KR102511701B1 - Functional collagen composition using collagen amino acid derived from Aurea Helianthus - Google Patents

Abstract

The present invention is an extract or fraction from the leaves, stems, and roots of Aurea Helianthus , or glycine ( Glycin ), proline ( Proline ), and hydroxyproline ( hydroxyproline ), which are separated by ultrasonic treatment. Effectiveness of any one collagen amino acid Provided is a functional collagen composition using collagen amino acids derived from goldfish, characterized in that it contains components. At this time, the gold flower ( Aurea Helianthus ) is washed by selecting any one of the leaves, stems, and roots dried in the shade after cultivation to remove foreign substances, dried in the shade at 35 ~ 45 ℃, and then pulverized using a grinder, Using 6 L of 20% alcohol per 1 kg of pulverized material, extract for 2 to 5 days in a shaking incubator at 150 RPM and 15 ° C. Plant residues were removed by filtration, and concentrated under reduced pressure with an evaporator at 30 to 40° C. to remove 20% alcohol, and then distilled water was added to obtain a final 4L, 2.9 to 3.0% Brix extract. The extract was in an ultrasonic extractor. Ultrasound frequency 20kHz, amplitude 60%, ultrasound treatment for 3 to 4 hours under the conditions of 4 ℃, glycine ( Glycin ), proline ( Proline ), hydroxyproline ( hydroxyproline ), any one collagen amino acid extract
Accordingly, the present invention completely acquires collagen amino acids obtained from Keumhwagyu in the most efficient way, thereby solving the weak point of animal collagen, while improving joint health, skin aging and wrinkles. It can be used as a variety of raw materials for cosmetic raw materials, functional food raw materials, etc., as well as free formulation changes can be easily pursued, and in particular, collagen amino acid (GPH; glycine, proline), a component similar to skin cells that helps collagen synthesis in functional and organic aspects . there is

Description

Functional collagen composition using collagen amino acid derived from Aurea Helianthus}

The present invention relates to a functional collagen composition using the collagen amino acid contained in Keumhwagyu, and more specifically, to obtain intact collagen amino acid obtained from Geumhwagyu in the most efficient way, thereby resolving the weakness of animal collagen and maintaining joint health. ㆍGeumhwagyu-derived collagen that can be used as a variety of raw materials for pharmaceutical raw materials effective in improving skin aging and wrinkles, as well as quasi-drug raw materials, cosmetic raw materials, and functional food raw materials, as well as functional and organically free formulation changes It relates to a functional collagen composition using amino acids.

In general, collagen (Collagen) is also called light protein or collagen (膠原質), widely distributed in multicellular animals such as invertebrates and vertebrates, and is the light protein that is found most in quantity. In mammals, it occupies about 1/4 of the total protein, and the fiber-like tension constituting animal connective tissue is very strong, and it transmits power without loss in tendons or ligaments. The basic structural unit of collagen is tropocollagen with a molecular weight of about 300,000, and this molecule has a three-stranded polypeptide chain in a right-twisted triple helix structure. The tropocollagen molecules associate to form collagen fibers (collagen fibers), and each molecule is arranged 1/4 offset in the axial direction to form a unique stripe at 64 nm intervals. and become insoluble.

However, when collagen is boiled for a long time in hot water, dilute acid, or dilute alkali, it turns into gelatin, a induced protein that is soluble in water. Collagen peptides are a type of fibrous protein composed of glycine, proline, hydroxyproline, glutamic acid, etc., with few amino acids containing sulfur among amino acids. , It mainly exists in membranes surrounding organs in the human body, articular cartilage, eye cornea, bones, and skin.

On the other hand, the main function of collagen is known to be the firmness of the skin, the resistance and binding force of tissues, and the support of cell adhesion. The thickness of the skin becomes thinner due to aging and photoaging by UV irradiation, and this phenomenon is known to be closely related to the formation of wrinkles in the skin.

Recently, as extensive research has progressed, various functions of collagen have been revealed. These collagen peptides are increasingly used as health foods, surgical and ophthalmic treatments, or cosmetic additives. As for these collagen peptides, many studies have been conducted so far on product stability and skin and body absorption rates from the food, pharmaceutical, and cosmetic viewpoints, and accordingly, utilization plans are being actively conducted.

Prior art related to this is Korean Patent Publication No. 10-1999-0033721 (Prior Document 1), Korean Patent Registration No. 10-0381741 (Prior Document 2), Korean Patent Publication No. 10-2004-0014618 ( Prior Document 3), Republic of Korea Patent Registration No. 10-0827389 (Prior Document 4), and the like.

Prior Document 1 mentions a method for extracting and separating collagen from fish skins, heads, bones, intestines, etc. discarded from fish (cat fish), and a method of producing water-soluble collagen with citric acid or ascorbic acid from this and a method for producing functional cosmetics (pack, essence) based on the water-soluble collagen obtained in this way is proposed. In addition, we propose a method for producing a functional collagen fermented beverage by fermenting organic acid with fruit juice using the backbone and head of cat fish having a high collagen content.

Prior Document 2 at least partially contains a seafood-derived collagen compound obtained from a marine animal subjected to deodorization treatment as an active material capable of simultaneously performing a cross-linking treatment capable of improving the mechanical properties of collagen during any manufacturing step of the collagen compound. We present a collagen product.

Prior Document 3 is prepared by dividing cucumber (50%), Angelica (10%), Usul (6%), Eucommia (5%), arrowroot (4%), jujube (4%), and licorice (1%) into equal parts. We present a method for manufacturing a collagen ogapi extract beverage in which the effect of each material is doubled by mixing and maturing the processed extracted vegetable extract liquid, collagen meat (95%), and animal extract liquid extracted from manufacturing and processing ginger (5%).

Prior Document 4 is a water-soluble collagen solution obtained by passing each of the water-soluble collagen solutions obtained by pretreatment and hot water treatment of vegetable, animal, and marine raw materials through an activated carbon layer, enzymatically reacting the water-soluble collagen solution in a weak acid solution and passing through the activated carbon layer to obtain each hydrolyzed collagen. get the peptide. A collagen peptide composition for food and a manufacturing method for obtaining a food or cosmetic composition by mixing these collagen peptide solutions individually or in a constant ratio are proposed.

However, according to the preceding documents, all of them are proposed as foods and cosmetics using collagen, etc., but there is a problem in that the efficacy of collagen cannot be sufficiently exerted because the reaction between collagen and other additives is hardly considered. , Moreover, even if the efficacy of collagen is exerted, it simply depends on collagen, so there is a disadvantage in that the value of a high value-added material is reduced.

Moreover, in the recent hard capsule industry, demand is shifting from animal to vegetable capsules, and the production volume of vegetable capsules is rapidly increasing. Demand for plant collagen is increasing. As such, vegetarian capsules are natural, GMO, vegetable shells, free from animal derivatives, gluten and modified sugars, and can be divided into several types such as plant polysaccharides (Pululan), starch and HPMC (Hydroxyl Propyl Methyl Celluloses). These vegetarian capsules have the advantages of being suitable for halal or kosher certification, pure, clean and elegant appearance with no taste or odor, ideal for improving the bioavailability of useful ingredients, easy to swallow, safe, non-carcinogenic and difficult to change in structure.

Accordingly, with the development of vegetable protein (hereinafter referred to as “vegetable collagen”) containing the main components of collagen, such as hydroxyproline and hydroxylysine , we select raw materials from animal materials used with anxiety. As the need to compensate for the weakness of manufactured animal collagen is on the rise, the present applicant intactly acquires collagen amino acids obtained from plants, that is, Geumhwagyu, and solves the weakness of animal collagen while helping to synthesize collagen similar to skin cells. The development of the present invention was completed after confirming that the content of collagen amino acid (GPH; glycine, proline, hydroxyproline), which is a component, is very high, so that it can be used as various raw materials for pharmaceutical raw materials, quasi-drug raw materials, cosmetic raw materials, functional food raw materials, etc. did

Republic of Korea Patent Publication No. 10-1999-0033721 "Method for extracting water-soluble COLLAGEN from fish and manufacturing method for functional COLLAGEN cosmetics and COLLAGEN fermented beverage" (published on May 15, 1999) Republic of Korea Patent Registration No. 10-0381741 "Collagen product containing seafood-derived collagen having low odor and improved mechanical properties, its use as a cosmetic or pharmaceutical composition or product" (Registration date: 2003. 04. 12. ) Republic of Korea Patent Publication No. 10-2004-0014618 "Method for manufacturing collagen extract extract drink" (Publication date: 2004. 02. 14.) Republic of Korea Patent Registration No. 10-0827389 "Collagen peptide composition for food and manufacturing method thereof" (Registration date: 2008. 04. 28.)

The purpose of the present invention to fundamentally improve the above conventional problems is to completely acquire collagen amino acids obtained from Geumhwagyu in the most efficient way, thereby solving the weak points of animal collagen while improving joint health, skin aging and wrinkles. In addition to being used as various raw materials for pharmaceutical raw materials that are effective in improvement, raw materials for quasi-drugs, raw materials for cosmetics, raw materials for functional foods, etc., skin cells that can easily pursue changes in free formulations and help synthesize collagen, especially in functional and organic aspects It has a very high content of collagen amino acid (GPH; glycine, proline, hydroxyproline), which is a similar ingredient, so it is absorbed quickly in the body and is involved in the amount of moisture in the skin. It is intended to provide a functional collagen composition using collagen amino acids derived from gold fleas with excellent product preservation and safety, such as moisturizing and wrinkle improvement.

The present invention for achieving the above object, after cultivating Aurea Helianthus, selects any one of the shade-dried leaves, stems, and roots to wash them to remove foreign substances, dry them in the shade at 35 to 45 ° C, and then grind them , and extracted for 2 to 5 days in a shaking incubator at 150 RPM and 15 ° C using 6L of 20% alcohol per 1 kg of the pulverized product, and the collagen content of the extract is high, so that the thickness is 1 mm × 1 mm After filtering 4-5 times through a filter net to remove plant residues, concentrate under reduced pressure with an evaporator at 30-40 ° C to remove 20% alcohol, and then add distilled water to obtain a final 4L, 2.9-3.0% Brix extract. , The extract is glycine, proline, and hydroxyproline ( For joint health, skin aging, and wrinkle improvement containing active ingredients of collagen amino acid of any one of glycine, proline, and hydroxyproline after extracting any one of collagen amino acid from hydroxyproline. It is characterized in that it is used as any one of cosmetic, food, and pharmaceutical compositions.

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In addition, according to the present invention, the collagen composition is a cosmetic composition, and contains 0.1 to 10% by weight of either a collagen amino acid extract or a freeze-dried powder based on the total weight of the cosmetic composition, and the remaining amount is cosmetic It is characterized by containing in an amount of 90 to 99.9% by weight of the carrier acceptable for the formulation.

In addition, according to the present invention, the collagen composition is a food composition, and contains 0.1 to 10% by weight of either a collagen amino acid extract or a lyophilized powder based on the total weight of the food composition, and the remaining amount is food It is characterized by containing in an amount of 90 to 99.9% by weight of the carrier acceptable for the formulation.

In addition, according to the present invention, the collagen composition is a pharmaceutical composition for joint health, skin aging and wrinkle improvement, and contains 0.1 to 10% by weight of either a collagen amino acid extract or a freeze-dried powder based on the total weight of the pharmaceutical composition And, it is characterized in that it is contained in an amount of 90 to 99.9% by weight of the carrier acceptable for pharmaceutical formulations in the remaining amount.

On the other hand, prior to this, the terms or words used in this specification and claims should not be construed as being limited to ordinary or dictionary meanings, and the inventors use the concept of terms to explain their invention in the best way. Based on the principle that it can be properly defined, it should be interpreted as meaning and concept consistent with the technical spirit of the present invention. Therefore, the embodiments described in this specification and the configurations shown in the drawings are only one of the most preferred embodiments of the present invention, and do not represent all of the technical ideas of the present invention, so various alternatives can be made at the time of this application. It should be understood that there may be equivalents and variations.

As described in the above configuration and action, the functional collagen composition using the collagen amino acid derived from Keumhwagyu according to the present invention completely acquires the collagen amino acid obtained from Geumhwagyu in the most efficient way, and solves the weakness of animal collagen while joint joint It can be used as a variety of raw materials for pharmaceutical raw materials that are effective for health, skin aging and wrinkle improvement, as well as quasi-drug raw materials, cosmetic raw materials, and functional food raw materials. Collagen amino acid (GPH; glycine, proline, hydroxyproline), a component similar to skin cells that help synthesize collagen, is very high in content, so it is quickly absorbed into the body and is involved in the amount of moisture in the skin, preventing skin aging, weakening of joints and blood vessel damage when ingested In addition, it provides excellent effects in product preservation and safety, such as moisturizing the skin and improving wrinkles when applied.

Figure 1 is a test report showing the amino acid analysis through the hot water extract of Geumhwagyu according to the present invention.
Figure 2 is a test report showing the content of hydroxyproline ( hydroxyproline ) through the geumhwagyu hot water extract according to the present invention.

Hereinafter, preferred embodiments according to the present invention will be described in detail with reference to the accompanying drawings.

Unless otherwise defined, all technical terms used in the present invention have the following definitions, and are used in the meaning commonly understood by those skilled in the art in the field related to the present invention. In addition, although preferred methods or samples are described in this specification, similar or equivalent ones are also included in the scope of the present invention. In addition, the contents of all publications described by reference in this specification are incorporated into the present invention. The term approximately means 30, 25, 20, 25, 10, 9, 8, 7, 6, 5, 4 for a reference amount, level, value, number, frequency, percentage, measure, size, amount, weight, or length. It means an amount, level, value, number, frequency, percentage, size, size, weight or length that varies by 3, 2 or 1%.

The present invention proposes a functional collagen composition using collagen amino acids extracted from the leaves, stems and roots of Aurea Helianthus . In particular, collagen amino acids (GPH: Glycine-Proline-Hydroxyproline) obtained from Keumhwagyu are acquired intact in the most efficient way, and pharmaceutical raw materials that are effective in improving joint health, skin aging, and wrinkles while addressing the weaknesses of animal collagen. It relates to functional collagen compositions that can be used as various raw materials for quasi-drug raw materials, cosmetic raw materials, and functional food raw materials.

According to the present invention, extracts, fractions, or extracts from the leaves, stems, and roots of Aurea Helianthus , or glycine ( Glycin ), proline ( Proline ), and hydroxyproline ( hydroxyproline ) separated from the same by ultrasonic treatment, any one collagen amino acid Characterized in that it contains an active ingredient.

On the other hand, Geumhwagyu (金花葵), whose scientific name is " Aurea Helianthus ", is an annual herbaceous plant and is also called wild bouillon. It was known that it became extinct in the 1980s because of its medicinal value, but it is said that a professor at the Chinese Academy of Agricultural Sciences accidentally kept it and discovered it. According to Herbal Medicine, Geumhwagyu (金花葵), a rare medicinal plant that is so rare that it is called "the true bear of the vegetable world," has antipyretic, detoxifying, anti-inflammatory, analgesic effects, oral pain, swelling, topical treatment of burns, spleen and It is said to be used in the treatment of various malignant pains such as gastric and muscle tissue effects, therapeutic indigestion, anorexia, and bruises.

In addition, it is said to help reduce or prolong middle-aged menopausal syndrome as it is rich in natural phytoestrogens. According to modern medical research, analgesic, anti-cerebral ischemia and treatment of oral ulcers, regulate endocrine, immune system, increase body resistance, improve cardiovascular function and microcirculation, anti-oxidation of the body, anti-cardiac ischemia, hypoxia, inflammation, pain village, anti-fatigue, aging It can improve anti-cancer, cholesterol-lowering effects. Geumhwagyu flowers are picked from the end of July, torn into small pieces, and dried. Because they are rich in collagen, they are a food. It is used as a raw material for cosmetics and tea. Geumhwagyu is rich in collagen not only in flowers and leaves, but also in flower stalks and roots. .

Extracts, fractions, or extracts from the leaves, stems, and roots of Aurea Helianthus , or collagen amino acid active ingredients of any one of glycine, proline , and hydroxyproline separated by sonication therefrom contain Therefore, in the present invention, "extract" is a concept including all extracts, fractions, and purified products obtained in each step of extraction, fractionation, or purification, and dilutions, concentrates, or dried products thereof.

At this time, according to the detailed configuration according to the present invention, the Aurea Helianthus is washed by selecting any one of the leaves, stems, and roots dried in the shade after cultivation to remove foreign substances, and then dried in the shade at 35 to 45 ° C. Pulverized using a grinder, and extracted for 2 to 5 days in a shaking incubator at 150 RPM and 15 ° C using 6 L of 20% alcohol per 1 kg of the ground material, and the collagen content of the extract is high, so that 1 mm × 1 mm Plant residues were removed by filtering 4-5 times through a thick filter net, then concentrated under reduced pressure with an evaporator at 30-40°C to remove 20% alcohol, and then distilled water was added to obtain a final 4L, 2.9-3.0% Brix extract. It is characterized by doing. After cultivating Geumhwagyu, select one of the shade-dried leaves, stems, and roots, wash it with purified water to remove impurities (soil and foreign matter), dry it in the shade at 40 ° C, and use a grinder to It is finely pulverized to a size of 10 to 100 μm or less per 1 kg. Then, using 6 L of 20% alcohol per 1 kg of Geumhwagyu roots, extract for 2 to 5 days, preferably 3 days, under conditions of 150 RPM and 15 ° C in a shaking incubator. Subsequently, the extract has a high collagen content and is filtered 4 to 5 times through a 1mm × 1mm thick filter net to remove plant residues, and then concentrated under reduced pressure in an evaporator at 30 to 40° C., preferably at 37° C., to obtain 20% After removing the alcohol, distilled water is added to obtain a final 4L extract. The Brix of these extracts was measured at 2.9-3.0%.

On the other hand, the above extract may be further subjected to a conventional fractionation process, and may be purified using a conventional purification method. For example, the Geumhwagyu extract included in the functional collagen composition of the present invention can be prepared in a powder state by an additional process such as freeze-drying or spray-drying the extract.

In addition, the filter net of the present invention is a process of removing floating solid particles from the extract, and can filter out particles using one selected from cotton, nylon, and paper, or use ultrafiltration, cryofiltration, centrifugation, etc. , but not limited thereto. In addition, a separation process by chromatography (chromatography according to size, charge, hydrophobicity or affinity) may be further included.

On the other hand, the extract of the present invention may be freeze-dried, vacuum-dried, hot-air-dried, spray-dried, vacuum-dried, foam-dried, radiofrequency-dried, and infrared-dried, for example, but is not limited thereto. In some cases, a step of pulverizing the finally dried extract may be further included.

In addition, according to the detailed configuration according to the present invention, the extract is ultrasonically treated for 3 to 4 hours under the conditions of an ultrasonic frequency of 20 kHz, an amplitude of 60%, and 4 ° C in an ultrasonic extractor to obtain glycine and proline ( It is characterized by extracting any one of collagen amino acids from Proline ) and hydroxyproline . In the extract obtained through the above-mentioned Geumhwagyu extraction method, any one of collagen amino acids among glycine , proline , and hydroxyproline is extracted through sonication as shown in Table 1 below. This extract is obtained by ultrasonication of the obtained extract in an ultrasonic extractor at an ultrasonic frequency of 20 kHz, an amplitude of 60%, and ultrasonic treatment for 3 to 4 hours at 4 ° C to obtain glycine, proline , and hydroxyproline . ) Extract any one of the collagen amino acids.

As such, looking at the amino acid analysis results for the extract through the KFRI Korea Food Research Institute as shown in Figure 1, glycine ( Glycin ) is 517.7㎎ / 100g, proline ( Proline ) is 246.3㎎ / 100g, lysine ( lysine ) is 277㎎ / 100g As a result of analyzing the content of hydroxyproline in the extract through the Korea Functional Food Research Institute, as shown in FIG. 2, hydroxyproline is abundant at 0.28 mg/g. In addition, it was confirmed that various amino acids such as glutamic acid , aspartic acid , and arginine , which are useful in moisturizing and preventing skin aging, are abundantly contained.

At this time, according to one embodiment of the present invention, the collagen composition contains 0.1 to 10% by weight of either an extract or a lyophilized powder based on the total weight of the cosmetic composition, and the remaining amount is a cosmetic preparation Characterized in that it contains in an amount of 90 to 99.9% by weight of the carrier allowed for. In this way, the functional collagen composition extracted from the Keumhwagyu is identified as vegetable collagen as it contains abundant glycine , proline , and hydroxyproline , and such a functional collagen composition can be used for manufacturing a cosmetic composition, When looking at the cosmetic composition based on the total weight of the functional collagen composition, it is desirable to select either extract or freeze-dried powder and maintain it within the range of 0.1 to 10% by weight, as well as quality competitiveness and price competitiveness. It is also considered the most appropriate. In addition, the remaining amount contains the components commonly used in cosmetic compositions in the range of about 90 to 99.9% by weight based on the total weight of the acceptable carrier. In addition, as materials commonly used, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments, and flavoring agents, etc. may be included.

That is, the cosmetic product made of the cosmetic composition containing the functional collagen composition as an active ingredient is any one formulation selected from the group consisting of skin, lotion, serum, essence, UV cream, BB cream, nutrient cream, and massage cream. It can be produced in any formulation commonly produced in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, It can be formulated into powder foundation, emulsion foundation, wax foundation, spray, etc., but it can also be prepared in the form of general emulsion formulation and solubilization formulation. Emulsion-type cosmetics include nutritional lotion, cream, and essence, and solubilization-type cosmetics may include softening lotion, but are not limited thereto.

In addition, as a separate cosmetic formulation, for example, solution, gel, solid or kneaded anhydrous product, emulsion obtained by dispersing oil phase in water phase, suspension, microemulsion, microcapsule, microgranule or ionic type (liposome), non-ionic type It may also be provided in the form of a follicular dispersion, cream, skin, lotion, powder, ointment, spray or conceal stick.

In addition, the cosmetic composition may include a fatty substance, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, Water, ionic or nonionic emulsifier, filler, metal ion sequestering and chelating agent, preservative, vitamin, blocking agent, humectant, essential oil, dye, pigment, hydrophilic or lipophilic active agent, lipid vesicle or commonly used in cosmetics It may also contain adjuvants commonly used in the field of cosmetology or dermatology, such as any other ingredient that is

In addition, the above components may be introduced in an amount generally used in the field of dermatology. That is, for example, there are cosmetics such as astringent lotion, softening lotion, nourishing lotion, various creams, essences, packs, foundations, etc. Specific formulations include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, and moisture. It includes formulations such as lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, and nutrition essence.

In addition, according to another embodiment of the present invention, the collagen composition contains 0.1 to 10% by weight of either an extract or a freeze-dried powder based on the total weight of the food composition, and the remaining amount is a food preparation Characterized in that it contains in an amount of 90 to 99.9% by weight of the carrier allowed for. In this way, the functional collagen composition extracted from the goldfish is identified as vegetable collagen because it contains abundant glycine , proline , and hydroxyproline , and this functional collagen composition can be used for preparing food compositions, When looking at the functional collagen composition based on the total weight of the food composition, it is desirable to select either extract or freeze-dried powder and maintain it within the range of 0.1 to 10% by weight, as well as quality competitiveness and price competitiveness. It is also considered the most appropriate. In addition, the remaining amount contains the components commonly used in food compositions in the range of about 90 to 99.9% by weight based on the total weight of the acceptable carrier. In addition to the active ingredients of the functional collagen composition, for example, various flavoring agents or natural carbohydrates may be included as additional ingredients, as in conventional food compositions.

That is, the food prepared from the food composition containing the functional collagen composition as an active ingredient is used as a carrier, and examples of natural carbohydrates include monosaccharides such as glucose and disaccharides such as fructose, such as maltose and sugar and polysaccharides such as dextrin, cyclodextrin, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the above flavors, natural flavors (such as thaumatin), rebaudioside A, glycyrrhizin, and the like, and synthetic flavors (such as saccharin and aspartame) can be advantageously used. The food composition may be formulated in the same way as the pharmaceutical composition and used as a functional food or added to various foods. For example, there are beverages, meat, chocolate, foods, confectionery, pizza, ramen, other noodles, chewing gum, candy, ice cream, alcoholic beverages, vitamin complexes, and health supplements.

In addition, the food composition, in addition to the functional collagen composition as an active ingredient, various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, and the like. In addition, the food composition may contain fruit flesh for preparing natural fruit juice, fruit juice beverages, and vegetable beverages.

In addition, food compositions can be manufactured and processed into functional health foods in the form of tablets, capsules, powders, granules, liquids, pills, etc. It refers to food manufactured and processed using ingredients B, and refers to intake for the purpose of obtaining useful effects for health purposes such as adjusting nutrients for the structure and function of the human body or physiological functions.

On the other hand, health functional foods may contain normal food additives, and the suitability as food additives is determined according to the general rules of food additives and general test methods approved by the Korea Food and Drug Administration, unless otherwise specified. and judged by criteria. For example, a health functional food in the form of a tablet is obtained by granulating a mixture obtained by mixing a functional collagen composition, which is an active ingredient of the present invention, with excipients, binders, disintegrants, and other additives in a conventional manner, and then adding a lubricant and compressing the mixture. Or, the mixture may be directly compression molded. Among health functional foods in the form of capsules, hard capsules can be prepared by filling a mixture of a functional collagen composition, which is the active ingredient of the present invention, with additives such as excipients, in a conventional hard capsule, and soft capsules contain a functional collagen composition. It can be prepared by filling a mixture with additives such as excipients into a capsule base such as gelatin. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a coloring agent, a preservative, and the like, if necessary. The health functional food in the form of a pill can be prepared by molding a mixture of a functional collagen composition, which is an active ingredient of the present invention, and excipients, binders, disintegrants, etc. It can be coated, or the surface can be coated with a material such as starch or talc. A health functional food in the form of a granule can be prepared in granular form by a conventionally known method of mixing a functional collagen composition, which is an active ingredient of the present invention, with excipients, binders, disintegrants, etc., and, if necessary, flavoring agents and corrigents etc. may be included.

In addition, according to another embodiment of the present invention, the collagen composition contains 0.1 to 10% by weight of either an extract or a freeze-dried powder based on the total weight of the pharmaceutical composition, and the remaining amount is a pharmaceutical It is characterized by containing in an amount of 90 to 99.9% by weight of the carrier acceptable for the formulation. In this way, the functional collagen composition extracted from the Keumhwagyu is identified as vegetable collagen as it contains abundant glycine , proline , and hydroxyproline , and this functional collagen composition can be used for the preparation of pharmaceutical compositions, When looking at the functional collagen composition based on the total weight of the pharmaceutical composition, it is desirable to select either extract or freeze-dried powder and maintain it within the range of 0.1 to 10% by weight, as well as quality competitiveness and price competitiveness. It is also considered the most appropriate. In addition, the remaining amount contains the components commonly used in pharmaceutical compositions in the range of about 90 to 99.9% by weight based on the total weight of the acceptable carrier. And, as a commonly used material, for example, in addition to the active ingredient of a functional collagen composition, it can be prepared using pharmaceutically suitable and physiologically acceptable adjuvants, such as conventional pharmaceutical compositions, and the adjuvants include excipients and disintegrants , Sweeteners, binders, coating agents, expanding agents, lubricants, lubricants, or flavoring agents may be used, and the pharmaceutical composition includes one or more pharmaceutically acceptable carriers in addition to the above-described active ingredients for administration. It can be preferably formulated into a pharmaceutical composition.

That is, the formulation form of the pharmaceutical composition is not particularly limited, and can be preferably formulated according to each disease or component using a method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field. can

In addition, the pharmaceutical composition may be a composition for external application on the skin. The pharmaceutical composition for external application to the skin is a pharmaceutical composition for external application to the skin having a skin protection effect from ultraviolet rays, such as a cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta or cataplasma. It can be prepared and used, but is not limited thereto. A preferred dosage of the pharmaceutical composition for external application to the skin varies depending on the condition and weight of the patient, the severity of the disease, the type of drug, the route and duration of administration, but can be appropriately selected by those skilled in the art.

In addition, pharmaceutical compositions can also be used for the manufacture of quasi-drug compositions. "Quasi-drugs" refer to products with milder effects than pharmaceuticals among items used for the purpose of diagnosing, treating, improving, mitigating, treating or preventing human or animal diseases. For example, according to the Pharmaceutical Affairs Act, quasi-drugs are products excluding items used for pharmaceutical purposes, including products used for the treatment or prevention of human or animal diseases, products with minor or no direct action on the human body, etc. , for example in the group consisting of body cleansers, disinfectant cleansers, detergents, kitchen cleaners, cleaning cleaners, toothpastes, gargles, wet wipes, detergents, soaps, hand washes, hair washes, hair softeners, humidifier fillers, masks, ointments and filter fillers It can be prepared in a selected formulation, but is not limited thereto.

In particular, the pharmaceutical composition of the present invention can also be manufactured into capsules. On the other hand, in the recent hard capsule industry, demand has shifted from animal to vegetable capsules, and the production volume of vegetable capsules is rapidly increasing. Since vegetable capsules are 70-80% more expensive than animal-based capsules, demand for collagen is rising due to high profitability. The global vegetable capsules market is valued at 115.1 million US$ in 2020 and is expected to reach 229.6 million US$ by the end of 2026, growing at a CAGR of 10.0% during 2021-2026.

These vegetarian capsules are natural, GMO, vegetable shells and are free of animal derivatives, gluten and modified sugars. Vegetarian capsules today can be divided into several types: plant polysaccharide ( Pululan ), starch and HPMC ( Hydroxyl Propyl Methyl Celluloses ). Vegetarian capsules have the advantage of being suitable for halal or kosher certification. It has a pure, clean and elegant appearance without taste or smell, ideal for improving the bioavailability of useful ingredients, easy to swallow, safe, non-carcinogenic, and tamper-evident, so there is no difficulty in using it.

In this way, the present invention completely acquires collagen amino acids obtained from Keumhwagyu in the most efficient way, thereby solving the weak points of animal collagen, while improving joint health, skin aging and wrinkles. It can be used as a variety of raw materials for cosmetic raw materials, functional food raw materials, etc., as well as free formulation changes can be easily pursued, and in particular, collagen amino acid (GPH; glycine, proline), a component similar to skin cells that helps collagen synthesis in functional and organic aspects , Hydroxyproline) content is very high, so the absorption rate in the body is fast, and it is involved in the amount of skin moisture, preventing skin aging, weakening of joints and blood vessel damage when ingested, as well as moisturizing the skin and improving wrinkles when applied, providing excellent product preservation and safety. .

The present invention is not limited to the described embodiments, and it is obvious to those skilled in the art that various modifications and variations can be made without departing from the spirit and scope of the present invention. Therefore, such variations or modifications should fall within the scope of the claims of the present invention.

Claims (5)

After cultivating Aurea Helianthus , select any one of the shade-dried leaves, stems, and roots, wash them to remove foreign substances, dry them in the shade at 35 to 45 ° C, and then grind them using a grinder, and 1 kg of the pulverized product Using 6L of 20% alcohol, extract for 2 to 5 days in a shaking incubator at 150 RPM and 15 ℃, and filter the plant 4 to 5 times through a 1mm × 1mm thick filter net due to the high collagen content of the extract. After removing the residue, it was concentrated under reduced pressure with an evaporator at 30 ~ 40 ℃ to remove 20% alcohol, and then distilled water was added to obtain a final 4L, 2.9 ~ 3.0% Brix extract. Frequency) 20kHz, Amplitude (Amplitude) 60%, glycine ( Glycin ), Proline ( Proline ), hydroxyproline ( hydroxyproline ), separated by sonication for 3 to 4 hours under the condition of 4 ℃, extracted from collagen amino acid Next, use any one of cosmetic, food , and pharmaceutical compositions for joint health, skin aging, and wrinkle improvement containing an active ingredient of collagen amino acid of any one of the above glycine, proline , and hydroxyproline A functional collagen composition using a gold-derived collagen amino acid, characterized in that. delete According to claim 1,
The collagen composition is a cosmetic composition, and contains 0.1 to 10% by weight of either a collagen amino acid extract or freeze-dried powder based on the total weight of the cosmetic composition, and the remaining amount is a carrier acceptable for cosmetic formulations 90 A functional collagen composition using collagen amino acids derived from goldfish, characterized in that it is contained in an amount of ~ 99.9% by weight. According to claim 1,
The collagen composition is a food composition, and contains 0.1 to 10% by weight of either a collagen amino acid extract or freeze-dried powder based on the total weight of the food composition, and the remaining amount is a carrier acceptable for food formulations 90 A functional collagen composition using collagen amino acids derived from goldfish, characterized in that it is contained in an amount of ~ 99.9% by weight. According to claim 1,
The collagen composition is a pharmaceutical composition for joint health, skin aging and wrinkle improvement, and contains 0.1 to 10% by weight of either a collagen amino acid extract or a freeze-dried powder based on the total weight of the pharmaceutical composition, and the remaining amount ) Functional collagen composition using collagen amino acid derived from goldfish, characterized in that it is contained in an amount of 90 to 99.9% by weight of a carrier acceptable for pharmaceutical formulations.

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