KR102531063B1 - Complex microbial fermentation broth for livestock gas removal and livestock health promotion - Google Patents

Abstract

Provided is a complex microbial fermentation solution for removing livestock gases and improving livestock health, which contains Bacillus subtilis, Bacillus Licheniformis, Lactobacillus, photosynthetic bacteria, and complex vitamins.

Description

Complex microbial fermentation broth for livestock gas removal and livestock health promotion}

The present invention relates to a complex microbial fermentation broth for removing livestock gas and promoting livestock health.

The livestock industry is one of the fields of agriculture that raises livestock to supply meat and by-products, and is an important key industry in Korea. However, as the quality of life increases and interest in the environment increases, concerns about odors generated during livestock farming and their side effects are increasing, and accordingly, interest in methods for removing odors is increasing. Odors generated in livestock sheds are mainly generated in the process of microorganisms decomposing various nutritional components in livestock manure to be composted or in the process of decomposing food by microorganisms in the intestines of livestock that have consumed the food. Livestock feces contain a large amount of organic substances and various nutrients that can be used as energy sources for microorganisms, and odors are caused by various gases and volatile substances generated when they are decomposed by microorganisms in an anaerobic environment.

This livestock odor causes a drastic decrease in livestock productivity, an increase in mortality, and a decrease in feed efficiency. In addition, it causes civil complaints around the barn as a cause of farmland pollution, river pollution, and air pollution. Livestock odors are mostly caused by feces and excreta, which are organic substances that have been enzymatically decomposed by metabolic principles. It is caused by very high concentrations of organic matter such as

In addition, suppression of the growth of beneficial bacteria, inhibition of the growth of active factors of natural microorganisms, and inhibition of the activity of fermentation strains due to the abuse of antibiotics also cause an increase in the odor of fatty acid volatile odorants (VFC). Odor substances include ammonia, methyl mercaptan, H ₂ S, sulfic methyl, disulfic methyl, trimethylamine, CH ₃ COOH, and styrene. Representative substances are ammonia and amines, which account for 95% of livestock odor. occupy Ammonia is primarily irritating enough to sting the eyes, and has a great correlation with the occurrence of respiratory diseases in particular. In addition, when exposed chronically, it acts as a major stress factor for pigs, which adversely affects the growth and health of young pigs. According to the results of several studies so far, ammonia reduces the growth rate of piglets, there is a very close correlation between the ammonia concentration in the pig house and the incidence of respiratory diseases and porcine stress syndrome, and the ammonia concentration in the pig house increases. It has been reported that the incidence and mortality of these diseases increase with As such, the ammonia concentration in the pig house causes a direct economic loss by reducing the growth rate of pigs as well as increasing the mortality rate, and the increase in ammonia concentration increases the occurrence of respiratory diseases, causing additional drug costs.

To reduce livestock odor, general animal medicines and feed additives are used. Odor-reducing chemicals include chloric acid (ClO ₂ )-based agents (caustic soda mixtures), powdered enzymes, plant extracts and photosynthetic mixtures, bioactive agents, probiotic mixtures, oxidizing agents, acid-base neutralizing agents, and masking agents. However, it is very insufficient to remove the odor of livestock and feces, which are in a continuous state of ammonia and amines. In particular, alkali agents rather amplify odor induction, neutralizers have negligible removal of odorous substances, and microbial agents are difficult to expect effects without environmental composition such as growth conditions and temperature of microorganisms. In addition, many feed additives, supplementary feeds, single feeds, functional feeds, probiotics, etc., which are said to reduce livestock odor, have been developed and supplied, but the effect is very insignificant.

Korean Patent Registration No. 1212031

The present invention decomposes and removes odorous gases such as carbonic acid, nitrogen, and sulfur compounds, which are odorous gases generated in the intestines of livestock or manure, and quickly decomposes and removes harmful organic acids such as acetic acid, propionic acid, butyric acid, and valeric acid in livestock sheds. It suppresses the occurrence of livestock diseases and reduces stress by removing unpleasant and highly toxic secondary amine compounds, hydrogen sulfides, and mercaptans, and has the ability to decompose and remove ammonia components and volatile organic substances. It is excellent in minimizing the generation of odor, proliferating beneficial microorganisms such as lactic acid bacteria and bacillus in the intestines of livestock to remove pathogenic microorganisms and reduce odor as well, and has antiviral and antibacterial active substances to inhibit the growth of various pathogens. Prevents infectious diseases, eliminates clogging of manure with excellent ability to decompose high-concentration organic matter, provides cost savings for wastewater treatment with easy treatment of high-concentration manure, and maintains a pleasant barn environment by suppressing the occurrence of pests such as flies and mosquitoes. It is an object of the present invention to provide a complex microbial fermentation broth for removing livestock gases and promoting livestock health, which acts as a major microorganism in liquefied fertilizer fermentation and has an excellent fertilizer effect by its fermentation product.

In order to achieve the above object, the present invention

Bacillus subtilis ( Bacillus subtilis ), Bacillus licheniformis ( Bacillus Licheniformis ), lactic acid bacteria ( Lactobacillus ), photosynthetic bacteria, and complex vitamins for removing livestock gas and promoting livestock health are provided.

The complex microbial fermented broth for livestock gas removal and livestock health promotion of the present invention decomposes and removes odorous gases such as carbonic acid, nitrogen, and sulfur compounds, which are odorous gases generated in the intestines of livestock or manure, and acetic acid, propionic acid, and lactic acid in the livestock barn. It rapidly decomposes and removes harmful organic acids such as valerian acid, and suppresses the occurrence of livestock diseases and reduces stress by removing unpleasant and highly toxic secondary amine compounds, hydrogen sulfides, and mercaptans, and ammonia components. and excellent ability to decompose and remove volatile organic substances, thereby minimizing the occurrence of odor, and by proliferating beneficial microorganisms such as lactic acid bacteria and bacillus in the intestines of livestock, removing pathogenic microorganisms and reducing odor as well, and antiviral and antibacterial active substances It inhibits the growth of various pathogens to prevent various infectious diseases, has an excellent ability to decompose high-concentration organic substances, relieves manure clogging, and provides an effect of reducing wastewater treatment costs because it is easy to treat high-concentration manure. It suppresses the occurrence of pests to maintain a pleasant barn environment, and acts as a major microorganism of liquid manure fermentation to provide excellent fertilizer effect by the fermentation product.

The complex microbial fermentation broth for removing livestock gas and promoting livestock health of the present invention is characterized by including Bacillus subtilis , Bacillus Licheniformis , Lactobacillus , photosynthetic bacteria, and complex vitamins.

The bacillus is an aerobic or facultative anaerobic bacterium and widely exists in soil and aquatic environments. Bacillus is an industrially very important strain used in the production of various enzymes, vitamins, antibiotics, and insecticides. It rapidly solubilizes and decomposes high-molecular substances such as starch, protein, cellulose, fat, and xylan, and magnetizes odorous components. It can greatly increase digestibility and growth rate.

As the Bacillus subtilis and Bacillus Licheniformis, strains known in the art may be used. For example, as Bacillus subtilis, KCTC 3560 strain, KCCM11143P strain, R2-1 (accession number: KACC91542P) strain, Bs5C (accession number: KCTC 18585P), etc. may be used, and as Bacillus licheniformis JLRI 05 (accession number: KCCM12183P) strain, Bacillus licheniformis AEY-3 (accession number: KCTC18316P) strain, etc. may be used.

The lactic acid bacteria are one of the most beneficial microorganisms available to humans and are widely distributed in the natural world, such as the digestive tract of mammals, various fermented foods and soil. Various organic acids (lactic acid, acetic acid, benzoic acid) produced by these lactic acid bacteria neutralize pathogenic bacteria by acidifying the intestinal pH, and restore the intestinal flora balance to normal to prevent diarrhea and enteritis. In addition, hydrogen peroxide produced by lactic acid bacteria kills pathogens by strong oxidative action on cells and destruction of protein structure, and bacteriocin, an antibiotic produced by lactic acid bacteria, inhibits the growth of decay product-producing bacteria. Therefore, lactic acid bacteria maintain normal bacterial flora in the intestine, suppress pathogenic microorganisms, and in particular, provide an effect of improving the growth rate and feed efficiency of animals by preventing enteritis and Hari.

As the lactic acid bacteria, at least one selected from the group consisting of Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus casei, and Lactobacillus reuteri may be used, and strains known in the art are limited as these strains. can be used without For example, the strain deposited as KCTC 3164 may be used as the Lactobacillus acidophilus, the strain deposited as KCCM12355P may be used as the Lactobacillus brevis, and the strain deposited as KCTC 2180 may be used as the Lactobacillus casei. may be used, and a strain deposited as KCCM 10651P may be used as the Lactobacillus reuteri.

Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus casei, and Lactobacillus reuteri may be included in the form of a culture medium having a volume ratio of 1 to 1.5: 1 to 1.5: 1 to 1.5: 1 to 1.5, respectively. The culture medium preferably has a viable cell count of 1X10 ⁵ to 1X10 ⁸ cfu/ml.

The photosynthetic bacteria are bacteria that perform carbon assimilation using light energy, such as higher plants, and include red non-sulfur bacteria, red sulfur bacteria, and green sulfur bacteria. Photosynthetic bacteria are Gram-negative bacteria, 65% of which are protein, and contain a high content of essential amino acids and vitamins. When used as feed, the survival rate, spawning rate, and digestibility are improved, and hydrogen sulfide and organic acids, which cause odor, are removed. Because it is used, it is also effective in removing odors in livestock sheds.

Bacteria known in the art may be used as the photosynthetic bacteria in the present invention, and for example, Rhodopseudomonas capsulata, particularly Rhodopseudomonas capsurata KCTC 2583 may be used.

In the present invention, the multivitamin includes vitamin A, vitamin D, vitamin E, vitamin B3, and vitamin B7 in a weight ratio of 0.8 to 0.9: 0.001 to 0.005: 0.001 to 0.003: 0.08 to 0.15: 0.01 to 0.06. The multivitamin provides effects such as feed intake rate, digestion promotion, formality improvement, fecal odor reduction, and anti-pathogenicity enhancement.

In the complex microbial fermentation broth of the present invention, the Bacillus subtilis , Bacillus Licheniformis , Lactobacillus , photosynthetic bacteria, and complex vitamins are 1 to 1.5: 1 to 1.5: 1 to 1.5, respectively. : 1 to 1.5: has a weight ratio of 1 to 1.5, and the Bacillus subtilis, Bacillus Licheniformis, Lactobacillus, and photosynthetic bacteria are 1X10 ⁵ ~ 1X10 ⁸ cfu / ml It may be a culture medium having a viable cell count.

The complex microbial fermentation broth of the present invention may be prepared by mixing the above strains and directly using them, or by mixing and further culturing them.

In the case of further culturing in the above, sugar components are added to water, and after the components are added, the lid is well covered and sealed, and the temperature is maintained at a place where the temperature is maintained at 25° C. or higher and 30° C. or lower. Since gas is generated during the fermentation process, it is used after culturing for 3 to 4 days while removing gas at least once a day.

The complex microbial fermentation broth of the present invention may preferably have a microbial concentration of about 2 to 8×10 ⁸ cfu/ml.

In one embodiment of the present invention, the complex microbial fermentation broth may further include 1 to 5 parts by weight of eggplant and ginger extracts based on 100 parts by weight. The eggplant and ginger extracts function to improve the deodorizing and antibacterial performance of the complex microbial fermentation broth. The extract may be preferably used when extracted with a mixed solvent of ethanol and butylene glycol, and the mixing ratio may be 4:6 to 2:8 by weight.

In one embodiment of the present invention, the complex microbial fermentation broth further comprises 1 to 5 parts by weight of Eriophorum scheuchzeri ssp. arcticum extract with 1 to 5 parts by weight of the eggplant and ginger extracts based on 100 parts by weight can do.

The arctic stork extract performs the function of greatly improving the deodorizing performance and antibacterial performance of the complex microbial fermentation broth. The extract may be preferably used when extracted with a mixed solvent of ethanol and butylene glycol, and the mixing ratio may be 4:6 to 2:8 by weight.

Arctic storkgrass is a grass that is often found in arctic tundra wetlands, and its seeds are characterized as cotton wool. The Arctic stork extract may be more preferably used as an extract extracted from white cotton, which is a seed, but a component extracted from the stem may also be used.

The complex microbial fermentation broth of the present invention may be used as it is or after additional fermentation. During the additional fermentation, for example, it can be obtained by fermentation in a medium containing molasses. At this time, the molasses and water constituting the medium are preferably contained in a volume ratio of 1:8 to 25, and the complex microorganism and the medium are preferably used in a volume ratio of 1:8 to 25.

The fermentation is preferably carried out at a temperature of 25 to 30 ° C for 2 to 5 days, particularly 3 to 4 days. Molasses is a by-product of the sucrose manufacturing process, and there is no particular limitation on the type of molasses used in the present invention, and either sugar cane molasses or sugar beet molasses can be used, waste molasses, refined molasses, frozen molasses, edible molasses Any of molasses may be used.

The complex microbial fermentation broth according to the present invention contains a large amount of organic acid of 0.25% or more, which is the acidity standard of liquid fermentation deodorant.

The complex microbial fermented liquid of the present invention can be mixed with livestock feed and fed to livestock or sprayed in or around barns. For example, the fermentation broth is diluted in an appropriate ratio, for example, 100 to 200 times in a liquid state, and 300 to 500 ml per 3.3 m 2 is sprayed, or the fermentation broth is freeze-dried and powdered, and mixed with feed at 5 to 10 kg per ton. can pay

Hereinafter, the present invention will be described in more detail through preparation examples and examples. However, the following examples are intended to explain the present invention in more detail, and the scope of the present invention is not limited by the following examples. The following examples may be appropriately modified or changed by those skilled in the art within the scope of the present invention.

Example 1: Production of complex microbial fermented liquid for livestock gas removal and livestock health promotion

After filling 10 liters of water in a container with a capacity of 20 liters, 1 liter of molasses was added and mixed well by shaking. Here, Bacillus subtilis KCTC 3560 culture medium 200ml (1X10 ⁵ ~ 1X10 ⁸ cfu / ml) as bacillus, Bacillus licheniformis KCTC18316P culture medium 200ml (1X10 ⁵ ~ 1X10 ⁸ cfu / ml), Lactobacillus acidophilus KCTC as lactic acid bacteria 3164 culture medium 50ml (1X10 ⁵ ~ 1X10 ⁸ cfu/ml), Lactobacillus brevis KCCM12355P culture medium 50ml (1X10 ⁵ ~ 1X10 ⁸ cfu/ml), Lactobacillus casei KCTC 2180 culture medium 50ml (1X10 ⁵ ~ 1X10 ⁸ cfu/ml), And Lactobacillus reuteri KCCM 10651P culture medium 50ml (1X10 ⁵ ~ 1X10 ⁸ cfu / ml), Rhodopseudomonas capsulata KCTC 2583 culture medium 200ml (1X10 ⁵ ~ 1X10 ⁸ cfu / ml) as a photosynthetic bacterium, and complex vitamins (vitamin A, vitamin D , vitamin E, vitamin B3, and vitamin B7 in a weight ratio of 0.835: 0.003: 0.002: 0.12: 0.04) were mixed with 200 g. The lid was tightly closed and kept in a place where the temperature was maintained between 25 ° C and 30 ° C. Gas was generated during the fermentation process, so the gas was removed at least once a day. After 3-4 days, when the lid was opened, a fermented liquid with a fragrant and sour fermentation odor was obtained. The obtained multimicrobial fermentation broth was confirmed to have a microbial concentration of about 2-8×10 ⁸ cfu/ml.

Example 2: Production of complex microbial fermented liquid for livestock gas removal and livestock health promotion

Based on 100 parts by weight of the complex microbial fermentation broth of Example 1, a complex microbial fermentation broth was prepared in the same manner as in Example 1, except that 3 parts by weight of the eggplant and ginger extracts prepared in Preparation Example 1 were further added.

<Preparation Example 1: Preparation of eggplant and ginger extract>

After 1 kg of dried eggplant and 1 kg of ginger were minced, they were roasted for 20 minutes. Thereafter, the roasted eggplant was put into 4 L of a mixed solvent of ethanol (concentration 40% (v/v)) and butylene glycol (weight ratio 3: 7), and extracted while stirring at 50 to 60 ° C. for 4 hours. The extract was filtered under reduced pressure with a vacuum pump and Whatman No. 2 filter paper, and the filtered extract was concentrated using a vacuum rotary evaporator and spray-dried to obtain a powdered extract.

Example 3: Production of complex microbial fermented liquid for livestock gas removal and livestock health promotion

Based on 100 parts by weight of the complex microbial fermentation broth of Example 1, 3 parts by weight of the eggplant and ginger extracts of Preparation Example 1 and 3 parts by weight of the Arctic stork extract prepared in Preparation Example 2 were further added to Example 1 and A complex microbial fermentation broth was prepared in the same manner.

<Preparation Example 2: Preparation of Arctic sage grass extract>

After cutting 1 kg of dried arctic stork cotton, put it in 2 L of a mixed solvent of ethanol (concentration 40% (v/v)) and butylene glycol (weight ratio 3:7), and at 50 to 60 ° C for 4 hours. Extracted while stirring. The extract was filtered under reduced pressure with a vacuum pump and Whatman No. 2 filter paper, and the filtered extract was concentrated with a vacuum rotary evaporator and then spray-dried to obtain a powdered extract from the root of arctic willow.

Test Example 1: Evaluation of deodorization performance

20 ml of the complex microbial fermentation broth of Examples 1 to 3 were each put into a 5 liter reactor and sealed. The initial concentration of the test gas was set to 100 μmol/mol (ppm) of ammonia, 30 μmol/mol (ppm) of trimethylamine, 50 μmol/mol (ppm) of hydrogen sulfide, and 4 μmol/mol (ppm) of methyl mercaptan. After injection, the concentration of the test gas was measured at the initial (0 min), 30 min, 60 min, 90 min, and 120 min. The concentration of the test gas was measured according to KS I 2218: 2009. During the test, the temperature was maintained at 23°C ± 5°C and the humidity was maintained at 50% ± 10%. Separately, the same test as the above process was performed in the absence of a sample, and this was referred to as the blank concentration (control group). The removal rate of the test gas for each time period was calculated by the following formula.

Test gas removal rate (%) = [{(blank concentration)-(sample concentration)/(blank concentration)} X 100

The experimental results are shown in Table 1 below.

Elapsed time (minutes) control group
Gas concentration (ppm) Example 1 sample-gas concentration (ppm) Example 2 Sample-gas concentration (ppm) Example 3 Sample-gas concentration (ppm) Ammonia (NH ₃ ) 0 100 100 100 100 30 100 2 1.8 1.0 60 99 0 0 0 90 99 0 0 0 120 99 0 0 0 Triethylamine (CH ₃ ) ₃ N) 0 30 30 30 30 30 30 One 0.8 0.5 60 29 0 0 0 90 29 0 0 0 120 28 0 0 0 Hydrogen sulfide (H ₂ S) 0 50 50 50 50 30 49 22 20 16 60 49 22 20 16 90 48 21 18 14 120 48 21 18 14 Methylmercaptan (CH ₃ SN) 0 4.0 4 4 4 30 4.0 2.9 2.8 2.4 60 4.0 2.9 2.8 2.4 90 4.0 2.8 2.6 2.2 120 4.0 2.8 2.6 2.2

As shown in Table 1, it was confirmed that the fermentation broth of the present invention exhibits very good deodorizing performance for various gases.

Test Example 2: Evaluation of antibacterial performance

Antibacterial activity test for Salmonella typhimurium IFO 14193, Klebsiella pneumoniae ATCC 4352 and MRSA bacteria (Staphylococcus aureus subsp. aureus) ATCC 33591 of the complex microbial fermentation broth of Examples 1 to 3 KCL-FIR-1002: 2011 (antibacterial processed products, antibacterial test method, antibacterial effect).

Specifically, after smearing each test strain on each sample, the initial state and the state after 24 hours were visually evaluated, and the test environment was 30.2 ± 0.2% R.H. at 37.1 ± 0.1 ° C. The results are shown in Table 2 below. For comparison, a blank test group was used as a control group.

initial concentration Concentration after 24 hours (cfu/ml) control group
(cfu/ml) Example 1 Sample - (cfu/ml) Example 2 Sample - (cfu/ml) Example 3 Sample - (cfu/ml) Salmonella 1.5 × 10 ⁴ 5.6 × 10 ⁴ < 10 < 8 < 2 pneumococci 1.4 × 10 ⁴ 4.5 × 10 ⁴ < 10 < 7 < 4 MRSA bacteria 1.3 × 10 ⁴ 4.5 × 10 ⁴ < 10 < 8 < 4 Escherichia coli 1.6 × 10 ⁴ 6.1 ×10 ⁴ < 10 < 8 < 2 Pseudomonas aeruginosa 1.7 × 10 ⁴ 6.5 × 10 ⁴ < 10 < 7 < 4 Staphylococcus aureus 1.3 × 10 ⁴ 4.1 ×10 ⁴ < 10 < 8 < 4

As shown in Table 1, it was confirmed that the complex microbial fermentation broth of the present invention exhibits very good sterilization performance against various harmful bacteria.

Test Example 3: Measurement of antibacterial activity of plant extracts

The antibacterial activity of eggplant and ginger extracts (Preparation Example 1) and Arctic japonica extract (Preparation Example 2) and their mixed extracts (1:1 weight ratio) was evaluated in the following manner.

Representative food poisoning bacteria (Bacillus cereus, Bacillus subtilis, Campylobacter jejuni, Escherichia coli, Escherichia coli O-157, Listeria monocytogenes, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella enteritidis, Salmonella thypimurium, Vibrio parahaemolyticus, Yersinia enterocolitica) were used.

The bacteria were inoculated with 50 μl of the culture in 10 ml of sterilized TSB medium and subcultured at 30° C. for 24±1 hours. The concentration of this culture maintained Abs 0.6±0.1 at a microplate reader absorption wavelength of 655 nm. The subcultured test bacteria were diluted 10 ^-3 times with sterile distilled water, and the disc diffusion method was performed. That is, the diluted strain is spread on a 4 mm thick plate medium by applying it to a sterile cotton swab, and a sterilized 8 mm (thickness 1 mm) filter is placed on the eggplant and ginger extracts (Preparation Example 1) and Arctic stork extract (Preparation Example 2) and these 50 μl of each of the mixed extracts (1:1 weight ratio) was injected. After inhalation for about 20 minutes, the cells were cultured at 30°C for 24±1 hours, and growth circles were confirmed.

The antibacterial activity was evaluated by measuring the size of the transparent zone including filter paper, and the results are shown in Table 3 below.

strain name strain number ^a Antibacterial activity (mm) ^b Eggplant and ginger extract (Preparation Example 1) Arctic stork extract (Preparation Example 2) Eggplant & Ginger Extract+ Arctic Stork Extract bacteria Bacillus cereus KCTC 1012 13 15 16 Bacillus subtilis KCCM 1021 15 16 17 Campylobacter jejuni KCCM 41772 14 15 16 Escherichia coli ATCC 25922 15 16 17 Escherichia coli O-157 ATCC 43888 14 15 16 Listeria monocytogenes ATCC 15313 14 15 16 Pseudomonas aeruginosa KCTC 1750 14 16 17 Staphylococcus aureus ATCC 25923 14 15 16 Salmonella enteritidis KCCM 11862 14 15 16 Salmonella thypimurium ATCC 13076 15 16 17 Vibrio parahaemolyticus ATCC 17802 13 16 17 Yersinia enterocolitica ATCC 23715 15 16 17

Inc. ^a : ATCC American Type Culture Collection, KCTC Korea Research Institute of Bioscience and Biotechnology Biological Resources Center; KCCM Korea Incubation Association

^b : size of transparent zone (diameter mm)

As confirmed in Table 3, eggplant and ginger extracts (Preparation Example 1), arctic stork extract (Preparation Example 2), and their mixed extracts (1: 1 weight ratio) are all excellent antibacterial agents against all bacteria. showed activity.

Test Example 4: Confirmation of Safety of Arctic Stork Grass Extract

Experimental animals were 4-week-old male Sprague-Dawley rats (Central Lab animals, Seoul, Korea) fed pellet-type solid feed (jeil animal feed) in an environment-controlled breeding room (temperature 20°C, humidity 55%, 12-hour dark/light cycle). Co., Daejeon, Korea), and a toxicity test of the Arctic sage grass extract (Preparation Example 2) was conducted using 6-week-old mice whose average body weight had grown to about 200 g by breeding for 2 weeks.

Specifically, 5 each of the 4-week-old rats were fed 30 ml of the Arctic sage grass extract (Preparation 2) per day together with a pellet-type solid feed (Jeil animal feed Co., Daejeon, Korea) for 4 weeks to reduce toxicity. evaluated.

As a result of the toxicity evaluation, it was confirmed that none of the rats participating in the experiment died, and all 10 rats maintained their health for 4 weeks.

Claims (7)

Bacillus subtilis ( Bacillus subtilis ), Bacillus Licheniformis ( Bacillus Licheniformis ), lactic acid bacteria ( Lactobacillus ), photosynthetic bacteria, and complex microbial fermented broth for removing livestock gas and promoting livestock health, including
The lactic acid bacteria include Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus casei, and Lactobacillus reuteri,
The photosynthetic bacteria is Rhodopseudomonas capsulata,
The multivitamin contains vitamin A, vitamin D, vitamin E, vitamin B3, and vitamin B7 in a weight ratio of 0.8 to 0.9: 0.001 to 0.005: 0.001 to 0.003: 0.08 to 0.15: 0.01 to 0.06,
The Bacillus subtilis ( Bacillus subtilis ), Bacillus licheniformis ( Bacillus Licheniformis ), lactic acid bacteria ( Lactobacillus ), photosynthetic bacteria, and complex vitamins are each 1-1.5: 1-1.5: 1-1.5: 1-1.5: 1- It has a weight ratio of 1.5, and the Bacillus subtilis, Bacillus Licheniformis, Lactobacillus, and photosynthetic bacteria are a culture medium having a viable cell count of 1X10 ⁵ to 1X10 ⁸ cfu / ml,
Based on 100 parts by weight of the complex microbial fermentation broth, extracts 1 to 5 obtained by extracting a mixture of eggplant and ginger in a 1: 1 weight ratio with a mixed solvent in which ethanol and butylene glycol were mixed in a weight ratio of 4: 6 to 2: 8 Characterized in that it further comprises 1 to 5% by weight of an extract obtained by extracting Eriophorum scheuchzeri ssp. arcticum cotton with a mixed solvent of ethanol and butylene glycol at a weight ratio of 4:6 to 2:8 Complex microbial fermented liquid for removing livestock gas and improving livestock health. delete delete delete delete delete According to claim 1,
The complex microbial fermented solution is a complex microbial fermentation solution for livestock gas removal and livestock health promotion, characterized in that it is used for feeding livestock by mixing with livestock feed or for spraying in or around the barn.