KR102421688B1 - Antibacterial functional cosmetic composition for anti-oxidation, anti-inflammatory and anti-aging having gardenia floride flower extract, pelargonium graveolens extract and salvia officinalis extract - Google Patents

Abstract

The present invention relates to an antibacterial functional cosmetic composition comprising gardenia flower extract, pelargonium graveolens extract, salvia officinalis extract, 3-0-ethyl ascorbic acid, and panthenol as active ingredients, and a method for preparing the same . The cosmetic composition provided in the present invention contains natural ingredients as a main ingredient, so it minimizes skin irritation, effectively inhibits the proliferation of bacteria, exhibits excellent antibacterial activity, and has excellent anti-inflammatory and antioxidant effects, including overall skin improvement effects. It is an antibacterial functional cosmetic composition.

Description

Antibacterial functional cosmetic composition for antioxidant, anti-inflammatory, and anti-aging comprising gardenia flower extract, pelargonium gravelens extract, and Salvia officinalis extract as active ingredients ANTI-AGING HAVING GARDENIA FLORIDE FLOWER EXTRACT, PELARGONIUM GRAVEOLENS EXTRACT AND SALVIA OFFICINALIS EXTRACT}

The present invention relates to an antibacterial functional cosmetic composition comprising a gardenia flower extract, a Pelargonium graveolens extract, and a Salvia officinalis extract as active ingredients. It relates to this improved cosmetic composition and a manufacturing method thereof.

In order to promote personal safety and hygiene from disease, it is particularly important to disinfect and remove viruses from the hands. In the past, hand sanitizer was an essential element for people who are interested in hygiene or health in addition to people in some professions, but recently, due to Corona 19, the importance of hand disinfection has become essential knowledge beyond common sense to the general public. By keeping your hands clean, you can prevent COVID-19 as well as most infectious diseases.

As a result of the World Health Organization (WHO) survey, it is reported that 40% of bacteria are sterilized when washed with water alone, 20% when washed with general soap, and 99.99% when washed with alcohol-based hand sanitizer. However, even with solid soap, according to WHO's hand hygiene guidelines, about 90% of bacteria can be removed by hand washing for 15 seconds and 99% by hand washing for 30 seconds. It should be noted that by breeding there is a risk of re-infection during handwashing or infection during rinsing with contaminated water. In addition, soap has the inconvenience of having to be washed with water after use, and a bigger disadvantage is that a skin moisturizer is used, but due to the action of water washing and surfactant, the skin moisturizer, which should remain on the skin, does not function properly. There is a disadvantage of the prior art being washed out. In particular, it was difficult to clean bacteria or skin contamination in the absence of water.

Therefore, disinfectants containing alcohol have been developed that can exert sterilization power even in the absence of water, and the form is classified into a gel type and a liquid type. Looking at the patent technologies related to these alcohol-based disinfectants, 60-68 vol% of ethanol and 2-5 vol% of isopropanol, gel "High bactericidal composition" containing 0.1-0.3% by weight of carbomer as a topical agent, and 30 to 80% by weight of ethanol according to Korean Patent Registration No. 10-0425943 (published on April 3, 2004); 0.01 to 5% by weight of isopropyl alcohol; 0.01 to 1% by weight of benzalkonium chloride; 0.5 to 6% by weight of at least one skin moisturizer selected from the group consisting of propylene glycol, aloe vera, glycerin, sorbitol, isopropyl myristate and silicone derivatives; and "Lotion-type skin protection disinfectant composition", characterized in that the remainder contains water. However, most of these alcohol-based disinfectants usually contain 60 to 85% alcohol, and up to 95% alcohol. Since the ingredients are volatilized, there is a problem that the skin is still dry, pulling, and crumbly while the moisture and nutrients of the human body and hands to which the composition is applied are volatilized together.

In addition, the skin of the human body, which has become sensitive due to the influence of environmental pollution, is sometimes stimulated by alcohol components, so consumers with sensitive skin prefer non-alcoholic products that do not contain alcohol components.

As the public's interest in personal hygiene such as hand disinfection increases to prevent such infectious diseases and infections, various antibacterial compositions have been developed and manufactured as personal hygiene products, and other chemical additives are added to enhance moisture retention. . When the antibacterial cosmetic composition made based on alcohol is applied to the skin, it dries again at an early time, so the durability of moisturizing is not excellent, and skin irritation may occur due to the use of chemical additives.

Accordingly, the present inventors have led to the present invention by developing a technology including natural ingredients as active ingredients to minimize skin irritation while improving antibacterial functionality, and furthermore, to include excellent anti-inflammatory and antioxidant functions.

Korean Patent Publication No. 10-2124592

The present invention relates to an antibacterial functional cosmetic composition comprising a gardenia flower extract, a pelargonium graveolens extract, and an extract of Salvia officinalis as active ingredients.

It is an object of the present invention to provide an antibacterial functional cosmetic composition comprising a mixed extract of a gardenia flower extract, a pelargonium graveolens extract, and a Salvia officinalis extract, and a method for preparing the same.

However, the technical task to be achieved by the present invention is not limited to the tasks mentioned above, and other tasks not mentioned will be clearly understood by those of ordinary skill in the art from the following description.

According to an embodiment of the present invention, a mixed extract and purified water of the gardenia flower ( Gardenia Florida Flower ) extract, the Pelargonium Graveolens extract and the Salvia Officinalis (Sage) extract There is provided an antibacterial functional cosmetic composition comprising.

In the present invention, the gardenia flower extract, the Pelargonium graveolens extract and the Salvia officinalis extract may be a cosmetic composition mixed in a weight ratio of 1: 0.5-5: 2-7.

In the present invention, the mixed extract may be a cosmetic composition further comprising any one or more of 3-0-Ethyl Ascorbic acid and Panthenol.

In the present invention, the mixed extract may be a cosmetic composition comprising 0.001 to 35% by weight based on the total weight of the cosmetic composition.

In the present invention, the gardenia flower extract, the Pelargonium graveolens extract, the Salvia officinalis extract, the 3-0-ethyl ascorbic acid and the panthenol are 1: 0.5-5: 2-7: 0.05 ~0.3: It may be a cosmetic composition mixed in a weight ratio of 0.1 to 0.8.

In the present invention, the cosmetic composition may be a cosmetic composition comprising at least one selected from the group consisting of a thickener, preservative, stabilizer, solubilizer, surfactant, carrier, and incense burner.

In the present invention, the cosmetic composition may be a cosmetic composition having anti-inflammatory, antioxidant, and anti-aging functions.

According to another embodiment of the present invention, gardenia flower ( Gardenia Florida Flower ), Pelargonium Graveolens ( Pelargonium Graveolens ) and Salvia Officinalis ( Salvia Officinalis ( Sage )) Ultrasonic extraction of raw materials to obtain an extract There is provided a method for producing an antibacterial functional cosmetic composition comprising the step.

In the present invention, the gardenia flower ( Gardenia Florida Flower ) extract, the Pelargonium Graveolens ( Pelargonium Graveolens ) extract and the Salvia Officinalis (Sage) extract 3-0-ethyl ascorbic acid (3-0-Ethyl Ascorbic acid) and panthenol (Panthenol) may be a method for producing an antibacterial functional cosmetic composition comprising the step of mixing any one or more.

The antibacterial functional cosmetic composition provided by the present invention has excellent antibacterial, anti-inflammatory, and anti-aging effects as well as natural ingredients such as gardenia flower extract, pelargonium graveolens extract, and Salvia officinalis extract. To provide an antibacterial functional cosmetic composition with excellent antioxidant and anti-inflammatory activity while minimizing skin irritation by using it as an active ingredient.

Hereinafter, examples will be given to describe in more detail. However, the following examples are illustrative, and the scope of the present invention is not limited thereto.

According to an embodiment of the present invention, a mixed extract and purified water of the gardenia flower ( Gardenia Florida Flower ) extract, the Pelargonium Graveolens extract and the Salvia Officinalis (Sage) extract It relates to an antibacterial functional cosmetic composition comprising.

In the present invention, the mixed extract can be applied to extracts of Gardenia flower, Pelargonium Graveolens and Salvia officinalis after drying and crushing the powder.

The gardenia flower ( Gardenia Florida Flower ) is a plant of the genus Gardenia, and the stem, root, leaf, flower, petal, seed (seed), pulp, fruit peel or fruit of the gardenia flower may be crushed, chopped, or dried, and these It may be used as or mixed, and there is no particular limitation as long as it is commonly used as a gardenia flower containing triterpenoid components as a kind of plant. There are about 250 species of gardenia in the world, and it is distributed in tropical and subtropical regions, and in the case of 5 species in China and one mutant species, all can be used medicinally, and the flowers contain triterpenoid components.

The Pelargonium Graveolens ( Pelargonium Graveolens ) is an aromatic plant of the genus Pelargonium having essential oils on leaves and stems, and the stems, roots, leaves, flowers, petals, seeds (seeds), and flesh of Pelargonium Graveolens. , may be crushed, chopped, or dried fruit peel or fruit, and these may be used alone or mixed, and there is no particular limitation as long as the type of plant is commonly referred to as Pelargonium graveolens. The Pelargonium graveolens is distributed in large areas such as South Africa, Europe, and Asia, and is widely cultivated for herbs in Korea, and is mostly used as a fragrance for perfumes, cosmetics, soaps, and the like, and is also used as a pharmaceutical.

The Salvia Officinalis (Sage) is a plant of the genus Baeaceae, stems, roots, leaves, flowers, petals, seeds (seeds), flesh, fruit skins or fruits of Salvia officinalis. It may be crushed, minced, or dried, and these may be used alone, or may be used in combination, and there is no particular limitation as long as it is commonly used as Salvia officinalis as a kind of plant. The Salvia officinalis is also called medicinal sage, and for medicinal purposes, boiled leaves are known to be used for sore throat and gastroenteritis.

The mixed extract may further include a crude extract, a fraction, or a combination thereof of the gardenia flower extract, pelargonium graveolens extract, salvia officinalis extract.

The crude extract is obtained by contacting the gardenia flower extract, the Pelargonium graveolens extract, and the Salvia officinalis extract with an extraction solvent, in which various substances are mixed, and the fraction is specific for the crude extract. The material containing the component is separated, and the separation may be separation, chromatography, or filtration using an organic solvent, and the chromatography is, for example, silica gel column chromatography, Sephadex LH-20 chromatography, ion exchange. chromatography, affinity chromatography, size exclusion chromatography, HPLC, or a combination thereof.

As used herein, the term "extract" refers to an extract obtained by extraction treatment, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a prepared or purified product of the extract, or a mixture thereof, such as the extract itself and the extract Includes extracts of all formulations that can be formed using

In the present invention, the term "antibacterial function" refers to blocking and inhibiting the growth of microorganisms such as bacteria, bacteria, and fungi, and includes the effect of preventing the growth of bacteria, bacteria, and fungi caused by the external environment, or preventing virus infection. The composition according to the present application may be used for various purposes requiring inhibition of bacteria in addition to the above-described antibacterial function.

The mixed extract comprising the gardenia flower extract of the present invention, the Pelargonium graveolens extract and the Salvia officinalis extract as an active ingredient is 0.001 to 40, 0.001 to 39, 0.001 based on the total weight of the cosmetic composition. to 38, 0.001 to 37, or 0.001 to 36% by weight, and the mixed extract is included in an appropriate amount in the composition to achieve excellent antibacterial activity and stable formulation. It may be included in preferably 0.001 to 35% by weight, but , but is not limited thereto. In the present invention, when the content of the mixed extract is less than 0.001% by weight, the inhibitory effect on bacterial growth is insignificant, and when it exceeds 35% by weight, the desired formulation is difficult and the feeling of use is reduced.

The gardenia flower extract of the present invention, the Pelargonium graveolens extract and the Salvia officinalis extract may be mixed in a weight ratio of 1: 0.5-5: 2-7, preferably 1: 1- 5: May be mixed in a weight ratio of 3 to 7, more preferably 1: 3: 3, 1: 4: 4, 1: 1: may be a cosmetic composition mixed in a weight ratio of 1: 7, but is not limited thereto .

The cosmetic composition is better than the gardenia flower extract, the Pelargonium graveolens extract, and the Salvia officinalis extract by mixing and including the extracts in a certain ratio within the range, rather than including only one of the extracts. It may have anti-proliferative and anti-inflammatory and antioxidant effects.

The mixed extract of the present invention is a cosmetic composition further comprising any one or more of 3-0-ethyl ascorbic acid (3-0-Ethyl Ascorbic acid) and panthenol in terms of exhibiting effective anti-inflammatory and antioxidant effects can be

The 3-0-ethyl ascorbic acid (3-0-Ethyl Ascorbic acid) is a water-soluble vitamin. It is present in citrus fruits, tomatoes, strawberries and many other fruits and vegetables, and can also be obtained synthetically, and its deficiency causes scurvy.

The panthenol (Panthenol) is a water-soluble vitamin that dissolves well in water, and when absorbed into the body as one of the essential nutrients, it is changed into a component called pantothenic acid through a chemical action. Therefore, it can be widely used as a stable ingredient.

The mixed extract comprising the gardenia flower extract of the present invention, the Pelargonium graveolens extract, the Salvia officinalis extract, the 3-0-ethyl ascorbic acid and the panthenol as active ingredients is a cosmetic composition total Based on the weight, it may contain 0.001 to 40, 0.001 to 39, 0.001 to 38, 0.001 to 37, or 0.001 to 36% by weight, and the mixed extract is included in an appropriate amount in the composition to effectively inhibit the proliferation of skin flora. In terms of achieving antimicrobial activity and formulation of a stable viscosity, the composition may be preferably included in an amount of 0.001 to 35% by weight, but is not limited thereto. In the present invention, when the content of the mixed extract is less than 0.001% by weight, the inhibitory effect on bacterial growth is insignificant, and when it exceeds 35% by weight, the viscosity when applying a cream or fixed formulation due to the acidic component 3-O-ethyl ascorbic acid The desired formulation is difficult and the feeling of use is deteriorated. Panthenol may be mixed in a weight ratio of 1: 0.5-5: 2-7: 0.05-0.3: 0.1-0.8, preferably in a weight ratio of 1: 1-5: 3-7: 0.1-0.3: 0.3-0.7 may be mixed, more preferably 1: 3: 3: 0.1: 0.5, 1: 3: 3: 0.2: 0.5, 1: 4: 4: 0.1: 0.5, 1: 4: 4: 0.2: 0.5, 1 It may be a cosmetic composition that can be mixed in a weight ratio of: 1: 7: 0.1: 0.5, 1: 1: 7: 0.2: 0.5, but is not limited thereto.

The gardenia extract may be 3.89 to 11.59, 4 to 11.5, 4.5 to 11.4, 5 to 11.3, 6 to 11.2, or 7 to 11.2% by weight based on the total weight of the cosmetic composition, in terms of maintaining excellent anti-inflammatory and antioxidant effects Depending on the embodiment, it may be preferably 8.3 to 11.11 wt%.

The Pelargonium graveolens extract may be 3.89 to 11.59, 4 to 11.5, 4.5 to 11.4, 5 to 11.3, 6 to 11.2, or 7 to 11.2 wt% based on the total weight of the cosmetic composition, and maintain excellent anti-inflammatory and antioxidant effects According to an embodiment, in terms of that, it may be preferably 8.3 to 11.11 wt%.

The Salvia officinalis extract may be 3.89 to 11.59, 4 to 11.5, 4.5 to 11.4, 5 to 11.3, 6 to 11.2 or 7 to 11.2% by weight relative to the total weight of the cosmetic composition, and maintain excellent anti-inflammatory and antioxidant effects According to an embodiment, in terms of that, it may be preferably 8.3 to 11.11 wt%.

The 3-0-ethyl ascorbic acid is 0.12 to 13.46, 0.2 to 12, 0.3 to 11, 0.4 to 10, 0.5 to 9, 0.6 to 8, 0.7 to 7, 0.8 to 6, 0.7 to 5, relative to the total weight of the cosmetic composition , may be 0.8 to 4, 0.9 to 3, 1.0 to 2% by weight, and preferably 1.11 to 1.89% by weight according to an embodiment in terms of maintaining excellent anti-inflammatory and antioxidant effects.

The panthenol may be 0.23 to 15.55, 1 to 15, 2 to 14, 3 to 13, 4 to 10.5, 5 to 9% by weight, based on the total weight of the cosmetic composition, and in terms of maintaining excellent anti-inflammatory and antioxidant effects, one embodiment Preferably, it may be 5.55 to 8.54 wt% depending on the

The cosmetic composition contains the extracts than when including only one of the gardenia flower extract, the pelargonium gravelens extract, the salvia officinalis extract, the 3-0-ethyl ascorbic acid and the panthenol. By mixing in a certain ratio within the above range, it can have excellent antibacterial activity and anti-inflammatory and antioxidant effects.

The present inventors use the gardenia flower extract as described above, the Pelargonium graveolens extract, the Salvia officinalis extract, the 3-0-ethyl ascorbic acid and the panthenol as active ingredients in a specific compounding ratio or weight ratio. In the case of a cosmetic composition comprising, it was confirmed that it can be used as an antibacterial cosmetic composition by effectively inhibiting the proliferation of skin flora and exhibiting excellent antibacterial activity. In addition, it was specifically confirmed that the composition exhibited excellent anti-inflammatory and anti-aging activities, and thus showed a skin improvement effect.

As used herein, the term “cosmetic composition” refers to an article used in the human body to clean and beautify the human body to add attractiveness, to brighten the appearance, or to maintain or promote health of skin and hair. The cosmetic composition may be for antibacterial.

Here, the skin flora is, for example, Escherichia coli ( E. coli ), Candida albicans ( Candida albicans ), Bacillus subtilis ( Bacillus subtilis ), Staphylococcus aureus ( Staphylococcus aureus, S. aureus ), Aspergillus niger , Pseudomonas aeruginosa , Escherichia coli , Micrococcus lutues ), Klebsiella pneumoniae pnemonia ( Kleb siella pnemonia ) , And Stephylococcus epidermidis ( Staphylococcus epidermidis ) It may be any one or more selected from the group consisting of.

The cosmetic composition of the present invention may be a cosmetic composition comprising any one or more selected from the group consisting of thickeners, preservatives, stabilizers, solubilizers, surfactants, carriers, and incense burners. In the cosmetic composition, other ingredients and functional ingredients that are formulated in conventional cosmetics may be additionally blended.

For example, in terms of allowing formulation of a stable viscosity, preferably 1.0 to 2.0% by weight of a preservative, 0.01 to 0.5% by weight of a thickener, 0.03% by weight of a stabilizer, and the remaining amount is purified water based on the total weight of the cosmetic composition. It may be any one or more selected from. Here, the stabilizing agent may be, for example, a chelating agent.

Other ingredients that may be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, disinfectants, antioxidants, plant extracts, pH adjusters, alcohols, colorants, fragrances, A blood circulation promoter, a cooling agent, an anti-inflammatory agent, purified water, etc. are mentioned.

The cosmetic composition of the present invention may be a cosmetic composition having anti-inflammatory, antioxidant, and anti-aging functions.

The cosmetic composition may be for blocking and inhibiting the reproduction and virus of microorganisms such as bacteria, bacteria, mites, and fungi, and the anti-inflammatory, antioxidant, and anti-aging effects can be interpreted as derived from the effects of antibacterial and antiviral. However, the present invention is not limited thereto.

The skin includes all skin parts of the body including the face, hands, arms, legs, feet, chest, belly, back, buttocks, and scalp.

As used herein, the terms "applying", "administering", and "applying" are used interchangeably and result in at least partial localization of a composition according to one embodiment to a desired site, or route of administration. may mean placing the composition according to an embodiment into an individual by

According to another embodiment of the present invention, gardenia flower ( Gardenia Florida Flower ), Pelargonium Graveolens ( Pelargonium Graveolens ) and Salvia Officinalis ( Salvia Officinalis ( Sage )) Ultrasonic extraction of raw materials to obtain an extract It relates to a method for producing an antibacterial functional cosmetic composition comprising the step.

In the extraction of the present invention, the extraction method is not particularly limited, and extraction can be performed according to the methods commonly used in the art such as hot water extraction, ultrasonic extraction, filtration, reflux extraction, etc., but preferably ultrasonic extraction can

The extraction may be repeated one or more times, for example, 1 to 5 times, 2 to 4 times, or 3 times, and each extraction may be performed in the same way or in a different way. Specifically, for example, each gardenia flower extract, pelargonium graveolens extract, and Salvia officinalis extract are extracted by adding an extraction solvent 1 to 15 times by weight compared to each raw material at 15 to 25° C. for 30 minutes to 3 hours. It may be obtained by low-temperature sonication at a frequency of 20 to 40 kHz, and more specifically, the low-temperature sonication may be performed at a temperature of 17 to 22° C. for 30 minutes to 2 hours, and at a frequency of 22 to 38 kHz.

In the present invention, the type of extraction solvent used for extracting Gardenia flower, Pelargonium Graveolens and Salvia officinalis is not particularly limited, and any solvent known in the art may be used. In the present invention, the extract may be obtained by extraction with water as a hydrophilic solvent, C ₁ to C ₁₀ monohydric and dihydric, and trihydric alcohols or a mixed solvent thereof. In addition, non-limiting examples of the extraction solvent include water; C1-C4 lower alcohols, such as methanol, ethanol, propyl alcohol, and butyl alcohol; and polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; Or a mixture thereof may be used. Specifically, methanol, ethanol, n-propanol, isopropanol, n-butanol, sec-butanol, isobutanol, tert-butanol, butylene glycol is used alone or two or more types are mixed can be used by

The extraction may further include a process of separating the plant residue and the extract by a known method such as filtration, and may include removing the solvent from the obtained extract by a known method such as concentration under reduced pressure. It may also include preparing a dry extract by drying the obtained extract, such as nitrogen drying or freeze-drying. The extract may be in the form of a concentrate, or in a form resolvated with an appropriate solvent such as DMSO if necessary after drying, or in a powdered form by freeze-drying.

The extraction may further include performing silica gel column chromatography or Sephadex LH-20 chromatography on the crude extract.

Drying of the extract in the present invention may be carried out by a known method in the range in which useful components from the harvested plant are not destroyed, for example, it may be carried out by a method of natural drying in the shade. In addition, crushing or pulverization may be pulverized by crushing or pulverizing to an extent that useful components of plants can be sufficiently extracted in the subsequent extraction process. The drying, crushing, or pulverizing processes may be performed in reverse order or repeated if necessary.

The present invention is to be provided based on the following experimental results, and the antibacterial functional cosmetic composition of the present invention is a mixed extract of the gardenia flower extract, the Pelargonium graveolens extract and the Salvia officinalis extract as an active ingredient. It is characterized in that it contains, and if it is in a form including the antibacterial functional cosmetic composition, it can be usefully used in the group related to cosmetics, health functional foods, external preparations for skin, quasi-drugs and pharmaceutical compositions.

Also, in the present invention, the term "active ingredient" means a component that can exhibit the desired activity alone or can exhibit activity together with a carrier that is not active by itself.

The Gardenia Florida Flower extract of the present invention, the Pelargonium Graveolens extract and the Salvia Officinalis (Sage) extract to exert effective anti-inflammatory and antioxidant effects It may be a method for producing an antibacterial functional cosmetic composition comprising mixing any one or more of 3-0-ethyl ascorbic acid (3-0-Ethyl Ascorbic acid) and panthenol in one aspect. For example, the gardenia flower extract, the pelargonium graveolens extract, the Salvia officinalis extract, the 3-0-ethyl ascorbic acid and the panthenol are 1: 0.5-5: 2-7: 0.05-0.3: may be mixed in a weight ratio of 0.1-0.8, preferably 1: 1-5: 3-7: 0.1-0.3: may be mixed in a weight ratio of 0.3-0.7.

Hereinafter, the present invention will be described in detail by the following examples. However, the following examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following examples.

Example

Preparation Example 1: Preparation of Gardenia extract

Take 25 g of raw material (UK) for Gardenia Florida Flower , dry it, cut it into 1 to 5 cm pieces, put it in a container, and use 150 mL of 30 wt % Butylene glycol as an extraction solvent at a temperature of 85 ° C. A 5% gardenia extract was prepared by hot water extraction for 2 hours or ultrasonic extraction at a temperature of 20° C. at a frequency of 30 kHz for 1 hour. Thereafter, the final extract was dissolved in water at a concentration of 1% by weight and used in the following experimental examples.

Preparation Example 2: Preparation of Pelargonium Graveolens Extract

Take 25 g of raw material (UK) for Pelargonium Graveolens , dry it, cut it into pieces of 1 to 5 cm, put it in a container, and use 150 mL of 30 wt% Butylene glycol as an extraction solvent at 85 ° C. 5% Pelargonium graveolens extract was prepared by hot water extraction at a temperature of 2 hours or ultrasonic extraction at a frequency of 30 kHz at a temperature of 20 °C for 1 hour. Thereafter, the final extract was dissolved in water at a concentration of 1% by weight and used in the following experimental examples.

Preparation Example 3: Preparation of Salvia officinalis extract

25 g of raw material (Germany) for Salvia Officinalis (Sage) is taken, dried, cut into pieces of 1 to 5 cm, put in a container, and 30 wt% of butylene glycol 150mL as an extraction solvent A 5% Salvia officinalis extract was prepared by hot water extraction at a temperature of 85 ° C. for 2 hours or ultrasonic extraction at a frequency of 30 kHz at a temperature of 20 ° C. for 1 hour. Thereafter, the final extract was dissolved in water at a concentration of 1% by weight and used in the following experimental examples.

Experimental Example 1: Efficacy evaluation according to the manufacturing method

1-1. Analysis of antibacterial activity of hot water extract and ultrasonic extract

Each of the hot water extract and the ultrasonic extract prepared in Preparation Examples 1 to 3 was subjected to a disk diffusion method with antibacterial activity against Escherichia coli, E. coli and Staphylococcus aureus, S.aureus , a skin flora. (Disc diffusion assay, clear zone) was confirmed.

Each mixture prepared by hot water and ultrasonic extraction according to Preparation Examples 1 to 3 was set as a sample, and 0.025% of tetracycline , an antibacterial substance, was set as a positive control. First, two stock strains ( E. coli, S. aureus ) were activated in a liquid medium, and then primary cultured. After spreading the primary culture solution on a solid medium suitable for each strain (using a sterilized cotton swab), and drying it, a sterilized Paper disc 8mm Thin was attached so that the flat side touched the solid medium. Next, the samples of the positive control group and Preparation Examples 1 to 3 were dispensed on each 20ul Paper disc, incubated at 37°C for 24H, and the final clear zone diameter was measured to confirm the antibacterial activity. The results according to the above antibacterial experiment are shown in Table 1 below.

Extraction method strain type positive control gardenia flower Pelargonium Graveolens Salvia Officinalis hot water E. coli 17 X X X S. aureus 17 X X 10 ultrasonic wave E. coli 17 X X X S. aureus 17 9 9 10

As shown in Table 1, in the case of Preparation Examples 1 to 3 prepared by hot water extraction, only the Salvia officinalis extract of Preparation Example 3 showed antibacterial activity in S. aureus , Preparation Examples 1 to prepared by ultrasonic extraction In all 3 cases, it was confirmed that the antibacterial activity appeared only in S. aureus .

1-2. Confirmation of anti-inflammatory and antioxidant effects of hot water extract and ultrasonic extract

In the case of preparing the extracts of Gardenia flower, Pelargonium Graveolens, and Salvia officinalis by the methods of Preparation Examples 1 to 3, it was determined whether the skin improvement effect, specifically, the anti-inflammatory and antioxidant effects could be changed by the extraction method. An experiment was performed to confirm.

Specifically, in order to confirm the anti-inflammatory effect, an experiment was performed to check whether the generation of nitric oxide could be inhibited, and an experiment to confirm the DPPH free radical scavenging activity was performed to confirm the antioxidant activity. The results for the compositions prepared by hot water or ultrasonic extraction of Preparation Examples 1 to 3, respectively, are shown in Table 2 below.

Extraction method Efficacy (%) gardenia flower Pelargonium Graveolens Salvia Officinalis hot water extract Anti-inflammatory (NO) 48.5 55.1 70.6 Antioxidant (DPPH) 45.8 60.6 55.2 Ultrasonic Extract Anti-inflammatory (NO) 55.6 60.1 86.3 Antioxidant (DPPH) 69.5 88.5 79.5

As shown in Table 2 above, in the anti-inflammatory and antioxidant experiments, it was confirmed that the respective compositions extracted through ultrasonic extraction had higher anti-inflammatory and antioxidant effects than the hot water extract, thereby showing excellent skin improvement effects. Therefore, in the following examples, the skin improvement effect was confirmed by using the ultrasonic extract.

Experimental Example 2: Efficacy evaluation according to the mixing ratio

30% by weight of a mixed extract obtained by mixing the composition prepared by the ultrasonic extraction method of Preparation Examples 1 to 3 at the mixing ratio of Examples 1 to 3 and Comparative Examples 1 to 4 shown in Table 3, 1% by weight of preservative, 0.2% by weight of thickener %, 0.03% by weight of the stabilizer, and the balance of purified water were mixed to prepare a cosmetic composition.

Gardenia extract Pelargonium Graveolens Extract Salvia officinalis extract 3-0-Ethyl Ascorbic Acid panthenol Example 1 One 3 3 0.2 0.5 Example 2 One 4 4 0.2 0.5 Example 3 One One 7 0.2 0.5 Comparative Example 1 One One One 0.1 0.5 Comparative Example 2 One One One 0.4 1.0 Comparative Example 3 One 7 One 0.2 0.5 Comparative Example 4 One 4 8 0.2 0.5

2-1. Analysis of antibacterial activity according to ultrasonic extraction

Each of the mixtures according to Table 3 was subjected to an antibacterial activity against Escherichia coli, E. coli and the skin flora, Staphylococcus aureus, S. aureus. Disc diffusion assay, clear zone ) was confirmed through

Each mixture according to Table 3 was set as a sample, 0.025% of tetracycline , an antibacterial substance, as a positive control, and 30% of BG as a negative control. As a control, a commercially available hand sanitizer consisting of ethanol, glycerin, aloe extract, and purified water (ethanol 62%, Korea Life Sciences Co., Ltd.) was used.

First, two stock strains ( E. coli, S. aureus ) were activated in a liquid medium, and then primary cultured. After spreading the primary culture solution on a solid medium suitable for each strain (using a sterilized cotton swab), and drying it, a sterilized Paper disc 8mm Thin was attached so that the flat side touched the solid medium. Next, a positive control, a negative control, a commercially available hand sanitizer and a sample were dispensed on each 20ul Paper disc, and the final clear zone diameter was measured after incubation at 37°C for 24H to confirm the antibacterial activity. The results according to the above antibacterial experiment are shown in Tables 4 and 5 below.

strain type positive control negative control Example 1 Example 2 Example 3 Hand sanitizer E. coli 13 X 8 11 8 X S. aureus 26 X 11 20 10 X

As shown in Table 4, it was confirmed that all of Examples 1 to 3 exhibited antibacterial activity in E. coli and S. aureus , which is a skin flora.

Specifically, it was confirmed that the mixture of Example 2 exhibited antibacterial activity similar to that of the positive control. However, in the case of a commercially available hand sanitizer, it was confirmed that the result did not show any antibacterial activity against the two strains.

strain type Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 E. coli X X X X S. aureus 10 10 9 10

Referring to Tables 4 to 5 above, according to embodiments of the present invention, gardenia flower extract, pelargonium graveolens extract, salvia officinalis extract, 3-0-ethyl ascorbic acid and panthenol were added to 1 : 0.5~5 : 2~7 : 0.05~0.3 : When the mixing ratio of 0.1~0.8 is satisfied, it can be confirmed that the antibacterial activity is excellent, can In addition, Examples 1 to 3 and Comparative Examples 3 and 4 in which the ratios of 3-0-ethyl ascorbic acid and panthenol were set as fixed values and Comparative Example 1 including different ratios of 3-0-ethyl ascorbic acid and panthenol and 2, it can be confirmed that the ratio of gardenia flower extract, pelargonium gravel olens extract and salvia officinalis extract is more important in antibacterial activity, and pelargonium gravel olens extract and gardenia flower extract and In the case of Example 2 having a high ratio of Salvia officinalis extract content, it can be confirmed that antibacterial activity similar to that of the positive control group appears. Specifically, it can be seen that both Comparative Examples 1 and 2 do not exhibit antibacterial activity in E. coli , and the antibacterial activity in S. aureus is also significantly lower than in Example 2.

In the case of Comparative Example 3, it can be seen that the Pelargonium graveolens extract is out of the mixing ratio of the present invention, and the antibacterial activity is low compared to that of Example. Specifically, it can be seen that E. coli does not exhibit antibacterial activity, and the antibacterial activity of S. aureus is also significantly lower than in Example 2. Therefore, it can be seen that, when the Pelargonium graveolens extract is the mixed blending ratio of the present invention, an excellent antibacterial activity is exhibited against all of the skin flora.

In the case of Comparative Example 4, it can be seen that the Salvia officinalis extract is out of the mixing ratio of the present invention, and the antibacterial activity is generally low compared to Examples 1 to 3. Specifically, when Comparative Example 4 was compared with Example 2, where only the compounding ratio of the Salvia officinalis extract was different, E. coli did not exhibit antibacterial activity, and it was confirmed that the antibacterial activity in S. aureus was also significantly lower. have. Therefore, it can be seen that when the Salvia officinalis extract is the mixed blending ratio of the present invention, excellent antibacterial activity appears against all of the skin flora.

As such, when the skin flora is not properly controlled, there is a problem in that itching, dry skin, and even an inflammatory reaction occur.

In particular, in the case of the comparative examples, only the antibacterial activity of a specific bacterium, S. aureus , was shown, and in the case of the Examples, not only E. coli E. coli but also the antibacterial activity against S. aureus were additionally confirmed. It can be seen that the antibacterial effect of the composition according to the embodiments of the present invention is greater, in which various common bacteria exist on the skin and the antibacterial effect against each of the bacteria can act in a complex manner.

2-2. Analysis of anti-inflammatory and antioxidant effects according to ultrasonic extraction

An experiment was performed to confirm the skin improvement effect, specifically, the anti-inflammatory and antioxidant effect of each of the mixtures according to Table 3 above.

In order to confirm the anti-inflammatory effect, an experiment was performed to check whether the generation of nitric oxide can be inhibited, and an experiment to confirm the DPPH free radical scavenging activity was performed to confirm the antioxidant activity, and ethanol as a control , glycerin, aloe extract, and a commercially available hand sanitizer consisting of purified water (ethanol 62%, Korea Life Sciences Co., Ltd.) was used. Results according to the above anti-inflammatory and antioxidant experiments are shown in Tables 6 and 7 below.

Efficacy (%) Example 1 Example 2 Example 3 Hand sanitizer Anti-inflammatory (NO) 65.2 95.6 96.8 X Antioxidant (DPPH) 90.5 92.5 81.6 X

As shown in Table 6, in the case of the hand sanitizer used as a control example, it was confirmed that antioxidant and anti-inflammatory activity did not appear. could be seen to appear.

Efficacy (%) Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Anti-inflammatory (NO) 60.5 68.1 52.5 50.1 Antioxidant (DPPH) 70.7 80.3 95.2 62.1

Referring to Tables 6 to 7, according to the examples of the present invention, gardenia flower extract, pelargonium graveolens extract, salvia officinalis extract, 3-0-ethyl ascorbic acid and panthenol were added to 1 : 0.5~5 : 2~7 : 0.05~0.3 : When the mixing ratio of 0.1~0.8 is satisfied, it has excellent anti-inflammatory and antioxidant activity, so it can be confirmed that there is a skin improvement function, and the desired viscosity It can be confirmed that it is suitable in the preparation of a liquid formulation of

In addition, Examples 1 to 3 and Comparative Examples 3 and 4 in which the ratios of 3-0-ethyl ascorbic acid and panthenol were set as fixed values, and Comparative Example 1 including different ratios of 3-0-ethyl ascorbic acid and panthenol and 2, it can be confirmed that the ratio of gardenia flower extract, Pelargonium graveolens extract and Salvia officinalis extract is important in anti-inflammatory and antioxidant functions, and it can be seen that Pelargonium graveolens extract and Salvia In the case of Example 2, in which the ratio of the officinalis extract content is all high, it can be seen that the anti-inflammatory and antioxidant effects are the best.

In the case of Comparative Example 3, it can be seen that the Pelargonium Graveolens extract is out of the mixing ratio of the present invention, and the anti-inflammatory effect is significantly lower than in Examples 1 to 3. In addition, since the antioxidant efficacy of Comparative Example 3 was rather high, it was found that the higher the content ratio of Pelargonium graveolens, the better the antioxidant effect was, but there is a problem in that both anti-inflammatory and antioxidant activities are not excellent. Therefore, it can be seen that when the Pelargonium graveolens extract is the mixed blending ratio of the present invention, both the anti-inflammatory and antioxidant activities are excellent and the skin improvement effect is remarkable.

In the case of Comparative Example 4, it can be seen that the Salvia officinalis extract is out of the mixing ratio of the present invention, and the anti-inflammatory and antioxidant effects are remarkably low compared to Examples 1 to 3. Specifically, when compared to Example 2, which differs only in the mixing ratio of Comparative Example 4 and Salvia officinalis extract, it can be seen that the higher the content of the Salvia officinalis extract, the better the anti-inflammatory and antioxidant effect, thus When the ratio of the Via officinalis extract is the mixing ratio of the present invention, it can be seen that the skin improvement effect is remarkable.

Therefore, as can be seen in Tables 4 to 7, gardenia flower extract, pelargonium graveolens extract, salvia officinalis extract, 3-0-ethyl ascorbic acid and panthenol were prepared in Example 1 according to the present invention. When included in a compounding ratio of 3 to 3, as compared to Comparative Examples 1 to 4, not only improved antibacterial activity against all of the skin flora, but also excellent anti-inflammatory and antioxidant efficacy, is useful as an antibacterial composition with a remarkable skin improvement effect It has been confirmed that there is a critical significance that can be used effectively.

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (9)

Gardenia Flower ( Gardenia Florida Flower ) extract, Pelargonium Graveolens ( Pelargonium Graveolens ) Extract and Salvia Officinalis (Sage) containing a mixed extract and purified water of the extract, the gardenia flower extract, the An antibacterial functional cosmetic composition for mixing the Pelargonium Graveolens extract and the Salvia officinalis extract in a weight ratio of 1: 0.5-5: 2-7. delete According to claim 1,
The mixed extract is a cosmetic composition further comprising any one or more of 3-0-ethyl ascorbic acid (3-0-Ethyl Ascorbic acid) and panthenol. 4. The method of claim 3,
The mixed extract is a cosmetic composition comprising 0.001 to 35% by weight based on the total weight of the cosmetic composition. 4. The method of claim 3,
The gardenia flower extract, the pelargonium graveolens extract, the Salvia officinalis extract, the 3-0-ethyl ascorbic acid and the panthenol are 1: 0.5-5: 2-7: 0.05-0.3: A cosmetic composition mixed in a weight ratio of 0.1 to 0.8. According to claim 1,
The cosmetic composition is a cosmetic composition comprising at least one selected from the group consisting of thickeners, preservatives, stabilizers, solubilizers, surfactants, carriers, and fragrances. According to claim 1,
The cosmetic composition is a cosmetic composition having anti-inflammatory, antioxidant, and anti-aging functions. Gardenia Florida Flower , Pelargonium Graveolens , and Salvia Officinalis (Sage)) Ultrasonic extraction of raw materials to obtain an extract and the Gardenia flower extract, the Pella Gonium Graveolens extract and the Salvia officinalis extract 1: 0.5-5: Method for producing an antibacterial functional cosmetic composition comprising the step of mixing in a weight ratio of 2-7. 9. The method of claim 8,
3-0-Ethyl Ascorbic Acid (3-0-Ethyl) in Gardenia Florida Flower Extract, Pelargonium Graveolens Extract and Salvia Officinalis (Sage) Extract Ascorbic acid) and panthenol (Panthenol) method for producing an antibacterial functional cosmetic composition comprising the step of mixing any one or more.