KR102647435B1 - Composition and method for manufacturing pepticide - Google Patents

Abstract

It relates to a method of producing a natural pesticide using plum ingredients, comprising: step S-1 of providing a plum ingredient material containing plum ingredients; Step S-2 of pulverizing the plum ingredient provided in step S-1 and then reacting with ethanol with a purity of 95% to 99.9%; and step S-3 of filtering the reaction solution reacted in step S-2. there is.

Description

Composition and manufacturing method of natural pesticide using plum reactant {COMPOSITION AND METHOD FOR MANUFACTURING PEPTICIDE}

The present invention relates to a composition and manufacturing method of a natural pesticide using plum reaction products. A pesticide composition that does not contain components harmful to the human body and does not contaminate the soil using only natural substances, and a pesticide composition using the composition to manufacture the pesticide. It's about how to do it.

The pesticides currently used are mainly manufactured through chemical methods, so they are harmful to the human body during the process of use or have a negative impact on the human body and the environment due to pesticides remaining in the environment after use.

Because of this problem, various eco-friendly natural pesticides have been released and used using natural substances containing insecticidal ingredients, but they are relatively less harmful to the human body and the environment than pesticides that use less chemical ingredients including natural substances or are chemically manufactured. In the sense that it is environmentally friendly in most cases, especially in the case of eco-friendly natural pesticides, their insecticidal power is generally insufficient compared to chemical pesticides, so they do not achieve sufficient insecticidal effect.

Existing pesticides or disinfectants include sodium hypochlorite, triethanolamine, fipronil, phtalthrin, permethrin, methylisothiazolinone, and phenoxyethanol. These substances cause rhinitis, asthma, headaches, vomiting, and tinnitus. It often causes abnormal symptoms by irritating the skin and lungs, so caution is required when using it, and it must be used with extreme caution when using it on humans or animals.

In relation to this, in an effort to manufacture pesticides using plum, there have been cases where inventions using plum extract mixed with other insecticidal ingredients, such as Korea Patent Registration No. 10-152431 and Korea Patent Registration No. 10-0471111, have been disclosed, but plum Rather than saying that an effective insecticide was developed using its insecticidal power, this is a case of manufacturing an insecticide by simply adding plums.

In particular, conventional technologies estimate the insecticidal effect of various organic acids such as amygdalin, amygdalin-derived hydrogen cyanide, ethyl carbamate, citric acid, formic acid, and oxalic acid contained in plums. However, as a result of various experiments, the actual insecticidal power of these substances is not confirmed. It is evaluated to be at a minimal level compared to the insecticidal power of the present invention, and in the case of the present invention, the insecticidal effect is not due to ingredients known or presumed to have insecticidal action in the past, but it is easily extracted with high-purity alcohol and volatility when boiled. Considering the ingredients it contains, it can be confirmed that it has an insecticidal effect due to an insecticidal ingredient that is completely different from the conventional insecticidal ingredients mentioned above.

The present invention was developed to overcome the problems described above, and its purpose is to replace existing pesticides using chemicals harmful to the human body by obtaining an insecticidal effect using natural substances that are harmless to the human body.

In order to achieve the above-mentioned purpose, a method for producing a natural pesticide using plum ingredients is provided, which includes step S-1 of providing a plum ingredient material containing plum ingredients; Step S-2 of pulverizing the plum ingredient provided in step S-1 and reacting it with ethanol with a purity of 95% to 99.9%; It includes step S-3 of filtering the reaction solution reacted in step S-2.

In step S-2, 250 to 300 parts by weight of ethanol may be reacted for 24 to 72 hours with respect to 100 parts by weight of plum ingredient material.

Step S-3 can be performed by diluting water into the reaction solution.

After step S-2, water is added to the reaction solution to cause an exothermic reaction in the reaction solution. 120 to 150 parts by weight of water can be added to 100 parts by weight of the reaction solution prepared in step S-2.

It further includes the step of adding ethanol and water to the residue remaining from the plum ingredient material reacted in step S-2 (step S-4), wherein 120 to 150 parts by weight of a mixture of ethanol and water is added to 100 parts by weight of the plum essence ingredient residue. The content of ethanol in the mixture of ethanol and water may be 20 to 30% by weight.

The plum ingredient material may be any one of the group consisting of plum pulp, plum leaves, plum wine residue, plum liquid residue, and the group consisting of dried products thereof.

By processing and dissolving plums with ethanol and water, unlike existing pesticides, pests are exterminated using completely natural products or substances derived from natural products, so they do not cause problems even when directly exposed to the human body, are not harmful to the environment, and do not cause damage to crops. Not only does it not cause any harm, but it also appears to promote the growth of crops.

Using this, people who grow crops in crowded areas such as cities can use it with peace of mind, and they can eat fruits and vegetables with confidence as they are not exposed to chemicals such as pesticides. And through the effect of promoting plant growth, it is possible to achieve both an insecticidal effect and an increase in yield.

also. Since the processed liquid prepared in the present invention has a common effect on insects, it can be used to kill dust mites not only on crops but also on bedding, and can also be used to kill insects that live on the fur and skin of pets. In addition, it has an itching-relieving effect, so it can be used as an auxiliary treatment for atopic dermatitis, and its sterilizing effect is as strong as that of other plum preparations, so it has been used as a hand sanitizer and mask sterilizer during the coronavirus outbreak.

Additionally, since the residue remaining after manufacturing pesticides can be used as compost, no waste is generated during the manufacturing process of pesticides, thereby freeing the environment from pollution.

1 is a diagram showing the manufacturing process according to the present invention.
Figure 2 is a diagram showing the results of an experiment on the toxic effect of the pesticide according to the present invention.

Hereinafter, the present invention will be described in detail with reference to the attached drawings. The active ingredient of the pesticide composition of the present invention is derived from plum flesh, plum seeds, plum leaves, plum wine residue, plum juice residue, etc., and kills pests that harm crops, various trees, grass, etc., but is a natural substance that is harmless to plants and the human body. It is an ingredient. Not only can insecticidal ingredients be extracted from plum pulp and plum leaves collected from plum trees, but pesticides can also be manufactured using plum residue left after plum wine and plum residue left after plum syrup.

The pesticide according to the present invention can be extracted from plum flesh, plum leaves, plum seeds, etc., and for this purpose, the plum flesh, etc. is separated and then extracted using ethanol. Since plums are mainly harvested in early June, the harvested plums can be dried and stored for use. .

There are many substances contained in plums. Since ancient times, plum wine and plum tea have been known to have strong sterilizing and detoxifying effects and have been widely used to prevent and treat food poisoning and to treat constipation and diarrhea. However, there is controversy over its harmfulness because it contains small amounts of amygdalin and ethyl carbamate, which are converted to prussic acid, a toxic substance, through an enzymatic reaction in the human body. However, in the present invention, it was confirmed through experiments that amygdalin and ethyl carbamate are not the main mechanism of insecticidal action.

Additionally, in the case of natural extracts, it is very difficult to pinpoint the active ingredients.

Plums contain a large amount of various types of organic acids, most of which are citric acid, malic acid, and oxalic acid, and small amounts of picric acid, pruna acid, ursolic acid, and pyraminic acid.

go). citric acid

Citric acid is the component that accounts for the largest proportion of the organic acids in plums and is used as an acid seasoning for soft drinks, mixed liquor, candy, jelly, jam, frozen confectionery, canned food, etc., and as a rancidity prevention agent for cooking oil. It is known to be non-hazardous. The boiling point is 175 ㅀC and it is highly soluble in water and alcohol.

me). malic acid

Malic acid is the second most abundant organic acid contained in plums and is used as a flavoring agent in various beverages. It has a boiling point of 127 ㅀC and is highly soluble in water but insoluble in alcohol.

all). oxalic acid

Oxalic acid is the third largest component among the organic acids contained in plums and is a toxic substance that is harmful to the kidneys and is known to cause urinary stones by reacting with calcium ions as shown in the chemical formula below. It decomposes at 189.5 ㅀC and is highly soluble in water and alcohol. The boiling point is 255 ㅀC and it is insoluble in alcohol. Additionally, oxalic acid has the property of being neutralized into calcium oxalate when it encounters calcium. Oxalic acid is used as a raw material for bleach and is known to have a skin whitening effect.

la). picric acid

Picric acid is a component contained in trace amounts in plums and is known to have the effect of decomposing toxins and detoxifying the liver. It is also known to be used as a raw material for explosives along with TNT and as a raw material for pesticides.

mind). Prunasin

Prunasin (C ₁₄ H ₁₇ NO ₆ ) is a component contained in trace amounts in plums. It produces hydrogen cyanide (HCN, prussic acid) through hydrolysis and is highly soluble in water and alcohol.

bar). Catechinic acid

Catechinic acid is a well-known ingredient that gives green tea its bitter taste, and is said to have sterilizing and detoxifying effects, so it is used to prevent food poisoning. In the case of plum trees, it is known to be abundant in plum leaves, and its boiling point is around 630 ㅀC and has low solubility in water. In particular, catechin acid, which produces a bitter taste, is known to decompose and decrease when roasted.

buy). Dermal acid

Pyric acid is an ingredient contained in trace amounts in plums and is known to have a sterilizing and detoxifying effect, preventing food poisoning. The boiling point is 255 ㅀC, and it has low solubility in water.

ah). Amygdalin

Amygdalin is a component that is contained in trace amounts not only in plum seeds, but also in plum flesh and plum leaves. It is known to be toxic when converted to hydrogen cyanide (cyanide, cyanide) through an enzymatic reaction in the human body, so there is controversy regarding its harmfulness. .

Amygdalin decomposes at temperatures above 70 ㅀC and is highly soluble in water and alcohol.

Hydrogen cyanide is a colorless, highly toxic compound whose lethal dose is known to be about 2 g. It is highly volatile, boils at 26°C, slightly higher than room temperature, has a bitter taste, has a unique odor, and is slightly acidic.

It is known that the content of amygdalin can be adjusted depending on the ripening state of the plum or the method of storing plum wine and plum juice. It is known that the plum harvest time is delayed or the plums are removed from the plum wine and plum juice 100 days before the amygdalin content is maximized. However, it has recently been reported that if the ripening period of plum wine and plum juice is prolonged, amygdalin almost disappears.

ruler). ethyl carbamate

The hazards of ethyl carbamate, a 2A carcinogen, are more highlighted than the hazards of amygdalin. As shown below, hydrogen cyanide (cyanic acid, cyanide) produced by amygdalin or prunasin produces cyanic acid (cyanate), which is converted into ethanol. When reacted with , ethyl carbamate is produced.

HCN(Cyanide) → HOCN(Cyanate) + Ethanol → Ethyl carbamate (C ₃ H ₇ NO ₂ )

Ethyl carbamate boils at 185 ㅀC and is highly soluble in water. It is known that in the process of making plum wine and plum juice, more ethyl carbamate is produced the longer the leaching storage time, the stronger the light, the higher the temperature, and the higher the alcohol concentration. .

car). potassium hydroxide

When the potassium contained in plums dissolves and produces potassium hydroxide, it can react with hydrogen cyanide to form highly toxic potassium cyanide, known as cyanide, as shown in the chemical equation below.

HCN + KOH → KCN + H ₂ O

Potassium cyanide has a boiling point of 1625 ㅀC and can be detoxified with hydrogen peroxide as shown in the chemical equation below.

KCN + H ₂ O ₂ → KOCN + H ₂ O

Some of the substances mentioned above have been confirmed to have insecticidal properties, but there is nothing known about the increase in insecticidal power when secondary extraction is performed by adding water after primary extraction.

Figure 1 shows a method for producing an insecticide according to the invention. The numbers shown in the drawing indicate the number of each step.

Step 1: Plum by-products (plums, plum leaves, plum wine residue, plum juice residue, etc.) used in the production of pesticides are prepared by crushing them as is, drying and then crushing, ripening and then crushing, or burning and then crushing.

Step 2: This is the process of reacting the plum by-product obtained in Step 1 by soaking it in ethanol. The reaction time is sufficient for the reaction solution to turn dark green and 24 to 72 hours is sufficient. This process is called the first reaction process. .

The ethanol used is a high-purity product with a purity of 90% or more and 99.9% or less (preferably 95% or more, mass ratio). In the case of plums, approximately 650g of pulp is obtained after removing the seeds from 1kg of plums. After pulverizing the plum flesh, the purity is approx. Add 1.8 liters of 95% ethanol and react for about 48 hours, then add 3.3 liters of water again for a secondary reaction. At this time, you can see that the moment water is added in the secondary reaction, an exothermic reaction occurs.

Tap water uses a large amount of chemicals to sterilize various harmful substances or remove green algae, and these chemicals clearly show a decrease in insecticidal power. Therefore, when making pesticides, bottled water or purified water is used, or groundwater that has passed a water quality test is used. It's good to use.

Step 3: After the first reaction process is completed, filter the reaction solution and mix it with water.

Step 4: After the first reaction process is completed, the filtered solution is diluted with water to make pesticide A.

Step 5: After the primary reaction process is completed, pesticide B is prepared by adding water to the reaction solution and performing a secondary reaction without filtering it. It is effective to adjust the ethanol content in the reaction solution to a range of 15 to 25% by weight relative to the weight of the total solution.

Step 6: Filter the secondary reaction solution.

Step 7: This is the process of making pesticide B using the filtered secondary reaction solution. It can be used by diluting it in water if necessary.

Step 8: In order to produce pesticide C, a secondary reaction is carried out by adding ethanol and water simultaneously to the residue from which the reactants generated in the primary reaction process have been removed. The ethanol content is 15~15% by weight based on the weight of the total reaction solution. It is effective to adjust it to 25% range.

Step 9: This is the process of filtering the secondary reaction solution.

Step 10: This is the process of making pesticide C using the filtered secondary reaction solution. It can be used by diluting it in water if necessary.

Step 11: This is the process of producing pesticide D by simultaneously adding ethanol and water to the plum by-product prepared in step 1.

Step 12: Filtering the reaction solution

Step 13: This is the process of making pesticide D using the filtered reaction solution. If necessary, it can be diluted in water and used.

Specifically, the natural pesticide according to the present invention can be produced by the following method. In the case of natural pesticides, it is very difficult to determine the main active ingredient, but the manufacturing method has the advantage of being relatively easy.

Example 1: Example of pesticide preparation method

1-1. Plum pulp, plum leaves, plum seed extract

Only the raw plum flesh was separated from the plum seeds and crushed, or the crushed raw plum flesh was dried and mixed with ethanol and water. In the same way, the plum leaves and plum seeds were crushed as is, roasted and then pulverized, or dried and then pulverized, and added to the reaction vessel with ethanol and water. Water was added to cause the reaction. If you have plum pulp available, you do not need to dry it separately and can use it as is. If you only have pulp, you can use only the pulp. If you have plum leaves or seeds, you can use only plum leaves or plum seeds. Ethanol with a purity of 95% or higher was used. In general, the higher the purity of ethanol used, the greater the insecticidal power, and it is okay to use ethanol with a purity of 95% or less.

Insecticidal power can also be obtained when preparing a mixed solution using ethanol and water together (insecticide D in Figure 1), but the insecticidal power becomes stronger if water is added to the mixed solution after the primary reaction with ethanol (insecticide D in Figure 1). A).

In particular, it can be used when the insecticidal power is maximized and a strong insecticidal effect is required when adding water after the primary reaction for a secondary reaction (insecticide B in Figure 1) or removing the mixed solution and performing a secondary reaction with ethanol and water. (insecticide C in Figure 1).

More in-depth research is needed as to why the insecticidal power becomes stronger after a secondary reaction using water, but when water is added after reacting with pure ethanol, heat is instantly generated and the temperature rises, a new reaction including hydrolysis reaction. This seems to happen, and experimentally, the insecticidal effect is experimentally superior to reacting with ethanol first and then adding water, rather than reacting with ethanol and water together.

Previously, amygdalin or ethyl carbide, which are well known among plum ingredients, were extracted and used, but it appears that it is not amygdalin or ethyl carbite that acts as an insecticide in the present invention. In addition, the insecticidal power increases simply by adding water extracted after the first reaction, and this is not a reaction that occurs with previously known ingredients, which will be described later.

To increase the efficiency of pesticide manufacturing, for example, after completing the primary reaction with high-purity ethanol for 48 hours, ethanol and water are added to perform the secondary reaction for 72 hours, and then the residue is filtered out and used as a pesticide.

For 650g of plum flesh, add 1.8 liters of 95% purity ethanol and react for 48 hours, then add 3.3 liters of water and react for 72 hours, or after 48 hours of reaction, add 1.8 liters of ethanol again and react for 72 hours and filter out the residue. It can be used as an insecticide.

1-2. Reaction solution using plum residue remaining after containing plum wine

The pulp and seeds were separated from the plum residue remaining after containing plum wine and crushed, or the crushed pulp was dried and then reacted with ethanol and water.

As in Example 1-1, insecticidal power can also be obtained if a reaction solution is prepared in a reaction vessel using ethanol and water at the same time (insecticide D in Figure 1), but water is added to the reaction solution after the first reaction with ethanol. When a hydrolysis reaction occurs, the insecticidal power becomes stronger (insecticide A in Figure 1).

In particular, it can be used when the insecticidal power is maximized and a strong insecticidal effect is required when performing a secondary reaction by adding water after the primary reaction (insecticide B in Figure 1) or removing the reaction solution and performing a secondary reaction with ethanol and water. (Pesticide C in Figure 1).

To increase the manufacturing efficiency of pesticides, for example, after completing the primary reaction with high-purity ethanol for 48 hours, ethanol and water are added to perform the secondary reaction for 72 hours, and then the residues can be filtered out and used as pesticides.

1-3. Reaction solution using plum juice or residue remaining after containing plum juice

Separate the pulp and seeds from the plum juice or plum residue remaining and grind them, or dry the crushed pulp and react it with ethanol and water.

In this case, insecticidal power can also be obtained if the reaction solution is prepared in a reaction vessel using ethanol and water simultaneously. When the reaction was carried out by adding water to the reaction vessel, the insecticidal power was further increased.

However, the sugar remaining in the plum juice residue had the opposite effect of attracting ants, and when used as an insecticide, care must be taken to avoid attracting ants and insects because it is natural, but in addition, plum juice and plum juice residue have a negative effect on attracting ants. The effect can be doubled as the residual sugar can be used to attract and kill cockroaches and slugs using the attractant effect and insecticidal ingredients.

Example 2: Evaluation of insecticidal power using reactants

In order to confirm the insecticidal power of the reactant, cockroaches, mites, gnats, cutworm larvae, scale insects, etc. were collected, the reactant was sprayed, and the insecticidal power was measured by the time it took for them to die. Insecticidal power is calculated by the following formula.

Insecticidal activity (%) = [Number of dead insects/(Number of live insects + Number of dead insects)]ㅧ100

Table 1 shows an example of pesticidal power evaluation in Example 1-2 when the primary reaction solution is removed and reacted with ethanol and water again.

In Example 1-2, when the primary reaction solution was removed and the remaining by-products were reacted again with ethanol and water before use, in the case of cockroaches, when spraying insecticide, they died instantly within a few seconds or all individuals died within 1 hour. All aphids died within 1 hour. In the case of larvae of larvae and cutworm moths, they were not completely killed within 1 hour, but all individuals were killed within 1 day. Through these results, it was confirmed that all pests did not die immediately when hit with the reaction solution, but all individuals died within 1 day.

The insecticide according to the present invention also has an insecticidal effect on insects other than those shown in Table 1. Although there are differences in insecticidal power, insects that have shown insecticidal effects include slugs, flies, money beetles, millipedes, and various butterfly and moth larvae. In particular, with the exception of some species, ladybugs are beneficial insects that eat aphids. As a result of testing with this pesticide, it was found that the insecticidal effect on ladybugs was weak, which seems to provide synergy in pest control.

Example 3: Safety evaluation

3-1. Crop growth impact assessment

In order to check whether the growth of crops was inhibited, the reaction solution was sprayed repeatedly for 3 days to ensure that the target crops were soaked, and then it was confirmed whether there were any problems with the growth of the crops. At this time, the side that was not sprayed with the reaction solution and the side that was sprayed with the pesticide were designated as control group 1 and control 2, respectively, and the side that was sprayed with the plum reaction solution was treated as the experimental group, and changes in the crops were observed for 15 days. Table 2 shows an example of crop growth impact assessment results.

When comparing crops sprayed with pesticides or plum extracts and crops sprayed with nothing (Table 2), unlike Control 1, which suffered damage such as holes or wilting in leaves due to larvae or mites, Control 2 and the experimental group Growth conditions, leaf conditions, etc. showed good or excellent results.

In particular, it can be observed that growth is promoted in the experimental group compared to control group 2, which used pesticides.

Through this, we can see that plum extract has the effect of eradicating pests like pesticides and at least does not hinder the growth of plants or worsen their condition. In particular, considering that the pesticide according to the present invention is a natural ingredient, it can be said to be very excellent in terms of safety because no damage is caused by pesticides.

3-2. Fish toxicity evaluation

To check toxicity to fish, 11 goldfish and 11 guppies each measuring 2 cm were placed in a separate fish tank under the condition of administering 100 mg of the reaction solution per liter of water, mixed with the insecticidal reaction solution, and kept for 4 days (96 hours). Observation was conducted to determine the number of dead individuals (when no response or breathing occurred when stimulated) every 24 hours.

The results of evaluating toxicity to fish showed that both goldfish and guppies survived, as shown in Figure 2. Through this, it was also revealed that the pesticide in question is a harmless ingredient that does no harm to fish in the river.

Figure 2 shows these results, and it can be seen that there is no effect on the living environment of fish.

4. Insecticide ingredients

In the present invention, the substance used for insecticide comes from plum ingredients, and a method of processing the substance containing plum ingredients is presented so that it can be used as an insecticide. In the present invention, the substance that acts on insecticides is amygdalin, which decomposes above 70°C because its strong insecticidal power is confirmed even when extracted with ethanol using burnt plums and its strong insecticidal power is confirmed even when volatile substances are volatilized at 80°C. It can be confirmed that the insecticidal action is not caused by substances produced by amygdalin-derived hydrogen cyanide, and ethyl carbamate and ethyl carbamate boiling at 185°C.

In addition, when the reactant is boiled at 100°C, the insecticidal power is weakened or eliminated. Since the insecticidal substance according to the present invention is volatile between 80 and 100°C, various organic acids contained in plums such as citric acid, oxalic acid, and formic acid are added. It can be confirmed that it does not cause insecticidal action.

In addition, unlike the conventional report that the insecticidal action occurs because the plum extract is acidic, the pH and insecticidal power of the insecticidal substance that exerts the insecticidal action in the present invention are shown to be unrelated.

In addition, when water is added after reacting with high-purity ethanol, heat is temporarily generated, and a more powerful insecticidal effect can be obtained than when ethanol and water are used at the same time or when low-proof ethanol is used.

What can be seen from the above experimental results is that the main component that causes insecticidal action is a component that reacted with ethanol and is newly produced by reacting with water.

This is not only a different method from the conventional method of killing insects by adding plum extract, but also shows that the insecticidal action is caused by a different substance than the previously reported insecticidal substances.

Table 4 shows the characteristics of the insecticidal substances that cause insecticidal effects in the present invention.

Amygdalin and ethyl carbamate were not detected through GC/MSD analysis results, and trace amounts of cyanate and hydrogen cyanide were detected, but as they are substances with a low boiling point of less than 30 degrees Celsius, they are unsuitable to produce a major insecticidal effect in plum reactants. There is not enough quantity.

Ethylene detected in plum extract is a substance that promotes plant growth and is interpreted as the cause of accelerated growth compared to the case where pesticides are not used or chemical pesticides are used. The manufacturing method to maximize insecticidal power is shown in Figure 1. When reacted primarily with ethanol and then additionally added with a mixture of ethanol and water, the insecticidal power is stronger than when reacted with ethanol alone or a mixture of ethanol and water. Although the rebound solution prepared in the present invention strongly kills cockroaches, aphids, scale insects, etc., it does not harm crops. In addition, through acute toxicity tests using goldfish and guppies, it can be confirmed that it is an eco-friendly pesticide that is harmless to fish in rivers.

Since the substance according to the present invention is a natural substance, it can be used as an insecticide or disinfectant. Compared to existing hand sanitizers, it is non-irritating and non-volatile, so it does not cause any irritation to the eyes unless intentionally put into the eyes, and is a chlorine-based substance harmful to the human body. No additives included.

Additionally, by adjusting the concentration, it can be used to relieve itching caused by skin diseases in livestock such as chickens or pet dogs, and to sterilize ticks or bacteria after an outdoor walk.

Claims (6)

A method of producing a natural pesticide using plum ingredients, comprising: step S-1 of providing a plum ingredient material containing plum ingredients;
Step S-2 of pulverizing the plum ingredient provided in step S-1 and reacting it with ethanol with a purity of 95% to 99.9%;
It includes the S-3 step of filtering the reaction solution reacted in the S-2 step.
In step S-2, 250 to 300 parts by weight of ethanol is reacted for 24 to 72 hours with respect to 100 parts by weight of plum ingredient material.
A method of producing a natural pesticide in which water is added to the reaction solution after step S-2 to cause an exothermic reaction in the reaction solution, and 120 to 150 parts by weight of water is added to 100 parts by weight of the reaction solution made in step S-2.
delete The method of claim 1, wherein in step S-3, water is diluted into the reaction solution. delete The method of claim 1, further comprising adding ethanol and water to the residue remaining from the plum ingredient material reacted in step S-2 (step S-4), wherein ethanol and water are added to 100 parts by weight of the plum ingredient residue. A method of producing a natural pesticide using plum ingredients, wherein 120 to 150 parts by weight of the mixture is mixed and the ethanol content in the mixture of ethanol and water is 20 to 30% by weight. The method according to any one of claims 1, 3, and 5, wherein the plum ingredient is any one of the group consisting of plum flesh, plum leaves, plum wine residue, plum liquid residue, and the group consisting of dried products thereof. How to manufacture natural pesticides using plum ingredients