KR102430399B1 - A composition for improving, preventing and treating of gastrointestinal disease - Google Patents

Abstract

The present invention relates to a composition for preventing, treating or improving gastric diseases caused by Helicobacter pylori, comprising: a mixed extract obtained by extracting a mixture consisting of milk thistle and dandelion; cabbage extract; honey; ginger juice; and stevia as an active ingredient, so it has an excellent effect on gastric diseases caused by Helicobacter pylori, and is non-toxic, so it can be consumed in the form of food. It is possible to improve, prevent or treat gastric diseases caused by pylori.

Description

The composition for preventing, treating or ameliorating gastric diseases caused by Helicobacter pylori containing a mixture consisting of milk thistle, dandelion, cabbage, honey and ginger

The present invention relates to a composition for preventing, treating or ameliorating gastric diseases caused by Helicobacter pylori containing a mixture consisting of milk thistle, dandelion, cabbage, honey and ginger.

Digestive diseases include liver-related diseases such as hepatitis and cirrhosis, and stomach and intestine-related diseases such as colitis, colorectal cancer, gastroenteritis, gastric cancer, functional gastrointestinal disorders, and enteritis. It has not been reduced.

In particular, the causes of gastric diseases can be broadly classified in detail, such as factors caused by bacterial diseases such as Helicobacter pylori, physical factors such as stress, and chemical factors such as ethanol.

The Helicobacter pylori mainly infects the gastrointestinal mucosa and is a direct cause of acute gastritis, chronic gastritis, gastric ulcer, duodenal ulcer, and gastric lymphoma. It is known that environmental factors and genetic factors act to increase the incidence of gastric adenocarcinoma. Therefore, Helicobacter pylori has been warned as a primary cancer-causing agent by the World Health Organization (WHO) in 1994.

Helicobacter pylori infection is very common among Koreans, with an infection rate of 46.6% of the total population and 69.4% of adults. It is speculated that such a high rate of Helicobacter pylori is the cause of the high incidence of gastric adenocarcinoma in Koreans. Antibiotics are used to treat Helicobacter pylori, and the commonly used first-line treatment for Helicobacter pylori consists of three types of drugs, including two antibiotics and one gastric acid inhibitor. If you take it for 7-14 days, there is a 70-80% chance of curing Helicobacter pylori. However, recently, as the antibiotic resistance of Helicobacter pylori has increased, there are more and more cases where the bacteria do not disappear even after treatment, which is a problem. In addition, interest in the development of food or natural product extracts is increasing. In particular, according to the Maastricht III consensus report in 2007, it was mentioned that probiotics may be a tool for treating Helicobacter pylori infection.

In addition, it has been reported that co-administration of lactic acid bacteria during the primary eradication treatment of Helicobacter pylori using antibiotics can improve the eradication rate and reduce complications caused by the use of antibacterial agents [Sun Hee Roh. 2011. The Effect of Probiotics for Helicobacter pylori. The Korean Journal of Helicobacter and Upper Gastrointestinal Research. 11(1); 26-36].

In order to continuously treat gastric diseases caused by Helicobacter pylori, there is a need for a therapeutic agent using a natural product that has fewer side effects than chemical drugs and has secured stability.

Republic of Korea Patent No. 1902932 Republic of Korea Patent No. 1984129

It is an object of the present invention to provide a food composition that can prevent or improve gastric diseases caused by Helicobacter pylori by containing a mixture consisting of milk thistle, dandelion, cabbage, honey and ginger.

Another object of the present invention is to provide a method for preparing a food composition capable of preventing or improving gastric diseases caused by Helicobacter pylori.

In addition, another object of the present invention is to provide a food composition capable of preventing or treating gastric diseases caused by Helicobacter pylori by containing a mixture consisting of milk thistle, dandelion, cabbage, honey and ginger.

The food composition for preventing or improving gastric diseases caused by Helicobacter pylori of the present invention for achieving the above object is a mixed extract obtained by extracting a mixture consisting of milk thistle and dandelion; cabbage extract; honey; ginger juice; And it may contain a mixture consisting of stevia as an active ingredient.

The mixed extract may be an extract extracted at 80 to 100° C. by mixing milk thistle and dandelion in a weight ratio of 1: 0.2-1.

1 to 30 parts by weight of cabbage extract, 0.5 to 10 parts by weight of honey, 0.1 to 5 parts by weight of ginger juice, and 0.1 to 5 parts by weight of stevia based on 100 parts by weight of the mixed extract may be mixed.

The gastric disease caused by Helicobacter pylori may be one or more gastric diseases selected from the group consisting of acute gastritis, chronic gastritis, gastric ulcer, duodenal ulcer, gastric cancer and gastric lymphoma caused by Helicobacter pylori.

The thistle may be a mixture of flowers, stems and leaves, and roots of thistles in a weight ratio of 1:14-25:0.1-0.9.

In addition, the method for preparing a food composition for the prevention or improvement of gastric diseases caused by Helicobacter pylori for achieving the above other object is (A) obtaining a mixed extract by mixing and extracting milk thistle and dandelion; (B) adding and mixing cabbage extract and ginger juice to the mixed extract; and (C) mixing by adding honey and stevia to the mixture of the cabbage extract and ginger juice.

In addition, the pharmaceutical composition for the prevention or treatment of gastric diseases caused by Helicobacter pylori of the present invention for achieving the above another object is a mixed extract obtained by extracting a mixture consisting of milk thistle and dandelion; cabbage extract; honey; ginger juice; And it may contain a mixture consisting of stevia as an active ingredient.

The composition for preventing, treating or improving gastric diseases caused by Helicobacter pylori of the present invention is selected from the group consisting of acute gastritis, chronic gastritis, gastric ulcer, duodenal ulcer, gastric cancer and gastric lymphoma by inhibiting the growth of Helicobacter pylori. It is possible to prevent, treat or improve gastric diseases caused by one or more Helicobacter pylori bacteria, and since it is non-toxic, it can be consumed in the form of food.

The present invention relates to a composition for preventing, treating or ameliorating gastric diseases caused by Helicobacter pylori containing a mixture consisting of milk thistle, dandelion, cabbage honey and ginger.

The gastric disease caused by Helicobacter pylori may be one or more gastric diseases selected from the group consisting of acute gastritis, chronic gastritis, gastric ulcer, duodenal ulcer, gastric cancer and gastric lymphoma caused by Helicobacter pylori.

Hereinafter, the present invention will be described in detail.

The composition for the prevention, treatment or improvement of gastric diseases caused by Helicobacter pylori of the present invention is a mixed extract obtained by extracting a mixture consisting of milk thistle and dandelion; cabbage extract; honey; ginger juice; and a mixture consisting of stevia as an active ingredient.

thistle

The thistle used in the present invention may be a white thistle (Silybum marianum (L.) Gaertn.), a spiny thistle (Cirsium japonicum Fisch ex DC. var. spinossimum Kitam.), or a mixture thereof, and preferably a white thistle. Thistle alone is used.

The white patterned thistle (Silybum marianum (L.) Gaertn.) is a deep, shiny green plant with milky white leaf veins, and a wavy edge at the edge. It is mainly found on levee fences and abandoned lands. The useful parts of the plant are the whole herb, roots, leaves, seeds, and husks, and thistle seeds contain a flavonoid compound known as silymarin. Thistle seed extract contains 70-80% silymarin.

The thistle according to the present invention is a mixed extract containing a mixture of flowers, stems and leaves, and roots of thistle.

The flower of the thistle has a purple or red corolla and a length of 19-24 mm. It is preferable to use only those with a length of 10 to 15 mm and not use those with a longer length than that for toxicity.

In addition, the thistle stem and leaves are used by mixing in a weight ratio of 1: 1-4, preferably 1: 2-3. If the content of the leaf based on the stem is less than the lower limit, the bitter taste may be strong, and if it exceeds the upper limit, it is not preferable for the prevention, treatment or improvement of gastric diseases caused by Helicobacter pylori.

In addition, unlike flowers, stems and leaves, which are harvested twice a year, the thistle root is harvested once every three years, so it is preferable to use it after drying.

The flower, stem and leaf mixture, and root of the thistle are mixed in a weight ratio of 1:14-25:0.1-0.9, preferably 1:16-19:0.4-0.8 by weight. If the content of the mixture of stems and leaves and roots based on the flower is less than the lower limit, the effect on the prevention, treatment or improvement of gastric diseases caused by Helicobacter pylori may not be excellent, and if the upper limit is exceeded It has a strong bitter taste, and the effect on the prevention, treatment or improvement of gastric diseases caused by Helicobacter pylori may be reduced.

The milk thistle mixed extract of the present invention is a hot water extract extracted at 80 to 100° C. for 20 to 30 hours, preferably 24 to 28 hours. If the extraction temperature and time is less than the lower limit, the active ingredient may not be extracted, and if it exceeds the upper limit, the burnt taste may be strong and the nutrients may be destroyed. In addition, when extraction is performed by other methods such as organic solvent extraction other than boiling water extraction using water, the effect on the prevention, treatment or improvement of gastric diseases caused by Helicobacter pylori is insignificant, and a strong bitter taste may occur. .

Specifically, the mixed extract of thistle is extracted by mixing a mixture of flowers, stems and leaves, and separately extracting the roots in a bath and mixing them later. Unlike the present invention, in the case of extracting by mixing all the mixtures and roots of flowers, stems and leaves and roots, the bitter taste is strong and the effect on the prevention, treatment or improvement of gastric diseases caused by Helicobacter pylori may be reduced. .

Specifically, the method for preparing this mixed extract of thistle includes the steps of: (a) mixing a mixture of flowers, stems and leaves of thistle, and extracting the extract with hot water at 80 to 100°C; (b) extracting the root of thistle at 80 to 100° C. to obtain an extract; and (c) lyophilizing the extracts obtained in steps (a) and (b), respectively, to powder them and mixing them. In addition, after step (c), (d) drying and pulverizing the residue remaining after extraction in step (b); and (e) mixing 100 parts by weight of the powder mixed in step (c) and 0.1 to 3 parts by weight of the powder of step (d); may further include.

In contrast to steps (a) and (b), when extracting by mixing all of the flowers, stems and leaves, and roots, the bitter taste is strong and, rather, the prevention, treatment or improvement of gastric diseases caused by Helicobacter pylori. may be less effective for; In the case of extracting flowers, a mixture of stems and leaves, and roots, respectively, in a bath, the yield may be reduced.

When the root residue powder prepared in step (D) is added, the effect on disease can be significantly improved compared to the case where the root residue powder is not added.

dandelion

The dandelion (Taraxacum platycarpum) is also called Geumjamcho, Jijeong, Pogongyeong, Pogongcho, Anjilbang, and also has a nickname called Satseunbang. The leaves or stems contain proteins, carbohydrates, fats, ash, minerals, vitamins, etc. evenly. Young shoots are used as greens or soup. In oriental medicine, the roots and the outpost before flowering are said to have antipyretic, anti-inflammatory, diuretic, and gastric effects. use it

In the present invention, milk thistle and dandelion are mixed in a weight ratio of 1:0.2-1, preferably 1:0.4-0.6 by weight.

If the content of dandelion based on milk thistle is less than the lower limit, the effect of preventing, treating or improving gastric diseases caused by Helicobacter pylori may be lower than when each substance is used alone, and if it exceeds the upper limit, There may be little effect in preventing, treating or ameliorating gastric diseases caused by Helicobacter pylori.

The mixed extract mixed with thistle and dandelion of the present invention is obtained by mixing the mixture of milk thistle and dandelion and the extraction solvent in a weight ratio of 1:1 to 100, preferably 1:5 to 20, and 40 to 50 at 80 to 100 ° C. Extract over time to prepare an extract. When the weight ratio of the mixture and the extraction solvent is out of the above range, the active ingredient of the milk thistle and dandelion mixture may be extracted in a small amount in the extract.

The extraction solvent for extracting the extract is water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The extraction solvent is not particularly limited, but the extract extracted with water shows an excellent effect.

Cabbage Extract

The cabbage (Cabbage) has thick leaves, no hair, turns white like powder, has irregular serrations on the edges, and overlaps with each other, and the innermost leaves are round and hard like a ball, which is effective in helping health, preventing cancer, It has the effect of improving blood circulation, detoxification, and constipation.

The cabbage extract is used in an amount of 1 to 30 parts by weight, preferably 5 to 15 parts by weight, based on 100 parts by weight of the mixed extract. If the content of cabbage extract is less than the lower limit, the effect of preventing, treating or improving gastric diseases caused by Helicobacter pylori may be reduced. The effect of preventing, treating, or ameliorating gastric diseases may be lower than when each substance is used alone.

The cabbage extract of the present invention is prepared by mixing cabbage and an extraction solvent in a weight ratio of 1:1 to 100, preferably 1: 5 to 20, at 80 to 100° C. for 8 to 15 hours, preferably for 10 to 12 hours. It is prepared by hot water extraction. When the weight ratio of the mixture and the extraction solvent is out of the above range, the active ingredient of cabbage may be extracted in a small amount in the extract.

The extraction solvent for extracting the extract is water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The extraction solvent is not particularly limited, but the extract extracted with water shows an excellent effect.

ginger juice

The ginger is a rhizome, which grows sideways, is fleshy, has a lumpy shape, is yellow, and has a pungent taste and a fragrant smell. Ginger treats chills, fever, headache, vomiting, seawater, and phlegm caused by a cold. As pharmacological action, gastric juice secretion promotion, digestive power enhancement, cardiac excitatory action, blood circulation promotion, and antibacterial action have been reported.

In the present invention, ginger juice from which solids are removed after squeezing ginger is used, and when prepared as an extract and used, the effect of preventing, treating or improving gastric diseases caused by Helicobacter pylori may be reduced.

In addition, the ginger juice has little effect on the prevention, treatment or improvement of gastric diseases caused by Helicobacter pylori when used alone, but when used with the mixed extract of thistle and dandelion and cabbage extract, ginger juice alone or the mixed extract It shows 1.5 to 20 times better efficacy than when used alone.

The ginger juice is used in an amount of 0.1 to 5 parts by weight, preferably 0.5 to 2 parts by weight, based on 100 parts by weight of the mixed extract. When the content of ginger juice is less than the lower limit, the effect of preventing, treating, or improving gastric diseases caused by Helicobacter pylori may be reduced, and if it exceeds the upper limit, the sensory properties may be reduced.

honey

The honey has no effect when used alone, but when used with the mixed extract of thistle and dandelion, cabbage extract and ginger juice, it is also effective in preventing, treating or improving gastric diseases caused by Helicobacter pylori.

The honey is used in an amount of 0.5 to 10 parts by weight, preferably 2 to 6 parts by weight, based on 100 parts by weight of the mixed extract. If the content of honey is less than the lower limit, there may be no effect of preventing, treating, or improving gastric diseases caused by Helicobacter pylori, and if it exceeds the upper limit, the sensory properties may be reduced and the manufacturing cost may be improved.

Stevia

The stevia ( Stevia rebaudiana ) is growing around rivers or wetlands, and has been using stevia leaves as a sweetener since ancient times. The leaves contain stevioside, a sweetener, about 6-7% by weight, and the content varies greatly depending on the individual. The sweetening ingredient is 300 times that of sugar, and it is used as a sweetener in soft drinks, as a substitute for tea or chewing gum.

In the present invention, by using stevia, the sensory decreased by the use of ginger juice is increased.

The stevia is used in an amount of 0.1 to 5 parts by weight, preferably 0.2 to 1 parts by weight, based on 100 parts by weight of the mixed extract. When the content of stevia is out of the above range, the functionality may be reduced.

In addition, the present invention provides a method for preparing a composition for preventing, treating or improving gastric diseases caused by Helicobacter pylori.

The method for preparing a composition for preventing, treating or improving gastric diseases caused by Helicobacter pylori of the present invention comprises the steps of (A) obtaining a mixed extract by mixing and extracting milk thistle and dandelion; (B) adding and mixing cabbage extract and ginger juice to the mixed extract; and (C) mixing by adding honey and stevia to the mixture of the cabbage extract and ginger juice.

First, in step (A), a mixture of milk thistle and dandelion is extracted with an extraction solvent to obtain a mixed extract.

The mixing conditions, extraction solvent and extraction conditions of the mixture are the same as those mentioned above.

Next, in step (B), cabbage extract and ginger juice are added to the mixed extract at room temperature (23-27° C.) to prepare a mixture of cabbage extract and ginger juice.

When the mixed extract is prepared by mixing thistle and dandelion in step (A), by adding cabbage extract and ginger juice separately later, rather than extracting by adding cabbage extract and ginger juice, the sensory function is increased and the stomach caused by Helicobacter pylori. The effect of preventing, treating or ameliorating a disease may be further improved. When extraction is performed by adding cabbage and ginger to milk thistle and dandelion, the prevention, treatment or improvement effect of gastric diseases caused by Helicobacter pylori is not improved compared to the case where cabbage and ginger are not added. It is preferable to separately add cabbage extract and ginger juice to one mixed extract.

Next, in step (C), honey and stevia are added to the mixture of the cabbage extract and ginger juice at room temperature and mixed.

The honey and stevia are added after the cabbage extract and ginger juice are sufficiently mixed with the mixed extract, and when the cabbage extract, ginger juice, honey and stevia are simultaneously added and mixed, the sensory properties may be significantly reduced.

The form of the composition of the present invention may be prepared in the form of a gel, puree, juice, pills, large pills and granules.

For example, in the case of being prepared in the form of gel and puree, the mixture of cabbage extract and ginger juice prepared in step (B) is concentrated to 1/3 to 1/5 of the volume of the mixture with a concentrator. Step (C) is performed using the concentrate. That is, after mixing the concentrate with honey and stevia, agar is added, stirred, and then sterilized to prepare gel and puree.

As another example, when it is prepared in the form of juice, the mixture prepared in step (C) is sterilized and used as it is.

As another example, when it is prepared in the form of rings, large rings and granules, the mixture of cabbage extract and ginger juice prepared in step (B) is mixed with a concentrator in a volume of 1/5 to 1/7 compared to the volume of the mixture. After concentration, honey is added to the concentrate, aged for 40 to 50 hours in a water bath at 60 to 70° C., and then dried and molded to prepare pills, large rings and granules.

the mixed extract of the present invention; cabbage extract; honey; ginger juice; And Stevia; A mixture consisting of a mixed extract in a broad sense; cabbage extract; honey; ginger juice; and stevia; a mixed product formulated to be administered to an animal, such as a mixed extract; cabbage extract; honey; ginger juice; and stevia; has a meaning including a mixed powder of stevia. Although in the present invention the mixed extract; cabbage extract; honey; ginger juice; And Stevia; Although the experiment was conducted with a mixture consisting of, a mixed extract; cabbage extract; honey; ginger juice; and Stevia; it will be expected by those skilled in the art that the desired effect can be achieved even in the form of a mixed product.

On the other hand, in the present specification, the term 'contained as an active ingredient' means a mixed extract; cabbage extract; honey; ginger juice; and stevia; in an amount sufficient to achieve the efficacy or activity of the mixture consisting of. For example, the mixed extract; cabbage extract; honey; ginger juice; and stevia; is used at a concentration of 10 to 1500 μg/ml, preferably 100 to 1000 μg/ml. mixed extract; cabbage extract; honey; ginger juice; and stevia; a mixed extract included in the composition of the present invention because there is no side effect to the human body even when administered in excess as a natural product; cabbage extract; honey; ginger juice; and stevia; the upper limit of the quantity may be selected by those skilled in the art within an appropriate range.

The pharmaceutical composition of the present invention may be prepared by using a pharmaceutically suitable and physiologically acceptable adjuvant in addition to the active ingredient, and the adjuvant includes an excipient, a disintegrant, a sweetener, a binder, a coating agent, a swelling agent, a lubricant, A lubricant or flavoring agent may be used.

The pharmaceutical composition may be preferably formulated as a pharmaceutical composition by including one or more pharmaceutically acceptable carriers in addition to the active ingredients described above for administration.

Formulations of the pharmaceutical composition may be granules, powders, tablets, coated tablets, capsules, suppositories, solutions, syrups, juices, suspensions, emulsions, drops or injectable solutions. For formulation in the form of, for example, tablets or capsules, the active ingredient may be combined with an orally, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. In addition, if desired or necessary, suitable binders, lubricants, disintegrants and color-developers may also be included in the mixture. Suitable binders include, but are not limited to, starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tracacanth or sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like.

In the composition formulated as a liquid solution, acceptable pharmaceutical carriers are sterile and biocompatible, and include saline, sterile water, Ringer's solution, buffered saline, albumin injection, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostats may be added as needed. In addition, diluents, dispersants, surfactants, binders and lubricants may be additionally added to form an injectable formulation such as an aqueous solution, suspension, emulsion, etc., pills, capsules, granules or tablets.

Furthermore, it can be preferably formulated according to each disease or component using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA by an appropriate method in the art.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and in the case of parenteral administration, it may be administered by intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc., preferably oral administration. .

A suitable dosage of the pharmaceutical composition of the present invention varies depending on factors such as formulation method, administration mode, age, weight, sex, pathological condition, food, administration time, administration route, excretion rate and reaction sensitivity of the patient, An ordinarily skilled physician can readily determine and prescribe a dosage effective for the desired treatment or prophylaxis. According to a preferred embodiment of the present invention, the daily dose of the pharmaceutical composition of the present invention is 0.001-10 g/kg.

The pharmaceutical composition of the present invention is prepared in unit dosage form by formulating using a pharmaceutically acceptable carrier and/or excipient according to a method that can be easily carried out by a person of ordinary skill in the art to which the present invention pertains. or may be prepared by inclusion in a multi-dose container. In this case, the formulation may be in the form of a solution, suspension, or emulsion in oil or aqueous medium, or may be in the form of an extract, powder, granule, tablet or capsule, and may additionally include a dispersant or stabilizer.

In addition, the present invention is a mixed extract; cabbage extract; honey; ginger juice; And Stevia; provides a food composition for the prevention, treatment or improvement of gastric diseases caused by Helicobacter pylori containing the mixture as an active ingredient.

The food composition according to the present invention may be formulated in the same manner as the pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, alcoholic beverages, sweets, diet bars, dairy products, meat, chocolate, pizza, ramen, other noodles, gums, ice cream, vitamin complexes, health supplements etc.

The food composition of the present invention is a mixed extract as an active ingredient; cabbage extract; honey; ginger juice; and stevia; as well as ingredients commonly added during food production, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents. Examples of the above-mentioned carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents [taumatine, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is prepared as a drink and beverages, the mixed extract of the present invention; pear juice; and citric acid, fructose liquid, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts in addition to honey.

The present invention is the mixed extract; cabbage extract; honey; ginger juice; And Stevia; provides a health functional food comprising a food composition for the prevention, treatment or improvement of gastric diseases caused by Helicobacter pylori containing as an active ingredient. Health functional food is a mixed extract; cabbage extract; honey; ginger juice; and stevia; is added to food materials such as beverages, teas, spices, gum, and confectionery, or is a food manufactured by encapsulation, powdering, suspension, etc. Unlike drugs, it has the advantage that there are no side effects that may occur when taking the drug for a long time because it uses food as a raw material. The health functional food of the present invention obtained in this way is very useful because it can be ingested on a daily basis. Mixed extracts in such health functional foods; cabbage extract; honey; ginger juice; and stevia; can not be defined uniformly because it varies depending on the type of health functional food, but it can be added in a range that does not impair the original taste of the food, and is usually 0.01 to 50% by weight relative to the target food, preferably is in the range of 0.1 to 20% by weight. In addition, in the case of a health functional food in the form of pills, granules, tablets or capsules, it is usually added in an amount of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a pill, tablet, capsule or beverage.

In addition, the present invention is a mixed extract for the production of a medicament or food for the prevention, treatment or improvement of gastric diseases caused by Helicobacter pylori; cabbage extract; honey; ginger juice; and stevia. mixed extracts as described above; cabbage extract; honey; ginger juice; and stevia may be used for gastric diseases caused by Helicobacter pylori.

In addition, the present invention provides an effective amount of a mixed extract for a mammal; cabbage extract; honey; ginger juice; And it provides a method for improving, preventing or treating gastric diseases caused by Helicobacter pylori, comprising administering a mixture of stevia.

As used herein, the term "mammal" refers to a mammal that is the subject of treatment, observation or experimentation, preferably a human.

As used herein, the term "effective amount" refers to the amount of an active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human as conceived by a researcher, veterinarian, physician or other clinician, which an amount that induces alleviation of the symptoms of a disease or disorder. It is apparent to those skilled in the art that the effective amount and frequency of administration for the active ingredient of the present invention will vary depending on the desired effect. Therefore, the optimal dosage to be administered can be easily determined by those skilled in the art, and the type of disease, the severity of the disease, the content of active ingredients and other components contained in the composition, the type of formulation, and the age, weight, and general health of the patient Condition, sex and diet, administration time, administration route and secretion rate of the composition, treatment period, can be adjusted according to various factors including drugs used concurrently. In the prevention, treatment or improvement method of the present invention, for adults, a mixed extract; cabbage extract; honey; ginger juice; And when the mixture consisting of stevia is administered once to several times a day, it is preferably administered at a dose of 0.001 g/kg to 10 g/kg.

Mixed extract in the treatment method of the present invention; cabbage extract; honey; ginger juice; And a composition comprising a mixture consisting of stevia as an active ingredient can be administered in a conventional manner via oral, rectal, intravenous, intraarterial, intraperitoneal, intramuscular, intrasternal, transdermal, topical, intraocular or intradermal routes. have.

Hereinafter, preferred examples are presented to help the understanding of the present invention, but the following examples are merely illustrative of the present invention, and it will be apparent to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention, It goes without saying that such variations and modifications fall within the scope of the appended claims.

Example 1.

production of thistle

1 part by weight of a flower of thistle, 18.6 parts by weight of a mixture of stem and leaf (stem:leaf = 1:2 weight ratio), and 45 parts by weight of water were mixed and extracted at 95° C. for 24 hours to obtain an extract A, followed by 0.4 parts by weight of thistle root. Extract B was obtained by extracting parts by weight and 45 parts by weight of water at 95° C. for 24 hours, and then lyophilized to prepare powder A and powder B, and then mixed to prepare a mixed extract powder of thistle.

Preparation of Cabbage Extract

After mixing 10 parts by weight of washed cabbage and 40 parts by weight of water, the cabbage extract was prepared by extracting in a bath at 95° C. for 8 hours.

Preparation of mixtures

After mixing the prepared milk thistle and dandelion in a weight ratio of 1:0.42, the mixture and water were mixed in a weight ratio of 1:9 and extracted at 90° C. for 48 hours to prepare a mixed extract. Add 11.2 parts by weight of the cabbage extract and 0.75 parts by weight of ginger juice to 100 parts by weight of the mixed extract and mix at room temperature for 10 minutes, then add 2.5 parts by weight of honey and 0.25 parts by weight of stevia to mix for 10 minutes at room temperature and 0.25 weight of agar By adding parts and mixing for another 5 minutes, a mixture consisting of a mixed extract, cabbage extract, honey, ginger juice and stevia was obtained.

Comparative Example 1. Using only the mixed extract

It was carried out in the same manner as in Example 1, except that cabbage extract, honey, ginger juice and stevia were not used, and only a mixed extract consisting of thistle and dandelion was used.

Comparative Example 2. Cabbage extract omitted

It was carried out in the same manner as in Example 1, but a mixture was obtained without using a cabbage extract.

Comparative Example 3. Ginger juice omitted

A mixture was obtained in the same manner as in Example 1, except that ginger juice was not used.

Comparative Example 3. Honey omitted

A mixture was obtained in the same manner as in Example 1, except that honey was not used.

Comparative Example 4. Omitting thistle

It was carried out in the same manner as in Example 1, except that the mixed extract was obtained without using milk thistle when preparing the mixed extract, and then the cabbage extract, honey, ginger juice and stevia were mixed to obtain a mixture.

<Test Example>

Test Example 1. Helicobacter pylori inhibitory effect

Antibacterial activity against H. pylori (KCTC5335, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea) was measured by the paper disk method (agar diffusion method). The sample was aseptically absorbed by 50 μL of the prepared test solution on a 10 mm paper disk (ADVANTEC, Japan) and dried. H. pylori cultured on an agar medium is suspended in sterile water to 1X10 ⁸ CFU (colony forming unit) or more to form a strain solution. Spread 100 μL of each strain solution on an agar medium without antibiotics added, and test solution Absorb the dried paper disk and attach it to the agar medium on which H. pylori has been smeared. After culturing for 72 hours in a microaerobic state in a CO2 incubator, the size (size, mm) of the clear zone formed around the paper disk as H. pylori grew was measured.

division Growth inhibition ring size (mm) 25 μg 50 μg 100 μg Control (distilled water) 0 0 0 Example 1 28 30 or more 30 or more Comparative Example 1 11 12 14 Comparative Example 2 20 22 22 Comparative Example 3 19 19 20 Comparative Example 4 23 25 26 Comparative Example 5 15 16 18

As shown in Table 1 above, it was confirmed that the mixture prepared according to Example 1 of the present invention further inhibited the growth of Helicobacter pylori (KCTC5335) compared to the mixtures of the control and Comparative Examples 1 to 5.

Hereinafter, a formulation example of a composition containing the powder of the present invention will be described, but the present invention is not intended to limit the present invention, but merely to describe it in detail.

Formulation Example 1. Preparation of powder

500 mg of the mixture powder obtained in Example 1

Lactose 100 mg

talc 10 mg

The above ingredients are mixed and filled in an airtight bag to prepare a powder.

Formulation Example 2. Preparation of tablets

300 mg of the mixture powder obtained in Example 1

100 mg cornstarch

Lactose 100 mg

2 mg magnesium stearate

After mixing the above ingredients, tablets are prepared by tableting according to a conventional manufacturing method of tablets.

Formulation Example 3. Preparation of capsules

200 mg of the mixture powder obtained in Example 1

3 mg of crystalline cellulose

Lactose 14.8 mg

0.2 mg magnesium stearate

According to a conventional capsule preparation method, the above ingredients are mixed and filled in a gelatin capsule to prepare a capsule.

Formulation Example 4. Preparation of injection

600 mg of the mixture powder obtained in Example 1

mannitol 180 mg

2974 mg of sterile distilled water for injection

Na ₂ HPO _4, 12H ₂ O 26 mg

According to a conventional method for preparing injections, the content of the above ingredients per 1 ampoule is prepared.

Formulation Example 5. Preparation of liquid formulation

4 g of the mixture powder obtained in Example 1

10 g isomerized sugar

5 g of mannitol

Purified water appropriate amount

According to a conventional liquid preparation method, each component is added to purified water to dissolve, an appropriate amount of lemon flavor is added, the above components are mixed, purified water is added, the whole is adjusted to 100 g by adding purified water, and then filled in a brown bottle and sterilized to prepare a liquid.

Formulation Example 6. Preparation of granules

1,000 mg of the mixture powder obtained in Example 1

appropriate amount of vitamin mixture

70 μg vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

0.5 mg of vitamin B6

0.2 μg of vitamin B12

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

50 μg folic acid

Calcium pantothenate 0.5 mg

Appropriate amount of inorganic mixture

ferrous sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg calcium carbonate

Magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for granules in a preferred embodiment, but the mixing ratio may be arbitrarily modified. It can be prepared and used in the preparation of a health functional food composition according to a conventional method.

Formulation Example 7. Preparation of functional beverage

1,000 mg of the mixture powder obtained in Example 1

1,000 mg citric acid

100 g of oligosaccharides

2 g of plum concentrate

1 g of taurine

Add purified water to total 900 mL

After mixing the above ingredients according to a conventional health drink manufacturing method, after stirring and heating at 85 ° C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized, and then refrigerated. It is used to prepare the functional beverage composition of the present invention.

Although the composition ratio is prepared by mixing ingredients suitable for relatively favorite beverages in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and national preferences such as demand class, demand country, and use purpose.

Claims (7)

i) Thistle extract powder and dandelion are mixed in a weight ratio of 1:0.2-1 and extracted at 80 to 100° C. to obtain a mixed extract ii) 1 to 30 parts by weight of cabbage extract and ginger juice based on 100 parts by weight of the mixed extract 0.1 to 5 parts by weight is added and mixed, and iii) 0.5 to 10 parts by weight of honey and 0.1 to 5 parts by weight of stevia are added to the mixed mixture of the cabbage extract and ginger juice, and the mixture obtained by mixing is contained as an active ingredient,
The thistle extract powder is an extract obtained by extracting a mixture of flowers, stems and leaves of thistle, and extracts containing a root extract of thistle, wherein the mixture of flowers, stems and leaves and roots are 1:14-25:0.1-0.9. mixed by weight,
A food composition for the prevention or improvement of gastric diseases caused by Helicobacter pylori, characterized in that the powder obtained by drying and pulverizing the residue remaining after extracting the root at 80 to 100°C in hot water is added to the milk thistle extract powder. delete delete According to claim 1, wherein the gastric disease caused by Helicobacter pylori is one or more gastric diseases selected from the group consisting of acute gastritis, chronic gastritis, gastric ulcer, duodenal ulcer, gastric cancer and gastric lymphoma caused by Helicobacter pylori. Food composition for the prevention or improvement of gastric diseases caused by Helicobacter pylori, characterized in that. delete (A) obtaining a mixed extract by mixing and extracting milk thistle extract powder and dandelion in a weight ratio of 1: 0.2-1;
(B) adding and mixing cabbage extract and ginger juice to the mixed extract; and
(C) adding honey and stevia to the mixture in which the cabbage extract and ginger juice are mixed;
1 to 30 parts by weight of cabbage extract, 0.5 to 10 parts by weight of honey, 0.1 to 5 parts by weight of ginger juice, and 0.1 to 5 parts by weight of stevia with respect to 100 parts by weight of the mixed extract,
The thistle extract powder is an extract obtained by extracting a mixture of flowers, stems and leaves of thistle, and extracts containing a root extract of thistle, wherein the mixture of flowers, stems and leaves and roots are 1:14-25:0.1-0.9. mixed by weight,
Preparation of a food composition for the prevention or improvement of gastric diseases caused by Helicobacter pylori, characterized in that the powder obtained by drying and pulverizing the residue remaining after extracting the root at 80 to 100°C in hot water is added to the milk thistle extract powder Way. i) Thistle extract powder and dandelion are mixed in a weight ratio of 1:0.2-1 and extracted at 80 to 100° C. to obtain a mixed extract ii) 1 to 30 parts by weight of cabbage extract and ginger juice based on 100 parts by weight of the mixed extract 0.1 to 5 parts by weight is added and mixed, and iii) 0.5 to 10 parts by weight of honey and 0.1 to 5 parts by weight of stevia are added to the mixed mixture of the cabbage extract and ginger juice, and the mixture obtained by mixing is contained as an active ingredient,
The thistle extract powder is an extract obtained by extracting a mixture of flowers, stems and leaves of thistle, and extracts containing a root extract of thistle, wherein the mixture of flowers, stems and leaves and roots are 1:14-25:0.1-0.9. mixed by weight,
A pharmaceutical composition for the prevention or treatment of gastric diseases caused by Helicobacter pylori, characterized in that the powder obtained by drying and pulverizing the residue remaining after extracting the root at 80 to 100°C in hot water is added to the milk thistle extract powder.