KR102523776B1 - Composition and health functional food containing Bifidobacterium lactis IDCC 4301 having vitamin A production ability - Google Patents

Abstract

The present invention relates to a composition and health functional food containing Bifidobacterium lactis IDCC 4301 with excellent vitamin A production ability. The Bifidobacterium lactis IDCC 4301 may have been isolated from the stool of a breast-fed infant.

Description

Composition and health functional food containing Bifidobacterium lactis IDCC 4301 having vitamin A production ability}

The present invention relates to a composition and health functional food containing Bifidobacterium lactis IDCC 4301 having excellent vitamin A production ability.

Vitamins are trace amounts of organic substances essential for metabolism in the body and are nutrients for the body's development, daily activities, and maintenance of health. Vitamins are broadly classified into fat-soluble vitamins and water-soluble vitamins. Fat-soluble vitamins accumulate in the body's fat tissue or liver and can be stored for a long time, whereas water-soluble vitamins are not stored in the body and are excreted through urine, so a steady intake is necessary.

Vitamin A (retinol, retinol) is mainly contained in animal foods, and can also be consumed through plant foods in the form of beta-carotene, a precursor. Vitamin A helps maintain eye health, as well as skin, hair and teeth health. In addition, vitamin A is known to be involved in growth and development through the improvement of immune function through the promotion of antibodies, the regulation of the nervous system, and the regulation of growth hormone secretion.

On the other hand, vitamin A deficiency can cause night blindness due to reduced visual function, weakened immune function due to vulnerability to bacterial infection, delayed growth, and dry skin. Accordingly, it is necessary to steadily supplement vitamin A through food or nutritional supplements.

Korean Patent Publication No. 10-2009-0094235 (published on September 04, 2009)

An object of the present invention is to provide a composition and health functional food containing Bifidobacterium lactis IDCC 4301 having excellent vitamin A production ability.

The object of the present invention is achieved by including Bifidobacterium lactis IDCC 4301 [accession number: ATCC BAA-2848] in a composition having a vitamin A producing function.

The Bifidobacterium lactis IDCC 4301 may be isolated from feces of breastfed infants.

The object of the present invention can be achieved by a food having a vitamin A producing function containing 0.1 to 50% by weight of the composition.

The object of the present invention can be achieved by a health functional food having a function of producing vitamin A containing 0.1 to 50% by weight of the composition.

According to the present invention, a composition and health functional food containing Bifidobacterium lactis IDCC 4301 having excellent vitamin A producing ability are provided.

1 is a graph showing a calibration curve of vitamin A, which is a standard substance;
Figure 2 shows the vitamin A production according to the type of lactic acid bacteria.

The present invention will be described in more detail with reference to the following drawings.

Since the accompanying drawings are only examples shown to explain the technical spirit of the present invention in more detail, the spirit of the present invention is not limited to the accompanying drawings. In addition, in the accompanying drawings, sizes and intervals may be exaggerated unlike actual ones in order to explain the relationship between each component.

The present invention relates to a composition containing lactic acid bacteria and capable of producing vitamin A.

The lactic acid bacteria of the present invention include Bifidobacterium lactis IDCC 4301 [accession number: ATCC BAA-2848].

Bifidobacterium lactis IDCC 4301 in the composition may be 1% to 100%, 10% to 90% or 1% to 10% by weight.

The present invention relates to a food composition containing probiotics capable of producing vitamin A, including Bifidobacterium lactis IDCC 4301 [Accession No.: ATCC BAA-2848].

Bifidobacterium lactis IDCC 4301 was isolated from feces of breast-fed infants, and the food composition or health functional food composition contains 0.1 billion to 100 billion or 100 to 100 billion CFU/ may contain g.

The present invention relates to a health functional food containing probiotics capable of producing vitamin A, and contains Bifidobacterium lactis IDCC 4301 [accession number: ATCC BAA-2848] as an active ingredient.

The composition of the present invention may contain lactic acid bacteria other than Bifidobacterium lactis IDCC 4301.

The food composition of the present invention includes compositions for all types of food, such as functional food, nutritional supplements, health food, and food additives. Food compositions of this type can be prepared in various forms according to conventional methods known in the art. For example, as a health food, it can be prepared in the form of gum, vitamin complex, tea, juice and drink containing lactic acid bacteria, and a food composition containing Bifidobacterium lactis IDCC 4301 as an active ingredient can be granulated, encapsulated or It can be powdered and ingested.

The functional food composition of the present invention may include components commonly added during food preparation, and may include, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when prepared as a drink, citric acid, high fructose corn syrup, sugar, glucose, acetic acid, malic acid, fruit juice, jujube extract, licorice extract, and the like may be further included.

Food compositions include various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, It may contain a protective colloidal thickener, a pH adjusting agent, a stabilizer, a preservative, glycerin, alcohol, a carbonating agent used in carbonated beverages, and the like. In addition, it may contain extracts for the production of natural fruit juice, fruit juice beverages and vegetable beverages. These components may be used independently or in combination.

As another example, the formulation of the food composition may be in the form of powders, granules, pills, tablets, capsules, as well as general food or beverage forms.

The type of food is not particularly limited, and examples of food include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, There are drinks, alcoholic beverages, vitamin complexes, and the like, and may include all foods in a conventional sense.

In general, when preparing food or beverage, the amount of Bifidobacterium lactis IDCC 4301 used may be 0.1 to 50% by weight, 0.1 to 20% by weight, or 0.1 to 10% by weight. However, in the case of long-term intake for the purpose of health and hygiene or health control, the amount may be less than the above range.

Among the food compositions according to one embodiment, the beverage may contain various flavoring agents or natural carbohydrates as additional ingredients, like conventional beverages. The aforementioned natural carbohydrates may be monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as thaumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used.

In addition to the above, the food composition according to one embodiment includes various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin , alcohol, carbonation agents used in carbonated beverages. In addition, the food composition of the present invention may contain fruit flesh for preparing natural fruit juice, fruit juice beverages and vegetable beverages. These components may be used independently or in combination.

The present invention will be described in detail through experimental examples below.

[Example 1] Cultivation of probiotics in long-haired medium simulating intake of probiotics

For probiotics culture, a long hair medium containing the ingredients shown in Table 1 was prepared and then sterilized at 121° C. for 15 minutes. Then, the single colony of probiotics cultured on the plate was cultured anaerobically at 37 ° C for 1 day in a long wool broth, and Bacillus subtilis and Bacillus coagulans were cultured at 43 ° C and 45 ° C, respectively, under aerobic conditions. did The culture medium was centrifuged at 4°C and 6,000 rpm to recover the supernatant, and concentrated 10 times using a concentrator. Finally, the supernatant was filtered using a filter having a size of 0.45 μm, and a sample for analysis was prepared.

ingredient Content (Standard 1L) tryptone peptone 2g yeast extract 1g glucose 400 mg L-cysteine 500 mg cellobiose 1g maltose 1g fructose 1g meat extract 5g potassium phosphate 100 mL Magnesium Sulphate Heptahydrate 2mg sodium bicarbonate 400 mg sodium chloride 80 mg calcium chloride 1mL vitamin K 1mL iron sulfate 1 mL Histidine/hematin solution 1 mL Polysorbate 80 2mL ATCC Vitamin Blend 10 mL ATCC trace element mixture 10 mL acetic acid 1.7 mL isovaleric acid 0.1 mL propionic acid 2mL butyric acid 2mL resazurin 4mL

[Example 2] Quantitative analysis of vitamin A

The prepared samples were analyzed using liquid chromatography (Nanospace SI-2 3023, SHISEIDO, Tokyo, Japan). A CAPCELL PAK UG 120 S5 column (4.6 mm id x 250 mm) was used for separation and quantification of substances, and tertiary distilled water was used as mobile phase A, and a mixture of 90% acetonitrile + 10% was used as mobile phase B. The mobile phase was flowed at a flow rate of 0.8 ml/min. The column temperature was maintained at 37° C., and 20 μl of the sample was injected to confirm the production of vitamin A1 using a UV detector with a wavelength of 280 nm.

The standard concentration of vitamin A1 was prepared at 1 mg/mL, and as shown in FIG. 1, it was confirmed that the reliability of the calibration curve was 99.9% (R2=0.99948) or higher. Based on this, vitamin A1 production in the probiotics supernatant was quantified and shown in FIG. 2, and vitamin A1 production ability was compared for each strain.

As a result of culturing a total of 6 types of probiotics in the long-haired medium, it was confirmed that Bifidobacterium lactis IDCC 4301 had a remarkably excellent vitamin A1 production ability.

Claims (4)

In the composition having a vitamin A producing function,
A composition comprising Bifidobacterium lactis IDCC 4301 [accession number: ATCC BAA-2848]. According to claim 1,
The Bifidobacterium lactis IDCC 4301 is a composition isolated from feces of breast-fed infants. A food having a function of producing vitamin A, comprising 0.1 to 50% by weight of the composition according to any one of claims 1 and 2. A health functional food having a function of producing vitamin A, comprising 0.1 to 50% by weight of the composition according to any one of claims 1 and 2.

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