KR102717772B1 - Health functional food composition for antioxidant activity and immune-enhancing - Google Patents

Abstract

The present invention relates to a health functional food composition for antioxidation and immunity enhancement, and more specifically, to a health functional food composition having a mixed composition of red beet concentrate powder, turmeric extract, red ginseng powder, vitamin B2, vitamin B1, nicotinamide, calcium pantothenate, vitamin B6, biotin, folic acid, sodium selenite mixed preparation, vitamin C, vitamin D3 mixed preparation, dried yeast, zinc oxide, manganese sulfate, hyaluronic acid mixed preparation, L-glutathione yeast extract, magnesium stearate, silicon dioxide, hydroxypropyl cellulose and refined glucose, and having an improved antioxidation and immunity enhancement effect, the composition comprising:

Description

{Health functional food composition for antioxidant activity and immune-enhancing}

The present invention relates to a health functional food composition for antioxidant and immune enhancement, and more specifically, to a functional composition having a mixed composition with enhanced antioxidant and immune enhancement effects, and a health functional food composition for antioxidant and immune enhancement containing the same.

Reactive oxygen species (ROS), such as hydrogen peroxide (H ₂ O ₂ ), superoxide anion (O ₂ ^- ), and hydroxyl radical (OH), which are inevitably generated as by-products of the process of consuming oxygen in the human body, cause toxicity due to their high reactivity in the body. These reactive oxygen species can be excessively produced due to various environmental or pathological factors, and their excessive production and deficiency of the antioxidant defense system that removes and defends against reactive oxygen species cause an imbalance in the antioxidant defense system, resulting in oxidative stress.

Immunity is a self-defense system that exists in the body. It is a process in which the body recognizes various substances or organisms that invade from the outside as foreign substances and eliminates and metabolizes them. It defends itself from damage caused by external stimuli or invasion by pathogenic microorganisms, but it can also damage its own tissues, such as in inflammatory reactions. It is divided into immune function suppression and immune function enhancement by controlling changes in immune function to restore it to normal or reduce the extent of change.

Cytokines are proteins secreted by cells involved in innate and adaptive immunity. They stimulate the growth and differentiation of lymphocytes during the activation phase of the adaptive immune response, and activate various types of effector cells during the operation phase of the innate and adaptive immune responses to eliminate microorganisms and antigens. Among them, TNF (tumor necrosis factor) is a cytokine that mediates and regulates innate immunity, and is mainly produced by activated macrophages. IL-6 (interleukin-6) is a cytokine that functions in both innate and adaptive immunity. In innate immunity, it induces the production of neutrophils from bone marrow progenitor cells, and in adaptive immunity, it stimulates the growth of B lymphocytes differentiated into antibody producers. It also stimulates the production of anti-inflammatory cytokines and inhibits the production and function of regulatory T lymphocytes, thereby promoting cell-mediated immune responses. It is produced by macrophages, endothelial cells, and T lymphocytes.

As we enter a busy modern society, many people, regardless of age, from children to test takers and the elderly, are suffering from chronic fatigue or having health problems due to weakened immunity. Stress, overwork, lack of sleep, excessive drinking, unbalanced diet, exposure to various environmental pollutants, etc. are known to be the main causes of weakened immunity. These problems gradually weaken the body's natural healing function, causing various diseases or symptoms such as atopic dermatitis, allergic rhinitis, urticaria, hypersensitivity pneumonia, otitis media, weak constitution, loss of appetite, growth disorder, decreased concentration, frequent colds, respiratory viruses, etc.

Currently, many vaccine adjuvants are being developed for the purpose of enhancing immunity, but only 5~6 adjuvants have actually been approved for use. The most widely used adjuvant, alum adjuvant, has relatively low immune enhancement efficacy and has been reported to have side effects such as autoimmune reactions. Due to safety issues with adjuvants, the use of relatively safe health functional foods is considered a more desirable option, and research on this is actively being conducted.

Accordingly, the inventors of the present invention have made efforts to develop a safe health functional food as an immune-enhancing agent, and as a result, they have manufactured a health functional food composition including red beet concentrate powder, turmeric extract, red ginseng powder, vitamin B2, vitamin B1, nicotinamide, calcium pantothenate, vitamin B6, biotin, folic acid, sodium selenite mixed preparation, vitamin C, vitamin D3 mixed preparation, dried yeast, zinc oxide, manganese sulfate, hyaluronic acid mixed preparation, L-glutathione yeast extract, magnesium stearate, silicon dioxide, hydroxypropyl cellulose, and refined glucose, and have confirmed a mixed composition of red beet concentrate powder, turmeric extract, red ginseng powder, and vitamin B2 that can further enhance the antioxidant and immune-enhancing effects of the composition. Accordingly, the inventors of the present invention have revealed that the health functional food composition can be usefully used as a health functional food composition for antioxidant and immune-enhancing effects, and have thus led to the present application.

Republic of Korea Patent No. 10-2270923

Cadenas E, Davies KJ. 2000. Mitochondrial free radical generation, oxidative stress, and aging. Free Radical Biology & Medicine 29:222-230 Bokov A, Chaudhuri A, Richardson A. 2004. The role of oxidative damage and stress in aging. Mechanisms of Aging and Development 125:811-826.

The purpose of the present invention is to provide a functional composition exhibiting antioxidant and immune system-improving effects and a health functional food composition containing the same.

In order to achieve the object of the present invention, the present invention provides an antioxidant and immune-enhancing health functional food composition comprising red beet concentrate powder, turmeric extract, red ginseng powder, vitamin B2, vitamin B1 or a salt thereof, nicotinamide, calcium pantothenate, vitamin B6 or a salt thereof, biotin, folic acid, sodium selenite mixed preparation, vitamin C, vitamin D3 mixed preparation, dried yeast, zinc oxide, manganese sulfate, hyaluronic acid mixed preparation, L-glutathione yeast extract, magnesium stearate, silicon dioxide, hydroxypropyl cellulose and refined glucose.

The health functional food composition according to the present invention comprises red beet concentrate powder, turmeric extract, red ginseng powder, vitamin B2, vitamin B1, nicotinamide, calcium pantothenate, vitamin B6, biotin, folic acid, sodium selenite mixed preparation, vitamin C, vitamin D3 mixed preparation, dried yeast, zinc oxide, manganese sulfate, hyaluronic acid mixed preparation, L-glutathione yeast extract, magnesium stearate, silicon dioxide, hydroxypropyl cellulose and refined glucose, and has a mixed composition of red beet concentrate powder, turmeric extract, red ginseng powder and vitamin B2 with enhanced antioxidant and immune-enhancing effects, and can be usefully used as a health functional food for antioxidant and immune-enhancing effects.

Figure 1 is a diagram confirming cell viability according to treatment with a composition manufactured according to one embodiment of the present invention in RAW 264.7 cells.
FIG. 2 is a diagram confirming the NO concentration present in a cell culture of RAW 264.7 cells treated with a composition manufactured according to one embodiment of the present invention.
FIG. 3 is a diagram confirming the concentration of TNF-α present in a cell culture of RAW 264.7 cells treated with a composition manufactured according to one embodiment of the present invention.
FIG. 4 is a diagram confirming the concentration of IL-6 present in a cell culture of RAW 264.7 cells treated with a composition manufactured according to one embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention provides an antioxidant and immune-enhancing health functional food composition comprising red beet concentrate powder, turmeric extract, red ginseng powder, vitamin B2, vitamin B1 or a salt thereof, nicotinamide, calcium pantothenate, vitamin B6 or a salt thereof, biotin, folic acid, a sodium selenite mixed preparation, vitamin C, a vitamin D3 mixed preparation, dried yeast, zinc oxide, manganese sulfate, a hyaluronic acid mixed preparation, L-glutathione yeast extract, magnesium stearate, silicon dioxide, hydroxypropyl cellulose, and refined glucose.

In the present invention, the red beet concentrate powder contains anthocyanin and betanin as active ingredients, and is rich in various vitamins and minerals, thereby helping to enhance antioxidation and immunity. The red beet concentrate powder may be used without limitation in either commercially available or manufactured products. If the red beet concentrate powder is manufactured, the specific manufacturing method is not limited, but as an example, washed red beets may be extracted and concentrated using a low-temperature pressing method, for example, at 45 to 50°C for 24 to 48 hours, and then powdered. In addition, the red beet concentrate powder may be included in an amount of 8 to 12 parts by weight, and specifically, may be included in an amount of 9 to 11 parts by weight.

In the present invention, the turmeric extract contains curcumin, a polyphenol component, which helps to enhance antioxidation and immunity. The turmeric extract may be used without limitation in a commercially available or manufactured one. If the turmeric extract is manufactured, the specific manufacturing method thereof is not limited, but for example, the extract may be extracted with water, a lower alcohol having 1 to 6 carbon atoms, or a mixture thereof, and more specifically, may be extracted with water. In addition, the extract may be extracted at 10° C. to 100° C. for 1 to 72 hours, filtered, and the filtered extract may be concentrated under reduced pressure using a vacuum rotary concentrator to be powdered. In addition, the turmeric extract may be included in an amount of 8 to 12 parts by weight, and specifically, may be included in an amount of 9 to 11 parts by weight.

In the present invention, the red ginseng powder can promote innate immunity by inducing the activation of macrophages by including red ginseng polysaccharide. In addition, the red ginseng powder can be included in an amount of 0.005 to 0.025 parts by weight, and can be included in an amount of 0.01 to 0.02 parts by weight.

In the present invention, the vitamin B2 is necessary for energy production in the body, and acts as a photosensitizer, thereby having a photodynamic pathogenic reduction effect, that is, a bacterial and viral extermination and annihilation effect. In addition, the vitamin B2 is characterized by having the highest content among those available on the market within the allowable range of the Ministry of Food and Drug Safety. Specifically, the vitamin B2 may be included in an amount of 38 to 42 parts by weight, and more specifically, may be included in an amount of 39 to 41 parts by weight.

Specifically, the composition contains 8 to 12 parts by weight of red beet concentrate powder, 8 to 12 parts by weight of turmeric extract, 0.005 to 0.025 parts by weight of red ginseng powder, and 38 to 42 parts by weight of vitamin B2, and more specifically, it may contain 9 to 11 parts by weight of red beet concentrate powder, 9 to 11 parts by weight of turmeric extract, 0.01 to 0.02 parts by weight of red ginseng powder, and 39 to 41 parts by weight of vitamin B2, and a synergistic effect of antioxidant and immunity improvement can be obtained through the mixed composition. Accordingly, when the contents of red beet concentrate powder, turmeric extract, red ginseng powder, and vitamin B2 in the composition of the present invention are out of the above range, the antioxidant effect is reduced, the activity of macrophages is reduced, and the improvement of immunity is reduced.

In the present invention, the vitamin B1 is necessary for carbohydrates and energy targets. The composition may be included as vitamin B1 or a salt of vitamin B1, and for example, vitamin B1 hydrochloride may be included as a salt of vitamin B1. In addition, the vitamin B1 or a salt thereof may be included in an amount of 0.15 to 0.35 parts by weight, and specifically, may be included in an amount of 0.2 to 0.3 parts by weight.

In the present invention, the nicotinic acid amide is also known as vitamin B3 and is necessary for energy production in the body together with vitamin B2. In addition, the nicotinic acid amide may be included in an amount of 1.5 to 3.5 parts by weight, and specifically, may be included in an amount of 2 to 3 parts by weight.

In the present invention, the calcium pantothenate is known as vitamin B5, and is necessary for fat, carbohydrate, and protein metabolism and energy production, and helps secretion of hormones that control inflammation and enhance immunity. In addition, the calcium pantothenate may be included in an amount of 0.6 to 1 part by weight, and may be included in an amount of 0.8 to 1 part by weight.

In the present invention, the vitamin B6 is necessary for the utilization of proteins and amino acids, and is necessary for maintaining the homocysteine level of blood at a normal level. The composition may include vitamin B6 or a salt of vitamin B6, and for example, vitamin B6 hydrochloride may be included as a salt of vitamin B6. In addition, the vitamin B6 or a salt thereof may be included in an amount of 0.2 to 0.35 parts by weight, and specifically, may be included in an amount of 0.2 to 0.3 parts by weight.

In the present invention, the biotin is necessary for fat, carbohydrate, protein metabolism and energy production. In addition, the biotin may be included in an amount of 0.003 to 0.006 parts by weight, and specifically, may be included in an amount of 0.004 to 0.005 parts by weight.

In the present invention, the folic acid is necessary for cell and blood production and maintaining the homocysteine level of blood at normal levels. In addition, the folic acid may be included in an amount of 0.03 to 0.1 parts by weight, and specifically, may be included in an amount of 0.05 to 0.1 parts by weight.

In the present invention, the sodium selenite mixture is composed of sodium selenite and dibasic calcium phosphate, and a commercial product can be purchased and used. The sodium selenite is an inorganic selenium, and has antioxidant, immune function, and anticancer effects. In addition, the sodium selenite mixture can be included in an amount of 0.6 to 1 part by weight, and specifically, can be included in an amount of 0.7 to 0.9 parts by weight.

In the present invention, the vitamin C is necessary for the formation and maintenance of connective tissue function, absorption of iron, and has an antioxidant effect. In addition, the vitamin C may be included in an amount of 13 to 17 parts by weight, and specifically, may be included in an amount of 14 to 16 parts by weight.

In the present invention, the vitamin D3 mixed preparation is composed of vitamin D3, medium-chain triglycerides, and dl-α-tocopherol, and a commercial product can be purchased and used. The vitamin D3 helps calcium absorption and regulates immune function. In addition, the vitamin D3 mixed preparation can be included in an amount of 0.3 to 1 part by weight, and can be included in an amount of 0.5 to 0.8 parts by weight.

In the present invention, the dry yeast contains chromium, and a commercially available product can be purchased and used. The chromium is involved in carbohydrate, fat, and protein metabolism in the body. In addition, the dry yeast can be included in an amount of 1.5 to 3.5 parts by weight, and specifically, can be included in an amount of 2 to 3 parts by weight.

In the present invention, the zinc oxide is necessary for normal immune function and cell division. In addition, the zinc oxide may be included in an amount of 1 to 2.5 parts by weight, and specifically, may be included in an amount of 1 to 2 parts by weight.

In the present invention, the manganese sulfate is involved in metabolism and is necessary for normal functioning of various organs such as the brain and liver. The composition may contain manganese gluconate, manganese chloride or manganese citrate instead of manganese sulfate. In addition, the manganese sulfate may be included in an amount of 1 to 2.5 parts by weight, and specifically, may be included in an amount of 1 to 2 parts by weight.

In the present invention, the hyaluronic acid mixture is composed of dextrin and hyaluronic acid, and a commercially available product can be purchased and used. The hyaluronic acid helps moisturize the skin. In addition, the hyaluronic acid mixture can be included in an amount of 0.0005 to 0.002 parts by weight, and specifically, can be included in an amount of 0.0007 to 0.0015 parts by weight.

In the present invention, the L-glutathione yeast extract is a substance in which glycine, cysteine, and glutamic acid are combined, has an antioxidant effect, and helps the metabolic process of the liver. In addition, the L-glutathione yeast extract may be included in an amount of 0.0005 to 0.002 parts by weight, and specifically, may be included in an amount of 0.0007 to 0.0015 parts by weight.

In the present invention, the magnesium stearate may be included in an amount of 0.5 to 1.5 parts by weight, and specifically, may be included in an amount of 0.5 to 1 part by weight.

In the present invention, the silicon dioxide has the effect of enhancing immunity and improving chronic fatigue. In addition, the silicon dioxide may be included in an amount of 0.5 to 1.5 parts by weight, and specifically, may be included in an amount of 0.5 to 1 part by weight.

In the present invention, the hydroxypropyl cellulose may be included in an amount of 0.5 to 1.5 parts by weight, and specifically, may be included in an amount of 0.5 to 1 part by weight.

In the present invention, the purified glucose increases brain activity, improves concentration, improves fatigue recovery and stamina, activates metabolism, and helps muscle growth in growing infants, children, and adolescents. In addition, the purified glucose may be included in an amount of 9 to 12 parts by weight.

Specifically, the composition comprises 0.15 to 0.35 parts by weight of vitamin B1 or a salt thereof, 1.5 to 3.5 parts by weight of nicotinamide, 0.6 to 1 part by weight of calcium pantothenate, 0.2 to 0.35 parts by weight of vitamin B6 or a salt thereof, 0.003 to 0.006 parts by weight of biotin, 0.03 to 0.1 parts by weight of folic acid, 0.6 to 1 part by weight of a sodium selenite mixture, 13 to 17 parts by weight of vitamin C, 0.3 to 1 part by weight of a vitamin D3 mixture, 1.5 to 3.5 parts by weight of dried yeast, 1 to 2.5 parts by weight of zinc oxide, 1 to 2.5 parts by weight of manganese sulfate, 0.0005 to 0.002 parts by weight of a hyaluronic acid mixture, and L-glutathione yeast extract. It may include 0.0005 to 0.002 parts by weight of sodium bicarbonate, 0.5 to 1.5 parts by weight of magnesium stearate, 0.5 to 1.5 parts by weight of silicon dioxide, 0.5 to 1.5 parts by weight of hydroxypropyl cellulose, and 9 to 12 parts by weight of refined glucose.

That is, the health functional food of the present invention, composed as described above, not only helps the body's carbohydrate, fat, and protein metabolism and energy production, but also has improved antioxidant and immunity-boosting effects.

In addition, all ingredients contained in the health functional food of the present invention are used as food materials and foods, are non-cytotoxic, and are excellently safe for the human body when taken or consumed.

The health supplement of the present invention can be formulated in the form of powder, capsule, powder, tablet, granule, extract or beverage, etc., according to a conventional manufacturing method. At this time, it goes without saying that a known food additive may be further added for formulation.

The health supplement of the present invention can be taken in an appropriate amount according to age and weight, and sufficient effects can be obtained by taking 500 mg to 4000 mg per day. The above-mentioned amount can be taken once or in two or more divided doses, and can be eaten by chewing it directly, or taken with water or milk.

Hereinafter, the present invention will be described in detail by examples.

However, the following examples are only intended to illustrate the present invention, and the content of the present invention is not limited to the following examples.

<Example 1 and Comparative Examples 1 to 4> Preparation of health functional food composition for enhancing immunity

Each component was mixed according to the composition in Table 1 below, and pressed into tablets (700 mg per tablet) using a tablet press.

division Composition ratio (weight%) Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Red beet concentrate powder 10 - 10 - 5 15 turmeric extract 10 - - 10 5 15 Red ginseng powder 0.015 - - - 0.015 0.015 Vitamin B2 40 60 50 50 50 30 Vitamin B1 0.25 0.25 0.25 0.25 0.25 0.25 Nicotinamide 2.5 2.5 2.5 2.5 2.5 2.5 Calcium pantothenate 0.85 0.85 0.85 0.85 0.85 0.85 Vitamin B6 0.3 0.3 0.3 0.3 0.3 0.3 Biotin 0.0045 0.0045 0.0045 0.0045 0.0045 0.0045 Folic acid 0.06 0.06 0.06 0.06 0.06 0.06 Sodium selenite mixture 0.8 0.8 0.8 0.8 0.8 0.8 Vitamin C 15 15 15 15 15 15 Vitamin D3 Mixture 0.65 0.65 0.65 0.65 0.65 0.65 Dry yeast 2.5 2.5 2.5 2.5 2.5 2.5 Zinc oxide 1.6 1.6 1.6 1.6 1.6 1.6 manganese sulfate 1.5 1.5 1.5 1.5 1.5 1.5 Hyaluronic acid mixture preparation 0.001 0.001 0.001 0.001 0.001 0.001 L-Glutathione Yeast Extract 0.001 0.001 0.001 0.001 0.001 0.001 Magnesium stearate 1 1 1 1 1 1 Silicon dioxide 1 1 1 1 1 1 Hydroxypropyl cellulose 1 1 1 1 1 1 Refined glucose 10.9685 10.9835 10.9835 10.9835 10.9685 10.9685

<Experimental Example 1> Cytotoxicity Evaluation

The cytotoxicity of the above Example 1 and Comparative Examples 1 to 4 was evaluated.

Specifically, RAW 264.7 cells were seeded at 2 × 10 ⁴ cells/well in 96-well plates (100 μl) and cultured for 24 hours in a 37°C, 5% CO ₂ incubator. The cells were treated with 100 μg/mL of the sample and cultured for 24 hours. In addition, the normal control group was treated with 0 μg/mL of the sample, and the positive control group was treated with 1 μg/mL of lipopolysaccharide (LPS). After that, 100 μl of 0.2 mg/mL MTT solution was added to each well and cultured for 2 hours. The supernatant was removed, and 100 μl of DMSO was added to thoroughly dissolve the formazan, and the absorbance was measured at 570 nm.

As a result, as shown in Fig. 1, it was confirmed that none of Example 1 and Comparative Examples 1 to 4 exhibited cytotoxicity.

<Experimental Example 2> Measurement of NO production

Macrophages are immune cells that play an important role in the innate immune system. When activated, they are not only significantly involved in promoting the body's immunity through phagocytosis, removal of antigen-antibody complexes, and regulation of the innate immune system, but also increase the production of reactive oxygen species and nitric oxide (NO) to remove pathogens and antigens that have invaded from the outside, and macrophages act as antigen-presenting cells to induce an antigen-specific acquired immune system. Accordingly, in order to determine the effect of Example 1 and Comparative Examples 1 to 4 on immune activity, the concentration of NO ^2- produced in a cell culture solution using macrophages was measured.

Specifically, RAW 264.7 cells were seeded in a 6-well plate at a density of 5 × 10 ⁵ cells/well and cultured for 24 hours, after which each prepared sample was treated at 100 ug/mL. The normal control group was treated with 0 ug/mL of the above sample, and the positive control group was treated with 1 ug/mL of lipopolysaccharide (LPS) and cultured for 24 hours. The supernatant of the cell culture medium and an equal amount of Griess reagent were mixed and reacted at room temperature for 15 minutes, and the absorbance was measured at 540 nm. The nitric oxide concentration was calculated from a standard curve created using sodium nitrite and converted.

As a result, as shown in Fig. 2, it was confirmed that NO production was promoted in Example 1 compared to the control group. In addition, it was confirmed that a synergistic effect of NO production was observed in the mixed composition of Example 1 compared to Comparative Examples 1 to 5.

<Experimental Example 3> Measurement of cytokine production

Activated macrophages secrete various cytokines such as IL-1, IL-6, and TNF-α, thereby activating the adaptive immune system. Accordingly, in order to determine the effects of Example 1 and Comparative Examples 1 to 4 on immune activity, cytokines TNF-α and IL-6 secreted in cell culture media using macrophages were measured.

Specifically, RAW 264.7 cells were treated with a sample using the same method as described in <Experimental Example 2> above, and then cell supernatants were obtained. Then, the concentrations of TNF-α and IL-6 were measured using TNF-α and IL-6 ELISA kits (BD Bioscience, USA) according to the manufacturer's procedure.

As a result, as shown in FIGS. 3 and 4, it was confirmed that TNF-α and IL-6 production increased compared to the control group in Example 1. In addition, it was confirmed that a synergistic effect on TNF-α and IL-6 production was observed in the mixed composition of Example 1 compared to Comparative Examples 1 to 5.

<Experimental Example 4> Antioxidant activity evaluation

The antioxidant activity of the above Example 1 and Comparative Examples 1 to 5 was confirmed.

Specifically, in order to evaluate the antioxidant activity of Example 1 and Comparative Examples 1 to 5, a DPPH radical scavenging activity experiment was conducted. The DPPH radical scavenging activity was measured by the method of Sharma OP, Bhat TK (2009). The sample was dissolved in 0.2% DMSO, and 0.2 mL of the sample solution was added to 0.8 mL of a 0.2 mM DPPH solution, mixed, and reacted at room temperature for 30 minutes, after which the absorbance was measured at 517 nm. The antioxidant power was calculated as the electron donating ability (%) by the calculation formula [1-(absorbance of the sample addition group/absorbance of the non-added group)] × 10.

division DPPH Radical scavenging activity (%)
(100 mg/100 mL) Example 1 94.77±0.23 Comparative Example 1 65.96±0.58 Comparative Example 2 56.49±0.08 Comparative Example 3 64.85±0.41 Comparative Example 4 62.63±0.24 Comparative Example 4 72.96±0.35

As a result, as shown in Table 2, it was confirmed that the antioxidant effect was significantly superior in Example 1 compared to Comparative Examples 1 to 5.

While the specific parts of the present invention have been described in detail above, it is obvious to those skilled in the art that such specific descriptions are merely preferred embodiments and that the scope of the present invention is not limited thereto. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

Red beet concentrate powder 8 to 12 parts by weight, turmeric extract 8 to 12 parts by weight, red ginseng powder 0.005 to 0.025 parts by weight, vitamin B2 38 to 42 parts by weight, vitamin B1 or a salt thereof 0.15 to 0.35 parts by weight, nicotinamide 1.5 to 3.5 parts by weight, calcium pantothenate 0.6 to 1 part by weight, vitamin B6 or a salt thereof 0.2 to 0.35 parts by weight, biotin 0.003 to 0.006 parts by weight, folic acid 0.03 to 0.1 part by weight, sodium selenite mixed preparation 0.6 to 1 part by weight, vitamin C 13 to 17 parts by weight, vitamin D3 mixed preparation 0.3 to 1 part by weight, dried yeast 1.5 to 3.5 parts by weight, zinc oxide 1 to An antioxidant and immune-enhancing health functional food composition comprising 2.5 parts by weight of manganese sulfate, 1 to 2.5 parts by weight of a hyaluronic acid mixture, 0.0005 to 0.002 parts by weight of L-glutathione yeast extract, 0.5 to 1.5 parts by weight of magnesium stearate, 0.5 to 1.5 parts by weight of silicon dioxide, 0.5 to 1.5 parts by weight of hydroxypropyl cellulose, and 9 to 12 parts by weight of refined glucose.
delete delete A health functional food composition for antioxidant and immune enhancement, wherein the salt of vitamin B1 in claim 1 is vitamin B1 hydrochloride.
A health functional food composition for antioxidant and immune enhancement, wherein the salt of vitamin B6 in claim 1 is vitamin B6 hydrochloride.
A health functional food composition for antioxidant and immune enhancement, wherein the composition in claim 1 is formulated in the form of a powder, capsule, powder, tablet, granule, extract or beverage.