KR102488136B1 - Cosmetic composition comprising an apricot extract and soluble collagen - Google Patents

Abstract

It relates to a cosmetic composition, which contains an apricot extract and collagen, and can be usefully used for preventing, improving or treating skin-related conditions.

Description

Cosmetic composition comprising an apricot extract and soluble collagen

It relates to a cosmetic composition.

Skin aging is divided into intrinsic aging that occurs naturally and extrinsic aging caused by external factors. Extrinsic aging is the cause of aging from the outside, and may be affected by ultraviolet rays, environmental pollution, etc., and can be prevented through various methods such as sunscreen and face washing. Endogenous aging occurs naturally according to genetic factors and aging, and frequently occurs not only inside the body but also in external tissues such as the skin.

The main symptoms of skin aging include wrinkles and loss of elasticity, which is known to be caused by a decrease in collagen and elastin, which are main proteins in the dermis, and a network deformation of these elastic fibers. Collagen, elastin, and fibronectin present in the dermis and epidermis form a dense network to maintain the skin cushion layer, but as aging reduces the number of collagen fibers and fragments and twists them, the density of the elastic fiber network decreases. Loss of normal skin support.

Collagen synthesis genes are involved in collagen synthesis, and MMPs (matrix metalloproteinases) that degrade connective tissue components including collagen exist. As collagen synthesis decreases with aging and the activity of MMPs enzymes that decompose collagen increases, collagen degradation increases, and many studies have been reported that wrinkles increase as a result of reducing elasticity. As such, collagen fibers are an essential substance for improving skin aging.

Apricot (Prunus armeniaca) is a plant belonging to the genus Rosaceae and is called a passerby in oriental medicine. In particular, herbal external preparations made by grinding apricot seeds are used for skin pigmentation such as spots and freckles, swelling, swelling, etc., and make the skin white and shiny.

Recently, interest in health functional foods and inner beauty products such as edible fish collagen and edible hyaluronic acid is increasing. In particular, as consumers' interest in anti-aging among skin care costs increases, interest in clean and safe collagen raw materials that can appeal for anti-aging efficacy and can replace chemical and animal-derived materials is increasing.

One aspect is to provide a cosmetic composition containing apricot extract and water-soluble collagen as active ingredients.

One aspect provides a composition comprising apricot extract and water-soluble collagen as active ingredients.

As used herein, the term "collagen" is a type of protein and constitutes bone, skin, cartilage, connective tissue, etc. depending on the type. Numerous collagen synthesis genes are involved in collagen synthesis, and matrix metalloproteinases (MMPs) that degrade connective tissue components including collagen exist. Aging reduces collagen synthesis and increases the activity of MMPs enzymes that decompose collagen, which increases the degradation of collagen, reducing elasticity and increasing wrinkles. The collagen is composed of various amino acids, such as glycine, proline, and alanine. In one embodiment, the collagen may be water-soluble collagen, and may be isolated from fish, hyaluronic acid, mammals (pig, cow, chicken, etc.), yeast, fruit, vegetable, mushroom, etc. In addition, the water-soluble collagen may be commercially available. In addition, the collagen may have a molecular weight of 300 Da to 100 KDa. For example, the collagen has a molecular weight of, for example, 300 Da to 100 KDa, 300 Da to 50 KDa, 300 Da to 10 KDa, 500 Da to 1 KDa, 700 Da to 1 KDa or 1 KDa to 100 KDa. it could be At this time, when the size of the molecular weight of collagen exceeds the above range, there is a problem in that it is not efficiently absorbed into the body.

The apricot extract is obtained by extracting apricots with water, C1 to C4 lower alcohol, or a mixture thereof as a solvent, and the apricot extract may be obtained by extracting apricot flesh, seeds, or a mixture thereof. In extraction, after cutting the dried apricots, water, alcohol or a mixture thereof may be added in an amount of 2 to 20 times the weight of the apricots for extraction. The extraction may be performed at a temperature of 20 to 100 °C for 1 to 10 hours. As the extraction method, cold extraction, ultrasonic extraction, or reflux cooling extraction method may all be used, and extraction may be repeated 1 to 5 times.

In one embodiment, it was confirmed that the composition containing the apricot extract and water-soluble collagen increased the content of anti-aging and anti-wrinkle factors, such as COL1A1, or decreased the expression of MMP-1, which was increased by ultraviolet light. In particular, it was confirmed that the composition containing the apricot extract and water-soluble collagen showed a synergistic effect of increasing COLA1A content or decreasing MMP-1 expression compared to a composition containing the apricot extract or water-soluble collagen alone. Therefore, the composition may have a synergistic skin beauty improvement effect, for example, skin aging inhibition, skin barrier strengthening, skin wrinkle inhibition, or skin moisturizing effect, when separately containing apricot extract or water-soluble collagen.

In one embodiment, the composition may be for improving the skin condition or improving skin beauty. For example, the skin condition or skin beauty improvement may be suppression or improvement of skin aging, anti-wrinkle, skin elasticity, skin regeneration, skin barrier strengthening, or skin moisturizing.

As used herein, the term "skin aging" refers to both tangible and intangible changes that appear in the skin with age, such as thinning of the epidermis, number of cells or blood vessels in the dermis, DNA damage repair ability, cell replacement cycle , wound healing, skin barrier function, epidermal moisture retention, sweat secretion, sebum secretion, vitamin D production, physical damage defense, chemical substance elimination ability, immune response, sensory function, and decrease in body temperature control. In particular, wrinkles and loss of elasticity are the main symptoms of skin aging, which are known to be caused by the reduction of collagen and elastin, which are the main proteins in the dermis, and the network deformation of these elastic fibers. Elastin and fibronectin, which exist in the dermis and epidermis, form a dense network to maintain the skin cushion layer. loses its holding power.

The composition may be used to improve skin aging caused by exogenous factors or endogenous factors. The extrinsic factor refers to various external factors, such as ultraviolet rays (light), and the endogenous factor is also referred to as a chronological factor and refers to a factor mainly caused by the passage of time. That is, the skin aging is specifically a natural aging phenomenon that occurs as the proliferation of skin cells decreases with age as well as premature aging symptoms induced by external stimuli such as ultraviolet rays, pollution, cigarette smoke, chemicals, etc. It is a concept that includes wrinkles, loss of elasticity, skin sagging and dryness. In addition, wrinkles include stimulation caused by changes in internal and external factors that cause wrinkles by changing components constituting skin tissue.

The aging may be photoaging. As used herein, the term "photoaging" is a phenomenon caused by external environmental factors, and the most representative factor is ultraviolet rays. Ultraviolet rays cause damage to biocomponents such as activation of proteolytic enzymes, chain scission of matrix proteins, and abnormal cross-linking, and repetition of these mechanisms results in apparent skin aging.

As used herein, the term "wrinkle" refers to a state in which elasticity of the skin is lost and loosened, and for example, the skin may be folded. The "prevention or improvement of skin wrinkles" may mean any action of preventing or improving wrinkles by suppressing the expression of wrinkle-related factors or increasing the total amount of collagen.

The "strengthen the skin barrier" may refer to any action that enhances the function of the skin barrier, which is located at the outermost layer of the skin and prevents loss of moisture and nutrients. In addition, "damaged skin barrier function" may refer to all changes in the skin due to a decrease or damage to the function of the skin barrier. Examples include increased skin wrinkling, dryness, dermatitis, atopic dermatitis, allergic dermatitis, acne, and the like.

The "moisturizing skin" may refer to any action that maintains skin moisture or prevents moisture loss.

In one embodiment, the apricot extract may be included in 0.01 to 99% by weight based on the total weight of the composition. The apricot extract is, for example, 0.01 to 99% by weight, 1 to 95% by weight, 1 to 90% by weight, 5 to 90% by weight, 5 to 80% by weight, 10 to 70% by weight, 40% by weight based on the total weight of the composition to 99% by weight, or 20 to 70% by weight. At this time, when the content of the apricot extract is less than the above range, there is a problem that the apricot extract cannot sufficiently improve the skin condition, and when it exceeds the above range, the active ingredients in the apricot extract are deteriorated or the process cost is increased. there is.

In addition, the water-soluble collagen may be included in an amount of 0.01 to 99% by weight based on the total weight of the composition. The water-soluble collagen is, for example, 0.01 to 99% by weight, 1 to 95% by weight, 1 to 90% by weight, 5 to 90% by weight, 5 to 80% by weight, 10 to 70% by weight, 40% by weight based on the total weight of the composition to 99% by weight, or 20 to 70% by weight. At this time, when the content of water-soluble collagen is less than the above range, there is a problem that the effect of improving skin conditions cannot be sufficiently exhibited, and when it exceeds the above range, the viscosity is high and the texture is sticky.

In addition, the apricot extract and water-soluble collagen may be mixed in a weight ratio of 1:0.1 to 99. The apricot extract and water-soluble collagen are, for example, 1:0.1 to 99, 1:1 to 99, 1:10 to 90, 1:10 to 95, 1:10 to 80, 1:5 to 95, 1:25 to 75, 1:30 to 70, 1:35 to 65, 1:40 to 60, 1: 0.1 to 2 or 1:1.

In one embodiment, the composition may be a cosmetic composition.

The cosmetic composition may have, for example, softening lotion, nutrient lotion, massage cream, nutrient cream, essence, pack, gel, ampoule, or skin-adhesive cosmetic formulation.

Ingredients included in the cosmetic composition may include ingredients commonly used in cosmetic compositions other than the composition as active ingredients, for example, conventional adjuvants and carriers such as stabilizers, solubilizers, vitamins, pigments and flavors. can include

In another embodiment, the composition may be a composition for external application for skin.

The external skin preparation may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, or a combination thereof. The external skin preparation is a component usually used in external preparations for skin such as cosmetics or pharmaceuticals, for example, water-based components, oil-based components, powder components, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, and fragrances. , colorants, various skin nutrients, or combinations thereof and may be suitably blended as needed. The external skin preparations include metal sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, bellapamil, licorice extract, glabridin, and calin. Hot-water extracts of fruits, various herbal medicines, tocopherol acetate, glycyrrhizic acid, tranexamic acid and its derivatives or salts and other drugs, vitamin C, magnesium ascorbic acid phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose , saccharides such as trehalose, etc. can also be blended appropriately.

In another embodiment, the composition may be a health functional food composition.

The health functional food composition may be used alone or in combination with other foods or food ingredients, and may be appropriately used according to conventional methods. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, when preparing food or beverage, the composition of the present specification may be added in an amount of 15 parts by weight or less based on the raw material. There is no particular limitation on the type of health functional food. Among the types of health functional foods, beverage compositions may contain various flavoring agents or natural carbohydrates as additional components, like conventional beverages. The natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as thaumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used. The health food composition may also contain nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, and carbonated beverages. carbonation agent used, or a combination thereof. The health functional food composition may also contain natural fruit juice, fruit juice beverages, fruit flesh for preparing vegetable beverages, or a combination thereof.

Another aspect provides a method for preventing, improving or treating a skin condition in a subject comprising treating or administering to a subject in need thereof an effective amount of the composition. Details of the skin condition are as described above.

As used herein, the terms "administering," "introducing," and "implanting" are used interchangeably and according to one embodiment into a subject by a method or route that results in at least partial localization of a composition to a desired site. It may mean the arrangement of the composition according to one embodiment of.

Administration may be administered by a method known in the art. Administration can be administered directly to a subject by any means, for example, by routes such as intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal or subcutaneous administration. can The administration may be administered systemically or locally.

The subject may be a mammal, such as a human, cow, horse, pig, dog, sheep, goat, or cat. The subject may be a subject in need of skin beauty improvement, for example, skin moisturizing, skin barrier strengthening, and skin wrinkle improvement effects.

The administration is 0.1 mg to 1,000 mg per day of the composition according to one embodiment, for example, 0.1 mg to 500 mg, 0.1 mg to 100 mg, 0.1 mg to 50 mg, 0.1 mg to 25 mg, 1 mg to 1,000 mg, 1 mg to 500 mg, 1 mg to 100 mg, 1 mg to 50 mg, 1 mg to 25 mg, 5 mg to 1,000 mg, 5 mg to 500 mg, 5 mg to 100 mg, 5 mg to 50 mg , 5 mg to 25 mg, 10 mg to 1,000 mg, 10 mg to 500 mg, 10 mg to 100 mg, 10 mg to 50 mg, or 10 mg to 25 mg may be administered. However, the dosage may be prescribed in various ways depending on factors such as formulation method, administration method, patient's age, weight, sex, pathological condition, food, administration time, administration route, excretion rate and reaction sensitivity, and those skilled in the art can Dosage can be appropriately adjusted in consideration of these factors. The number of administrations can be once a day or twice or more within the range of clinically acceptable side effects, and the administration site can be administered to one or more than two sites, daily or every 2 to 5 days, total The number of administration days may be administered from 1 day to 30 days per treatment. If necessary, the same treatment can be repeated after a titration period. For non-human animals, the same dosage per kg as for humans is used, or the above dosage is converted by the volume ratio (eg, average value) of the organ (heart, etc.) between the target animal and the human. A single dose can be administered.

A composition according to one aspect contains an apricot extract and collagen, so it can be usefully used for preventing, improving or treating skin-related conditions.

1 is a graph showing the effect of a cosmetic composition according to one embodiment on the expression of MMP-1.
Figure 2 is a graph showing the effect of the cosmetic composition according to one embodiment on the expression of COL1A1.

Hereinafter, a preferred embodiment is presented to aid understanding of the present invention. However, the following examples are provided to more easily understand the present invention, and the content of the present invention is not limited by the following examples.

[Example]

Examples 1-5. Preparation of a Composition Comprising Apricot Extract and Water-Soluble Collagen

The apricots were naturally dried for 240 hours and then washed. Thereafter, an apricot extract was prepared using dried apricots with water at 50 ° C. The yeast was washed with water and mixed in a 0.1N NaOH solution for about 12 hours to remove protein impurities. Yeast from which impurities were removed was obtained, washed with distilled water, and put in 10% butyl alcohol for about 24 hours to remove the fatty part. The remaining residue was removed and placed in a solution containing 0.5 M acetic acid and 10% (w/v) pepsin for 24 to 48 hours. Thereafter, the solution was filtered or centrifuged to obtain a supernatant. Water-soluble collagen was prepared by adding NaCl to the obtained supernatant to separate and permeate only the precipitate. A composition containing the apricot extract and water-soluble collagen was prepared by mixing the apricot extract and water-soluble collagen obtained from yeast in a weight ratio shown in Table 1 below. Unless otherwise stated in this specification, content is in weight percent.

Example 1 Example 2 Example 3 Example 4 Example 5 Apricot Extract 50 10 30 70 90 water soluble collagen 50 90 70 30 10

[Comparative example]

Comparative Example 1. Preparation of apricot extract

An apricot extract was prepared in the same manner as in the above example.

Comparative Example 2. Preparation of water-soluble collagen

Water-soluble collagen was prepared in the same manner as in the above example.

[Experimental example]

anti-aging activity

The effect of the cosmetic composition of Example 1 on factors related to skin aging and wrinkle formation was analyzed.

Specifically, a human dermal fibroblast cell line (Hs68) was cultured in DMEM (Dulbecco's modified Eagle's medium) containing 10% fetal bovine serum (FBS) and 1% penicillin, and the culture was carried out at 37°C, 5% CO ₂ and It was performed for 24 hours in an incubator at 95% humidity. Thereafter, the medium was removed, DPBS was added, and 20 mJ/cm 2 of UVB was irradiated or not irradiated. Immediately after UVB irradiation, DPBS was removed and replaced with FBS-free medium, and then Example 1 and Comparative Examples 1 and 2 were treated by concentration (5ppm, 10ppm) and further cultured for 24 hours. Pure distilled water was added as a negative control group and 1 µM EGCG was added as a positive control group. Thereafter, cells in each medium were obtained, washed with cold PBS, and RNA was isolated using an RNAprep kit (Monarch®Total RNAprep Kit, NEB, USA), RNA was quantified at 260 nm with nanodrop, and each 2 μg cDNA was synthesized in an amplifier using the RNA of (C1000 Thermal Cycler, Bio-Rad, USA). Using the synthesized cDNA as a template, cybergreen (SYBR Green supermix, Applied Biosystems, USA) was added together with primers (see Table 2 below) and cDNA for the target genes COL1A1 and MMP-1, followed by real-time PCR. Real-time polymerase chain reaction was performed on a machine (Step One Plus, Applied Biosystems, USA). The real-time PCR reaction was performed by repeating 40 cycles of polymerase activation at 94°C for 5 minutes, followed by 95°C for 30 seconds, 55°C for 30 seconds, and 72°C for 30 seconds. The expression levels of the COL1A1 and MMP-1 genes were finally analyzed through correction for the β-actin gene, and the results are shown in FIGS. 1 and 2, respectively.

gene forward primer reverse primer COL1A1 5′-GAGGGCCAAGACGAAGACATC-3′ 5'-CAGATCACGTCATCGCACAAC-3' MMP-1 5′-CGAATTGCCGACAGAGATGA-3′ 5′-GTCCCTGAACAGCCCAGTACTT-3′ β-actin 5′-GGCCATCTCTTGCTCGAAGT-3′ 5′-GAGACCTTCAACACCCCAGC-3′

Figure 1 is a graph showing the effect of the cosmetic composition according to one embodiment on the expression of MMP-1, Figure 2 is a graph showing the effect of the cosmetic composition according to one embodiment on the expression of COL1A1.

As a result, as shown in FIG. 1, it can be confirmed that the apricot extract and water-soluble collagen decrease the UVB-induced MMP-1 expression in a concentration-dependent manner. Specifically, in the case of Example 1 containing the apricot extract and water-soluble collagen at a concentration of 5 ppm, respectively, it can be seen that the expression of MMP-1 is significantly reduced compared to Comparative Examples 1 and 2 at the same concentration. In addition, it was confirmed that the expression of MMP-1 was remarkably reduced when treated with Example 1 compared to when the concentration of Comparative Examples 1 and 2 was treated twice (10 ppm), respectively. In addition, in the case of Examples 2 to 5 containing the apricot extract and water-soluble collagen in different amounts, it was confirmed that the expression of MMP-1 was significantly reduced compared to Comparative Examples 1 and 2.

In addition, as shown in Figure 2, it was confirmed that the apricot extract and water-soluble collagen significantly increased the expression of COL1A1 in a concentration-dependent manner. Specifically, in the case of Example 1, it was confirmed that the expression of COL1A1 significantly increased compared to when Comparative Examples 1 and 2 were treated at the same concentration and twice (10 ppm) concentration. In addition, in the case of Examples 2 to 5 containing the apricot extract and the water-soluble collagen in different amounts, it was confirmed that the expression of COL1A1 significantly increased compared to Comparative Examples 1 and 2.

That is, the composition according to one aspect may exhibit a synergistic effect in reducing collagen degradation and increasing collagen synthesis when the apricot extract and the water-soluble collagen are treated in combination compared to when the apricot extract and the water-soluble collagen are treated separately.

The above description of the present invention is for illustrative purposes, and those skilled in the art can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, the embodiments described above should be understood as illustrative in all respects and not limiting.

Claims (6)

A cosmetic composition for improving skin condition or moisturizing, comprising an apricot extract and water-soluble collagen isolated from fish, hyaluronic acid, mammals, yeast, fruits, vegetables, or mushrooms as active ingredients. The cosmetic composition according to claim 1, wherein the composition reduces the expression of MMP-1 or increases the expression of COL1A1. The cosmetic composition according to claim 1, wherein the skin condition is skin aging, skin barrier enhancement, skin wrinkles, skin elasticity, or skin regeneration. The cosmetic composition according to claim 1, wherein the apricot extract and water-soluble collagen are mixed in a weight ratio of 1:0.1 to 10. A composition for topical skin application for improving skin conditions or moisturizing, comprising an apricot extract and water-soluble collagen isolated from fish, hyaluronic acid, mammals, yeast, fruits, vegetables, or mushrooms as active ingredients. A health functional food composition for improving skin condition or moisturizing, comprising an apricot extract and water-soluble collagen isolated from fish, hyaluronic acid, mammals, yeast, fruits, vegetables, or mushrooms as active ingredients.

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