KR102447268B1 - Manufacturing method of brown mealworm larvae powder - Google Patents

Abstract

The present invention relates to a method for producing Tenebrio molitor larvae powder. More specifically, the method includes: a washing step of washing Tenebrio molitor larvae; a larvae heating step of heating the larvae washed through the washing step; an epidermal separation step of separating the epidermis of the larvae heated through the larvae heating step; a drying step of drying the larvae whose epidermis has been removed through the epidermal separation step; and a crushing step of grinding the larvae dried through the drying step. The Tenebrio molitor larva powder prepared through the above process not only contains various nutrients in abundance, but also shows excellent marketability because the epidermis of the larvae is separated. By heating, pressing and drying the larvae, the odor peculiar to insects is reduced. Thus, the Tenebrio molitor larvae powder can be used as simple feed, pet food raw material, meat substitute, food additive and health functional food.

Description

Manufacturing method of brown mealworm larvae powder {MANUFACTURING METHOD OF BROWN MEALWORM LARVAE POWDER}

The present invention relates to a method for producing brown mealworm larvae powder, and more particularly, not only contains abundant various nutrients, but also exhibits excellent marketability by separating the larvae's epidermis, and heating, compressing and drying the larvae It relates to a method for producing brown mealworm larvae powder, which can be used as sweet feed, pet food raw material, meat substitute, food additive, and health functional food because its characteristic odor is reduced.

Simple feed is a vegetable, animal or mineral material that is used directly as feed or as a raw material for compounded feed.

On the other hand, aquaculture refers to the cultivation and harvesting of aquatic organisms necessary for human life in water as in land agriculture, also called aquatic agriculture. Korea is surrounded by the sea on three sides, and the sea characteristics such as the water temperature and topography of the east and west coasts are different, so it is possible to cultivate various kinds of fish depending on the sea area. .

However, in the current domestic aquaculture industry, due to rapid quantitative expansion, environmental factors caused by aggravation of water pollution due to indiscriminate sprawl of farms and excessive use of various disinfectants such as formalin and hydrogen peroxide, and the introduction of new fish species. Due to biological factors such as inflow and appearance and emergence of resistant bacteria due to the abuse of various antibiotics, sudden death occurs throughout the year as well as high temperature, causing serious damage to many aquaculture farms.

Currently, antibiotic therapy is mainly used for prevention and treatment of diseases occurring in fish farms, but the misuse of antibiotics causes serious problems such as emergence of resistant bacteria and contamination of the surrounding water.

On the other hand, recently, efforts are being made to search for useful substances from insects and use them as resources. In particular, insects have a very efficient and economical advantage in terms of resource use due to their short generation and small size. In addition, insects have low water loss and a hard and light body structure, so their activity energy efficiency is high, and there is no competition between larvae and adults due to the different feeding requirements between larvae and adults. It has a strong self-defense ability against physical obstacles or external intrusion hospitals, so it has many advantages in the search for useful substances compared to other organisms.

On the other hand, brown mealworm (scientific name: Tenebrio molitor) is a kind of insect belonging to the mealworm family. It is similar to grain mealworm, but is larger in larvae, and is often called mealworm and edible insect called highworm. Mealworm brown is a domestic species, and only the American mealworm (superworm) is an exotic species. Brown mealworm is distributed all over the world including Korea. It is a fully metamorphic insect that goes through the egg → larva → pupa → adult stage and was approved as a general food ingredient by the Ministry of Food and Drug Safety in 2016.

Brown mealworm in its natural state is known to develop one generation throughout the year, but it is possible to develop multiple generations throughout the year depending on the breeding temperature. They mainly overwinter in the form of larvae and pupate the following year, then emerge and reproduce. The lifespan of adults is 50 to 160 days, and it is generally known as 60 to 90 days, and the spawning period is 22 to 137 days.

Among the total fat of brown mealworm, the ratio of unsaturated fat, which helps blood vessel health, was more than 3/4. According to the Korea Food Communication Forum (KOFRUM), Professor Kim Ae-jeong's team at the Graduate School of Alternative Medicine at Kyonggi University analyzed the fat of brown mealworm larvae by type. The results of this study were introduced in the recent issue of the Journal of the Korean Society for Food and Nutrition (Misook Kang, Min-ju Kim, Han-sun Han, and Ae-jeong Kim. (2017) Fatty acid composition and anti-inflammatory effect of freeze-dried brown mealworm larvae. Journal of the Korean Society of Food and Nutrition, 30(2), 251-256 ). In the paper, the research team said, "Compared to the 55.9% unsaturated fat ratio in beef, the brown mealworm larvae have a very high unsaturated fat ratio." There is,” he introduced.

The content of palmitic acid (a type of saturated fat) known to cause an inflammatory reaction in the fat of freeze-dried brown mealworm larvae was as low as 16.8 g per 100 g, while the content of oleic acid (a type of unsaturated fat) known to inhibit the inflammatory reaction was 42.1. g as high.

Oleic acid is known to lower blood cholesterol levels and lower the risk of cardiovascular diseases such as heart disease and stroke. In addition, it was found that freeze-dried brown mealworm larvae extract inhibited the inflammatory factors TNF (tumor necrosis factor)-α (alpha) and IL (interleukin)-6 through the study. The research team pointed out in the paper, "Brown mealworm larvae are an excellent food supply of unsaturated fat, which is beneficial to blood vessel health, and an edible insect that is expected to have an anti-inflammatory effect."

As for the studies on Mealworm of Brown in Korea, there have been reports of studies using Mechlouria extract, such as the cytotoxic effect of the Mealworm larvae extract on liver cancer, and the antibacterial effect of the antifungal protein isolated from Mealworm from Korea.

Currently, the processing method of brown mealworm larvae is manufactured in a relatively simple way by washing, drying and pulverizing brown mealworm larvae. As a result, it can only be added to foods and health functional foods in a limited form.

The outer wall of the brown mealworm larva is made of a-chitin to protect itself. Chitin binds to protein and lowers the digestibility, and chitin is a tough and indigestible component. In addition, there is a fact that chitin induces accumulation in the tissues of innate immune cells related to allergies. It is stipulated that the label must be labeled that it may cause an allergy to crustaceans, and this chitin is a component of the shells of shrimp, clams, crayfish, and crabs, and it is indigestible and very hard, so people eat it after peeling it.

In addition, when powdering of brown mealworm larvae with the existing insect processing method is performed, due to the insect's unique smell and taste, a sense of distance is felt and it is very inconvenient when ingested.

Due to the above-listed problems, it is currently mainly used as pet food, the development of food using brown mealworm powder is slow, and the reality is that there is a limit to its use due to the rejection of brown mealworm powder.

Korean Patent Registration No. 10-1025810 (Mar. 23, 2011) Korean Patent Registration No. 10-1743734 (2017.05.30.)

The object of the present invention is not only to contain various nutrients abundantly, but also to show excellent marketability by separating the epidermis of the larvae, and to reduce the peculiar smell of insects by heating, pressing and drying the larvae, so it is a raw material for simple feed and pet food. , to provide a method for producing brown mealworm larvae powder that can be used as a meat substitute, food additive, and health functional food.

An object of the present invention is a washing step of washing the brown mealworm larvae, a larval heating step of heating the larvae washed through the washing step, an epidermis separation step of separating the epidermis of the larvae heated through the larva heating step, the epidermis separation It is achieved by providing a method for producing brown mealworm larvae powder, characterized in that it comprises a drying step of drying the larvae from which the epidermis has been removed through the steps and a grinding step of pulverizing the larvae dried through the drying step.

According to a preferred feature of the present invention, the washing step is performed by immersing the brown mealworm larvae in purified water at 25 to 35° C. for 20 to 30 hours, and then irradiating 30 to 90 kHz of ultrasonic waves for 30 to 60 minutes.

According to a more preferred feature of the present invention, between the epidermal separation step and the drying step, the larvae from which the epidermis has been removed through the epidermal separation step are sterilized at a temperature of 70 to 80 ° C. A hydrolysis step of mixing 0.1 to 0.5 parts by weight of a hydrolase and hydrolysis at a temperature of 60 to 70° C. for 20 to 30 hours is further performed.

According to a more preferred feature of the present invention, the hydrolase is selected from the group consisting of alkarease, pepsin, trypsin, alpha chymotrypsin, papain, bromelain, nutrase, protamex, flavorzyme and pancrease. It shall consist of one or more selected.

According to an even more preferred feature of the present invention, the larva heating step is to be made by putting the larvae washed through the ultrasonic washing step into purified water of 90 to 100 ℃ and heating for 10 to 20 minutes.

According to an even more preferred feature of the present invention, the larva heating step is to be made by heating the larvae washed through the ultrasonic washing step with steam at a temperature of 100 to 120 ° C. for 20 to 30 minutes.

According to an even more preferred feature of the present invention, the epidermal separation step is made by removing the separated epidermis after press-compressing or screw-compressing the larvae washed through the washing step.

According to an even more preferred feature of the present invention, the drying step shall consist of freeze-drying or spray-drying.

According to an even more preferred feature of the present invention, the freeze-drying is after quenching the larvae from which the epidermis has been removed through the epidermal separation step to a temperature of -45 to -40 °C, and then the quenched larvae to a temperature of 30 to 40 °C. It is supposed to consist of a process of drying for 20 to 30 hours.

The method for producing brown mealworm larvae powder according to the present invention not only contains various nutrients abundantly, but also shows excellent marketability by separating the larva's epidermis, and by heating, pressing and drying the larvae, the odor peculiar to insects is reduced. Therefore, it has an excellent effect of providing brown mealworm larvae powder that can be used as sweet rice feed, pet food raw material, meat substitute, food additive, and health functional food.

1 is a flowchart showing a method for producing brown mealworm larvae powder according to an embodiment of the present invention.
Figure 2 is a flow chart showing a method for producing brown mealworm larvae powder according to another embodiment of the present invention.
Figure 3 is a photograph showing the state of the brown mealworm larvae washed during the process of Example 1 of the present invention by putting them in purified water and heating, and screw-compressing the heated larvae.
4 is a photograph showing the chitinous epidermis separated through press compression during the process of Example 1 of the present invention.
5 is a photograph showing the components of the lyophilized brown mealworm larvae during the process of Example 1 of the present invention.
6 is a photograph showing the brown mealworm larvae powder prepared in Example 1 of the present invention.

Hereinafter, a preferred embodiment of the present invention and the physical properties of each component will be described in detail, which is intended to describe in detail enough that a person of ordinary skill in the art to which the present invention pertains can easily carry out the invention, This does not mean that the technical spirit and scope of the present invention is limited.

The method for producing brown mealworm larvae powder according to the present invention includes a washing step (S101) of washing the brown mealworm larvae, a larva heating step of heating the larvae washed through the washing step (S101) (S103), the larva heating step ( Through the epidermal separation step (S105) of separating the epidermis of the heated larvae through S103), the drying step (S107) of drying the larva from which the epidermis has been removed through the epidermal separation step (S105) and the drying step (S107) It consists of a grinding step (S109) of grinding the dried larvae.

The washing step (S101) is a step of washing the brown mealworm larvae, which may be washed with running water or washed with a bubble washer, etc., but washing using ultrasonic waves is more preferable.

The method of washing brown mealworm larvae using ultrasound is made by immersing brown mealworm larvae in purified water at 25 to 35° C. for 20 to 30 hours, and then irradiating 30 to 90 kHz of ultrasonic waves for 30 to 60 minutes.

In the ultrasonic washing process as described above, if the temperature of the hot water is less than 25 ℃ or the irradiation time of ultrasonic waves is less than 30 minutes, the effect of removing foreign substances remaining on the surface of the brown mealworm larvae is insignificant, and the temperature of the hot water in the ultrasonic washing process is When the temperature exceeds 35°C or the irradiation time of ultrasonic waves exceeds 60 minutes, the effect of removing foreign substances remaining on the surface of the brown mealworm larvae is not greatly improved and it is not preferable in terms of energy efficiency.

As described above, through the process of immersing the larvae in purified water, the feces of the fasted larvae can be reliably removed to ensure safety and remove the harmful elements, and the removed feces will sink in the purified water.

In addition, it can be removed to foreign substances remaining on the surface of the larvae through the ultrasonic cleaning consisting of the above process.

The brown mealworm larvae contain phenolic compounds, and the phenolic compounds contain phenolic hydroxyl groups (-OH) as one of the tea metabolites widely distributed in plants, and are easily combined with proteins and macromolecules. has a character Phenolic compounds exhibit various functions such as antioxidant effects, and as the content of phenolic compounds increases, antioxidant activity also tends to increase. Total phenolic compounds content was measured using extracts of brown mealworm larvae by ethanol concentration. The total phenolic compounds content of the ethanol (50% mass concentration) extract was 459.23±1.05 mg%, which was significantly highest among the extracts, and the water extract was 362.62±3.62 mg% and the 70% mass concentration ethanol extract was 335.75±6.96 mg. %, mass concentration 95% ethanol extract was in the order of 103.82±0.84 mg%.

In addition, brown mealworm larvae contain flavonoids, and flavonoids are one of the naturally occurring compounds among phenolic compounds. They have a property of binding to proteins and macromolecules, and exhibit antioxidant effects by acting as a reducing agent, singlet oxygen quencher, and hydrogen donor. Total flavonoid content was measured using extracts of lyophilized brown mealworm larvae for each ethanol concentration. The total flavonoids content of the water extract was the highest at 19.86±0.69 mg%, the mass concentration of 95% ethanol extract was 16.85±0.79 mg%, the mass concentration 70% ethanol extract was 8.44±0.00, and the mass concentration 50% ethanol extract was 8.33±0.15 mg% was shown.

As a result of the above, it was confirmed that the brown mealworm larvae have a high content of antioxidants and thus excellent antioxidant activity, and it can be seen that the value of use as functional materials such as antioxidants is high.

In addition, brown mealworm larvae contain physiologically active peptides, which are small in size and can be easily absorbed into the body, and have the advantage of having various functional properties. The effects of physiologically active peptides studied so far include improving the antioxidant function of milk protein hydrolyzate in the body, preventing oxidation in processed foods, and a new antibacterial peptide from egg white hydrolyzate. , immunomodulation, and other functional studies of physiologically active peptides are being actively conducted, and the production of physiologically active peptides through enzymatic hydrolysis of protein-rich brown mealworm larvae is attracting attention.

In addition, brown mealworm larvae contain a large amount of linoleic acid, which shows the efficacy of preventing hair loss or promoting hair growth.

Hair loss refers to excessive hair loss due to a prolonged resting phase or an increase in the number of hair follicles in the resting phase during the hair growth cycle. Although there are hair loss treatments such as minoxidil and finasteride, minoxidil can cause side effects such as allergic dermatitis, itching, and hair loss when discontinuing use.

From 131% (0.1 mg/ml) to 151% of the brown mealworm larva extract treated for 24 hours, and from 203% (0.1 mg/ml) to 218% (2 mg/ml) when the extract was treated for 48 hours up to the cell proliferation effect. This was a result similar to or higher than the growth rate of dermal papilla cells confirmed using brier root, red ginseng and hwangchil tree leaves, which were found to have a hair growth effect. Therefore, it is expected that the brown mealworm larva extract can help hair growth by promoting the proliferation of dermal papilla cells.

About 28% of apoptosis was induced in dermal papilla cells treated with DHT alone, but the cell viability increased significantly from 0.1 mg/ml concentration when treated with the brown mealworm larvae extract compared to the group treated with DHT alone, 0.5 From the mg/ml concentration, cell viability was similar to that of normal cells not treated with DHT. Therefore, it is expected that the brown mealworm larva extract can prevent hair loss by inhibiting the cell death of dermal papilla cells caused by DHT.

In addition, it has been reported that the brown mealworm larva extract exhibits nitrite scavenging ability, which is related to the content of polyphenols such as flavonoids and caffeic acid in food, so that it can also show antioxidant effects through nitrite scavenging ability. As a positive control group in the nitrite scavenging activity assay, blueberry extract (10 mg/ml), which was reported to have antioxidant effect, was used, and the nitrite scavenging ability of the blueberry extract was found to be 40.36%. As a result of analyzing the nitrite scavenging ability of the brown mealworm larvae extract by concentration, it showed nitrite scavenging activity of 6.26% at 1 mg/ml, 26.16% at 5 mg/ml, and 43.69% at 10 mg/ml. confirmed that. In addition, the brown mealworm larvae extract 10 mg/ml shows a higher nitrite scavenging ability than the blueberry extract (10 mg/ml), which is known to have high antioxidant activity, so it is expected to be used as a natural preservative and anti-oxidation agent. .

In addition, it was confirmed that the oil component contained in Mealworm larvae increased the ALP activity of MG-63 osteoblasts and thus the differentiation of osteoblasts was increased. It means that it can be used as a material that can be given.

As a result of confirming the expression of ALP protein, the protein expression level increased from 40 μg/ml to a significant difference from the CTR group, and an expression level similar to that of the osteoblast differentiation induction medium (DM) used as a positive control could be observed. In addition, at a concentration of 80 μg/ml, the expression level of ALP protein was increased about 4 times compared to CTR.

In addition, the brown mealworm larva extract exhibits anticancer properties, and the cancer cell-selective cytotoxic activity was demonstrated by in vitro and in vivo experiments targeting cancer cell lines. First, the ethanol extract of brown mealworm larvae had little toxicity to primary hepatocytes, which can be called normal cells, but also had cytotoxicity to cancer cell lines and other normal cells, primary cardiomyocytes. The ethanol extract was further fractionated with a liquid-liquid partition using hexane, butanol, ethyl acetate and water, and the components were separated. Among these fractions, the hexane fraction was used for various cancer cells (PC3, 22Rv1, HeLa, PLC/PRF5, HepG2, Cardiomyocyte toxicity was significantly reduced while maintaining toxicity to Hep3B, SK-HEP-1, HCT116, NCI-H460, MDA-MB231, SKOV3). Although the 0.4 mg/mL ethanol extract showed the toxicity of killing most cardiomyocytes, the hexane fraction showed only about 20% cytotoxicity under the same conditions, confirming that the toxicity was significantly reduced. It was confirmed that this non-specific cytotoxicity can be reduced by material separation and fractionation. Second, it was confirmed that hexane and ethyl acetate fractions can activate representative cell death mechanisms such as apoptosis, apoptosis, and autophagy. In addition, it was confirmed that the cell death-inducing activity of the hexane fraction can increase the anticancer activity when it is treated with a liver cancer cell line together with anticancer substances widely prescribed in clinical practice. Based on these experimental results, it was confirmed that the extracts of brown mealworm larvae could have anticancer activity either alone or in combination with other cytotoxic drugs. Finally, the anticancer activity of the hexane fraction was confirmed in an in vivo xenograft mouse model. In mice transplanted with the liver cancer cell line SK-HEP-1, intraperitoneal injection of the hexane fraction for 15 days significantly inhibited tumor growth. It was confirmed, and consistent with the previous limited effect on normal cells, symptoms that could be called side effects such as weight loss were not confirmed.

Combining the above results, the anticancer activity of the brown mealworm larva extract could be confirmed in vitro and in vivo, and additional fractionation and material analysis are needed to discover new substances with anticancer activity.

The larva heating step (S103) is a step of heating the larvae washed through the washing step (S101), and the brown mealworm larvae washed through the washing step (S101) are put into purified water at 90 to 100 ° C. It can be made by heating for 20 minutes, or by heating the brown mealworm larvae washed through the washing step (S101) with steam at a temperature of 100 to 120° C. for 20 to 30 minutes.

When the larvae heating step (S103) consisting of the above process is performed, the phenomenon of rancidity of unsaturated fatty acids contained in the larvae is suppressed due to the pressing, etc. proceeding in the epidermal separation step (S105), thereby exhibiting excellent marketability, preservation and preference. , microorganisms and enzymes are removed, so it is possible to provide larvae powder suitable for pet food raw materials, meat substitutes, food additives and health functional foods as well as aquaculture fish feed.

If the process of epidermis separation is immediately proceeded to separate the shell from the brown mealworm larvae, rancidity due to the unsaturated fatty acid component contained in the larvae occurs rapidly, so after washing the larvae, the larvae through the larva heating step (S103) Inhibits rancidity caused by unsaturated fatty acids.

At this time, when the larva heating step (S103) consists of a process of putting the larva into purified water of 90 to 100 ° C and heating for 10 to 20 minutes, if the heating temperature is less than 90 ° C or the heating time is less than 10 minutes, the larvae Because of the unsaturated fatty acids contained in it, rancidity proceeds, and when the heating temperature exceeds 100°C or the heating time exceeds 20 minutes, the heating process is continuously carried out in a situation where the rancidity of the unsaturated fatty acids is suppressed. It is undesirable because the nutrients contained in the larvae are excessively destroyed.

In addition, when the larva heating step (S103) consists of a process of heating the larva with steam at a temperature of 100 to 120 ° C. for 20 to 30 minutes, if the temperature of the steam is less than 100 ° C. or the heating time is less than 20 minutes, the larvae Because of the unsaturated fatty acids contained in it, rancidity proceeds, and when the steam temperature exceeds 120℃ or the heating time exceeds 30 minutes, the heating process is continuously carried out in a situation where the rancidity of the unsaturated fatty acids is suppressed, so in terms of energy efficiency It is undesirable because the nutrients contained in the larvae are excessively destroyed.

At this time, in the larva heating step (S103), additives such as licorice, garlic, apple, onion and peppercorns may be added as necessary to reduce the taste and smell peculiar to insects in the process of heating the larvae. It is preferable that 1 to 5 parts by weight of the additive comprising the above components are added relative to 100 parts by weight of the larvae washed through step (S101).

The epidermis separation step (S105) is a step of separating the epidermis of the larva heated through the larva heating step (S103). After pressing or screwing the brown mealworm larva heated through the larva heating step (S103) , it consists of a process of removing the separated epidermis. When the epidermis of the larva is removed through the above process, the chitin component remaining in the epidermis of the oil layer is removed and the FCR performance (feed requirement) is not reduced. In addition to showing the effect of enhancing immunity, survival rate and weight gain, it is possible to provide brown mealworm larvae powder suitable as pet food raw materials, meat substitutes, food additives and health functional foods.

The epidermis of brown mealworm larvae consists of alpha chitin, and the chitin content is 17.58g based on g/100g.

Due to the content of chitin, hydrolysis technology using insect powder is being developed, but the barrier to entry is very high due to the high material cost and mechanical equipment cost of using yeast during hydrolysis, and the unique difficulties caused by the use of yeast when using hydrolysis technology There was a big downside to the presence of fragrance.

Hydrolysis is the process of breaking down proteins into amino acids, which are the skeletons. The amino acid groups do not cause allergic reactions, but because of their large particles, they create high osmotic pressure. Therefore, it is not well absorbed in the intestine and cannot be used directly in the diet.

In addition, if the hydrolyzed protein is fed for a long time, nutritional deficiency and imbalance may occur, and the current technology does not hydrolyze 100% of the protein to proceed with the hydrolysis using yeast. Due to these problems, there have been several attempts to use the larvae powder of brown mealworm in food, but there was a problem with low marketability due to the unique taste and smell of brown mealworm, discomfort when ingested, and allergic reaction. Therefore, the epidermis of larvae with a high content of alpha chitin must be essentially removed.

The brown mealworm larvae powder produced through the present invention removes the chitinous shell and is manufactured using only the internal components of the larvae, so it is comfortable to swallow when ingested, is well dispersed in water, and does not infringe on the taste of existing foods and foods. effect can be shown.

The drying step (S107) is a step of drying the brown mealworm larva from which the epidermis has been removed through the epidermal separation step (S105), and freeze-drying or spray-drying the larva from which the epidermis has been removed through the epidermal separation step (S105). made by the process

The freeze-drying is performed by rapidly cooling the brown mealworm larva from which the epidermis has been removed through the epidermal separation step (S105) to a temperature of -45 to -40°C, and then cooling the quenched larva to a temperature of 30 to 40°C for 20 to 30 hours. It consists of a drying process.

At this time, the freeze-drying is carried out for 4 hours at a temperature condition of 30° C. and a vacuum degree of 5000 torr, then at a temperature condition of 35 to 40° C. and a vacuum degree of 800 Mtorr for 12 to 22 hours, and a temperature condition of 30° C. and a vacuum degree of 800 Mtorr. It is most preferable to proceed for 4 hours at a vacuum degree. When the freeze-drying step is performed through the above temperature conditions, time and vacuum degree, the destruction of nutrients or proteins contained in the brown mealworm larvae is minimized. Drying of the freeze-drying step If the temperature exceeds 50 ℃, it is not preferable because the nutrients or protein components contained in the larvae are destroyed.

In addition, the moisture contained in the larvae freeze-dried for the above temperature and time is sublimated to further improve the preservation.

In addition, the spray drying is not particularly limited as long as it is a device and condition of spray drying that can be used in the manufacturing process of feed, and any spray drying can be used. However, spray-drying is faster and more automated than the freeze-drying, and there is an advantage in that it is convenient because no manpower is input.

In addition, it is preferable that the spray drying consists of a process of putting the larvae from which the epidermis has been removed through the epidermal separation step (S105) into a spray dryer and spray-drying for 10 to 15 minutes using a microwave.

The moisture content of the larvae from which the epidermis was removed through the epidermis separation step (S105) was about 70%, and the larvae from which the epidermis was removed through the epidermis separation step (S105) were put into a spray dryer equipped with a microwave irradiation device and 70 When spray-drying for 10 to 15 minutes at a temperature of 80 ° C., the content of water contained in the larva is lowered, and the content of essential amino acids or proteins is improved.

At this time, if the drying temperature during the spray drying is less than 70 ℃, the efficiency of the drying process is reduced, and when the drying temperature during the spray drying exceeds 80 ℃, it is not preferable because the protein contained in the larva may be destroyed.

Since the larvae spray-dried for the above temperature and time are reduced in weight by 1/4 to 1/3 due to the removal of moisture, when 3 to 4 kg of the larvae are dried, about 1 kg of larvae powder can be produced.

In addition, when spray-drying is performed as described above, the solubility of the protein contained in the larvae is about 40%, and the process such as neutralization does not proceed, thereby providing larval powder with improved digestibility.

The grinding step (S109) is a step of pulverizing the larvae dried through the drying step (S107), and the larvae dried through the drying step (S107) are pulverized using a general grinder or an ultra-fine grinder. .

In addition, between the epidermal separation step (S105) and the drying step (S107), the brown mealworm larva from which the epidermis has been removed through the epidermal separation step (S105) is irradiated with microwaves and sterilized at a temperature of 70 to 80 ° C. A hydrolysis step (S106) of mixing 0.1 to 0.5 parts by weight of hydrolase in 100 parts by weight of sterilized brown mealworm larvae and hydrolysis at a temperature of 60 to 70° C. for 20 to 30 hours may be further performed, under the above conditions The hydrolyzed larvae can be provided as larvae powder that further improves the effects of strengthening immunity, survival rate and growth rate of not only farmed fish but also pets by further micronizing nutrients or protein particles.

In this case, the hydrolase is formed by mixing at least one selected from the group consisting of alkarease, pepsin, trypsin, alpha chymotrypsin, papain, bromelain, nutrase, protamex, flavorzyme and pancrease. Preferably, the hydrolase composed of the above components can further improve the hydrolysis efficiency of nutrients or proteins contained in brown mealworm larvae.

Hereinafter, the method for producing the brown mealworm larvae powder according to the present invention and the physical properties of the larvae powder prepared by the manufacturing method will be described with reference to examples.

<Example 1>

Brown mealworm larvae (fasted for more than 3 days) were immersed in purified water at 30° C. for 24 hours, then washed by irradiating 60 kHz ultrasonic waves for 45 minutes, and the washed larvae were put into purified water and heated at a temperature of 95° C. for 15 minutes. Then, the epidermis was separated by screw-pressing the heated larvae, and the larva from which the epidermis was separated was quenched to a temperature of -42.5 ° C. It was dried for 17 hours under temperature conditions and a vacuum degree of 800 Mtorr, dried for 4 hours under a temperature condition of 30 ° C. and a vacuum degree of 800 Mtorr), and the dried larvae were put into a grinder and pulverized to prepare brown mealworm larvae powder.

The brown mealworm larvae powder prepared in Example 1 shows 612.04 Kcal per 100 g, and contains 1.05% dietary fiber, about 41.81% crude protein, 45.74% crude fat, 3.10% raw flour, and 0.54% water.

The nutritional components contained in the brown mealworm larvae powder prepared in Example 1 were analyzed and shown in Table 1 below.

<Table 1>

As shown in Table 1, the brown mealworm larvae powder prepared in Example 1 of the present invention contains a large amount of nutrients.

In addition, the brown mealworm larvae powder prepared in Example 1 of the present invention was photographed and shown in FIG. 6 below. As shown in Figure 6 below, it can be seen that the brown mealworm larvae powder prepared in Example 1 of the present invention is prepared as a powder in the form of fine particles by removing the chitinous epidermis.

Therefore, the method for producing brown mealworm larvae powder according to the present invention not only contains various nutrients abundantly, but also shows excellent marketability by separating the larvae's epidermis, and by heating, pressing and drying the larvae, there is no smell peculiar to insects. It provides brown mealworm larvae powder that can be used as sweet rice feed, pet food raw material, meat substitute, food additive, and health functional food.

S101; washing step
S103; Larva heating stage
S105 ; epidermal separation step
S106 ; hydrolysis step
S107; drying stage
S109; crushing step

Claims (9)

A washing step of washing the brown mealworm larvae;
Larvae heating step of heating the larvae washed through the washing step;
an epidermis separation step of separating the epidermis of the larvae heated through the larva heating step;
a drying step of drying the larva from which the epidermis has been removed through the epidermal separation step; and
A grinding step of pulverizing the dried larvae through the drying step; consists of,
The washing step is made by immersing the brown mealworm larvae in purified water at 25 to 35° C. for 20 to 30 hours, and then irradiating 30 to 90 kHz of ultrasonic waves for 30 to 60 minutes,
The larva heating step is made by putting the larvae washed through the washing step into purified water of 90 to 100 ℃ and heating for 10 to 20 minutes, or the larvae washed through the washing step into steam at a temperature of 100 to 120 ℃ A method for producing brown mealworm larvae powder, characterized in that it is heated for 20 to 30 minutes.
delete The method according to claim 1,
Between the epidermal separation step and the drying step, the larvae from which the epidermis has been removed through the epidermal separation step are sterilized at a temperature of 70 to 80 ° C. A method for producing brown mealworm larvae powder, characterized in that a hydrolysis step of mixing parts by weight and hydrolysis at a temperature of 60 to 70° C. for 20 to 30 hours is further performed.
4. The method of claim 3,
The hydrolase is alkarease, pepsin, trypsin, alpha chymotrypsin, papain, bromelain, nutrase, protamex, flavorzyme, characterized in that consisting of one or more selected from the group consisting of pancreases Method for producing brown mealworm larvae powder.
delete delete The method according to claim 1,
The epidermis separation step is a method for producing brown mealworm larvae powder, characterized in that after press-pressing or screw-compressing the larvae washed through the washing step, and then removing the separated epidermis.
The method according to claim 1,
The drying step is a method for producing brown mealworm larvae powder, characterized in that consisting of freeze-drying or spray-drying.
9. The method of claim 8,
The freeze-drying is a process of rapidly cooling the larvae from which the epidermis has been removed through the epidermal separation step to a temperature of -45 to -40°C, and then drying the quenched larvae at a temperature of 30 to 40°C for 20 to 30 hours. Method for producing brown mealworm larvae powder, characterized in that.

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