KR102717830B1 - Cosmetic Composition Comprising Natural Softening Plants Prepared by Using Microorganism As Active Ingredient - Google Patents

Abstract

The present invention provides a cosmetic composition containing, as an effective ingredient, a natural softening plant using microorganisms that produce enzymes as metabolites. The natural softening plant using microorganisms can be produced by a method including a saccharification step of the plant, a freezing and crushing step, a softening step using a microbial culture solution, an ultraviolet irradiation step, and a filtration/washing step.

Description

Cosmetic composition comprising natural softening plants prepared by using microorganisms as active ingredients {Cosmetic Composition Comprising Natural Softening Plants Prepared by Using Microorganism As Active Ingredient}

The present invention relates to a cosmetic composition containing a natural softening plant as an effective ingredient using microorganisms that produce enzymes as metabolites.

Cosmetics are products that are applied, rubbed, or sprayed onto the body to cleanse and beautify the body, add charm, brighten the appearance, or maintain or improve the health of the skin or hair. They basically have functions such as moisturizing, whitening, and elasticity.

Today, the cosmetics industry is one of the industries that is rapidly developing and sensitive to trends and fads.

In particular, due to the prolonged COVID-19 pandemic and the incorporation of masks into daily life, basic skincare products are on the rise and products that can fundamentally improve the skin are in the spotlight. Accordingly, natural or organic ingredients, such as natural origin and natural origin, which mainly consist of skin soothing and regenerating ingredients, are trending. In addition, consumers who want to check the origin of the extracts that make up the ingredients, such as the source or geographical conditions, are increasing.

On the other hand, most of the cases are where the plant is extracted and applied to cosmetics as an extract, but in such cases, there is a problem that most of the effective ingredients are lost during the process of extracting the plant, so the effect is very small. In addition, when the plant is put into cosmetics as it is, there is a limitation that the skin effect that can be obtained from the plant in its original form is minimal, although it has an ornamental purpose.

Meanwhile, the cell wall of a plant has a complex structure composed of cellulose, hemicellulose, pectin, lignin, etc. When using a plant as a raw material for cosmetics, the cellulose is removed instead of being used directly. In this process, most of the effective ingredients that are not extracted by water or organic solvents are discarded along with the cellulose. Therefore, recently, there has been a demand for the development of materials that can maximize the effective ingredients of natural plant materials while also attracting the interest of consumers.

Meanwhile, softening refers to the phenomenon in which tissues become soft, and the polysaccharides that make up the cell walls of plants are involved in softening by being reduced to low molecular weight. Most domestic and international studies conducted to date have used commercial acids or enzymes to elucidate hydrolysis characteristics.

Korean Patent No. 10-2247990 discloses a natural leaf cosmetic composition manufactured by impregnating natural leaves in an aqueous solution containing acid and purified water.

Korean Patent No. 10-1047785 discloses a cosmetic composition containing natural softened petals manufactured through a softening process in which natural petals are mixed in an aqueous solution of citric acid, carbomer or a mixture thereof and then heated to 70 to 90°C.

Accordingly, the inventors of the present invention confirmed the softening effect of a plant body using an enzyme generated during the metabolic process of a microorganism, thereby completing the present invention.

The present invention aims to provide a cosmetic composition comprising a natural softening plant manufactured using microorganisms. Specifically, the present invention aims to provide a cosmetic composition containing the same by confirming that the softening process using a metabolite of microorganisms is environmentally friendly, harmless to the human body, and has an excellent softening effect.

To achieve the above purpose, the present invention provides a cosmetic composition comprising a natural softening plant body manufactured using microorganisms.

Preferably, the natural softening plant body using the microorganism is produced by a method including the steps of saccharifying the plant body; freezing and crushing the saccharified plant body using liquid nitrogen; softening the frozen and crushed plant body by adding a microbial culture solution; sterilizing the softened product by irradiating it with ultraviolet rays; and filtering and washing the sterilized softened product to obtain the softened plant body, wherein the microorganism is a microorganism that produces an enzyme as a metabolite.

Also preferably, the microorganism may be selected from the group consisting of Lactobacillus plantarum , Pediococcus genus, Enterococcus faecium , Pediococcus pentosaceus , Trichoderma viride , Clostridium thermocellum, Pseudomonas fluorescens , and Cellulomonas uda .

Also preferably, the natural softening plant using the microorganism may be contained in an amount of 0.1 to 30 wt% relative to the total weight of the cosmetic composition.

Also preferably, the saccharification step is to add at least one sugar selected from the group consisting of monosaccharides, disaccharides, polysaccharides and sugar alcohols to the plant in an amount of 1 to 20 wt% relative to the weight of the plant.

Also preferably, the freezing and crushing step is to freeze the saccharified plant body by placing it in liquid nitrogen and leaving it for 1 to 30 minutes, and crush it with a crusher to obtain uniform plant bodies of 1 to 10 mm in size.

Also preferably, the plant body may be a young branch, leaf, flower or fruit of a woody plant or a flower, leaf, stem, root or fruit of an herbaceous plant.

Also preferably, the ultraviolet irradiation step can be performed for 10 minutes to 1 hour using a 20W ultraviolet sterilizing lamp.

The cosmetic composition of the present invention has an excellent softening degree through a softening process of a plant using an enzyme produced through a metabolic process of microorganisms, so that the plant is included in the cosmetic composition while maintaining its original shape, and is gently massaged to the skin without a foreign body sensation, and provides the effect of maximally utilizing the effective ingredients of the plant. In addition, the natural softening plant obtained through the softening process using microorganisms is safe for the skin without irritation, and its inherent efficacy is increased through the interaction with the plant that occurs during the softening process using microorganisms, and provides the effect of obtaining a plant with reduced negative sensations of use, such as the generation of an off-flavor.

Figure 1 is a photograph of Example 1 of the present invention dispersed in a glycerin aqueous solution.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and are consistent with the meaning generally understood by those skilled in the art in the relevant field of the present invention. In addition, although preferred methods or samples are described in this specification, similar or equivalent ones are also included in the scope of the present invention.

The term "about" means an amount, level, value, number, frequency, percent, dimension, size, quantity, weight or length that varies by about 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, 4, 3, 2 or 1 percent of the reference amount, level, value, number, frequency, percent, dimension, size, quantity, weight or length.

Throughout this specification, unless the context otherwise requires, the words "comprise" and "comprising" will be understood to imply the inclusion of a stated step or component, or group of steps or components, but not the exclusion of any other step or component, or group of steps or components.

The term “softened plant” used in the present invention refers to a plant whose cell wall components have been changed by enzymes produced by microorganisms, causing the plant tissue to become soft.

The present invention relates to a cosmetic composition comprising a natural softening plant using microorganisms.

In general, when plants are used as they are in cosmetics, the complex structure of the plant cell wall, which is composed of cellulose, pectin, lignin, etc., makes the tissue tough and causes irritation to the skin due to the foreign body sensation, and the effect of delivering the effective ingredients in the plant to the skin is minimal. Therefore, a process is needed to break down the polysaccharides that compose the cell wall of the plant and reduce them to low molecular weight.

Meanwhile, the method using chemicals is mainly used for hydrolyzing polysaccharides, which can produce harmful byproducts and cause adverse effects on the skin, and not only does it incur costs in the waste disposal process, but there is also a risk of environmental pollution.

Therefore, the hydrolysis method of polysaccharides using metabolites produced by microorganisms can be used as an alternative to the chemical decomposition method described above. The hydrolysis method of polysaccharides using microorganisms has the advantage that the result is safe for the skin, environmentally friendly, and easy to mass-produce. In addition, useful metabolites produced by microorganisms can interact with plants to produce a synergistic effect.

In the present invention, a softening plant is produced using a microorganism that produces an enzyme as a metabolite, and a cosmetic composition containing the same is disclosed.

Preferably, a cellulase-producing microorganism is used as the microorganism that produces the enzyme as a metabolite, and preferably, a microorganism of the genus Pediococcus , Enterococcus , Lactobacillus , Trichoderma , Clostridum , Pseudomonas , or Cellulomanas can be used. More preferably, the microorganism may be selected from the group consisting of Lactobacillus plantarum , Pediococcus genus, Enterococcus faecium , Pediococcus pentosaceus , Trichoderma viride , Clostridium thermocellum, Pseudomonas fluorescens , and Cellulomonas uda .

In addition, the plant of the present invention can be applied to young branches, leaves, flowers, fruits, etc. of woody plants or flowers, leaves, stems, roots, fruits, etc. of herbaceous plants. However, the plant of the present invention is not limited thereto.

Meanwhile, in the present invention, softened dermis is manufactured using dermis, which is mainly used as a skin beauty material, and a cosmetic composition containing the same is disclosed.

Citrus peel refers to the mature peel of the citrus fruit ( Citrus unshiu M.) of the Rutaceae family or a closely related plant of the same genus. The main components of the citrus peel include naringin, hesperidin, vitamin C, folic acid, and pectin, and its pharmacological effects have been reported to include anti-inflammatory, anti-allergic, anti-bacterial, anti-wrinkle, and whitening effects.

According to one embodiment of the present invention, a natural softening plant using the microorganism is

(a) a step of washing and saccharifying the leaves, stems, roots, flowers, fruits, etc. of a plant to be included in a cosmetic composition;

(b) a step of freezing and crushing the saccharified plant body using liquid nitrogen;

(c) a step of softening the frozen and crushed plant body by placing it in a microbial culture solution;

(d) a step of sterilizing the softened material by irradiating it with ultraviolet rays; and

(e) It is manufactured by a method including the step of filtering and washing the sterilized softened material to obtain a softened plant body.

The saccharification treatment step of the above (a) can be performed by harvesting and washing the leaves, stems (branches), roots, flowers, etc. of the plant, adding 1 to 20 wt% of sugar relative to the total weight of the plant, and leaving the resulting mixture at room temperature for 0.5 to 6 hours.

The sugar above refers to a substance having a sweet taste in a general sense, and the sugar may be characterized by including at least one or more of a monosaccharide, a disaccharide, a polysaccharide, and a sugar alcohol. Preferably, the sugar may be sucrose, honey, or glycerol.

If the added sugar is less than 1 wt% of the total weight of the plant, there may be a problem in that saccharification of the plant is not evenly distributed, and conversely, if it exceeds 20 wt%, the efficiency is low compared to the amount of sugar input, making it uneconomical.

This saccharification step prevents the tissue cells of the plant from drying out and makes it easier to extract the active ingredients, so that they can be applied in the subsequent softening step.

The freeze-crushing step of the above (b) can be performed by freezing the saccharified plant body by placing it in liquid nitrogen for 1 to 30 minutes, and then crushing it with a crusher to obtain uniform plant bodies of 1 to 10 mm in size.

The above liquid nitrogen may be characterized by being liquefied nitrogen and having a temperature of -196°C under atmospheric pressure. In addition, in the step of freezing a plant using liquid nitrogen, if the plant is left for less than 1 minute, the plant may become incompletely frozen, and conversely, if the plant is left for more than 30 minutes, the plant tissue may be destroyed.

By this freeze-fracturing step, uniform plant bodies of 1 to 10 mm in size can be obtained, and there is an effect of preventing the loss of effective ingredients in the plant body in the subsequent softening step. In addition, there is an effect of removing fungi existing in or contaminated with the plant body itself, so that contamination that may occur in the subsequent microbial culture solution treatment step can be prevented.

The microbial culture step of the above (c) means culturing by adding 1 to 20 times the weight of the microbial culture solution to the saccharified and freeze-crushed plant body.

The above microorganism may be characterized as a microorganism that produces an enzyme as a metabolite.

Preferably, the microorganism may be selected and used from a group consisting of microorganisms that produce cellulase as a metabolite. Preferably, the microorganism may be selected from a group consisting of microorganisms of the genus Pediococcus , the genus Enterococcus , the genus Lactobacillus , the genus Trichoderma , the genus Clostridum , the genus Pseudomonas , and the genus Cellulomanas . More preferably, the microorganism may be selected from the group consisting of Lactobacillus plantarum , Pediococcus genus, Enterococcus faecium , Pediococcus pentosaceus , Trichoderma viride , Clostridium thermocellum, Pseudomonas fluorescens , and Cellulomonas uda .

It is characterized by performing a softening reaction in a fermentation tank at a temperature of 20 to 35℃ for 1 to 7 days. Since the fermentation conditions may vary depending on the microorganism, the contents of the present invention are not limited to the fermentation temperature and fermentation time exemplified above.

The sterilization step of the above (d) is characterized by irradiating the softened material with ultraviolet rays. Specifically, it can be characterized by irradiating for 10 minutes to 1 hour using an ultraviolet sterilizing lamp (20W).

The washing step of the above (e) is characterized by filtering the sterilized softened substance through a 400 mesh filter cloth and washing it several times with purified water to obtain a softened plant body.

The natural softening plant using the above microorganisms may be included in an amount of 0.1 to 30 wt% based on the total weight of the cosmetic composition.

When the content of the softening plant using the above microorganism is less than 0.1 wt%, it is difficult for the user to expect an effect due to the softening plant because the amount of the softening plant is so small when using the cosmetic, and when the content of the softening plant exceeds 30 wt%, the amount of effect that can be obtained is not proportional to the amount of the softening plant input, making it uneconomical compared to the amount input.

Hereinafter, the contents of the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to the following examples, and includes modifications of technical ideas equivalent thereto.

[Examples 1 to 7. Production of softened dermis using microorganisms]

As a natural plant, the tannery was prepared, and preprocessed using saccharification and liquid nitrogen to freeze-dry, and then the softened tannery of Examples 1 to 7 was manufactured through a softening process using microorganisms.

(1) Pretreatment of saccharification

Prepare 100 g of dried tangerine peel leaves, stir in 1,000 g of natural acacia honey, and completely soak. Then, saccharification was performed at room temperature for 3 hours. After saccharification was completed, saccharified tangerine peel was obtained by filtering using a strainer.

(2) Pretreatment for freeze fracturing using liquid nitrogen

Liquid nitrogen was poured into the prepared saccharified dermis so that the saccharified dermis was completely immersed in the liquid nitrogen. After that, the saccharified dermis was frozen by leaving it for 10 minutes. The frozen saccharified dermis was crushed using a crusher to obtain saccharified and frozen crushed dermis.

Through the processes (1) and (2) above, sugar is adsorbed to the plant tissue, and by leaving it in liquid nitrogen, the saccharified tissue can be easily broken down, thereby making it possible to make the hard plant tissue have a uniform particle size.

(3) Manufacturing of softened dermis with low molecular weight cellulose

The stock culture of each microorganism was inoculated onto a solid medium and cultured, and the formed colonies were inoculated onto a liquid medium and cultured until the absorbance values reached 1.8 to 2.0 at a wavelength of 600 nm for bacteria and 660 nm for fungi, thereby preparing a microbial culture. The appropriate medium and culture temperature for each type of microorganism are shown in Table 1 below, and the method for preparing each medium is based on the composition disclosed by the Korea Culture and Technology Center (KCTC).

division microorganism Badge name Incubation temperature Example 1 Lactobacillus plantarum MRS (De Man, Rogosa, Sharpe) 37℃ Example 2 Enterococcus faecium MRS 37℃ Example 3 Pediococcus pentosaceus MRS 37℃ Example 4 Trichoderma viride PD (Potato Dextrose) 25 ℃ Example 5 Clostridium thermocellum Clostridium Thermocellum medium 60℃ Example 6 Pseudomonas fluorescens TS (Tryptic Soy) 30℃ Example 7 Cellulomonas uda Nutrient 30℃

The microbial culture solution prepared above was added to the freeze-dried and crushed dermis in an amount 10 times the weight of the dermis, and the mixture was allowed to react in a fermentation tank to soften. The microorganisms used and the reaction conditions are shown in Table 2 below. After completion of the reaction, the mixture was sterilized by irradiating it with ultraviolet rays for 30 minutes under an ultraviolet sterilizing lamp (20 W). Thereafter, the mixture was filtered through a 400 mesh filter cloth and washed several times with purified water to obtain a softened dermis using the microorganisms of Examples 1 to 6.

Through the process of (3) above, the growth of microorganisms increases using glucose, etc. from saccharified plant tissues as nutrients, and ultimately, there is an effect of increasing the yield of softening.

division microorganism softening temperature Softening time Example 1 Lactobacillus plantarum 37℃ 20 hours Example 2 Enterococcus faecium 37℃ 30 hours Example 3 Pediococcus pentosaceus 37℃ 24 hours Example 4 Trichoderma viride 25 ℃ 40 hours Example 5 Clostridium thermocellum 60℃ 20 hours Example 6 Pseudomonas fluorescens 30℃ 34 hours Example 7 Cellulomonas uda 30℃ 24 hours

The softened dermis of Example 1 above dispersed in a glycerin aqueous solution is shown in Fig. 1. Through this, it was determined that the softened dermis prepared in the above Example could be applied to cosmetics.

[Comparative examples 1 and 2. Production of softened dermis using chemical methods]

As described in Example 1 above, a 1 w/w% cellulase aqueous solution in an amount 10 times greater than the weight of the dermis was added to the dermis that had undergone saccharification and freeze-fracture using liquid nitrogen, and the result was reacted at a temperature of 50°C for 3 hours. Thereafter, the resultant was filtered through a 400 mesh filter cloth and washed several times with purified water to obtain a softened dermis using cellulase, which was referred to as Comparative Example 1.

As described in Example 1 above, a 1 w/w% pectinase aqueous solution in an amount 10 times greater than the weight of the dermis was added to the dermis that had undergone saccharification and freeze-fracture using liquid nitrogen, and the result was reacted at 50°C for 3 hours. Thereafter, the dermis was filtered through a 400 mesh filter cloth and washed several times with purified water to obtain a softened dermis using pectinase, which was referred to as Comparative Example 2.

[Experimental Example 1. Softening Degree Measurement]

In order to confirm the degree of softening of the softened dermis resulting from Examples 1 to 7 and Comparative Examples 1 to 2, each softened dermis was placed on the back of the hand to check the properties, and when rubbed with an appropriate level of force using the user's fingers, the degree of softening of the leaf was observed, and the results are shown in Table 3 below. In addition, the degree of softening was expressed according to the following criteria.

<Evaluation criteria>

◎: It softens very well and disappears without leaving any residue even with relatively little force.

○: It softens well, but when a strong force is applied compared to ◎, it disappears without leaving any residue.

△: The softening is good, but when a strong force is applied compared to ○, it disappears without leaving any residue.

- : Not softened, residue remains

division Yeonhwado Example 1 ◎ Example 2 ○ Example 3 ○ Example 4 ◎ Example 5 ○ Example 6 ◎ Example 7 ○ Comparative Example 1 △ Comparative Example 2 -

As shown in Table 3 above, in the case of softening plants using microorganisms of Examples 1 to 7 according to the present invention, a superior softening degree was observed compared to Comparative Examples 1 to 2 using a chemical method. Through this, it was confirmed that the dermis was effectively softened using microorganisms. In addition, in the case of softening dermis using L. plantarum of Example 1, the maximum softening effect was confirmed in a short period of time.

[Experimental Example 3. Evaluation of total polyphenol and flavonoid content]

In this experimental example, the total polyphenol and flavonoid contents of the softened dermis prepared in Examples 1 to 7 and Comparative Examples 1 to 2 were measured using a colorimetric method. 1 g of each softened dermis was soaked in 10 g of 70% v/v ethanol for 3 hours, and the supernatant was concentrated under reduced pressure to use as a sample.

To measure the total polyphenol content, 100 ㎕ of 2% Na ₂ CO ₃ and 20 ㎕ of 50% Folin-Ciocalteu reagent were added to 20 ㎕ of the sample, and the reaction was performed in the dark for 30 minutes. The absorbance was measured at a wavelength of 750 nm using a microplate reader. The total polyphenol content in the sample was calculated as mg gallic acid equivalent (GAE)/g using a calibration curve generated using gallic acid at various concentrations, and is shown in Table 4 below.

The total flavonoid content was determined by adding 80 ㎕ of ethanol and 100 ㎕ of 2% AlCl ₃ ·6H ₂ O to 20 ㎕ of the sample, reacting for 5 minutes at room temperature, and measuring the absorbance at a wavelength of 450 nm using a microplate reader. The total flavonoid content in the sample was calculated as mg quercetin equivalent (QE)/g using a calibration curve created with quercetin at various concentrations, and is shown in Table 4 below.

Sample Total polyphenol content
(mg GAE/g) Total flavonoid content
(mg QE/g) Example 1 29.78 31.77 Example 2 20.51 14.72 Example 3 23.89 19.85 Example 4 22.61 26.54 Example 5 20.13 16.55 Example 6 17.88 20.98 Example 7 15.34 20.51 Comparative Example 1 1.61 1.38 Comparative Example 2 0.34 1.02

As shown in Table 4 above, the softened dermis of Examples 1 to 7 showed high total polyphenol and flavonoid contents, which is believed to be due to increased extraction of effective ingredients due to interaction with microorganisms.

[Experimental Example 3. Evaluation of Collagen Synthesis]

In this experimental example, the collagen synthesis effect was measured for the softened dermis prepared in Examples 1 to 7 and Comparative Examples 1 to 2 to confirm the skin wrinkle inhibition efficacy. 1 g of each softened dermis was placed in 10 g of 70% v/v ethanol, extracted for 3 hours, and the supernatant was concentrated under reduced pressure to use as a sample.

Human dermal fibroblast (HDFn) cells were seeded in a 24-well plate at a concentration of 1 x 10 ⁵ cells/well and stabilized in a 37°C, 5% CO ₂ incubator. The samples were diluted by concentration and treated simultaneously with FBS-free medium. The supernatant was collected after 24 hours, and the cytotoxicity was confirmed by treating the remaining cells with MTT solution. The procollagen type Ⅰ peptide EIA kit was used to confirm the amount of collagen produced using the collected supernatant. After the collagen ELISA assay, the amount of produced collagen was calculated by measuring the absorbance at a wavelength of 450 nm, and the collagen production (%) was calculated by comparing it with the control group. The results are shown in Table 5 below.

Sample Concentration (ug/ml) Collagen production (%) Unprocessed - 100 Example 1 30 121.8 100 134.1 Example 2 30 107.8 100 116.6 Example 3 30 106.9 100 111.3 Example 4 30 105.6 100 110.9 Example 5 30 119.0 100 128.6 Example 6 30 110.4 100 118.3 Example 7 30 118.1 100 120.5 Comparative Example 1 30 100.3 100 100.1 Comparative Example 2 30 100.5 100 105.3

As shown in Table 5 above, the softened dermis obtained in Examples 1 to 7 generally exhibited excellent collagen production efficacy, and it was confirmed that the amount of collagen production was proportional to the degree of softening. In addition, it was confirmed that the softened dermis of Example 1 exhibited the best effect.

[Manufacturing Example 1 and Comparative Manufacturing Examples 1 to 2. Manufacturing of a cosmetic composition containing softened dermis]

Cosmetic compositions containing 5.0 wt% of the softened dermis of Example 1 and Comparative Examples 1 to 2 were prepared with the compositions shown in Table 6 below. However, the present invention can be applied in various formulations, and this preparation example is not intended to limit the present invention but is merely intended to specifically explain it.

division Raw material name
(Unit: weight%) Manufacturing example 1 Comparative manufacturing example 1 Comparative manufacturing example 2 A Purified water to 100 to 100 to 100 Disodium EDTA 0.02 0.02 0.02 Carbomer 1 7.0 7.0 7.0 Carbomer 2 14.0 14.0 14.0 B Dipropylene glycol 1.0 1.0 1.0 glycerin 7.0 7.0 7.0 1,2-Hexanediol 2.0 2.0 2.0 alcohol 4.0 4.0 4.0 C Steartrimonium
Chloride 5.0 5.0 5.0 Example 1 5.0 - - Comparative Example 1 - 5.0 - Comparative Example 2 - - 5.0

[Experimental Example 3. Patch test]

In order to evaluate the skin safety of the cosmetic compositions of the above Manufacturing Example 1 and Comparative Manufacturing Examples 1 to 2, a patch test was performed as a primary skin irritation test.

Twenty adult men and women in their 20s to 40s without skin diseases were tested, and 25 ㎕ of each of the cosmetic compositions of Manufacturing Example 1 and Comparative Manufacturing Examples 1 and 2 was applied to the inner part of the forearm of IQ Ultimate™. The patches were applied for 24 hours, and the degree of irritation was visually evaluated by two experts 1 hour after patch removal, 24 hours after patch removal, and 48 hours after patch removal. The evaluation was made by experts according to the following evaluation criteria, and the results are shown in Table 7 below.

<Judging criteria>

- : Non-irritating

+ : mild irritation

++ : Moderate irritation

+++ : Strong irritation

division Judgment time Stimulus level (number of subjects) - + ++ +++ Total Manufacturing example 1 1 hour after patch removal 18 1 1 - 20 24 hours later 20 - - - 20 48 hours later 20 - - - 20 Comparative manufacturing example 1 1 hour after removing the patch 14 4 2 - 20 24 hours later 18 1 1 - 20 48 hours later 18 2 - - 20 Comparative manufacturing example 2 1 hour after patch removal 10 5 3 2 20 24 hours later 15 2 3 - 20 48 hours later 19 - 1 - 20

As shown in Table 7 above, it was confirmed that the cosmetic composition containing the softening plant using the microorganism of Example 1 had superior skin safety compared to the cosmetic composition containing the softening plant using a chemical method.

Claims (7)

A cosmetic composition containing a natural softening plant as an effective ingredient,
The above natural softening plant is,
The stage of saccharifying the plant body;
A step of freezing and crushing the above saccharified plant body using liquid nitrogen;
A step of softening by adding a microbial culture solution to the above-mentioned frozen and crushed plant body;
A step of sterilizing the above softened material by irradiating it with ultraviolet rays; and
It is manufactured by a method including the step of filtering and washing the sterilized softened material to obtain a natural softening plant body,
The above microorganism is a microorganism that produces enzymes as metabolites, and is Lactobacillus plantarum . Selected from the group consisting of Enterococcus faecium , Pediococcus pentosaceus , Trichoderma viride , Clostridium thermocellum , Pseudomonas fluorescens , and Cellulomonas uda ,
A cosmetic composition, characterized in that the natural softening plant using the above microorganism is contained in an amount of 0.1 to 30 wt% based on the total weight of the cosmetic composition. In the first paragraph,
A cosmetic composition, wherein the saccharifying step comprises adding at least one sugar selected from the group consisting of monosaccharides, disaccharides, polysaccharides and sugar alcohols to the plant in an amount of 1 to 20 wt% relative to the weight of the plant. In the first paragraph,
The above freezing and crushing steps are:
A cosmetic composition comprising a step of freezing the saccharified plant body by placing it in liquid nitrogen for 1 to 30 minutes, and crushing it with a crusher to obtain uniform plant bodies having a size of 1 to 10 mm. In the first paragraph,
A cosmetic composition, characterized in that the plant body is a young branch, leaf, flower or fruit of a woody plant, or a flower, leaf, stem, root or fruit of an herbaceous plant. In the first paragraph,
A cosmetic composition characterized in that the above ultraviolet irradiation step is performed using a 20W ultraviolet sterilizing lamp for 10 minutes to 1 hour. delete delete