11(2), 163, 1989), and it is known that adding 200 ppb of organic chromium to pig feed not only lowers the fat and cholesterol levels of pigs but also increases muscle synthesis (K Jacques et al., Feed Management, 44, 23, 1993). Conventional feed additives contain minerals (e.g., zinc) in inorganic form, but these inorganic minerals have a digestibility of less than 20% and more than 80% are discharged in the form of manure, causing serious soil environmental pollution. The present invention has a remarkable effect in solving such problems.

Next, the freeze-drying step (S50) is a step of powderizing the organic mineral solution by freeze-drying it.

Freeze-drying is a process for drying the moisture contained in the main and auxiliary materials. The semi-finished animal feed additives manufactured through the above steps contain relatively little moisture, so they do not spoil easily and can secure a long shelf life. In addition, since moisture can be added to suit the pet's preference, it can satisfy the various preferences of the pet.

For example, the freeze-drying step can be performed at -40 to -50°C for 18 to 36 hours in a freeze dryer. If it is performed below the temperature and time range, the freeze-drying may not be performed smoothly, and if it is performed excessively above the temperature and time range at which the freeze-drying can be completed has already been exceeded, which may be uneconomical.

The pet feed additive of the present invention, prepared by this process, contains lactic acid bacteria and Bacillus subtilis components at 1.0x10 ⁶ cfu/g to 3.0x10 ¹² cfu/g, and thus can be expected to have effects such as enhanced immune activity, anticancer effects, stress relief, enhanced immunity, and growth promotion.

Example 1.

A medium composed of 96 wt% purified water, 2 wt% glucose, 1 wt% yeast extract, 0.5 wt% sodium chloride (NaCl), and 0.5 wt% potassium hydrogen phosphate (K ₂ HPO ₄ ) was prepared.

Next, Lactobacillus plantarum and Bacillus amyloliquefaciencs were inoculated onto the medium, and then the medium was cultured at 32°C, an air injection amount of 0.3 vvm, and a rotation speed of 100 rpm for 36 hours to simultaneously culture anaerobic Lactobacillus plantarum and aerobic Bacillus amyloliquefaciencs, thereby culturing a composite strain culture having a Lactobacillus plantarum content of 2.5 x 10 ⁸ cfu/g and a Bacillus amyloliquefaciencs content of 2.7 x 10 ⁹ cfu/g. Thereafter, the composite strain culture was separated into strains and culture supernatant through centrifugation.

Next, 3 parts by weight of zinc was added to 100 parts by weight of the culture supernatant and then mixed to form an inorganic mineral solution.

Next, 2 parts by weight of glycine was added to 100 parts by weight of the culture supernatant, and then 2 parts by weight of glutamic acid was further added to promote the organic passivation reaction of the glycine, and then mixed to form an amino acid solution.

Next, the inorganic mineral solution and the amino acid solution were mixed in a weight ratio of 1:1 to prepare an organic mineral solution, and then the prepared organic mineral solution was placed in a freeze dryer and freeze-dried at -50°C for 24 hours to prepare a pet feed additive (organo-passivated zinc powder with glycine) according to an embodiment of the present invention.

Next, the present invention may include a molding step (S60) of mixing and stirring the organic mineral powder prepared by freeze-drying the organic mineral solution, water, excipients, preservatives, and functional additives to shape the product.

First, the organic mineral powder may be added in an amount of 25 to 100 mg per 1 L of water. Here, if two or more types of the organic mineral powder are prepared, it is preferable that each organic mineral powder be added in an amount of 25 mg or more, and the total amount should not exceed 300 mg.

An excipient is a type of carrier used to form a composition containing an active ingredient into a product form. It is added to provide an appropriate hardness or shape, or to make it into a size that is easy to handle by providing a certain capacity or weight depending on the amount of the active ingredient added, which is the main ingredient.

In the present invention, the excipient is added to coagulate the organic mineral powder together with water to form the feed additive into a solid or semi-solid form (e.g., a gel). For example, the solid excipient may be one selected from bentonite and sodium gluconate, and the semi-solid excipient may be one selected from agar powder, collagen powder, fish collagen powder, and gelatin powder. The organic mineral powder has the advantage of being advantageous not only in a gel-type semi-solid form, but also in solid-type product forming and forming because it has many pores and can hold a large amount of moisture.

The above excipients can be added in an amount of 100 to 200 g per 1 L of water.

According to one embodiment of the present invention, agar powder may be added as the excipient. Agar is manufactured by freeze-drying an adhesive extracted from seaweed such as seaweed using seaweed as a raw material, and most of the ingredients are low-calorie dietary fiber. Dietary fiber has the effect of lowering cholesterol or high blood pressure. In addition to food, it is also used as an agar medium used in plant tissue culture or microbial culture. Since agar does not dissolve in water, it can maintain the organic mineral powder in a gel form.

In addition, the preservative is intended to prevent the growth or decay of microorganisms, and any ingredient that can be used in pet feed additives is not particularly limited and can be used. For example, the preservative can be sodium benzoate, sodium acetate, sodium lauryl sulfate, etc.

In addition, in the present invention, the functional additive may be added not only to promote the health of the companion animal, but also to improve the texture or flavor according to preference. For example, as the functional additive, one or more selected from the group consisting of vitamins, milk thistle extract, lutein, zeaxanthin, fish, omega-3 powder, hyaluronic acid, and berry extracts may be added.

Vitamin supplements may contain one or more selected from the group consisting of vitamin A, vitamin D, vitamin B1, vitamin B3, and vitamin B6. Among these, vitamin A is an essential nutrient that is involved in maintaining companion animals' eyesight, maintaining reproductive function (especially reproduction), regulating skeletal growth, and growth. Vitamin D has the function of regulating calcium and phosphorus metabolism, such as promoting the absorption of calcium and phosphorus in the small intestine, mobilizing calcium and phosphorus stored in the skeleton, and increasing the reabsorption of calcium or phosphorus (P) in the kidney. If vitamin D is insufficient, clinical symptoms such as rickets, osteoporosis, skeletal abnormalities, decreased appetite, decreased growth and feed efficiency, and decreased reproductive function may appear, and if it is overfed, decreased appetite, vomiting, polyuria, and severe renal dysfunction may appear. Based on 1L of water, 2~3mg of vitamin A, 0.01~0.0125mg of vitamin D, 4~5mg of vitamin B1, 10~12mg of vitamin B3, and 1~2mg of vitamin B6 can be added.

Milk thistle extract is an extract of the flower of the white-spotted thistle (Silybum marianum), an annual plant in the Asteraceae family. Its English name is milk thistle. There is a story that the name "milk" comes from the fact that it was eaten by mothers to increase milk production in the past. The milk thistle is excellent for protecting the liver by preventing glutathione (GSH) deficiency in the liver, and has the function of preventing or treating gallstones by promoting the flow of bile. Furthermore, it has the effect of alleviating a series of liver diseases including cirrhosis and viral hepatitis. It is also a more powerful antioxidant than vitamins C and E, and suppresses tissue damage from unstable free radical molecules. The milk thistle extract can be added at 50 to 200 mg per 1 L of water.

Xanthophylls are substances with C ₄₀ H ₅₆ O ₂ , and their chemical structure contains oxygen in the form of hydroxyl groups (-OH), carbonyl (-CO-), and epoxide (-O-) among carotenoids. Xanthophylls are widely present in the biological world and are produced by the oxygenation of carotenoids in the body. Animals cannot produce xanthophylls and obtain them through the intake of food. For example, the yellow color of egg yolks is due to the xanthophylls consumed by chickens. The xanthophylls group includes lutein, zeaxanthin, and α-cryptoxanthin, β-cryptoxanthin, and in a narrow sense, it refers to lutein.

Xanthophylls play a role in reducing age-related vision loss originating in the central part of the retina and prevent damage to retinal tissue caused by bright light. In particular, lutein, one of the xanthophylls, refers to lipochrome, a yellow pigment obtained from retinal cells and egg yolk, and is covalently bonded with xanthophylls. This lutein acts as an antioxidant that protects the inside of the eye from damage by oxygen free radicals naturally produced in the body. It is also reported to be a physiologically active substance that kills cancer cells by reducing the growth of blood vessels that supply cancerous tumors, and is effective in preventing breast, colon, lung, ovarian, and skin cancer.

And zeaxanthin, one of the xanthophylls, is one of the carotenoid alcohols and one of the two carotenoids contained in the retina, and is abundant in plants with colors such as corn, saffron, dark green leafy mustard, turnips, kale, and collards. Zeaxanthin is known to prevent vision loss and cataracts due to aging, prevent fatty acid peroxidation in the photoreceptor membrane, and suppress blood vessels supplying the retina. These lutein and zeaxanthin have recently been in the spotlight as health supplements with vision-enhancing effects. It is recommended to add both lutein and zeaxanthin, and 10 to 20 mg can be added per 1L of water.

Fish can be added to stimulate the appetite of felines, for example, raw salmon can be finely ground in a grinder and used, or oil containing salmon ingredients can be used. Vitamin A contained in such salmon can reduce allergic reactions, is effective in preventing or treating eye diseases, and can provide nutrition to the skin and hair to make rough skin shiny. 50 to 100 mg can be added per 1 L of water.

Omega-3 powder is an essential unsaturated fatty acid in the body, which reduces inflammation, strengthens cell membranes, and improves eye health. Omega-3 is composed of DHA and EPA, and among these, DHA is present in high concentrations in the retina, improving eye health through visual development and anti-inflammatory effects on the eye. 10 to 50 mg can be added per 1L of water.

Hyaluronic acid exhibits different physiological activities or functionality depending on its molecular weight. High molecular weight hyaluronic acid is used as a space-filler in the human body and has functions such as anti-angiogenic activity and immunosuppressive activity. Medium molecular weight hyaluronic acid exhibits immunostimulatory activity, and low molecular weight hyaluronic acid is known to be involved in apoptosis and protein induction. In addition, it has excellent moisturizing effects, lubricating effects against physical friction, and protective effects against invasion of bacteria, etc. 10 to 50 mg can be added per 1 L of water.

Also, bilberry (Vaccinium myrtillus fruit (Bilberry)), a type of berry, is the fruit of the bilberry plant that has been used as a herbal medicine and food ingredient for centuries in Europe. It has been used as an antioxidant and anti-inflammatory agent due to its high content of polyphenols, and has been reported to have a remarkable astringent effect, so it is used to treat inflammation of the mouth and mucous membranes, and is also effective in treating diarrhea and hemorrhoids. In addition, it contains anthocyanin, niacin, vitamins A and C, and inorganic salts such as potassium and calcium. 10 to 50 mg can be added per 1 L of water.

Example 2.

A medium consisting of 40 wt% glucose, 30 wt% yeast culture, 28.5 wt% corn starch, and 1.5 wt% glutamic acid was prepared.

Next, Lactobacillus plantarum and Bacillus amyloliquefaciencs were inoculated onto the medium, and then the medium was cultured at 35°C, an air injection amount of 0.4 vvm, and a rotation speed of 200 rpm for 24 hours to simultaneously culture anaerobic Lactobacillus plantarum and aerobic Bacillus amyloliquefaciencs, thereby culturing a composite strain culture having a Lactobacillus plantarum content of 2.9 x 10 ⁸ cfu/g and a Bacillus amyloliquefaciencs content of 2.8 x 10 ⁸ cfu/g. Thereafter, the composite strain culture was separated into strains and culture supernatant by centrifugation.

Next, 2 parts by weight of iron (Fe) was added to 100 parts by weight of the culture supernatant to form an inorganic mineral solution.

In addition, 5 parts by weight of glycine was added to 100 parts by weight of the culture supernatant, and then 5 parts by weight of glutamic acid was further added and dissolved to promote the organic passivation reaction of the glycine, thereby forming an amino acid solution.

Next, the inorganic mineral solution and amino acid solution were mixed and stirred in a weight ratio of 1:1 to prepare an organic mineral solution, which was then freeze-dried in a freeze dryer at -50°C for 24 hours to prepare a powder-type pet feed additive (organo-passivated glycine iron powder).

Next, 100 g of the organic glycine-passivated iron powder, 200 g of agar powder, 2 mg of vitamin A, 0.0125 mg of vitamin D, 4 mg of vitamin B1, 11 mg of vitamin B3, 1.5 mg of vitamin B6, 100 mg of milk thistle extract, 20 mg of lutein and zeaxanthin, 100 mg of salmon powder, 30 mg of omega-3 powder, 50 mg of hyaluronic acid, and 30 mg of bilberry extract were added to 1 L of water, and then mixed and stirred to form a gel-type mixture.

Thereafter, 0.5 parts by weight of sodium acetate was added to 100 parts by weight of the mixture to form a gel-type pet feed additive according to another embodiment of the present invention.

By providing the present invention configured as described above, the absorption rate of minerals in the pet's body is improved through the chelate bond of minerals and amino acids, thereby increasing the growth rate, and an improvement in immune function can be expected, and there is a remarkable effect of reducing the amount of minerals excreted from the body.

The terms and words used in this specification and claims described above should not be interpreted as limited to their usual or dictionary meanings, but should be interpreted as meanings and concepts that conform to the technical idea of the present invention based on the principle that the inventor can appropriately define the concept of the term in order to explain his own invention in the best way.

Therefore, the configurations depicted in the drawings and embodiments described in this specification are only the most preferred embodiment of the present invention, and do not represent all of the technical ideas of the present invention. Therefore, it should be understood that there may be various equivalents and modified examples that can replace them at the time of filing this application.

Claims (6)

A complex strain culture solution in which a medium comprising 94 to 98 wt% of purified water, 1 to 3 wt% of glucose, 0.5 to 2 wt% of yeast extract, 0.1 to 0.5 wt% of sodium chloride (NaCl), and 0.1 to 0.5 wt% of potassium hydrogen phosphate (K ₂ HPO ₄ ) is sterilized at 120 to 125°C for 15 minutes, lactic acid bacteria and Bacillus subtilis are inoculated into the medium, and cultured for 24 to 36 hours at a temperature of 30 to 37°C, an air injection amount of 0.2 to 0.4 vvm, and a rotation speed of 50 to 200 rpm. In order to promote the growth of the lactic acid bacteria and Bacillus subtilis, one or more of a seawater-derived mineral concentrate, an aqueous sulfur dispersion, or an aqueous illite dispersion is added to the medium to culture the complex strain culture solution. Incubation stage (S10) and;
A culture supernatant preparation step (S20) in which the above complex strain culture solution is separated into a complex strain and a culture supernatant, and the above culture supernatant is separately selected;
A step (S30) of forming an inorganic mineral solution by dissolving inorganic minerals in the culture supernatant;
An amino acid solution composition step (S40) for dissolving amino acids in the culture supernatant to create an amino acid solution;
An organic mineral solution composition step (S50) of mixing the above inorganic mineral solution and amino acid solution to form an organic mineral solution through a chelating reaction;
A method for manufacturing a pet feed additive, characterized by including a freeze-drying step (S60) of freeze-drying the organic mineral solution.
A step (S10) of culturing a complex strain culture solution, wherein a medium composed of 30 to 50 wt% of glucose, 20 to 40 wt% of yeast culture, 25 to 30 wt% of corn starch, and 1 to 5 wt% of glutamic acid is sterilized at 120 to 125°C for 15 minutes, lactic acid bacteria and Bacillus subtilis are inoculated into the medium, and then cultured at a temperature of 30 to 37°C, an air injection amount of 0.2 to 0.4 vvm, and a rotation speed of 50 to 200 rpm for 24 to 36 hours, and wherein one or more of a seawater-derived mineral concentrate, an aqueous sulfur dispersion, or an aqueous illite dispersion is added to the medium to promote the growth of the lactic acid bacteria and Bacillus subtilis, thereby culturing a complex strain culture solution;
A culture supernatant preparation step (S20) in which the above complex strain culture solution is separated into a complex strain and a culture supernatant, and the above culture supernatant is separately selected;
A step (S30) of forming an inorganic mineral solution by dissolving inorganic minerals in the culture supernatant;
An amino acid solution composition step (S40) for dissolving amino acids in the culture supernatant to create an amino acid solution;
An organic mineral solution composition step (S50) of mixing the above inorganic mineral solution and amino acid solution to form an organic mineral solution through a chelating reaction;
A method for manufacturing a pet feed additive, characterized by including a freeze-drying step (S60) of freeze-drying the organic mineral solution.
In the first or second paragraph, the step of forming the inorganic mineral solution (S30) is
In 100 parts by weight of the culture supernatant, 1 to 10 parts by weight of any one inorganic mineral selected from the group consisting of chromium (Cr), zinc (Zn), iron (Fe), copper (Cu), selenium (Se), manganese (Mn), boron (B), titanium (Ti), germanium (Ge), and calcium (Ca) is dissolved to form an inorganic mineral solution.
A method for manufacturing a pet feed additive, characterized in that an organic acid is further added to adjust the pH of the culture supernatant to promote the dissolution of the inorganic minerals.

In the first or second paragraph, the amino acid solution composition step (S40) is
A method for producing a pet feed additive, characterized in that 0.1 to 10 parts by weight of one amino acid selected from the group consisting of lysine, arginine, histidine, cysteine, phenylalanine, tyrosine, methionine, and glycine is dissolved in 100 parts by weight of the culture supernatant.
In the first or second paragraph, in the amino acid solution composition step (S40),
The above amino acid is glycine,
The above glycine is added in an amount of 0.1 to 10 parts by weight per 100 parts by weight of the culture supernatant.
A method for manufacturing a pet feed additive, characterized in that glutamic acid is further added to promote a chelating reaction with the above-mentioned inorganic mineral solution.
A method for manufacturing a pet feed additive, characterized in that it comprises a step of mixing and stirring the organic mineral powder manufactured in the freeze-drying step (S60), water, an excipient, a preservative, and a functional additive in claim 1 or 2.