KR102704040B1 - Cosmetic composition containing five varieties of fermented plant extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed extract of plants as an active ingredient, and more particularly, to the production of a cosmetic composition containing a mixed extract of okra, molokia, oats, wheat and carob beans as an active ingredient, which exhibits excellent skin moisturizing, skin barrier strengthening and skin texture improvement effects.

Description

Cosmetic composition containing five varieties of fermented plant extracts for improving skin texture and strengthening skin barrier

The present invention relates to a cosmetic composition containing a mixed extract of plants as an active ingredient, and more particularly, to the production of a cosmetic composition containing a mixed extract of okra, molokia, oats, wheat and carob beans as an active ingredient, which exhibits excellent skin moisturizing, skin barrier strengthening and skin texture improvement effects.

The skin, an organ that protects the body from the external environment, is divided into several layers. The skin is largely divided into three layers: the epidermis, dermis, and subcutaneous fat layer. The epidermis, which is the outermost layer, is further divided into four layers: the stratum corneum, the granular layer, the spinous layer, and the basal layer. The stratum corneum, which is the outermost layer of the skin, contains 15-20% of moisture, and the sebum film secreted from the sebum and sweat glands covers the stratum corneum and keeps it shiny. When the moisture content of the stratum corneum drops below 10%, the skin becomes dry and loses elasticity, causing wrinkles. Therefore, it is important to prevent moisture loss from the skin.

In general, alcohols, water-soluble polymers, and organic acid salts are used as moisturizers in cosmetics, and occlusive ingredients that have oiliness and prevent moisture evaporation from the skin surface and humectants that have moisture-absorbing properties and increase moisture retention in the stratum corneum are widely used.

In addition, the stratum corneum, located at the outermost layer of the skin, plays an important role in protecting the skin from various external stimuli and pollutants that affect the skin. Normally, stratum corneum naturally peels off over a certain period of time, and a new stratum corneum is formed. However, if stratum corneum peels off abnormally due to various factors such as aging, ultraviolet rays, and stress, the skin tone becomes uneven, making the skin dull, and causing various skin troubles such as acne. To solve this problem, various exfoliating cosmetics that help exfoliate stratum corneum are being developed.

Against this backdrop, the inventors of the present invention searched for raw materials that can help with moisturizing, strengthening the skin barrier, and improving skin texture, and found that by extracting okra and molokia containing polysaccharides called plant mucins and oats, wheat, and carob beans rich in nutrients by a specific method, an extract containing a large amount of organic acids and amino acids, which are useful ingredients for improving the skin, can be obtained, and it was confirmed that this can be usefully used in cosmetics for moisturizing the skin, improving skin texture, and strengthening the skin barrier, thereby completing the present invention.

(0001) Republic of Korea Publication Patent No. 10-2021-0003390 (2021.01.12) (0002) Republic of Korea Publication Patent No. 10-2023-0027503 (2023.02.28) (0003) Republic of Korea Patent No. 10-1308669 (2013.09.09)

The purpose of the present invention is to provide a cosmetic composition containing a mixed extract of okra, molokia, oats, wheat and carob beans as an effective ingredient, which exhibits excellent skin moisturizing, skin barrier strengthening and skin texture improvement effects.

In addition, another purpose of the present invention is to provide a method for producing the mixed extract.

In order to achieve the above object, according to the present invention, a cosmetic composition containing a mixed extract of okra, molokia, oats, wheat and carob beans as an effective ingredient is provided.

Preferably, the mixed extract is characterized by being a mixture of a first extract obtained by immersing a mixture of okra, molokia, oats, wheat and carob beans in a solvent and extracting at a low temperature of 30 to 40°C, and a second extract obtained by fermenting and extracting the residue remaining after preparing the first extract with lactic acid bacteria.

At this time, at least one selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol, and butylene glycol is used as the solvent.

The above lactic acid bacteria is at least one selected from the group consisting of Lactobacillus plantarum, Lactobacillus lactis, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Pediococcus cerevisiae, Lactobacillus breve, Lactobacillus sakei, and Lactobacillus crispatus, and more preferably Lactobacillus plantarum.

Preferably, the mixed extract is manufactured by extracting okra, molokhia, oats, wheat, and carob beans mixed in a weight ratio of 1 to 2:1 to 2:1 to 2:1 to 2:1 to 2:1 to 2, respectively.

The above mixed extract as an effective ingredient is contained in an amount of 0.01 to 50 wt% based on the total weight of the composition.

At this time, preferably, the mixed extract is formed by mixing the first extract and the second extract in a weight ratio of 1 to 5:1 to 5, more preferably in a weight ratio of 1 to 2:1 to 2, and most preferably in a weight ratio of 2:1.

The above cosmetic composition is characterized by being for moisturizing the skin, strengthening the skin barrier, or improving skin texture.

In order to achieve the other objects described above, according to the present invention, (A) a1) a step of immersing a mixture of okra, molokia, oats, wheat and carob beans in at least one solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol and extracting at a low temperature of 30 to 40°C for 2 to 3 hours; a2) a step of filtering to prepare a first extract;

(B) b1) a step of recovering the okra, molokia, oat, wheat and carob bean residues remaining after preparing the extract in the above step (A); b2) a step of immersing the recovered residues in a solvent, inoculating them with lactic acid bacteria and fermenting them at 25-30°C for 22-26 hours; b3) a step of extracting the fermented product at 80-100°C for 2-3 hours; b4) a step of filtering to prepare a second extract; and

(C) A method for producing a mixed extract of okra, molokia, oats, wheat and carob beans is provided, comprising a step of mixing the first extract of step (A) and the second extract of step (B) obtained above.

At this time, the lactic acid bacteria used for fermentation is at least one selected from the group consisting of Lactobacillus plantarum, Lactobacillus lactis, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Pediococcus cerevisiae, Lactobacillus breve, Lactobacillus sakei, and Lactobacillus crispatus.

The plant mixed extract manufactured by the manufacturing method of the present invention contains a high content of organic acid and amino acid components, and a cosmetic composition containing the same exhibits excellent moisturizing effects, skin barrier strengthening effects, and skin texture improvement effects.

The present invention will be described in more detail below.

The present invention is characterized in that by extracting okra, molokhia, oats, wheat and carob beans by a specific method, an extract containing a high content of organic acids and amino acids, which are useful ingredients for improving the skin, can be obtained, and when this is used as a cosmetic, excellent moisturizing, skin barrier strengthening and skin texture improvement effects can be obtained.

According to the present invention, a cosmetic composition containing a mixed extract of okra, molokia, oats, wheat and carob beans as an effective ingredient is provided.

Okra is a subtropical perennial herb in the Malvaceae family, native to Africa, and is also called Lady's finger because it resembles a woman's finger. Okra has a long history of cultivation, and young pods have been collected and used for food since ancient times. Nutritionally, it is a highly nutritious green vegetable that contains 90% moisture, 15% protein, 0.2% fat, 0.7% inorganic salts, 1.2% fiber, 6.4% carbohydrates, 88 mg of vitamin A, and 13 mg of vitamin C. In addition, it is known to have beneficial effects such as maintaining skin elasticity because it contains mucin, a mucus substance.

Molokhia, also called moroheiya or jew's mellow, is a green-yellow vegetable belonging to the Pinaceae family, and is a plant native to the Mediterranean coast of Egypt. In Egypt and the Middle East, young leaves are harvested and used for food. Nutritionally, it contains 36.7% protein, 29.7% crude fiber, 22% viscous polysaccharide, and 11.1% ash, and is also rich in vitamins B1, B2, C, and E, and minerals such as potassium, calcium, phosphorus, and iron, and is particularly rich in β-carotene and lutein. Molokhia leaves or whole plant can be used in the present invention.

Oats are a biennial cultivated plant of the gramineae family, and are called oats, oats, and yibak or oats. They are mainly produced in central and northern Europe including Russia, northern America, and southern Canada, and are known as a nutritious grain in Europe. They are the only superfood among whole grains, and are rich in dietary fiber, vitamins, lysine, tryptophan, calcium, potassium, iron, magnesium, copper, and zinc.

Wheat is a crop belonging to the family of grasses, also called barley, and is a crop with a long history of cultivation in Egypt and Western Asia. Based on the origin of agriculture, it is considered one of the world's three major crops along with rice and barley, and is considered a very important food ingredient. In addition to the nutritional components of wheat, it contains 27.9% protein, 12.7% vitamin E, 9.7% fat, and 9.2% moisture, and also contains dietary fiber and a small amount of minerals, so it is known as a nutritious food with functions such as anti-aging and skin beauty.

Carob is an evergreen tree of the legume family in the rose order of dicotyledons. It grows natively along the Mediterranean coast, and carob beans contain vitamins A, B, D, E, and minerals such as calcium and potassium, as well as essential amino acids and dietary fiber.

In the present invention, the okra, molokia, oat, wheat and carob bean mixed extract as an effective ingredient is preferably a mixture of a first extract obtained by immersing a mixture of okra, molokia, oat, wheat and carob bean in a solvent and extracting at a low temperature of 30 to 40°C, and a second extract obtained by fermenting the residue remaining after preparing the first extract with lactic acid bacteria and extracting it.

The above mixed extract can be prepared by the following method.

(A) a1) a step of immersing a mixture of okra, molokhia, oats, wheat and carob beans in at least one solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol and extracting at a low temperature of 30 to 40°C for 2 to 3 hours; a2) a step of filtering to prepare a first extract;

(B) b1) a step of recovering the okra, molokia, oat, wheat and carob bean residues remaining after preparing the extract in the above step (A); b2) a step of immersing the recovered residues in a solvent, inoculating them with lactic acid bacteria and fermenting them at 25-30°C for 22-26 hours; b3) a step of extracting the fermented product at 80-100°C for 2-3 hours; b4) a step of filtering to prepare a second extract; and

(C) It can be manufactured by a method including a step of mixing the first extract of step (A) and the second extract of step (B).

In order to efficiently manufacture the mixed extract of okra, molokia, oats, wheat and carob beans of the present invention, first, okra, molokia, oats, wheat and carob beans are mixed, and at least one solvent is selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol, and extracted at a low temperature of 30 to 40°C for 2 to 3 hours. The extract extracted by the above method is filtered to manufacture a first extract. (First extraction process)

At this time, preferably, the mixture is formed by mixing okra, molokhia, oats, wheat, and carob beans in a weight ratio of 1~2:1~2:1~2:1~2:1~2, respectively. When okra, molokhia, oats, wheat, and carob beans are mixed and extracted in a weight ratio of 2:1:1:1:1, respectively, it is more preferable because it exhibits better skin moisturizing, skin barrier strengthening, and skin texture improvement effects.

The above extraction process is carried out to efficiently extract polysaccharides. When extracted at high temperatures, a large amount of antioxidant components are extracted, but the polysaccharides are thermally decomposed, reducing the moisturizing effect. Therefore, the process is separated and carried out to increase the polysaccharide yield.

Next, a fermentation process is performed to efficiently obtain skin useful ingredients such as organic acids from okra, molokia, oats, wheat and carob beans. At this time, the raw material used in the first extraction process is recovered and reused. The residue remaining in the first extraction process is recovered, immersed in a solvent, and then inoculated with lactic acid bacteria and fermented at 25-30°C for 22-26 hours. The fermented product is extracted at a high temperature of 80-100°C for 2-3 hours. The solution is filtered to prepare a second extract. (Second extraction process)

At this time, the lactic acid bacteria used for fermentation is at least one selected from the group consisting of Lactobacillus plantarum, Lactobacillus lactis, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Pediococcus cerevisiae, Lactobacillus breve, Lactobacillus sakei, and Lactobacillus crispatus, and more preferably Lactobacillus plantarum.

In addition, at least one solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol, and butylene glycol is used as a solvent, preferably water.

Through the second extraction process including the above fermentation step, exfoliating and skin barrier strengthening ingredients can be efficiently extracted.

The first extract and the second extract prepared above are mixed in a weight ratio of 1 to 5:1 to 5, more preferably in a weight ratio of 1 to 2:1 to 2, to prepare a mixed extract of okra, molokia, oats, wheat, and carob beans.

The mixed extract of okra, molokia, oats, wheat and carob beans obtained by the extraction method including the above two-step extraction process exhibited a more excellent skin moisturizing effect (Test Examples 1, 2 and 4), skin texture improvement (Test Example 5) and skin barrier strengthening effect (Test Examples 3 and 6).

The mixed extracts of okra, molokia, oats, wheat and carob beans as active ingredients are contained in an amount of 0.01 to 50 wt% based on the total weight of the composition.

The above cosmetic composition can be used for moisturizing the skin, strengthening the skin barrier, or improving skin texture.

The cosmetic composition of the present invention can be manufactured in the form of general emulsified formulations and solubilized formulations. Cosmetics in the form of emulsified formulations include nourishing toners, creams, essences, etc., and cosmetics in the form of solubilized formulations include flexible toners. In addition, in addition to cosmetics, it can be manufactured in the form of an auxiliary agent that can be applied locally or systemically, which is commonly used in the field of dermatology, by containing a dermatologically acceptable medium and base.

In addition, the formulation of the cosmetic composition in the present invention is not limited, but may be applied as any one formulation selected from the group consisting of skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, pack, soap, shampoo, treatment, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, milk, pressed powder, loose powder, and eye shadow.

[Example]

Hereinafter, the present invention will be described in more detail based on the following examples and test examples. However, the following examples are only intended to illustrate the present invention, and the present invention is not limited by the following examples.

Comparative examples 1 to 3: Preparation of mixed extracts of okra, molokia, oats, wheat, and carob beans

Okra, molokia, oats, wheat and carob beans were mixed in the weight ratio shown in Table 1 below, immersed in 20 times the weight of purified water, extracted at a low temperature of 30°C for 2 hours, and then filtered to obtain the first extract, which is a filtrate. (First extraction process)

Comparative Examples 4-6: Preparation of mixed extracts of okra, molokia, oats, wheat and carob beans

The okra, molokia, oats, wheat and carob bean residues remaining in the first extraction process were recovered and immersed in 20 times the weight of purified water, inoculated with Lactobacillus plantarum, and fermented at 27°C for 24 hours. After fermentation, extraction was performed at 95°C for 2 hours. After cooling, the fermentation extract was obtained as the second extract by filtering. (Second extraction process)

division okra Molochia oats wheat Carob beans Comparative Example 1 1 1 1 1 1 Comparative Example 2 2 1 1 1 1 Comparative Example 3 1 2 1 1 1 Comparative Example 4 1 1 1 1 1 Comparative Example 5 2 1 1 1 1 Comparative Example 6 1 2 1 1 1

Examples 1-3: Preparation of mixed extracts of okra, molokia, oats, wheat and carob beans

The extracts prepared in Comparative Examples 2 and 5 were mixed in the weight ratio shown in Table 2 below to prepare a mixed extract of okra, molokhia, oats, wheat, and carob beans.

division Extract 1 Second extract Example 1 1 1 Example 2 1 2 Example 3 2 1

Test Example 1: Effect of moisturizing factor HAS-3 expression

Using Medium 106 medium supplemented with LSGS, fibroblasts (CCD-986Sk) were adjusted to 5× ¹⁰⁵ cells/well and seeded in a 12-well plate. After culturing the seeded medium for 18 hours, the samples of the examples and comparative examples of the present invention were treated at the same concentration and further cultured for 24 hours. RNA was extracted from the cultured cells, and the expression increase rate of HAS-3 (Hyaluronan Synthase-3) was confirmed through RT-PCR (Reverse transcription polymerase chain reaction). The expression increase rate of HAS-3 was calculated by the following formula, and the results are shown in Table 3 below.

HAS-3 expression rate (%) = (Sample HAS-3 expression amount / Control HAS-3 expression amount) × 100

Sample name Treatment concentration (w/v%) HAS-3 expression rate (%) Comparative Example 1 0.1 121.6 Comparative Example 2 0.1 124.1 Comparative Example 3 0.1 123.1 Comparative Example 4 0.1 105.2 Comparative Example 5 0.1 104.6 Comparative Example 6 0.1 106.4 Example 1 0.1 138.9 Example 2 0.1 134.4 Example 3 0.1 152.8 Hyaluronic Acid 0.05 163.9

As confirmed in Table 3 above, the sample of the example of the present invention, which is a mixture of the first extract and the second extract, exhibited a higher HAS-3 expression rate than the sample of the comparative example, which is each extract. Among them, Example 3 exhibited the best moisturizing effect.

Test Example 2: Measurement of hyaluronic acid production

The amount of hyaluronic acid produced was measured using the mixed extracts of okra, molokia, oat, wheat and carob beans manufactured in the comparative examples and examples above as samples. The test method is to evaluate the moisturizing ability by measuring the hyaluronic acid produced using HaCaT cells. First, HaCaT cells were dispensed at a concentration of 1× ¹⁰⁶ cells/well in a 6-well plate and cultured. After culture, the medium was replaced with one not containing FBS, starved for 18 hours, and additionally cultured for 24 hours. HaCaT cells were treated with each sample at the same concentration, and after culture for 24 hours, the culture fluid was collected to measure the amount of hyaluronic acid produced using an ELISA kit.

division Treatment concentration (%) HA production rate (%) Manufacturing example 1 1.0 34.8 Manufacturing example 2 1.0 35.2 Manufacturing example 3 1.0 37.2 Manufacturing example 4 1.0 14.1 Manufacturing example 5 1.0 12.5 Manufacturing example 6 1.0 18.5 Example 1 1.0 52.9 Example 2 1.0 44.7 Example 3 1.0 56.9 Hyaluronic Acid 1.0 58.5

As confirmed in Table 4 above, when the examples and comparative examples were treated at the same concentration, the sample of the example of the present invention showed a higher hyaluronic acid production rate.

Test Example 3: Effect of expression of skin barrier strengthening factor Filaggrin

Keratinocytes (HaCaT) were adjusted to 5 × 10 ⁵ cells/well using DMEM medium supplemented with 10% FBS, seeded in 12-well plates, and cultured for 18 hours, then treated with 200 mJ UVB and samples, and cultured for 24 hours. RNA was extracted from the cultured cells, and the expression increase rate of filaggrin (FLG) was confirmed through RT-PCR (Reverse transcription polymerase chain reaction). The expression increase rate was calculated by the following formula, and the medium was used instead of the sample for the negative control group, and retinoic acid was used as the positive control group. The results are shown in Table 5 below.

Barrier reinforcement rate (%) = (Sample FLG expression level / Negative control FLG expression level) × 100

Sample name Treatment concentration (w/v%) Barrier Reinforcement Rate (%) Comparative Example 1 0.1 121.6 Comparative Example 2 0.1 124.1 Comparative Example 3 0.1 123.1 Comparative Example 4 0.1 116.2 Comparative Example 5 0.1 110.6 Comparative Example 6 0.1 119.4 Example 1 0.1 146.9 Example 2 0.1 144.8 Example 3 0.1 152.4 Retinoic Acid 0.05 163.9

As confirmed in Table 5 above, when the comparative and example samples were treated at the same concentration, the sample of the example of the present invention showed a higher skin barrier strengthening rate. Among them, Example 3 showed the best skin barrier strengthening effect.

Example 4: Preparation of cream containing mixed extracts of okra, molokhia, oats, wheat and carob beans

A cream containing the mixed extract of Example 3 was prepared according to a conventional method with the composition and contents shown in Table 6 below.

Ingredients Content (weight%) Contrast example 1 Example 4 Mixed extract (Example 3) - 2.0 Monostearate glycerin 2.0 2.0 Stearyl alcohol 2.2 2.2 Stearic acid 1.5 1.5 beeswax 1.0 1.0 Polysorbate 60 1.5 1.5 Sorbitan stearate 0.6 0.6 purified vegetable oil 1.0 1.0 Squalane 3.0 3.0 Mineral oil 5.0 5.0 Trioctanoin 5.0 5.0 Dimethicone 1.0 1.0 Sodium magnesium silicate 0.1 0.1 glycerin 5.0 5.0 Betaine 3.0 3.0 Triethanolamine 1.0 1.0 Sodium hyaluronate 4.0 4.0 Preservatives, fragrances, colorings a very small amount a very small amount Purified water Remaining amount Remaining amount total 100 100

Test Example 4: Evaluation of skin moisturizing effect

The effect on skin moisturizing was evaluated using a skin moisture meter that measures skin conductivity. The skin moisture was measured under constant temperature and humidity conditions of 20 to 25℃ and 40 to 55% relative humidity, and the cream formulations of Example 4 and Control Example 1 were used. The cream formulations of Example 4 and Control Example 1 were applied twice a day to the faces of 10 subjects, and the moisture content before and after 2 weeks was compared. The results are shown in Table 7.

division Initial (D0) average 2 weeks (D14) average Moisture gain (%) Contrast example 1 30 34 13.4% Example 4 30 42 40%

As confirmed in Table 7 above, when the cream of Example 3 containing the mixed extract of the present invention was applied, a higher moisture increase was observed than that of Control Example 1.

Test Example 5: Evaluation of skin texture improvement effect

The skin texture improvement effect was confirmed using Dermatopblue (Breuck-Mann GmbH, Meersburg, Germany), which measures the degree of skin roughness. The faces of 10 subjects were divided in half, and the cream formulations of Example 4 and Control Example 1 were used respectively. The cream formulations of Example 4 and Control Example 1 were applied twice a day to the faces of 10 subjects, and the degree of skin roughness improvement before and after 6 weeks of use was compared. The results are shown in Table 8.

division Initial (D0) average 6 weeks (D42) average Skin texture improvement rate (%) Contrast example 1 100 96 4.0% Example 4 100 65 35.0%

As confirmed in Table 8 above, when the cream of Example 4 containing the mixed extract of the present invention was applied, a higher skin texture improvement rate was observed than that of Control Example 1.

Test Example 6: Effect of strengthening skin barrier function

In order to confirm the effect of strengthening the skin barrier function, the faces of 10 subjects were divided in half, and the cream formulations of Control Example 1 and Example 4 were applied twice a day for 4 weeks. After 4 weeks, the degree of improvement in the skin barrier function was evaluated using Delfin Vapo Meter SWL4255, which measures the amount of moisture loss in the transepidermal layer, and the results are shown in Table 9.

division Initial average (g/hm ² ) Average after 4 weeks (g/ ^hm2 ) Improvement rate (%) Contrast example 1 12.50 12.20 2.45 Example 4 12.50 8.00 36.0

As confirmed in Table 9 above, the formulation of Example 4 exhibited a high skin barrier function enhancement effect.

Claims (10)

A cosmetic composition containing a mixed extract of okra, molokia, oats, wheat and carob as an active ingredient, the mixed extract of okra, molokia, oats, wheat and carob being a mixture of a first extract obtained by immersing a mixture of okra, molokia, oats, wheat and carob in a solvent and extracting at a low temperature of 30 to 40°C and a second extract obtained by preparing the first extract and fermenting and extracting the remaining residue with lactic acid bacteria. delete A cosmetic composition according to claim 1, characterized in that the solvent is at least one selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol, and butylene glycol. A cosmetic composition in claim 1, characterized in that the lactic acid bacteria is at least one selected from the group consisting of Lactobacillus plantarum, Lactobacillus lactis, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Pediococcus cerevisiae, Lactobacillus breve, Lactobacillus sakei, and Lactobacillus crispatus. A cosmetic composition characterized in that in claim 1, the mixed extract is manufactured by extracting okra, molokhia, oats, wheat, and carob beans mixed in a weight ratio of 1 to 2:1 to 2:1 to 2:1 to 2:1 to 2:1 to 2, respectively. A cosmetic composition, characterized in that in claim 1, the mixed extract as an effective ingredient is contained in an amount of 0.01 to 50 wt% based on the total weight of the composition. A cosmetic composition characterized in that in claim 1, the first extract and the second extract are mixed in a weight ratio of 1 to 2:1 to 2, respectively. A cosmetic composition according to claim 1, characterized in that the cosmetic composition is for moisturizing the skin, strengthening the skin barrier, or improving skin texture. (A) a1) a step of immersing a mixture of okra, molokhia, oats, wheat and carob beans in at least one solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol and extracting at a low temperature of 30 to 40°C for 2 to 3 hours; a2) a step of filtering to prepare a first extract;
(B) b1) a step of recovering the remaining okra, molokia, oats, wheat and carob bean residues after preparing the extract in the above step (A); b2) a step of immersing the recovered residues in a solvent, inoculating them with lactic acid bacteria and fermenting them at 25-30°C for 22-26 hours; b3) a step of extracting at 80-100°C for 2-3 hours; b4) a step of filtering to prepare a second extract; and
(C) A method for producing a mixed extract of okra, molokia, oats, wheat and carob beans, comprising a step of mixing the first extract of step (A) and the second extract of step (B) obtained above in a weight ratio of 1 to 2:1 to 2, respectively. A method for producing a mixed extract of okra, molokia, oats, wheat, and carob, characterized in that in claim 9, the lactic acid bacteria is at least one selected from the group consisting of Lactobacillus plantarum, Lactobacillus lactis, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Pediococcus cerevisiae, Lactobacillus breve, Lactobacillus sakei, and Lactobacillus crispatus.